Gold Standard ThunderBolt User manual

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ThunderBolt®User’sManual
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1. 
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Contents
1.Introduction.................................................................................................................................3
1.1.IntendedUse.................................................................................................................................3
1.2.Certifications.................................................................................................................................3
2.SafetyInformation.......................................................................................................................3
2.1.GeneralSafety...............................................................................................................................3
2.2.ElectricalSafety.............................................................................................................................4
2.3.MechanicalSafety.........................................................................................................................4
2.4.BiologicalSafety............................................................................................................................4
2.5.SafetyLabels.................................................................................................................................4
3.SystemDescription.......................................................................................................................5
3.1.System...........................................................................................................................................5
3.2.SystemDetails...............................................................................................................................5
3.3.ComponentDetails.......................................................................................................................6
4.ThunderBoltSoftware..................................................................................................................7
4.1.InstrumentGraphicalUserInterface(GUI)...................................................................................8
4.2.OtherSoftwareApplications.........................................................................................................9
5.RunningaWorklist.....................................................................................................................10
5.1.WorklistPreparation...................................................................................................................10
5.2.SamplesTab................................................................................................................................11
5.3.MicrotiterPlatesTab...................................................................................................................13
5.4.RacksTab.....................................................................................................................................14
5.5.WorklistTab................................................................................................................................14
6.ToolsTab....................................................................................................................................16
6.1.Tools............................................................................................................................................16
6.2.PlateReading..............................................................................................................................16
6.3.AlignmentandCalibration..........................................................................................................17
7.Maintenance..............................................................................................................................20
7.1.DailyMaintenance......................................................................................................................20
7.2.WeeklyMaintenance..................................................................................................................21
7.3.MonthlyMaintenance................................................................................................................21
7.4.PeriodicMaintenance.................................................................................................................22
7.5.Storage,Transportation,andDisposal........................................................................................22
8.LaboratoryInformationSystemIntegration................................................................................23
8.1.LISRecords..................................................................................................................................23
8.2.LISQueryandTransmissionExamples........................................................................................24
8.3.LISConfiguration.........................................................................................................................25
8.4.RS‐232PinLayout.......................................................................................................................26
9.TroubleshootingGuide...............................................................................................................27
10.ContactInformation...................................................................................................................31
10.1.DeviceManufacturer..........................................................................................................31
10.2.AuthorizedRepresentative.................................................................................................31
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1. Introduction
Thepurposeofthismanualistoidentifytheinstrumentcomponentsandprovideinstructionsforuseof
theThunderBolt.Thismanualprovidesbasicsafetyinformation,instructionsforuseandmaintenance,
aswellasgeneraltroubleshootingguidance.Itisrecommendedthatthismanualbekeptnearthe
instrumentandaccessibletotheuseratalltimes.
Note:AllreferencestoCLIA(ChemiluminescentImmunoassay),luminescence,orRLU(RelativeLight
Units)measurementareonlyavailableforusewithThunderBoltinstrumentsequippedwiththe
chemiluminescenceoption.
1.1. IntendedUse
TheThunderBoltisdesignedtoautomatetheprocessingofenzymeimmunoassay(EIA)and
ChemiluminescentImmunoassay(CLIA)tests.Thesystemisonlyintendedtobeusedbyproperly
trainedpersonnel.
TheintendedusefortheThunderBoltisasadiscretephotometricchemistryanalyzerintendedto
duplicatemanualanalyticalproceduresbyperformingvariousstepssuchaspipetting,heating,and
measuringcolorintensityautomatically.TheThunderBoltisalsointendedtoperformasan
‘accessory’tobeusedwithadevicetoenablethatdevicetobeusedinaccordancewithitsintended
purpose.
1.2. Certifications
TheThunderBoltisregisteredandcertifiedashavingmetthefollowingregulatoryrequirements:
ISO13845:2003
ENISO13485:2012
DirectiveEN98/79/EC
EN61010‐1:2001
EN61010‐1:2001‐02
USandCAnationaldeviationsasshowninthetestreport586125.01
2. SafetyInformation
ThefollowingsafetyinstructionsaretobeobservedatalltimesduringtheoperationoftheThunderBolt.
Itisstronglyrecommendedthatallfirsttimepersonnelreadthismanualpriortoworkingwiththe
instrumentandreceivetrainingfromGoldStandardDiagnostics(GSD)oraGSDdistributor.
TheThunderBoltisdesignedandmanufacturedinaccordancewiththesafetyrequirementsforelectronic
andmedicalsystemslistedinthecertificationssectionabovetoensurethattheinstrumentfunctions
safely,bothelectricallyandmechanicallyundernormaluseconditions.TheThunderBoltissuppliedina
conditionthatallowsforsafeandreliableoperation.
2.1. GeneralSafety
TheThunderBoltmustonlybeoperatedinaccordancewiththestatedintendeduse.Itis
recommendedthattheThunderBoltisusedonlywiththesparepartsandaccessoriessuggestedor
providedbyGoldStandardDiagnostics.Theuseandmaintenanceactivitiesdefinedinthismanual
areintendedtoensurethesafetyoftheoperatorandtheproperfunctionalityoftheinstrument.All
systemsurfacesmustbedrywhileoperatingtheThunderBolt.GoldStandardDiagnostics

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recommendsthatalloperatorsbetrainedingoodlaboratorypracticesandobservegenerallaboratory
safetyguidelines.
2.2. ElectricalSafety
TheThunderBoltmustbeoperatedusingapowersourcewithanoperatingvoltagecompatiblewith
therequirementsstatedonthedevicelabel.TheThunderBoltistobeusedonlywiththeprovided3‐
pronggroundingtypeplugtoconnecttheinstrumenttothemainpowersupply.Itisimportantto
ensurethepowerswitchisintheOffpositionpriortoconnectingtheThunderBolttothemainpower
supply.
Theuseofamultiplugisnotallowedanditisrecommendedthattheinstrumentberunfroma
dedicatedsocket.Useonlyextensioncableswithaprotectiveconductorandgroundedcontact.The
ThunderBoltusestwofuses,oneACfuse(partnumber5015)andoneDCfuse(partnumber5383).
Fusesthatarenon‐functioningmustbereplacedusingfuseswhichmatchthevalues(nominalvoltage,
nominalcurrent,andtype)specifiedfortheinstrument.AspareACfuseisincludedwiththe
instrument.
Ifatanytimetheinstrumentbecomesunsafetouse,immediatelyswitchitoffanddisconnectitfrom
themainpowersupply.
2.3. MechanicalSafety
InstallationandserviceoftheThunderBoltiscompletedbyatrainedtechniciantoensureaminimized
exposureoftheoperatortomechanicalrisks.ImproperhandlingoftheThunderBoltmaycause
seriousdamagetotheinstrumentorresultininjurytotheuser.Avoidtouchingtheprobeandother
movingpartswhilethesystemisinoperation.Protectivecoversshouldnotberemovedwhilethe
instrumentisonduetopotentialcontactwithmovingparts.Exerciseextremecautionwhenworking
onorneartheperistalticpumpwhenthecoverisnotinplace.Openingsprovidedforventilationare
notmeantasaccesspointsintothesystem.
Ifthecovermustbeopenedduringoperation,verifythatthemovementoftheprobehasstopped
beforereachinginsidetheinstrument.Thisshouldbedonecautiouslyandonlywhenabsolutely
necessary.
2.4. BiologicalSafety
AnypartsoftheThunderBoltthathavecomeintocontactwithsamples/testreagentsaretobe
treatedasbeingpotentiallyinfectious.Someofthegeneralpurposereagentshavethepotentialto
causeirritationoftheskinandmucousmembranes.Itisrecommendedthattheoperatoruse
appropriatepersonalprotectiveequipment(PPE)suchasgloves,labcoat,andeyeprotectionwhile
usingtheinstrument.FordevicesusedinconjunctionwiththeThunderBolt,itistheresponsibilityof
theusertoobservetheinstructionsandwarningsprovidedbythemanufacturerforproperuseof
reagents.
2.5. SafetyLabels
TheThunderBoltislabeledwithuniversalgeneralwarninglabelstoidentifyriskswhichmaybe
encounteredbytheoperator.

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3. SystemDescription
TheThunderBoltisafullyautomatedmicrotiterplateprocessorthatisabletocompletelyperformsample
processingsteps,includingdilutions,dispenses,incubations,shaking,andwashprocesses.The
ThunderBoltalsoprovidesphotometricandluminescentmeasurementandevaluation.TheThunderBolt
iscontrolledbyaWindowsPCsoftwareprogramthatisspecificallydesignedforthesystem.
3.1. System
TheThunderBoltsystemconsistsofaroboticplatformthatperformsprogrammedEIAandCLIAtests
andaLaptopPCwithsoftwarethatenablesautomatedrunningofassaysteps,worklistgeneration,
datamanagement,anddatareduction.
T
hesystemcomponentsareas
f
ollows:
1SystemCover
2LaptopPC(Netbook)
3BarcodeScanner
4IntelligentRack
5ReagentRack
6MicrotiterPlateCarrier
7Probe(withmounted
camera)
3.2. SystemDetails
ItisrecommendedthattheareaforuseandstorageoftheThunderBoltbeaspacededicatedtothe
instrumentwhichisabletoaccommodatethefollowingspecifications.Foroptimuminstrument
performance,theroomtemperatureshouldbecontrolledattemperaturesbetween20°Cand24°C
(68°Fto75.2°F),therelativehumidityshouldbebetween20%and90%(non‐condensing),andthe
environmentshouldberelativelydust‐freeandfreeofexcessivevibration.
InstrumentPowerSpecifications
Voltage:100–265V
Frequency:50–60Hz
PowerConsumption:12Wwithoutincubator,120Wmax
Itisrecommendedthattheinstrumentbeoperatedoffofadedicatedpowersource.Ideallythe
ThunderBoltshouldbeplacednearanoutletandconnectedtoasurgeprotectororan
uninterruptablepowersupply(UPS).
InstrumentDimensionsandWeight
Width:64cm(25.2in)
Depth:57cm(22.4in)
Height:45cm(17.7in)

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Weight:28kg(61.7lb.)
ThetotalbenchspacerecommendedtoaccommodatetheThunderBoltandrelatedequipmentis84.4
cm(33.2in.)widex182.2cm(71.7in.)highx72.2cm(28.4in.)deep.Thelaboratorybenchmustbe
sturdyenoughtosupportthefullweightoftheThunderBoltaswellasadditionalequipment.Expect
thetotalweightoftheThunderBoltandaccessoryequipmenttobeapproximately50kg(110lbs.).
3.3. ComponentDetails
Note:CertainThunderBoltmodelsmaynotcontainallofthesecomponents.
IntelligentSampleRacks
TheIntelligentsampleracksuseautomaticsamplelocationmanagementtotracksampleIDs,
regardlessofloadedsamplepositions.Thethreerackscanaccommodateupto192patientsamples.
Theremovableracksareinterchangeableandcanaccommodate12,13or16x75mmtubes.Custom
racksarealsoavailable.Rackspositionsarenumberedasfollows:Frontrightisrack1,frontleftis
rack2,backleftisrack3.Sampletubepositionsarenumberedineachrackbeginninginthebackleft
cornerandproceedingdowneachcolumnfromtoptobottomandcontinuingacrosstherackfrom
lefttoright.
ReagentRack
Thereagentrackcontains16reagentpositionsandaccepts22to35mmbottles.Customreagent
adaptersarealsoavailable.Thesampleracksandreagentrackarelocatedonaslidingtrayfor
maximumaccessibility.
MicrotiterPlateCarrier
Themicrotiterplatecarriercontainstworeactionmicrotiterplatepositionsandonepre‐dilutionplate
position.
Camera
Theprobe‐mountedcameramonitorsinternaloperationsinreal‐timeandfacilitatesremote
troubleshooting.
ProbeAssembly
TheThunderBoltutilizesasingleprobe,dualneedlesystem;nodisposabletipsareused.Thissystem
iscapableofprecisepickupsanddispensesforvolumesbetween1uland300ul.Thehigh‐precision
micro‐syringeaspirates1ulwithlessthan3%CVacrossanentirereactionplate.Liquiddetectionis
performedviaconductivity,withaminimumdetectionvolumeof50ul.
BarcodeScanner
Thefullyintegratedbarcodescannersupportsthefollowingbarcodetypes:codabar,code39,
interleaved2of5,code93,code2of5,IATAcode2of5,matrix2of5,code11,code128,telepen,
UPCA,UPCE,EAN13,EAN8,MSI,Plessey,RSS‐14,RSS‐14Limited,RSS‐14Expanded,ChinaPost
Code,andPDF417.
Incubator
Theforcedconvectionincubatorheatstoamaximumof45°C.
Reader
TheThunderBolt’son‐boardreaderisanultra‐compact,fullyintegratedLEDspectrophotometer
whichcontainsthestandardwavelengthsof405,450,490,550and630nm.Customwavelengthsare
alsoavailable.

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Photometricrange:0to3.0OD
Spectralrange:400nmto700nm
Readtime:approx.2min/plate
Precision:0to2.000OD:+‐0.003ODorCV<1%,2.001ODto3.000OD:CV<1.5%
Resolution:0.001OD
Linearity:<1%0to2.000OD(typical),<2%2.001ODto3.000OD
ThunderBoltinstrumentsequippedwiththechemiluminescenceoptionalsocontaina
chemiluminescencereaderandcanhandlebothEIAandCLIAassays.
DetectionType:glow
SpectralRange:300nm‐500nm
DarkCount:50countspersecond(standard)
Measuringtime:100ms(adjustable100‐1000ms)
Dynamicrange:6decades(ordersofmagnitude)
OrbitalShaker
Theintegratedorbitalshakerfeaturesano‐spilldesignwhichcloselyreplicatesmanualshaking.The
ThunderBoltcandispenseand/orincubatewhiletheshakerisinmotion.
Amplitude:1mm
Adjustablespeed:upto900RPMs
Capacity:3MTP(2reaction,1predilution)
Voltage:24V
NetbookPC
TheincludednetbookPCisattachedtotheinstrumentviaaUSBconnection.ThePCalsocontains
ethernet,RS232,andadditionalUSBports.PCMinimumrequirementsarelistedbelow:
Processor:1GHz32‐bit(x86)or64‐bit(x64)
Memory(RAM):1GB(32‐bit)or2GB(64‐bit)
Memory(harddisk):20GBavailable(32‐bit)or24GBavailable(64‐bit)
Ports:USB2.0(RS232,Ethernetportsoptional)
Integratedmonitor:DirectX9graphicsdevicewithWDDM1.0driver,800x600resolution
Software
Thepre‐installedEIA/CLIAGraphicalUserInterfacesoftwarerunsontheWindowsbasedNetbook
deviceincludedwiththeinstrument.Thesoftwareprovidestheabilitytocreateandrunworklists,
analyzeresultsfrompreviousworklistruns,generatereportsofworklistresults,andintegrateswith
anyLISthatsupportstheNCCLSLIS1‐AandLIS2‐Aspecifications.
Thesoftwareishighlyconfigurableandincludessupportforaudiblealertsforvariouseventsthat
occurduringtheuseoftheinstrument.Computerspeakersshouldbeenabledatalltimesand
additionalspeakers(provided)arerecommended.
4. ThunderBoltSoftware
TheThunderBoltsystemincludesasoftwarepackagethatisrequiredinorderfortheThunderBoltto
operate.Allsoftwarecomponentsarepre‐installedonthecomputerincludedwiththeThunderBolt
instrument.Note:GoldStandardDiagnosticsperiodicallyprovidessoftwareupdatestodistributorsand
customers.Thismanualdescribesthecurrentversionofsoftware.ContactthelocalThunderBolt
distributorwithanyfurtherquestionsaboutsoftwareversions.

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4.1. InstrumentGraphicalUserInterface(GUI)
TheinstrumentGraphicalUserInterface(GUI)enablestherunningoftestprotocolsthatare
installedontheThunderBolt.Theinstrumentmustbeattachedtoacomputerprogrammedwith
theinstrumentGUIinordertobeoperational.TheinterfacefortheThunderBoltGUIisdesigned
withatitlebarandtabs:
GUIDocumentFolder
AllrelevantdatarelatedtotheThunderBoltGUIislocatedintheThunderBoltEIAdocumentsfolder:
1. WorklistReports:DataforeachrunontheThunderBoltisautomaticallysavedintheWorklist
Reportssubfolder.Worklistreportsincludetwofilesforeachrun:atextfilewiththeworklist
actionlog,andaPDFcopyoftheworklistreport.Thesefilesareautomaticallynamedbythedate
andtimeatwhichtheywererunontheinstrument;additionalnamelabelcanbeaddedbythe
useratthetimeoftherun.Thesefilescanberetrievedatanytime.
2. Worklists:Worklists,labeledwiththesamenameastheWorklistReports,aresavedinthe
Worklistssubfolder.ThesecanbeopenedfromwithintheGUIsoftwareusingtheLoadbutton.
3. Tests:Testfiles(whicharedisplayedintheAddTestdropdownintheGUIsoftware)arestored
intheTestssubfolder.AnytimetheGUIisnotrunning,testfilescanbeaddedto/removedfrom
thisfolderand/ororganizedintosubfoldersifdesired.
4. Errors:Errorsthatareencounteredduringuseoftheinstrumentarenamedbythedateandtime
atwhichtheyoccurandstoredintheErrorssubfolder.
5. ReportTemplates:Anycustomreporttemplates(ifapplicable)arestoredintheReport
Templatessubfolder.
GUIInitialization
1. VerifythatcomputerwithinstalledGUIisattachedtotheThunderBoltinstrument,poweris
connectedtoinstrumentandtocomputer,andinstrumentpowerbuttonisintheonposition.
TurnoncomputerandclicktheGUIicon(ifGUIdoesnotinitializeautomaticallyatcomputer
startup)toinitializethesystem.
2. WaitfortheThunderBolttoinitialize.Theinstrumentrequiresapproximately30secondsto
automaticallymovetoitsstarting,or“home”position.Theinstrumentmustalwaysbeallowed
tocompletethis“homing”uninterruptedbeforebeingused:

Afterhoming,theGUIopens.
GUITitleBar
Thefollowingaredisplayed/accessibleintheThunderBoltGUITitleBar:
1. WashandWasteBottleStatusIcons:Indicatewhichwash/wastesensorsareenabledandthe
liquidlevelofwashandwaste.
2. WorklistStatusIcon:Indicateswhetherinstrumentiscurrentlyrunningaworklistorifthe
instrumentisidle/stopped.
TitleBar→
Tabs
→


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3. TemperatureDisplay:Displaystheinternaltemperatureoftheinstrument.If/whentheincubator
isturnedonduringtherunningofaworklist,temperaturesoftheleftandrightMTPpositionsare
alsodisplayed.
4. Settings:Usedtoselectvarioussoftwareoptions,describedindetailbelow.
5. Power:Usedtoclosethesoftwareandshutdowntheinstrument.
6. About:TheversionoftheinstrumentsoftwarecanbefoundbypressingtheAboutbutton.
7. User’sManual:ApdfoftheinstrumentUser’smanualisaccessiblebypressingthisbutton.
8. TimeDisplay:Displaysthecurrenttime.

GUISettings
ClickingtheSettingsiconontheTitleBarwillbringuptheSettingswindow,usedtoadjustvarious
settingsforrunningtheinstrumentGUI.
Note:GUISettingsarepasswordprotected.Passwordinformationisavailablefromlocaldistributor.
1. InstrumentInformation:Displaysreaderandinstrumentdetails,includingserialnumberandrun
time.
2. Wash/WasteBottles:Allowsusertoturnon/offliquiddetectionofthewash/wastebottles.
BottledetectioniconsaredisplayedintheGUItitlebar.Usercanalsochoosewhetherornot
worklistwillpausewhenthewashbottlesareemptyorthewastebottleisfull.
3. Samples:SamplePipettingoptionsallowusertodecidewhatactionswillbetakenbythe
instrumentwheninsufficientsamplevolumeisdetectedorsampleaspirationfails.Theautomatic
skippingoflow‐volumesamplesisavailable.
Incubationsettingallowsimplementationofanoptionalwarningiftheincubatorisoutofrange
oftheprogrammedtemperatureduringaworklistrun(ifincubatorisusedintheworklist).
4. Racks:AllowstheusertoenabletheReagentLoadingWizard,(awindowwhichpromptstheuser
tocheckthevolumeofeachreagentbeforestartingaworklist),enable/disableintelligentrack
andtubedetection,andsetreagentrack’s(andintelligentracks’,ifapplicable)size/definition.
5. Reports:Allowsusertoselectcustomlaboratoryinformationandlogoforworklistreports.
Optionsforcustomreporttemplatesarealsoavailable.DefaultreporttemplateandGSDlogo
willbeusedonworklistreportsifnochangesaremade.ContactlocalThunderBoltdistributorif
additionalinformationaboutcustomreportoptionsisrequired.
6. LISCommunication:LISconnectionoptionsaresethere(describedinfurtherdetailintheLIS
Configurationsectionofthismanual).
7. LISTestMappings:AssigningofLIStestIDstoinstrumenttestnamesisperformedhere(described
infurtherdetailintheLISConfigurationsectionofthismanual).
8. Sounds:Allowsusertodecidewhichalarmsareenabledandtoselectwhichsoundsareused.
Additionalcustomalarmsounds(asWAVfiles)maybeaddedtothecomputerforselectionhere.
9. Languages:UsercanselectGUIdisplaylanguagehere.
10. TouchScreen:Allowstheusertoturnon/offtheonscreenkeyboardforusewithtouchscreens.
Note:SoftwaremustberestartedbeforesomeSettingsselectionchangesareapplied.TheGUI
indicateswhenanapplicablesettingischangedthatrequiresasoftwarerestartbeforetakingeffect.
4.2. OtherSoftwareApplications
OtherThunderBoltsoftwareapplicationsareavailablefromGoldStandardDiagnosticsforprotocol
designandservicepurposes.Theseareprovidedtoandcanonlybeusedbytrainedservice
technicians.

TB‐0001.IPage10of31
5. RunningaWorklist
Torunaworklistontheinstrument,initializetheGUIasdescribedaboveandfollowthestepsbelow.
5.1. WorklistPreparation
StandardWorklistPreparation:
1. PerformMaintenance:Priortoeachinstrumentuse,verifythatthemaintenancelogisuptodate
andperformdailystartupmaintenanceasdescribedintheMaintenancesectionofthismanual.
Iftheinstrumenthasbeenmoved,probe‐relatedmaintenancehasbeenperformed,orif
different‐sizedrackshavebeenplacedontheinstrumentsincethepreviousalignment,also
performinstrumentalignmentpriortouse.
2. CheckSettings:Besuretoadjust/viewtheGUISettings(describedinThunderBoltSoftware
sectionabove)asnecessarypriortorunningtheinstrument.
3. PrepareKit:Itisessentialthatallpackageinsertinstructionsforreagenthandlingandstorageare
carefullyfollowedforallkitsrunontheThunderBolt.Reagentandsamplepreparationmustbe
performedaccordingtoinstructionsfromtechnicalservice,specifictoeachtest.Priortotheuse
ofreagents,calibrators,controls,orsamples,alsovisuallyverifythattherearenobubblesinthe
bottlesorvials.Airbubblesmustberemoved(acleantransferpipetteortoothpickcanbeused)
beforeplacingvialsontheinstrument.
AdditionalPreparationSelections(Optional):
1. ExcludeStrips:Iftest(s)torunshouldstartfromapositionotherthanthedefaultA1ontheMTP
reactionorpredilutionplate,navigatetotheMicrotiterPlatestabandclicktheExcludeStrips
buttonbelowthedesiredplate(s).Usethe+and–buttonstoselectthenumberofstripsthat
shouldbeskipped:

Forexample,ifthefirst3columnsoftheplateshouldnotbeused,3stripsshouldbeexcluded;
reactionwellswillbeplacedbeginningincolumn4(aftercompletingthenecessarystepstoruna
worklistontheothertabsasdescribedinthefollowingsections):


TB‐0001.IPage11of31
2. PreloadSampleRacks:Priortoorduringaworklistrun,theusercanpreloadrackswithsample
tubes(outsidetheinstrument)tobeusedinfutureworklists.Topreloadracks:
a. OntheSamplestab,clickAddSamples;selectPreloadSampleRack.
b. IntheAddSampleswindowthatappears,specifytheSampleRackID,RackDescription,
andTubesperSample,thenclickAdd.Note:RackIDmustbeintheformatX‐XXXX.The
firstdigitistheracktype(1fora12mmrack,2fora16mmrack,or4fora13mmrack).
The4digitsafterthedashareusedtodifferentiatetheracks(theuniquenumbercan
rangefrom0000to9999).
c. AddsamplestotherackusingoneormoreoftheSampleEntryMethodsdescribedinthe
followingsection.ClickDonewhencomplete.
d. Repeatthesestepsforeachpreloadedrack.Racksarestoreduntilremoved.
e. Toremovearackfromthestoredlist:ClickAddSamples,selectPreloadSampleRack.
ClicktheRemovePreloadedRacksbutton.Selecttherack(s)toremovefromthelist
(selectionishighlighted)andclickRemove.
5.2. SamplesTab
Thistabisusedtoselectsamplesandtestsfortheworklist.Thefollowingstepsaretakento
completethistab:
1. Addtests:Selectthedesiredtest(s)fromtheAddTestdropdownlist.Testnameswillappear
listedfromlefttorightonthescreenastheyareadded.
a. TestProperties:ClickoneachtestnametoopentheTestPropertieswindow.Enterthe
lotnumber,expirationdate,andappropriateruntimevariables(includingcalibrator
valuesandcontrolranges)asobtainedfromthekit(boxlabels,insert,andvials).
b. MultipleTests:Multipletestscanbeaddedifsettingsarecompatible;onlycompatible
testswillbeavailableintheAddTestsdropdownmenuoncethefirsttestfilehasbeen
chosen.
c. TestFiles:NewassayscanbeaddedtotheAddTestdropdownmenubycopyingand
pastingthetestfilesintotheTestsfolderwithintheThunderBoltEIAdocumentsfolder
beforelaunchingtheGUIsoftware.
d. RemoveTest:Anyaddedtestcanberemovedfromtheworklistbyselectingitinthe
RemoveTestdropdownlist.
2. Addsamples:ClicktheAddSamplesbutton.TheAddSampleswindowwillappearinthe
foreground,andtheRacksviewwillappearinthebackgroundtoallowformonitoringofsample
placementassamplesareloaded:


TB‐0001.IPage12of31
FollowthedetailedinstructionsforsampleplacementasincludedintheAddSampleswindowfor
eachSampleEntryMethodwhileaddingsamples.
ImportantNotes:
IntelligentRacks:Ifintelligentracktubedetectionisenabled,samplescanbeplacedinany
desiredlocationsinthesampleracks.Ifintelligentracktubedetectionisnotenabled,samples
mustbeplacedinthedesignatedblinkingpositions.ThePreloadedSampleRackoptioncanonly
beusedwhenintelligentrackdetectionisdisabled.
TubePlacement:Whileplacingsamples,verifythatsamplesarerecognized(spaceswithloaded
samplesdisplayyelloworblue;unoccupiedspaceswillremainwhiteorgrey).Ifsampletubesare
removedpriortostartingtheworklist(andintelligentracktubedetectionisenabled),theywillbe
removedfromtheworklist,andtheiridentificationwillbelost.Tobeincludedintheworklist,
theymustbeaddedagainusingoneoftheSampleEntryMethodsdescribedbelow.
SampleIDs:SampleIDscannotbeeditedafterplacement.ToeditasampleID,removethesample
fromtheworklistandadditagainwithanewID.DuplicatesampleIDsarenotpermitted;asuffix
willbeaddedtothesampleIDifasamplewithaduplicateIDisadded.
SampleEntryMethods:
a. Manual:Usefornon‐barcodedsampleswithcustomIDs.TypethesampleIDinto
designatedfieldandclickAdd(orpressEnteronthenetbookkeyboard).Placethe
sampleintherackaspromptedandrepeatforallmanually‐labeledsamples.
b. AutoIncrement:UseforAuto‐numberedsamples.ChangetheSampleIDTemplateand
StartNumberifdesiredandclickAdd.Placesamplesinthesamplerack,onebyoneas
prompted.Sampleswillautomaticallybenumberedconsecutively,beginningwith
Sample#1andcontinuingwithSample#2,#3,#4etc.(accordingtoIDTemplateand
StartNumber).
c. Scan:Useforbarcodedsamples(barcodescannerlightwillturnonwhenselected).
ClickAdd.Scanthebarcodedsampletubeapproximately4inches(10cm)infrontof
thebuilt‐inbarcodescanner(oruseahandheldscannerattachedbyUSBifdesired).
Whenthesampleisrecognized,abeepwillbeheard,andthescannerlightwillturnoff.
Placethesampleintherackaspromptedandrepeatforallbarcodedsamples.
Note:Atubemustbeplacedinorderforthescannertoreactivatetocontinueloading
additionalsamples(ifintelligentrackisenabled).
d. Import:UsetoimportsamplelistsfromanExcelspreadsheet.SelectthedesiredXLSor
XLXSfileasprompted.
Note:ThisoptionrequirestheusertofillinanExceldocumenttemplate(Inquirewith
localdistributorforguidelinesofpropertemplateformat)withallsampleIDsandrack
positionslocationspriortoaddingsamplestotheworklist.Samplescanbeloadedinthe
samplerackorina96wellplateintheMTPpredilutionposition.
e. PreloadedSampleRack:Usetoaddpreloadedracks(preparedasdescribedinWorklist
Preparation).ClickAdd.SelectthedesiredrackfromthePreloadedSampleRackslist
andclickAdd.Scantherackaspromptedandplaceitintotheindicatedpositioninside
theinstrument(Scannedbarcodemustmatchtheselectedrackinordertoaddtherack
totheworklist).Repeattoaddadditionalpreloadedsamplerackstotheworklist.
Note:ThisSampleEntryMethodisonlyavailableforusewhenintelligentracktube
detectionisdisabled.

TB‐0001.IPage13of31
Whenallsampleshavebeenadded,clickDonetoexittheAddSampleswindow.Displaywillreturn
totheSamplestab.
3. AdditionalSampleTabOptions(optional):Clickonthesamplenametoselectsample(s)
(selectionishighlighted).UsetheCTRLandSHIFTkeystohighlightmultiplesamples.
a. RemoveSamples:Clickthisbuttontoremovehighlightedsample(s).
b. MultiplySamples:Clickthisbuttontochangethereplicateofhighlightedsample(s).
Usethe+and–buttonstoadjusttheindicatedsamplemultiplication.
c. DragandDrop:SampleswillbepipettedintheorderlistedontheSamplestab.Drag
anddropsamplestoadjustprocessingorderasdesired.
4. QueryLIS(optional):IfLIS‐promptedsampleselectionisdesiredandLISconnectivityhasbeen
correctlyconfigured,clicktheQueryLISbuttonatthistime.LISwillselectappropriatesamples
forthechosentest(s)intheworklist.
5. SelectSamples:IfLIS‐promptedsampleselectionisnotused,clickonthebox(es)nexttothe
samplestoselectthemfortest(s).Checkmarksappeartoindicateselection.
a. Multiplesamplescanbeselectedforatestsimultaneouslybyhighlightingmultiple
samples,thenclickingaboxnexttoasampletoselectallhighlightedsamplesforatest.
b. Toselectallsamplesforatest,rightclickonthedesiredtestnameatthetopofthe
column.Allsamplesinthetestcolumnwillbeselected.
c. Ifthesametestfileisruntwiceinthesameworklist,UsetheSmartFillTestsbuttonto
fillallavailablesamplepositionsonthefirsttestfileandselectremainingsamplestorun
inthesecondtestfileautomatically.
FollowingisanexampleofaSamplestabthathasbeenproperlypreparedforaworklistrun:

Note:RackPositionisindicatedforeachsample(rack#:tubeposition#).
5.3. MicrotiterPlatesTab
Thistabisusedtodeterminethenumberofwells/strips/platesrequiredfortherunbydisplayingall
welllocationsonthetwomicrotiterplates(MTP)andthepredilutionplate.Additionalinformation
abouteachwellisdisplayedonthescreenwhenmousecursorisplacedoverthewell.Thistabalso
includespredilutionplateoptions.Thefollowingstepsaretakentocompletethistab:
1. Verification:VisuallyverifythatalldesiredtestwellsandsamplesappearontheMTPlayoutin
thecorrectplatelocations.
2. Wells:Obtaintherequirednumberofwellsforthetest(s)asindicatedbytheMTPimageand
securethemintoappropriateplateframe(s).
3. PlateFrames:Placetheplateframe(s)withtheappropriatewellsontotheMTPcarrier.
4. AdditionalMicrotiterPlatesTabOptions(optional):

TB‐0001.IPage14of31
a. ExcludeStrips:Thisoption,whichallowsteststostartinplatepositionsotherthanthe
defaultA1position,canonlybeusedifsetduringworklistpreparation,asdescribedin
previoussection.
b. PredilutionPlateImport/Export:Theseoptions,whichallowpredilutionplatestobe
sharedbetweentests,canonlybeusedincombinationwithpredilutionsharingtest
files.Contactlocaldistributorforadditionalinformation,ifneeded.
i. Afterusingaworklisttocreateapredilutionplate,clicktheExportbuttonto
savethepredilutionplateforlateruse.
ii. Touseapreviouslycreatedandexportedpredilutionplate,clicktheImport
buttonandselectthedesiredplate.
iii. Note:Ifimportingapredilutionplate,allsampleswillberemovedfromthe
samplelistandreplacedbythesamplespresentintheplate.
5.4. RacksTab
Thistabdisplaysthereagentrackandsampleracksandisusedtoloadreagents,calibrators,and
controls,andtoverifysampleplacement.Additionalinformationabouteachpositioncanbeseenby
hoveringthemouseoverthedesiredlocation.Thefollowingstepsaretakentocompletethistab:
1. Reagents:LoadreagentsintotheThunderBoltreagentrackusingappropriatelysizedreagent
adaptersandfollowingthecolors/descriptionsindicated.
a. Besurethenotchededgeofeachreagentadapterisfacingforward.
b. Whenaworklistisstarted,theReagentLoadingWizardwillalsoguidetheuserthrough
theprocessofaddingreagents(ifwizardisenabledintheGUISettings).
c. Ifdesired,rightclickonanyreagentbottletosetacustomdeadvolumeforthatbottle.
2. CalibratorsandControls:Placepreparedcalibratorand/orcontrolvialsintotheindicated
locationsinthesamplerack.
a. Calibratorandcontrollocationsareautomaticallyassignedtothefirstavailablerack
position,beginningwiththefrontrightcornerandproceedingtothebackoftherack,
thencontinuingrighttoleftacrosstherack.
b. Whenaworklistisstarted,theReagentLoadingWizardwillalsoguidetheuserthrough
theprocessofaddingcalibrators/controls(ifwizardisenabledintheGUISettings).
3. Samples:Verifytheplacementofallloadedsamples.
5.5. WorklistTab
Thistabdisplaystheinternallivecamerafeed,theworklistactionlog,andtheestimatedruntime.
Thistabisusedtostart,load,save,monitor,pause,stop,andfinishaworklist,andtoshow,export,
andsendresults.Thefollowingstepsaretakentocompletethistab:
1. Name:Enterthenameoftheworklistinthisfield.Ifnonameisentered,worklistwillbe
automaticallynamedwithadateandtimestamp.
2. Operator:Enterthetechniciannameinthisfield.
3. Verification:Verifythatthenumberofsamplesloadedandnumberofteststoruniscorrect.
4. WashBottles:Placeappropriatewashbottlesintheindicatedlocations.Note:WashBottle1is
locatedinfirstwashposition(closesttothefrontoftheinstrument);WashBottle2islocatedin
thesecondwashposition(behindWashBottle1,closesttothewastebottle).
5. Start:PresstheStartbuttontostarttheworklist.

TB‐0001.IPage15of31
a. UsetheSpecialStartbuttontostarttheworklistfromastopped/abortedposition,from
aspecificstepoftheselectedtestfile(s),ortorereadtheplate(s)(platescanonlybe
rereadifworklisthasalreadybeenrun).
6. Check:Checkrequiredreagentandcalibrator/controlvolumesaspromptedbytheReagent
LoadingWizard(ifwizardisenabled).Ifmultiplebottlesofasinglereagentarerequiredto
accommodatetheindicatedvolume,usetheSplitBottleoptionwithinthewizardwindowto
loadadditionalbottle(s).Asplitbottlecanalsoberemovedifdesired,usingtheRemoveSplit
Bottlebutton.
7. Run:Closethelidaspromptedandwaitforteststorun.Worklistactionscanbemonitored
throughouttherunusingthelogfileandthelivecamerafeed.Thebaratthebottomofthescreen
estimatesworklistruntime.
a. Itisimportanttokeepcoverclosedfortheentiredurationoftheworklist,especiallywhen
usingthechemiluminescenceoption.
b. Ifnecessary,usethePausebuttontotemporarilypauseaworklistortheStopbuttonto
completelyabortaworklist(doorwillunlockinbothcases).
8. Finish:Aftertheworklistiscompleted,clickOKtostopthealarm(ifendofrunsoundisenabled).
9. Report:ClickShowReporttodisplaytheworklistreportaftertherunhascompleted.
a. ReportOptions:Onceopened,theworklistreportcanbeexportedtovariousfiletypes
and/orprinted/savedtoanotherfileloacationasdesired(Reportinpdfformatissaved
automaticallyintheWorklistReportsfolderbydefault).
b. TestPropertiesOptions:
i. IncorrectValues:Ifusernoticesthatreportdoesnothavethecorrecttest
propertiesvalues,closethereport,navigatetotheSamplestab,clickthetest
name,modifytestpropertiesasneeded,andclickShowReporttodisplaythe
worklistreportwiththeupdatedvalueslisted/includedinthecalculations.
ii. OmittedReplicates:Iftestcontainsmultiplecontrolorcalibratorreplicates,and
userdesirestoomitanout‐of‐rangereplicatefromthecalculations,navigateto
theSamplestab,clickthetestname,clickDropBottles,selectwell(s)toomitfrom
calculations,andclickShowReporttodisplaytheworklistreportwiththe
selectedwell(s)ommitedfromthecalculations.Note:Thisoptioncanonlybe
usediftestfilepermitsreplicatedropping.
10. SendResultstoLIS:ClickSendtoLIStosendworklistresultstotheLISafterrunhascompletedif
LISconnectivityhasbeencorrectlyconfigured.
OtherAvailableWorklistTabOptions:
1. ExportResults:Usethisbutton,ifdesired,toexportresultsdirectlytoanexcelspreadsheet
(SampleIDs,rawdata,andcalculatedresultswillbeseparatedontoseparatesheetsanddisplayed
ina96‐wellformat)forfurtheranalysis.
2. ExportLog:Usethisbutton,ifdesired,toexporttheworklistactionlogtoanotherfilelocation(it
isautomaticallysavedintheWorklistReportsfolderbydefault).
3. New:ClickNewtoclearsamplesandtestsfromthecompletedworklistandallowthesetupofa
subsequentnewworklist.
4. Load:ClickLoadtoopenapreviouslysavedworklist,eithertoobtainworklistinformation/edit
thereportortoreruntheworklist.Alltests,information,samples,andresultsareloadedwhen
apreviouslyrun/savedworklistfileisloaded.Note:Theintelligentrackisdisabledwhenaworklist

TB‐0001.IPage16of31
isloaded(evenifitwaspreviouslyenabledwhenoriginalworklistwasrun).Test/sample
selectionscannotbeeditedwithinaloadedworklist(TestPropertiescanbeedited).
5. Save:ClickSavetosavethecurrentworklistwithanewfilenameortooverwritepreviouslysaved
worklistfilewithanymodificationsmade.
6. ToolsTab
TheToolstabdisplaystheinstrumentfunctionsthatmaybeutilizedoutsideofanormalworklistrun.
Thesefunctionsareavailableforuseanytimethataworklistisnotrunning:

6.1. Tools
1. HomeInstrument:Usethisbuttonto“home”theinstrument,justasisdoneautomaticallyatGUI
startup.
2. ParkInstrument:UsethisbuttontoresettheThunderBoltbacktoitsstarting,or“parked”
position(withtheprobeassemblyabovethewashcup).
3. PrimeInstrument:Inthepopupwindow,selectdesiredwashbottle(s)andnumberofpriming
cyclesandclickStart.Ifbothwashesareselected,thesetnumberofprimingcycleswillbe
performedwithwashbottle1andthenimmediatelysubsequentlyperformedwithwashbottle2.
PrimingcanbeinterruptedusingtheStopbutton.
Note:Donotmoveslidingreagenttrayduringpriming.
4. ManualShake/HeatPlate:Usethisbuttontomanuallyshakeand/orincubateaplate.Select
preferredspeedand/ortemperatureasdesired.UsetheStartandStopbuttonsorsettimerif
desired.
5. SetLightIntensity:UsethesliderbartoadjustdefaultlightintensityoftheinteriorLEDlights.
6. MaintenanceLogs:Clicktoviewdates/timesofallrecentmaintenanceactions(priming,
instrumentandreaderalignment,readercalibration,washpumpcalibration).
6.2. PlateReading
Any96wellplatecanbereadwiththeThunderBoltreader(RLUreadingcapabilityisonlyavailableon
instrumentswiththechemiluminescenceoption).Inthepopupwindow,selectdesiredwellstobe

TB‐0001.IPage17of31
readusingacursortohighlight.CTRLandSHIFTbuttonscanalsobeusedtohighlightspecificwells
oneither/boththeleftandrightplate(s).
Settheapplicableoptionsforthereadtype:
1. ManualReadPlateODs:Setmeasurementwavelengthandreferencewavelength(optional).
2. ManualReadPlateRLUs:Setintegrationtime.
Labelthereaddatausingthecommentfield(optional).Clickstart.Measurementswillappearonthe
screenastheplateisread.Whenreadiscomplete,exportreadingsusingtheExporttoCSVbutton
orprintusingthePrintbutton.Note:ManualreadingsareNOTsavedautomaticallybythesoftware.
6.3. AlignmentandCalibration
Theseinstructionsareintendedtoprovideanoverviewofthesefunctions;detailedstepbystep
instructionsareincludedinpop‐upwindowswithintheinstrumentGUIwheneachfunctionis
performed.
AlignInstrumentandAutoAlignInstrument
Itisrecommendedthattheinstrumentalignmentorautoalignmentbeperformedandrecordedas
apartoftheroutineinstrumentmaintenance.
GeneralInformation
1. CancelledAlignment:Ifanalignmentiscancelled,itisrecommendedthatthealignment
procedurebefullycompletedpriortothenextinstrumentuse.
2. MaterialsRequired:Itisrequiredthatthenecessarycomponentsbeplacedontheinstrument
priortostartingtheinstrumentalignmentprocess.Besurethatalignmentcomponentsare
seatedsecurelybeforestartingthealignmentprocedure.
AlignInstrument:Oneemptysampletubeisplacedinthebackleftpositionofeachsamplerack.
OneAlignmentPlateorMTPisplacedineachofthethreeMTPpositionsontheMTPcarrier.
AutoAlignInstrument:OneAutoAlignmentPlateisplacedineachofthethreeMTPpositionson
theMTPcarrier.Cuvetteisremovedfromthereagentrack(Besuretoremoveallreagentsand
adaptersfromthereagentrackaswell).TheAutoAlignmentPlugisplacedinthecuvetteposition
onthereagentrackwiththecircularpartflushagainsttheclearracktopasshown:

3. ProbeMovement:Theprobeismovedinthealignmentproceduresusingthebuttonsonthe
screenorthealternativekeyboardkeys:
a. Theleftandrightarrowkeysareusedtomovetheprobetotheleftandright(X‐axis).
b. Theupanddownarrowkeysareusedtomovetheprobetowardthefrontandbackof
theinstrument(Y‐axis).
c. ThePageUpandPageDownkeysmovetheprobeupanddown(Z‐axis).

TB‐0001.IPage18of31
Instructions:AlignInstrument:TheuseralignstheXandY‐axispositionsoftheprobewiththe
sampleracksandmicrotiterplates.TheZ‐axisposition,whichmovestheprobeupanddown,isnot
manuallyalignedbytheuser:itisalignedautomaticallybytheinstrumentduringthisprocedure.
1. ClickontheAlignInstrumentbutton.Apopupwindowwillappearandprovideinstructionsfor
eachalignmentstep.
2. Toaligntheprobeateachposition,usethebuttonsorkeystopositiontheprobeneedlesover
thecenterofeachlocationasprompted.Someexamplealignmentpositionsareshownbelow:
a. Washcupalignmentstep:Aligntheprobetothemiddleofthewashcup,thenmoveit
downtoverifythattheprobecanreachthebottomofthewashwellwithoutresistance.
Properalignmentisshownhere:

Note:AfterthealigningthewashcupandclickingNext,theinstrumentwillautomatically
calibratethez‐sensortothebottomofthewashcup.
b. Sampletubealignmentsteps:Alignthecenteroftheprobeneedlestothecenterofthe
sampletube(XandYaxes).Properalignmentisshownhere:

3. Aftercompletingallprobealignmentpositions,removetheAlignmentPlatesorMTPsas
prompted,priortocontinuing.
Note:Ifthesearenotremoved,theZaxispositioncannotbeproperlysetbytheGUI.
4. Atthefinalalignmentstep,ensurethattheprobeiscenteredabovetheleftMTPcarrierposition,
asprompted.Thecorrectplacementoftheprobeisshownbelowwiththetipcenteredbetween
fourholesandroughlyabovetheword“Hot”printedontheincubatorsurface:

TB‐0001.IPage19of31
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5. ClickFinishwhenpromptedtocompleteandsavethealignmentandexitthewindow(instrument
willreturntoitshomeposition).
Instructions:AutoAlignInstrument:Aftertheusersetsthestartingpositionoftheprobeonthe
MTPcarrier,theinstrumentalignsallaxispositions(X,Y,andZ)automaticallyduringthisprocedure.
1. ClickontheAutoAlignInstrumentbutton.Apopupwindowwillappearandprovideinstructions
foreachalignmentstep.
2. AligntheprobeneedlestotheedgeoftheMTPcarrierasprompted.Yaxis(fronttoback)position
oftheprobemustbeexactlyabovetherearcarrierlip.Xaxis(lefttoright)andZaxis(upand
down)positioningarenotimportantatthisstep.Properalignmentisshownhere:

3. Verifyproperplateandplugpositioningwhenpromptedandallowtheinstrumenttocomplete
therestofthealignmentautomatically(Thissteptakesapproximately10‐15minutes).
4. ClickFinishwhenpromptedtocompleteandsavethealignmentandexitthewindow(instrument
willreturntoitshomeposition).
AlignReader
Thereaderalignmentprocessisperformedtoensurethatthereaderisinthecorrectpositionfor
readingMTPs.Itisrecommendedthatthereaderalignmentbeperformedandrecordedasapartof
theroutineinstrumentmaintenance.
GeneralInformation
1. RemoveallmicrotiterplatesandAlignmentPlatesfromtheMTPcarrierpriortostartingthe
readeralignmentprocedure.
2. Thereaderismovedusingthebuttonsonthescreenorthealternativekeyboardkeysaslisted
below:
a. UsetheleftandrightarrowkeystomovetheLEDguidelighttotheleftandright.

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b. UsetheupanddownarrowkeystomoveMTPcarriertowardthefrontandbackofthe
instrument.
Instructions
1. ClickontheAlignReaderbutton.Awindowwillappearandprovideinstructionsforeach
alignmentstep.
2. VerifythatthereaderLEDguidelightisinthecenteroftheholeundertheupperleftA1position
whenprompted.Adjustpositioningasnecessary.
3. Closethecoveroftheinstrumentwhenprompted.
4. WaitfortheinstrumenttocompletetheLEDcalibrationandreaderalignment(Thissteptakes
approximately10minutes).
5. ClickFinishwhenpromptedtocompleteandsavethealignmentandexitthewindow.
CalibrateReader
Readercalibrationisperformedduringmaintenancevisitsorservicecalls,asapplicable(eg:ifreader
isreplaced).Calibrationshouldonlybeperformedbyatrainedservicetechnicianusingacalibrated
plate.ContactthelocalThunderBoltdistributorforadditionalinformation.
CalibrateLuminescenceReader
IftheThunderBoltisequippedwiththechemiluminescencereaderoption,thisportionofthereader
canalsobecalibratedbyatrainedservicetechnician.Calibrationofthechemiluminesencereader
requiresacalibratedplate.ContactthelocalThunderBoltdistributorforadditionalinformation.
CalibrateWashPump
TheWashPumpCalibrationoptionisusedbyservicetechnicianstoverifythatthewashpumpis
functioningproperly.Touse,verifythatwashbufferisloadedintheWash1position,thatacuvette
isinplaceonthereagentrack,andclicktheCalibrateWashPumpbutton.Followinstructionsas
promptedtocalibratethewashpump.

7. Maintenance
Maintenanceproceduresshouldbefollowedtoensureproperinstrumentfunctionality.Itissuggested
thatmaintenanceactivitiesberecordedusingtheprovidedmaintenancelog.Theinformationbelow
describestheroutinemaintenancestepsfortheThunderBoltsystem.
7.1. DailyMaintenance
Dailymaintenanceincludesstartupandshutdownsteps.Startupstepsareperformedpriortothe
firstrunofthedayandshutdownstepsareperformedfollowingthelastrunoftheday.Itis
recommendedthatthestartupandshutdownstepsbeperformedanytimetheinstrumentisidlefor
fourormorehourstopreventbuild‐upinthefluidicslines.
Startup
1. Ensurewashbottle(s)areproperlyconnectedtotheThunderBolt.
2. Primetheinstrumentusingthewashbuffer(s)thatwillbeusedfortestingaminimumof10
primingcyclesperline.
3. Ensurethatallwashbuffersarewithintheirexpirationdatepriortoeachuse.
