Esco Medical MIRI TL 6 User manual

Rev. 1.7
Rx only
Multi room incubator MIRI®TL 6
CE 1023

MIRI®TL 6 User Manual Rev. 1.7
2
For Technical Service, contact
North America
Esco Technologies, Inc.
2940 Turnpike Drive, Units 15-16 •Hatboro, PA 19040, USA
Toll-Free USA and Canada 1-877-479-3726
Tel [phone] •Fax [phone]
us.escoglobal.com •[email protected]
Rest of World
Esco Micro Pte. Ltd.
21 Changi South Street 1 •Singapore 486 777
Tel [phone] •Fax [phone]
www.escoglobal.com •[email protected]
Copyright Information
© Copyright 2014 Esco Micro Pte Ltd. All rights reserved.
The information contained in this manual and the accompanying product is copyrighted and
all rights are reserved by Esco.
Esco reserves the right to make periodic minor design changes without obligation to notify
any person or entity of such change.
Fertilisafe®and Sentinel™ are registered trademarks of Esco.
Caution: Federal law restricts this device to sale by or on the order of a licensed healthcare
practitioner.
Only to be used by a trained and qualified professional. The device is sold under exemption
21 CFR 801 Subpart D.
“Material in this manual is provided for informational purposes only. The contents and the
product described in this manual (including any appendix, addendum, attachment or
inclusion), are subject to change without notice. Esco makes no representations or warranties
as to the accuracy of the information contained in this manual. In no event shall Esco be held
liable for any damages, direct or consequential, arising out of or related to the use of this
manual.”

MIRI®TL 6 User Manual Rev. 1.7
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Unpacking and Inspection
Follow standard receiving practices upon receipt of the instrument. Check the shipping
carton for damage. If damage is found, stop unpacking the instrument. Notify the freight
carrier and ask for an agent to be present while the instrument is unpacked. There are no
special unpacking instructions, but be careful not to damage the instrument when un-
packing it. Inspect the instrument for physical damage such as bent or broken parts,
dents, or scratches.
Claims
Our routine method of shipment is via common carrier. Upon delivery, if physical damage
is found, retain all packing materials in their original condition and contact the carrier
immediately to file a claim.
If the instrument is delivered in good physical condition but does not operate within spec-
ifications, or if there are any other problems not caused by shipping damage, please con-
tact your local sales representative or Esco Medical immediately.
Standard Terms and Conditions
Refunds & Credits
Please note only serialized products (products labelled with a distinct serial number) and
accessories are eligible for partial refund and/or credit. Non-serialized parts and acces-
sory items (cables, carrying cases, auxiliary modules, etc.) are not eligible for return or
refund. In order to receive a partial refund/credit, the product must not have been dam-
aged, and must be returned complete (meaning all manuals, cables, accessories, etc.)
within 30 days of original purchase, in “as new” and resalable condition. The Return Pro-
cedure must be followed.
Return Procedure
Every product returned for refund/credit must be accompanied by a Return Material Au-
thorization (RMA) number, obtained from Esco Medical Customer Service. All items being
returned must be sent prepaid (freight, duty, brokerage and taxes) to our factory location.
Restocking Charges
Products returned within 30 days of original purchase are subject to a minimum restock-
ing fee of 20% of the list price. Additional charges for damage and/or missing parts and
accessories will be applied to all returns. Products which are not in “as new” and resalable
condition, are not eligible for credit return and will be returned to the customer at their
own expense.
Certification
This instrument has been thoroughly tested/inspected and found to meet Esco Medical´s
manufacturing specifications when shipped from the factory. Calibration measurements
and testing are traceable and done according to Esco Medicals ISO certification.
Warranty and Product Support
Esco Medical warrants this instrument to be free from defects in materials and workman-
ship under normal use, and service for two (2) years from the date of original purchase,
provided the instrument is calibrated and maintained in accordance with this manual.
During the warranty period Esco Medical will, at our option, either repair or replace a

MIRI®TL 6 User Manual Rev. 1.7
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product that proves to be defective at no charge, provided you return the product (ship-
ping, duty, brokerage and taxes prepaid) to Esco Medical. Any and all transportation
charges incurred are the responsibility of the purchaser and are not included within this
warranty. This warranty extends only to the original purchaser and does not cover dam-
age from abuse, neglect, accident or misuse, or as the result of service or modification by
parties other than Esco Medical.
IN NO EVENT SHALL ESCO MEDICAL LTD. BE LIABLE FOR CONSEQUENTIAL DAMAGES.
No warranty shall apply when damage is caused by any of the following:
•Power failure, surges, or spikes
•Damage in transit or when moving the instrument
•Improper power supply such as low voltage, incorrect voltage, defective wiring or in-
adequate fuses
•Accident, alteration, abuse or misuse of the instrument
•Fire, water damage, theft, war, riot, hostility, acts of God such as hurricanes, floods, etc.
Only serialized products (those items bearing a distinct serial number tag) and their ac-
cessory items are covered under this warranty.
PHYSICAL DAMAGE CAUSED BY MISUSE OR PHYSICAL ABUSE IS NOT COVERED UNDER
THE WARRANTY. Items such as cables and non-serialized modules are not covered under
this warranty.
This warranty gives you specific legal rights and you may have other rights, which vary
from province to province, state to state, or country to country. This warranty is limited
to repairing the instrument per Esco Medical's specifications.
When you return an instrument to Esco Medical for service, repair or calibration, we rec-
ommend shipment using the original shipping foam and container. If the original packing
materials are not available, we recommend the following guide for repackaging:
•Use a double-walled carton of sufficient strength for the weight being shipped
•Use heavy paper or cardboard to protect all instrument surfaces. Use non-abrasive ma-
terial around all projecting parts
•Use at least four inches of tightly packed, industrial-approved, shock-absorbent mate-
rial all around the instrument
Esco Medical will not be responsible for lost shipments or instruments received in dam-
aged condition due to improper packaging or handling. All warranty claim shipments
must be made on a prepaid basis (freight, duty, brokerage, and taxes). No returns will be
accepted without a Return Materials Authorization ("RMA”) number. Please contact Esco
Medical to obtain an RMA number and receive help with shipping/customs documenta-
tion.
Re-calibration of instruments, which have a recommended annual calibration frequency,
is not covered under the warranty.

MIRI®TL 6 User Manual Rev. 1.7
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Warranty Disclaimer
Should you choose to have your instrument serviced and/or calibrated by someone other
than Esco Medical Ltd. and their representatives, please be advised that the original war-
ranty covering your product becomes void when the tamper-resistant Quality Seal is re-
moved or broken without proper factory authorization.
In all cases, breaking the tamper-resistant Quality Seal should be avoided at all cost, as
this seal is key to your original instrument warranty. In an event where the seal must be
broken to gain internal access to the instrument, you must first contact Esco Medical Ltd.
You will be required to provide us with the serial number for your instrument, as well as
a valid reason for breaking the Quality Seal. You should break this seal only after you have
received factory authorization. Do not break the Quality Seal before you have contacted
us! Following these steps will help ensure that you will retain the original warranty on
your instrument without interruption.
WARNING
Unauthorized user modifications or application beyond the published specifications may
result in electrical shock hazard or improper operation. Esco Medical will not be respon-
sible for any injury sustained due to unauthorized equipment modifications.
ESCO MEDICAL LTD. DISCLAIMS ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED,
INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR
PURPOSE OR APPLICATION.
THIS PRODUCT CONTAINS NO USER-SERVICEABLE COMPONENTS.
UNAUTHORIZED REMOVAL OF THE INSTRUMENT COVER SHALL VOID THIS AND ALL
OTHER EXPRESSED OR IMPLIED WARRANTIES.

MIRI®TL 6 User Manual Rev. 1.7
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Table of contents
1 How to use this manual............................................................................................................................ 9
2 Safety Warning............................................................................................................................................ 9
3 Indication for use........................................................................................................................................ 9
4 About the product ....................................................................................................................................10
5 Accessories supplied...............................................................................................................................11
6 Manuals supplied......................................................................................................................................11
6.1 The user manual...............................................................................................................................11
6.2 The validation manual ...................................................................................................................12
6.3 The maintenance manual..............................................................................................................12
7 Safety symbols and labels .....................................................................................................................12
8 Important Safety Instructions and warnings ................................................................................14
8.1 Before installation ...........................................................................................................................14
8.2 During installation...........................................................................................................................14
8.3 Post installation................................................................................................................................15
9 Getting started...........................................................................................................................................15
10 Mains connections.................................................................................................................................16
11 Gas connections......................................................................................................................................16
12 HEPA / VOC filter...................................................................................................................................17
12.1 Installation of a new filter capsule..........................................................................................18
12.2 Removing the old filter................................................................................................................19
13 User Interface..........................................................................................................................................19
13.1 Activating the heat and gas control........................................................................................20
13.2 Menu system ...................................................................................................................................21
13.2.1 Calibration ...............................................................................................................................22
13.2.1.1 Temperature calibration ................................................................................................23
13.2.1.2 CO2/O2calibration ............................................................................................................24
13.2.2 CO2Setup..................................................................................................................................24
13.2.3 O2Setup ....................................................................................................................................25
13.2.4 Temperature setup...............................................................................................................25
13.2.5 UVC Light..................................................................................................................................26
14 Alarms........................................................................................................................................................27

MIRI®TL 6 User Manual Rev. 1.7
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14.1 Temperature alarms ....................................................................................................................27
14.2 Gas level alarms .............................................................................................................................29
14.3 Gas pressure alarms.....................................................................................................................30
14.4 UVC light alarm ..............................................................................................................................31
14.5 Multiple alarms ..............................................................................................................................31
14.6 Loss of power alarm.....................................................................................................................32
15 Changing the set points .......................................................................................................................32
15.1 Temperature set point ................................................................................................................32
15.2 CO2Setpoint.....................................................................................................................................36
15.3 O2Setpoint .......................................................................................................................................40
16 Surface temperatures and calibration...........................................................................................43
16.1 Temperature calibration example..........................................................................................44
17 Gas pressure and calibration.............................................................................................................46
17.1 Gas calibration example..............................................................................................................47
18 Firmware...................................................................................................................................................48
19 pH measuring (function not available in the US)......................................................................48
20. Screen functions....................................................................................................................................50
20.1 Start a time-lapse...........................................................................................................................53
20.2 The calibration processes ..........................................................................................................56
20.3 The compartment view...............................................................................................................58
20.4 Settings..............................................................................................................................................61
20.5 Adjustments live View.................................................................................................................62
20.6 Warnings...........................................................................................................................................67
20.7 The data-logging temperature view.......................................................................................71
20.8 The data-logging CO2view.........................................................................................................72
20.9 The data-logging O2view ...........................................................................................................72
20.10 The data-logging alarm view..................................................................................................73
21 CultureCoin® ..........................................................................................................................................74
22 MIRI®TL 6 Viewer ................................................................................................................................76
23 Cleaning instructions ...........................................................................................................................76
23.1 Considerations about a sterile device ...................................................................................76
23.2 The manufacturer recommended cleaning procedure...................................................76
23.3 The manufacturer recommended disinfection procedure............................................77

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24 Temperature validation ......................................................................................................................78
25 Gas level validation ...............................................................................................................................78
26 Alarm switches for an external system.........................................................................................79
27 Writing area on the compartment lids..........................................................................................80
28 Maintenance ............................................................................................................................................81
29 Emergency Procedures........................................................................................................................81
30 User Troubleshooting ..........................................................................................................................83
31 Specifications...........................................................................................................................................85
32 Electromagnetic Compatibility.........................................................................................................86

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1 How to use this manual
The manual is designed to be read by sections, and not ideally cover to cover. This means
that if the manual is read, from start to finish, there will be some repetition and overlap. We
recommend the following method to go through the manual: first, familiarize yourself with
the safety instructions; then proceed to the basic user functions that are needed for operat-
ing the equipment on a day to day basis; then review the alarm functions. The menu function
of the user interface details information that is needed for the advanced level of users only.
All parts must be read before the device is taken into use.
2 Safety Warning
•Anyone working with, on or around this equipment should read this manual. Failure to
read, understand and follow the instructions given in this documentation may result in dam-
age to the unit, injury to operating personnel, and/or poor equipment performance.
•Any internal adjustment, modification or maintenance to this equipment must be under-
taken by qualified service personnel.
•If the equipment must be relocated, make sure it is fixed properly on a support stand or
base, and move on a flat surface. When necessary move the equipment and the support
stand/base separately.
•The use of any hazardous materials in this equipment must be monitored by an industrial
hygienist, safety officer or other suitably qualified individual.
•Before you proceed, you should thoroughly understand the installation procedures and
take note of the environmental/electrical requirements.
•In this manual, important safety related points will be marked with the following symbols:
NOTE
Used to direct attention to a specific item.
WARNING
Use caution
•If the equipment is used in a manner not specified by this manual, the protection provided
by this equipment may be impaired.
3 Indication for use
To provide an environment with controlled temperature, CO2(and other gases) for the de-
velopment of embryos. This model has an integrated inverted microscope and imaging sys-
tem for embryo viewing. Device use is limited up to five days (120 hour), covering the time
from post-fertilization to day-5 of development.

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4 About the product
The Esco Medical MIRI® TL 6 is a multi-room CO2/O2 incubator with time-lapse capability,
making it possible to incubate up to 84 embryos and generate time-lapse microscopy images
of the development stages during the incubation.
Direct warming of the dishes in the chambers provides superior temperature conditions in
comparison to conventional incubators.
The temperature will stay stable to 1oC in the compartment even when a lid is left open for
30-seconds, and recovers within 1 minute after closing the lid.
The MIRI® TL 6 incubator has 6 completely independent culture heated chambers, each
chamber with its own heated lid, and room for 1 CultureCoin® dish.
For maximum performance the system has 12 completely independent PID temperature
controllers. PID controls and regulates the temperature in the culture chambers and the lids.
Temperatures in individual compartments do not affect the temperatures in other
compartments. Top and bottom of each compartment is separated with a PET layer, so that
the lid temperature does not affect the bottom. For validation purposes each compartment
has a built-in PT-1000 sensor. The circuitry is separated from the unit’s other electronics, so
it remains a truly independent validation system.
The Incubator needs 100% CO2 and 100% N2 to be able to control the CO2 and O2
concentrations in the culture chambers.
A dual beam infra-red CO2 sensor with extremely low drift rate controls the CO2 level. A
chemical medical-grade oxygen sensor controls the O2 regulation.
The gas recovery time is less than 3 minutes after the opening of a lid. For gas concentration
validation, the incubator is fitted with 6 gas sample ports that allow the user to sample gas
from individual compartments.
The incubator features a re-circulated gas system where the gas flows in and out of the
compartment at the same rate. The gas is cleaned via a 254 nm UVC light with direct gas
contact between the bulb and the gas, then through a VOC filter and a HEPA filter. The UVC
light has filters to inhibit 185 nm radiation that would produce dangerous ozone. The VOC
filter is located after the UVC light.
Complete gas repletion in the system takes less than 5 min.
The total gas consumption is very low: CO2<2 l/h and N2<5 l/h.
For safety the incubator has a complete gas control system: A pressure regulator (preventing
dangerous gas pressure problems), gas flow sensors (actual consumption can be
accumulated), gas pressure sensors (so that the user knows the pressure, and variation can
be logged), gas filters (to avoid valve problems).
It is easy and safe to place a CultureCoin® dish in the compartment, the compartments are

MIRI®TL 6 User Manual Rev. 1.7
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numbered and you can write on the white lid with a pen.
The incubator has been primarily designed for incubation of embryos with an overlay of
either Paraffin or mineral oil.
A large and clear LED display that is easy to read from a distance. The user can see the
parameters without having to go nearer to the unit.
The software uses the built-in touch screen. A PC controls the microscopy system which can
generate one image per every 5-minutes. These images can be compiled and viewed as a
time-lapse movie.
The Software contains logging functions for long term data logging and data storage. A web
module makes the QC data transferable for off-site evaluation (the QC evaluation can be by
the manufacturer as a service to the customer). A pH sensor port is part of the DAQ package.
The user can plug any standard BNC pH probe to the unit and measure the sample pH.
The device is manufactured under a fully EU certified 13485 ISO quality management
system.
This product meets the requirements of EN60601-1 3rd edition standards as a Class I type B
equivalent device suited for continuous operation. It also conforms to the requirements of
the EU Council directive 93/42/EEC concerning medical devices and is classified as a Class
IIa device under rule II.
5 Accessories supplied
•1 VOC/HEPA filter capsule
•2 HEPA filters for input gas supply
•1 USB stick containing Esco Medical Data logger software and PDF versions of the manuals
•1 data cable
6 Manuals supplied
The MIRI®TL 6 incubator comes with 3 manuals as standard:
1. The User Manual (this manual)
2. The Validation Manual
3. The Maintenance Manual
Each manual has a specific purpose.
6.1 The user manual
Is intended to provide the user with information necessary for using the device.
The user manual cannot stand alone nor replace user training.

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6.2 The validation manual
Is intended for trained technical personnel performing the installation, detailing the proce-
dures and specifications needed to ensure that the device is safe and can be taken into clin-
ical use. The manual can also be used as a guide for validation tests that should be performed
regularly.
6.3 The maintenance manual
Is also intended for trained technical personnel, detailing the schedules and methods to en-
sure that the device runs optimally and safely during its lifespan.
The maintenance specified in the maintenance manual cannot replace the regu-
lar maintenance/validation that must be performed by the user.
7 Safety symbols and labels
There are several user labels on the surfaces of the MIRI®TL6 to guide the user. User labels
are shown below.
Table 7.1 Labels
Description
Image
Packing box label:
- If stored longer than one year from manufac-
ture date, the unit must be returned to the man-
ufacturer for a new release test
- Store between -20oC and +55oC
- Keep away from direct sunlight
- Caution: consult the accompanying docu-
ments for important safety-related information
such as warnings and precautions that cannot,
for a variety of reasons, be presented on the de-
vice itself
- Consult instructions for proper use of the de-
vice
- Do not use if the packing material is damaged
- Rx Only
- Keep dry

MIRI®TL 6 User Manual Rev. 1.7
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- Warning on the back of the incubator indicates
that an earth connection is needed, mains infor-
mation, and “ON/OFF” push-push button
- ‘Lightning bolt’indicates the potential risk of
electrical shock (never remove any cover)
- Model
- Mains power rating
- CE mark
- Not protected against ingress of water
- Manufacturers address and country of origin
- Logo and serial number
- Lot number
- Caution when discarded
- Observe WEEE
- Year of manufacture
Table 7.2 Labels on the back of MIRI®TL 6
Description
Image
USB communication port
CO2inlet
N2inlet
BNC pH
Alarm port
Compartments numbers are indicated in the
top corner of the lid with a label
Maximum pressure 0.8 bar
VOC/Hepa filter
Ethernet
TL on/off
Compartment numbers are shown in the picture below and also indicated on the top of the
lid with labels:

MIRI®TL 6 User Manual Rev. 1.7
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Figure 7.3 Compartments numbers layout
8 Important Safety Instructions and warnings
8.1 Before installation
1. Do not use the product if the package is damaged. Contact Esco Medical or the local rep-
resentative
2. Read the user manual completely before use
3. Always keep these instructions easily accessible near the device
8.2 During installation
1. Never place this unit on top of other equipment that might heat it up
2. Place this unit on a flat, hard and stable surface
3. Never place the unit on a carpet or similar surfaces
4. Do not defeat the safety purpose of the grounding-type (earthing) plug
5. A grounding-type (earthing) plug has two blades and a third prong is provided for your
safety. If the provided plug does not fit into your outlet, consult an electrician to replace the
outlet
6. Always connect the power cord to a properly grounded outlet and only use the cord that
came with the device
7. Do not install near any heat sources such as radiators, heat registers, stoves or other ap-
paratus that produce heat
8. Do not use this device near water
9. Use only 100% concentration CO2and 100% concentration N2gas
10. Always use an external HEPA filter for input CO2and N2gas
11. Do not use this product at temperatures exceeding 30oC
12. Place this unit in a location with adequate ventilation to prevent internal heat build-up.
Allow at least 10 cm clearance from the rear, 30 cm from the top and 20 cm from left and
right to prevent overheating, and to allow access to the on/off switch in the back
13. This unit is intended for indoor purposes only
14. The unit must be connected to a suitable Uninterrupted Power Supply (UPS)

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8.3 Post installation
1. Refer all the servicing to qualified service personnel
2. Servicing is required according to the service manual, or if the apparatus has been dam-
aged in any way, e.g. if the apparatus has been dropped, exposed to rain or moisture, or does
not operate normally. The MIRI®TL 6 incubator contains high voltage components that may
be hazardous
3. Unplug this apparatus during lightning storms or when unused for a long period of time
4. Protect the power cord from being walked on or pinched, particularly at the plug, conven-
ience receptacles, and the point where it exits from the apparatus
5. Perform temperature and gas calibration at the intervals described in the manuals
6. UV lamp must be changed at the interval described in the manuals (not available in the
US)
7. O2sensor must be changed at the interval described in the manuals
9. Never leave lids open for more than 10 seconds while in use
10. VOC/HEPA filters must be changed at the interval described in the manuals
11. NEVER block gas supply holes in the compartment
12. Make sure that CO2and N2gas supply pressures are kept stable at 0.6 bar (8.70 PSI)
13. Never use a non-Esco Medical filter; it will void the warranty
14. Do not use the product without a proper Esco Medical VOC/HEPA filter attached
9 Getting started
The MIRI®TL 6 incubator must be installed by authorized and trained personnel
only.
1. Follow the guidelines in the safety instructions and warnings section
2. Connect the mains cable to the UPS
3. Connect the mains cable to the MIRI®TL 6 incubator
4. Connect the gas lines
5. Set the gas pressure on the external gas regulator at 0.6 bar (8.70 PSI)
6. Switch on the MIRI®TL 6 in the back
7. Observe for normal function
8. Let the unit warm up and stabilize for 20 min
9. Follow the guidelines in the validation guide
10. Complete user training and instructions
11. After a burn-in phase of 24-hours, the unit is ready for use IF the testing is successful
Clean the device before use. It is not delivered sterile or in clinically acceptable
cleanliness state. Consult the cleaning instructions section in this manual for the
manufacturers recommended guidelines.

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10 Mains connections
The MIRI® TL 6 incubator comes with a detachable mains power cord. The power cord is
prepared for the country in which the unit is intended to be used in.
Do not defeat the safety purpose of the grounding-type plug! A grounding type
plug has two blades, and a third prong. It is provided for your safety. If the provided
plug does not fit into your outlet, consult an electrician to replace the outlet.
The power requirement is 230V 50 Hz OR 120V 60Hz. The built-in power-supply is of a
switch mode type, that automatically adjusts to the correct mains power between 100V-
250V AC 50-60 Hz.
Figure 10.1 Power supply
11 Gas connections
On the back of the unit there are two gas inlets. These ports are marked “CO2” and “N2”.
Figure 11.1 CO2 and N2 gas inlets
The CO2 inlet should be connected to 100% concentration CO2. The N2 inlet should be
connected to 100% concentration N2 if low oxygen conditions are required. Oxygen levels in
the compartments can be maintained in the range of 4.9% - 20% by infusing N2.
Gas pressure for both inlets should be between 0.4-0.7 bar (5.8-10.2 PSI) and it
must be kept stable.

MIRI®TL 6 User Manual Rev. 1.7
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For both gases, always use a high-quality pressure regulator that can be set with the
necessary precision.
Figure 11.2 Pressure regulator
Connect the CO2 gas to the CO2 inlet with a suitable silicone tube. Make sure that the tube is
secured with a clip, so that it does not accidentally loosen during sudden pressure
fluctuation. Use the supplied 0.2µ HEPA filter on the gas line just before the inlet on the
incubator.
Figure 11.3 Gas filter
Connect the N2 inlet to the Nitrogen Bottle in the same way as for the CO2 inlet.
12 HEPA / VOC filter
VOCs are hydrocarbon-based compounds that are found in fuel, solvents, adhesives, and
other compounds. Examples of VOCs are isopropanol, benzene, hexane, formaldehyde, vinyl
chloride.
VOCs can also occur in medical gases, such as CO2and nitrogen. It is important to use inline
VOC filters for your medical gasses to prevent these fumes from entering your incubators.
Unexpected sources of VOCs are commonly found in IVF labs. These can include cleaning
agents, perfumes, cabinetry, grease on the wheels of equipment, and sources in HVAC equip-
ment.
VOCs are typically measured in parts per million (ppm.) They can also be reported in parts
per billion (ppb.) For IVF, count below 0.5 ppm is recommended; total VOCs should be below
<0.2 ppm, or preferably zero.
High VOCs levels (over 1 ppm) are toxic to embryos: You will see poor mouse test results
and very poor human embryo development, probably not even making it to blastocyst stage.

MIRI®TL 6 User Manual Rev. 1.7
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VOC levels in the 0.5 ppm range will typically allow for acceptable blastocyst development
and reasonable pregnancy rates but will result in a high percentage of miscarriages.
A combined HEPA and VOC filter (carbon filter) is integrated in the construction of the MIRI®
TL6. Prior to entering the incubator, the gas is sent through the filter in a single pass. Then
upon return from the compartment the gas is filtered again. The recirculation system con-
stantly filters the gas present in the incubator.
The combined HEPA and VOC filter is mounted on the back of the device for ease of access
and replacement.
12.1 Installation of a new filter capsule
The two blue caps that are installed on the filter can be discarded during unwrapping.
Correct filter performance is crucial for the system performance.

Filter element must be changed every 3 months. Mark the date when it is put on
and make sure to keep this interval!
Start by putting the blue fittings on the filter into the filter holder sockets. The flow arrow
on the incubator and the filter should point in the same direction.
Figure 12.1.1 Device view without VOC/HEPA filter
Then simultaneously press-in both angle fittings (using both hands) into the holes till they
snap into place. The last 4 mm step should feel stiff.
Figure 12.1.2 Correctly assembled VOC/HEPA filter

MIRI®TL 6 User Manual Rev. 1.7
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A filter element that has been fitted incorrectly will cause the unit not to work as
intended. This is dangerous.
12.2 Removing the old filter
The filter is removed by gently pulling it straight out using both hands together.
Never run the MIRI® TL 6 with the filter element missing! Dangerous particle
contamination could occur!
13 User Interface
In the next chapters, the functions associated with the keys and the menu items are
explained. The user interface handles the daily use functions and the more advanced
adjustments that are possible on the device.
Description
Image
On/off
At the REAR of the unit
Alarm key
Is used to mute an audible alarm and indicates the
alarm condition visually by a flashing red circle of
light. The audio alarm will automatically come
back on after 5 min; it can be muted again
Touch display panel
Shows the information, about the current status
of the unit. The display is used to navigate
through the menu, and change values for
temperature and gas concentrations

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13.1 Activating the heat and gas control
The heat and gas control are activated using the power On/Off switch at the rear.
Figure 13.1.1 MIRI®TL 6 rear
‘Please wait’message appears on the screen while system is booting up for work.
Figure 13.1.2 Loading view
Soon after system activation the main display will show the following 3 parameters
Temperature = Temperature in oC
CO2 = CO2concentration in %
O2 = O2concentration in %