Instruction Manual
www.hannainst.com
DearCustomer,
Thank you for choosing a Hanna Product.
Please read the instructions carefully before using the
chemical test kit. It will provide you with the necessary
information for correct use of the kit.
Remove the chemical test kit from the packing material and
examine it carefully to make sure that no damage has
occurred during shipping. If there is any noticeable dam-
age, notify your Dealer or the nearest Hanna office
immediately.
Each kit is supplied with:
•HI 3864A-0 Reagent, packets (100 pcs);
•HI 3864B-0 Reagent, packets (100 pcs);
• 1 checker disc (with 2 discs, LR and HR);
• 2 glass vials with caps;
•1 mirror.
Note: Any damaged or defective item must be returned in
its original packing materials.
HI 3864
Phenols
Test Kit
ISTR3864 11/99 PRINTED IN ITALY
Phenols are widely used in pharmaceuticals, dyes and indica-
tors and as general disinfectants. They may occur in household
and industrial wastewaters and in natural waters; they can
also enter potable water supplies and chlorination of such
waters results in malodorous chlorophenol products that are
detectable from 0.001 mg/L (1 ppb). The HANNA colorimetric
method determines phenol and all ortho and para substituted
phenols. Since substitution generally lowers the response, the
readable value obtained by this method is the minimum
concentration of phenolic compounds present. Natural water
usually contains less than 0.001 mg/L (ppb) of phenols, but
sometimes values up to 0.02 mg/L (20 ppb) also occur.
Since the concentration of phenols in wastewater is generally
subjected to biological and chemical degradation, preserve
samples in the refrigerator and analyze within 4 hours after
collection.
Note: mg/L is equivalent to ppm (parts per million), ppb are
parts per billion.
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONS
SIGNIFICANCE AND USESIGNIFICANCE AND USE
SIGNIFICANCE AND USESIGNIFICANCE AND USE
SIGNIFICANCE AND USE
CHEMICAL REACTIONCHEMICAL REACTION
CHEMICAL REACTIONCHEMICAL REACTION
CHEMICAL REACTION
READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT
• Verify if the mirror and the LR (0÷1 mg/L) disc are
already pre-installed in the checker. If not, install both
as shown in the figure.
INSTRUCTIONSINSTRUCTIONS
INSTRUCTIONSINSTRUCTIONS
INSTRUCTIONS
Phenolic compounds react to form a yellow to orange dye with
4-amino antipyrine in alkaline solution buffered at pH about
10. The absorbance of this colored product is proportional to
the concentration of phenols present in the aqueous sample.
• Fill each glass vial with 10 mL of
sample, up to the mark.
• Insert one of them into the left hand opening of the
checker disc. This is the blank.
• Addtotheother
glass vial 1
packet of HI
3864A-0 Re-
agent. Replace
the cap and
mix.
• Add 1 packet of HI 3864B-0 Reagent.
• Start the waiting time of 10 minutes.
Replace the cap and
shake the vial until the
powder has totally dis-
solved. The solution will
turn a color yellow to or-
ange. This is the reacted
sample.
• Remove the cap and in-
sert the reacted sample
intotherighthandopen-
ing of the checker disc.
• Hold the checker disc so that
a light source illuminates the
samples from the top.
• After the 10 minutes have
passed, rotate the disc while looking at the color test
windows and stop when you find the color match. Read
Range 0 to 1.00 mg/L (ppm) as Phenols LR
0.5 to 5.0 mg/L (ppm) as Phenols HR
Smallest Increment 0.02 mg/L (ppm) Phenols LR
0.1 mg/L (ppm) Phenols HR
AnalysisMethod Colorimetric
Sample Size 10 mL
Number of Tests 100
CaseDimensions 235x175x115 mm (9.2x6.9x4.5")
Shipping Weight 573 g (20.2 oz.)
the value in the result window and record it in mg/L (or
ppm) of Phenols.
• If the color of the sample is too intense to make a color
match with the mirror installed, then remove from the
checkerdisc:
i. the glass vials
ii. the LR disc
iii. the mirror and its holder.
• Installthe HR (0.5÷5mg/
L)disc andreplacethe glass
vials in the checker disc.
• Hold the checker disc so that a
light source illuminates the
samplesfromtheback windows.
• Rotate the disc while looking at
the color test windows and stop
when you find the color match.
Read the value in the result
window and record it in mg/L (or ppm) of Phenols.
Forbest results: Intensely coloredsampleswill make thecolor
matching determination difficult and they should be
adequately treated before performing the test. Sus-
pended matter in large amounts should be removed
by prior filtration.
Caution: Ultraviolet radiation may cause fading of colors.
When not in use, keep the disc protected from light,
in a cool and dry place.
The chemicals contained in this kit may be hazardous if
improperly handled. Read Health and Safety Data Sheet
before performing this test.
HEALTH AND SAFETYHEALTH AND SAFETY
HEALTH AND SAFETYHEALTH AND SAFETY
HEALTH AND SAFETY
REFERENCESREFERENCES
REFERENCESREFERENCES
REFERENCES
Standard Methods for the Examination of Water and Waste-
water, 18th edition, 1992
10 mL
