Genfine Purifier HT User manual

GENFINE
Purifier HT
User manual V2.1

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Contents
Chapter 1 Product Introduction1.......................................................................................................3
1.1 Product Name.....................................................................................................................3
1.2 Model ................................................................................................................................3
1.3 Intended use.......................................................................................................................3
Chapter 2 Function Introducion ........................................................................................................4
2.1 Main Components...............................................................................................................4
2.1.1 Front View.............................................................................................................4
2.1.2 RearView ...............................................................................................................4
2.2 Working Principle...............................................................................................................5
2.3 USB Port.............................................................................................................................5
2.4 Consumables .....................................................................................................................5
Chapter 3 Installation ......................................................................................................................6
3.1 Delivery Check ..................................................................................................................6
3.1.1 Unboxing.................................................................................................................6
3.1.2 Integrity Check.........................................................................................................6
3.2 Environment Requirements................................................................................................6
3.3 Precautions........................................................................................................................6
3.4 Installtion Settings...............................................................................................................7
3.4.1 Power Connection....................................................................................................7
3.4.2 Operation Check ......................................................................................................7
Chapter 4 Instructions for Use...........................................................................................................8
4.1 Starting................................................................................................................................8
4.2 Programs .............................................................................................................................8
4.2.1 Home Screen...........................................................................................................8
4.2.2 Program Running....................................................................................................8
4.2.3 New Pogram Creation.............................................................................................9
4.2.4 User Settings ......................................................................................................12
4.2.5 UV Light Settings.................................................................................................12
4.3 Shutting Down .................................................................................................................12
4.4 Emergencies.....................................................................................................................13
Chapter 5 Care and Maintenance ....................................................................................................14
5.1 Instrument Maintenance....................................................................................................14
5.2 Cleaning...........................................................................................................................14
5.3 Magnetic Rods Cleaning..................................................................................................14
5.4 Waste Disposal.................................................................................................................14
5.5 Decontamination Procedure ............................................................................................14
5.5.1 Decontamination reagents Types...................................................................14
5.5.2 Decontamination Procedure ................................................................................15
5.6 Packaging for repair..........................................................................................................15
5.7 Service Contract...............................................................................................................15
5.8 System Log ......................................................................................................................15
5.9 Instrument Disposal.........................................................................................................15
Chapter 6 Technical Parameters......................................................................................................17

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6.1 General Parameters..........................................................................................................17
6.2 Performance Parameters ..................................................................................................18
6.3 Safety Parameters.............................................................................................................18
6.4 Compliance......................................................................................................................19
6.5 Error code.........................................................................................................................19
Chapter 7 Other Information...........................................................................................................20
7.1 Product Date.....................................................................................................................20
7.2 Service life.......................................................................................................................20
7.3 Components List..............................................................................................................20
7.4 Interpretation of graphics.................................................................................................21
7.5 Manual revision date........................................................................................................21
7.6 Target customers..............................................................................................................21
7.7 More information.............................................................................................................21
7.8 Safety and User Guide .....................................................................................................21
Chater 8 Manufacturer and Medical Device Information...............................................................22
Chater 9 Order Information.............................................................................................................22
9.1 Purifier HT confiuration...................................................................................................23
9.2 List of Accessories and Consumabes...............................................................................23
9.3 List of Kits .......................................................................................................................23
Appendix A Certificate of Decontamination...................................................................................24
Appendix B System Log .................................................................................................................25

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Chapter 1 Product Introduction
1.1 Product Name:
Purifier HT Automatic nucleic acid extractor
1.2 Model:
Purifier HT
1.3 Intended Use
Purifier HT （ Figure 1-1）adopts the magnetic beads separation technology to achieve the
collection, release, transfer and incubation of the magnetic beads through the movement of the
magnetic rods and the magnetic tip combs. And by this, it can complete the entire nucleic acid
extraction process.
The instrument can be applied for the extraction and purification of nucleic acid from
different clinic samples. Purifier HT is especially used on research and tests by the personnel who
have been trained professionally. It is used for automatic transfer and processing of magnetic
beads in deep well plates. It is recommended that good laboratory practice (GLP) should be
followed to ensure the reliability of the analysis. Refer to Chapter 6: Technical Parameters.
Figure 1-1. Purifier HT

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Chapter 2 Function Introduction
2.1 Main Components
2.1.1 Front View
Figure 2-1.Purifier HT Front view
2.1.2 Rear View
Figure 2-2. Purifier HT Rear View

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Purifier HT (Figure2-1), The magnetic tip combs matches the deep-well Plate. The magnetic
head consists of two vertically moving platforms. One is used for the magnetic tip rods (24 or 96)
and the other is used for grasping the magnetic tip comb. During running, the magnetic tip comb
and the magnetic rods move vertically to complete the mixing of reagents and the adsorption and
release of magnetic beads.
There are 8 plate positions on the rotary table. Up to 8 pieces of 96/24 deep-well plates can
be placed simultaneously. Prior to the start of program, samples and reagents are loaded into deep-
well plate according to the extraction protocol. The magnetic tip comb is placed on the correct
deep-well plate and the deep-well plate is placed in the corresponding plate position on the rotary
table. During working, the base board start rotating and drive the corresponding deep well plate to
the right place which under the magnetic rods. The magnetic tip comb can be automatically loaded
onto the moving platform.
The door must be closed during operation. It can protect the sample from environmental
contaminants.
2.2 Working Principle
PurifierHT automatic nucleic acid extractor adopts magnetic beads separation technology (Figure
2-3). Different from other methods of magnetic bead purification for liquids transfer, this method
transfers the magnetic beads between deep-well plates containing specific reagents, and the magnetic rods
are loaded with a disposable magnetic tip comb. Through the separation of the magnetic rods and the
magnetic magnetic tip comb, the magnetic beads can be collected, released, transferred and incubated. It
can extract high quality nucleic acid from saliva, swab, dry blood spots, whole blood, serum, plasma,
animal and plant tissues. samples.
Figure 2-3. Diagram of working principle of the instrument
2.3 USB Port
There is a USB port on the front panel for importing and exporting programs.
2.4 Consumables
See "Accessory and Consumables List" for details and ordering information about Purifier
HT's consumables, such as deep-well plates, magnetic tip comb, and HEPA filters.

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Chapter 3 Installation
3.1 Deliver Check
3.1.1 Unboxing
Move the packaged instrument to the operation site. To prevent condensation of water vapor, the
instrument should be left in its protective, anti-static plastic packaging until the ambient
temperature is reached room temperature. Open Purifier HT and accessories carefully. Remove the
instrument from the package and place it on a horizontal surface.
Caution Be careful not to touch or loosen screws or parts specially specified in the
instruction manual. Otherwise, it may result in misplacement and invalidate the
instrument warranty.
Warning Purifier HT weighs about 45kg, if transporting without package, please
handle carefully. It is recommended that two people move the equipment together
and take appropriate precautions to avoid injury.
Please keep the original packing materials for future transportation. The packing is designed to
ensure safe transportation and reduce damage in transit. The use of alternative packaging materials
may not achieve the effect. All instrument related documentation and accessories provided by the
manufacturer are retained for future use.
3.1.2 Integrity check
Check the integrity of the goods. Please check the complete documents and accessories with the
instrument according to the packing list. Visually check the shipping packages, instruments and
accessories for possible transportation damage. If any parts are broken, please contact the
manufacturer in time.
3.2 Environment Requirement
When installing Purifier HT, avoid placing it in the places where there is a lot of dust, vibration,
strong magnetic field, direct sunlight or ultraviolet radiation, airflow, high humidity or high
temperature fluctuation.
Please place the instrument on a regular experiment table to ensure that:
• The working area is flat, dry, clean and secure, with extra space for accessories, cables, etc;
• At least 10 cm of free space is provided for ventilation on the table around the instrument;
• The ambient air is clean, free from corrosive steam, smoke and dust;
• Ambient temperatures range from 5°C (41°F) to 40°C (104°F);
• Humidity is low and condensation does not occur (relative humidity is between 10% and 80%);
• Install the Purifier HT in a protected place where no one can step on or trip over the power cord,
and where it is easy to access the power cord when the plug needs to be removed;
•Care should be taken not to operate the instrument in an environment with potentially harmful
liquids or gases.
3.3 Precautions
• Always ensure that the laboratory power supply voltage meets the specification on the label on the
back of the instrument (Figure 2-2);
• Do not smoke or eat or drink while using this device;
• Clean hands thoroughly after handling the buffer solution;
• Follow regular laboratory procedures for handling samples that may be dangerous;
• Adhere to good laboratory practices and wear appropriate protective clothing such as disposable
gloves and laboratory coat;
• Ensure that the working area is well ventilated;
• Do not spill liquid inside or outside the device.
Caution
Do not place the instrument near magnetic tapes, computer discs or other
magnetic storage devices, such as credit cards, as they may be damaged by the
strong magnetic field of the instrument magnetic head.

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Warning Do not put the Purifier HT magnetic head near the computer monitor, as
this may damage the monitor.
The product has a strong permanent magnet. This product should not be
used by people wearing pacemakers or metal prostheses. Prosthetic devices
such as pacemakers or prostheses may be affected or damaged by close
contact with strong magnetic fields.
3.4 Installation Settings
This section describes the installation Setting that must be performed prior to operating or
relocating the instrument.
3.4.1 Power Connection
Warning
Make sure that the power switch on the rear panel (Figure 2-2) is in the
"O" position. Never connect an ungrounded power outlet. Do not use any power
cord other than the one provided by the manufacturer.
First connect the power cord to the power connector and insert into the instrument (Figure 3-
1). Next, connect the power supply to a properly installed, well-grounded power outlet.
Figure 3-1. Power cord connector
3.4.2 Operation Check
First turn on the instrument to "I". Instruments are initialized for testing and adjusting. The
display interface is the user management interface. It is recommended that have a check,
which use the maintenance program to verify proper instrument operation. If the check is
correct, you can continue to run on your own.

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Chapter 4 Instructions for Use
4.1 Starting
Before turning on Purifier HT, make sure that the voltage on the label which is on the left bottom of
the back panel (Figure 2-2) matches the local voltage.
Warning Never operate the instrument on an ungrounded electrical outlet.
4.2 Programs
4.2.1 Home Screen
The default program displays a list of programs that are preset in the Purifier HT software and cannot
be edited or deleted. The user program is the program that the user edits and saves themselves, and
the user can edit and delete the user program according to the experiment needs. New program can
edit new programs. New programs can also be edited.
Display the current status of the instrument，“Empty”means the instrument is idle
condition，“Run”indicates that the instrument is running a program，“Check” indicates that
the instrument is in the state of power-on self-test.
4.2.2 Program Running
Click "User Program" to enter the program classification interface.
Users can select any file in the table, for example by clicking the "DNA" button to enter the list of
programs. Click the program you want to run, and click "Select" to enter the program details
interface.
The program detail interface displays the step number, board position number, item name, time,
volume, temperature and other parameters. After confirming that the program is correct, click
"Run" to run the program.

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During the program running, the interface displays the total time and remaining time of the program.
Click "Pause" to pause the program.
Click “Continue” to resume running.
Click "Cancel", a dialog box will indicate whether to exit the current running program or not. Click
"Cancel" to continue running, and click "Confirm" to stop running. Customers should use this
function with caution.
4.2.3 New Program Creation
Click "New Program" on the main interface to create a new programs according to the customer’s
requirements. Enter the program name and select the program type. Click or “Back” to
return to the main screen. Click "Next" to enter the program editing interface.