Gilson 155 User manual

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Gilson 155/156
UV/VIS Detectors
User’s Guide
LT1034/©2004 Gilson, Inc. All rights reserved March 2004
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Table of Contents
Declaration of Conformity
1 Introduction
Description ............................................................................ 1-1
Optics .............................................................................. 1-2
Display ............................................................................ 1-2
Features ........................................................................... 1-2
Unpacking............................................................................. 1-3
Customer Service ................................................................. 1-4
Technical Specifications ..................................................... 1-5
Modes and Their Parameters ............................................ 1-9
Single Wavelength Mode ............................................. 1-9
Dual Wavelength Mode ...............................................1-9
Scan Wavelength Mode ............................................... 1-9
Status Mode .................................................................... 1-9
Setup Mode .................................................................... 1-9
File Mode ........................................................................ 1-9
2 Installation
Operating Environment ..................................................... 2-1
Temperature ................................................................... 2-1
Contaminants ................................................................ 2-1
Ventilation ...................................................................... 2-1
Condensation ................................................................. 2-1
Electrical Setup..................................................................... 2-2
Rear Panel Diagram ..................................................... 2-3
Essential Setup ............................................................... 2-4
Optional Connections .................................................. 2-6
Unit ID and Baud Rate Selection ............................... 2-10
Unit ID ............................................................................ 2-10
Baud Rate ....................................................................... 2-11
Plumbing Setup .................................................................... 2-12
Inlet Tubing .................................................................... 2-12
Outlet Tubing .................................................................2-13
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3 Operation
Operation Flowchart........................................................... 3-2
Front Panel Diagram ........................................................... 3-4
Software Structure .............................................................. 3-6
Mode Selection ............................................................... 3-6
Parameter List ................................................................ 3-6
Start Up ................................................................................. 3-12
Wake-Up Displays ........................................................ 3-12
Selecting the Mode ........................................................3-14
Error Messages ............................................................... 3-15
Detector Setup Parameters ................................................ 3-16
First Display For Setup Mode ..................................... 3-16
Second Display For Setup Mode ................................ 3-17
Third Display For Setup Mode ................................... 3-18
Fourth Display For Setup Mode .................................3-19
Single Wavelength Mode ................................................... 3-20
Display For Single Wavelength Mode .......................3-20
Output Channels........................................................... 3-22
Example Run .................................................................. 3-23
Dual Wavelength Mode ..................................................... 3-25
First Display for Dual Wavelength Mode................. 3-26
Second Display for Dual Wavelength Mode ...........3-27
Output Channels........................................................... 3-29
Example Run .................................................................. 3-29
Scan Wavelength Mode ...................................................... 3-32
First Display for Scan Wavelength Mode ................. 3-33
Second Display for Scan Wavelength Mode ............ 3-35
Third Display for Scan Wavelength Mode ............... 3-36
Output Channels........................................................... 3-36
Remote Event/Zero ...................................................... 3-37
Scan Mode Event Marker ............................................ 3-37
Zero Scan ........................................................................ 3-37
Discrete Sample Scan ................................................... 3-38
Example Run .................................................................. 3-39
Programming the Detector ................................................ 3-42
Remote Event ................................................................. 3-43
Remote Zero ................................................................... 3-43
Programming Tools ...................................................... 3-44
Example 1: Startup File ................................................3-45
Example 2: Programmed Run .................................... 3-48
Example 3: Linking to Another File ........................... 3-56
Mobile Phase Tips ................................................................ 3-57
Degas Solvents and Buffers ......................................... 3-57
Check UV and Visible Absorbance of Solvents ....... 3-57
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4 Maintenance
Flow Cell Maintenance ....................................................... 4-2
Cleaning the Inside of the Flow Cell (Analytical,
Preparative, Microbore, and SFC only) .................... 4-2
Unclogging the Flow Cell ............................................ 4-5
Replacing Tubing and/or the Flow Cell ................... 4-5
Lamp Maintenance ............................................................. 4-22
UV Lamp Maintenance ............................................... 4-22
Visible Lamp Maintenance .......................................... 4-29
Fuse Replacement ................................................................ 4-35
5 Troubleshooting
Troubleshooting ................................................................... 5-2
Detector Not Functioning ............................................ 5-2
A Lamp Fails to Ignite/A Lamp Fails While On..... 5-2
Status Lights Blinking/Screen Blank or Dark ......... 5-2
Baseline Spikes ............................................................... 5-3
Baseline Noise ................................................................ 5-4
Baseline Drift .................................................................. 5-5
Autozero Out of Range ................................................ 5-6
Out of Range .................................................................. 5-6
Peaks Too Broad ............................................................ 5-7
Decreased Response to Known Sample .................... 5-7
Abnormally High System Pressure ............................ 5-7
Diagnostics ............................................................................ 5-8
Numeric Analyses on the 155 Detector .................... 5-8
Repair and Return Policies ................................................ 5-10
Telephone Numbers ...................................................... 5-10
Before Calling Us ........................................................... 5-10
Warranty Repair ........................................................... 5-10
Non-Warranty Repair .................................................. 5-10
Rebuilt Exchange ........................................................... 5-11
Return Procedure .......................................................... 5-11
AppendixA Defaults and Limits
Setup Mode ........................................................................... A-1
Single Wavelength Mode ................................................... A-1
Dual Wavelength Mode ..................................................... A-2
Scan Mode............................................................................. A-2
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Appendix B Mode-Specific Functions
Mode-Specific Output Channels ...................................... B-1
Mode-Specific Remote Event and
Autozero Functions ............................................................. B-1
Appendix C Sensitivity
Appendix D GSIOC Control
GSIOC Commands .............................................................. D-2
FIFO Commands .................................................................. D-2
GSIOC Command List ........................................................ D-2
FIFO Command List ............................................................ D-11
Appendix E Replacement Parts
Lamp Assemblies ................................................................. E-1
Cell/Accessory Kits ............................................................. E-1
Flow Cell Assemblies ........................................................... E-2
Inlet and Outlet Tubing ...................................................... E-2
Miscellaneous ....................................................................... E-4
Appendix F DisplayAdjustment
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Application of Council Directives:
89/336/EEC, 73/23/EEC
Standards to which Conformity is Declared:
EN61326, EN61000-3-3, EN61000-3-2, EN61010-1:2001
Manufacturer’s Name ........................................... Gilson, Inc.
Manufacturer’s Address ....................................... 3000 W. Beltline Highway
Middleton, WI 53562
EC Office Address .................................................. Gilson S.A.S.
19 Avenue des Entrepreneurs, B.P. 145
F-95400 Villiers-le-Bel, France
Type of Equipment ................................................. Laboratory Equipment
Model. ....................................................................... 155 UV/VIS Detector
Beginning with Serial Number: 109F8G001
Month and Year of Manufacture: June 1998
Place: Middleton, WI (USA) Michael Jacquart
Issue Date: August 1998 Senior Vice President
Corporate Technology Development
I, the undersigned, hereby declare that the equipment
specified above conforms to the above Directives and Standards.
Declaration of Conformity
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Application of Council Directives:
89/336/EEC, 73/23/EEC
Standards to which Conformity is Declared:
EN61326, EN61000-3-3, EN61000-3-2, EN61010-1:2001
Manufacturer’s Name ........................................... Gilson, Inc.
Manufacturer’s Address ....................................... 3000 W. Beltline Highway
Middleton, WI 53562
EC Office Address .................................................. Gilson S.A.S.
19 Avenue des Entrepreneurs, B.P. 145
F-95400 Villiers-le-Bel, France
Type of Equipment ................................................. Laboratory Equipment
Model. ....................................................................... 156 UV/VIS Detector
Beginning with Serial Number: 109C9H001
Month and Year of Manufacture: March 1999
Place: Middleton, WI (USA) Michael Jacquart
Issue Date: April 1999 Senior Vice President
Corporate Technology Development
I, the undersigned, hereby declare that the equipment
specified above conforms to the above Directives and Standards.
Declaration of Conformity
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1-1
1
Introduction
Description
The Gilson 155 and 156 UV/VIS Detectors are microprocessor-controlled absorbance
detectors that provide dual wavelength detection. Both detector models are compatible
components of a Gilson modular HPLC system. And, the 155 Detector with its front panel
control is also compatible with other commercially available HPLC systems.
The 155 Detector can be controlled via its front panel or via external software, such as
Gilson UniPoint™ System Software. The 156 Detector must be controlled via external
software since it does not have an integral front panel display. For information on how to
control the 156 Detector, refer to the user’s guide for the controlling software.
Gilson 155 UV/VIS Detector
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1-2
Introduction 1
Description
Optics
Gilson’s optical design lets you monitor absorbance
at any wavelength from 190 to 700 nm.
The detector has a sensitivity range of 0.001 to 2.0
AUFS (Absorbance Units, Full Scale).
Display
Gilson designed the 155 Detector software to be
completely self-guiding.
All parameters, values and commands appear on
the four-line display.
All of your options are displayed above the six soft
keys. Press a soft key to choose and carry out a
command. Numeric entries are made using the
keypad.
Features
You can choose from the following modes to
specify detection conditions and basic operating
parameters:
• single wavelength • file
• dual wavelength • setup
• scan wavelength • status
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1-3
Introduction 1
Unpacking
Unpacking
Unpack the detector and its accessories carefully
from the carton.
Open the detector door and remove the foam insert
from inside the detector.
Cross-check the contents against the checklist below
and against your packing slip, to verify that all parts
are included and undamaged. Do this now, even if
the detector will not be used immediately. Many
carriers must receive concealed damage claims
within seven days of delivery.
The detector is shipped with its flow cell, lamps,
and inlet/outlet tubing already installed. The
carton should also contain:
• power cords
• fuse drawers and fuses
• two Pomona recorder cables
• autozero/event cable
• GSIOC cable
Note: The three black-headed shipping screws in
the bottom of the instrument that secure the optical
assembly during shipment must be removed from
the bottom of the instrument before you use the
detector. Save these screws in case you need to ship
the unit.
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1-4
Introduction 1
CustomerService
Customer Service
Gilson, Inc., and its worldwide network of authorized
representatives provide customers with these types
of assistance: sales, technical, applications, and
instrument repair.
If you need assistance and are in the United
States, contact your Gilson representative or call
the Gilson Customer Service Department, toll-free
at 800-445-7661 or call 608-836-1551. You may also
contact the Gilson Customer Service Department
via its e-mail address: [email protected].
Outside the United States, contact your Gilson
representative if you need assistance. For more
specific contact information, refer to the Gilson web
site at www.gilson.com.
To help us serve you quickly and efficiently, please
see the Before Calling Us information page 5-10.
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1-5
Introduction 1
TechnicalSpecifications
TechnicalSpecifications
Warning: Changes or modifications to this device
not expressly approved by Gilson could void the
warranty.
Note: This device has been tested and found to
comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC commercial
environment. This device generates, uses, and can
radiate radio frequency energy and, if not installed
and used in accordance with the instructions, may
cause harmful interference to radio communications.
Operation of this device in residential area is likely
to cause harmful interference; in which case, you
must correct the interference at your own expense.
You must use shielded cables with this device to
ensure compliance with the Class A FCC limits.
Note: The noise, drift, and sensitivities listed on the
following pages are maximums. Under most
circumstances, you can expect performance that
surpasses these specified limits.
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1-6
Introduction 1
TechnicalSpecifications
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1-7
Introduction 1
TechnicalSpecifications
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1-8
Introduction 1
TechnicalSpecifications
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1-9
Introduction 1
Modes and Their Parameters
The available modes and their parameters are
described below.
Single Wavelength Mode
Wavelength, Sensitivity 1, Sensitivity 2, Peak width
Dual Wavelength Mode
Wavelength 1, Wavelength 2, Sensitivity 1,
Sensitivity 2, Peak width, Ratio multiplier, Ratio
Threshold
Scan Wavelength Mode
Wavelength (to monitor before and after the scan),
Sensitivity 1, Sensitivity 2, Sensitivity 3, Peak width,
Scan range, Scan output time scale, Automatic
scan initiation, Initiation threshold
Status Mode
UV lamp status, UV lamp hours, Visible lamp status,
Visible lamp hours, Wavelength, Event input
Setup Mode
Channel 1 scale and offset, Channel 2 scale and
offset, Channel 3 scale and offset, Lamp alarm,
Event channel(s), Autorange channel(s), Autorange
multiplier, Concentration factor, Lamp saver
File Mode
With file mode, you can store as many as ten
program files to save time and to enhance the
reproducibility of your assays. A program file
instructs the detector to change the detection
conditions and to modify the detection parameters
at specified times during the run.
ModesandTheirParameters
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2-1
2
Installation
Operating Environment
You can use the detector in normal laboratory and cold room environments.
The factors described below will influence the performance of your detector.
Temperature
Changes in ambient temperature can produce noticeable output variations, especially at
higher sensitivities.
Avoid areas which are in direct sunlight for part of the day and those which are subject
to draft; for example, do not place the detector near an open window or doorway.
Contaminants
Smoke, vapors, and acid fumes can cause baseline drift and noise.
Ventilation
You must allow at least 4 inches (10 cm) of clearance behind the detector to permit
exhaust from the cooling fan to escape. Also, do not block the ventilation holes on the
bottom of the detector.
Condensation
The detector has two power controls:
• the front panel LAMP ON/OFF key
• the rear panel MAINS switch
To prevent condensation on optical surfaces, always leave the MAINS switch ON.
Turn the detector on and off using the LAMP ON/OFF key.
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