I-Scope DN117D-LED User manual

DN117D-LED
I-SCOPE OPTICS JAPAN
Digital Microscope
Model Number
DN117D-LED
User Manual

DN117D-LED
I-SCOPE OPTICS JAPAN
1
CONTENT
User Notice............................................................................................2
1. Components.......................................................................................4
2.Assembly ............................................................................................5
2.1 Assembly Diagram.....................................................................5
2.2 Assembly Steps...........................................................................6
3.Adjustment & Operation ..................................................................10
3.1 Adjustment Sets........................................................................10
3.2 Operation..................................................................................11
4. Specification Table ..........................................................................14
4.1 Main specifications ..................................................................14
4.2 Eyepieces and Objectives.........................................................14
4.3 Total Magnification..................................................................15
5．Outfit..............................................................................................15
6．Troubleshooting Guide..................................................................16

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User Notice
Ⅰ. Safety Notes
1. Carefully open the box, avoid the accessories, like lens, dropping to ground and being damaged.
2. Do keep the instrument out of direct sunlight, high temperature or humidity, dustyand easy
shaking environment. Make sure the stage is smooth, horizontal and firm enough.
3. When moving the instrument, please use two hands to grip with the two sides of the microscope
body.
4. When running, the lamp house and nearby parts will be very hot. Please ensure there is enough
cooling room for them.
5. Make sure the instrument is earthed, to avoid lighting strike.
6. For safety, be sure the main switch is in “O”(off) state before replacing the halogen lamp or the
fuse, then cut off the power, and do the operation after the lamp bulb and the lamp house
completely cool down.( Specified: LED 3W)
7. Check the input voltage: be sure the input voltage which signed in the back of the microscope is
consistent with the power supply voltage, or it will bring a serious damage to the instrument.
8. Use the factory supplied power cord, please.
II. Maintenance
1. All the lenses have been well checked and adjusted. It is forbidden to disassemble them yourself.
2. The nosepiece and coarse/fine focus unit have a compact and precise frame; please don’t
disassemble them as possibly as you can.
3. Keep the instrument clean, wipe dust regularly, and be attention to avoid contaminating the
optical elements especially.
4. The contaminations on the prism, as finger mark and oil, could be gently wiped with a piece of
soft cloth or tissue paper, gauze which has been immersed in pure alcohol or ether. (Note that
the alcohol and ether are highly flammable, do keep them away from the fire or potential
sources of electrical sparks, and use them in a drafty room as possible as you can.)
5. Do not attempt to use organic solvents to clean the microscope components other than the glass
components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted neutral
detergent.
6. When using, if the microscope is splashed by liquid, cut off the power at once, and wipe up the
moisture.
7. Do not disassemble any parts of the microscope, which will affect the function or decline the

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performance of the microscope.
8. Place the instrument in a cool, dry position. When not using the microscope, keep it covered
with a dust cover. Make sure the lamp socket is cool before covering the microscope.

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1. Components
Digital Biological Microscope
Eyepieces
Digital viewing head
双目观察头
Nosepiece
Objectives
Main body
Condenser
Collector

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2.Assembly
2.1 Assembly Diagram
The following figure shows the installation sequence of the components. The number in the figure
shows the assembly steps.
Before installing, be sure every components is clean, do not score any parts or glass surface.
Keep well with hexagon wrench provided. When replacing the components, you will need it
again.
③
Main body
Objectives
Digital viewing head
Eyepieces
④
①
②
Power cord
USB data wire
⑤

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2.2 Assembly Steps
2.2.1 Installing digital viewing head
（Fig.1、2）
Insert the digital viewing head into the
microscope head, turn into the right position,
then screw down the bolt①to fix it.
2.2.2 Installing the eyepieces（Fig.3、
Fig.4）
Insert the eyepieces into the eyepiece tube
until they are against each other as shown in
Fig.4.
NOTE:
Operation Conditions：
1．Temperature：0℃～40℃,Maximum
Relative Humidity：85%.
2．High Temperature：High Temperature
and humidity will result in a mildewing,
dew and even ruinous instrument.
3．Avoid placing the instrument in a dusty
environment. When ending your
microscope operation, please cover it with
the dust cap.
4．Lay the microscope in a plan and stable
position, please.
Fig.1
Fig.3
①
Fig.2
Fig.4

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②
①
Fig.6
Fig.5
2.2.3 Installing objectives (Fig.5& 6）
1. Adjusting the coarse focus knob until the
support device of the mechanical stage
reaches its low limit position.
2. Screw the lowest magnification objective
into the nosepiece from the left or the right
side, then revolve the nosepiece clockwise
and mount other objectives by the sequence
of low to high magnification
Installing objective this way will make the
change of magnification to be easier during
using.
Clean the objectives regularly, for lens is
susceptible to dust.
When operating, use 10×magnification
objective to search and focus specimen
firstly, then replace with higher
magnification objective if necessary.
When replacing the objective, slowly turn
the nosepiece until you hear “clicked”,
which means the objective is in the
required position—center of the light
path.
2.2.4 Installing the color filters (Fig.7)
1. Turn the condenser bracket①out at the
direction of arrow in Fig.7
2．Put the required filters②into the holder on
the bracket, and then turn the bracket back
to the right position.
★Baby blue and green filters are available in
standard outfit.
Fig.7

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③②
⑥
④⑤
2.2.5 Connecting the power cord
(Fig.8, 9,10)
★The cable and cords are vulnerable when
bent or twisted, never subject the power
cord to excessive force.
1. Turn the main switch①to “O” (off) state
before connecting the power cord.
2. Insert the power plugs②into the power jack③
of the microscope; make sure the connection is
well.
3. Plug the power cord ④into the power supply
receptacle safely. Make sure the connection is
well.
★Do use the supplied power cord all the
time. If it lost or damaged, select the same
standard cord, please.
★Either 110V or 220V can be selected as
the input voltage of this microscope. (The
input voltage has been preset in the
microscope before leaving factory.
2.2.6 Replacing the Fuse（Fig.8、9、10）
Do remember to set the main switch①to
the state of “O” (OFF) and unplug the power
cord ②before replacing the fuse. Scratch the
fuse holder⑤out from the power socket③
on the microscope, replace with a new fuse in
the holder, then press the fuse holder back
again.
★There is a spare fuse in the fuse holder.
★For 220V input voltage，use fuse of rating
(250V500mA).
★For 110V input voltage, use fuse of rating
(250V1A).
Fig.8
①
Fig.9
Fig.10

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②
①
④
.
⑥
⑤
2.2.7 Installing and replacing the lamp
（Fig.11,12,13）
There are two types of illuminator available
for this microscope, one type is halogen lamp
6V20W, the other is 3W LED. During use or
just after using, the lamp house and nearby parts
will be very hot. Please set the main switch to
“O” (off) state before replacing, and make sure
the bulb, the lamp room and periphery are all cool
enough to carry no burn. Then, you can do your
replacing.
1. Loose the bolt①and open the window②on
the bottom of the microscope base with
“一”type screwdriver.
2. Pull out the old bulb③,hold the new bulb
after you wrap it with gauze or other
protection materials and insert its pin as
deeply as possible into the jack in the lamp
holder.
4. Close the window and tighten the bolt①.
Please insert the bulb gently, or it will
be damaged by excessive extrusion.
Do not touch the halogen bulb with
bare hands. It will shorten the service life
or cause it to burst. If you leave
fingerprints on the surface carelessly,
clean it with a piece of dry soft cloth.
When replacing LED ：
1. Generally, the LED has long service life and
is not easy to damage, if unfortunately it
damaged, purchase a new one from the
supplier.
2. Remove the base plate⑥from the bottom
side of the microscope base with screw
driver ,loose the bolt⑤to take the old LED
off, replace with a new one .
3. Put the new LED unit back onto the bracket
with the bolt⑤and the base plate⑥onto the
bottom side of the microscope.
When you take down the base plate,
please do it gently and slowly, to avoid
damaging internal electrical wires.
Fig.11
③
Fig.13
Fig.12

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3．Adjustment & Operation
3.1 Adjustment Sets（Fig.14,Fig.15）
Main switch
Brightness Adjustment Knob
Fig.15
Tension Adjustment Collar
Right Coarse Focus Knob
Right Fine Focus Knob
Left Fine Focus Knob
Left Coarse Focus Knob
CondenserAdjustment Knob
Aperture DiaphragmAdjustment Lever
Longitudinal Adjustment Knob
Transversal Adjustment Knob
Diopter Ring
Interpupillar Distance Indicator
Fig.14

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3.2 Operation
Spanner
Fig.19
Fig.17
③
④
①
②
Fig.16
Fig.18
①
③
②
3.2.1Adjusting the brightness（Fig.16）
1. Connect the power, turn on the main
switch①(shown in the figure)which on the
bottom side of the base to “－”(on).
2. Turning the brightness adjustment knob ②
clockwise, the voltage decline, and the
brightness weaken; Whereas turning at the
opposite direction, the voltage raise, and the
brightness strengthen.
Using the microscope at a lower voltage
can prolong the service life of the bulb.
3.2.2 Placing the specimen（Fig.17）
1. Place the specimen③on the center of the stage,
and then hold it with the specimen holder④.
2. Turn the transversal and longitudinal adjustment
knobs which on the mechanical ruler to move the
specimen onto the required position.
★Be careful when changing the objective. If
you finish the observation with the short
working distance objective, and want to
change another one, be careful of not letting
the objective touch the specimen.
3.3.3 Focusing the specimen(Fig.18、19）
1. Focus the specimen with 10X objective. To avoid
the objective touching the specimen during
focusing, you should raise the mechanical stage to
let the specimen close to the objective at first, then
slowly dispart them to bring the specimen to focus.
2. Turn the coarse focus knob ①conversely to lower
the specimen and search images in the 10×ocular
simultaneously, and then use the fine knob②to
make focus. After that, you can replace with other
magnification objectives safely, and focus without
the risk of damaging the specimen.
●The tight tension of the coarse focus knob has
already been adjusted before leaving factory.
If loosen (e.g. the stage slip down by its
weight), please screw the intention adjustment
collar③to the right position by the supplied
spanner.

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3.3.4 CondenserAdjustment（Fig.20）
Turn the condenser focus knob to move
the condenser up and down. Raise the
condenser when using the high
magnification objective, and descend it
when using the low magnification one.
The condenser and the objective are
coaxial. It has been adjusted well before
leaving factory, so the user needn’t to
adjust them by self (the distance between
the top of the condenser and the stage
should be in the range of
0.03mm~0.4mm.)
The highest position of the condenser has
been adjusted too. It also needn’t any
user’s operation.
3.3.5 Aperture Iris Diaphragm
Adjustment（Fig.20,21）
Turn the aperture iris diaphragm lever①
to adjust the aperture iris diaphragm.
●Generally, setting the aperture iris
diaphragm to 70- 80% of the N.A. of the
objective in use will provide an image
with good contrast.
●If the size of the aperture diaphragm
minified, the brightness and the
resolution declined, while the contrast
and the depth of field increased; In
other words, if the size largen, the
brightness and the resolution improved,
but the contrast and the depth of field
declined.
●Generally, setting the size of the
condenser aperture diaphragm at
70%～80% of the numerical aperture,
you can obtain a clear image with
enough contrast. If the open of the
aperture diaphragm is too small, the
resolution were very low, so please don’t
minify the aperture below 60% of the
objective’s numerical aperture unless in
a special case, for instance, observating
an almost transparent specimen.
①
Fig.20
Magnification：10×
N.A.：0.25
Fig.21
Specimen
Image ofAperture Iris Diaphragm

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●The numerical aperture is marked on the objective. For example, the mark “10/0.25 ” means the
magnification is 10×，and the numerical aperture is 0.25.
●If you want to observe the image of the aperture iris diaphragm, remove one eyepiece and look
through the tube. You will see a dark circle encroaching on the bottom of the tube.
3.3.6 Adjusting the Interpupillary
Distance（Fig.22）
The interpupillary distance range:
48mm～75mm。
When observing with two eyes, hold on the
left and right prism holders, turn them around
the axis to adjust the interpupillary distance
until the left and right fields of view coincide
completely, as shown in Fig.22.
3.3.7 Adjusting the diopter（Fig.23）
1. Looking through the right ocular with your right
eye, revolve the coarse and fine focusing
adjustment knob to focus on the specimen.
2. Then look through the left ocular with your left
eye. If the image is not sharp, turn only the left
diopter adjustment ring①to focus on the
specimen please.
★The diopter range of the eyepiece is ±5
diopter. The number aligned to the line on the
viewing head is the diopter in use.
Fig.22
Fig.23
①

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4. Specification Table
4.1 Main specifications
Mechanical Tube
Length
160mm
Viewing Head
Compensation free binocular head, , Inclined at 30°,Interpupilary Distance 48-75
mm
Eyepiece
Field of view: φ18mm
Nosepiece
Backward Quadruple Nosepiece
Objective
Achromatic objectives 4×，10×，40×、100×
Focusing
Coaxial coarse and fine focusing knob; the minimum division of fine
focusing:0.004mm; focusing adjustment range: 24mm
Condenser
Abbe Condenser，NA=1.2 with iris diaphragm
Stage
Double Layers Mechanical Stage 132mm×142mm，Moving Range 74×40mm
Illumination
Halogen Lamp 6V20W or LED 3W
4.2 Eyepieces and Objectives
1. Objectives
Magnification
Numerical
Aperture
（NA）
Thickness of
glass slide
(mm)
Focal length
（mm）
Working
Distance（mm）
Type
4×
0.10
0.17
31.05
18
Dry
10×
0.25
0.17
17.13
6.5
Dry
40×
0.65
0.17
4.65
0.53
Dry
100×
1.25
0.17
2.906
0.13
Oil
2. Eyepieces
Category
Magnification
Focal length
f（mm）
Field of view（mm）
Plan eyepiece
10×
24.95
Φ18

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4.3 Total Magnification
Eyepiece
10×
10×
10×
10×
Objective
4×
10×
40×
100×
Total
Magnification
40×
100×
400×
1000×
5．Outfit
Component Name
Specification
Quantity
Standard
Outfit
Main body
Main Standard
1
〇
Double Layers Mechanical Stage
1
〇
Condenser Holder
1
〇
Viewing Head
Compensation free digital binocular head
1
〇
USB data wire(2 meters)
1
〇
Condenser
Abbe condenser for bright field with iris
diaphragm
NA=1.2
1
〇
Nosepiece
Quadruple
1
〇
Illumination
Halogen Lamp 6V20W (or LED 3W)
1
〇
Spare lamp （6V20W Halogen lamp ）
2
〇
Spare fuse ( 50T250V2A or 500mA)
1
〇
Eyepieces
10×Plan Eyepieces
2
〇
Objectives
Achromatic objective 4×
1
〇
Achromatic objective 10×
1
〇
Achromatic objective 40×
1
〇
Achromatic objective 100×（oil、spring）
1
〇
Filter
Baby Blue, Green
1 ea.
〇

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6．Troubleshooting Guide
1. Optical system
TROUBLE
CAUSE
SOLUTION
The edge of the
field of view is dark
or the brightness is
not uniform
The nosepiece is not in the located position (objective and
light path not coaxial)
Locate the nosepiece
properly where it clicks
The image of filament is not centered
Center the filament
A lens (the objective, condenser, eyepiece or collector) is
dirty.
Clean it thoroughly
Find dust and stain
in the field of view
There are stains on the lens（including condenser,
objective, eyepiece and collector）
Clean it up
There are stains on the specimen
Clean it up
The position of the condenser is too low
Loosen the condenser’s
locking bolt, adjust the
condenser to the right
position
The image is
defocused ( low
resolution \
contrast)
There is no cover slip on the specimen
Add coverslip
The cover slip is too thick or too thin
Use the standard
coverslip ( 0.17mm )
The specimen is placed inversely
Reversal it back
There was oil on the dry objective(easily happened in
40X objective)
Clean it up
There are stains on the lens（including condenser,
objective, eyepiece and collector）
Clean it up
didn’t use oil for the oil objective
Use immerse oil
There was bleb in the oil
Eliminate the bleb
Use a unsuitable oil
Change to the specified
one
The size of the aperture diaphragm is too big
Minify it
There are stains on the incident lens of the binocular tube
Clean it up
The size of the aperture diaphragm is too small
Open it up
The position of the condenser is too low
Adjust the position
One side of the
image is dark
The condenser is not in the center of the field of view\the
condenser inclines
Install the condenser
again and adjust the
center carefully by
centering the bolt
The nosepiece is not in the right position
Turning it until it reach
the “clicked” position
The specimen is floating
Fix it
The image shift
during focusing
The specimen slips on the stage
Fix it
The nosepiece is not in the right position
Turn it to the
“ clicked ”position

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The image is a little
yellow
Not use the blue color filter
Use the blue filter
The brightness is
not enough
The size of the aperture diaphragm is too small
Adjust again
The position of the condenser is too low
Adjust the position
There are stains on the lens（including condenser,
objective, eyepiece and collector）
Clean it up
2. Mechanical system
TROUBLE
CAUSE
SOLUTION
The image can not
focus when using high
magnification
objective
The specimen is placed inversely
The coverslip is too thick
Turn inversely
Use the standard coverslip（0.17
mm）
The objective touch
the specimen when
changed from low
magnification to the
higher magnification
The specimen is placed inversely
The coverslip is too thick
Turn inversely
Use the standard coverslip（0.17
mm）
The specimen is not
easy to move
The specimen holder is not fixed
Fix it
The binocular image is
not coincident
The interpupillar distance is not correct
Adjust it
Eyes are too tired
No diopter adjustment
Adjust the diopter correctly
The brightness is not suitable
Adjust the voltage of the lamp
3. Electrical system
TROUBLE
CAUSE
SOLUTION
The lamp can’t light
when the switch is
turned on
No power
Check the connection of the
power cord
The bulb is not inserted
Insert it correctly
The bulb burns out
Replace it
The lamp burns out
suddenly
Use a substandard lamp
The voltage is too high
Use the specified lamp to replace,
if the problem is not solved,
contact with the service department
The brightness is not
enough
Use a substandard lamp
The voltage is too low
Use the specified lamp
increase the voltage
The bulb flickers or
the brightness is
vertiginous
The bulb is going to burn out
Replace it
The bulb is not entirely inserted into the
holder
Check and insert it again

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