Hitachi Koki CS150NX User manual

HITACHI
TABLETOP
MICRO ULTRACENTRIFUGE
CS150NX
INSTRUCTION MANUAL
●The appearance or specification of the products covered in this manual is
subject to partial change for improvement.
－
Important
－
Before using this CENTRIFUGE carefully read
through this INSTRUCTION MANUAL to ensure
efficient, safe operation.
Keep this INSTRUCTION MANUAL available as an
important reference when using the CENTRIFUGE.
MFG.No. CAT.No. Manual code
S99806110
2014.11

Copyright © 2013 Hitachi Koki Co., Ltd.
All rights reserved. No part of this document may be reproduced or transmitted in any form or any means without
permission from Hitachi Koki Co., Ltd.
The names of actual companies and products mentioned herein may be the trademarks of their respective owners.

General description
The CS150NX is designed to separate liquid-suspended materials having different densities and
particle size.
The CS150NX is a product that pursues user-friendliness and reliability based on our many years
of experience in developing centrifuges. This centrifuge offers many features that we are confident
of satisfying your requirements. These features include the following.
1. The maximum speed is 150,000 rpm (1,050,000 x g).
2. Small and compact tabletop type
3. The touchscreen with easy-to-see color liquid crystal screen is incorporated.
4. The displayed language can be switched over between Japanese and English.
5. Various alarm indications can notify users of the causes and necessary actions of the troubles.
It can realize troubleshooting easier and quicker.
6. The rotor can be set by simply mounting it on the drive shaft in the rotor chamber (quick setting
type.)
7. Samples can be easily balanced visually (see Section 2-1-2).
8. This product spins very quietly, and is thus well suited for personal use.
9. A CFC-free thermomodule cooling system is employed featuring a powerful cooling capacity.
(Samples can be kept at 0℃at maximum speed when the ambient temperature is 30℃. )
10. The real-time control (RTC) feature enables setting a start time or a finish time, thus letting you
run your machine at a desired date and time.
11. Centrifugal force (RCFmax and RCFavg) can be displayed and set (Note 1).
12. Twenty varieties of nine stepped modes can be programmed for a wide range of applications
such as step running.
13. In addition to a door lock and an imbalance detector, two independent microprocessors are
incorporated for overspeed detection (a dual CPU overspeed prevention mechanism) for
even greater safety.
4
Note 1: RCF: Relative Centrifugal Force

i
SAFETY NOTICES
Safety reminders
Carefully read and fully understand the following safety instructions.
● Operate your instrument according to the instruction manual.
● Be sure to observe the all safety precautions in the instruction manual and safety
instructions on your instrument. If neglected, personal injury and/or instrument damage
can be caused.
● If the equipment is used in a manner not specified by the manufacturer, the protection
provided by the equipment may be impaired.
● The safety reminders are indicated as shown below. The signal words "DANGER",
"WARNING" and "CAUTION" are indicated together with the hazard alert symbols in this
manual.
"NOTE" indicates a note which has no direct bearing on personal safety.
Do not perform any operation not specified in the instruction manual. If any problem is
found on your instrument, contact a Hitachi Koki authorized sales/service representative.
Although the safety precautions in the instruction manual and safety instructions on your
instrument have been fully considered, an unexpected situation may arise. Observe the
instructions in the instruction manual and always be careful yourself when operating this
instrument.
DANGER：This note indicates an imminently hazardous situation, which if not
strictly observed, could result in personal severe injury or possible
death.
WARNING：This note indicates a potentially hazardous situation, which if not
strictly observed, could result in personal severe injury o
r
possible death.
CAUTION：This note indicates a potentially hazardous situation, which if not
strictly observed, could result in personal injury or severe damage
to the instrument.
This hazard alert symbol indicated together with a signal word is a
reminder to emphasize important safety instructions.

ii
SAFETY NOTICES
○Mechanical Safety
● Do not open the door while the rotor is spinning.
● Do not attempt to slow or stop the spinning rotor by hand.
● Do not incline or move the instrument while the rotor is spinning. Do not place
any object on the instrument or lean on the instrument.
● Do not attempt to unlock the door forcefully while the rotor is spinning.
● For operator safety, maintain a 30-cm "clearance envelope" around the
instrument while the rotor is spinning. Do not store dangerous substances
capable of developing flammable or explosive vapors on nor near the
centrifuge.
● Repairs, disassembly, and other modifications to the centrifuge are strictly
prohibited unless performed by a Hitachi Koki authorized sales/service
representative.
● Do not use the other’s manufacturer’s rotor without Hitachi Koki’s permission.
● Always use a quick-setting rotor for this ultracentrifuge. The screw-type rotors
are inapplicable.
● Check the chemical resistance chart attached to the rotor, and do not use any
sample inapplicable to the rotor (including the buckets). Using such a
sample could corrode the rotor (including the buckets).
● Do not exceed the maximum rated speed of the rotor or buckets in use.
● Do not use corroded, scratched or cracked rotor and buckets. Check that the
rotor and buckets are free of such abnormalities before operation.
● When using a swing rotor, check that the buckets are properly engaged with
the rotor pins before operation. Wrong setting can cause severe damage to
the instrument. Make sure that all the rotor buckets are of the same type.
● If abnormal sound or vibration occurs, stop the operation immediately and
contact a Hitachi Koki authorized sales/service representative.
● Position the centrifuge on a level surface, such a table or laboratory bench
that can support the weight of the centrifuge and resist vibration.
● Be sure to remove the rotor from the rotor chamber when centrifuge is not
used for a long time or when the machine is moved. Otherwise the drive shaft
(crown) may be damaged.
● Before using a rotor, be sure to read through the rotor instruction manual.
●
Check the chemical resistance chart attached to the rotor, and do not use any
sample inapplicable to the tubes, tube caps, bottles, or bottle caps, etc.
Using such a sample could corrode or deteriorate such parts.
●
Use the rotor tubes and bottles within their actual capacities.
●
Mount the rotor onto the drive shaft gently and properly.
●
Do not drop the rotor or apply excessive force to the drive shaft to avoid
damage to the drive shaft.
●
Maximum rotor speed depends on the tubes or adapters to be used. Follow
the instructions on the rotor instruction manual.
●
This centrifuge might convey vibration to a table while the rotor is rotating. If a
measuring device is positioned on a table and near this centrifuge, use a
measuring device carefully.
●
Approximately even quantities of sample in the tubes are sufficient for
balancing, and extremely different sample quantities must be avoided ( Refer
to Section 2-1-2 for the sample balancing).
●
Clean the inside of the drive hole (crown hole) of the rotor and the surface of
the drive shaft (crown) of the centrifuge once a month.
●
Storing the rotor on the shelf is permitted if the shelf is taken necessary
countermeasures against earthquakes not to drop the rotor.
WARNING：
CAUTION：

iii
SAFETY NOTICES
● Do not pour any solution such as water, detergent, or disinfectant directly into
the rotor chamber and be careful not to spill the sample into the rotor chamber.
If you do so, the bearings of the drive unit might corrode or deteriorate and it
might cause vacuum failure.
● Do not operate the display panel (Touchscreen) using a ball-point pen.
○Safety during installation and maintenance
● Before removing the cover, top deck, or other component for maintenance,
always turn off the POWER switch of the instrument, unplug the power cord
from the wall outlet, and wait at least three minutes to avoid the risk of
electrical shock.
● For maintenance and repairing of the rotors, tubes, etc., see the rotors and
tubes instruction manual.
● After installation and before any test-run, this ultracentrifuge always needs the
internal check by a Hitachi Koki authorized sales/service representative.
● Repairs, disassembly, and other modifications to the centrifuge are strictly
prohibited unless performed by a Hitachi Koki authorized sales/service
representative.
● If the centrifuge is exposed to ultraviolet rays for a long time, the color of the
covers may be changed or the coating may be peeled off. After use, cover
the centrifuge with a cloth to protect it from direct exposure.
○ Electrical Safety
●
Your centrifuge must be grounded properly to avoid electrical shock hazards.
●
Do not place containers holding liquid in the rotor chamber or on or near the
instrument. If they spill, liquid may get into the instrument and damage
electrical components.
●
If the machine will not be used for a long time, turn off the main circuit breaker.
WARNING：
CAUTION：
DANGER：
CAUTION：
CAUTION：
WARNING：

iv
SAFETY NOTICES
○Safety against Risk of Fire
● This centrifuge is not explosion-proof. Never use explosive or flammable
samples or materials that chemically react vigorously. Do not centrifuge such
materials in this instrument nor handle or store them near the instrument.
○Chemical and Biological Safety
● Take all necessary safety measures before using samples that are toxic or
radioactive, or blood samples that are pathogenic or infectious. You use such
samples at your own responsibility.
●
Take all necessary safety measures when Risk Group Ⅱmaterials (as
identified in the World Health Organization “Laboratory Biosafety Manual”) are
handled, and that more than one level of protection shall be provided in the
case of materials of a higher group.
●
If the centrifuge, rotor, or an accessory is contaminated by samples that are
toxic or radioactive, or blood samples that are pathogenic or infectious, be
sure to decontaminate the item according to good laboratory procedures and
methods.
● If there is a possibility that the centrifuge, rotor, or an accessory is
contaminated by samples that might impair human health (for example,
samples that are toxic or radioactive, or blood samples that are pathogenic or
infectious), it is your responsibility to sterilize or decontaminate the centrifuge,
rotor, or the accessory properly before requesting repairs from a Hitachi Koki
authorized sales/service representative.
● It is your responsibility to sterilize and/or decontaminate the centrifuge, rotor,
or parts properly before returning them to a Hitachi Koki authorized
sales/service representative.
Notice for an Earthquake
Depending on the magnitude, an earthquake might damage centrifuge. If you observe some
abnormality, stop using the centrifuge immediately and ask for inspection by the Hitachi Koki
service representative.
WARNING：
WARNING：

v
Table of contents
1. Description ････････････････････････････････････････････････････････････････1-1
1-1 External view of ultracentrifuge ･･･････････････････････････････････････････ 1-1
1-2 Structure ･･････････････････････････････････････････････････････････････ 1-2
1-2-1 Touchscreen ･･････････････････････････････････････････････････････ 1-2
1-2-2 Rotor chamber･････････････････････････････････････････････････････ 1-5
1-2-3 Safety devices ･････････････････････････････････････････････････････ 1-6
2. Operation ･････････････････････････････････････････････････････････････････ 2-1
2-1 Run preparation ････････････････････････････････････････････････････････ 2-2
2-1-1 Starting up this machine ･･･････････････････････････････････････････ 2-2
2-1-2 Preparing tubes/bottles and rotor ････････････････････････････････････ 2-3
2-1-3 Applicable rotors ･･････････････････････････････････････････････････ 2-4
2-2 Basic operation･････････････････････････････････････････････････････････ 2-5
2-2-1 Setting run conditions ･･･････････････････････････････････････････････ 2-5
2-2-2 Operational procedure ･･････････････････････････････････････････････ 2-10
2-2-3 Acceleration rates and deceleration rates･･･････････････････････････････ 2-13
2-3 How to use the option function････････････････････････････････････････････ 2-14
2-3-1 Programmed operation･･････････････････････････････････････････････ 2-15
2-3-2 Step-mode operation ･･･････････････････････････････････････････････ 2-30
2-3-3 Selecting the rotor･･････････････････････････････････････････････････ 2-41
2-3-4 Displaying and setting RCF ･･････････････････････････････････････････ 2-43
2-3-5 User login･････････････････････････････････････････････････････････ 2-47
2-4 Features of the MENU screen ･････････････････････････････････････････････ 2-49
2-4-1 Displaying the Run History and loading the information about run parameters ･･････ 2-50
2-4-2 Rotor Catalog ･････････････････････････････････････････････････････ 2-51
2-4-3 Spin-down operation････････････････････････････････････････････････ 2-52
2-4-4 RTC (real-time control) operation ･････････････････････････････････････ 2-53
2-4-5 Customizing the settings ････････････････････････････････････････････ 2-57
2-4-6 Administrator (Admin) functions･･･････････････････････････････････････ 2-60
2-5 Emergency recovery from power failure････････････････････････････････････ 2-73
3. Maintenance ･･････････････････････････････････････････････････････････････ 3-1
3-1 Rotor Chamber ･････････････････････････････････････････････････････････ 3-2
3-2 Drive Shaft (Crown) ･････････････････････････････････････････････････････ 3-2
3-3 Cabinet････････････････････････････････････････････････････････････････3-2
3-4 Chamber door seal ･････････････････････････････････････････････････････ 3-3
3-4-1 Removing the door seal O-ring ･･･････････････････････････････････････ 3-3
3-5 Vacuum pump･･････････････････････････････････････････････････････････ 3-4
3-6 Others ････････････････････････････････････････････････････････････････3-4

vi
Table of contents
4. Troubleshooting ··········································································································4-1
4-1 Alarm indicators ······································································································4-2
4-2 User-corrected Problems························································································4-4
5. Installation ····················································································································5-1
6. Warranty ·······················································································································6-1
7. Specifications ··············································································································7-1
8. Supply list ·····················································································································8-1
APPENDIX
Decontamination sheet
WEEE Compliance
Marking for Control of Pollution Caused by Electronic Information Products
(THE PEOPLE’S REPUBLIC OF CHINA)