Invitrogen EVOS XL User manual

For Research Use Only. Not for use in diagnostic procedures.
EVOS™XL Core Imaging System
For transmitted light applications
Catalog Number AMEX1000
Publication Number MAN0007989
Revision B.0

Information in this document is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR
SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM
THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history:MAN0007987
Revision
Date
Description
B.0 16 October 2017 Update branding, legal/regulatory language, reorganize content.
A.0 10 October 2014 Correct standard items included table, update legal and regulatory boilerplate, and
logos.
1.0 8 April 2013 New user guide.
Important Licensing Information: This product may be covered by one or more Limited Use Label Licenses. By use of this
product, you accept the terms and conditions of all applicable Limited Use Label Licenses.
Manufacturer: Life Technologies Corporation | 22025 20th Ave SE St #100 | Bothell, WA 98021
Trademarks: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Kimwipes is
a registered trademark of Kimberly-Clark Corporation.
© 2017 Thermo Fisher Scientific Inc. All rights reserved.

3
Contents
About this guide .............................................................................................................................................. 5
1. Product information ................................................................................................6
Product description......................................................................................................................................... 6
Standard items included................................................................................................................................. 6
Instrument exterior components and manual controls.............................................................................. 7
Descriptions of manual controls.................................................................................................................... 8
Onscreen controls ............................................................................................................................................ 9
Descriptions of onscreen controls ............................................................................................................... 10
2. Installation ............................................................................................................ 11
Operating environment ................................................................................................................................ 11
Transport the EVOS™XL Core to the installation site.............................................................................. 11
Connect power supply.................................................................................................................................. 12
Connect wireless mouse and USB flash drive ........................................................................................... 12
Optional: Install attachable mechanical stage............................................................................................. 13
Install the glass stage plate........................................................................................................................... 13
Install the EVOS™XL Core Imaging System in a cell culture hood........................................................ 14
3. Operation............................................................................................................... 15
Basic operation............................................................................................................................................... 15
Adjust instrument settings........................................................................................................................... 17
Update software ............................................................................................................................................ 18
4. Care and maintenance ..........................................................................................19
General care.................................................................................................................................................... 19
Objective lens care......................................................................................................................................... 19
Stage care........................................................................................................................................................ 19
Decontamination procedures....................................................................................................................... 20
Appendix A: Troubleshooting...................................................................................... 21
Image issues ................................................................................................................................................... 21
Mechanical issues .......................................................................................................................................... 22
Appendix B: Specifications .........................................................................................23
Appendix C: Install the attachable mechanical stage ................................................. 24

4
Appendix D: Safety......................................................................................................26
Safety conventions used in this document................................................................................................. 27
Symbols on instruments ............................................................................................................................... 28
Safety labels on instruments ........................................................................................................................ 30
General instrument safety ............................................................................................................................ 31
Chemical safety.............................................................................................................................................. 32
Chemical waste safety................................................................................................................................... 33
Electrical safety .............................................................................................................................................. 34
Physical hazard safety .................................................................................................................................. 35
Biological hazard safety................................................................................................................................ 35
Safety and Electromagnetic Compatibility (EMC) Standards ................................................................. 36
Documentation and support .......................................................................................37

5
About this guide
Audience
This user guide is for laboratory staff operating, maintaining, and analyzing data
using the EVOS
™
XL Core Imaging System.
User attention
words
Two user attention words appear in Thermo Fisher Scientific user documentation.
Each word implies a particular level of observation or action as described below.
Note: Provides information that may be of interest or help but is not
critical to the use of the product.
IMPORTANT! Provides information that is necessary for proper
instrument operation, accurate installation, or safe use of a chemical.
Safety alert words
Four safety alert words appear in Thermo Fisher Scientific user documentation at
points in the document where you need to be aware of relevant hazards. Each
alert word—IMPORTANT, CAUTION, WARNING, DANGER—implies a
particular level of observation or action, as defined below:
IMPORTANT! – Provides information that is necessary for proper
instrument operation, accurate installation, or safe use of a chemical.
CAUTION! – Indicates a potentially hazardous situation that, if not
avoided, may result in minor or moderate injury. It may also be used to
alert against unsafe practices.
WARNING! – Indicates a potentially hazardous situation that, if not
avoided, could result in death or serious injury.
DANGER! – Indicates an imminently hazardous situation that, if not
avoided, will result in death or serious injury. This signal word is to be
limited to the most extreme situations.
Except for IMPORTANT! safety alerts, each safety alert word in a Thermo Fisher
Scientific document appears with an open triangle figure that contains a hazard
symbol. These hazard symbols are identical to the hazard symbols that are affixed
to Thermo Fisher Scientific instruments (see “Safety symbols”, page 29).
SDSs
The Safety Data Sheets (SDSs) for any chemicals supplied by Thermo Fisher
Scientific are available to you free 24 hours a day. For instructions on obtaining
SDSs, see “Safety Data Sheets (SDS)”, page 37.
IMPORTANT! For the SDSs of chemicals not distributed by Thermo
Fisher Scientific contact the chemical manufacturer.

6
1. Product information
Product description
EVOS
™
XL Imaging
System
The Invitrogen™EVOS™XL Core Imaging System (Cat. No. AMEX1000) is a fully
automated, digital, inverted imaging system. The system is designed for a broad
range of transmitted light applications including time-lapse imaging, manual-
assist cell counting, and image review.
Product use
For Research Use Only. Not for use in diagnostic procedures.
Standard items included
Before setting up your new EVOS™XL Core Imaging System, unpack the unit and
accessories, and verify all parts are present. Contact your distributor if anything is
missing. Damage claims must be filed with the carrier; the warranty does not
cover in-transit damage.
Note: If you do not have your distributor information, contact Technical
Support (page 37).
•
EVOS™XL Core Imaging System, includes fixed
stage (see Note below)
•Wireless mouse
•Power adaptor
•USB flash drive (includes User Guide and Quick
Start Guide)
•Condenser shield, removable
•Glass stage plate (packed in bubble wrap)
•Dust cover
Condenser shield,
removable
Note: The attachable Mechanical stage is an optional feature available
separately from Thermo Fisher Scientific. For ordering information, contact
your distributor or Technical Support (page 37).
IMPORTANT! Wiping the computer supplied with the EVOS™XL Core
Imaging System (i.e., erasing the hard drive to remove all programs, files,
and the operating system) voids the product warranty. Do not install third
party software or update the operating system.

7
Instrument exterior components and manual controls
Phase turret Coarse focus knob
Fixed stage with glass stage plate Fine focus knob
Freeze button Power switch
Save button Power input jack
Illumination wheel USB ports
Objective turret
Mechanical stage
controls
The attachable mechanical stage is an optional feature. See “Appendix C: Install the
attachable mechanical stage” (page 24) for installation and removal.
Stage clip
Stage Y-axis knob
Stage X-axis knob
(mechanical stage)

8
Descriptions of manual controls
This glossary is not alphabetized. Manual controls are listed in the order they are
normally used, with a separate section for the optional Attachable Mechanical
Stage (page 25).
Power input jack
Plug the power adapter into the power input jack.
Power switch
Set the power switch to “ | ” to turn the instrument on or “O” to turn it off.
USB ports
Plug the mouse and USB flash drive into the USB ports.
Illumination wheel
Use the illumination wheel to control illumination intensity.
Coarse & Fine focus
knobs
Use the coarse focus knobs and fine focus knobs to bring the sample into focus.
Coarse focus tension
control ring
Turn the coarse focus tension control ring clockwise (toward the back of the
instrument) to tighten the tension. Turn the ring counterclockwise to loosen the
tension.
Objective turret
Turn the objective turret to change magnifications. The objective turret clicks into
place at each position.
Phase turret
Set the phase turret to the position that corresponds with your selected objective
for transmitted light observations. The selector will click into place for each of the
following positions:
•BF (for brightfield observations)
•4/10 PH (for phase observations at 4× or 10×)
•20/40× PH (for phase observations at 20× or 40×)
Freeze & Save
buttons
Use the Freeze button to capture an image before saving and return to live view
after saving. The Save button saves the file with the current settings (Quick Save
base file name and selected image format).
Mouse scroll wheel
(not shown)
Unless the cursor is over a window, the mouse scroll wheel can control
illumination intensity. Roll the scroll wheel away from you for more illumination
or toward you for less.

9
Onscreen controls
Note: Roll over the bottom of screen with the mouse to activate the
onscreen controls. Onscreen controls are only available when a mouse is
installed.
Login button Scalebar
Illumination slider Settings button
Freeze/Live toggle Onscreen clock
Save image button

10
Descriptions of onscreen controls
This glossary is not alphabetized. Onscreen items are listed from left to right.
Illumination slider
The illumination slider controls the illumination
intensity when the mouse is installed. To turn off
illumination, slide all the way to the left.
Freeze/Live button
The Freeze/Live button toggles between Freeze and Live settings.
Click Freeze to capture an image before saving it. Click Live to
return to real-time viewing.
Save button
The Save button saves the current frozen image to USB
flash drive.
•The icon is gray when no USB is plugged in.
•A green USB icon indicates that a flash drive has been
inserted.
•A red USB icon indicates that a save is in progress and
the flash drive should not be removed.
•After the file is saved, the USB icon turns back to green
and the flash drive is safe to remove.
Settings button
The Settings button displays or hides the Settings window. See “Adjust
instrument settings” (page 17) for an itemized explanation of the Settings
window options.
Virtual keyboard
The virtual keyboard window
allows text entry for file names.
Click Accept when you have
finished entering the file name.
Click Clear to reset the text
field.
Onscreen clock
The onscreen clock shows date and time and can easily be set.
Double-click on the time to reveal the settings and use the +or –
icons to increase or decrease the values. Click Set to save
changes.