7
14
8
9
10
12
11
13
7.1 Zero point calibration
▸ Hold up the refractometer against a sufficiently bright light source and look through
the eyepiece [4], holding the eyepiece closely against your eye/glasses.
▸ Rotate the eyepiece [4]to adjust it for your eyesight, until you can see the scale
sharply.
▸ Open the prism cover [1].
▸ Thoroughly clean the prism [2]and the underside of the prism cover [1]using a so
cloth or so paper (with alcohol if necessary)and wipe dry. Water should only ever
be applied to the measuring prism, not to other parts of the device.
▸ Now apply a few drops of the calibration liquid [10]onto the prism surface [2].
▸ close the prism cover [1]. The amount of fluid should be sufficient to moisten most of
the prism surface. There should not be any air bubbles between the measuring prism
[2]and prism cover [1].
▸ Wait about 30 seconds to allow the temperatures of the test fluid and prism to
equalise.
▸ Look through the eyepiece [4]while pointing the refractometer‘s prism surface [2]at
a bright light source.
▸ Through the eyepiece [4], you will see a bright and a blue field. The boundary line
between them shows the refractive index value on the scale which is also visible
through the eyepiece [4].
▸ With the aid of the supplied adjustment tool [11]to turn the adjustment screw [3]
at the side of the prism surface [2]the scale can be adjusted so that the
measuring device is optimally set up by moving the boundary line upwards or
downwards.
Calibration value: 0 % (Brix)
▸ For refractometers without automatic temperature compensation (ATC): Convert the
measured value to take account of the deviation in room and sample
temperature from +20 °C. (See the table at the end of this manual, Point 14.)
▸ Repeat step 4 (cleaning).
Important!
The ambient/room temperature and the sample temperature influence
the refractometer measuring result.
The scales are designed for an ambient temperature of +20 °C!
If the measurements are carried out at a temperature other than +20
°C, the results must be correspondingly corrected.
A correction table can be found in the annex, Point 14.
7.2 Additional advice
It is important that the samples being measured are representative samples. Meas-
urements should be carried out quickly on samles that evaporate easily.If possible,
the samples should be at the same temperature as the measuring instrument in order
to achieve an accurate result.
7.3 Measuring procedure
▸ Make sure your hands are dry before handling the refractometer.
▸ Open the prism cover [1]and use the supplied pipette [8]to apply a few drops of
the sample liquid [8]onto the prism surface [2], then close the prism cover [1].
Spread the liquid evenly by pressing down on the prism cover [1]and eliminate
any air bubbles present.
▸ Hold the device horizontally and wait about 30 seconds (for optimal temperature
equalisation between the sample and device).
▸ View the measurement scale through the eyepiece [4]. Point the prism surface [2]
of the refractometer at a bright light source while doing this.
▸ Rotate the adjustment ring [5]on the eyepiece [4]to adjust the focus.
▸ The boundary line will move on the measurement scale depending on the concen-
tration. This bright/dark boundary line shows the result directly on the scale.
▸ If the temperature deviates from +20 °C, correct the measured result using the
corresponding value from the temperature correction table [13].
▸ Carefully clean the supplied pipette [8]and the refractometer aer carrying out
the measurement.
Important!
Aer every measurement, use a lint-free, absorbent cloth to remove
the fluids from the prism surface [2]and prism cover [1]. Then
carefully clean the prism and prism cover using a cloth moistened
with water and dry both parts using a so, dry and lint-free cloth. Avoid rubbing the
prism [2].
9
8. Cleaning and maintenance
with water, or if necessary alcohol. Do not use any aggressive or abrasive
cleaning agents. Never immerse the device in water or hold it under running water.
Never handle the device with wet or damp hands.
Never touch the measuring prism [2]with hard tools made from plastic, wood,
glass, resulting in measurement errors.
The refractometer is maintenance-free.
refractometer to maximise its life and optimise measurement results.
9. Storage
Store the refractometer in a dry, non-corrosive environment, preferably
between 10 °C and 30 °C.
10. Service
Aer reading this operating manual, if you have any questions about setting up or
using the refractometer, or if any unexpected problem occurs, please contact your
dealer. The device housing may only be opened by trained service technicians
authorised by KERN.
11. Disposal
The packaging consists of environmentally friendly materials which can be disposed
of via local recycling facilities.
The device and storage box should be disposed of by the operator in accordance
with applicable national or regional regulations at the place of use.
12. Additional information
The product may differ slightly from the illustrations.
Avoid exposing the refractometer to direct sunlight.
Never bring the refractometer into contact with solvents.
13. Brix to refractive index (nD) conversion table
Data from „ICUMSA“ International Commission for Uniform Methods
of Sugar Analysis, at 20 °C and 589 nm wavelength.
!
!
14. Annex
Table 1: International Temperature Correction Table for °Brix (% sugar gradient)
Correct the result by the following values (refractometer must be correctly
calibrated at 20 °C).
6 1.34175361.39220661.45584
7 1.34325371.39409671.45822
8 1.34476381.39600681.46061
9 1.34629391.39792691.46303
101.34782401.39986701.46546
111.34937411.40181711.46792
121.35093421.40378721.47037
131.35250431.40576731.47285
141.35408441.40776741.47535
151.35568451.40978751.47787
161.35729461.41181761.48040
171.35891471.41385771.48295
181.36054481.41592781.48552
191.36218491.41799791.4881
201.36384501.42009801.49071
211.36551511.42220811.49333
221.36720521.42432821.49597
231.36889531.42647831.49862
% Brix
reading
0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0
Temperature °C
10.0 -0.53 -0.56 -0.59 -0.62 -0.65 -0.67 -0.69 -0.71 -0.72 -0.73 -0.74 -0.75 -0.75 -0.75 -0.75 -0.75 -0.74 -0.73
11.0 -0.49 -0.52 -0.54 -0.57 -0.59 -0.61 -0.63 -0.64 -0.65 -0.66 -0.67 -0.68 -0.68 -0.68 -0.68 -0.67 -0.67 -0.66
12.0 -0.44 -0.47 -0.49 -0.51 -0.53 -0.55 -0.56 -0.57 -0.58 -0.59 -0.60 -0.60 -0.61 -0.61 -0.60 -0.60 -0.60 -0.59
13.0 -0.40 -0.41 -0.43 -0.45 -0.47 -0.48 -0.50 -0.51 -0.52 -0.52 -0.53 -0.53 -0.53 -0.53 -0.53 -0.53 -0.52 -0.52
14.0 -0.34 -0.36 -0.38 -0.39 -0.40 -0.42 -0.43 -0.44 -0.44 -0.45 -0.45 -0.46 -0.46 -0.46 -0.46 -0.45 -0.45 -0.44
15.0 -0.29 -0.31 -0.32 -0.33 -0.34 -0.35 -0.36 -0.37 -0.37 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.37 -0.37
16.0 -0.24 -0.25 -0.26 -0.27 -0.28 -0.28 -0.29 -0.30 -0.30 -0.30 -0.31 -0.31 -0.31 -0.31 -0.31 -0.30 -0.30 -0.30
17.0 -0.18 -0.19 -0.20 -0.20 -0.21 -0.21 -0.22 -0.22 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.22
18.0 -0.12 -0.13 -0.13 -0.14 -0.14 -0.14 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15
19.0 -0.06 -0.06 -0.07 -0.07 -0.07 -0.07 -0.07 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.07
20.0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
21.0 0.06 0.07 0.07 0.07 0.07 0.07 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.07
22.0 0.13 0.14 0.14 0.14 0.15 0.15 0.15 0.15 0.16 0.16 0.16 0.16 0.16 0.16 0.15 0.15 0.15 0.15
23.0 0.20 0.21 0.21 0.22 0.22 0.23 0.23 0.23 0.23 0.24 0.24 0.24 0.24 0.23 0.23 0.23 0.23 0.22
24.0 0.27 0.28 0.29 0.29 0.30 0.30 0.31 0.31 0.31 0.32 0.32 0.32 0.32 0.31 0.31 0.31 0.30 0.30
25.0 0.34 0.35 0.36 0.37 0.38 0.38 0.39 0.39 0.40 0.40 0.40 0.40 0.40 0.39 0.39 0.39 0.38 0.37
26.0 0.42 0.43 0.44 0.45 0.46 0.46 0.47 0.47 0.48 0.48 0.48 0.48 0.48 0.47 0.47 0.46 0.46 0.46
27.0 0.50 0.51 0.52 0.53 0.54 0.55 0.55 0.56 0.56 0.56 0.56 0.56 0.56 0.55 0.55 0.54 0.53 0.52
28.0 0.58 0.59 0.60 0.61 0.62 0.63 0.64 0.64 0.64 0.65 0.65 0.64 0.64 0.64 0.63 0.62 0.61 0.60
29.0 0.66 0.67 0.68 0.69 0.70 0.71 0.72 0.73 0.73 0.73 0.73 0.73 0.72 0.72 0.71 0.70 0.69 0.68
30.0 0.74 0.75 0.77 0.78 0.79 0.80 0.81 0.81 0.81 0.82 0.81 0.81 0.81 0.80 0.79 0.78 0.77 0.75