Drawell Water Jacket User manual

Drawell International Technology Limited
Web : www.drawell.com.cn Tel: [phone] Email : [email protected].cn
C O 2C e l l I n c u b a t o r
（Water Jacket）
U s e r M a n u a l
Drawell International Technology Limited
Chongqing Drawell Instrument Co., Ltd.
Shanghai Drawell Scientific Instrument Co.,Ltd.
Chongqing Center : Suite 2705,Building No.12,Shiyou Road No.1, Yuzhong District,
Chongqing, China.
Shanghai Office : Suite 1117,Lane561 XiuChuan Rd.,PuDong New Area,Shanghai,
China
Homepage : www.drawell.com.cn
Tel : [phone]
Email : [email protected]

Drawell International Technology Limited
Web : www.drawell.com.cn Tel: [phone] Email : [email protected].cn
I. Application range
The CO2incubator is used for incubation of biology cells, tissues, bacteria in modern
medicine, pharmacy, biochemist try, agricultural science research and industry.
II. Features
1. The equipment adopts high-quality insulation materials insulation and jacket type, the
tank is equipped with air duct. In the chamber it’s equipped with electric fan for forced
air convection which improves the temperature uniformity and CO2concentration
uniformity.
2. The proportion of CO2 gas and air can arbitrary choice based on demand, using direct
reading type belt needle valve type glass rotameter, Small beautiful and accurate
calibration.
3. In order to avoid contamination effectively, gas into the box body internal filtering and
sterilizing, and the is a germicidal lamps in the chamber at the same time.( Don't open the
germicidal lamp when cultures inside). The air by the electromagnetic pump gas
4. When the door is opened, the fan automatic shut-off, and the heat will stop. Reduce the
amount of pollution caused by the entering air. In order to reduce the over temperature,
shut the door after about 10 min (time can be used according to actual situation set) start
heating.
5. Temperature use by microcomputer system of data analysis and intelligent PID control,
high precision, strong anti-jamming capability. Using three probe to control,
temperature、water and door, which can let the studio’s temperature have high precision
small fluctuation
6. Light touch switch, portable and flexible.
7. Individual door temperature control to reduce the influence of changing ambient
temperature on the chamber thermal system and to avoid the frost of outer glass door.
8. Natural vapor humidifying system to keep ideal humidity.
9. The inner temperature and set parameters, all adopt digital display. Door heating, water
heating, lighting, germicidal lamp, air pump, high water level, water shortage has LED
indicates intuitive, clear
10. Multiple protection function for overheating, gas stopping, etc. to ensure the safety
operation of the equipment
III. Main technical parameters.
Items
DW-WJ-2
DW-WJ-2-160
Volume (L)
80
160
Temperature range(℃)
RT+5～60
Temperature fluctuation
(℃)
≤±0.2
Temperature uniformity
(℃)
≤±0.3
Timing Range
1～9999min or no timing
Flow
range
Air ml/min
160～1600
CO2ml/min
10～100
Power supply
AC220V 50Hz

Drawell International Technology Limited
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Power consumption(W)
600
900
Chamber size （cm3）
41×40.3×51
50.5×45.3×70
Outer size （cm3）
57×65.5×88.5
68.5×69.5×107.5
N.W./G.W. (kg)
60/90
80/115
IV. Installation.
1. The equipment should be placed in a dry, even environment without toxic gas. Direct
sunshine should be avoided. Enough room is spared around the equipment for maintenance.
2. The 99.9% high purity CO2cylinder with pressure reducing valve should be equipped for
the working of this incubator. (Cylinder and valve are prepared by the users) The cylinder
should be placed near the incubator and be connected with a silicon soft tube to the “CO2
inlet” on the back of the incubator.
3. The ideal ambient temperature is 20～25℃. The lowest ambient temperature is 5℃.
4. The power outlet should be well earthed and be compatible to the incubator plug.
V. Operation procedures.
After installation of the incubator, follow the procedures below to operate.
1. Open the door and clean the inner chamber. Mount the shelves.
2. Fill water to the humidifying to 2/3 level and place it to the bottom of the chamber when
humidifying is needed.
3. Turn on the switch on the back of the incubator. The temperature will be displayed.
4. Press “UV”key to disinfect the chamber. (Do not press it when there’s incubation samples
inside!)
5. Set the required temperature. (Refer to the attached controller manual)
6. When the temperature is stable, put the sample in.
7. Turn on the CO2 cylinder switch and adjust the pressure reducing valve to get 0.06 ～
0.1Mpa on the second pressure manometer. If the CO2 pressure is too high then the rise of
CO2 concentration is too quick and the gas fluctuation will be high too. But if the CO2
pressure is too low it will trigger the alarm. In 1 minute you can see the CO2 concentration
is increasing and in 10 mins it will reach 5.0% (if set to 5.0%).
8. Refer to the controller manual for detailed operation methods.
9. When stop using the incubator please follow the procedures below:
1) Turn off the CO2 Cylinder valve and the pressure reducing valve.
2) Turn off the controller of the incubator.
3) Open the door and get the humidifying plate out. Press the door switch to make it
continue working for several minutes with the door open to dissipate the humidity in the
chamber.
4) Close the door and keep it heating for about 10 minutes then turn off the power switch and
clean the inner chamber.
VI. Controller operating instructions
1. Panel to reference chart (With the CO2 temperature controller)

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2.Panel has a set of buttons on the right，functions as follows:
1)
超 温
电 源 高水位 缺 水
门加热
照 明
灭 菌
气泵1 气泵2
℃
min
水加热 气 泵 2气 泵 1 灭 菌 照 明
button: After power-on, press this button to enter the temperature setting state; Press
this button 5s to enter the other parameters setting state; When the instrument enter the set
state, digital tube bottom flicker.
2) Pump 1: Press this button, the pump 1 through, push once more, the pump 1 disconnects.
3) Pump 2: Press this button, the pump 2 through, push once more, the pump 2 disconnects.
4) Sterilize button: Press this button to open the interior sterilization lamp, push once more,
The sterilization lamp go out.
5) Light button: Press this button to open the light, push once more will turn off the light
6)▲ key: Press this key in the set status to increase the set value.
7)▼ key: Press this key in the set status to decrease the set value.
8) ◄key: Press this key in the set status to shift to required digit which will flicker. In the
state of power-on, press the key, you can view the temperature.
Remark: After setting parameters, only press
超 温
电 源 高水位 缺 水
门加热
照 明
灭 菌
气泵1 气泵2
℃
min
水加热 气 泵 2气 泵 1 灭 菌 照 明
key you can set parameters into the
memory.
3. Operating instructions
1) Runtime, temperature window (top) shows the temperature in the cabinet. Time window
(bottom) shows the remaining time (time setting to 0, window display set temperature),
when the remaining time down to 0, display end, the end of the run. At the end of the run,
click the start running again.
2) Set the temperature in the cabinet, runtime, the steps are as follows: ①press
超 温
电 源 高水位 缺 水
门加热
照 明
灭 菌
气泵1 气泵2
℃
min
水加热 气 泵 2气 泵 1 灭 菌 照 明
button,
the above digital tube display set temperature, the bottom in the flashing state ②Modify
parameters by ▲、▼、◄ key ③And then press
超 温
电 源 高水位 缺 水
门加热
照 明
灭 菌
气泵1 气泵2
℃
min
水加热 气 泵 2气 泵 1 灭 菌 照 明
key, discharge digital tube display
setting time ④Modify parameters by ▲、▼、◄ key ⑤And then press
超 温
电 源 高水位 缺 水
门加热
照 明
灭 菌
气泵1 气泵2
℃
min
水加热 气 泵 2气 泵 1 灭 菌 照 明
key to set
parameters into the memory, exit the set state, returns the running state.
3) Setting the other parameters are as follows: ①press
超 温
电 源 高水位 缺 水
门加热
照 明
灭 菌
气泵1 气泵2
℃
min
水加热 气 泵 2气 泵 1 灭 菌 照 明
button above 5s, enter the
other parameters setting, the above digital tube display each parameter symbols, discharge
digital tube display parameter settings, the bottom in the flashing state ②Modify parameters
by ▲、 ▼、 ◄ key ③And then press
超 温
电 源 高水位 缺 水
门加热
照 明
灭 菌
气泵1 气泵2
℃
min
水加热 气 泵 2气 泵 1 灭 菌 照 明
key, save the current modification parameters, and
adjusted the next parameter, recycle like this ④After setting, press
超 温
电 源 高水位 缺 水
门加热
照 明
灭 菌
气泵1 气泵2
℃
min
水加热 气 泵 2气 泵 1 灭 菌 照 明
button above 5s to
exit the set state, returns the running state.
4) Set the PID parameters as below: ①press
超 温
电 源 高水位 缺 水
门加热
照 明
灭 菌
气泵1 气泵2
℃
min
水加热 气 泵 2气 泵 1 灭 菌 照 明
button above 5s, enter the other
parameters setting, and then press ◄ key above 5s to enter the PID parameters setting, the
above digital tube display set temperature each parameter symbols, discharge digital tube
display PID parameters, the bottom in the flashing state
②Modify parameters by ▲ 、▼、◄ key ③And then press
超 温
电 源 高水位 缺 水
门加热
照 明
灭 菌
气泵1 气泵2
℃
min
水加热 气 泵 2气 泵 1 灭 菌 照 明
key, save the current
modification parameters, and adjusted the next parameter, recycle like this ④After setting,
press
超 温
电 源 高水位 缺 水
门加热
照 明
灭 菌
气泵1 气泵2
℃
min
水加热 气 泵 2气 泵 1 灭 菌 照 明
button above 5s to exit the set state, return the running state.
5) Check the door temperature: in the running state, press ◄ key, temperature window
blinking gate and display door temperature , three digital tube flashing at the same time ,
press ◄ key again, return to display the temperature inside the box.
6) Other parameters' definition and description:

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character
scope
instructions
The factory
setting
AL
0～10.0℃
over temperature alarm
1.0℃
SC
-10.0～10.0℃
Measurement error correcting
random
Td
-10.0～10.0℃
Temperature difference of the oven and
door
random
TS
0～10.0℃
The difference temperature of water
temperature and in the oven
random
TT
0～300S
Closed heating delay time
random
PID parameter symbol definition and description
character
scope
instructions
The factory
setting
P1
0～50.0℃
The temperature in the cabinet control
proportional limit
random
I1
1～2500S
The temperature in the cabinet integration time
random
D1
1～1000S
The differential time of temperature in the
cabinet
random
P2
0～50.0℃
Temperature control door schedule the
proportional limit
random
I2
1～2500S
Door temperature integral time
random
D2
1～1000S
Door temperature differential time
random
4. Wiring diagram
To the display
panel
Transformer
Mainboard
Pump
Pump
Fan
Light
Door
heat
Heat
The UV lamp
Cabinet temperature
Door temperature
Resistance/100Ω
Door switch

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5. Remark:
1）When the box sensor wire break or short circuit, temperature display ER1; Door sensor
wire break or short circuit, temperature display ER2 , Water sensor wire break or short circuit,
temperature display ER3 ,Water level detection error, display ER4,continuous alarm buzzer;
2) Measuring temperature > set temperature + AL, the buzzer alarm intermittently, When
measuring temperature < set temperature + temperature alarm value, the buzzer closed alarm,
press any key cancelable over temperature alarm sound.
Ⅶ. Use
1.Watering: Connect the rubber hose with the inlet and outlet which back of the equipment,
the other end connected to the water, open the inlet-outlet valves. Using water pressure to
force water injection water jacket. When the injected water more than 12L (water level
above the electric heat pipe), can power-on and continue injected water, viewing water
level lamp (may be water shortage alarm at that time ) until the high water level indicator
light green to stop water, close the inlet-outlet valves.
2.In order to prevent the water jacket in scale, please use pure or distilled water when filled.
3. The configuration of the PH liquid and washing liquid
According to the following mixing PH liquid and washing liquid, and inject in PH and
washing bottles respectively.
A. Washing liquid - copper sulfate solution CuSO2 / 5H2O 0.7～
1.4g
H2SO4
0.25ml
Musk toppings (little)
Distilled water 500ml
B. PH liquid - liquid phenol red According to the room temperature ,
changing it about two months
Redistilled water 1000ml
0.2% phenol red 3ml
NaHCO3 following table
Temperature(℃)
14
16
18
20
22
24
26
NaHCO3(g)
1.87
1.80
1.73
1.67
1.61
1.54
1.48
temperature(℃)
28
30
32
34
36
37
38
NaHCO3(g)
1.43
1.38
1.33
1.28
1.23
1.21
1.18
Ⅷ. Use operations sequence
After incubator installing, follow these steps
1. Open the door, and clean indoor, put shelf.
2. Set to 2/3 water in humidity when need, place it at the bottom of studio and then close the
door.
3. Open the power switch which behind the control box, incubator should be displayed
temperature and WH indicator is bright.
4. Push the "UV" button, sterilization for workshop. (Notice: Please do not click this button
when there are incubation samples inside of the chamber!!!) After the sterilization pressing
the "UV" button to close the germicidal lamp.
5. Set the required temperature first, and then heating incubator, (see the five, the controller
instructions)

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6. At constant temperature, open the door and put the culture in.
7. Press “pump1”or “pump2”button to open the pump1 (or2), adjust air flow valve slowly
(counterclockwise), the ratio of CO2 concentration usually 5%, air flow can be adjusted to
760 ml/min.
8. Open the CO2 gas cylinder valves, regulating CO2 PORV, the pressure output is 0.06 Map
(about 0.6 KGF/cm2),open the regulator valve (clockwise) general factory has opened, then
slowly adjust CO2 flow meter to 40 ml/min (counterclockwise). (CO2 concentration ﹦40/
(760 ﹢40) ﹦5% V/V).
9. Once that is done, let the temperature stabilize, the device can develop into automatic
control state.
10. If want to see the washing bottles and PH solution, press "light" key to open the lamp.
11. When the incubator stops working, please follow these steps:
1) Turn off the CO2 cylinders and PORV
2) Shut off the pump power to stop working.
3) Open the door and remove humidity. Open the door and let it work a few minutes to
disperse the moisture in the cabinet.
4) Close door and heat ten minutes, turn off power, clean inside.
Ⅷ.Important information.
1. The equipment should be installed in a clean and temperature-stable place.
2. Read the manual carefully and master the correct using methods before turn the power on.
3. There’s fuse in the equipment. If the equipment is not electrified, please check the
fuse. When checking or changing the fuse, please turn off the power supply. Only same style
fuse is allowed.
4.Cannot power-on when there has not water ,to avoid damage internal parts
5. When there are incubation samples inside of the chamber, do not open the UV light,
otherwise the samples are damaged. Please shut off the power when replace tube.
6. Should be send air first, then CO2, before this, should tune the flow meter knob to the
minimum (clockwise), or it will cause the washing-up liquid and PH liquid out due to the
large flow rate, on the other hand, should close CO2 first, then turn off the air
7. Washing liquid and PH liquid in use will be concentrated and pollution, should be open and
exchange, but must pay attention to, add or change should be cut off the air and CO2 gas
source. Shut off the pump and CO2 pressure reducing valve.
8. Should ordinary observation regulating flow meter is accurate or not, in order to ensure the
stability of the CO2 concentration. (Especially ，pressure and flow will influence each other
at the start-up.
9. CO2 gas cylinder pressure is too low for generate flow instability, should transfer or
inflatable before using
10. In order to save electricity and prolong the life of fluorescent lamp, lighting switch can
switch when need
11. The equipment should be well earthed. Check the earth wire carefully before using.
12. This machine is equipped with air pump to maintain flow accurately, should often adjust
the flow meter. Please shut off the power when you change the pump.
13. In order to reduce the closed form difference, influence the uniformity,
so began to heat when closing the door after 10 min( According to actual usage set delay
time).Please do not open the door regular.
14. please let the water in the jacket go if not need for a long time .

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Ⅸ. Malfunction handling.
Malfunction
Cause
Handling
1.Alarm when
turning on.
Sensor error.
Refer to malfunction 2
Not filled with water
Continue inject water to WH light
2.Errol
display
ER1
Chamber sensor error or
short circuit.
Check the wire or change the sensor.
ER2
Door sensor error or
short circuit.
Check the wire or change the sensor.
ER3
Water sensor error or
short circuit.
Check the wire or change the sensor.
ER4
Water level detection
error
Check the wire or change the sensor.
3. No power
Not plugged or wire
broken.
Plug it or check the wire.
Fuse open circuit.
Change the same type fuse.
4. No heating.
Preset temperature too
low.
Reset the temperature.
Door open or door
switches error.
Close the door or replace the door
switch.
Heater error.
Replace the same type heater.
Temperature control
error
Replace
5.Temperature
demonstration
inaccurate
Is not correct
According to the instruction manual
revised SC value
Ⅹ. Storage and transportation.
The incubator should be stored in well ventilated room with the RH no more than 80% and
without erosive gas. Shockproof, moisture proof and other protection measurements should be
taken during the transportation.
IX. After-sales service.
We guarantee free repairs, changing and returning back in one year period (except for heating
units). In the guarantee time if the equipment is not valid due to quality problems, we will fix
or change parts for free. After one year’s guarantee time, we will try to help with the customer
service first spirit.

Drawell International Technology Limited
Web : www.drawell.com.cn Tel: [phone] Email : [email protected].cn
Packing list
Item
Description
Category
Quantity
Notes
1
CO2Incubator
Main
equipment
1
2
Humidifying plate
Part
1
3
Shelves
Part
2
160L:3
4
Silicon soft tube for CO2
Part
1
5
Fuse
Spare part
1
6
This manual
Document
1
7
Certificate
Document
1
8
Guarantee card
Document
1
The parts listed above are in accordance with the actually packed goods.
Equipment commissioning requirements:
1. Customer-provided 40L CO2cylinder with the CO2concentration of 99.9%.
2. Customer-provided pressure reducing valve (output pressure is about 0.1Mpa).

Drawell International Technology Limited
Web : www.drawell.com.cn Tel: [phone] Email : [email protected].cn
Drawell International Technology Limited
Chongqing Drawell Instrument CO,.Ltd
Add:Suite 2705,Building No.12,Shiyou Road No.1,Yuzhong District,
Chongqing,China
Tel: [phone]
Shanghai Drawell Scientific Instrument Co.,Ltd
Add : Suite 1117,Lane561 XiuChuan Rd.,PuDong New Area,Shanghai,China
Web : www.drawell.com.cn
Email : [email protected]