Fluigent LineUp Series User manual

USER’S
MANUAL
LINEUPTM SERIES

Push-Pul lPush-Pull
QUICK START GUIDE
LineUp Flow EZTM
Pressure controller
LineUpTM Push-Pull
Pressure controller
Aria is a perfusion system which automates perfusion or timed
injection protocols. It allows for the sequential delivery of up to 10
different solutions at the desired flow rate into a microfluidic chip,
perfusion chamber or petri dish.
The quick start guide is a forward to the LineUpTM Series user's manual

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A   -  
QUICK START GUIDE
1Supplied (inlet) pressure from pump or compressed air supply
2Power supply to the Flow EZTM / Push-Pull pressure controller
3Regulated (outlet) pressure from pressure controller to reservoir
4Pressurized reservoir P-CAP
5Microfluidic set-up in example a microfluidic chip
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2
4
3
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1. The Flow EZTM/ Push-Pull regulates the externally supplied
(inlet) pressuretoauser-definedpressurewithhighprecision.
2. Thisregulated(outlet)pressurepressurizesthereservoir,
driving the liquid up through the tubing and into the
microfluidicsetup.
3. Controllingtheoutletpressureallowsonetocontroltherate
atwhichfluidisinjectedintothemicrofluidicsetup.

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W  
QUICK START GUIDE
Contained in the package
Flow EZTM or Push-Pull Outlet tubing (OD 4mm)
*OD: Outer diameter
P-CAP Kit or Fluiwell Kit (sold separately)
P-CAP (or Fluiwell) reservoir
Fluiwell: for 15mL or 50mL reservoirs
P-CAP for 2mL, 15mL or 50mL reservoirs
Fluidic tubing
Outer diameter and inner diameter
depending on the Kit

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QUICK START GUIDE
LineUpTM supply Kit (sold separately)
Inlet tubing (OD: 6mm)
Power supply (24VDC)
Power supply to Sub-D adaptor
*OD: Outer diameter

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P 
QUICK START GUIDE
Oncepoweredon,theFlowEZTMor
Push-Pull willdisplaythe "Inlet
pressure check window",givingthe
measuredpressureattheinlet
(P inlet)andthesupplypressure
requiredtooperatethepressure
controller (Ptarget).
For example, left shows a 1000 mbar
range Flow EZTM
P target : 1100 mbar
P inlet : 0 mbar
Note : For the 7000 mbar range as
well as negative pressure ranges
Flow EZTM, this window will not appear.
To wall socket

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P 
QUICK START GUIDE
The Flow EZTM or Push-Pull
needs an external pressure
supply to operate. This can be
a lab's compressed air supply,
an air compressor, or any
clean(filtered< 10 µm)anddry
compressed air supply.
Different Flow EZTM ranges
require different supply
pressures(showntotheright).
Note : If one intends to use compressed gas other than air, or if the pressure
supply is out of the pressure range requirements, please contact Fluigent.
Always wear safety protection
when manipulating pressure.
- For positive range, never
exceed 8 bar (120 psi)
- For negative range, never
connectanypositivepressure
Pressure supply
Flow EZTM
range (mbar)
Required pressure
supply (mbar)
7000 7100
2000 2100
1000
1100
345
69
150
25
-25
-800
-69
-345
-800

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The Push-Pull module works the same way as other Flow EZTM modules,
except that it can output pressure (above atmospheric pressure)
and vacuum (below atmospheric pressure) through the same port.
P-P 
Note : Depending on the supply pressures, the performance of
the module can vary, especially for the vacuum pressure supply.
When connecting the two supplies, only the positive pressure supply
will be checked on the "Inlet pressure check window". (see page 10)
Push-Pull output (mbar) Required pressure supply (mbar)
1000 1100
-800 -800
QUICK START GUIDE
Positive pressure
supply inlet
Vacuum
supply inlet
Pressure /
Vacuum outlet
To do so, it needs two inlets: the positive one, uses the standard pressure
supply of the Flow EZTM, and the negative one, utilizes an additional
connector located at the front between the FLOW UNIT port and the
"unlock" handle.
Ensure to power ON the LineUpTM Push-Pull and set the pressure
value on 0 mbar before connecting the vacuum supply.
Special attention
should be taken to
preventliquidfrom
flowing back into
the module.

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QUICK START GUIDE
A Push-Pull module can be combined with other positive
pressure LineUPTM modules the same way as a Flow EZTM
1 bar. If connecting to higher pressure modules, please
consider adding an Adapt module to reduce the
positive pressure supply. (See page 29 for more details)
When using a Push Pull module, care should be taken to
avoid drawing liquid into the system. This can seriously
damage the module. It is advised to use a liquid filter
between the outlet and the reservoir as it reduces the risks.
Note: The pressure supply is mandatory for the use of the Push-Pull even
in vacuum mode only. The vacuum supply is optionnal.
Please, never stack a Push-Pull module with a negative Flow EZTM as it
would not work.

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A   
(A 1000 mbar range Flow EZTM is shown as an example)
Adjust the pressure
from your
pressure source
The P inlet value
is displayed in
red
The P inlet value
is displayed in
green
When connecting the pressure supply, if its value (P inlet) is not set at
the required value (P target), the Flow EZTM or Push-Pull will continue to
display the "Inlet pressure check window" (with the exception of the 7000
mbar range and the negative ranges which don't have this window)
If the value is displayed in red, please adjust the pressure supply.
When the two values are close enough, the P inlet value will turn green
and the display on the Flow EZTM or Push-Pull will transition to "Operation
window"
QUICK START GUIDE