DNS LK-400 User manual

November 1, 2016 (First Edition)
Auto Slide Preparation System Extension Module Package
AS-410 series
・ Read the Operation Manual before using LK-400 for correct use.
・ Keep the Operation Manual near the product for quick reference when needed.
・ The LK-400 option is dedicated to the Auto Slide Preparation System AS-410 series.
LK-400
(Option)
Operation Manual
Ver 0.0.5

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package １
Before using this product
Thank you very much for purchasing LIK-400. Please read this manual before using the product for
correct use.
The product is an expansion option for Auto Slide Preparation System AS-410 or AS-410M
(hereinafter referred to as AS-410). Refer to the AS-410 Operation Manual for the usage and
detailed precautions of the System itself.
< About this Operation Manual >
● The information contained in this Operation Manual is subject to change without prior notice due to
improvements in its performance and functions.
● Reprinting or reproduction of this Operation Manual in whole or in part without prior permission is
prohibited.
● In case of loss of the Operation Manual, please contact DNS for another copy.
● We take great care for preparing this Operation Manual. If, however,any questionable points,
inaccuracies or omissions should be found, please inform us.
●Keep LK-400 Operation Manual near the product for quick reference
when needed.
●Read Operation Manual for Auto Slide Preparation System before using
LK-400. The Sample Identification Option is required to use the LK-400
Option.
●In case of using this system connecting with an external PC for a
pathological diagnosis support system and such, a separate software is
required. Please contact us for the detail.
●Back up important data to another media or server periodically in case
that the PC is damaged.
●Failure to use this Software and other connecting equipments and PC
software for these intended instructions in this manual may
compromise a damage. We shall not be held responsible or liable for
such accident and damage.
●Installing other software to the LK-400 PC may cause issues both to the
software and the LK-400 software and other saved data. DNS is not
responsible for direct and indirect damages or disadvantages to the
customer caused by such action.
!
!
Caution

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ２
For the safe and correct use
This symbol requires or instructs the User to take an action.
Specific details are given in writing near the symbol.
!
!
This sign means that failure to comply with the applicable instructions for
correct use may result in personal injury or material damage.
This sign means that failure to comply with the applicable instructions for
correct use may result in death or serious injury.
!
Warning
!
Caution
This sign indicates an operating procedure to which attention should be paid
to ensure efficient use of this System.
!
Note
This symbol informs the User of a prohibited action. Specific
details are given in writing near the symbol.

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ３
Table of Contents
Before using this product.......................................................................................................................１
For the safe and correct use..................................................................................................................２
1. Product overview ...............................................................................................................................５
1-1 Outline .............................................................................................................................................５
1-2 The process of section preparation.................................................................................................５
1-3 LK-400 data link diagram ................................................................................................................６
1-4 Connection of the Auto Slide Preparation System AS-410 and the LK-400...................................７
1 – 5 Instruction on how to use the LK-400 ........................................................................................８
1 – 5 – 1 Transfering data from an external-system PC through LAN ...............................................８
1 – 5 – 2 Manual input operation method corresponding with the sample tray setting number ........９
2. Connection method........................................................................................................................１０
2 – 1 Connection with the Main Unit ...............................................................................................１０
2 – 2 Connection with external system (PC) ..................................................................................１１
3. Creating Data ...............................................................................................................................１２
3 – 1 Dataflow .................................................................................................................................１２
3 – 2 File..........................................................................................................................................１３
3 – 3 Data format ............................................................................................................................１３
4. The start-up and the configuration of LK-400 application......................................................１５
4 – 1 The screen configuration of LK-400 application....................................................................１５
4 – 2 Start-up the Main Unit............................................................................................................１６
4 – 3 Start-up of the LK-400 application .........................................................................................１６
4 – 4 Log in .....................................................................................................................................１６
4 – 5 Setting and confirmation of LK-400 .......................................................................................１７
4 – 5 – 1 Input of the sectioning information (Stain information)....................................................１７
4 – 5 – 2 User Setting ...................................................................................................................２０
4 – 5 – 3 Device Informationetting..................................................................................................２１
4 – 5 – 4 Running History ...............................................................................................................２１
4 – 5 – 5 Error History.....................................................................................................................２２
4 – 5 – 6 Termination of the LK-400................................................................................................２２
5. Auto Sectioning Mode operation ...............................................................................................２３
5 – 1 Descriptions of the screen .....................................................................................................２３
5 – 2 Sample setting .......................................................................................................................２４
5 – 3 Automatic start .......................................................................................................................２６
6. Retry Mode operation..................................................................................................................２８
6 – 1 Description of the screen .......................................................................................................２８
6 – 2 Sample setting .......................................................................................................................２８
6 – 3 Retry Mode operation start ....................................................................................................２９
7 Manual Input Mode ....................................................................................................................３１
7 – 1 Description of the Manual Input Mode .....................................................................................３１
7 – 2 Manual Input Mode operation start........................................................................................３２
7 – 2 – 1 Sectioning information input method (Method by the barcode reader) ...........................３２
7 – 2 – 2 Input method of the sectioning information (Manual Input method)................................３２
7 – 2 – 3 Block setting.....................................................................................................................３３

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ４
7 – 2 – 4 Start sectioning ................................................................................................................３４
8 Troubleshooting.........................................................................................................................３６
9 Specifications.............................................................................................................................４０
9 – 1 Specification table..................................................................................................................４０
9 – 2 Available characters for printing and slide printing range .....................................................４１
9 – 3 Available barcode types for reading ......................................................................................４２
10 Warranty and Exemption.........................................................................................................４３
11 Contact for inquiries ................................................................................................................４３

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ５
1. Product overview
1 – 1 Outline
This Option Package (LK-400) is an extension package which can be connected to the Auto Slide
Preparation System (AS-410) Main Unit and operates sectioning process with a LAN connection
automatically. Users can enter sectioning data or printing information, priviously set with the touch
panel of the AS-410 Main Unit, with the LK-400 system installed in the designated PC, and monitor
automated sectioning process. This extension package enables coordinated automatic section
preparation operation between the external systam and the AS-410.
The LK-400 program has two application methods:
Method 1- The System reads barcodes printed on the sample cassette placed in the AS-410, then
operate the section preparation based on the saved condition data on the PC.
Method 2- The System executes the section preparation operation based on the manual input data
and sample tray numbers.
By connecting with an external system, such as a pathological diagnosis support system sold by
software manufacturers, the LK-400 program enables a coordinated automatic slide glass preparation
with the external system. It requires simply to save the necessary sectioning information onto the
shared data folder and set sample block cassettes with barcodes.
1 – 2 The process of section preparation
①Sample setting
Place the sample cassette with printed barcode on the AS-410 sample tray.
②Barcode reading of the sample
The Sample Identification Function reads the barcode printed on sample casettes. The read data
is transferred to the LK-400 PC and system refers to the registered data.
③Sectioning of the sample
The AS-410 acquires the registered data of each sample from the LK-400 PC; the system sections
each sample automatically with the individually specified conditions, e.g. sectioning thickness and
number of the sections to cut.

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ６
④Printing on slides
The AS-410 acquires the contents of the data registered for each sample in the LK-400 PC, and
automatically prints it on the slide glass (sample number, name, QR code, etc.).
⑤Data matching check
The sample management function of AS-410 checks the QR code printed on the slide glass
against the barcode information of the sample cassette.
⑥Storing the slide in the Slide Collection Box
The specified number of tissue sections cut from a sample block are mounted on slides and
stored in the slide collection box.
1 – 3 LK-400 data link diagram
LK-400
Data matching
Sample identification
information
・Cassette barcode
Sectioning
information
・Sectioning thickness
・Number of sections
・Slide type
Print information
・Specimen number
・Slide number
・Name
・Stain name
・Department
・Tissue name
Sample block information
・Sample set number
・Cassette barcode
Sectioning , Slide printing
Sectioning condition
・Sample set number
・Sectioning number
・Sectioning thickness
・Slide type
Print information
・Specimen number
・Slide number
・Name
・Stain name
・Department
・Institution name
Data input
Sample Information
・Sample number
・Name
・Stain name
・Department
・Tissue name
・
Sectioning thickness
・Number of sections
・Block number
AS-410
External system
(e.g. Pathological
diagnosis support
system)

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ７
1 – 4 Connection of the Auto Slide Preparation System AS-410 and the LK-400
Main Unit
BS-24
24-Sample Feeding
Unit
SS (Option)
400-Slide Feeding
Expansion Unit
BR (Option)
Sample
Identification (Print,
Barcode reading)
FS (Option)
Auto Trimming
(Sample surface
image detection)
96-Sample Feeding Unit
100-Slide Collection Box Dry Chamber (Option)
DU-100
400-Slide Collection Box
Dry Chamber (Option)
DU-400
PC
USB
Touch panel
・LK-400 requires to have the following units and options: Main Unit, Dry Chamber
(DU-100 or DU-400) and Sample Identification (BR) option.
・In case of connecting with an external system, customer needs to coordinate with
the system manufacturer.
・Please contact us for connection, setting and change of the LK-400.
PC
（LK-400 Software）
LAN 1
Handy
barcode
reader
External system
(PC)
LK-400
Option
Expansion
Package
LAN port
LAN port
LAN 2
!
Caution

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ８
AS-connected PC and
Software
Cassette code
Sample information
Sectioning information
LK-400
Slide preparation
system
Printed slide
AS-410
External system server
LAN
Control terminal PC
Barcode reader
Barcode
Numbers,
Name,
…
Cassette printer
(barcode)
Set onto the Main
Unit
External system
LAN (dedicated line)
LAN (dedicated line)
Note: Except the LK-400, an external
system and a cassette printer are
required separately.
LK-400 PC
Auto Sectioning Mode
1 – 5 How to use LK-400
1 – 5 – 1 Transfering data from an external-system PC through LAN
(1) Print the sample identification number (barcode) on the sample embedded cassette from the
external system terminal PC or other device using a cassette printer.
(2) Turn ON the power of LK-400 PC and start up the system.
(3) Create a file of the sample identification number (barcode) read by the terminal PC of external
system of the necessary information for the section preparation, e.g. sectioning condition, slide
type, slide printing, etc. Then save the file information to the shared folder of this LK-400 PC.
(4) Turn ON the power of AS-410 Main Unit and execute the initialization.
(5) Start-up LK-400 Software; select the Auto Sectioning Mode after logging in.
(6) Place the embedded sample cassette discribed in the above step (1) onto the sample tray in the
AS-410 Main Unit.
(7) Execute the section preparation followed by the touch panel menu of AS-410 by online.
(8) After the section preparation is completed, collect the prepared slides stored in the slide collection
box.
(9) Repeat the process from (6) to (8) to perform the section preparation continuously.
Connection configuration example