GE Amersham Typhoon User manual

Amersham™Typhoon
Operating Instructions
Original instructions

Table of Contents
41 Introduction ..........................................................................................................
51.1 About this manual ................................................................................................................................
61.2 Important user information .............................................................................................................
81.3 Regulatory information ......................................................................................................................
91.3.1 EU Directives ....................................................................................................................................
101.3.2 Eurasian Customs Union ............................................................................................................
111.3.3 Regulations for USA and Canada ...........................................................................................
121.3.4 Korean regulatory information ................................................................................................
131.3.5 Other regulations and standards ............................................................................................
151.4 Abbreviations ..........................................................................................................................................
162 Safety instructions ...............................................................................................
172.1 Radiation ...................................................................................................................................................
192.2 Safety precautions ...............................................................................................................................
272.3 Labels .........................................................................................................................................................
332.4 Emergency procedures ......................................................................................................................
362.5 Recycling information .........................................................................................................................
372.6 Declaration of Hazardous Substances (DoHS) ........................................................................
393 System description ..............................................................................................
403.1 System overview ...................................................................................................................................
433.2 Illustrations ..............................................................................................................................................
473.3 Stages ........................................................................................................................................................
483.3.1 Overview of stages ........................................................................................................................
493.3.2 Fluor stage with accessories .....................................................................................................
533.3.3 Phosphor stage with accessories ...........................................................................................
553.3.4 Multi stage with accessories .....................................................................................................
573.4 Filters ..........................................................................................................................................................
584 Amersham Typhoon Control Software .............................................................
594.1 Main window ...........................................................................................................................................
664.2 Scanning modes ...................................................................................................................................
694.3 General settings ....................................................................................................................................
724.4 Scan area functions .............................................................................................................................
764.5 Image file settings ................................................................................................................................
784.6 View Image mode .................................................................................................................................
815 Installation ............................................................................................................
825.1 Site requirements ..................................................................................................................................
855.2 Delivery and transport .......................................................................................................................
865.3 Setup ...........................................................................................................................................................
885.4 Connections ............................................................................................................................................
2 Amersham Typhoon Operating Instructions 29193226 AC
Table of Contents

916 Operation ..............................................................................................................
936.1 Preparing the Amersham Typhoon scanner ............................................................................
946.1.1 Starting the system .......................................................................................................................
976.1.2 Changing the filters .......................................................................................................................
1016.1.3 Custom filters ...................................................................................................................................
1036.2 Preparing the sample .........................................................................................................................
1046.2.1 Preparing a sample for fluorescence imaging ..................................................................
1096.2.2 Preparing a sample for phosphor imaging ........................................................................
1166.2.3 Preparing a sample for densitometry ...................................................................................
1176.3 Performing a scan ................................................................................................................................
1226.4 Procedures after a scan ....................................................................................................................
1247 Maintenance .........................................................................................................
1278 Troubleshooting ...................................................................................................
1288.1 General errors and warnings ..........................................................................................................
1298.2 Error messages ......................................................................................................................................
1319 Reference information ........................................................................................
1329.1 Specifications .........................................................................................................................................
1359.2 Software ....................................................................................................................................................
1369.3 Ordering information ..........................................................................................................................
1389.4 Health and Safety Declaration Forms .........................................................................................
140Index .......................................................................................................................
Amersham Typhoon Operating Instructions 29193226 AC 3
Table of Contents

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regula-
tory information, and intended use of the Amersham Typhoon system.
In this chapter
This chapter contains the following sections:
See pageSection
51.1 About this manual
61.2 Important user information
81.3 Regulatory information
151.4 Abbreviations
4 Amersham Typhoon Operating Instructions 29193226 AC
1 Introduction

1.1 About this manual
Purpose of this manual
The Operating Instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Scope of this manual
The Operating Instructions cover all configurations of Amersham Typhoon scanner and
the Amersham Typhoon Control Software. The illustration below shows the
Amersham Typhoon scanner.
Descriptions of Amersham Eraser and Amersham Cabinet are not covered in this docu-
ment.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text (for example, Power switch).
Amersham Typhoon Operating Instructions 29193226 AC 5
1 Introduction
1.1 About this manual

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, operating or
maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.
Intended use of the
Amersham Typhoon scanner
The Amersham Typhoon scanner is a versatile laser scanner that can be used for many
different imaging applications, including:
•imaging of storage phosphor screens
•fluorescence imaging with multiple lasers, for example for muliplex Western blotting
and two-dimensional difference gel electrophoresis (2D DIGE)
•imaging of gels with colorimetric stains
•densitometric imaging, for example of Coomassie™ blue stained gels
Amersham Typhoon scanner is intended for research use only, and shall not be used in
any clinical procedures or for diagnostic purposes.
Prerequisites
In order to operate Amersham Typhoon scanner in the way it is intended, the following
prerequisites must be fulfilled:
•You must have read and understood the safety instructions in the user documenta-
tion.
•All operations should be performed by qualified personnel who are adequately
trained.
6 Amersham Typhoon Operating Instructions 29193226 AC
1 Introduction
1.2 Important user information

•Amersham Typhoon scanner must be installed in accordance with the site require-
ments and instructions in Chapter 5 Installation, on page 81.
Safety notices
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to pro-
ceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Note:
A tip contains useful information that can improve or optimize your procedures.
Tip:
Amersham Typhoon Operating Instructions 29193226 AC 7
1 Introduction
1.2 Important user information

1.3 Regulatory information
Introduction
This section lists the regulations and standards that apply to Amersham Typhoon scanner.
Manufacturing information
The table below summarizes the required manufacturing information.
InformationRequirement
GE Healthcare Bio-Sciences AB,Name and address of manufacturer
Björkgatan 30, SE 751 84 Uppsala, Sweden
In this section
See pageSection
91.3.1 EU Directives
101.3.2 Eurasian Customs Union
111.3.3 Regulations for USA and Canada
121.3.4 Korean regulatory information
131.3.5 Other regulations and standards
8 Amersham Typhoon Operating Instructions 29193226 AC
1 Introduction
1.3 Regulatory information

1.3.1 EU Directives
Conformity with EU Directives
This product fulfills the European Directives listed below. See the EU Declaration of
Conformity for the directives and regulations that apply for the CE marking.
If not included with the product, a copy of the EU Declaration of Conformity is available
on request.
TitleDirective
Machinery Directive (MD)2006/42/EC
Electromagnetic Compatibility (EMC) Directive2014/30/EU
Low Voltage Directive (LVD)2014/35/EU
Restriction of Hazardous Substances (RoHS) Directive2011/65/EU
CE marking
The CE marking and the corresponding EU Declaration of Conformity is valid for the in-
strument when it is:
•used according to the Operating Instructions or user manuals, and
•used in the same state as it was delivered from GE, except for alterations described
in the Operating Instructions or user manuals.
Amersham Typhoon Operating Instructions 29193226 AC 9
1 Introduction
1.3 Regulatory information
1.3.1 EU Directives

1.3.2 Eurasian Customs Union
Introduction
This section contains additional regulatory information to comply with the Eurasian
Customs Union technical regulations.
Manufacturer and importer
information
The table below summarizes the manufacturer and importer information required by
the Eurasian Customs Union.
InformationRequirement
See Manufacturing informationName and address of manufacturer
Telephone: + 46 771 400 600Telephone number of manufacturer
GE Healthcare LLCImporter and/or company for obtain-
ing information about importer GE Healthcare Life Sciences
Presnenskaya nab., 10C, 12th floor
RU-123 317 Moscow, Russian Federation
Telephone 1: + 7 495 411 9714
Fax nr: + 7 495 739 6932
Email: [email protected]
10 Amersham Typhoon Operating Instructions 29193226 AC
1 Introduction
1.3 Regulatory information
1.3.2 Eurasian Customs Union

1.3.3 Regulations for USA and Canada
NRTL certification
This symbol indicates that Amersham Typhoon has been certified by a Nationally Rec-
ognized Testing Laboratory (NRTL).
NRTL means an organization, which is recognized by the US Occupational Safety and
Health Administration (OSHA) as meeting the legal requirements of Title 29 of the Code
of Federal Regulations (29 CFR), Part 1910.7.
FCC compliance
This device complies with part 15 of the FCC Rules. Operation is subject to the following
two conditions: (1) This device may not cause harmful interference, and (2) this device
must accept any interference received, including interference that may cause undesired
operation.
The user is cautioned that any changes or modifications not expressly approved
by GE could void the user’s authority to operate the equipment.
Note:
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide rea-
sonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequen-
cy energy and, if not installed and used in accordance with the instruction manual, may
cause harmful interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference in which case the user will be re-
quired to correct the interference at his own expense.
Amersham Typhoon Operating Instructions 29193226 AC 11
1 Introduction
1.3 Regulatory information
1.3.3 Regulations for USA and Canada

1.3.4 Korean regulatory information
NOTICE
Class A equipment (equipment for business use).
This equipment has been evaluated for its suitability for use in a
business environment.
When used in a residential environment, there is a concern of radio
interference.
주의사항
A급 기기 (업무용 방송통신 기자재)
이 기기는 업무용환경에서 사용할 목적으로 적합성평가를 받은
기기
로서 가정용 환경에서 사용하는 경우 전파간섭의 우려가 있습니
다.
12 Amersham Typhoon Operating Instructions 29193226 AC
1 Introduction
1.3 Regulatory information
1.3.4 Korean regulatory information

1.3.5 Other regulations and standards
Environmental conformity
This product conforms to the following environmental requirements.
TitleRequirement
Waste Electrical and Electronic Equipment (WEEE) Directive2012/19/EU
Management Methods for the Restriction of the Use of Haz-
ardous Substances in Electrical and Electronic Products.
China RoHS
Standards, machinery and
electrical equipment
Harmonized standard requirements fulfilled by this product are summarized in the table
below.
DescriptionStandard
Safety requirements for electrical equipment for mea-
surement, control, and laboratory use - Part 1: General
requirements.
EN 61010-1, IEC 61010-1,
UL 61010-1, CAN/CSA-
C22.2 No. 61010-1
Particular requirements for automatic and semi-auto-
matic laboratory equipment for analysis and other
purposes.
IEC/EN 61010-2-081,
UL61010-2-081, CAN/CSA-
C22.2 No. 61010-2-081
Electrical equipment for measurement, control and
laboratory use - EMC requirements.
EN 61326-1, IEC 61326-1,
FCC Part 15 B Class A,
ICES-003 Class A
NOTICE
This equipment is not intended for
use in residential environments
and may not provide adequate
protection to radio reception in
such environments.
Safety of machinery. General principles for design. Risk
assessment and risk reduction.
EN ISO 12100
Degrees of protection provided by enclosures.EN60529, IEC60529
Amersham Typhoon Operating Instructions 29193226 AC 13
1 Introduction
1.3 Regulatory information
1.3.5 Other regulations and standards

Regulations and standards,
products containing lasers
DescriptionRegulation / Standard
Safety of laser productsEN/IEC 60825-1
Safety of laser productsUSA 21 CFR, Chapter I,
Subchapter J, Part
1040.10 Laser Products
This instrument is a class 1 Laser Product (IEC60825-1/EN60825-1). This instrument
meets the laser radiation safety requirements specified in the Code of the Federal Reg-
ulations (21 CFR, Chapter 1, Subchapter J).
CLASS 1
LASER PRODUCT
EN60825-1 : 2014
CLASS 1
LASER PRODUCT
IEC60825-1:2014
The following lasers can be installed in the Amersham Typhoon scanner:
Maximum powerOperating powerWavelengthLaser and class
70 mW25 mW (CW)488 nmLD laser, class 3B
80 mW10 mW (CW)532 nmSHG laser, class 3B
220 mW84 mW (CW)635 nmLD laser, class 3B
290 mW50 mW (CW)685 nmLD laser, class 3B
230 mW100 mW (CW)785 nmLD laser, class 3B
The beam divergence of all laser modules is collimated.
Note:
Different lasers are installed depending on the configuration, see Main components in
Amersham Typhoon scanner, on page 132.
14 Amersham Typhoon Operating Instructions 29193226 AC
1 Introduction
1.3 Regulatory information
1.3.5 Other regulations and standards

1.4 Abbreviations
The abbreviations used in this Operating Instructions are defined as follows:
DescriptionAbbreviation
Two-dimensional Difference Gel Electrophoresis2D DIGE
Optical densityOD
Photo-multiplier tubePMT
Red Green BlueRGB
Relative humidityRH
RadioisotopeRI
Second harmonic generation nonlinear optical processSHG
Titer plateTP
Ultraviolet radiationUV
Near-infrared radiationNIR
Amersham Typhoon Operating Instructions 29193226 AC 15
1 Introduction
1.4 Abbreviations

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition, the chapter describes emergency and recovery procedures, and
provides recycling information.
WARNING
Before installing, operating or maintaining the product, all users
must read and understand the entire contents of this chapter
to become aware of the hazards involved.
In this chapter
See pageSection
172.1 Radiation
192.2 Safety precautions
272.3 Labels
332.4 Emergency procedures
362.5 Recycling information
372.6 Declaration of Hazardous Substances (DoHS)
16 Amersham Typhoon Operating Instructions 29193226 AC
2 Safety instructions

2.1 Radiation
Radiation hazard prevention
This instrument is not equipped with any radioisotope or radiation generating unit, and
is therefore not regulated by radiation hazard prevention laws. However, the instrument
is capable of scanning storage phosphor screens which may be polluted by radioisotopes.
CAUTION
If radioisotope (RI) pollution occurs, stop use of the instrument im-
mediately and follow the instructions of your radiation administra-
tor.
Controlled area
Paragraph 1 of Article 1 of the Law Enforcement Rules for Prevention of Radiation Hazards
due to Radioisotope and so forth (Prime Minister's Office ordinance No. 56) defines the
controlled area as "a place where the dose equivalent related to external radiation ex-
ceeds the dose equivalent determined by the Director General of the Science and
Technology Agency (hereinafter referred to as the Director General), the concentration
of radioisotope in the air exceeds the concentration determined by the Director General,
or the radioisotope density on the surface polluted by radioisotope exceeds the density
determined by the Director General."
Limit of superficial pollution
Paragraph 3 of Article 4 of Notice No. 15 of the Science and Technology Agency that
determines the quantity, etc. of radiating isotope specifies that the density of radioisotope
on the surface polluted by radioisotope must be one tenth of the density defined in Article
8.
Article 8 and Table 3 of this Notice define the limits as shown below:
1Superficial density of radioisotope that radiates alpha rays: 4 Bq/cm2
2Superficial density of radioisotope that does not radiate alpha rays: 40 Bq/cm2
Amersham Typhoon Operating Instructions 29193226 AC 17
2 Safety instructions
2.1 Radiation

Installation site of instrument
This instrument is capable of scanning not only storage phosphor screens but also fluo-
rescent pigment label samples (non-RI method). Therefore, it is recommended that the
user should install it outside the controlled area and use RI-indicated samples without
contacting them with storage phosphor screens directly.
However, as described above, the storage phosphor screen surface may be polluted by
radioisotope (RI), depending on the sample condition, since the instrument sticks the
sample to the 3H-compatible storage phosphor screen surface and exposes it in an
auto-radiography experiment of the 3H label sample.
The degree of superficial pollution of the storage phosphor screen is greatly influenced
by the dryness of the sample and dose of radioisotope in an experiment and may exceed
the limits mentioned in the section above.
When the instrument reads a storage phosphor screen with a polluted non-exposure
area, it may be polluted. The degree of such superficial pollution greatly differs with
users' operation conditions. Superficial pollution may exceed the limit mentioned above.
As mentioned above, install this instrument in the RI controlled area if the user
uses RI-indicated samples that will be in direct contact with storage phosphor
screens.
Note:
Removal from the controlled
area
If it is necessary to move the instrument and its laboratory, which were installed and
have been used in the controlled area, from the controlled area, make sure that the de-
gree of the superficial pollution is below the limits mentioned in Limit of superficial pollu-
tion, on page 17 above.
18 Amersham Typhoon Operating Instructions 29193226 AC
2 Safety instructions
2.1 Radiation

2.2 Safety precautions
Introduction
Amersham Typhoon scanner is powered by mains voltage and handles materials that
may be hazardous. Before installing, operating or maintaining the system, you must be
aware of the hazards described in this manual.
Follow the instructions provided to avoid personal injuries, damage to the product,
or to other personnel and equipment in the area.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Personal protection
•Power supply
•Installing and moving the system
•System operation
•Maintenance
General precautions
WARNING
Do not use the equipment if smoke, strange noises or strange odors
can be perceived, or if the equipment becomes unusually hot. This
may result in fire or electric shock.
Stop using the equipment immediately, turn off the power switch
and unplug the equipment from the power outlet. Contact your
local GE representative to request repair.
WARNING
Do not damage the power supply cord by bending, twisting, heating
or allowing them to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local GE
representative for replacements.
Amersham Typhoon Operating Instructions 29193226 AC 19
2 Safety instructions
2.2 Safety precautions

WARNING
Do not allow liquids, flammable materials or metallic objects to get
into the Amersham Typhoon. This may result in fire or electric shock.
In case of malfunction, turn off the power switch, unplug the
equipment from the power outlet, then contact you local GE repre-
sentative.
WARNING
Never detach the inner cover screwed to this instrument. If it is
detached, laser beam may leak with a risk of loss of vision.
WARNING
Never modify the interlocks in this instrument, laser beam may leak
with a risk of loss of vision.
CAUTION
If radioisotope (RI) pollution occurs, stop use of the instrument im-
mediately and follow the instructions of your radiation administra-
tor.
CAUTION
Use of controls or adjustments or performance of procedures
other than those specified herein may result in hazardous radiation
exposure.
NOTICE
Avoid any shock or vibration to the equipment, as this may damage
the equipment.
20 Amersham Typhoon Operating Instructions 29193226 AC
2 Safety instructions
2.2 Safety precautions