Grant-bio DEN-1 User manual

Densitometer
DEN-1
Operating instructions
For version V.1GW

Contents
1 Safety ........................................................................................3
2 General Information ................................................................4
3 Getting started .........................................................................5
4 Operation of DEN-1..................................................................7
5Maintenance .............................................................................9
6Specifications...........................................................................10
7Guarantee and service ............................................................11
Page 2DEN-1
Operating instructions
Version 1.03 - July 2013

1. Safety
The following symbol means:
Caution: Make sure you have fully read and understood the present manual before using the
equipment. Please pay special attention to sections marked by this symbol.
GENERAL SAFETY
Use only as specified in the operating manual provided.
The unit should not be used if dropped or damaged.
After transportation or storage keep the unit under room temperature for 2–3 hrs before connecting it to the
electric circuit.
Use only cleaning and decontamination methods recommended by the manufacturer.
Do not make modifications in design of the unit.
ELECTRICAL SAFETY
Connect only to a external power supply unit with voltage corresponding to that on the serial number label.
Use only the external power supply unit provided with this product.
Ensure that the power switch and external power supply unit are easily accessible during use.
Disconnect the unit from the external power supply unit before moving.
If liquid penetrates into the unit, disconnect it from the external power supply unit and have it checked by a
repair and maintenance technician.
DURING OPERATION
Do not operate the unit in environments with aggressive or explosive chemical mixtures.
Do not operate the unit if it is faulty or has been installed incorrectly.
Do not use outside laboratory rooms.
The Select and Install buttons are used only for calibration of the unit according to p. 3.4. Do not press the
buttons in other cases, as this can cause calibration reset and recalibration will be needed.
BIOLOGICAL SAFETY
It is the user's responsibility to carry out appropriate decontamination if hazardous material is spilt on or
penetrates into the equipment.
Page 3
DEN-1
Operating instructions Version 1.03 - July 2013

2. General Information
Densitometer DEN-1 is designed for solution turbidity measurement in the range of 0.3 -
6 6
5.0 McFarland units (100x10 - 1500x10 cells/ml). DEN-1 is capable of measuring
solution turbidity in a wider range (5.0 - 15.0 McFarland units) however, it is important to
be aware that in this case the standard deviation values increase.
Densitometer DEN-1 is used for determining the concentration of cells (bacterial, yeast
cells) in the fermentation process, for the detection of susceptibility of microorganisms
against antibiotics, for identification of microorganisms with various test systems, for
measuring optical density at fixed wavelength and for quantitative evaluation of
substance concentration.
The operation principle is based on optical density measurement with digital result
representation in McFarland units.
The unit is calibrated at the factory and saves calibration data when switched off.
However, it can be calibrated by 2-6 points in 0.5 - 5.0 McFarland unit range if
necessary. Both commercial standards (e.g. produced by bioMerieux, Lachema, etc.)
and the standards prepared in the laboratory can be used for calibration. Table 1 shows
the data provided by the McFarland standard manufacturer bioMerieux.
Table 1. Interpretation of McFarland Standard results into corresponding numeric values of
bacterial suspension concentration and their optical density at 550 nm.
*Bacterial concentration depends on microorganism size. The numbers represent an
average value valid for bacteria. For yeasts, which are larger in size, these numbers should
be divided by about 30.
**Values correspond to optical densities of bacterial suspensions. The BaSO solutions
4
optical density values differ, because the particle size and form differ from those of
bacteria and light is diffracted differently.
McFarland
Standard
0.5
1
2
3
4
5
Composition
Concentration
BaSO4
-5
2.40 10 mol/l
-5
4.80 10 mol/l
-5
9.60 10 mol/l
-4
1.44 10 mol/l
-4
1.92 10 mol/l
-4
2.40 10 mol/l
Bacterial concentration*
6
150 x 10 cells/ml
6
300 x 10 cells/ml
6
600 x 10 cells/ml
6
900 x 10 cells/ml
6
1200 x 10 cells/ml
6
1500 x 10 cells/ml
Theoretical optical
density at 550 nm**
0.125
0.25
0.50
0.75
1.00
1.25
Interpretation
Page 4DEN-1
Operating instructions
Version 1.03 - July 2013

3. Getting started
3.1 Unpacking
Remove packing materials carefully and retain for future shipment or storage of the unit.
Examine the unit carefully for any damage incurred during transit. The warranty does not
cover in-transit damage.
3.2 The DEN-1 set includes:
Standard set
Densitometer DEN-1 ..............................................................................................1 piece
D16 adapter for tubes with external diameter 16 mm ............................................1 piece
External power supply unit......................................................................................1 piece
Operating Manual; Declaration of Conformity..........................................................1 copy
Optional accessories
DENMCF STDS calibration kit for glass tubes 16 mm in diameter....................on request
DENMCF 18 STDS calibration kit for glass tubes 18 mm in diameter...............on request
3.3. Set up:
Place the unit on the horizontal even working surface.
Plug the external power supply unit into the 12 V socket at the rear side of the unit (fig.1/v).
3.4. Calibration
The device is pre-calibrated at the factory for operation with the glass tubes 18 or 16 mm in
external diameter (see the label on the bottom side of the unit) at temperature range from
Fig.1 Rear panel
Power DC-12 Calibration
select install
Page 5
DEN-1
Operating instructions Version 1.03 - July 2013

+15°C to +25°C and saves calibration data when being switched off.
Note! For work with other type tubes (e.g. with different outer diameter, bottom shape or
different material, e.g. transparent plastic tubes) it is necessary to perform
calibration in the specified tubes.
Perform calibration in the following sequence from the lower calibration value to the higher
values: 0.5, 1.0, 2.0, 3.0, 4.0, and 5.0.
To perform calibration:
3.4.1. Switch ON (position I) the unit with the Power switch (Fig.1/u) on the rear panel.
3.4.2. Press the Select button (fig.1/w) on the rear panel (use a thin pin of maximum diameter 2
mm for pressing the Select and Install buttons). A flashing "0.5" indication will be shown on
the display, showing that the unit is ready to save calibrations value of the first point 0.5 McF.
3.4.4. Press the Install button using a pin (fig.1/x). First point “0.5” will be saved in the unit memory
and the next calibration value will be displayed (flashing 1.0 indication will be displayed).
3.4.5. Repeat steps 3.4.3 - 3.4.4 until the calibration is completed (1-5 times, i.e. as many times as
many points the chosen calibration curve has).
3.4.6. If a standard is not available, press the Select button for the next calibration value to be
displayed.
3.4.7. After installing the last standard value “5.0”
3.4.8. After finishing the calibration switch OFF the unit using the Power switch (position O).
Disconnect the external power supply unit from electric circuit.
3.5
An adapter D16 must be used for work with tubes which have external diameter 16 mm.
Insert the adapter into the tube socket (fig.2/v), perform calibration.
Use at least 2 points for calibration.
Note! Shake the tube with the standard solution, if necessary (it is recommended to use
a vortex mixer, e.g. Personal Vortex V-1 plus, for shaking).
3.4.3. Insert the tube with the standard solution, corresponding to the calibration point value, into
the socket of Densitometer (fig.2/u).
Note! It is recommended to calibrate as many points as possible to obtain precise
results. The minimum requirement is to calibrate 2 points closest to the working
range limits (e.g. 0.5 and 5.0 for operation in 0.5 - 5.0 McF unit range).
or skipping it (the Select button), the unit exit the
calibration mode automatically. The unit is ready for operation.
Note! If pressing the Install button during the calibration process does not cause
switching to the next standard value, it means that the currently inserted in the
densitometer socket standard has lower turbidity value than the previous
standard. The reason is that the inserted standard solution turbidity does not
correspond to the required value (the standard needs to be shaken or replaced).
Installation of adapter D16
Page 6 DEN-1
Operating instructions
Version 1.03 - July 2013

4. Operation of DEN-1
Recommendation during operation
Keeping the unit switched on for 15 min before starting the operation for the operation mode,
stabilisation is recommended.
If flat-bottomed tubes are used, the solution level should be higher than 7 mm from a tube bottom;
if round-bottomed tubes are used, the level should be higher than 12 mm from a tube bottom.
4.1. Connect the external power supply unit to electric circuit.
4.2. Switch ON the unit using the Power switch (Fig.1/u) on the rear panel (position I).
4.3. The following indication may be shown on the display (fig.2/v):
“0.0” means that the unit is calibrated and ready for operation (if no any tube inserted);
“CC” (flashing) means that the unit is not calibrated. Calibrate the unit.
“EE” means that operator error message. Switch OFF the unit and then switch ON again.
4.4. Shake the tube with the solution (it is recommended to use a vortex mixer, e.g. Vortexer PV-1, for
shaking) and insert it into the socket (fig.2/u). The McFarland value for the solution will be
shown on the display (fig.2/v).
4.5. Glass and transparent plastic tubes (16 or 18 mm in external diameter) can be used for work with
DEN-1 densitometer. An adapter must be used for work with tubes which are 16 mm in external
diameter.
Note! The unit must be calibrated each time a tube type (e.g. with different outer diameter,
bottom shape or different material [transparent plastic tubes]) is changed.
4.6. After finishing the operation switch OFF the unit using the Power switch (position O). Disconnect
the power supply from electric circuit.
·
·
-
-
-
Fig.2 Front panel
Page 7
DEN-1
Operating instructions Version 1.03 - July 2013

5. Maintenance
Cleaning
Do not use solvents.
Before using any decontamination or cleaning method except that recommended, check
with our Service Department, or in other countries with our distributor, that the proposed
method will not damage the equipment.
The cases can be cleaned with a damp cloth after disconnection.
Page 8DEN-1
Operating instructions
Version 1.03 - July 2013

6. Specifications
The product is designed for operation indoors in a laboratory at altitudes up to 2000m, with ambient
temperature from +4°C to +40°C and maximum relative humidity 80% for temperatures up to 31°C
decreasing linear ly to 50% relative humidity at 40°C.
·Light source ......................................................................................................................LED
·Wavelength ......................................................................................................λ = 565 ±15 nm
·McFarland unit range .........................................................................................0.3–15.0 McF
·Display resolution........................................................................................................0.1 McF
·Accuracy, of the full scale..................................................................................................+ 3%
·Measurement time ..........................................................................................................1 sec
·Sample volume...................................................................................................2 ml minimum
·Recommended external diameter of tube...............18 mm or 16 mm (when using D16 adapter)
·Display..............................................................................................................................LED
·Dimensions ...................................................................................................165x115x75 mm
·Input current/power consumption...................................................................12 V, 80 mA / 1 W
·External power supply unit ................................ input AC 100–240 V 50/60 Hz, output DC 12 V
·Weight *..........................................................................................................................0.7 kg
* Accurate within ±10%.
Grant is committed to a continuous programme of improvement, specifications may be changed
without notice.
Page 9
DEN-1
Operating instructions
Description
Optional accessories
Adaptor for tubes 16 mm in external diameter
D16
Calibration kit for glass tubes 16 mm in diameter
DEN MCF STDS
Calibration kit for glass tubes 18 mm in diameter
DEN MCF 18 STDS
Description
Replacement parts
Version 1.03 - July 2013

7.1 Guarantee
When used in laboratory conditions and according to these working instructions, this
product is guaranteed for TWO YEARS against faulty materials or workmanship.
7.2 Service
For service, return for repair to our Service Department in the UK or, in other countries,
to our distributor.
7. Guarantee and Service
Page 10 DEN-1
Operating instructions
Version 1.03 - July 2013