DNS LK-400 User manual

November 1, 2016 (First Edition)
Auto Slide Preparation System Extension Module Package
AS-410 series
・ Read the Operation Manual before using LK-400 for correct use.
・ Keep the Operation Manual near the product for quick reference when needed.
・ The LK-400 option is dedicated to the Auto Slide Preparation System AS-410 series.
LK-400
(Option)
Operation Manual
Ver 0.0.5

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package １
Before using this product
Thank you very much for purchasing LIK-400. Please read this manual before using the product for
correct use.
The product is an expansion option for Auto Slide Preparation System AS-410 or AS-410M
(hereinafter referred to as AS-410). Refer to the AS-410 Operation Manual for the usage and
detailed precautions of the System itself.
< About this Operation Manual >
● The information contained in this Operation Manual is subject to change without prior notice due to
improvements in its performance and functions.
● Reprinting or reproduction of this Operation Manual in whole or in part without prior permission is
prohibited.
● In case of loss of the Operation Manual, please contact DNS for another copy.
● We take great care for preparing this Operation Manual. If, however,any questionable points,
inaccuracies or omissions should be found, please inform us.
●Keep LK-400 Operation Manual near the product for quick reference
when needed.
●Read Operation Manual for Auto Slide Preparation System before using
LK-400. The Sample Identification Option is required to use the LK-400
Option.
●In case of using this system connecting with an external PC for a
pathological diagnosis support system and such, a separate software is
required. Please contact us for the detail.
●Back up important data to another media or server periodically in case
that the PC is damaged.
●Failure to use this Software and other connecting equipments and PC
software for these intended instructions in this manual may
compromise a damage. We shall not be held responsible or liable for
such accident and damage.
●Installing other software to the LK-400 PC may cause issues both to the
software and the LK-400 software and other saved data. DNS is not
responsible for direct and indirect damages or disadvantages to the
customer caused by such action.
!
!
Caution

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ２
For the safe and correct use
This symbol requires or instructs the User to take an action.
Specific details are given in writing near the symbol.
!
!
This sign means that failure to comply with the applicable instructions for
correct use may result in personal injury or material damage.
This sign means that failure to comply with the applicable instructions for
correct use may result in death or serious injury.
!
Warning
!
Caution
This sign indicates an operating procedure to which attention should be paid
to ensure efficient use of this System.
!
Note
This symbol informs the User of a prohibited action. Specific
details are given in writing near the symbol.

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ３
Table of Contents
Before using this product.......................................................................................................................１
For the safe and correct use..................................................................................................................２
1. Product overview ...............................................................................................................................５
1-1 Outline .............................................................................................................................................５
1-2 The process of section preparation.................................................................................................５
1-3 LK-400 data link diagram ................................................................................................................６
1-4 Connection of the Auto Slide Preparation System AS-410 and the LK-400...................................７
1 – 5 Instruction on how to use the LK-400 ........................................................................................８
1 – 5 – 1 Transfering data from an external-system PC through LAN ...............................................８
1 – 5 – 2 Manual input operation method corresponding with the sample tray setting number ........９
2. Connection method........................................................................................................................１０
2 – 1 Connection with the Main Unit ...............................................................................................１０
2 – 2 Connection with external system (PC) ..................................................................................１１
3. Creating Data ...............................................................................................................................１２
3 – 1 Dataflow .................................................................................................................................１２
3 – 2 File..........................................................................................................................................１３
3 – 3 Data format ............................................................................................................................１３
4. The start-up and the configuration of LK-400 application......................................................１５
4 – 1 The screen configuration of LK-400 application....................................................................１５
4 – 2 Start-up the Main Unit............................................................................................................１６
4 – 3 Start-up of the LK-400 application .........................................................................................１６
4 – 4 Log in .....................................................................................................................................１６
4 – 5 Setting and confirmation of LK-400 .......................................................................................１７
4 – 5 – 1 Input of the sectioning information (Stain information)....................................................１７
4 – 5 – 2 User Setting ...................................................................................................................２０
4 – 5 – 3 Device Informationetting..................................................................................................２１
4 – 5 – 4 Running History ...............................................................................................................２１
4 – 5 – 5 Error History.....................................................................................................................２２
4 – 5 – 6 Termination of the LK-400................................................................................................２２
5. Auto Sectioning Mode operation ...............................................................................................２３
5 – 1 Descriptions of the screen .....................................................................................................２３
5 – 2 Sample setting .......................................................................................................................２４
5 – 3 Automatic start .......................................................................................................................２６
6. Retry Mode operation..................................................................................................................２８
6 – 1 Description of the screen .......................................................................................................２８
6 – 2 Sample setting .......................................................................................................................２８
6 – 3 Retry Mode operation start ....................................................................................................２９
7 Manual Input Mode ....................................................................................................................３１
7 – 1 Description of the Manual Input Mode .....................................................................................３１
7 – 2 Manual Input Mode operation start........................................................................................３２
7 – 2 – 1 Sectioning information input method (Method by the barcode reader) ...........................３２
7 – 2 – 2 Input method of the sectioning information (Manual Input method)................................３２
7 – 2 – 3 Block setting.....................................................................................................................３３

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ４
7 – 2 – 4 Start sectioning ................................................................................................................３４
8 Troubleshooting.........................................................................................................................３６
9 Specifications.............................................................................................................................４０
9 – 1 Specification table..................................................................................................................４０
9 – 2 Available characters for printing and slide printing range .....................................................４１
9 – 3 Available barcode types for reading ......................................................................................４２
10 Warranty and Exemption.........................................................................................................４３
11 Contact for inquiries ................................................................................................................４３

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ５
1. Product overview
1 – 1 Outline
This Option Package (LK-400) is an extension package which can be connected to the Auto Slide
Preparation System (AS-410) Main Unit and operates sectioning process with a LAN connection
automatically. Users can enter sectioning data or printing information, priviously set with the touch
panel of the AS-410 Main Unit, with the LK-400 system installed in the designated PC, and monitor
automated sectioning process. This extension package enables coordinated automatic section
preparation operation between the external systam and the AS-410.
The LK-400 program has two application methods:
Method 1- The System reads barcodes printed on the sample cassette placed in the AS-410, then
operate the section preparation based on the saved condition data on the PC.
Method 2- The System executes the section preparation operation based on the manual input data
and sample tray numbers.
By connecting with an external system, such as a pathological diagnosis support system sold by
software manufacturers, the LK-400 program enables a coordinated automatic slide glass preparation
with the external system. It requires simply to save the necessary sectioning information onto the
shared data folder and set sample block cassettes with barcodes.
1 – 2 The process of section preparation
①Sample setting
Place the sample cassette with printed barcode on the AS-410 sample tray.
②Barcode reading of the sample
The Sample Identification Function reads the barcode printed on sample casettes. The read data
is transferred to the LK-400 PC and system refers to the registered data.
③Sectioning of the sample
The AS-410 acquires the registered data of each sample from the LK-400 PC; the system sections
each sample automatically with the individually specified conditions, e.g. sectioning thickness and
number of the sections to cut.

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ６
④Printing on slides
The AS-410 acquires the contents of the data registered for each sample in the LK-400 PC, and
automatically prints it on the slide glass (sample number, name, QR code, etc.).
⑤Data matching check
The sample management function of AS-410 checks the QR code printed on the slide glass
against the barcode information of the sample cassette.
⑥Storing the slide in the Slide Collection Box
The specified number of tissue sections cut from a sample block are mounted on slides and
stored in the slide collection box.
1 – 3 LK-400 data link diagram
LK-400
Data matching
Sample identification
information
・Cassette barcode
Sectioning
information
・Sectioning thickness
・Number of sections
・Slide type
Print information
・Specimen number
・Slide number
・Name
・Stain name
・Department
・Tissue name
Sample block information
・Sample set number
・Cassette barcode
Sectioning , Slide printing
Sectioning condition
・Sample set number
・Sectioning number
・Sectioning thickness
・Slide type
Print information
・Specimen number
・Slide number
・Name
・Stain name
・Department
・Institution name
Data input
Sample Information
・Sample number
・Name
・Stain name
・Department
・Tissue name
・
Sectioning thickness
・Number of sections
・Block number
AS-410
External system
(e.g. Pathological
diagnosis support
system)

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ７
1 – 4 Connection of the Auto Slide Preparation System AS-410 and the LK-400
Main Unit
BS-24
24-Sample Feeding
Unit
SS (Option)
400-Slide Feeding
Expansion Unit
BR (Option)
Sample
Identification (Print,
Barcode reading)
FS (Option)
Auto Trimming
(Sample surface
image detection)
96-Sample Feeding Unit
100-Slide Collection Box Dry Chamber (Option)
DU-100
400-Slide Collection Box
Dry Chamber (Option)
DU-400
PC
USB
Touch panel
・LK-400 requires to have the following units and options: Main Unit, Dry Chamber
(DU-100 or DU-400) and Sample Identification (BR) option.
・In case of connecting with an external system, customer needs to coordinate with
the system manufacturer.
・Please contact us for connection, setting and change of the LK-400.
PC
（LK-400 Software）
LAN 1
Handy
barcode
reader
External system
(PC)
LK-400
Option
Expansion
Package
LAN port
LAN port
LAN 2
!
Caution

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ８
AS-connected PC and
Software
Cassette code
Sample information
Sectioning information
LK-400
Slide preparation
system
Printed slide
AS-410
External system server
LAN
Control terminal PC
Barcode reader
Barcode
Numbers,
Name,
…
Cassette printer
(barcode)
Set onto the Main
Unit
External system
LAN (dedicated line)
LAN (dedicated line)
Note: Except the LK-400, an external
system and a cassette printer are
required separately.
LK-400 PC
Auto Sectioning Mode
1 – 5 How to use LK-400
1 – 5 – 1 Transfering data from an external-system PC through LAN
(1) Print the sample identification number (barcode) on the sample embedded cassette from the
external system terminal PC or other device using a cassette printer.
(2) Turn ON the power of LK-400 PC and start up the system.
(3) Create a file of the sample identification number (barcode) read by the terminal PC of external
system of the necessary information for the section preparation, e.g. sectioning condition, slide
type, slide printing, etc. Then save the file information to the shared folder of this LK-400 PC.
(4) Turn ON the power of AS-410 Main Unit and execute the initialization.
(5) Start-up LK-400 Software; select the Auto Sectioning Mode after logging in.
(6) Place the embedded sample cassette discribed in the above step (1) onto the sample tray in the
AS-410 Main Unit.
(7) Execute the section preparation followed by the touch panel menu of AS-410 by online.
(8) After the section preparation is completed, collect the prepared slides stored in the slide collection
box.
(9) Repeat the process from (6) to (8) to perform the section preparation continuously.
Connection configuration example

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package ９
AS-connected PC
Setting number
Sectioning information
LK-400
Sampe cassette
Slide print
AS-410
Handy barcode reader
(executable for manual
input)
LAN (dedicated line)
(slide preparation
system)
LK-400 PC
Manual Input Mode
1 – 5 – 2 Manual input operation method corresponding with the sample tray setting number
(1) Turn ON the AS-410 Main Unit and execute initialization.
(2) Turn ON the power of LK-400 PC; start-up the system.
(3) Start up LK-400 Software. Log in, then select the Manual Input Mode.
(4) Place the embedded sample cassette on the sample tray of AS-410.
(5) Enter the necessary information in the LK-400 PC, e.g. sectioning condition, slide type, slide print,
etc.
(6) Execute section preparation from the Main menu of the AS-410 touch panel through online.
(7) After the section preparation is completed, collect the prepared slides stored in the slide collection
box.
(8) Change the sectioning condition, slide type and slide print information if necessary to continue
operation; repeat the process of (5) through (7) to perform the Manual Input Mode of LK-400.
Connection configuration example

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package １０
2. Connection method
2 – 1 Connection with the Main Unit
The following shows the Main Unit (auto-slide preparation system) and the LK-400 PC placement.
Placement of AS-410 and LK-400
Connect the AS-410 and the LK-400 PC with supported LAN cable (crossing cable).
LK-400 PC
AS-410 Main Unit
This example shows the System with
BS-96, DU-400, SS, FS and BR
options.
Expansion LAN port
LAN port
LK-400 PC LAN
Back of the AS-410 Main Unit
・Ensure to use the supported crossing cable for the LAN cable to connect the
AS-410 and the PC. Using other cable may cause the system operation failure.
!
Caution

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package １１
2 – 2 Connection with external system (PC)
In case of connecting the LAN with an external system such as a server of the pathological diagnosis
support system and a terminal PC,connect the expanded LAN port and the LAN hub.
LK-400 PC (LAN connection)
Expanded LAN port
・Some settings of fire walls or autivirus softwares may prevent the correct
operation of the system.
!
Caution

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package １２
Dataflow of the LK-400
3. Creating Data
3 – 1 Dataflow
The necessary data is collected from the database informaion of the ShareData (∖shared folder)
written from an external system. The CSV file saved in the shared folder has respective sample
information such as sectioning condition and print information; AS-410 System operates the data
communication for the described information.
After the section preparation, the CSV file of the completed block with Auto Sectioning Mode
on-line will be saved in the temporary folder ( ￥TmpDtata).
① When the section preparation by AS-410 starts with on-line mode, the system searches the
relevant file from the shared folder which is matching with the sample (cassette) code placed in the
AS-410. The system refers shared folder for the Auto Sectioning Mode; the system refers
temporary folder for the Retry Mode and the Manual Input mode.
② Regarding the files in the shared folder, the system copy the block files to be sectioned in the
automatic sectioning mode to the temp folder and then read the sectioning information.
③ The section preparation information of the sample block placed in the AS-410 will be displayed
on the LK-400.
Sample No. A1
Sample No. B2
Sample No. C3
Writing
ID
Name
…
File
①′ File verification (Manual Input Mode)
Shared folder
（∖ShareData）
Block information
Specified folder
1
①File verification (Auto Sectioning Mode)
②Copy
Temporary folder
(∖TmpDtata)
A１：
ID,Name,…
B２：
ID,Name,…
C３：ID,Name,
…
Necessary information
C3: ID, Name…
Sectioning
info.
C3: 1/3
Error info.
ERR: xxxx...
2012.01.10,C3…
2012.01.10,C3…
2012.01.10,…
2012.01.11,…
2012.02.25…
Specified folder
2
④Sectioning info. saving
⑤Error info. saving
⑥ File deletion (Auto Sectioning Mode)
LK-400
External system
AS-410

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package １３
③ After the slide preparation operation completes, the system creates the stored (collected) slide
information in the Running History. The Running History information can be saved in a specified file
as CSV format.
⑤ The system creates an Error History if system error occurred during the remote connection. Such
Error History can be saved in the specified file as CSV format; however, the PC error and the
caution error from the Main Unit will not be recorded in the Error History. Refer to the 5-1 “Caution
error” or the 5-3 “Error code table” on Chapter 5 of the Operation Manual for more detail.
⑥ When the section preparation completes, the data of the corresponding block will be deleted
from the shared folder.
3 – 2 File
The System verifies the file name and the barcode saved in the shared folder for the automatic file
reading. Create the file with the corresponding name as CSV format.
3 – 3 Data format
Data format: CSV file
File name: Barcode number of the sample block
Data information: The information can be registered up to 400 lines at most for each block (1file),
e.g. stain code, stain name, numbers, etc.
・The hard disk of the PC has the limited capacity. Ensure to backup the file
periodically, about once in half a year. Delete unnecessary file from the shared folder
by using Explorer of the OS, and the like.
Example:
Cassette barcode:
KB-20130101－１
File name:
KB-20130101－１.csv
Barcode
!
Caution

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package １４
【Data descriptions】
Control number
：Serial number when the order is issued.
(Initial value: arbitrary)
* QR code (print)
：QR code number printed on a slide
Sample number
：Sample number
Name 1
：Name
Name 2
：Name
* Sample number
：Sample identification number
Block number
：Block number of the sample
* Stain code
：Identification code of the stain type
Stain name
：Stain name
Number
：Numbers to be sliced
Thickness
：Thickness of the sectioning section（μm）
Sex
：Sex
Date of birth
：Date of birth
Age
：Age
Tissue name
：Name of the tissue (part) of the block
Comment
：Institutional department
Date of issue
：Date of issue of registration order
Note:
1) *mark is required to enter.
2) Except the *mark, a category can be omitted. However, such category needs to be marked
with comma.
3) In case of the thickness omission, the registered thickness of the stain table will bevalid.
Example of the file making:
File name
A160918101.csv
Data contents
1：A160918101, ,XXXX, ,A16-0918,1,0, ,1, , , , , ,GAS,20160924
2：A160918102, ,XXXX, ,A16-0918,1,0, ,1, , , , , ,GAS,20160924
Control
no.
2D code (print)
Name 1
Sample No.
Block No.
Stain code
Number
Commen
t
Date of issue

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package １５
4. The start-up and the configuration of LK-400 application
The following shows the start-up and the configuration of LK-400 personal computer.
4 – 1 The screen configuration of LK-400 application
The following configuration are the screen images of the controlled menu.
Configuration screens of the LK-400 application
Stain
Information
User
Setting
Device
Information
Error
History
Running
History
Auto
Sectionning
Mode
Manual
Input
Mode
Retry
Mode
Exit
Log in
Main menu

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package １６
4 – 2 Starting up the Main Unit
Turn ON the power switches of the AS-410 and the LK-400 PC; boot up the both system.
4 – 3 Starting up LK-400 application
Click the icon of LK-400 on the desktop to start-up the Software.
4 – 4 Log in
The log-in screen appears after the LK-400 application starts. Enter the pre-registered ID and
password to be able to operate each mode. Ensure that the LAN cable of the AS-410 and the LK-400
PC is connected properly.
To change the user’s registration or the stain information registration, enter the administer-autorized
User ID and password, then click Login button.
⚫Change the password from the initial value as needed for the security.
⚫Ensure to keep and save the ID and the Password in a safe place. The system
may not be able to start again without the information.
Initial value
User ID ： admin
Password： pass (half size)
*User ID and password are
case-sensitive.
AS-410
power switch
LK-400 PC power switch
!
Caution
Login Screen
Start-up icon

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package １７
4 – 5 Setting and confirmation of LK-400
(1) Main Menu will be shown on the screen
4 – 5 – 1 Inputting sectioning information (Stain information)
It is necessary to create a stain table beforehand to use the LK-400 properly. The information is
installed with the optimal setting. In case if a user needs to modify the setting, please proceed with
the following steps.
Log in by the authorized administer to change, e.g. addition and deletion of the stain information.
LK-400 distributes the saved file informationuch as the stain code, the sectioning thickness and the
slide type automatically. Input stain name, sectioning thickness and slide type which are
corresponding with the stain code. Originally, the information is installed with the optimall setting.
(1) Adding stain information
2. Stain Information screen wil display; then
click “Add.”
1. Click Stain Information prompt on the LK-400 Main
menu.
Register and confirm the following items.
・Stain Information
・User Setting
・Device Information
・Running History
・Error History
Stain Informationcreen
LK-400 Main menu

LK-400 Operation Manual
Auto Slide Preparation System Extension Module Package １８
⚫Make sure to click the Save
to register. (It will not be registered without
clicking this button.)
⚫
In addition, change and deletion cannot be executed without the authorized
administer logged on.
⚫
A slide glass error occurs if the name of the registered slide type is not
matched with the registered slide name in the Main Unit. Ensure to input the
registered slide name.
!
Caution
3. The Add stain Information screen
appears. Enter the following stain
information.
Stain Code : 0 to 999
Stain Type :The character inputted on the slide.
Thickness :3 to 10
Glass Type ：The slide name registered on the
AS-410.
4. Click OK after inputting the above
information.
Add Stain Informationcreen
Stain Informationcreen