Implen NanoPhotometer N120 User manual

NanoPhotometer®N120/NP80/N60/N50/C40
User Manual
Version 4.3.1
Software Version 4.3.15670

NanoPhotometer®N120/NP80/N60/N50/C40 User Manual Version 4.3.1
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The end user of the NanoPhotometer®product (“End User”) hereby takes full responsibility
for safe storage and backup of all files and/or data that may be created, saved on or
transferred from the device. End User acknowledges that it is possible that data and/or files
may be lost or damaged, and further acknowledges and agrees that it has sole responsibility
to maintain all appropriate backup of files and data. By using the NanoPhotometer®device,
End User hereby agrees to these terms, and agrees that Implen shall not be held liable for
any loss, deletion or damage of any data or files for any reason, including any damages
attributable thereto.
Telephone support is available using one of the following phone numbers from your
geographic region:
Europe, Asia, South Pacific, North and South America
Middle East, Africa
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]
Email: [e-mail] Email: [e-mail]
Website: www.implen.de Website: www.implen.com
Implen GmbH Implen, Inc.
Schatzbogen 52 Unit 104
81829 München 31194 La Baya Drive
Germany Westlake Village, CA 91362
USA
Windows and Excel are trademarks of Microsoft Corporation Redmond, WA
macOS is a trademark of Apple Inc. Cupertino, CA

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Declaration of conformity for the NanoPhotometer®
(C40/N50/N60/NP80/N120)
This is to certify that the Implen NanoPhotometer®conforms to the requirements of
the following directives:
2014/35/EU Low Voltage Equipment Safety Directive
2014/30/EU Electromagnetic compatibility (EMC) directive
IEC 60529 Protection class IP20
2011/65/EU Restrictions on the use of certain Hazardous Substances
in Electrical and Electronic Equipment (ROHS)
2012/19/EU EC Directive on Waste Electrical and Electronic Equipment
(WEEE) 2003/108/EC & 2008/34/EC. By ensuring this product is
disposed of correctly, you will help prevent potential negative
consequences for the environment and human health, which
could otherwise be caused by inappropriate waste handling of
this product.
FCC 47 CFR Part15 §15.107 and §15.109
EN 301 489-1 V1.9.2 Radio and ancillary equipment for portable use (portable
equipment); EUT Operating frequency range: 2.4 –2.4835 GHz
EN 300 328 V1.8.1 Electromagnetic compatibility and Radio spectrum Matters
(ERM); Wideband transmission systems; Data transmission
equipment operating in the 2,4 GHz ISM band and using wide
band modulation techniques; Harmonized EN covering the
essential requirements of article 3.2 of the R&TTE Directive
EN 301 489-17 V2.2.1 Electromagnetic compatibility and Radio Spectrum Matters
(ERM)
IEC 62133 and UN38.3 Battery certification and transport test
Standards to which conformity is declared, where relevant, are as follows:
IEC/EN 61010-1:2012 Safety requirements for electrical equipment for measurement,
control, and laboratory use. General requirements.
EN61326-1:2013 Electromagnetic compatibility- generic emission standard
electrical equipment for measurement, control, and laboratory
use.
Signed:
Dr. Thomas Sahiri
Managing Director
Implen GmbH

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Contents
1. NANOPHOTOMETER®AT A GLANCE.......................................................................9
NANOPHOTOMETER®OVERVIEW ..............................................................................................9
NANOPHOTOMETER®N120...............................................................................................................10
NANOPHOTOMETER®NP80 ..............................................................................................................11
NANOPHOTOMETER®N60.................................................................................................................12
NANOPHOTOMETER®N50.................................................................................................................13
NANOPHOTOMETER®C40.................................................................................................................14
INSTRUMENT REAR PANEL (N120/NP80/N60/N50/C40)....................................................................15
INSTRUMENT BOTTOM VIEW (N120/ NP80/N60/N50/C40) .................................................................15
ACCESSORIES.......................................................................................................................16
STANDARD ACCESSORIES.................................................................................................................16
OPTIONAL ACCESSORIES..................................................................................................................16
CONNECTIVITY.......................................................................................................................20
NANOPHOTOMETER®SPECIFICATIONS ....................................................................................22
2. GETTING STARTED..............................................................................................24
SPECTROPHOTOMETER INSTALLATION ....................................................................................24
SAFETY INFORMATION ......................................................................................................................24
UNPACKING AND POSITIONING ..........................................................................................................25
SOFTWARE INSTALLATION......................................................................................................26
NPOS OVERVIEW.............................................................................................................................26
REQUIREMENTS AND COMPATIBILITY.................................................................................................26
INSTALLING SOFTWARE ON COMPUTER .............................................................................................27
INSTALLING NANOPHOTOMETER®APP ON TABLET OR SMARTPHONE..................................................28
FIRST STEPS AND CONFIGURATION WIZARD ......................................................................................29
PRINTER INSTALLATION ....................................................................................................................29
3. NANOPHOTOMETER®BASICS..............................................................................30
APPLICATIONS OVERVIEW......................................................................................................30
ICONS...................................................................................................................................32
BUTTONS..............................................................................................................................33
MEASUREMENT SCREENS ......................................................................................................34
SINGLE SAMPLE METHODS ...............................................................................................................34
MULTI SAMPLE METHODS .................................................................................................................36
DATA PROCESSING DIALOGS .................................................................................................42
PRINT ..............................................................................................................................................42
SAVE ..............................................................................................................................................44
DELETE............................................................................................................................................46
STORE METHODS ..................................................................................................................47
BASIC OPERATION.................................................................................................................48
NANOVOLUME SINGLE SAMPLE MEASUREMENT BASICS (N50/N60/NP80)..........................................48

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NANOVOLUME MULTI SAMPLE MEASUREMENT BASICS (N120)...........................................................49
CUVETTE MEASUREMENT BASICS (C40/NP80)..................................................................................51
SUBMICROLITER CELL MEASUREMENTS BASICS (C40).......................................................................52
SAMPLE HANDLING TIPS...................................................................................................................53
SOLVENT COMPATIBILITY (N50/N60/N120/NP80/C40 WITH SUBMICROLITER CELL).............................55
DATA TRANSFER VIA FILE SERVER....................................................................................................56
BATTERY OPERATION............................................................................................................57
4. NANOPHOTOMETER®APPLICATIONS ...................................................................58
NUCLEIC ACIDS ..................................................................................................................58
METHOD OVERVIEW..........................................................................................................................58
MEASUREMENT PROTOCOL SINGLE SAMPLE......................................................................................58
MEASUREMENT PROTOCOL MULTI SAMPLE (N120)............................................................................62
CALCULATIONS ................................................................................................................................65
PROTEIN UV ......................................................................................................................70
METHOD OVERVIEW..........................................................................................................................70
MEASUREMENT PROTOCOL SINGLE SAMPLE......................................................................................71
MEASUREMENT PROTOCOL MULTI SAMPLE .......................................................................................74
CALCULATIONS ................................................................................................................................78
PROTEIN ASSAYS ...............................................................................................................84
METHOD OVERVIEW..........................................................................................................................84
MEASUREMENT PROTOCOL SINGLE SAMPLE......................................................................................85
MEASUREMENT PROTOCOL MULTI SAMPLE .......................................................................................88
SAVING AND LOADING STANDARD CURVES........................................................................................93
CALCULATIONS ................................................................................................................................93
KINETICS ..........................................................................................................................94
METHOD OVERVIEW..........................................................................................................................94
MEASUREMENT PROTOCOL...............................................................................................................94
CALCULATIONS ................................................................................................................................96
OD600 ..........................................................................................................................97
METHOD OVERVIEW..........................................................................................................................97
MEASUREMENT PROTOCOL...............................................................................................................98
CALCULATIONS ..............................................................................................................................100
MORE APPS .....................................................................................................................101
MORE APPS:WAVELENGTH ..............................................................................................101
METHOD OVERVIEW........................................................................................................................101
MEASUREMENT PROTOCOL.............................................................................................................101
CALCULATIONS ..............................................................................................................................104
MORE APPS:WAVESCAN .................................................................................................105
METHOD OVERVIEW........................................................................................................................105
MEASUREMENT PROTOCOL SINGLE SAMPLE....................................................................................105
MEASUREMENT PROTOCOL MULTI SAMPLE .....................................................................................107
CALCULATIONS ..............................................................................................................................110
MORE APPS:ABSORBANCE RATIO ...................................................................................111

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METHOD OVERVIEW........................................................................................................................111
MEASUREMENT PROTOCOL.............................................................................................................111
CALCULATIONS ..............................................................................................................................113
MORE APPS:CONCENTRATION .........................................................................................114
METHOD OVERVIEW........................................................................................................................114
MEASUREMENT PROTOCOL.............................................................................................................114
CALCULATIONS ..............................................................................................................................117
MORE APPS:STANDARD CURVE ......................................................................................118
METHOD OVERVIEW........................................................................................................................118
MEASUREMENT PROTOCOL.............................................................................................................118
CALCULATIONS ..............................................................................................................................121
CUSTOM APPS .................................................................................................................121
STORED RESULTS ............................................................................................................122
STORED METHODS ...........................................................................................................123
5. PREFERENCES..................................................................................................124
GENERAL............................................................................................................................124
DATE AND TIME..............................................................................................................................124
DISPLAY ........................................................................................................................................125
ABOUT...........................................................................................................................................125
STORAGE.......................................................................................................................................125
LANGUAGE.....................................................................................................................................125
ILLUMINATION SAMPLE WINDOW .....................................................................................................126
ENABLE NANOVOLUME...................................................................................................................126
DYES 126
WARNING MESSAGES ..........................................................................................................128
BLANK CONTROL ...........................................................................................................................128
SAMPLE QUALITY CONTROL............................................................................................................128
NETWORK...........................................................................................................................129
NETWORK SETTINGS ......................................................................................................................129
WLAN SETTINGS ...........................................................................................................................130
FILE SERVER ACCESS ....................................................................................................................131
NETWORK FOLDER.........................................................................................................................132
PRINTER .............................................................................................................................133
NETWORK PRINTER ........................................................................................................................133
REPORT CONFIGURATION ...............................................................................................................134
CFR21...............................................................................................................................134
6. TROUBLESHOOTING..........................................................................................135
INITIALIZATION TEST.............................................................................................................135
MESSAGES .........................................................................................................................135
IMPORTANT WARNING MESSAGES:..................................................................................................135
IMPORTANT ALERT MESSAGES: ......................................................................................................137

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SYSTEM FREEZE..................................................................................................................138
7. ASSISTANCE.....................................................................................................139
SUPPORT............................................................................................................................139
REPORT PROBLEM ..............................................................................................................139
USER MANUAL ....................................................................................................................140
SOFTWARE MAINTENANCE ...................................................................................................140
NPOS UPDATE...............................................................................................................................140
CREATE LOG FILE ..........................................................................................................................141
FACTORY RESET ............................................................................................................................141
CREATE BACKUP............................................................................................................................141
DIAGNOSTICS......................................................................................................................142
REMOTE ACCESS ................................................................................................................142
LICENSE INFORMATION ........................................................................................................143
END-USER LICENSE AGREEMENT (EULA) ............................................................................143
TRADEMARKS......................................................................................................................143
CONTACT IMPLEN................................................................................................................144
8. MAINTENANCE..................................................................................................145
MAINTENANCE FREE TECHNOLOGY.......................................................................................145
REPLACEMENT PARTS .........................................................................................................145
CLEANING AND GENERAL CARE ...........................................................................................146
9. WARRANTY.......................................................................................................147
10. APPENDIX 1- INSTALACE SPEKTROFOTOMETRU .................................................148
BEZPEČNOSTNÍ INFORMACE.................................................................................................148
VYBALENÍ A UMÍSTĚNÍ ..........................................................................................................149
11. APPENDIX 2-INSTALLATIE SPECTROFOTOMETER ..............................................150
VEILIGHEIDSINFORMATIE ......................................................................................................150
UITPAKKEN EN PLAATSEN ....................................................................................................151
12. APPENDIX 3-INSTALLATION DU SPECTROPHOTOMÈTRE.....................................153
INFORMATIONS DE SÉCURITÉ ................................................................................................153
DÉBALLAGE ET POSITIONNEMENT .........................................................................................154
13. APPENDIX 4–INSTALLATION SPECTROPHOTOMETER.........................................156
SICHERHEITSINFORMATIONEN...............................................................................................156
AUSPACKEN UND PLATZIEREN..............................................................................................157
14. APPENDIX 5–INSTALLAZIONE DELLO SPETTROFOTOMETRO ..............................159
INFORMAZIONI DI SICUREZZA.................................................................................................159

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DISIMBALLAGGIO E COLLOCAZIONE.......................................................................................160
15. APPENDIX 6–INSTALACJA SPEKTROFOTOMETRU..............................................162
INFORMACJE DOTYCZĄCE BEZPIECZEŃSTWA..........................................................................162
ROZPAKOWYWANIE I UMIEJSCOWIENIE ..................................................................................163
16. APPENDIX 7–INSTALACIÓN DEL ESPECTROFOTÓMETRO....................................165
INFORMACIÓN DE SEGURIDAD ...............................................................................................165
DESEMBALAJE E INSTALACIÓN .............................................................................................166
17. APPENDIX 8–SPEKTROFOTOMETERINSTALLATION............................................168
SÄKERHETSINFORMATION ....................................................................................................168
UPPACKNING OCH PLACERING..............................................................................................169
18. APPENDIX 9–INSTALAÇÃO DE ESPECTROFOTÓMETRO ......................................171
INFORMAÇÕES DE SEGURANÇA ............................................................................................171
DESEMBALAMENTO E POSICIONAMENTO ...............................................................................172
19. ALPHABETICAL APPENDIX.................................................................................174

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1.NANOPHOTOMETER®AT A GLANCE
NANOPHOTOMETER®OVERVIEW
The Implen NanoPhotometer®is a mobile, simple to use UV/Visible spectrophotometer with
a CCD array detector with options ranging from NanoVolume to standard cuvette. The
NanoPhotometer®family comprises five different models.
There are four single sample models available:
The NP80 spectrophotometer is a combination of NanoVolume and cuvette option, the N60
and N50 are NanoVolume spectrophotometers only and the C40 is a standard cuvette
solution with the option to upgrade to NanoVolume measurements.
The NanoPhotometer®N120 is a multi-sample spectrophotometer which can measure up to
12 NanoVolume samples simultaneously.
The NanoPhotometer®runs on a Linux-based operating system (NPOS) that is designed for
the use of pre-programmed and custom applications with a high degree of flexibility and
processing power.
Sample Compression Technology™provides easy sample handling which is independent of
surface tension. This technology squeezes the sample between two quartz surfaces allowing
for unmatched precision and accuracy without the need for dilutions. Combined with our
True Path Technology™ the system offers lifetime accuracy and precision without the need
for maintenance or recalibration.
Note: It is recommended to use a properly calibrated pipette with high-quality tips to ensure
delivery of appropriate sample volumes for NanoVolume sample applications.
Sample Control™ is a quality control technology that identifies air bubbles, sample
impurities, turbidity, lint residues and potential contaminations. Sample Control™ monitors
handling characteristics and sample quality in real time to ensure that the measured
concentrations are reproducible and most precise.
Blank Control™ gives a warning message for blanks with high background. High background
absorbance can be caused by a contaminated blank, blank buffer or by residues from
previous users. Insufficient blank readings are the main cause for inaccurate measurements.
Blank Control™ will protect the user from wasting time and precious sample on inaccurate
readings caused by high background blanks or inappropriate cleaning. For more detailed
information see also Technical Note #1 Blank Control™.

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NANOPHOTOMETER®N120
Item Number
Touchscreen
Battery Pack
N120-Touch
+
-
N120-Mobile
+
+

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NANOPHOTOMETER®NP80
Item Number
Touchscreen
Battery Pack
NP80
-
-
NP80-Touch
+
-
NP80-Mobile
+
+

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NANOPHOTOMETER®N60
Item Number
Touchscreen
Battery Pack
N60
-
-
N60-Touch
+
-
N60-Mobile
+
+

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NANOPHOTOMETER®N50
Item Number
Touchscreen
Battery Pack
N50
-
-
N50-Touch
+
-

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NANOPHOTOMETER®C40
Item Number
Touchscreen
Battery Pack
C40
-
-
C40-Touch
+
-
C40-Mobile
+
+

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INSTRUMENT REAR PANEL (N120/NP80/N60/N50/C40)
To boot/shut down the NanoPhotometer®push shortly (< 1 second) on the power on/off
button at the back of the NanoPhotometer®.
Note: A long push (> 3 seconds) initiate a hard reset. Only activate a hard reset of the
NanoPhotometer®when necessary. To avoid unnecessary hard reboots, it is recommended
to power down the unit from the onboard touch screen by pushing on the power button ( ) in
the bottom left corner of the home screen.
INSTRUMENT BOTTOM VIEW (N120/ NP80/N60/N50/C40)
Model name, device serial number and FCC ID are located on the identification plate on the
bottom of the instrument.

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ACCESSORIES
STANDARD ACCESSORIES
Connecting Cable
USB connecting cable to connect the NanoPhotometer®to a computer to control the
NanoPhotometer®via computer (please see page 26 Software Installation).
NanoPhotometer®Power Adapter
Power adapter for the NanoPhotometer®and for charging the optional built-in battery pack.
Note: Use only the power adapter supplied with your instrument or a replacement part from
the manufacturer or your supplier.
Dust Cover
OPTIONAL ACCESSORIES
Didymium Glass Filter (C40/NP80)
The certified didymium glass filter can be used to verify
the wavelength and photometric accuracy of the
NanoPhotometer®cuvette applications.

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Standard Solution (N120, NP80, N60, N50)
The NanoPhotometer®is recalibration free therefore it is
not necessary to review the photometric accuracy on a
regular basis. If the SOPs of a laboratory require a
routine control of the photometric accuracy standard
solutions can be used.
Note: Specifications of the standard solution are guaranteed for at least one year. Please
see expiration date. Once a vail is opened it can be used for 30 minutes.
Note: Please read the Material Safety Data Sheet carefully prior to using this product.
IQ/OQ documentation (N120, NP80, N60, C40)
The NanoPhotometer®IQ/OQ package consists of a Standard Solution and/or a Didymium
Glass Filter and a corresponding Excel-based software tool depending on the type of
instrument. The Excel-based software produces an automated report displaying all relevant
data for an audit.
Note: The IQ/OQ documentation is not available for the NanoPhotometer®N50.
Submicroliter Cell (C40 only)
The submicroliter cell provides the option of upgrading the
NanoPhotometer®C40 to a device that handles NanoVolume
samples as well as cuvette samples. The sample volume range
of the submicroliter cell ranges from a minimum of 0.3 µl to 5 µl
with the path length options including 0.04 mm, 0.1 mm, 0.2
mm, 1 mm and 2 mm which correspond to virtual dilution
factors of 1:250, 1:100, 1:50, 1:10, and 1:5 respectively.
DiluCell™(C40/NP80 only)
The DiluCell™is a disposable cuvette with shortened path
length for virtual dilution of cuvette-based samples. Due to the
reduced path length the DiluCell™provides an automatic
dilution without the need of a physical dilution of higher
concentration samples. There are two different sizes of
DiluCells available DC 10 and DC 20 which allow an
automatic 1/10 and 1/20 dilution of the sample respectively.
Bypassing manual sample dilutions reduces dilution errors
and cross-contamination making DiluCell™ideal for GLP.
Combined with small sample volume requirements and
bubble free filling, the DiluCell™allows for convenient
spectrophotometric analysis from 340 - 950 nm.

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Field Kit
Implen aluminum roller case for safe transportation, designed to fit in most airplane
overhead bins; special compartments for all necessary accessories, cleaning tools and
samples.
Note: Delivered w/o NanoPhotometer®and accessories
Note: Operate the NanoPhotometer®in the roller case only when cover is open. Ensure that
air circulation is possible. Always switch NanoPhotometer®off for transportation.
Barcode Reader
It is possible to import sample names from 1D and 2D barcodes. Connect a compatible
barcode reader to the USB port of the NanoPhotometer®and push on the sample name
input window. After scanning a barcode the sample name will be shown in the input window.
The imported name can then be edited or replaced completely.
Barcode readers that have been tested and verified compatible:
1D: Honeywell Voyager 95X0 Single-Line Laser Scanner
Datalogic Touch65
1D & 2D: AGPtEK SC36
Honeywell Xenon 1900g
DYMO Label Printer
It is possible to connect a DYMO Label printer to the NanoPhotometer®for direct printing on
standard or cryo labels. Recommended and tested printers are the DYMO LabelWriter 4XL
(label size 10.3 x 15.8 cm) and the DYMO LabelWriter 450 (5.4 x 10 cm). Cryo labels can be
printed with both DYMO printers using the following label format: 26 x 12.7 mm and 9.5 mm
circle (landscape).
Note: After starting the NanoPhotometer®, plug in the DYMO printer. The home screen will
be shown. Wait at least for 30 seconds for driver installation.
Cryo labels (26 x 12.7 mm and 9.5 mm circle):
Note: Orientation of cryo labels on carrier foil needs to be landscape.

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Printout for DYMO LabelWriter 4XL Printout for DYMO LabelWriter 450
Note: The printouts are optimized for the label size of the DYMO LabelWriter 4XL. All other
DYMO printers can be used. However, the font size will be zoomed to utilized paper size.
HP Printer
Printing from the NanoPhotometer®is possible via USB (HP printers) and through network
connection. Network printing should be possible with AirPrint / IPP compatible printers
supporting PDF format. For network printer settings see page 133 Network Printer.
Note: IPP version 2.2 is required and some printer configuration settings might need to be
changed in order to allow communication with the NanoPhotometer®
The following HP printers have been tested and deemed compatible to print via USB
connection:
HP LaserJet 3030
HP LaserJet m1522nf MFP
HP LaserJet 400 color M451nw
HP DeskJet 2543
HP DeskJet 1110
Further HP printers are available upon request.
Note: After starting the NanoPhotometer®, plug in the HP printer. The home screen will be
shown. Wait at least for 30 seconds for driver installation.

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CONNECTIVITY
USB A
There is a USB A port on the front as well as the rear panel of the NanoPhotometer®which
is compatible with standard portable USB 2.0 storage devices (back) and USB 3.0 (front) for
direct data transfer in a variety of formats including Excel. It is also possible to connect a
mouse, keyboard, barcode reader, DYMO printer or HP printer directly to the
NanoPhotometer®.
Note: We recommend using FAT/FAT32 formatted 2.0 USB flash drives. The USB flash
drive size is due to the standard formatting currently limited to 32 GB. Encrypted USB flash
drives are not compatible with the NanoPhotometer®.
Note: Cordless Bluetooth mice are not supported. Use wired mice only.
Note: Connect mouse and keyboard before starting the NanoPhotometer®.
USB B
There is a USB B port located on the rear panel of the instrument which is compatible with
the USB cable provided to connect the NanoPhotometer®to a computer. This USB
connection can be used to control the NanoPhotometer®via computer.
LAN
There is an Ethernet (LAN) connection port on the rear panel of the instrument which
enables the NanoPhotometer®to connect with the local network. This Ethernet connection
can be used for data transfer from the NanoPhotometer®to a local network, to control the
NanoPhotometer®via a control device and network printing.
Data transfer is possible by saving on a defined network folder (see page 132 Network
Folder) or on the NanoPhotometer®file server (see page 56 Data Transfer via File Server).
Note: Plug in the LAN cable before starting the NanoPhotometer®.
Note: The maximum LAN cable length is 10 meters. Bit rate is 1 Gbit/s
WiFi
The NanoPhotometer®is equipped with WiFi, which can be used as a WiFi network or as a
WiFi Hotspot. The WiFi network allows same functionality as the Ethernet connection
including direct printing via AirPrint / IPP compatible printers supporting PDF format.
Note: IPP version 2.2 is required and some printer configuration settings might need to be
changed in order to allow communication with the NanoPhotometer®
The WiFi Hotspot provides the option to control the NanoPhotometer®by other WiFi devices
like computer, tablets or smartphones (tablets and smartphones not for N120).
WiFi Hotspot connection details:
SSID: NanoPhotometer®serial number
Password: Implenuser