Esco Medical Mini MIRI Dry / Humidity User manual

Rev. 1.0
Date revised 01.11.2021
Rx only
Mini MIRI® Dry / Humidity Multiroom
incubators
CE 0088

Mini MIRI® Dry/Humidity incubators User Manual Rev. 1.0
2
Esco Medical Technologies, Ltd.
Draugystės street 19 • Kaunas, Lithuania
Tel [phone]
medical.escoglobal.com •[e-mail]
For Technical Service, contact
North America
Esco Technologies, Inc.
903 Sheehy Drive, Suite F, Horsham, PA 19044, USA
Tel [phone] •Fax [phone]
www.escolifesciences.us •[email protected]
Rest of the World
Esco Micro Pte. Ltd.
21 Changi South Street 1 • Singapore 486 777
Tel [phone] •Fax [phone]
www.escoglobal.com • [email protected]
Copyright Information
©Copyright 2014 Esco Micro Pte Ltd. All rights reserved.
The information in this manual and the accompanying product is copyrighted and all
rights reserved by Esco.
Esco reserves the right to make periodic minor design changes without obligation to no-
tify any person or entity of such change.
Sentinel™ is a registered trademark of Esco.
Caution: Federal law restricts this device to sale by or on a licensed healthcare practition-
er's order.
Only to be used by a trained and qualified professional. The device is sold under exemp-
tion 21 CFR 801 Subpart D.
“Material in this manual is provided for informational purposes only. The contents and the
product described in this manual (including any appendix, addendum, attachment, or
inclusion) are subject to change without notice. Esco makes no representations or
warranties as to the accuracy of the information contained in this manual. In no event shall
Esco be held liable for any damages, direct or consequential, arising out of or related to the
use of this manual.”

Mini MIRI® Dry/Humidity incubators User Manual Rev. 1.0
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Unpacking and Inspection
Follow standard receiving practices upon receipt of the instrument. Check the shipping
carton for damage. If the damage is found, stop unpacking the instrument. Notify the
freight carrier and ask for an agent to be present while the instrument is unpacked. There
are no special unpacking instructions, but be careful not to damage the instrument when
unpacking it. Inspect the instrument for physical damage such as bent or broken parts,
dents, or scratches.
Claims
Our routine method of shipment is via common carrier. If physical damage is found, upon
delivery, retain all packing materials in their original condition and immediately contact
the carrier to file a claim.
If the instrument is delivered in good physical condition but does not operate within spec-
ifications, or if there are any other problems not caused by shipping damage, please con-
tact your local sales representative or Esco Medical immediately.
Standard Terms and Conditions
Refunds & Credits
Please note only serialized products (products labeled with a distinct serial number) and
accessories are eligible for a partial refund and/or credit. Non-serialized parts and acces-
sory items (cables, carrying cases, auxiliary modules, etc.) are not eligible for return or
refund. In order to receive a partial refund/credit, the product must not have been dam-
aged. It must be returned complete (meaning all manuals, cables, accessories, etc.) within
30 days of original purchase, in “as new”and resalable condition. The Return Procedure
must be followed.
Return Procedure
Every product returned for refund/credit must be accompanied by a Return Material Au-
thorization (RMA) number, obtained from Esco Medical Customer Service. All items being
returned must be sent prepaid (freight, duty, brokerage and taxes) to our factory location.
Restocking Charges
Products returned within 30 days of original purchase are subject to a minimum restock-
ing fee of 20% of the list price. Additional charges for damage and/or missing parts and
accessories will be applied to all returns. Products that are not in “as new”and resalable
condition are not eligible for credit return and will be returned to the customer at their
own expense.
Certification
This instrument has been thoroughly tested/inspected and found to meet Esco Medical’s
manufacturing specifications when shipped from the factory. Calibration measurements
and testing are traceable and done according to Esco Medicals ISO certification.
Warranty and Product Support
Esco Medical warrants this instrument to be free from defects in materials and workman-
ship under regular use and service for two (2) years from the original purchase date. The
provided instrument is calibrated and maintained following this manual. During the war-
ranty period, Esco Medical will, at our option, either repair or replace a product that
proves to be defective at no charge, provided you return the product (shipping, duty,

Mini MIRI® Dry/Humidity incubators User Manual Rev. 1.0
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brokerage and taxes prepaid) to Esco Medical. Any transportation charges incurred are
the purchaser's responsibility and are not included within this warranty. This warranty
extends only to the original purchaser. It does not cover damage from abuse, neglect, ac-
cident or misuse, or as the result of service or modification by parties other than Esco
Medical.
IN NO EVENT SHALL ESCO MEDICAL LTD. BE LIABLE FOR CONSEQUENTIAL DAMAGES.
No warranty shall apply when any of the following causes damage:
•Power failure, surges, or spikes
•Damage in transit or when moving the instrument
•An improper power supply such as low voltage, incorrect voltage, defective wiring
or inadequate fuses
•Accident, alteration, abuse or misuse of the instrument
•Fire, water damage, theft, war, riot, hostility, acts of God such as hurricanes, floods,
etc.
Only serialized products (those items bearing a distinct serial number tag) and their ac-
cessory items are covered under this warranty.
PHYSICAL DAMAGE CAUSED BY MISUSE OR PHYSICAL ABUSE IS NOT COVERED UNDER
THE WARRANTY. Items such as cables and non-serialized modules are not covered under
this warranty.
This warranty gives you specific legal rights and you may have other rights, which vary
from province to province, state to state, or country to country. This warranty is limited
to repairing the instrument per Esco Medical's specifications.
When you return an instrument to Esco Medical for service, repair or calibration, we rec-
ommend shipment using the original shipping foam and container. If the original packing
materials are not available, we recommend the following guide for repackaging
•Use a double-walled carton of sufficient strength for the weight being shipped
•Use heavy paper or cardboard to protect all instrument surfaces. Use non-abrasive
material around all projecting parts
•Use at least four inches of tightly packed, industrial-approved, shock-absorbent
material all around the instrument
Esco Medical will not be responsible for lost shipments or instruments received in dam-
aged condition due to improper packaging or handling. All warranty claim shipments
must be made on a prepaid basis (freight, duty, brokerage, and taxes). No returns will be
accepted without a Return Materials Authorization (“RMA”) number. Please contact Esco
Medical to obtain an RMA number and receive help with shipping/customs documenta-
tion.

Mini MIRI® Dry/Humidity incubators User Manual Rev. 1.0
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Re-calibration of instruments, which have a recommended annual calibration frequency,
is not covered under warranty.
Warranty Disclaimer
If your instrument is serviced and/or calibrated by someone other than Esco Medical Ltd.
and their representatives, please be advised that the original warranty covering your
product becomes void when the tamper-resistant Quality Seal is removed or broken with-
out proper factory authorization.
In all cases, breaking the tamper-resistant Quality Seal should be avoided at all cost, as
this seal is key to your original instrument warranty. In an event where the seal must be
broken to gain internal access to the instrument, you must first contact Esco Medical Ltd.
You will be required to provide us with the serial number for your instrument, as well as
a valid reason for breaking the Quality Seal. You should break this seal only after you have
received factory authorization. Do not break the Quality Seal before you have contacted
us! Following these steps will help ensure that you will retain the original warranty on
your instrument without interruption.
WARNING
Unauthorized user modifications or applications beyond the published specifications
may result in an electrical shock hazard or improper operation. Esco Medical will not be
responsible for any injury sustained due to unauthorized equipment modifications.
ESCO MEDICAL LTD. DISCLAIMS ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED,
INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR
PURPOSE OR APPLICATION.
THIS PRODUCT CONTAINS NO USER-SERVICEABLE COMPONENTS.
UNAUTHORIZED REMOVAL OF THE INSTRUMENT COVER SHALL VOID THIS AND ALL
OTHER EXPRESSED OR IMPLIED WARRANTIES.

Mini MIRI® Dry/Humidity incubators User Manual Rev. 1.0
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Table of contents
1 How to use this manual............................................................................................................................ 9
2 Safety warning............................................................................................................................................. 9
3 Indication for use........................................................................................................................................ 9
4 About the product ....................................................................................................................................10
5 Accessories supplied...............................................................................................................................12
6 Safety symbols and labels .....................................................................................................................12
7 Important safety instructions and warnings.................................................................................14
7.1 Before installation ...........................................................................................................................14
7.2 During installation...........................................................................................................................14
7.3 Post-installation ...............................................................................................................................15
8 Getting started...........................................................................................................................................15
9 Mains connection .....................................................................................................................................16
10 Gas connections......................................................................................................................................16
11 HEPA/VOC filter (not available in the Mini MIRI®Humidity) .............................................18
11.1 Installation of new filter capsule (only in the Mini MIRI®Dry incubator) .............18
12 User interface..........................................................................................................................................19
12.1 Activating the heat and gas controls......................................................................................20
12.2 System menu...................................................................................................................................20
12.3 Status..................................................................................................................................................21
12.4 Main menu .......................................................................................................................................21
12.4.1 Temperature sub-menu .....................................................................................................22
12.4.2 CO2 sub-menu .........................................................................................................................23
12.4.3 O2 sub-menu............................................................................................................................25
12.4.4 UV-C light sub-menu (the functionality is not available in the US):..................26
12.4.5 Service sub-menu..................................................................................................................27
13 Installation with premixed gas.........................................................................................................28
13.1 Installation procedure at the site............................................................................................28
13.2 User training....................................................................................................................................30
14 Alarms........................................................................................................................................................31
14.1 Temperature alarms ....................................................................................................................31

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14.2 Gas level alarms .............................................................................................................................32
14.2.1 CO2alarms ...............................................................................................................................32
14.2.2 O2alarms..................................................................................................................................32
14.3 Gas pressure alarms.....................................................................................................................33
14.3.1 CO2pressure alarm ..............................................................................................................33
14.3.2 N2pressure alarm.................................................................................................................33
14.4 Multiple alarms ..............................................................................................................................34
14.5 Alarm UV-C light (the functionality is not available in the US) ...................................34
14.6 Loss of power alarm.....................................................................................................................34
15 Changing the set points .......................................................................................................................35
15.1 The temperature setpoint..........................................................................................................35
15.2 CO2gas concentration set point...............................................................................................35
15.3 O2gas concentration set point .................................................................................................36
15.4 The culture mode ..........................................................................................................................36
16 Surface temperatures and measuring temperature.................................................................37
17 Pressure.....................................................................................................................................................39
17.1 CO2gas pressure............................................................................................................................39
17.2 N2gas pressure ..............................................................................................................................39
18 Firmware...................................................................................................................................................40
19 Safe sense function................................................................................................................................40
20 Cleaning instructions ...........................................................................................................................41
20.1 Considerations about a sterile device ...................................................................................41
20.2 Manufacturer recommended cleaning procedure............................................................41
20.3 Manufacturer recommended disinfection procedure.....................................................42
21 Heat optimization plates.....................................................................................................................42
22 Humidification ........................................................................................................................................43
22.1 The Mini MIRI®Humidity incubator......................................................................................43
22.2 The Mini MIRI®Dry incubator .................................................................................................43
23 Temperature validation ......................................................................................................................44
24 Gas level validation ...............................................................................................................................44
25 Alarm switch for an external system .............................................................................................45
26 Writing area on the compartment lids..........................................................................................46
27 Maintenance ............................................................................................................................................47

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28 Emergency Procedures........................................................................................................................47
29 User Troubleshooting ..........................................................................................................................49
30 Specifications...........................................................................................................................................51
31 Electromagnetic compatibility .........................................................................................................52

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1 How to use this manual
The manual is designed to be read by sections and not ideally from cover to cover. It
means that if the manual is read from start to finish, there will be some repetition and
overlap. We recommend the following method for going through the manual: first, famil-
iarize yourself with the safety instructions; then, proceed to the essential user functions
needed for operating the equipment on a day-to-day basis; then, review the alarm func-
tions. The menu functions of the user interface detail information that is required only
for the advanced users. All parts must be read before the device is taken into use.
2 Safety warning
•Anyone working with, on or around this equipment should read this manual. Fail-
ure to read, understand and follow the instructions given in this documentation
may result in damage to the unit, injury to operating personnel, and/or poor
equipment performance
•Any internal adjustment, modification or maintenance to this equipment must be
undertaken by qualified service personnel
•If the equipment must be relocated, make sure it is appropriately fixed on a sup-
port stand or base, and move on a flat surface. When necessary, move the equip-
ment and the support stand/base separately
•The use of any hazardous materials in this equipment must be monitored by an
industrial hygienist, safety officer or other suitably qualified individuals.
Before you proceed, you should thoroughly understand the installation proce-
dures and take note of the environmental/electrical requirements
•In this manual, important safety-related points will be marked with the following
symbols:
NOTE
It is used to direct attention to a specific item.
WARNING
Use caution.
•If the equipment is used in a manner not specified by this manual, the protection
provided by this equipment may be impaired.
3 Indication for use
The Esco Medical Mini MIRI®incubators are intended to be used to provide a stable cul-
ture environment at or near body temperature and CO2/N2or premixed gases and hu-
midification (in Mini MIRI®Humidity) for the development of gametes and embryos dur-
ing in vitro fertilization (IVF) / assisted reproduction technology (ART) treatments.

Mini MIRI® Dry/Humidity incubators User Manual Rev. 1.0
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4 About the product
Esco Medical Mini MIRI®Dry and Mini MIRI®Humidity incubators are multi-room
CO2/O2incubators.
Direct warming of the dishes in the chambers gives superior temperature conditions in
comparison to conventional incubators.
The compartment's temperature will remain stable up to 1 °C (even when a lid is open
for the 30s) and recover within 1 min after it is closed.
The Esco Medical Mini MIRI® incubators have 2 completely separate culture heat cham-
bers. Each chamber has its heated lid and warming plate for the Petri dish. Mini MIRI®
Dry and Mini MIRI®Humidity capacity for 35mm Petri dish are 16 pcs and 60mm and 4-
well Petri dishes –8 pcs.
To ensure maximum performance, the system of Mini MIRI®Dry and Mini MIRI®Humid-
ity has 4 completely separate PID temperature controllers. They control and regulate the
temperature in culture chambers and lids. Compartments do not affect each other`s tem-
peratures in any way. The top and the bottom of each compartment are separated with a
PET layer so that the lid temperature would not affect the bottom. For validation pur-
poses, each compartment has a PT-1000 sensor built-in. The circuitry is separated from
the unit`s electronics, so it remains a genuinely separate validation system.
The incubators have to be supplied with 100% CO2and 100% N2or premixed gas (for
instance, 5% CO2; 5% O2and 90% N2) to control the CO2and O2concentrations the cul-
ture chambers.
A dual-beam infra-red CO2sensor with extremely low drift rates controls the CO2level. A
chemical, medical-grade oxygen sensor controls the level of O2.
Gas recovery time is less than 3 min after opening the lid. Mini MIRI®Dry and Mini MIRI®
Humidity incubators are fitted with two gas sample ports that allow the user to validate
gas concentration by sampling gas from the individual compartment.
The incubator features a recirculated gas system where gas is continuously put into the
compartment and taken out at the same rate. Gas is cleaned via 254 nm UVC light with
direct gas contact between the bulb and gas, then through a VOC filter and a HEPA filter.
The UVC light has filters that inhibit any 185 nm radiation that would produce dangerous
ozone. The VOC filter is located under the UVC light.
UVC light modules and HEPA-VOC filters are not applied on Mini MIRI®Humidity.

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Complete gas repletion in the system takes less than 5 min.
The total gas consumption is very low. Less than 2 l/h CO2and 5 l/h N2in use.
For safety reasons, the incubator has a complete gas control system that consists of a
pressure regulator (preventing dangerous gas pressure problems), gas flow sensors (ac-
tual consumption can be accumulated), gas pressure sensors (then the user knows that
the pressure and variation can be logged to avoid dangerous conditions), gas filters (to
prevent valve problems).
Petri dish location in a compartment is easy to reach and safe because of the compart-
ment numbering and the ability to write on the white lid with a pen.
The incubator has been primarily developed and designed to incubate gametes and em-
bryos with an overlay of either Paraffin or mineral oil.
In the case of Mini MIRI®Dry and Mini MIRI®Humidity, the upright LED display is large,
clear and easy to read from a distance. The user can tell if the parameters are correct
without going near the unit.
A pH sensor port is part of the DAQ package. The user can plug any standard BNC pH
probe into the unit and measure the pH in the samples at will.
If an open culture (any type of culture where the culture media is not covered with a layer
of oil) is used, the user must switch the device to open culture mode.
Refer to section “15.4 The culture mode”form more detailed information.
The incubator can be connected to a PC running the Esco Medical data logging software
for long term data logging and data storage.
The devices are manufactured under a full EU certified 13485 ISO quality management
system.
This product fulfills the requirements of EN6060-1 3rd edition standards as a Class I
equivalent device suited for continuous operation. It also conforms to the EU Council di-
rective's 93/42/EEC requirements concerning medical devices and is classified as a Class
IIa device under rule II.
Personnel Protective Equipment (89/686/EEC) and Machine Directive (2006/42/EC) is
not applicable for Mini MIRI®incubators.

Mini MIRI® Dry/Humidity incubators User Manual Rev. 1.0
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1.
2.
3.
4.
5.
6.
7.
8.
3.
2.
1.
5 Accessories supplied
•1 VOC/HEPA filter capsule (not available in the Mini MIRI® Humidity incubator)
•2 HEPA filter for input gas supply
•2 warming blocks
•1 USB stick containing Esco Medical Data logger software and PDF versions of the
manuals
•1 medical grade power cord
•4 warranty labels
•1 pump box calibration tool (not available in the Mini MIRI®Dry incubator)
•1 3,5 mm external alarm jack connector
•1 humidity bottle (not available in the Mini MIRI®Dry incubator)
•1 set of fast male connectors with 15 silicone pipes
6 Safety symbols and labels
Several user labels on Mini MIRI®Dry and Mini MIRI®Humidity incubators surface to
guide the user. User labels are shown below.
Table 6.1 Labels
Description
Image
Packing box label:
1. If stored longer than the shelf life, the unit must be
returned to the manufacturer for a new release test.
2. Shipping temperature between -20 °C and +50 °C.
3. Keep away from direct sunlight.
4. Caution: consult the accompanying documents for
important safety-related information such as warn-
ings and precautions that cannot be presented on the
device itself for various reasons.
5. Consult instructions for proper use of the device
6. Do not use it if the packing material is damaged.
7. Rx Only.
8. Keep dry.
1. Consult instruction for use.
2. Warning on the back of the device indicates that an
earth connection is needed and the mains information
and an “ON/OFF” push button.
3. “Lightning bolt”indicates the potential risk of elec-
trical shock (never remove any cover).

Mini MIRI® Dry/Humidity incubators User Manual Rev. 1.0
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1. Model.
2. Mains power rating.
3. CE mark.
4. Not protected against the ingress of water.
5. Manufacturer’s address and country of origin.
6. Consult instruction for use.
7. Observe WEEE.
8. Upper limit of temperature.
9. Keep away from direct sunlight.
10. Keep dry.
11. Logo and serial number.
12. Year of manufacture.
13. Rx only.
Table 6.2 Labels on the back of Mini MIRI®incubators
Description
Image
USB communication port
CO2inlet
N2inlet
Alarm port
Compartments numbers are indicated in the
top corner of the lid with a label
Humidification bottle1
1 Not available in the Mini MIRI® Dry

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Compartment numbers are shown in the picture below and also indicated on the top of
the lid with labels:
Figure 6.1 Compartment numbers
7 Important safety instructions and warnings
7.1 Before installation
1. Do not use the product if the package is damaged. Contact Esco Medical or the local
representative.
2. Read the user manual thoroughly before use.
3. Always keep these instructions easily accessible near the device.
7.2 During installation
1. Never place this unit on top of other equipment that might heat it.
2. Place this unit on a flat, hard and stable surface.
3. Never place the unit on a carpet or similar surfaces.
4. Do not defeat the safety purpose of the grounding-type (earthing) plug.
5. A grounding-type (earthing) plug has two blades and a third prong is provided for
your safety. If the provided plug does not fit into your outlet, consult an electrician
to replace the outlet.
6. Always connect the power cord to a properly grounded outlet and only use the
cord that came with the device.
7. Do not install the device near any heat sources such as radiators, heat registers,
stoves or other apparatus that produce heat.
8. Do not use this device near water sources.
9. Use only 100% concentration CO2and 100% concentration N2or premixed gases.
10. Always use an external HEPA filter for input CO2/N2or premixed gas.
11. Do not use this product if the room temperature exceeds 30 °C.
12. Place this unit in a location with adequate ventilation to prevent internal heat
build-up. Leave at least 10 cm clearance from the rear, 30 cm from the top and 20
cm from left and right to prevent overheating and allow access to the ON/OFF
switch in the back.

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13. This unit is intended for indoor purposes only.
14. The unit must be connected to a suitable uninterrupted power supply (UPS)
source.
7.3 Post-installation
1. Refer all servicing to qualified service personnel.
2. Servicing is required according to the service manual as well as in cases when the
device has been damaged in any way, e. g. suppose the apparatus has been
dropped, exposed to rain or moisture or does not operate normally. Mini MIRI®
Dry and Mini MIRI®Humidity incubators contain high voltage components that
may be hazardous.
3. Unplug this apparatus during lightning storms or when unused for an extended
period of time.
4. Protect the power cord from being walked on or pinched, particularly at the plug,
convenience receptacles, and the point where it exits from the apparatus.
5. Perform temperature and gas calibration at the intervals described in the manu-
als.
6. Never leave the lids of the compartment open for more than 10 sec while in use.
7. VOC/HEPA filters must be changed every 3 months (no change is required in the
Mini MIRI®Humidity multiroom incubator).
8. A maintenance plan must be fulfilled to keep the device safe.
9. NEVER block gas supply holes in the compartment.
10. Ensure that CO2/N2or premixed gas supply pressures are kept stable at 0.4 –0.6
bar (5.80 –8.70 PSI).
11. Never use any other except Esco Medical filter. Otherwise, the warranty will be
void.
12. Do not use the device without a proper Esco Medical VOC/HEPA filter attached.
8 Getting started
Mini MIRI®Dry or Mini MIRI® Humidity incubators must be installed by au-
thorized and trained personnel only!
1. Follow the guidelines in the safety instructions and warnings section.
2. Connect the mains cable to the UPS.
3. Connect the mains cable to Mini MIRI®Dry or Mini MIRI®Humidity incubator.
4. Connect gas sample lines.
5. Set the gas pressure on the external gas regulator at 0.4 –0.6 bar (5.80 –8.70 PSI).
6. Switch on Mini MIRI®Dry or Mini MIRI Humidity® incubator in the back.
7. Observe for standard functionality.
8. Let the unit warm up and stabilize for 20 min.
9. Follow the guidelines in the Validation guide.

Mini MIRI® Dry/Humidity incubators User Manual Rev. 1.0
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10. Complete user training and finish reading instructions.
11. After a burn-in phase of 24-hours, the unit is ready for use IF the testing is suc-
cessful.
If the device is going to be used in a clinical setting, clean and disinfect the de-
vice before use. It is not delivered sterile or in a clinically acceptable clean state.
Consult the cleaning instructions section in this manual for the manufacturer’s rec-
ommended guidelines!
9 Mains connection
Mini MIRI®Dry and Mini MIRI®Humidity incubators come with a detachable main power
cord. The power cord is prepared for the country in which the unit is intended to be used.
Do not defeat the safety purpose of the grounding-type plug! A grounding type
plug has two blades and a third prong. It is provided for your safety. If the provided
plug does not fit into your outlet, consult an electrician to replace the outlet.
The power requirement is 230V 50 Hz OR 115V 60Hz. The built-in power supply has a
switch-mode that automatically adjusts to the correct mains power between 100V-250V
AC 50-60 Hz.
Figure 9.1 Power supply
10 Gas connections
There are two gas inlets on the back of the unit. These ports are marked “N2100% Inlet”
and “CO2100% Inlet”.
Figure 10.1 Gas inlets

Mini MIRI® Dry/Humidity incubators User Manual Rev. 1.0
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CO2 inlet should be connected to a 100% concentration CO2. CO2 control in the
compartment is available in the range from 1.9% to 9.9%.
The N2inlet should be connected to 100% concentration N2if low oxygen conditions are
required. The O2control in the compartments is available in the range from 3.9% - 19.9%
by infusing N2.
The premixed gas inlet should be connected to the CO2inlet.
Gas pressure for both inlets should be between 0.4 –0.6 bar (5.80 –8.70 PSI)
and it must be kept stable!
Always use a high-quality pressure regulator that can be set with the required precision
for both gases.
Figure 10.2 Pressure regulator
Connect the CO2gas to the CO2inlet with a suitable silicone tube. Ensure the tube is fas-
tened with a clip so that it does not accidentally loosen itself during sudden pressure fluc-
tuation. Use the supplied 0.2µ HEPA filter on the gas line just before the inlet on the incu-
bator. Notice the direction.
Connect the N2inlet to the Nitrogen Bottle / CO2inlet to the premixed Bottle.
Figure 10.3 Gas filter
Mini MIRI®Dry or Mini MIRI®Humidity incubators can also run-on premixed
gas. It is a more expensive option for gas consumption. It also means the user can-
not adjust the CO2and O2levels without changing the gas supply. Please read in the
“13 Installation with premixed gas” section below for more information about us-
ing the device on premixed gas.

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11 HEPA/VOC filter (not available in the Mini MIRI®Humidity)
VOCs are hydrocarbon-based compounds that are found in fuel, solvents, adhesives, and
other compounds. Examples of VOCs include isopropanol, benzene, hexane, formalde-
hyde, vinyl chloride.
VOCs can also occur in medical gases, such as CO2and N2. It is essential to use in-line VOC
filters for your medical gases to prevent these fumes from entering your incubators.
Unexpected sources of VOCs are commonly found in IVF labs. These can include cleaning
agents, perfumes, cabinetry, grease on the wheels of equipment, and sources in HVAC
equipment.
VOCs are typically measured in parts per million (ppm). They can also be reported in
parts per billion (ppb). For IVF labs, VOCs' recommended count is below 0.5 ppm; the
total quantity of VOCs should be <0.2 ppm or preferably zero.
High levels of VOCs (over 1 ppm) are toxic to embryos, resulting in poor embryo devel-
opment and even probable failure to reach the blastocyst stage.
VOC levels in the 0.5 ppm range will typically allow for acceptable blastocyst develop-
ment and reasonable pregnancy rates but will result in a high percentage of miscarriages.
A combined HEPA and VOC filter (carbon filter) are integrated into the Mini MIRI® Dry
incubator's construction. Before entering the incubator, the gas is sent through the filter
in a single pass. Then upon return from the compartment, the gas is filtered again. The
recirculation system constantly filters the gas present in the incubator.
The combined HEPA and VOC filter is mounted on the back of the device for easy access
and replacement (only in Mini MIRI®Dry incubator).
11.1 Installation of new filter capsule (only in the Mini MIRI®Dry incubator)
The two blue caps that are installed on the filter can be discarded during unwrapping.
Correct filter performance is crucial for system performance.
Filter element must be changed every 3 months. Mark the date when it is put
on and make sure to keep this interval!
Start by putting the blue fittings on the filter into the filter holder sockets. The flow arrow
on the incubator and the filter should point in the same direction.

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Figure 11.1 The flow arrow on the incubator
Figure 11.2 The way of pulling the filter
Figure 11.3 Filter in place
Then, simultaneously press both angle fittings (using both hands) into the holes till they
snap into place. The last 4 mm step should feel stiff.
A filter element that is fitted incorrectly will cause the unit not to work not as
intended. This is dangerous!
The filter is removed by gently pulling it straight out using both hands.
Never run the Mini MIRI®Dry incubator with the filter element missing! Dan-
gerous particle contamination could occur!
12 User interface
In the following chapters, the functions associated with the keys and menu items will be
explained.
User interface handles daily used functions and more advanced adjustments that might
be made to the device. The main keys and their purpose are presented in table 12.1.

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Table 12.1 The main keys and their purpose
Description
Image
Rotary button
Use to toggle and select items on the menu to
change their status. It is also used to change the
temperature and gas set points values
ON/OFF button
It is located in the REAR of the unit
Alarm key
It mutes an audible alarm and visually indicates the
alarm condition by a flashing red circle of light. The
audio alarm will come back on automatically after
5 min. It can be muted again
Display panel
Shows the information on the current status of the
unit. The display consists of 7 x high brightness 16
segment LEDs. The first one is red to indicate a user
warning. The other 6 are blue and used to display
normal running conditions
12.1 Activating the heat and gas controls
Heat and gas control systems are activated using the “ON/OFF” switch in the rear.
Soon after system activation the main display will alternate the reading between the
following 4 parameters:
Temperature = Temperature in °C
CO2= CO2Concentration in %
O2 = O2Concentration in %
Mode = Open/ Oil Culture
12.2 System menu
Press and hold rotatory button for 3 seconds to access menu.
Navigate in the menu by:
•Rotating rotatory button clockwise (↻) or anticlockwise (↺) = previous OR next
•Pressing rotatory button = enter, change OR accept
Rotate the rotatory button (↺) to exit the menu entirely.