Ecom ECP2050 User manual

ECP2050
ECOM spol. s r.o., Trebonicka 239, 252 19 Chrastany u Prahy, Czech Republic
tel: [phone], fax: [phone], [email protected], http://www.ecomsro.com/
IČO: 411 92 192, DIČ: CZ411 92 192
User´s Guide
ECP2050
PREPARATIVE HPLC PUMP

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8/5/2021 8:48:00 AM Kateřina Kněžourková
Ver.
Date
Author
Approved by
Description
00
26.06.2019
Kateřina Kněžourková
Jaroslav Formánek
First issue.
00
05.11.2019
Dominika Pavlová
Jaroslav Formánek
Address change in the footage of the first
page, the in chapters 13 and 15.
01
08.04.2020
Dominika Pavlová
Jaroslav Formánek
Declaration of Conformity update in chapter
15.
02
11.12.2020
Kateřina Kněžourková
Jakub Hodaň
Change of port value in chapter 6.4.1.
03
05.08.2021
Kateřina Kněžourková
Jakub Hodaň
Update in chapter 6.4.3.
Related documents
Manual-Ethernet_devices

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CONTENT:
1. GENERAL INFORMATION....................................................................................................6
1.1. Product Function................................................................................................................................6
1.2. Product Version..................................................................................................................................6
1.3. Accessories ........................................................................................................................................6
2. GENERAL OPERATING INSTRUCTIONS ................................................................................8
2.1. Safety Symbols on the Unit...............................................................................................................8
3. DESCRIPTION...................................................................................................................9
4. INSTALLATION AND FIRST START.....................................................................................11
4.1. Inlet Tubing Connection ..................................................................................................................11
4.2. Outlet Capillary Connection ............................................................................................................11
4.3. Injector Holder and Column Holder Mounting...............................................................................12
4.4. Injection Valve Mounting .................................................................................................................12
4.5. Piston Backwash..............................................................................................................................13
4.5.1. Simple Manual Use ....................................................................................................................14
4.5.2. Automatic Backwash..................................................................................................................14
4.6. Assemblage of Waste System ........................................................................................................15
4.7. Connection on Unit Rear Panel.......................................................................................................16
4.8. Start of Unit.......................................................................................................................................17
4.9. Basic Control by Keyboard .............................................................................................................18
4.10. Pump Initiation................................................................................................................................18
4.11. Description of Symbol on the Screen ..........................................................................................19
4.12. Door Installation .............................................................................................................................20
5. CONTROL FROM PC........................................................................................................ 20
6. WORKING WITH MENU ....................................................................................................21
6.1. Parameters........................................................................................................................................21
6.1.1. Flow Rate ...................................................................................................................................21
6.1.2. Pressure Limits...........................................................................................................................21
6.1.3. Concentration.............................................................................................................................22
6.1.4. Solvents Volume ........................................................................................................................22
6.2. Preferences.......................................................................................................................................22
6.2.1. General.......................................................................................................................................22
6.2.2. Purge..........................................................................................................................................23
6.2.3. Gradient......................................................................................................................................23
6.2.4. Compensation ............................................................................................................................24
6.2.5. Solvents......................................................................................................................................25
6.2.6. Log. Level...................................................................................................................................25
6.2.7. Leakage......................................................................................................................................25

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6.3. Control...............................................................................................................................................25
6.3.1. Start Compensation Learning.....................................................................................................26
6.3.2. Gradient Program.......................................................................................................................27
6.3.3. Init/Stop/Reinit Program .............................................................................................................32
6.3.4. Program Setup ...........................................................................................................................32
6.3.5. Open/Close Gradient Valves......................................................................................................39
6.4. IO Config............................................................................................................................................39
6.4.1. Ethernet......................................................................................................................................39
6.4.2. Remote Keylock .........................................................................................................................40
6.4.3. Interface......................................................................................................................................41
6.5. Info.....................................................................................................................................................43
6.5.1. Device.........................................................................................................................................43
6.5.2. Boards........................................................................................................................................43
6.5.3. Ethernet......................................................................................................................................44
6.5.4. User Text....................................................................................................................................44
6.5.5. Pump..........................................................................................................................................44
6.5.6. Parts ...........................................................................................................................................44
6.6. Diagnostics .......................................................................................................................................44
6.6.1. Errors..........................................................................................................................................44
6.6.2. Warnings ....................................................................................................................................45
6.6.3. Power .........................................................................................................................................45
6.6.4. Cooling .......................................................................................................................................45
6.6.5. Interface......................................................................................................................................45
6.6.6. Leakage State ............................................................................................................................45
6.6.7. Pump..........................................................................................................................................46
6.6.8. Solvents......................................................................................................................................46
6.6.9. Pulsation.....................................................................................................................................46
6.6.10. Gradient State ..........................................................................................................................47
6.7. Service...............................................................................................................................................47
6.7.1. Parts Replacement.....................................................................................................................48
6.7.2. Calibrations.................................................................................................................................52
6.7.3. Factory Settings .........................................................................................................................55
6.7.4. Special........................................................................................................................................56
7. WORKING MODES ..........................................................................................................56
8. RS232..........................................................................................................................56
9. REPLACEMENT OF LINE FUSE..........................................................................................57
10. TROUBLESHOOTING......................................................................................................57
11. MAINTENANCE .............................................................................................................60
11.1. Periods.............................................................................................................................................60
11.2. Cleaning and Decontamination.....................................................................................................60

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11.3. Cleaning Check Valves ..................................................................................................................60
11.4. Storage and Transport...................................................................................................................60
12. SPARE PARTS..............................................................................................................61
13. WARRANTY AND POST-WARRANTY REPAIRS..................................................................62
14. TECHNICAL PARAMETERS .............................................................................................63
15. APPENDIX 1–DECLARATION OF COMFORMITY ...............................................................64

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1. GENERAL INFORMATION
This unit is designed for use in laboratories in systems of liquid chromatography as
preparative HPLC pump.
1.1. Product Function
The pump is double-acting with two pumping blocks which are connected in parallel. Both
pumping heads are equipped with inlet and outlet valve. Smooth flow of mobile phase is ensured
by suitable shape of driving cams, achieving steady run of ceramic pistons pumping in back
pressure and also by precise control of motor rotation. The pump works independently as
isocratic. Together with unit Gradient box without degasser ECB2007, ECB2007PC, supplied
separately, works also as a gradient pump. If the box is disconnected, the unit informs about its
disconnection. When entering menu items designated for gradient mode (e.g.
Menu/Parameters/Concentration) and pump is isocratic mode, unit informs too.
1.2. Product Version
ECP2050 Preparative HPLC pump Cat. No.: ACC0000X
1.3. Accessories
BASIC ACCESSORIES
P/N
Qty.
Picture
Description
EKAB-011
1
Mains cable 10A-250V 2 m
00343122
1
Tube fuse T –3,15A/250V, CSA
(main unit fuse; for combined power socket with main switch and main
fuse)
23987000
1
Tube fuse T - 6,3A/250V, CSA
(internal fuse for professional service; mustn’t be used as main fuse
!!!)
EKAB-040
1
LAN cable 1 m
(to connector ETHERNET)
00051478
1
Terminal block 8 pins green
(for connector IO INTERFACE)
18331000
1
Screwdriver Torx T20
(for mounting injector holder, mounting heads during seals
replacement)
E1132890
1
Open End Wrench 1/4-5/16"
(for metal through bolts on capillaries, for mounting valve holders)
PN000080
1
Waste tubing bypass 3/32"
(from by-pass valve to waste system)

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P/N
Qty.
Picture
Description
ACA99012
1
Injector holder Series 2000
ACC99400
1
Capillary injector-column 1mm with fittings
(OD=1/16“ ; ID=1mm ; l=300mm ; volume=236µL ; thread 10-32 UNF)
ACC99300
1
Outlet capillary to injector 1mm with fittings
(OD=1/16“ ; ID=1mm ; l=300mm ; volume=236µL ; thread 10-32 UNF)
ACC16001
1
FEP tubing 1/8“ 1000 mm with filter 20um with lid
(FEP tubing, ID=1/16“, volume=1986 µL only tubing, PEEK screw 1/8“
, KEL-F ferrule 1/8“, thread ¼“-28 UNF, PP lid on tray GL45 with PA
plug with tubing holes, PE filter, PEEK screw 1/8“ , KEL-F ferrule 1/8“)
ACA99700
1
Piston back washing set
ACA99000
1
Waste set ECP2010
00016000
1
Door 2000
(To be installed after complete installation of the unit! Never
manipulate with the unit, if the door is mounted!)
DOC00001
1
-
User’s guide
DOC00002
1
-
Production protocol
Spare parts and selectable accessories see 12.

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2. GENERAL OPERATING INSTRUCTIONS
Caution: If the unit is used in a manner not specified by the manufacturer, the
protection provided by the unit may be impaired!
Caution: The unit may not be used, if it is leaking.
Caution: Do not place unit or any other equipment so that disconnecting power cord
is difficult.
Caution: Never manipulate with the unit, if the door is mounted. There is a danger of
its damage.
Caution: Never dismount unit cover. There is nothing needing customer service or
maintenance.
Caution: The pump is filled with isopropanol after production or service.
Caution: When using buffers or other crystalizing agents in mobile phases, piston
back washing must be done.
Caution: The pump and box must be calibrated together, see Production protocol.
2.1. Safety Symbols on the Unit
Caution, electrical device! Disconnect power cord before servicing.
Electrical device! Disconnect power cord before servicing. Read the
operation guide before replacing fuse!

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3. DESCRIPTION
FRONT VIEW
No.
Description
1
Front switch.
2
Keyboard.
3a
Connection for input tubing (pump inlet) –working block with pumping and washing
head (behind the pumping head).
3b
Connection for output capillary (pump outlet).
4
Washing/degassing (bypass) valve –compensating block with pumping and washing
head.
5
Leakage sensor.
6
Drain channel with cone for connecting waste system.
7
Door hinges.
8
Slots for tubing and capillaries.
9
Display.
10
Status LED:
PUMP (green) for indication of pump motor status (PUMP and PURGE).
RMT (yellow) for indication of unit remote control status. If it is on, the unit
communicates through ETHERNET or RS232 interface.
ERR (red) for indication of unit error status. If it is on, there is an error indicated, if it
blinks, there is a fatal error indicated and unit status has changed.

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KEYBOARD BUTTONS AND THEIR FUNCTION
Symbol
Description
MENU
Entering unit menu, fast menu leaving.
PUMP
Pump start/stop and other functions when working with gradient program
(see 6.3.2).
FLOW
Direct setting of pump flowrate.
PURGE
Entering purge mode.
ENTER
Confirms selected MENU item, confirms newly set parameter value,
evokes QUICK DIAGNOSTICS screen.
ESC
Returns one step back without saving changes.
“B“, “ D“, “
A“, “C“
Direction keys.
Using arrows up/down increases/decreases value of set parameter.
A, B, C, D –direct choice of mobile phase valve.
D –starts gradient program.
MENU
Entering unit menu, fast menu leaving.
REAR VIEW
No.
Description
1
Combined power socket with main switch and main fuse.
2
Connector for adding box.
3
Connector for external start.
4
Connector IO Interface.
5
Connector Ethernet/LAN.
6
Connector USB.
7
Connector RS232.

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4. INSTALLATION AND FIRST START
Place the unit in a suitable location that satisfies the following conditions:
•Horizontal place.
•Keep at least 10 cm space behind the rear of the unit.
•Keep away from equipment generating strong magnetic field.
•Equipment is intended for use in regular laboratory environment only - see Technical
parameters –operating environment conditions.
4.1. Inlet Tubing Connection
For connecting pump in isocratic mode, use inlet tubing with lid, filter, screw and ferrule
(ACC16001) from supplied accessories. For gradient mode connection is supplied inlet tubing
(ABD21000), which is among accessories of the box.
At first unscrew the white cap on pump inlet.
Caution: The pump is filled with isopropanol after construction or service.
Isocratic connection of pump
inlet
Connect inlet tubing with filter.
Snap the tubing to the socket for
tubing and capillaries.
Set of inlet tubing with a lid and
filter (picture on the right).
Gradient connection of pump
inlet
Connect inlet tubing. Connect the
other end to the box.
Tubing is fixed into the upper
socket for tubing and capillaries.
Note: The upper and side sockets in the unit front panel are determined for gripping
tubing and capillaries. FEP tubing with outer diameter 1/8“ are fixed by snapping
into the slot. Capillaries and tubing with outer diameter 1/16“ are fixed using a
short FEP tubing with outer diameter 1/8“ and inner diameter 1/16“, threaded on
the capillary. Such a threaded tubing cannot be snapped in the slot, it should be
pushed in from the side.
4.2. Outlet Capillary Connection
To connect the pump to injector, use outlet capillary (ACC99300). For connecting pump to
column, use outlet capillary (ACC99400).

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Connect outlet capillary.
Tighten capillary nut with side
wrench 1/4"
Connect the other end of capillary
to injector valve.
4.3. Injector Holder and Column Holder Mounting
Use Torx screwdriver TX20 to
unscrew two screws in front part of
the right panel of the unit.
Use the unscrewed screws to
mount the injector holder,
according to the picture.
4.4. Injection Valve Mounting
Injection valve is not a part of pump accessories, neither a part of the entire system
accessories and shall be ordered separately.
The injection valve holder is constructed for mounting two different types of valves and with
hole for outlet capillary from pump to injection valve –Rheodyne and Vici type. The difference is in
spacing of holes for mounting the valve, see picture below.
Spacing 25.4 mm is designed for injection valve
Rheodyne.
Spacing 27.5 mm is designed for injection valve
Vici.
Spacing 12.5 mm is designed for outlet capillary
from pump to injection valve.
All diameters are in millimeters (mm).
Injection valve Rheodyne is
mounted to the holder with two
screws using allen/imbus key.
Usually it is among the injection
valve accessories.

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Handle of injection valve is
mounted with two imbus worms,
using imbus key 2 mm.
The cable is connected to
connector START on unit rear
panel.
Injection valve Vici is mounted to
the holder with two screws
M4 x 6 mm instead of original
M4 x 10 mm, using imbus key
3 mm. Screws and imbus key are
among the injection valve
accessories if it is ordered in
Ecom spol. s r.o.
Handle of injection valve is
mounted by snapping into the
valve body.
Put the cable through the window
and connect to connector START
or IO Interface connector on unit
rear panel. Make configuration
(see. 6.4.3).
4.5. Piston Backwash
Caution: If you are using puffers or other crystallizing agents in mobile phases, you
must use piston backwashing.
Each pumping block (left and right) has two heads –pumping and washing. Pumping heads
are farther from the unit. Washing heads have in upper and lower part slot with LUER cone, for
connection of piston backwash. Both heads are equipped with a seal (piston padding) and during
work with buffer solutions may buffer crystals adhering to the piston may damage the seal and
pump can start leaking.

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SET PISTON BACKWASH
1. Inlet part; 1a. Luer cone for connecting to washing head; 1b. Flowrate regulator; 1c. Lid GL45 with plug;
1d. Luer cone for connecting syringe; 2. Connection of washing heads; 3. Outlet into waste system;
4. Syringe 50ml
4.5.1. Simple Manual Use
Connect both Washing heads in series using tubing and LUER adapters. Fill syringe with
HPLC water (or mobile phase dissolving buffers) and wash the heads. Washing heads are now
filled with liquid, rinsing the pistons from rear side. Now once in a while during work with the pump
push through the pump some liquid to the washing system (approx. 1x per hour 5 ml).
4.5.2. Automatic Backwash
Connect both Washing heads in series using tubing and LUER adapters. Insert tubing with
flow regulator into reservoir with HPLC water (or mobile phase dissolving buffers). Loosen the flow
regulator and suck liquid into the system using plastic syringe connected to the other end of the
system (left picture). Disconnect the syringe and place the tubing end into waste reservoir. Now the
liquid is flowing through the system by gravity (right picture). Set the flow regulator so that at the
end of the tubing escapes approx. 5-7 drops per minute.

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4.6. Assemblage of Waste System
he pump is equipped with waste channel, leading the liquid off the pumping block. In its
proximity is leakage sensor. The function of the sensor is closely described in chapter 6.2.7, in
chapter 6.4.3.4 is described the setting of IO INTERFACE Digital Out Switch –LEAKAGE
signalizing the leakage of pump to another unit. In chapter 6.6.5 is displayed the status of IO
INTERFACE and in chapter 6.6.6 is shown the leakage sensor status.
WASTE SET ECP2050
1. Silicone tubing 2 m with connector Y and I; 2. Connector Y with two pieces of silicone tubing;
3. Connectors L into the drain channel.
The pump is usually a part of a system, together with other units. Connection of waste
system can be adjusted according to given system.
One possible system is pump with box. Among box accessories is T connector, one end
should be pushed into the box drain channel (see picture below). Second end should be connected
with longer tubing of part 2 (see picture above) and third end may be used i.e. for waste tubing
from injection valve.

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Shorter tubing of part 2 (picture above) should
be connected between the pump and the backstop
on holder of injection valve. This tubing serves as
waste tubing from washing head, put through window
in valve holder. The opening of part 2 should be
connected with connector L, forming a 90° angel with
desktop, and is connected with one of two tubing,
connected to connector Y of part 1. This tubing
should be cut from 1 m silicon tubing part 1,
according to your needs. Second connector L is
pushed into the drain channel of pump, horizontally,
and is connected with second tubing of connector Y
of part 1. Third tubing of part 1 should be placed into
waste reservoir. Connector Y of part 1 is placed
under level of the working surface.
Caution: Replace the connector Y of part 1 at least 10 cm below the desktop.
4.7. Connection on Unit Rear Panel
Plug the power supply cord into the instrument socket on unit rear
panel. The cord is among unit accessories.
Plug the interconnecting cable between pump and box into
connector ECB2000. Cable is among box accessories.

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Plug injection valve cable into START connector. Plug it all the
way in until it snaps in.
In order to unplug the connector, press the safety pin (see arrow
on right picture) and pull out.
Insert terminal block into connector INTERFACE, it is among the
pump accessories. If there is no signal connected to the terminal
block, it is not necessary to connect it.
Plug LAN network cable into connector ETHERNET, it is among
the pump accessories. Press the connector in until the safety pin
snaps in. In order to unplug the connector, press the safety pin
(see arrow on the right picture) and pull out.
The other end of network cable shall be plugged into PC or
computer network element.
Connector USB serves for plugging USB cable for interconnection
with PC.
Cable is not among the unit accessories.
Plug serial line cable into connector RS232 for interconnection with
PC.
Cable is not among the unit accessories.
4.8. Start of Unit
Use a switch on the rear panel of the unit for connecting to mains voltage, then switch on the
unit using push button on the front panel. The push button glows red, if power source is off and
green, if power source is on. If power supply is disconnected, the unit remembers last status of this
push button and after power supply reconnection it restores the last status. This can be used for
automatic start in the system with a common switch of power source for whole system of liquid
chromatograph (for example using a multiple socket-outlet with a switch).

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4.9. Basic Control by Keyboard
After unit start (mode START) display shows unit
type.
Then the unit turns to mode IDD, pump motor is in
idle status.
Main screen –isocratic pump mode.
Main screen –gradient pump mode.
Gradient mode is detected automatically by cable
interconnection with a box after unit start.
4.10. Pump Initiation
The unit is filled with isopropanol, it is necessary to rinse it out and fill the system with
mobile phase and degas the entire system.
Loosen purge/degassing valve by turning it by
90°.
Slip a plastic syringe on purge/degassing valve,
the syringe (50ml) is a part of pump accessories.
In gradient mode is necessary to check, whether appropriate valve (A, B, C or D) with mobile
phase is opened. By pressing key PURGE and then pressing key PUMP is started pump washing.
Suck in the mobile phase with the plastic syringe as long as bubbles come out from the pump. It is
possible to switch the valves during washing, by pressing keys with arrows, this allows the liquid to
penetrate to all necessary channels Press key PUMP again to stop the pump washing. Tighten the
purge/degas valve and take out plastic syringe.
For repeated gas purging/degassing it is possible
to put the waste tubing on purge valve.
If waste tubing is put on purge/degas valve, it is
not possible to close the unit door.
In chapter 6.2.2 it is possible to set parameters
foe washing.
Now the flow can be set by pressing key FLOW.
Use arrows left and right to move with cursor and
arrows up and down to change value. Press
ENTER key to confirm the value, press PUMP to
start the pump. Press PUMP again to stop

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the pump.
For fast setting of one of the valves to 100%, it is
possible to use arrow with the letter of the
appropriate valve. this switching is functional in
gradient mode during modes IDD, PRG a PMP
(see 7).
Note: We recommend to run and stabilize the unit before starting analyzes or
after a longer shutdown of the device at 1 ml/min, pressure 15-20 MPa for
approximately 30 min.
Note: Run the gradient pump with a 25 % concentration setting for each valve
approximately 10 min.
4.11. Description of Symbol on the Screen
Value/Symbol
Meaning
10%20%30%40%
0 ml
Actual gradient concentration for A, B, C, D.
In isocratic mode is displayed remaining mobile phase volume.
12.0-15.0
Value of low –high pressure limit in actually set pressure units.
1.00ml/min
Actual flowrate value.
13.0MPa
Actual pressure value.
IDD
Abbreviation of actual pump mode.
Actual piston position is displayed after first finished pump rotation.
During flowrate higher than ~3 ml/min actual position cannot be displayed
and this dummy symbol is shown.
Pulsation compensation status:
OFF –turned off.
FIXED –according to predefined values.
LEARNING –learning in progress.
LEARNED –according to learned values.
LEARNED OFF –compensation is turned off.
LEARNED 0 –according to learned values (from FW 5.x).
LEARNED 0 –compensation is turned off or not learned (from FW 5.x).
LEARNED 1 –according to learned values (from FW 5.x).
LEARNED 1 –compensation is turned off or not learned (from FW 5.x).
/ / / /
Gradient valve status (A, B, C, D). Only in gradient mode. During very fast
valve switching (low percentage of component and higher flowrate) it may
not manage to display valve switching.
Mark X –all valves are closed.

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Value/Symbol
Meaning
Indication of leakage, at the same time may be displayed an error or
warning (according to setting).
One IO INTERFACE inputs are used for pump control.
E(1)
An error has occurred „ERROR“ (number of errors). By using key ENTER
on main screen, it is possible to enter screen QUICK DIAGNOSTICS and
see a description of all errors and warnings.
W(1)
„WARNING“ (number of warnings). By using key ENTER on main screen, it
is possible to enter screen QUICK DIAGNOSTICS and see a description
of all errors and warnings.
4.12. Door Installation
In order to prevent damage of door hinges, the door is supplied apart. The door should be
slipped on the hinges in opened position, see pictures below.
Door mounting must be easy, no strength needed, otherwise the hinges may get damaged.
Caution: Never manipulate with the unit when the door is mounted.
5. CONTROL FROM PC
During unit control from PC communication proceeds through serial line RS232, USB or
through LAN. Communication protocol can be supplied upon request.
Unit may be controlled with SW Clarity or ECOMAC.
When using program Clarity for pump control, use last upgraded program version, including
drivers for this unit. Help regarding the control of this unit can be found in installation directory of
Clarity (C:\Clarity; C:\Clarity\Bin) in file: CswEcomECP2000.chm . Double-click on the file opens it
for reading.