Geneproof CFX Connect Application guide

GeneProof a.s.
Vídeňská 119 / CZ-619 00 Brno / [phone] / [e-mail]
VERSION: MP_04_15_01 Valid from: 04-06-15 Public 1/18
www.geneproof.com
DEVICE MANUAL
Bio-Rad

CFX Connect™ Real-Time PCR Detection System 2/18
CFX96TM Real-Time PCR Detection System
Dx Real-Time System
www.geneproof.com
3.1. STARTING THE SOFTWARE ............................................................................................................................................ 3
3.2. AMPLIFICATION PROFILE PROGRAMMING ......................................................................................................................... 4
3.3. AMPLIFICATION PROFILE SAVING .................................................................................................................................... 5
3.4. PLATE PROFILE PROGRAMMING ..................................................................................................................................... 5
3.5. PLATE PROFILE SAVING ................................................................................................................................................ 9
4.1. USING THE SAVED AMPLIFICATION PROFILE ..................................................................................................................... 10
4.2. USING THE SAVED PLATE ............................................................................................................................................ 11
4.3. PLATE EDITING......................................................................................................................................................... 13
4.4. EDITED PLATE SAVING................................................................................................................................................ 14
4.5. STARTING THE PCR TEST ............................................................................................................................................ 15
5.1. TYPICAL CURVE EXAMPLES .......................................................................................................................................... 17

Manual © GeneProof a.s. 3/18
www.geneproof.com
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CFX Connect™ Real-Time PCR Detection System 4/18
CFX96TM Real-Time PCR Detection System
Dx Real-Time System
www.geneproof.com
Step
1
2
3
4
10
Temperature
95 °C
95 °C
64 °C
72 °C
GOTO 2
Time
10 min.
10 sec.
:00 min.
20 sec.
20 sec.
39x
Fluorescence reading

Manual © GeneProof a.s. 5/18
www.geneproof.com

CFX Connect™ Real-Time PCR Detection System 6/18
CFX96TM Real-Time PCR Detection System
Dx Real-Time System
www.geneproof.com

Manual © GeneProof a.s. 7/18
www.geneproof.com

CFX Connect™ Real-Time PCR Detection System 8/18
CFX96TM Real-Time PCR Detection System
Dx Real-Time System
www.geneproof.com

Manual © GeneProof a.s. 9/18
www.geneproof.com

CFX Connect™ Real-Time PCR Detection System 10/18
CFX96TM Real-Time PCR Detection System
Dx Real-Time System
www.geneproof.com

Manual © GeneProof a.s. 11/18
www.geneproof.com

CFX Connect™ Real-Time PCR Detection System 12/18
CFX96TM Real-Time PCR Detection System
Dx Real-Time System
www.geneproof.com

Manual © GeneProof a.s. 13/18
www.geneproof.com

CFX Connect™ Real-Time PCR Detection System 14/18
CFX96TM Real-Time PCR Detection System
Dx Real-Time System
www.geneproof.com

Manual © GeneProof a.s. 15/18
www.geneproof.com

CFX Connect™ Real-Time PCR Detection System 16/18
CFX96TM Real-Time PCR Detection System
Dx Real-Time System
www.geneproof.com

Manual © GeneProof a.s. 17/18
www.geneproof.com

GeneProof a.s.
Vídeňská 119 / CZ-619 00 Brno / [phone] / [e-mail]
VERSION: MP_04_15_01 Valid from: 04-06-15 Public 18/18
www.geneproof.com
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