Hanna Instruments HI 5421 User manual

HI 5421
DO/BOD/OUR/SOUR/Temperature
Bench Meter
Instruction Manual
www.hannainst.com
Printed in ROMANIA
Hanna Instruments Inc.
Highland Industrial Park
584 Park East Drive
Woonsocket, RI 02895 USA
Technical Support for Customers
Tel. [phone]
Fax [phone]
E-mail [email protected]
www.hannainst.com
Local Sales and Customer Service Office
MAN5421 03/15

TABLE OF CONTENTS
WARRANTY .............................................................................................................................................................. 2
PRELIMINARY EXAMINATION ..................................................................................................................................... 3
GENERAL DESCRIPTION ............................................................................................................................................. 3
FUNCTIONAL DESCRIPTION ....................................................................................................................................... 4
SPECIFICATIONS....................................................................................................................................................... 6
OPERATIONAL GUIDE................................................................................................................................................ 7
DISPLAYING MODES ................................................................................................................................................. 8
SYSTEM SETUP ...................................................................................................................................................... 11
DO SETUP ............................................................................................................................................................. 17
BOD SETUP ........................................................................................................................................................... 31
OUR SETUP ........................................................................................................................................................... 32
SOUR SETUP ......................................................................................................................................................... 33
DO CALIBRATION ................................................................................................................................................... 34
PRESSURE CALIBRATION......................................................................................................................................... 36
DO MEASUREMENT ................................................................................................................................................. 37
BOD MEASUREMENT ............................................................................................................................................... 41
OUR MEASUREMENT .............................................................................................................................................. 46
SOUR MEASUREMENT ............................................................................................................................................ 47
LOGGING ............................................................................................................................................................... 48
PC INTERFACE ........................................................................................................................................................ 52
PROBE CONDITIONING & MAINTENANCE .................................................................................................................. 53
TROUBLESHOOTING GUIDE ..................................................................................................................................... 54
ACCESSORIES ......................................................................................................................................................... 55
HI 5421 is warranted for two years against defects in workmanship and materials when used for their intended purpose
and maintained according to instructions. The probe is guaranteed for six months. This warranty is limited to repair or
replacement free of charge.
Damage due to accidents, misuse, tampering or lack of prescribed maintenance is not covered.
If service is required, contact the dealer from whom you purchased the instrument. If under warranty, report the model
number, date of purchase, serial number and the nature of the failure. If the repair is not covered by the warranty, you
will be notified of the charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain a Returned
Goods Authorization number from the Technical Service Department and then send it with shipping costs prepaid. When
shipping any instrument, make sure it is properly packed for complete protection.
To validate your warranty, fill out and return the enclosed warranty card within 14 days from the date of purchase.
Dear Customer,
Thank you for choosing a Hanna Instruments product. This manual will provide you with the necessary information for
correct use of the instrument.
Please read this instruction manual carefully before using the instrument.
If you need additional technical information, do not hesitate to e-mail us at [email protected] or view our worldwide
contact list at www.hannainst.com.
WARRANTY
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner.
RECOMMENDATIONS FOR USERS
Before using these products, make sure they are entirely suitable for the environment in which they are used.
Operation of these instruments in residential areas could cause unacceptable interferences to radio and TV
equipment, requiring the operator to follow all necessary steps to correct interferences.
The glass bulb at the end of the pH electrode is sensitive to electrostatic discharges. Avoid touching this glass
bulb at all times.
Any variation introduced by the user to the supplied equipment may degrade the instruments’ EMC
performance.
To avoid electrical shock, do not use these instruments when voltages at the measurement surface exceed
24 Vac or 60 Vdc.
To avoid damage or burns, do not perform any measurement in microwave ovens.
Hanna Instruments reserves the right to modify the design, construction or appearance of it’s products without
advance notice.
ACCESSORIES
HI 7040M Zero Oxygen Solution, 230 mL
HI 7040L Zero Oxygen Solution, 500 mL
HI 7041S Refilling Electrolyte Solution, 30 mL
OTHER ACCESSORIES
HI 710005/8 Voltage adapter from 115 Vac / 12 Vdc 800 mA (USA plug)
HI 710006/8 Voltage adapter from 230 Vac / 12 Vdc 800 mA (European plug)
HI 76404W Electrode holder
HI 76483 DO probe for laboratory use with built-in temperature sensor
HI 76483A/P 5 spare membranes
HI 92000 Windows®compatible software
HI 920013 USB cable

3
Remove the instrument from the packing material and examine it carefully to make sure that no damage has
occurred during shipping. If there is any damage, notify your dealer or the nearest Hanna Service Center.
The meter is supplied complete with:
•HI 76483 DO probe for laboratory use with built-in temperature sensor
•HI 7041S Electrolyte solution (30 mL)
•HI 76483A Membrane caps (2 pcs.)
•HI 76404W Electrode Holder
• 12Vdc Power Adapter
• Instruction Manual
HI 5421-01 is supplied with 12 Vdc/115 Vac adapter.
HI 5421-02 is supplied with 12 Vdc/230 Vac adapter.
Note: Save all packing material until you are sure that the instrument works properly. Any defective item
must be returned in the original packing with the supplied accessories.
HI 5421 is a professional bench meter with color graphic LCD for DO, BOD, OUR, SOUR and temperature
measurements.
The display viewing modes are: Basic information only, GLP information, Graph and Log History mode.
The main features of the instruments are:
• Single input channel;
• Capacitive touch keypad;
• Dedicated Help key with contextual message
• Six measurement parameters: DO, BOD, OUR, SOUR, pressure and temperature;
• Automatic or user standard DO calibration;
• AutoHold feature to freeze the stable reading on the LCD (DO only);
• Two selectable alarm limits (for DO, BOD, OUR, SOUR);
• Three selectable logging modes: Automatic, Manual or AutoHold (DO only);
• Up to 100 logging lots for automatic or manual modes, up to 200 OUR and SOUR reports and up to 200 BOD
method information entries;
• Selectable sampling period feature for automatic logging from 1 second to 180 minutes;
• GLP feature for DO;
• Online and offline graph;
• Large color backlight graphic LCD (240 x 320 pixels) with selectable color palette;
• PC interface via USB; download logged data to PC or use for Real time logging (HI 92000 PC application
required);
• Profile feature: store up to ten different user setups.
PRELIMINARY EXAMINATION
GENERAL DESCRIPTION
54
TROUBLESHOOTING GUIDE
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For an accurate calibration, it is recommended to wait a minimum of 15 minutes to ensure conditioning of the
probe. Keep the protective cap on during polarization and remove it for calibration and measurements. Follow the
calibration procedure.

4
HI 5421 DESCRIPTION
FRONT PANEL
REAR PANEL
1) Liquid Crystal Display (LCD)
2) Capacitive touch keypad
3) ON/OFF button
4) Power adapter socket
5) DO probe input
6) USB connector
FUNCTIONAL DESCRIPTION
53
PROBE CONDITIONING & MAINTENANCE
The DO probe body is made of PEI.
Use the protective cap provided when the probe is not in use. To inspect or replace the
membrane proceed as follows:
Inspection:
Inspect membrane surface to ensure it is in perfect condition. The semipermeable membrane
isolates the sensor elements from the environment but allows oxygen to enter. If any dirt is
observed on the membrane, rinse carefully with distilled or deionized water. If imperfections
still exist, or any damage is evident (such as salt crystals, wrinkles or tears-holes), the
membrane should be replaced. Verify no bubbles are trapped between the cathode and membrane.
Membrane Cap Installation:
For a new probe, remove the protective shipping tube by gently twisting and pulling it off the
body of the probe (see fig.1).
If a membrane cap was previously installed, unscrew the membrane cap by turning it clockwise
(see fig.2).
The new membrane cap should be rinsed with electrolyte solution
prior to use.
Fill the cap with clean electrolyte solution. Gently tap the sides of the
membrane cap to ensure that no air bubbles remain trapped. Do not
tap the bottom directly, as this will damage the membrane. Make
sure that the O-ring is seated properly inside the membrane cap (see
figure). With the sensor facing down, slowly screw the membrane cap
counterclockwise. Some electrolyte will overflow. When totally screwed
on, verify no bubbles are found trapped in the electrolyte.
Cathode Inspection:
The platinum cathode should always be bright and untarnished. Inspect
this when the cap is removed. If it is tarnished or stained, the cathode
should be cleaned. Use extreme caution when handling the probe tip
as the cathode insulator is glass. Inspect that the insulator has not
been cracked. Use a clean lint-free cardboard or cloth. Rub the cathode
very gently side to side 4-5 times. This will be enough to polish and
remove any stains without damaging the platinum tip. Afterwards, rinse
the probe with deionized or distilled water and install a new membrane
cap using fresh electrolyte and directions above.
Conditioning:
Before proceeding with the calibration make sure the probe is ready
for measurements. Reinstall the plastic protective cap over membrane
end. Reconnect probe to meter and allow probe to polarize.

5
KEYBOARD DESCRIPTION
FUNCTION KEYS
VIRTUAL KEYS
The upper row keys are assigned to the virtual keys placed on the bottom of the LCD, which allow you to
perform the displayed function, depending on the current menu (e.g. and
in Measure
mode).
LCD GENERAL DESCRIPTION
To enter / exit calibration mode.
To select the desired measurement mode: DO, BOD, OUR, SOUR.
To enter Setup (System Setup, DO Setup, BOD Setup , OUR Setup or SOUR Setup) and to
access Log Recall function.
To obtain general informations about the selected option / operation.
52
PC INTERFACE
Data transmission from the instrument to the PC can be done with the HI 92000 Windows®compatible
software (optional). HI 92000 also offers graphing and on-line help features.
Data logged on the HI 5421 meter can be exported to the most popular spreadsheet applications for further
analysis.
The instrument has an USB interface.
Use a standard USB cable to connect your instrument to the PC.
Make sure that the instrument and the HI 92000 software have the same baud rate and the appropriate
communication port.
The PC software may also be used for real time logging.

6
SPECIFICATIONS
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51
To delete lots:
• Press while in
Log Recall mode.
• Press or to access delete or delete all
mode. Otherwise, press to return to
Log Recall view
mode.
• After selecting one of the Delete keys, use or
to select one lot and then press or
to delete the selected lot or all lots. The “Please wait...”
message will be displayed on the LCD until the selected lot
or all lots are deleted.
• Press and then press to exit deleting mode
and return to Log Recall view mode.
• Press to exit
Log Recall mode and return to Measure mode.
Note: Logged lots should also be deleted whenever “Limited Automatic Logging Space” or “Automatic Log
Is Full” message appears on the LCD, in the Reminder messages area.

7
POWER CONNECTION
Plug the 12 Vdc adapter into the power supply socket.
Note: This instrument use non volatile memory to retain the meter settings, even when unplugged.
PROBE CONNECTION
For DO, BOD, OUR or SOUR measurements connect a DO probe to the DIN connector located on the rear panel
of the instrument.
INSTRUMENT START UP
• Please ensure that the capacitive keypad is not covered by hand or other objects at the meter power on.
• Turn the instrument on from the power button located on the rear panel of the instrument.
• Please wait until the instrument finishes the initialization process.
Note: It is normal for the loading process to take a few seconds. If the instrument doesn’t display the next
screen, restart the meter using the power button. If the problem persists, contact your dealer.
OPERATIONAL GUIDE
50
• Press , or to select the desired Log
Report type. All logged lots for the selected Log Report type
will be displayed on the LCD.
• To filter the displayed lots, press and then the
desired parameter. Only the selected measurement parameter
lots will be displayed on the LCD.
• Select the desired lot with or and press
to display the logged data from the highlighted lot.
The “Please wait...” message will be displayed on the LCD
for one second. The selected Logging Data Configuration
options will be displayed on the LCD, together with GLP
information (last calibration date and calibrated standards)
if a calibration has been performed on the selected mode
and the logged values (measured value, temperature value,
temperature compensation mode and the logging time).
Note: For automatic logging only, it is possible to view the
plotted graph.
• Press to display the graph.
• By pressing it is possible to move the graph along
the X or Y axis with the arrow keys.
• If pressing while the graph is displayed, the zoom
menu for the X and Y axes will be accessed. Press ,
or to switch between the active zooming axes and
then zoom in or out on the selected axis by pressing the
appropriate virtual key.
• Press to return to the previous menu at any time.

8
DISPLAYING MODES
For each measurement mode (DO, BOD, OUR or SOUR) the following display configurations are available:
Basic, Graph and Log History. GLP is available for DO measurements.
Basic
Accessing this option, the measured value and its units are
displayed on the LCD, along with the temperature value, temperature
compensation mode, pressure value, pressure compensation mode
and minimal GLP data.
To choose the Basic displaying mode:
• Press while in Measure mode. The “Choose
Display Configuration” message will be displayed in the
Reminder messages area.
• Press . The instrument will display the basic
information for the selected measurement mode.
GLP
Accessing this option for DO measurement, a detailed GLP data
will be displayed on the LCD: Last Calibration Date and Time,
Sample ID, Calibration Standards, Barometric Pressure, Salinity,
Temperature Values, the current Date and Time.
To access the GLP displaying mode:
• Press while in
Measure mode. The “Choose
Display Configuration” message will be displayed in the
Reminder messages area.
• Press . The instrument will display the detailed GLP
data.
49
3) Direct/AutoHold Reading Mode and Automatic Log
Press and then keys must be pressed on front
display to initiate this function. Real time continuous measurements
are on display with “AutoHold” flashing and real time continuous
logging into meter memory, until the meter reaches the stability
criteria to go into Auto Hold mode. The stored sample logs will
be marked with an “H” to indicate the Auto Hold mode. The virtual
key returns operation to real time continuous measurements
and stops the logging session.
4) Direct/AutoHold Reading Mode and Manual Log
Press in order to add one new record in the log report.
The manual log is working even if it is in Auto Hold or Continuous reading mode. Press to initiate
the Auto Hold event. “AutoHold” will flash until the stability criteria is reached and then the screen freezes
in Auto Hold mode, the data is marked with an “H”.
5) Direct/AutoHold Reading Mode and Auto Hold Log
Press and then keys to initiate and automate the capture of stable data which is stored in
the Recall Manual Log file. During the process, “AutoHold” will flash until the stability criteria is reached
and then the screen freezes in Auto Hold mode, the data is logged and marked with an “H”. The virtual key
returns operation to Real time continuous measurement. Press again to log a second stable
data point. The lot ID along with the record index will appear for short time on the top/left corner on the
selected channel window, every time a record will be added to the lot.
LOG RECALL
This feature allows the user to view all stored data. If no data
were logged, the “No records were found.” message will be
displayed on the LCD in the Log Recall screen. Otherwise, the
instrument will display all the memorized lots in accordance
with the selected option: Automatic Log, Manual Log or OUR/
SOUR Reports.
To view the memorized data:
• Press while in
Measure mode.
• Press and then select the log report type.

9
Graph
The online graph with real time logging (DO, BOD, OUR or SOUR
vs. Seconds) will be displayed when this option is selected.
If there is no active log, the previously logged data for the
selected parameter will be shown.
Notes: • If no data is being logged, the graph will be empty.
• If no automatic log is saved, the offline graph will be
empty.
To access the offline / online graph:
• Press while in
Measure mode. The “Choose Display
Configuration” message will be displayed in the Reminder
messages area.
• Press .
• Press to begin online graph.
To Zoom Graph
• Press then . and will appear in virtual keys.
•Use and to move along the X (Time) axis of the graph.
• Press to access the zoom menu for Y axis. Use or for zooming Y (parameter)
axis.
• Press to return to the main menu.
When the off-line graph is displayed:
• Use the arrow keys to move along the X (Time) and Y (parameter) axes of the graph.
• Press to access the zoom menu for X and Y axes. Use or to switch between the
active zooming axes. Press or to zoom the selected axis.
Note: While in zoom graph menu the key is not accessible.
• Press to return to the main menu.
48
LOGGING
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There are 5 ways the Reading Mode and Log may be configured together. The table below shows the
combinations and indicates where the completed log will be stored.
2) Direct Reading Mode and Manual Log:
Real time continuous measurements are on display and snapshots
of measurement data are stored in the Manual log when the user
presses . Subsequent snapshots will be added to the
same Manual Lot every time the is depressed unless
New Lot is selected under Log options.
Note: When the is pressed the lot ID along with the
current record number will appear for short time on the
selected channel window on the top/left corner (e.g.
L001_DO 14 - this means lot ID L001_DO and recod
number 14).
1) Direct Reading Mode and Automatic Log:
Real time continuous measurements are on display and continuous
logs to meter memory. These are sometimes referred as interval
logs. Press .

10
Log History
The measurement, along with LOG History, will be visible when
this option is selected:
1) The last stored logged data (Not actively logging) or
2) The last data logged from an active logging lot or
3) An empty display - NO LOTS saved, Not currently logging
the log history list also contains the main measured value, the
temperature, as well as a record time stamp.
To access the Log History displaying mode:
• Press while in
Measure mode. The “Choose
Display Configuration” message will be displayed in the
Reminder messages area.
• Press . The instrument will display the log history
regarding the selected Measure mode.
Notes: • When an alarm condition is active, the logged records will have an exclamation mark “!”.
• If logged in Auto Hold, logged records will have an “H”.
• If another Measure mode is selected, the Log History will reset.
• If the temperature unit is changed, all logged temperature values will be automatically displayed
in the new temperature unit.
•“A” denotes automatic temperature compensation.
•“M” denotes manual temperature compensation.
47
s
The Specific Oxygen Uptake Rate (SOUR), also known as the oxygen consumption or respiration rate, is defined
as the milligram of oxygen consumed per gram of volatile suspended solids (VSS) per hour. This quick measurement
has many advantages: rapid measure of influent organic load and biodegradability, indication of the presence of
toxic or inhibitory wastes, degree of stability and condition of a sample, and calculation of oxygen demand rates
at various points in the aeration basin.
The following equation is used for SOUR determination:
SOUR = OUR / Solids Weight
where:
OUR is the Oxygen Uptake Rate
Solids Weight is the
Total solids or the Volatile suspended solids weight in g/L
Temperature correction:
The SOUR value is corrected to 20 °C (68 °F) according to the Farrel and Bhide equation:
SOUR20 = SOURT x Θ (20 - T)
Where T is the measured temperature in °C and Θ is a temperature dependent variable:
Θ= 1.05 for T above 20 °C
Θ= 1.07 for T below 20 °C
This calculation is valid only for temperature values in the range 10 to 30 °C. Temperature correction is
performed only if the option SOUR @20°C is enabled (see
Method Configuration in SOUR Setup ).
Before starting a SOUR measurement remember to set the SOUR configuration from the setup menu and make
sure the has been calibrated in DO mode.
To measure the SOUR of a sample:
• Press and then to select SOUR measure
mode.
• Place calibrated probe into sample.
• Press to start taking the measurement.
• At the end of the measurement the meter will display the
computed SOUR value, the duration of the measurement and
the pressure and temperature values.
SOUR MEASUREMENT

11
SYSTEM SETUP
The System Setup menu allows the user to customize the user interface, view meter information, set the
external serial communication interface and to restore the manufacturer settings.
Accessing System Setup
• Press while in
Measure mode.
• Press . The system setup options will be displayed on
the LCD.
To access a System Setup option:
• Use or to highlight the desired option.
• Press to access the selected option.
The following is a detailed description of the System Setup
option screen.
Beeper
This option allows the user to turn an acoustic warning signal on
or off. This function can be used to signal 4 different events: a
stable signal, an alarm state, when every key is pressed or when
an incorect key is pressed. Enable (or disable) the Beeper for
these events. Disabling the Beeper will stop audible signals.
46
The OUR is used to determine the oxygen consumption or respiration rate. It is defined as the mg/L of oxygen
consumed per hour.
The following equation is used for OUR determination:
where:
DOSTART = Dissolved oxygen level at start of test
DOEND = Dissolved oxygen level at end of test
tELAPSED = Elapsed time of test in seconds
total volume/sample volume = Dilution factor of sample
Before starting an OUR measurement remember to set the OUR configuration from the OUR setup menu and make
sure the instrument has been calibrated in DO mode.
To measure the OUR of a sample:
• Press and then to select OUR measure mode.
• Place calibrated probe into sample.
• Press to start taking the measurement.
• At the end of the measurement the meter will display the
computed OUR value, the duration of the measurement and
the pressure and temperature values.
OUR MEASUREMENT

12
Saving Confirmation
Enable this option to force verification of a change made to a “GLP
Data Option field” or a Sample ID name. If Saving Confirmation is
enabled, the user will have to accept the change with a key stroke.
If Saving Confirmation is disabled, the changes made to these fields
change automatically without verification.
GLP Data
Use this option to customize log GLP information with specific identification data. When enabled, these ID tags
will be included in the GLP section of all data logs. Each data field can use up to 10 characters.
The five available fields are:
Operator ID : used to add the name of the operator
Instrument ID : used to name an instrument with a discrete name, location or number
Company Name : used to include the Company ID to the GLP data field.
Additional Info : Two data fields are available for general notes or notations.
To add the GLP Data:
• Press while in
Measure mode.
• Press .
• Use or to select the GLP Data option.
• Press and use or to highlight the
desired option.
• Press to edit the desired information. The Text Editor
menu will be displayed on the LCD.
• Enter the desired information by accepting the highlighted
character which is added to the text bar, using . The
and keys help the user select the desired character. It is also possible to delete the last
character by positioning the cursor on the Backspace character ( ) and pressing .
• Press to return to the GLP Data options. If the Saving Confirmation is enabled, press to
accept the modified option, to escape without saving or to return to the editing mode.
Otherwise, the modified options are saved automatically.
45
• If all samples/seeds were moved to the lower box, the
will appear.
• Otherwise, if some of the samples/seeds were selected, press
to change soft keys. will appear. Use
/ arrows to select sample, then press
.
• The BOD Results with SEED correction will be displayed.
Press to save and replace the previous sample report
with this bottle ID or press to keep the previous
sample BOD with no seed correction.
To print a copy of the BOD analysis with seed correction:
• Use HI 92000 software and connect the HI 5421 meter.
• Select the BOD sample from the available BOD reports.
• Press to print a paper copy of the analysis.

13
Date & Time
Set the current date & time and the format in which they appear. These parameters will be displayed on the
Measure screens and also when storing measured data.
Set Date and Time
This option allows you to set the current date (year/month/day) and time (hour/minute/second).
Notes: • Only years starting with 2000 are accepted.
• The time is set using the selected time format. For 12 Hour time format only, the AM/PM can
also be selected with or .
Set Time Format
Choose between 12-Hour (AM/PM) time format or 24-Hour time format.
Set Date Format
Choose the desired date format from 7 available options: DD/MM/YYYY; MM/DD/YYYY; YYYY/MM/DD; YYYY-MM-DD;
Mon DD,YYYY; DD-Mon-YYYY or YYYY-Mon-DD.
To set the Date & Time:
• Press while in
Measure mode.
• Press .
• Use or to select the Date & Time option.
• Press and use or to highlight the Set
Date and Time.
• Press to confirm your selection. Use /
to select next/previous entry to be edit. Press and use
or to set the desired value, then press
to save the modified value (for Set Date and Time option).
• For the other two options press to confirm your selection and select one of the displayed options.
• Press to return to previous menu. If the Saving Confirmation is enabled, press to accept the
modified option, to escape without saving or to return to the editing mode. Otherwise, the
modified option is saved automatically.
Note: If the time is changed with more than one hour before last calibration, a pop-up warning will appear
on the LCD, notifying the user that a date/time conflict has occured and some time-dependent modes
could work improperly (e.g.
Measure , GLP, Log).
44
SEED CORRECTION
To apply seed corrections to the sample data follow the following
procedure:
• Press then to select BOD mode.
• Press and to view samples.
Note: This list contains bottles with initial data, with 5 day BOD
determinations and 5 day BOD determinations with seed
corrections. The dates reflect the last date the sample was
evaluated.
• Press then to select the sample and seed
bottles.
• If more than one Seed Bottle is used, the average value of
the seeds will be used for Seed correction.
• Press to move samples into the lower box. These
bottles will be corrected with the selected seed values.

14
LCD Setup
This option allows the user to set the Contrast, the Backlight of the LCD and the Backlight Saver. The Contrast
parameter can be adjusted within 7 steps, while the Backlight parameter within 8 steps. The Backlight Saver can
be set from 1 to 60 minutes or it can be OFF (disabled). All the changes are visible on the LCD for each parameter.
Note: If the instrument backlight turnes off after the time period set, press any key to turn it back on.
To set the LCD Setup:
• Press while in
Measure mode.
• Press .
• Use or to select the LCD Setup option.
• Press and use key to highlight the desired
parameter.
• Use or to adjust the contrast / backlight or to
set the desired backlight saver time.
• Press to confirm the modified options and return to the
System Setup menu.
Color Palette
This option allows the user to choose a desired color palette.
To select the Color Palette:
• Press while in Measure mode.
• Press .
• Use or to select the Color Palette option.
• Press and use or to highlight the desired color.
• Press to confirm your selection and return to the System Setup menu or press
to return to the
System Setup menu without changing.
1roloCtxeteulbdnuorgkcabetihW
2roloCtxetetihwdnuorgkcabeulB
3roloCtxetkcalbdnuorgkcabetihW
4roloCtxetetihwdn
uorgkcabkcalB
43
• The Current dissolved oxygen measurement of the incubated
sample will be made.
• Press to apply dilution corrections and calculate BOD.
• The message ”Please wait until DO reading is stable” may
appear.
Note: If appears instead of , the incubation period
was less than 24 hours and calculation will not be possible.
After pressing the the message “Repeat Initial
Reading.” appears. If replacing Initial BOD is required then
press otherwise press .
• Remove probe from sample and rinse with purified water. Press
to analyze another prepared sample or seed.
The list will reflect the 5 day analysis.
Note: No Seed correction is made in these calculations at this step. This may be made manually by subtracting
the seed concentration from the samples, or made automatically with the meter (see SEED CORRECTION
section).
To print a copy of the BOD analysis of the sample:
• Use HI 92000 software to connect the HI 5421 meter.
• Select BOD sample fom available BOD samples.
• Press to have a paper copy of analysis.

15
Language
This option allows the user to choose the desired language in which all informations will be displayed.
To select the Language:
• Press while in
Measure mode.
• Press .
• Use or to select the Language option.
• Press and use or to highlight the
desired language.
• Press to confirm your selection and return to the System
Setup menu or press
to return to the System Setup
menu without changing.
Serial Communication
This option allows the user to set the desired speed for the serial communication (baud rate) in bps. The meter
and the PC program must have the same baud rate.
To set the Serial Communication:
• Press while in Measure mode.
• Press .
• Use or to select the Serial Communication
option.
• Press and use or to highlight the
desired baud rate.
• Press to confirm your selection and return to the System
Setup menu or press
to return to the System Setup
menu without changing.
Meter Information
This option provides general information about the instrument serial number (each instrument has an unique
identification serial number), the software version and the factory calibration date and time.
Note: All instruments are factory calibrated for DO, pressure and temperature. One year after factory calibration,
the warning message “Factory Calibration Expired” will be displayed when powering up the instrument.
The instrument will still function, however, it should be taken to the nearest Hanna Customer Service for
factory calibration.
42
• With the probe measuring the sample press . The initial
DO measurement will be saved.
• Remove probe from sample and fill and cover to prepare bottle
for incubation.
• Rinse probe off with purified water. If desired, move probe to
next sample. Press and repeat this procedure on
additional samples and Seed samples.
• At the conclusion of Day 0 the probe should be cleanned and
stored and all samples should be incubated following
Operational Procedures.
DAY 5 (Final DO)
• Remove samples and Seed samples from the incubator for
analysis.
• Press then select . Calibrate the DO probe.
• Press then select .
• Press .
• Press to access the list of the samples and seeds
available. The seed records will have the symbol “*” displayed
before the bottle ID.
• Select the first sample to analyze.
• Press . The Day 0 analysis of the sample will appear.
• Place the cleaned and calibrated DO probe into the sample
selected. The exact technique should follow standard operating
procedures.
• Press . The dissolved oxygen value of the sample
measured is displayed.

16
To view the Meter Information:
• Press while in
Measure mode.
• Press .
• Use or to select the Meter Information option.
• Press to acces the Meter Information menu.
• Press to return to the System Setup menu.
Restore Factory Settings
This option allows the user to erase all user settings and reset the instrument to the default factory settings.
To restore the Factory Settings:
• Press while in
Measure mode.
• Press .
• Use or to select the Restore Factory Settings
option.
• Press to confirm your selection. A pop-up menu will be
displayed, asking for confirmation.
• Press to confirm your selection and return to the System
Setup or press to return to the System Setup menu
without restoring defaults.
• Press
to return to Measure mode.
Software update
This function allows the user to update instrument software. In
order to start the PC upgrade application, you need to select the
proper baud rate, the software update package and start the
update.
41
Biochemical oxygen demand (BOD) is an indicator for the concentration of biodegradable organic matter
present in a sample of water. It can be used to determine the general quality of the water and its degree
of pollution. BOD measures the rate of oxygen uptake by microorganisms in a sample of water at a fixed
temperature and over a given period of time. To ensure that all other conditions are equal, a very small
amount of microorganism seed is added to each sample being tested. This seed is typically generated by
diluting activated sludge with deionized water. The samples are kept at 20 °C in the dark and tested for
dissolved oxygen (DO) after five days. The loss of dissolved oxygen in the sample, once corrections have
been made for the degree of dilution and seed addition, is called the BOD5.
Before running a BOD measurement remember to set the BOD method configuration from the BOD setup menu and
make sure the probe has been calibrated in DO mode.
Before starting the BOD procedure, calibrate the DO probe (see DO Calibration section).
• Press and then to select BOD measure mode.
• Press and then
.
• Use or to select Method Configuration option.
• Enter the operation limits for this method.
DAY 0 (Initial DO)
• Press and then followed by
to access the BOD management screen.
Note: The last sample analyzed will appear on the display.
• Press to add and measure a new sample or
to add and measure a new seed sample.
• Press then press .
• A field will appear to add ID, a description, bottle volume,
seed volume and sample volume.
• Place the DO probe in the sample bottle. Sample should be
well mixed. Follow all standard operating procedures.
• Press .
BOD MEASUREMENT

17
DO SETUP
The DO Setup menu allows the user to set the parameters related to the DO measurement and calibration.
Accessing DO Setup
• Press while in
Measure mode and then to
select the
DO measure mode.
• Press and then to access
DO Setup menu.
To access a DO setup options:
• Use or to highlight the desired option.
• Press to access the selected option or to exit
setup.
The following is a detailed description of the
DO Setup option
screens.
Profile
This option opens the Profile manager. Enabling Profile allows the user to Save, Load or Delete an
application Profile. The Profile option allows the user to store up to ten separate profile applications. Each
Profile can be named and recalled at a moment’s notice. A profile is a sensor setup complete with
measurement units, logging and display preferences, calibration standards, setup of the Display screen for
measurement (i.e. graphing, GLP) and any other sensor configuration. Once saved, the exact same profile can
be used at another time. This is a handy feature if the meter is used occasionally for additional applications
because it saves time in the setup of the meter and ensures the same procedure will be used.
To save the measurement configuration for DO mode:
• Press then and use or to highlight
Profile option.
• Press / to enable / disable this feature.
The available options are:
Profile Feature: enable or disable the profile feature.
Save Profile: save the current profile.
Save Profile As...: save current profile using a specific name.
Load Profile: load from available profiles.
Delete Profile: delete a profile.
40
The meter contains a built-in barometer, and it is able to automatically compensate for changes in barometric
pressure. If another pressure value than the barometer’s reading is to be used, then the manual pressure
feature must be enabled (See
DO Setup ).
The table below contains a conversion altitude (m) to pressure (mmHg) for the altitude values from the
previous table.
edutitlA
)m( 000300600900210051008100120042007200030033006300930004
erusserP
)gHmm( 067237507976456036706485365245225305484764164

18
Save Profile
To save a profile:
• Press while in
DO mode.
• Press .
• Use or to highlight
Profile option.
• Press
and then use or to highlight
Save Profile .
• Press . The existing configuration will be saved in current
profile.
Save Profile As...
To create a new profile:
• Press while in
DO mode.
• Press .
• Use or to highlight
Profile option.
• Press
and then use or to highlight Save Profile As... .
• Press . The
Text Editor box will be displayed on the LCD.
• Enter the desired profile name by using and to highlight the desired character and then
press to add it to the text bar. It is also possible to delete the last character by positioning the cursor
on the Backspace character ( ) and pressing .
• Press to return to the previous menu. If the
Saving Confirmation is enabled, press to accept
the modified option, to escape without saving or to return to the editing mode. Otherwise,
the modified option is saved automatically.
Note: The saved profile will automatically become the current
profile.
Load Profile
To load one profile:
• Press while in
DO mode.
• Press .
• Use or to highlight the
Profile option.
• Press
and then use or to highlight
the Load Profile option.
39
BAROMETRIC PRESSURE COMPENSATION
The dissolved oxygen saturation value varies with pressure, so it is important to compensate the effect that
pressure has on DO measurements.
C°
leveLaeSevobasreteM,edutitlA
F°
0
m
003
m
006
m
009
m
0021
m
0051
m
0081
m
0012
m
0042
m
0072
m
0003
m
0033
m
0063
m
0093
m
0004
m
06.411.416.311.316.211.217.112.118.014.010.017.93.90.99.80.23
28.313.318.214.219.115.110.116.012.019.95.92.98.85.84.86.53
41.316.212.217.113
.119.015.011.017.93.90.97.84.80.89.72.93
64.210.215.111.117.013.019.96.92.99.86.82.89.76.75.78.24
88.114.110.116.012.018.95.91.98.84.81.88.75.73
.72.74.64
013.119.015.011.017.94.90.97.84.81.88.75.72.79.68.60.05
218.014.010.016.93.99.86.83.80.87.74.71.79.66.65.66.35
413.019.96.92.99.85.82.89
.76.74.71.78.66.63.62.62.75
619.95.92.98.85.82.89.76.73.70.78.65.63.61.60.68.06
815.91.98.85.81.88.76.73.70.78.65.63.60.68.57.54.46
021.98.84.81.88.75
.73.70.77.65.62.60.68.56.55.50.86
227.84.81.88.75.72.70.77.65.62.60.68.56.54.53.56.17
424.81.88.75.72.70.77.65.62.60.68.56.54.52.51.52.57
523.80.87.74
.71.78.66.64.61.69.57.55.53.51.50.50.77
621.88.75.72.70.77.65.62.60.68.56.54.52.50.59.48.87
828.75.73.70.77.65.62.60.68.56.54.52.50.58.47.44.28
036.73
.70.78.65.63.60.68.56.54.52.50.58.46.46.40.68
233.70.78.65.63.61.68.56.54.52.50.58.47.45.44.46.98
431.78.66.63.61.69.56.54.52.50.59.47.45.43.43.42.39
638.66.63.61.69.57.55.53.51.59.47.45.44.42.41.48.69
836.64.61.69.57.55.53.51.59.47.45.44.42.41.40.44.001
044.62.69.57.55.53.51.59.47.46.44.42.41.49.39
.34.401
242.60.68.56.53.52.50.58.46.44.43.41.40.48.38.36.701
440.68.56.54.52.50.58.46.45.43.41.40.48.37.37.32.111
648.56.54.52.50.58.47.45.43.42.40.
49.37.36.35.38.411
847.55.53.51.59.47.45.44.42.40.49.37.36.35.34.34.811
055.53.51.59.47.46.44.42.41.49.38.36.35.34.33.30.221

19
Delete Profile
To delete one of the existing profiles:
• Use or to highlight the
Profile option.
• Press
and then use or to highlight
the
Delete Profile option.
• Press
. A list with all customised profiles will appear on
the screen.
• Use or to select the desired profile and press
.
• Press to return to previous menu.
Reading Mode
This option allows the user to select between Direct or Direct/AutoHold DO reading modes.
To set the reading mode:
• Press while in DO mode.
• Press .
• Use or to highlight the
Reading Mode option.
• Press or as desired.
• Press
to return to previous menu.
• Press
. A list with all customized profiles will be displayed on the screen.
• Use or to select the desired profile and press
to confirm or to exit without
selecting.
38
SALINITY COMPENSATION
If the sample contains significant concentration of salinity, the read out values must be corrected, taking into
account the lower degree of oxygen solubility in this situation.
Before taking any DO measurements remember to set the salinity value from the DO setup menu.
The salinity affects the DO concentration, decreasing its value. The table below shows the maximum oxygen solubility
at various temperatures and salinity levels.
C°
leveLaeSta)l/g(ytinilaS
F°
l/g0l/g01l/g02l/g03l/g53
006.4146.3147.2109.1105.110.23
218.3119.2170.2192.1119.015.63
490.3152.2174.1137.0183.012.93
644.2156.111
9.0122.0198.98.24
838.1190.1104.0157.944.94.64
0182.1185.0139.923.930.90.05
2177.0111.0105.929.856.86.35
4192.0186.901.955.803.82.75
6168.982.937.812.879.78.06
8154.909.893.809.766.74.46
0280.965.870.806.783.70.86
2237.832.877.733.721.76.17
4204.839.794.770.778.62.57
5
242.897.763.759.657.60.77
6290.856.732.738.646.68.87
8218.783.789.616.624.64.28
0345.741.757.693.622.60.68
2392.709.645.691.630.66.98
435
0.786.633.610.658.52.39
6328.674.641.638.586.58.69
8316.682.669.566.515.54.001
0414.690.697.505.563.50.401
2422.639.536.553.522.56.701
4440.677.584.512.590.52.111
6478.516.533.570.579.48.411
8407.574.502.559.458.44.811
0545.533.570.538.457.40.221
Note: The relationship between salinity and chlorinity for sea water is given by the equation below:
Salinity (g/l) = 1.80655 Chlorinity (g/l)

20
Temperature Unit
The user can choose from
Celsius , Fahrenheit or Kelvin temperature units.
To set the
Temperature Unit :
• Press while in
DO mode.
• Press .
• Press and then use or to highlight
the Temperature Unit option.
• Press and then use or to select
Celsius , Fahrenheit or Kelvin unit.
• Press to confirm your selection or press to
cancel operation.
Calibration
This selection allows configuration of options pertaining to calibration.
Standard Recognition
The user can choose between Automatic recognition (using 2 standards) or User Standard (a single point
calibration).
• Press while in
DO mode.
• Press .
• Use or to highlight the
Standard Recognition
option.
• Press or as desired
.
• Press to return to previous menu.
37
Make sure the instrument has been calibrated before taking DO measurements.
DIRECT MEASUREMENT
To measure the DO of a sample using the Direct reading mode:
• Press and then to select DO measure
mode.
• Select the Direct reading mode (see DO Setup ).
• Submerge the DO probe and allow time for the reading to
stabilize.
• The measured DO value will be displayed together with the
temperature and pressure values.
Notes: • For accurate DO measurements, a water movement of
0.3 m/s is required. This is to ensure that the
oxygen-depleted membrane surface is constantly
replenished. A moving stream will provide adequate circulation.
• If the reading is out of range, “-----” will be displayed.
DIRECT/AUTOHOLD MEASUREMENT
To measure DO of a sample using the Direct/AutoHold reading mode:
• Select the
Direct/AutoHold reading mode (see DO Setup ).
• If pressing , the “AutoHold” indicator will start
blinking on the display until the stability criterion is reached.
The DO value will be frozen on the display, along with
“AutoHold” indicator.
• To return to normal measure mode press .
DO MEASUREMENT