Keofitt SESAME series User manual

DON’T GAMBLE WITH YOUR SAMPLE™
SESAME™ SAMPLING VALVE
User Manual

SESAME USER MANUAL V. 4 PAGE 2
DOCUMENT VERSION LOG
The table below lists previous versions of this User Manual and states the major changes between
versions.
Version # Version date Major changes from previous versions
1 September 2016 This is the rst version of the SESAME manual
2 October 2016 Ambient temp. changed in 6.4. Added pressure graphs in 6.7. Spare
parts list in 13.1. 15.2 and 15.4 deleted as not applicable.
3 February 2017 Steel plugs added in 6.3. Chapter 7 Valve Bodies added. Chapter 8
Valve Heads added. Chapter 9 Parts & Accessories added.
4 September 2017 More detailed certicate specications. Expanded temperature
ranges. Modied drawings for Clamp and Varivent connections.
Failure Mode section added to Maintenance chapter. Chapter 14
integrated in chapter 13. Missing drawings/illustrations added.

SESAME USER MANUAL V. 4 PAGE 3
INTRODUCTION:
MANUFACTURER: Keott A/S
Kullinggade 31
5700 Svendborg, Denmark
TYPE: SESAME™ SAMPLING VALVE
PATENTS: DK 175721 B1. CN 100501219C.
EP 1. 690 038 B1. US 7,658,201 B2
YEAR OF INTRODUCTION: 2016
MANUAL LAST UPDATED: September 2017
The English version of this Manual is the governing version and it is the only authorized version. Consequently, KEOFITT
cannot be held liable for other versions including translations of this Manual.

SESAME USER MANUAL V. 4 PAGE 4

SESAME USER MANUAL V. 4 PAGE 5
CONTENTS
1. PRESENTATION ............................................................................................................ 7
1.1 Denition of terms .............................................................................................................................7
1.2 Quick start ..........................................................................................................................................9
2. CLEANING – DISINFECTION – STERILISATION .................................................................. 10
2.1 Clean-In-Place (CIP) .........................................................................................................................10
2.2 Disinfection ......................................................................................................................................10
2.3 Sterilisation ......................................................................................................................................11
3. VALVE DESIGN AND FUNCTIONING................................................................................. 12
3.1 Valve body congurations................................................................................................................12
3.2 Valve surfaces and roughness certicate ........................................................................................12
3.3 Valve head congurations................................................................................................................13
3.4 Rubber caps and steel caps..............................................................................................................13
3.5 Sampling coil.....................................................................................................................................13
3.6 Membranes........................................................................................................................................14
3.7 Parts and Accessories .......................................................................................................................15
3.8 Pressure and vacuum .......................................................................................................................15
3.9 Valve cleaning /disinfection /sterilisation .......................................................................................16
4. EVERYDAY USE OF THE VALVE ....................................................................................... 17
4.1 Batch change cleaning.....................................................................................................................17
4.2 Chemical cleaning, CIP ....................................................................................................................17
4.3 Chemical Disinfection......................................................................................................................18
4.4 Steam sterilisation...........................................................................................................................18
5. VALVE OPERATIONS .................................................................................................... 19
5.1 Chemical CIP ....................................................................................................................................19
5.2 Chemical disinfection ......................................................................................................................20
5.3 Steam sterilisation...........................................................................................................................22
5.4 Sampling ..........................................................................................................................................24
6. TECHNICAL DATA......................................................................................................... 25
6.1 Material .............................................................................................................................................25
6.2 Certicate..........................................................................................................................................25
6.3 Pressure (max.) .................................................................................................................................25
6.4 Temperature......................................................................................................................................25
6.5 Surface nish.....................................................................................................................................26
6.6 Viscosity:............................................................................................................................................26
6.7 Flow ..................................................................................................................................................26
7. VALVE BODIES ............................................................................................................ 28
8. VALVE HEADS.............................................................................................................. 29
9. PARTS & ACCESSORIES................................................................................................. 30
10.MOUNTING INSTRUCTIONS.......................................................................................... 31
10.1 Location...........................................................................................................................................31
10.2 Before welding ................................................................................................................................31

SESAME USER MANUAL V. 4 PAGE 6
11. WELDING INSTRUCTIONS............................................................................................ 32
11.1 Welding method..............................................................................................................................32
11.2 Guideline welding values ...............................................................................................................32
12. BLOCK DIAGRAMS...................................................................................................... 33
12.1 Keott valve type T (tank) ..............................................................................................................33
12.2 Keott valve type P (pipe) ..............................................................................................................33
12.3 Keott valve type clamp connection .............................................................................................34
12.4 Keott valve type Varivent®............................................................................................................34
13. MAINTENANCE .......................................................................................................... 35
13.1 Maintainance...................................................................................................................................35
13.2 Spare parts list ................................................................................................................................35
13.3 Disassembly and assembly of valve body and head.....................................................................36
13.4 Replacing a rubber membrane .....................................................................................................36
13.5 Instructions on replacing a PTFE membrane ...............................................................................37
13.6 Cleaning valve head.......................................................................................................................38
14. FAILURE MODES ....................................................................................................... 39
14.1 Broken membrane tip:...................................................................................................................39
14.2 Broken membrane side:.................................................................................................................39
14.3 Broken integrated o-ring: ..............................................................................................................39
14.4 Cleaning the valve aer a failure:..................................................................................................39
15. MEMBRANES............................................................................................................. 40
15.1 Silicone membrane - art. no. 890051 .............................................................................................40
15.2 EPDM membrane - art. no. 890052.................................................................................................41
15.3 PTFE membrane - art. no. 890055..................................................................................................42

SESAME USER MANUAL V. 4 PAGE 7
1. PRESENTATION
The Keott SESAME sampling valve is an enhancement of the renowned classic W9 sampling valve taking
representative and uncompromised sampling to new heights.
SESAME is a dual function valve individually controlling both the cleaning (steaming or liquid detergent/
disinfectant) process and the sampling action through a single turn knob. In case of steaming steam
supply may be permanently connected and there is no need for a separate steam valve.
From the operator’s point of view SESAME oers an easy and failure-proof handling both when cleaning/
steaming and for sampling.
As with the W9 valve eective cleaning and disinfection/sterilisation of the sampling valve can be
carried out between random samples independently of the course of the production process without
compromising the same. The coaxial design and the electro polished valve interior ensure absolute
cleanability.
Keott valves are used in a wide range of processing industries, such as breweries, dairies, juice/so
drinks and the biotechnological and pharmaceutical industries.
1.1 Denition of terms
In order to ease the reading of this manual and to avoid any misunderstanding, please refer to the
denition of terms in the table below:
TERM DEFINITION
3-A Sanitary Standard 3-A SSI is an independent, not-for-prot US corporation dedicated
to advancing hygienic equipment design for the food, beverage and
pharmaceutical industries.
Acids An acid is a chemical substance whose aqueous solutions are characterized
by a sour taste and the ability to react with bases and certain metals (like
calcium) to form salts. Aqueous solutions of acids have a pH of less than
7. A lower pH means a higher acidity, and thus a higher concentration of
positive hydrogen ions in the solution. Removes limestone and most mineral
deposits.
Alkali Alkalis are all bases, which form hydroxide ions (OH-) when dissolved in
water. The terms “base” and “alkali” are oen used interchangeably. Alkalis
have a pH value above 7. Alkalis dissolves fat and oil, destroys protein and
attacks light metal.
Aseptic sampling The process of withdrawing a sample from the production equipment
through a closed circuit, which has been sterilised and kept sterile with no
exposure to the ambient during the sampling process.
Bioload See Microbial load.
Bioburden See Microbial load.
Chemical Sterilant A few disinfectants will kill spores with prolonged exposure times (3–12
hours); these are called chemical sterilants.
Chlorine Chlorine is a chemical element with symbol Cl and atomic number 17. It
belongs to the halogen group together with for instance iodine. It is a strong
oxidizing agent and reacts with many substances. These properties make
chlorine compounds eicient disinfectants.
CIP Abbreviation of Clean-In-Place. The process of cleaning a process component
(like a sampling valve) without removing it from the production line.
Cleaning Removal, usually with detergent and water or enzyme cleaner and water, of
adherent visible soil on a surface.

SESAME USER MANUAL V. 4 PAGE 8
Complexing agent A substance capable of forming a complex compound with another material
in solution. Improves the cleaning properties of a detergent.
Contact time The time span during which the item is in contact with the detergent or the
disinfectant.
Enzymes Molecules, which are added to cleaning agents to ease the removal
of specic organic material. Assures same cleaning eect at a lower
temperature.
Disinfectant Usually a chemical agent that destroys harmful microorganisms but might
not kill bacterial spores.
Disinfection Thermal or chemical destruction of microorganisms. Disinfection is
less lethal than sterilisation, because it destroys most recognised
microorganisms but not necessarily all microbial forms (e.g. bacterial
spores).
Detergent A cleaning agent that has no antimicrobial eect, but in diluted solutions
good cleaning properties.
EHEDG Abbreviation for the European Hygiene Engineering and Design Group.
EHEDG is a consortium of equipment manufacturers, food industries,
research institutes as well as public health authorities promoting safe
food by improving hygienic engineering and design in all aspects of food
manufacture.
Electro polishing Electro polishing is an electrochemical process by which the high points
within the microscopic surface texture are removed and the corners rounded.
This results in Reduced Product Adhesion, Ease of Cleaning and Improved
Corrosion Resistance.
Exposure time Period in a sterilisation/disinfection process during which the item is
exposed to the sterilant/disinfectant at the specic sterilisation/disinfection
parameters.
Flow path The path the sample ows from the tank or process equipment to the sample
recipient.
Germicidal The property of an agent to destroy microorganisms.
Microbial load The number and types of viable microorganisms with which an item is
contaminated; also called bioload or bioburden.
Microorganisms Animals or plants of microscopic size. As used in food and pharmaceutical
industries, generally refers to bacteria, fungi, viruses and bacterial spores.
Peracetic acid A commonly used disinfectant, which is eicient at low temperature and
short contact time. Relatively harmless as it decomposes into carbon dioxide
(CO2) and water (H2O).
Process media The product in the process equipment and the product from which a sample
is taken.
Representative sample A sample which when it reaches the laboratory is still identical to the process
media. A sample which is in no way contaminated or altered during neither
the sampling process nor the transport to the laboratory.
Sanitization The application of a chemical agent that reduces the number of bacterial
contaminants to a safe level as judged by the public health authorities. The
oicial sanitizer protocol indicates that 99.999% of the specic test bacteria
be killed in 30 seconds under the conditions of the test.
SIP Abbreviation for Sterilise-In-Place. The process of rendering a process
component (like a sampling valve) sterile without removing it from the
production line.

SESAME USER MANUAL V. 4 PAGE 9
Spores Relatively water-poor resting cells surrounded by an impervious cell wall,
which makes them relatively resistant to disinfectants and sterilants. They
are dangerous as they can survive in adverse conditions and re-emerge as
live bacteria at a later stage.
Sporicidal The property of an agent that kills spores.
Steaming The process of using saturated steam under pressure as the sterilising agent.
Sterile State of being free from all living microorganisms. In practice, usually
described as a probability function, e.g., as the probability of any
microorganism surviving sterilisation being one in one million.
Sterilant A few disinfectants will kill spores with prolonged exposure times (3–12
hours); these are called chemical sterilants.
Sterilisation Validated process used to render an item free of all forms of viable
microorganisms. In a sterilisation process, the presence of microorganisms is
expressed in terms of probability. Although this probability can be reduced to
a very low number, it can never be reduced to zero.
Sterility Assurance Level The probability of a viable microorganism being present on an item aer
sterilisation. Usually expressed as 10–n; a SAL of 10-6 means <1/1 million
chance that a single viable microorganism is present on a sterilised item.
Tensides A tenside is a surfactant that reduces the surface tension of water and
assures a faster and better contact between the detergent and the soil.
1.2 Quick start
The table below gives you an overview of the relevant chapters to read depending on the operations you
want to perform to obtain the required hygienic level.
Required
hygienic level
4.1 Pre- production treatment 4.2 Chemical cleaning CIP 4.3 Chemical disinfection 4.4 Steaming 5.1 Chemical CIP 5.2 Chemical disinfection 5.3 Steam sterilisation 5.4 Sampling
Cleaning    
Disinfection    
Sterilisation    

SESAME USER MANUAL V. 4 PAGE 10
2. CLEANING – DISINFECTION – STERILISATION
2.1 Clean-In-Place (CIP)
Thorough cleaning of the valve is a prerequisite for proper disinfection or sterilisation. Cleaning of the
valve is the removal of any visible residual product, it be organic or inorganic. It may be done using either
steam (continuous steam will eventually lead to sterility; SIP = Sterilise-In-Place) or a suitable liquid
detergent.
Cleaning is the removal of adhering soil from the environment and from the previous sample (to
the extent it has not been removed by the recommended post-sample cleaning). Cleaning is usually
performed by ushing with water followed by a thorough washing with an appropriate detergent and
nished o with a thorough rinsing with water.
Depending on the actual process media the proper detergent must be determined in cooperation with
your usual supplier of detergents. The company Novadan ApS, Kolding, Denmark - www.novadan.dk, has
supplied the generic table below for your convenience.
What to clean for Generic cleaning agents Comments
Fat Alkali and Tensides Heat will facilitate the cleaning process
as the fat melts
Protein Alkali, Acids, Tensides and Chlorine Coagulation and burning when heated,
which makes the product hard to
remove.
Sugar, Salt Water is usually suicient as the
product is water soluble
Sugar caramelises when heated,
turning into a hard sticky substance,
which is diicult to remove
Minerals Acids, Complexing agent Oen seen as lime scale
Biolm Alkali and Chlorine, Peracetic acid,
possibly Enzymes
Biolm is an accumulated mass of
microorganisms that is tightly adhered
to a surface and cannot be easily
removed.
Starch Alkali and Chlorine
2.2 Disinfection
Although CIP removes all visible residues of the process media the valve surfaces will still be
contaminated on a microscopic level. Depending on your actual process media it will be necessary to
carry out a disinfection operation in order to a) reduce the microbial load to an acceptable level (also
referred to as Sanitization) or b) destroy critical microorganisms, but not necessarily all microbial forms
(e.g. bacterial spores).
The disinfection process may be carried out in one of two ways and to dierent levels of disinfection
depending on a) the initial microbial load distribution, b) the required hygienic level and c) the type,
exposure time and concentration of the chemicals used (if using a chemical disinfectant):
• By steaming (in a continued process aer steam cleaning)
• By applying one or more suitable liquid chemical disinfectants
There are a number of chemical disinfectants. It is important to choose the right one, the right
concentration and contact time and the right method for your current application. Your usual supplier of
chemical disinfectants can support you in choosing the right disinfectant for your process media and the
specic group of microorganisms you are aiming at.
The company Novadan ApS, Kolding, Denmark has supplied the table below, as a preliminary indication
of which type of disinfectant to use:

SESAME USER MANUAL V. 4 PAGE 11
Disinfectant
Microbes to inactivate
Halogenes
(Clorine)
Peroxides
(hydrogenperoxid &
peracetic acid)
Alcohol (70%)
Gram-neg bacteria
Salmonella
Campylobacter
E. Coli and others...
Gram-pos bacteria
Listeria
Bacillus cereus
Clostridium and others...
Bacteria spores
Bacillus cereus and others...
Bacteriophage
Yeast
Fungi
Virus
Legend: Eicient Limited eect Little/No eect
NOTE! The nal choice of detergent, disinfectant and method lies with the user, supported by the
supplier of the CIP uids and disinfectants, as it is very much dependant on individual concerns
and circumstances.
2.3 Sterilisation
Sterilisation is a high-level disinfection designed to render the valve free of all forms of viable
microorganisms (incl. bacterial spores) to a high level of certainty; the so-called Sterility Assurance Level
or SAL. A SAL value of 10-6 means that the probability (or risk) of a single viable microorganism being
present on the valve interior aerwards is only 1 in 1,000,000 which is a generally accepted level for
calling an item sterile. Although the probability can be reduced to a very low number, it can never be
reduced to zero.
Sterility may in practise only be obtained by steaming. Disinfectants exist that in high concentrations
and for a prolonged exposure time will be able to inactivate all forms of microorganisms and render the
valve interior sterile with a high probability; these disinfectants are called chemical sterilants. However,
the application of chemical sterilants is most oen problematic due to a) a required high concentration,
which causes an operator hazard and b) the several hours of exposure time.
NOTE! Furthermore, sterilisation with a chemical sterilant may not convey the same sterility as-
surance as sterilisation with steam, because the germicidal and sporicidal kinetics are much less
investigated and documented for chemical sterilants compared to steam.

SESAME USER MANUAL V. 4 PAGE 12
3. VALVE DESIGN AND FUNCTIONING
The valve is designed to regularly take representative samples in the production process. The valve is
therefore designed such that eective cleaning, disinfection/sterilisation and sampling can be carried
out regularly without interrupting the production process.
NOTE! The membrane has a tripple function: it serves as a dynamic seal towards the process side,
it acts as a dynamic seal to activate cleaning/steaming and it constitutes a hygienic static sealing
against the valve head.
3.1 Valve body congurations
Valve bodies may be welded to the process equipment or connected by means of one of the standard
connector systems.
Welding congurations encompass the following options:
• Tank welding, ø38 mm
• Pipe welding, 25 mm
Connector congurations encompass the following options:
• Varivent, ø50 mm
• Varivent, ø68 mm
• Mini Clamp
• Clamp 1”
• Clamp 2”
• Union, DIN 11851
The inlet and outlet ports are available in the following congurations:
• Hose Piece (Keott Quick Coupling)
• Mini Tri-clamp
• Thread M16x1.5
• Welding ends (to weld steel tubing on)
All welding/connector options are available with Hose Piece ports. For the other port congurations
only some combinations are standard; but all non-standard combinations are likely to be available “On
request”.
All valve bodies with their various ports are machined in one piece of steel, thus avoiding all crevices and
ssures from screwed or welded parts.
For further information please consult www.keott.dk.
3.2 Valve surfaces and roughness certicate
All Keott valves are electro polished internally using a proprietary technique and each valve has its
individual certicate (Test Report) stating the results of 3 test measurements of the surface roughness in
each of the 2 ports, in the valve chamber and on the outer body surface with product contact.
For obvious reasons, the surface properties of the internal product contact areas of a sampling valve are
much more important from a hygiene perspective than any shiny outside surface appearance. Therefore,
Keott has developed a unique electrode conguration to assure during manufacturing that the internal
surfaces in particular are properly electro polished.

SESAME USER MANUAL V. 4 PAGE 13
3.3 Valve head congurations
Valve heads come in the following congurations:
• Turn knob (type H)
• Lever handle (type Q)
• Pneumatic (type N)
All congurations may be delivered with PTFE, EPDM or Silicone membranes.
3.4 Rubber caps and steel caps
The purpose of the cap is to protect the valve chamber from the environment between sampling.
Steel caps (part no. 800061) may be used instead of rubber caps and will provide a stronger mechanical
xation to the hose piece and thus be operational under higher pressure and temperature. Furthermore,
the steel plugs may be autoclaved.
For further information please consult www.keott.dk.
3.5 Sampling coil
When sampling beer, the rapid pressure drop from inside the tank (CO2pressure of 2-3 bar) to the open
pressure less sample recipient causes excessive foaming, even when opening the valve very slowly and
very little. This phenomenon may be alleviated connecting a sampling coil (part no. 800058) between the
sampling valve and the sample recipient. A sampling coil provides a slower pressure drop as the sample
ows through the full length of the coil, approx. 1 m.
For further information please consult www.keott.dk.

SESAME USER MANUAL V. 4 PAGE 14
3.6 Membranes
All valve head may be delivered with any of the 3 materials: Silicone, EPDM and PTFE. For FFKM please
contact your local dealer or Keott.
They all have dierent properties and dierent resistance to various chemical substances, as seen in the
table below.
Resistance to - EPDM Silicone PTFE (Teon) FFKM
- weather and Ozone
- hydrolysis (water and
steam)
- to acids and bases
- mineral oil and gas X
X=not suitable =less suitable =Suitable =Very Good =Excellent
Put in words the properties can also be described like this:
• The Silicone membrane has the advantage that it in general can withstand high
temperatures, but it cannot tolerate moisture condensation resulting from steam
sterilisation
• The EPDM membrane is better able to cope with the condensation in the steam and at
the same time it can be used with a majority of CIP uids and disinfectants in normal
concentrations
• The PTFE membrane resists all CIP uids and disinfectants except highly oxidising acids in
high concentrations
• The FFKM covers the highest temperature range and has excellent chemical resistance to
virtually any chemical compound
Membranes in rubber materials like EPDM, Silicone and FFKM are fully interchangeable. This means that
you may change from for instance Silicone to EPDM, if required.
However, valve heads with PTFE membranes are designed dierently from the others. As PTFE is a sti
material with no elastic properties, it cannot be stretched. Therefore, it is made with a bellow to allow for
the valve movement.
NOTE! The membrane functions as a dynamic seal in the valve seat and the CIP/steam seat, as
well as a hygienic static sealing against the valve head.

SESAME USER MANUAL V. 4 PAGE 15
3.7 Parts and Accessories
Keott provide a huge number of spare parts and accessories to the SESAME valves.
These include accessories like:
• Barbed ttings and tube welding ttings for tubes and hoses
• Adaptors between Tri-clamp, Mini Tri-clamp and Hose Piece (Quick Coupling)
• Spike Bag holders and Spikes
• Fitted PTFE tubing for Quick Coupling and Tri-clamp
• Any length of PTFE tube
• Clamps for Tri-clamp connections
• Hypodermic needles
and spare parts like:
• O-rings and gaskets
• Membranes
3.8 Pressure and vacuum
Pressure ratings:
All SESAME valves feature a spring to provide the closing force against the valve seat. The spring is
dimensioned such that all valves must pass a pressure test up to 10 bar. At some tank pressure above 10
bar the spring will give way and the valve will leak.
A nominal max. constant pressure of 6 bar allow suicient tolerance to cater for the most common
pressure peaks in a process line.
Vacuum ratings:
On installations where vacuum will occur, rubber membranes (EPDM, FFKM, Silicone) are at risk of being
sucked hard into the valve seat, whereby the valve might not open properly. However, the additional
(closing) force from the vacuum (corresponding to max. -1 bar(g) or 0 bar(abs)) is rather small (10%)
compared the force exerted by the spring (corresponding to at least 10 bar(g)), so there is no risk of
damaging the membrane as long as the vacuum is only present when the valve is closed.
Besides, attempting to open a sampling valve under vacuum makes no sense, since nothing will ow out,
so the incident is rather improbable.
Rubber membranes will seal perfectly well against vacuum, when the valve is kept closed.
WARNING:
• When opening the valve while the process side is under vaccum there is a risk that the
membrane may be sucked passed the valve seat and into the valve opening, which could cause
the membrane to be damaged.
• On installations where vacuum will occur, PTFE membranes don’t have the risk of being sucked
into the valve seat, but as it is a harder and less exible material a complete tightness against
the ambient air may not be secured.

SESAME USER MANUAL V. 4 PAGE 16
3.9 Valve cleaning /disinfection /sterilisation
The table below describes the two fundamentally dierent ways of preparing the valve for sampling,
1) Chemical cleaning/disinfection and 2) Steaming:
Method Description Pros & Cons
Chemical
Chemical
cleaning
Liquid detergents are used to clean the
valve.
CIP = Clean-In-Place
This process is adopted where steam
is not available or where the product
cannot withstand the exposure to heat.
Involves several stages with ushing,
cleaning and rinsing between batches.
Chemical
disinfection
A disinfection process using an
appropriate chemical liquid disinfectant
usually follows the cleaning process.
The valve interior is wetted, soaked
or ushed with an appropriate
disinfectant.
It adds 2 more stages to the CIP:
application of disinfectant and nal
rinse.
Involves handling of potentially
hazardous chemicals.
Thermal
Sterilisation Steam is supplied for 1 minute just
before and immediately aer sampling.
Steaming does ushing, cleaning,
rinsing and sterilisation in one
operation.
Steaming is not suitable with heat
sensitive products.
Steaming entails the risk of burns.
Flushing with water followed by the supply of a chemical detergent through the inlet port, while in
“Clean” position, results in cleaning the valve (CIP). It is the perfect, hygienic design and surface nish
of the inner part of the valve, which enables easy, eicient and reliable cleaning in a closed state of the
valve.
Supplying steam at the steam INLET port and setting the valve at “Clean” results in cleaning and
sterilisation. It is the perfect, hygienic design and surface nish of the inner part of the valve,
which enables sterilisation in a closed state. According to an EHEDG based test conducted by the
Biotechnological Institute in Denmark, the valve is sterile aer just 1 minute’s supply of steam at a
pressure of 1 bar(g), 121 ºC. Steaming is therefore a SIP process (Sterilise-In-Place)
WARNING
• During sterilisation with steam the valve will become hot and care should thus be taken when
operating the valve
• The valve is designed for use in working conditions of up to 6 bar(g) pressure and temperatures
of up to 121 C. It is therefore important to be aware that the rubber plug (designed for max. 3
bar(g)) or the steel plug (designed for max. 10 bar(g)) may be forced out at high speed, if not
seated properly
• When steaming always use dry saturated steam at 1 bar(g). At higher pressure the membrane
may be damaged/split
• Always remember to use safety goggles when steaming, CIPping, taking samples and all other
operations of the sampling valve

SESAME USER MANUAL V. 4 PAGE 17
4. EVERYDAY USE OF THE VALVE
This chapter gives an introduction to how the sampling valve works in dierent operating conditions.
conditions, such as the cleaning of the entire production line before starting a new batch (chapter 4.1)
and the cleaning of the valve between each sample during the batch production (chapters 4.2-4.4)
For specic operator instructions please refer to the chapter “VALVE OPERATIONS”.
4.1 Batch change cleaning
Before every new production batch the sampling valve is cleaned and disinfected/sterilised together
with the tank or vessel or the entire production line.
There are 3 dierent ways to perform the cleaning between batches as explained in the table below:
TANK
from CIP supply
Tank mounted:
This cleaning method is suitable where the tank is spray cleaned
between batches and a proper CIP ow through the valve cannot
be assured from inside the tank.
Set the valve in “Sample” position and connect a hose from the
CIP supply to the outlet port. In this way the CIP liquid will ow
through the outlet port and into the tank and clean the valve
chamber and the valve seat.
The valve body end surface will be spray cleaned together with the
tank interior.
PIPE
to CIP return pipe
Pipe mounted:
This cleaning method is suitable if the valve is connected to a pipe
with suicient pressure to cause an adequate ow through the
valve.
Set the valve in “Sample” position and connect a hose from the
outlet port to a CIP return pipe.
CIP
from CIP supply
to CIP return pipe
Through CIP port:
This operation is normally not necessary, as this cleaning
procedure will be executed before and aer each sampling.
Nevertheless, if cleaning through the CIP port is requested for the
batch change cleaning the valve must at some time during the CIP
cycle be set in its “Clean” position and hoses to and from the CIP
supply connected as shown in the illustration.
Remember to close the valve aer the nal rinse and prior to starting up the next production batch.
4.2 Chemical cleaning, CIP
During production and prior to sampling, cleaning takes place with the valve closed and involves the
following stages:
1. Pre-rinse
Flushing with water to mechanically remove product residues
2. Clean
Applying a detergent to remove remaining visible product residues
3. Final rinse
Rinse with clean water to remove all traces of detergents

SESAME USER MANUAL V. 4 PAGE 18
Usually this procedure is followed by disinfection (see below), but for some application CIP might be
suicient. It depends on your (microbiological) requirements, the detergents applied and the process
media to clean for. Consult your supplier of CIP uids.
In some cases where the process media is for instance water, CIP might not even be necessary and you
may go directly to disinfection.
4.3 Chemical Disinfection
Disinfection takes place with the valve closed and involves the following stages of which the rst 3 are
identical to CIP:
1. Pre-rinse
Flushing with water to mechanically remove product residues
2. Clean
Applying a detergent to remove remaining visible product residues
3. Intermediate rinse
Rinse with clean water to remove all traces of detergents
4. Disinfection
Apply an appropriate disinfectant targeting one or more or all microorganisms
5. Final rinse
Rinse with cleaned water to remove all traces of the disinfectant
4.4 Steam sterilisation
Steaming has the advantage that it does ushing, cleaning and sterilisation in one operation. However
the heat from the steam will cause sugary substances to caramelise and substances containing protein
to coagulate and burn; see chapter 2.1. In this case you must disconnect any xed steam supply in order
to ush the valve with an appropriate uid prior to the post-sampling steaming.
If steaming is the preferred procedure, but no steam is installed near the sampling point, an option is
to use a portable steam generator. Keott supplies ttings for a Kärcher steam generator. The steaming
process with a Keott sampling valve has been validated to obtain sterility aer 1 minute of steaming at
121° C (1 bar(g)). Documentation is available at the Keott Online Service Center on www.keott.dk.

SESAME USER MANUAL V. 4 PAGE 19
5. VALVE OPERATIONS
This chapter provides clear instructions on how to operate the sampling valve in dierent situations.
Before sampling the valve must be cleaned followed by disinfection or sterilisation, depending on your
requirements.
For the initial cleaning before a new batch please refer to chapter 4.1 Batch change cleaning.
IMPORTANT
• All illustrations show a sampling valve with Keott hose piece connections. All instructions
also apply to valve versions with clamp connections; only make sure to use the corresponding
ttings.
• All illustrations show a sampling valve with a turn knob. However the instructions also apply to
pneumatic valves and valves with a handle.
5.1 Chemical CIP
The CIP takes place with the valve remaining in its closed position.
Perform the following steps:
nPROCESS MEDIA
nCIP LIQUID
nCLEAN WATER
nSTEAM / DISINFECTANT
1. Remove the plugs, if any, from the INLET and OUTLET ports.
In the case of a valve with clamp connections there are no plugs
supplied.
2. Connect a water hose to the INLET hose piece.
3. Connect a hose to the OUTLET hose piece and let the hose go to a
drain.
4. Flush with clean water by turning the knob towards “Clean”.
5. Remove the water hose and let the CIP liquid ow through the
upper hose piece. If the CIP liquid must not go to drain, circulate it
or collect it in a suitable container and dispose of correctly.

SESAME USER MANUAL V. 4 PAGE 20
6. Reconnect the water hose to the upper hose piece and rinse with
clean water by turning the knob towards “Clean”.
If disinfection is not needed the valve is now ready for taking a sample. If disinfection is required proceed
with the steps mentioned in the section “Chemical disinfection” below.
Flush with clean water aer sampling. If the process media is sticky, viscous or aggressive or for any
other appropriate reason, do repeat the full CIP cycle aer sampling.
WARNING
• Carefully follow the guidelines given for the chemicals involved
• Always remember to use safety goggles when steaming, CIPping, taking samples and all other
operations of the sampling valve
5.2 Chemical disinfection
Immediately following the CIP, perform the disinfection, if required. The disinfection takes place with the
valve remaining in its closed position.
There are 2 recommended ways to carry out the disinfection:
A) by letting the disinfectant ow through the valve chamber
B) by lling the valve chamber with the disinfectant (advantage: smaller volume of disinfectant
needed and quicker and more reliable disinfection)
Steps to perform, when adopting A:
nPROCESS MEDIA
nCIP LIQUID
nCLEAN WATER
nSTEAM / DISINFECTANT
1. Connect a hose with an empty bottle to the lower hose piece. This
bottle or similar recipient is to collect the disinfectant (step 3) and
the rinsing water (step 6).
2. Fill a exible bottle with the dened amount of disinfectant.
3. Connect the exible bottle via a hose to the INLET hose piece,
turn the valve towards “Clean” and press the disinfectant slowly
through the valve to wet the interior of the valve.
4. Allow the disinfectant to act for the prescribed time.