Jasco RI-2031 User manual

P/N: 0302-0555A
MODEL RI-2031
INTELLIGENT REFRACTIVE INDEX
DETECTOR
INSTRUCTION MANUAL

i
Safety Considerations
To ensure operation safety, this instrument must be operated correctly and
maintained regularly according to schedule. Carefully read to fully understand
all safety precautions in this manual before operating the instrument. This
manual denotes precautions against actions that can result in hazardous
situations or equipment damage by using the signal words WARNING,
CAUTION, and Note.
(1) Safety symbols
Instruction manual symbol. If the product is marked with this
symbol, refer to the instrument manuals to protect the
instrument against damage.
WARNING A WARNING indicates a potentially hazardous situation which,
if not avoided, could result in death or serious injury.
CAUTION A CAUTION indicates a potentially hazardous situation which, if
not avoided, may result in minor or moderate injury. It may also
be used to alert against unsafe practices.
Do not proceed beyond a WARNING or CAUTION notice until
you understand the hazardous conditions and have taken the
appropriate steps.
Note A Note provides additional information to aid the operator in
obtaining optimal instrument performance.
Pressurized, hazardous solvents are used in high-performance liquid
chromatography. Always follow the proper laboratory procedures to ensure
operator safety. Always wear goggles, gloves and protective clothing when
operating the instrument, especially when injecting a sample and opening
valves.

ii
(2) Warning Labels
Warning labels are attached at several locations on this instrument. Do not
remove, deface or damage the warning labels. If a warning label peels off the
instrument or becomes illegible, contact your local JASCO distributor for a
replacement label. Be sure to indicate the part number (P/N) on the label.
(1) Fuse and Ground Warning Label (P/N: 0822-0163A)
Figure 1 Figure of the side view

iii
Regulatory Statements
CE Notice
Marking by the symbol indicates compliance of this JASCO system to the
EMC (Electromagnetic Compatibility) and Low Voltage Directives of the
European Community. This symbol indicates that this JASCO system meets
the relevant basic safety and health requirements of the EC Directive based on
the following technical standards:
• EN55011 -- ”Limits and Methods of Measurement of Radio Interference
Characteristics of Information Technology Equipment.” -- Group 1, Class A.
• EN61000-6-1 -- "Electromagnetic compatibility (EMC) Part 6-1:Generic
standards – Immunity for residential, commercial and light-industrial
environments"
• IEC61000-3-2 -- "Electromagnetic compatibility (EMC) Part 3-2:Limits –
Limits for harmonic current emissions (equipment input current up to and
including 16A per phase) "
• IEC61010-1 -- "Safety requirements for electrical equipment for
measurement, control and laboratory use – Part 1:General requirements"
• A "Declaration of Conformity" in accordance with the above standards has
been made and is on file at JASCO EUROPE srl, Via Confalonieri 25, 22060
CREMELLA (LC), Italy.
FCC Statement (for USA only)
Federal Communications Commission Radio Frequency Interference
Statement
Warning
This is a Class A product. In a domestic environment this product may cause radi
o
interference, in which case the user may be required to take adequate measures.
Warning
This equipment generates, uses, and can radiate radio frequency energy. If it is not
installed and used in accordance with the instruction manual, it may cause
interference to radio communications. It has been tested and found to comply with the
limits for a Class A computing device pursuant to Part 15 of FCC Rules, which are
designed to provide reasonable protection against such interference when operated in
a commercial environment. Operation of this equipment in a residential area is likely to
cause interference, in which case the user at his own expense will be required to take
whatever measures may be required to correct the interference.

iv
Preface
This instruction manual serves as a guidebook for using this instrument. It is
intended to instruct first-time users on how to properly use the instrument, and
to serve as a reference for experienced users.
Before using the instrument, please read this instruction manual carefully, and
make sure that the contents are fully understood. This manual should be easily
accessible to the operator at all times during instrument operation. When not using
the instrument, keep this manual stored in a safe place. Should this instruction
manual be lost, order a replacement from your local JASCO distributor.

v
Installation Conditions
To ensure safe operation, the following recommendations should be observed:
(1) Do not operate the instrument under voltage fluctuations exceeding
10% of the recommended line voltage. Large fluctuations may cause
the instrument to fail.
(2) Use a three-pronged electrical outlet with a ground. When only a
two-pronged socket is available, use an adapter and be sure to
connect the ground wire of the adapter.
(3) Operate the instrument under a temperature range of 10 ∼30°C.
(4) Operate the instrument under a humidity range of 35 ∼85% (RH).
If ambient humidity exceeds 85% (RH), water vapor may deteriorate
optical components. If possible, install the instrument in a location
having a humidity of 60% or lower.
(5) Operate the instrument under an atmospheric pressure of 750 ∼
1060 hPa.
(6) Avoid strong magnetic fields and sources of high-frequency waves.
The instrument may not function properly when near strong
magnetic fields or high-frequency wave sources.
(7) Avoid vibrations caused by vacuum pumps, electric motors,
processing equipment and machine tools.
(8) Avoid dust and corrosive gas. Do not install the instrument in a
location where it may be exposed to dust, especially in locations
exposed to outside air or ventilation outlets that discharge dust
particles.
(9) Do not install the instrument in a location where it may be exposed to
direct sunlight.
(10) Do not install the instrument in a location where it may be directly
exposed to the air current from an air conditioner or heater, as such
a location may inhibit stable measurement.
Note: The above conditions do not ensure optimal performance of this
instrument.

vi
Maintenance
Consult your local JASCO distributor regarding maintenance. In addition,
contact your local JASCO distributor when transporting the instrument.
Replacement parts can be ordered according to part number from your local
JASCO distributor. When the part number is not known, inform your local
JASCO distributor of the part name, instrument model name and its serial
number.
Notices
(1) Jasco shall not be held liable, either directly or indirectly, for any
consequential damage incurred as a result of product use.
(2) Software prohibitions:
yCopying of software or related materials for purposes other than
backup is prohibited.
yTransfer or authorization of the use of Jasco software to or by a third
party is prohibited.
yDisclosure of confidential information related to Jasco software is
prohibited.
yModification of Jasco software is prohibited.
yUse of Jasco software on multiple workstations or terminals, through
a network or through any other means, is strictly prohibited. (This
does not apply to entities with the network license contract)
(3) The content of this manual is subject to change without notice in
accordance with product improvements.
(4) Unauthorized copying of this manual is prohibited.
(5) This manual shall not be used to guarantee or copyright industrial rights or
other rights.
(6) Company and product names listed herein are trademarks or registered
trademarks of their respective owner.

vii
Warranty
This product is warranted for a period of one year from the date of delivery. If
any defects should occur in the product during this period of warranty, JASCO
will repair or replace the defective part(s) or product free of charge.
This warranty does not apply to defects as a result of the following:
(1) USE FOLLOWING IMPROPER OR INADEQUATE INSTALLATION.
(2) IMPROPER OPERATION.
(3) MOVEMENT, MODIFICATION, OR REPAIR BY PERSONS OTHER
THAN AUTHORIZED JASCO PERSONNEL.
(4) USE OF PARTS OTHER THAN THOSE THAT ARE AUTHORIZED BY
JASCO.
(5) INORDINATELY RAPID DETERIORATION DUE TO THE USE OF
CORROSIVE SOLVENTS OR SAMPLES.
(6) NATURAL DISASTERS SUCH AS FIRES, WATER DAMAGE, OR
EARTHQUAKES.
In addition, this warranty does not cover:
(7) CONSUMABLE PARTS OR PARTS THAT HAVE A SEPARATE
WARRANTY OR A WARRANTY PERIOD THAT IS DIFFERENT THAN
THAT SPECIFIED ABOVE.
The warranty period for all parts and repairs supplied under this warranty
expires with the warranty period of the original product.
Jasco Corporation

viii

ix
Table of Contents
Safety Considerations ...................................................................i
Regulatory Statements................................................................iii
Preface..........................................................................................iv
Installation Conditions .................................................................v
Maintenance .................................................................................vi
Warranty ......................................................................................vii
Table of Contents.........................................................................ix
1. Outline and Specifications...................................................1
1.1 Outline .................................................................................................... 1
1.2 Specifications ........................................................................................ 2
2. Part Names and Functions ..................................................4
2.1 Front panel ............................................................................................. 4
2.2 Operation panel ..................................................................................... 7
2.3 Back panel.............................................................................................. 8
3. Power On/Off and Self-diagnostics...................................10
3.1 Power on and self-diagnostics ........................................................... 10
3.2 Power off .............................................................................................. 11
4. Operations in Normal Operation Mode .............................12
4.1 Parameter changes.............................................................................. 12
4.1.1 Range and response changes..................................................... 12
4.1.2 Temperature control ON/OFF and temperature setting ............ 15
4.1.3 Output polarity change method ([SHIFT][6]) .............................. 15
4.2 Purge valve open/close (Solvent replacement in the reference cell)
............................................................................................................. 17
4.3 Auto-zero operations........................................................................... 18
4.4 Error and warning messages during monitoring.............................. 18

x
4.5 Measurement of concentrated sample (Refractive index range
shifting................................................................................................. 19
5. [SHIFT] Key Operations .....................................................21
5.1 Operation summary............................................................................. 21
5.2 Preamplified output display ([SHIFT][1]) ........................................... 22
5.3 Auto-zero operation setting ([SHIFT][3])............................................ 23
5.4 Zero point shift for integrator output ([SHIFT][4])............................. 24
5.5 Lamp operation time ([SHIFT][5]) ....................................................... 26
5.6 Signal filtering mode ([SHIFT][7])....................................................... 27
5.7 Other settings ([SHIFT][MARK]) ......................................................... 27
5.7.1 Marker voltage/polarity changes................................................. 29
5.7.2 Setting to continue/stop self-diagnostics when an error occurs
................................................................................................................ 30
6. Program Mode Operations.................................................32
6.1 Operation summary............................................................................. 32
6.2 Switching between normal operation mode and program mode .... 32
6.3 File number setting (file loading) ....................................................... 33
6.4 Program editing ................................................................................... 34
6.4.1 Initial parameter editing ............................................................... 34
6.4.2 Time program editing................................................................... 35
6.5 Time program operation...................................................................... 40
6.5.1 Time program execution.............................................................. 40
7. MEASURE RANGE Setting.................................................41
8. Maximum Flow Rate Changing at the Front Panel...........43
8.1 “ ~ 10mL/min” tubing arrangement and solvent replacement method
for reference cell................................................................................. 43
8.2 “10 ~ 50mL/min” tubing arrangement and solvent replacement
method for reference cell................................................................... 44

xi
9. Maintenance........................................................................45
9.1 Error messages and countermeasures.............................................. 45
9.1.1 Errors during self-diagnostics after power on........................... 45
9.1.2 Self-diagnostics during operation .............................................. 46
9.1.3 Error message meanings and countermeasures....................... 46
9.2 Flow cell bubble removal .................................................................... 48
9.2.1 Baselines when bubbles are generated ..................................... 48
9.2.2 Bubble removal method 1 (high flow rate fluid pump).............. 50
9.2.3 Bubble removal method 2 (syringe fluid injection) ................... 50
9.2.4 Bubble removal method 3 (covering tubing outlet)................... 50
9.2.5 Bubble prevention method .......................................................... 50
9.3 Flow cell cleaning method .................................................................. 51
9.4 Calibration method .............................................................................. 52
9.4.1 Tubing ........................................................................................... 53
9.4.2 Standard solution ......................................................................... 53
9.4.3 Replacement of tubing system solvent with water.................... 54
9.4.4 Calibration method....................................................................... 54
9.4.5 Calibration constant confirmation and setting .......................... 56
9.4.6 Alternative calibration method.................................................... 57
9.5 Lamp maintenance time setting ......................................................... 58
9.6 Serial number setting .......................................................................... 59
9.7 Power Fuse replacement..................................................................... 60
10. Appendix.............................................................................62
10.1 Operation Theory............................................................................... 62
10.1.1 Operation theory of deflection differential refractometers ..... 62
10.1.2 Optical system ............................................................................ 63
10.1.3 Signal processing method......................................................... 65
10.1.4 Flow line ...................................................................................... 66
10.1.5 Internal volume of tubing........................................................... 68

xii

1
1. Outline and Specifications
1.1 Outline
The JASCO RI-2031 Intelligent Refractive Index Detector is an HPLC detector
which offers unprecedented versatility, performance, and stability. The RI-2031
features the following:
High stability
High stability is obtained through an optical system that is not easily influenced
by temperature, an efficient thermal exchanger, and a digital signal processing
system.
Wide dynamic range
10 times larger dynamic range than an ordinary HPLC refractive index
detector.
Maximum flow rate of 50mL/min.
Maximum flow rate is 50mL/min. (Solvent: H2O) Wide dynamic range and
Extended Maximum flow rate are applied to the semi-preparation.
Easy operation
Parameters can be easily set using the LCD display, function keys, and
numeric keys. Self-diagnostics results and warning messages are also
displayed, which allows the condition of the instrument to be accurately
monitored. Optical Zero can be performed precisely using the easy key
operation.
Automatic analysis capability
The internal time program allows automatic switching of sensitivity range,
response and other functions. Using LC-Net, parameters can be set from the
Model HSS-2000 System without an interface.
Compact design
The front panel dimensions are the same as other JASCO 2000 series HPLC
equipment (pump, UV detector), which facilitates a neat stacking arrangement.
Safety measures
An internal fluid leak sensor is installed. When a leak from the flow cell occurs,
a signal is output and the pump can be stopped.

2
1.2 Specifications
Model name: RI-2031 Intelligent Refractive Index Detector
Measurement system: Deflection type
Refractive index range: 1.00 ~ 1.75
Measurement range settings: 1/4, 1/2, 1, 2, 4, 8, 16, 32, 64, 128, 256, 512 x
10-6RIU/full scale (“MEASURE RANGE”: STD)
1/4, 1/2, 1, 2, 4, 8, 16, 32, 64, 128, 256, 512 x
10-5RIU/full scale (“MEASURE RANGE”:
WIDE)
Linearity: 5.0 x 10-4 RIU (“MEASURE RANGE”: STD)
5.0 x 10-3 RIU (“MEASURE RANGE”: WIDE)
<Condition> H2O
Noise: 0.2 x 10-8RIU
<Condition> H2O, Flow stopped
Cell capacity: 10µL
Maximum usable flow rate: 10mL/min. (“ ~ 10mL/min.” tubing)
50mL/min. (“10 ~ 50mL/min.” tubing)
<Solvent> H2O
Maximum pressure: 0.1MPa. (“ ~ 10mL/min.” tubing)
0.3MPa. (“10 ~ 50mL/min.” tubing)
Temperature control: PID control by heater,
Setting range: 0 ~ 45°C in 1°C increments.
Control range: Room temperature +10°C ~
Room temperature +25°C
Zero adjustment: Automatic Optical and electronic auto-zero
functions.
Integrator output zero positioning
Reference selection: Electromagnetic valve control
(“ ~ 10mL/min.” tubing)

3
Time program: Parameters that can be set are PURGE
ON/OFF, AUTO ZERO, RANGE, and
RESPONSE. Range from 0.1 to 999.9min.
Response FAST, STD, SLOW (TIME ACCUME, DIGITAL
FICTER)
Input and output signals: Recorder output: 10mV/FS
Integrator output: 5.0 x 10-4RIU/1V(SID)
5.0 x 10-3RIU/1V(WIDE)
Marker and leak output: 1 circuit each
Marker, auto-zero, program reset/start: 1
circuit each
LC-Net control: 1 circuit
Liquid wetted material: Quartz, fluoride resin, stainless steel
(SUS316)
Dimensions and weight: 150(W) x 470(D) x 150(H) mm, excluding
Protrusions.
Approximately 13kg
Power requirements: AC100 ~ 240V, AC, 50/60Hz, 50VA max.
Ambient temperature: +10 ~ +35°C for operation
-30 ~ +60°C for storage
* Specifications are subject to change without notice.

4
2. Part Names and Functions
2.1 Front panel
POWER Switch
Operation Panel
Solvent Inlet and Outlet

Figure 2.1 Front Panel
Name Function
Solvent Inlet and
Outlet
“1” ~ “6”
Operation panel
POWER switch
Sample and Reference In and out connections
Refer to Figure 2.2 and Figure 2.3.
Panel containing control keys, parameter edit keys, and
LCD display showing operating conditions, parameters
and various messages. Refer to Section 4 and 5.
Power switch for the instrument
Note: Suitable tubing for the Solvent Inlet and Outlet connections must be
selected according to maximum flow rate. Before connecting tubing,
refer to Figure 2.2. and Figure 2.3. “ ~ 10mL/min”. tubing is supplied
with the instrument.

5
Note: For a “10 ~ 50mL/min” tubing arrangement, the refractive index signal
output (and display) polarity becomes negative. Change the polarity setting
from plus (+) to minus (-) using the polarity setting function. (Refer to 4.1.3
polarity setting.)
Figure 2.2 “ ~ 10mL/min” flow rate tubing connections
3
4 5 6
2
1 Sample Out (waste)
Sample IN (from column)
Tubing with
OD=1.6mm
ID= 0.8mm
Flow rate: ~10mL/min.

6
Figure 2.3 “10 ~ 50mL/min” flow rate tubing connections
Flow rate: 10 ~50mL/min.
Tubing OD=1.6mm, ID=0.8mm
Replace solvent in the reference cell
by sucking the mobile phase solvent.
Keep the syringe connected during
analysis in order to maintain solvent
in the reference cell and to facilitate
solvent replacement.
Flow rate: 10 ~50mL/min.
Tubing
OD=1.6mm
ID=0.8mm
(Same tubing as used
to connect ports
No.1-2 or ports
No.4-5. for the “ ~
10mL/min” tubing
arran
g
ement.
Tubing
OD=1.6mm
ID=0.8mm
Sample Out
Sample IN
Reference IN
Reference Out
1
Sample Out
Sample IN
Mobile phase
23
4 5
1 2 3
4 5
6
6

7
2.2 Operation panel

Figure 2.4 Operation Panel
Name Function
LCD screen Displays operation conditions, setting values, error
messages and other information.
[PRGM RUN] Used to start and stop the time program. Operation status
is indicated by the lamp to the upper left of the button. The
lamp is lit during program operation.
[AUTO ZERO] Sets the refractive index unit value (RIU) to zero.
[MARK] Outputs a marker signal from the recorder output terminal.
[MONIT] Used to return to the monitor screen from other screens
and to switch between monitor screens.
[SHIFT] Used in combination with other keys to set parameters that
are not frequently changed.
[PURGE] Executes solvent replacement (ON/OFF) for the reference
cell.
[PRGM] Switches between normal and time program mode.
[0] ~ [9], [.] Numeric keys. Keys 1 ~ 5 are used for special functions in
addition to their numeric function. The letters on these keys
describe the special functions. In other words, when editing
parameters, these keys are used to call up the relevant
functions. Refer to sections 4.1.1 and 6.4.2 for details.
[][] keys Used to edit parameters and change screens.
[CLEAR] Used to erase incorrect parameter input and clear error
messages.
[EDIT/ENTER] Used to edit parameters.