Labconco FreeZone 794801 Series User manual

Original instructions
Models
794801*** Series

Original instructions
Copyright © 2018 Labconco Corporation. The information contained in this manual and the accompanying
products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves
the right to make periodic design changes without obligation to notify any person or entity of such change.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier.
Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery
carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar with
any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,
land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to
user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525
or 1-816-333-8811, between the hours of 7:30 a.m. and 5:30 p.m., Central Standard Time.
Part #7343500, Rev. F
ECO N983
Warranty
Labconco Corporation provides a warranty to the original buyer for the repair or replacement of parts and
reasonable labor as a result of normal and proper use of the equipment with compatible chemicals. Broken
glassware and maintenance items, such as filters, gaskets, light bulbs, finishes and lubrication are not
warranted. Excluded from warranty are products with improper installation, erratic electrical or utility supply,
unauthorized repair and products used with incompatible chemicals.
The warranty for FreeZone®Stoppering Tray Dryers will expire one year from date of installation or two years
from date of shipment from Labconco, whichever is sooner. Warranty is non-transferable and only applies to
the owner (organization) of record.
Buyer is exclusively responsible for the set-up, installation, verification, decontamination or calibration of
equipment. This limited warranty covers parts and labor, but not transportation and insurance charges. If the
failure is determined to be covered under this warranty, the dealer or Labconco Corporation will authorize
repair or replacement of all defective parts to restore the unit to operation. Repairs may be completed by 3rd
party service agents approved by Labconco Corporation. Labconco Corporation reserves the rights to limit this
warranty based on a service agent’s travel, working hours, the site’s entry restrictions and unobstructed access
to serviceable components of the product.
Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages
of any kind. This warranty is exclusive and in lieu of all other warranties whether oral, or implied.

Original instructions
TABLE OF CONTENTS
CHAPTER 1: INTRODUCTION 1
Intended Use 1
Freeze Dry Process 1
Freeze Dry Rates 2
Freeze Dry Capacity 3
Samples Containing Volatile Substances 4
About This Manual 4
Typographical Conventions 5
CHAPTER 2: PREREQUISITES 6
Electrical Requirements 6
Location Requirements 7
Vacuum Pump Requirements 7
CHAPTER 3: GETTING STARTED 8
Unpacking Your Stoppering Tray Dryer 8
Stoppering Tray Dryer Components 9
Setting Up Your Stoppering Tray Dryer 9
Installing Stoppering Tray Dryer on a Console Freeze Dryer 9
Electrical Connection 10
Communication Cable 11
Ground Strap 11
Sample Probes 11
Testing System Vacuum Integrity 11
Venting the Vacuum Pump 12
Chemical Resistance of Freeze Dryer Components 12
Solvent Safety Precautions 13
CHAPTER 4: USING YOUR STOPPERING TRAY DRYER 14
Component Identification 15
Operation Checklist 16
Operating the Stoppering Tray Dryer 17
Drying Accessory Screen 17
Manual Mode 18
Starting Manual Mode 19
Changing Shelf Temp Set Point Value 19
Stopping Manual Mode 20

Original instructions
Programs 20
Creating a New Program 22
Starting a Program 23
Stopping a Program 23
Freeze Drying Using the Manifold Valves 24
Shelf Spacing Adjustment 26
Shelf Loading 27
Sample Freezing 27
Freeze Drying 28
Stoppering 28
Vacuum Break/Backfilling 28
Alerts 29
Power Fail 29
Line Voltage Range 29
System Temp Variation 29
Vacuum Variation 29
System Temp Set Point 30
Program Complete 30
CHAPTER 5: MAINTAINING YOUR STOPPERING TRAY DRYER 31
Service Safety Precautions 31
Routine Maintenance Schedule 31
CHAPTER 6: TROUBLESHOOTING 33
Vacuum Pump 33
Gaskets, Tubing, Connections, Sample Valves 34
System Components & Collector Chamber Isolation 35
Stoppering Tray Dryer 37
Door Adjustment 37
Refrigeration Module Operation 38
Access to Mechanical Components 38
Shelf and Stoppering System Removal 39
Filling the Fluid System 40
Heat Transfer Fluid Precautions 41
Heat Transfer Fluid First Aid Procedures 42
APPENDIX A: COMPONENTS 43
APPENDIX B: DIMENSIONS 45
APPENDIX C: SPECIFICATIONS 47
Electrical Specifications 47
Environmental Conditions 47
APPENDIX D: ACCESSORIES 48

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Original instructions
CHAPTER 1
INTRODUCTION
Congratulations on your purchase of a Labconco FreeZone® Freeze Dry System,
which is designed for laboratory lyophilization procedures. The refrigerant used
in the refrigeration system is HCFC-free so it will not endanger the environment.
The unit is easy to install and maintain. Proper care and maintenance of this
product will result in many years of dependable service.
Intended Use
The Stoppering Tray Dryer is intended to be used in conjunction with Labconco
Console Freeze Dryers in a laboratory setting to facilitate the lyophilization
process.
Freeze Dry Process
Freeze drying is an important process in sample preparation and for the preservation
and storage of biologicals, pharmaceuticals and foods. Of the various methods of
dehydration, freeze drying (lyophilization) is especially suited for substances that are
heat sensitive. Other than food processing (e.g., coffee, whole dinners), freeze drying
has been extensively used in the development of pharmaceuticals (e.g., antibiotics)
and preservation of biologicals (e.g., proteins, plasma, viruses and cell lines). The
nondestructive nature of this process has been demonstrated by the retention of
viability in freeze dried viruses and microorganisms.
Freeze drying is a process whereby water or other solvent is removed from frozen
material by converting the frozen water directly into vapor without the
intermediate formation of liquid water. The basis for this sublimation process
involves the absorption of heat by the frozen sample in order to vaporize the ice;
the use of a vacuum pump to enhance the removal of water vapor from the surface
of the sample; the transfer of water vapor to a collector; and the removal of heat
by the collector in order to condense the water vapor. In essence, the freeze dry
process is a balance between the heat absorbed by the sample to vaporize the
ice and the heat removed from the collector to convert the water vapor into ice.

Chapter 1: Introduction
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Original instructions
Freeze Dry Rates
The efficiency of the Freeze Drying process is dependent upon the surface area
and the thickness of the sample, the collector temperature and vacuum obtained,
the eutectic point and solute concentration of the sample. It is important to
remember these factors when trying to obtain efficient utilization of your Freeze
Dry system. A listing of selected materials and their approximate drying times
are shown in Table 1 for your reference.
SAFE TEMPERATURE AND DRYING TIMES
FOR SELECTED MATERIALS
Material
10mm Thick
Safe
Temperature
°C
Collector
Temperature
°C
Hours
(Approx.)
Milk
-5
-40
10
Urea
-7
-40
10
Blood Plasma
-10 to -25
-40
16
Serum
-25
-40
18
Vaccinia
-30 to -40
-50
22
Influenza Vaccine
-30
-50
24
Human Tissue
-30 to -40
-50
48
Vegetable Tissue
-50
-80
60
*Total sample quantities are contingent on various freeze dryer capacities.
Up to the point of overloading the system, the greater the surface area of the
sample, the faster the rate of freeze drying. By contrast, for a given surface area,
the thicker the sample the slower the rate of freeze drying. This is based on the
fact that the heat of sublimation is usually absorbed on one side of the frozen
sample and must travel through the frozen layer to vaporize water at the other
surface. In addition, as the sample is freeze dried, the water vapor must travel
through the layer of dried material. The thicker the sample, the greater the chance
that the dried layer may collapse which would cause an additional decrease in the
rate of freeze drying.
The surface area and thickness of the sample can usually be ignored when each
sample contains only a few milliliters. However, for larger volumes, the samples
should be shell frozen to maximize the surface area and minimize the thickness of
the sample. The volume of the freeze dry flask should be two to three times the
volume of the sample.
In order for lyophilization to occur, ice must be removed from the frozen sample
via sublimation. This is accomplished by the collector and the vacuum pump.
The collector, which should be at least 15 to 20°C colder than the eutectic
temperature (melting temperature) of the sample, traps vapor as ice. Since the
vapor pressure at the collector is lower than that of the sample, the flow of water
vapor is from the sample to the collector. Since this vapor diffusion process
occurs very slowly under normal atmospheric conditions, a good vacuum is
Table 1

Chapter 1: Introduction
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Original instructions
essential to maintain an efficient rate. In many applications, the maintenance of a
vacuum of 0.133 mbar or less is recommended.
The rate of freeze drying is directly proportional to the vapor pressure and the
vapor pressure is dependent upon both eutectic temperature and solute
concentration of the sample. For example, a solution of sodium chloride and
water would freeze dry at a slower rate than pure water. The eutectic temperature
of a sodium chloride solution is about –21°C and at this temperature the vapor
pressure is about 1/16 that of water at 0°C. Although the eutectic temperature is
not dependent upon the concentration of sodium chloride, the vapor pressure of
the water would decrease as the concentration of sodium chloride increased. This
is due to the fact that as the solute concentration increases, less of the surface area
of the frozen sample is occupied by water. In general, most solutions or
biological samples will have a eutectic temperature of –10 to –25°C. However, if
the sample contains a simple sugar such as glucose or if the sample is animal or
plant tissue, the eutectic temperature may be as low as –30° to –50°C.
Freeze Dry Capacity
The volume of a sample that can be freeze dried at one time is related to factors
discussed previously and the size and design of the freeze dry system. With any
given instrument, the capacity is based on the surface area of the sample; the
eutectic temperature and concentration of the sample; and the rate and amount of
heat transferred to the frozen sample. Of these factors, the eutectic temperature is
the most important factor in determining the amount of sample that can be freeze
dried at one time, particularly when flasks are used. This is because as the
eutectic temperature decreases, the vapor pressure decreases but the rate of heat
absorption by the sample does not change. This tends to promote melting of the
sample, which leads to a marked increase in vapor pressure and ultimately
overloads the collector and vacuum pump. Samples that have eutectic
temperatures of –20°C or lower should be placed on the freeze dry system one
flask at a time so that the vacuum in the system may recover before adding
another sample to the system. If the vacuum does not recover, the capacity of the
freeze dry system has been exceeded and the sample should be removed.
If there is a problem with a particular type of sample melting when placed on the
freeze dry system, dilution of the sample with more water or providing some
insulation around the flask to decrease the rate of heat absorption by the sample
may help. If the eutectic temperature of the sample is –40 to –60°C, the freeze
dry system selected for use must be equipped with cascade type refrigeration so
that the collector temperature can be cooled to below –75°C, or a dry ice/solvent
trap may be used between the collector and the vacuum pump.

Chapter 1: Introduction
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Original instructions
Samples Containing Volatile Substances
In certain cases the solvent in a sample to be freeze dried may contain volatile
components such as acetonitrile, methanol, acetic acid, formic acid or pyridine.
In addition to these substances having an effect on the eutectic temperature, they
may increase the vapor pressure at the surface of the sample. Also, compared to
water, they will require the absorption of less heat for sublimation to occur.
Hence, samples that contain volatile substances will have a greater tendency to
melt, particularly when placed in flasks or exposed to room temperature. If a
sample containing a volatile substance tends to melt when placed on a freeze dry
system, dilution of the sample with more water will help keep the sample frozen.
For example, a 0.2M solution of acetic acid is much easier to freeze dry than a
0.5M solution.
About This Manual
This manual is designed to help you learn how to install, use, and maintain your
Stoppering Tray Dryer. Instructions for performing routine maintenance and
making minor modifications to your Stoppering Tray Dryer are also included.
Chapter 1: Introduction provides a brief overview of the freeze dry process,
explains the organization of the manual, and defines the typographical
conventions used in the manual.
Chapter 2: Prerequisites explains what you need to do to prepare your site before
you install your Stoppering Tray Dryer. Electrical requirements are discussed.
Chapter 3: Getting Started contains the information you need to properly unpack,
inspect and install your Stoppering Tray Dryer.
Chapter 4: Using Your Stoppering Tray Dryer discusses the basic operation of
your Stoppering Tray Dryer. Information on how to load samples and run the
Stoppering Tray Dryer is included.
Chapter 5: Maintaining Your Stoppering Tray Dryer explains how to perform
routine maintenance on your Stoppering Tray Dryer.
Chapter 6: Using the RS-232 Receptacle describes how to connect a computer
for monitoring the operation.
Chapter 7: Troubleshooting contains information about problems you may
encounter while using your Stoppering Tray Dryer, including the probable causes
of the problems, and suggested corrective actions.
Chapter 8: Modifying Your Stoppering Tray Dryer Display describes how to
calibrate the vacuum display and restore factory settings.

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Original instructions
Appendix A: Components contains labeled diagrams of the components of the
Stoppering Tray Dryer.
Appendix B: Dimensions contains comprehensive diagrams showing the
dimensions for the Stoppering Tray Dryer.
Appendix C: Specifications contains product specifications.
Appendix D: Accessories see www.labconco.com for a complete list of
Stoppering Tray Dryer accessories.
Typographical Conventions
Recognizing the following typographical conventions will help you understand
and use this manual:
•Book, chapter, and section titles are shown in italic type (e.g., Chapter 3:
Getting Started).
•Steps required to perform a task are presented in a numbered format.
•Comments located in the margins provide suggestions, reminders, and
references.
•Critical information is presented in boldface type in paragraphs that are
preceded by the exclamation icon. Failure to comply with the information
following an exclamation icon may result in injury to the user or permanent
damage to your Freeze Dryer.
•Important information is presented in capitalized type in paragraphs that are
preceded by the pointer icon. It is imperative that the information contained
in these paragraphs be thoroughly read and understood by the user.
!
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Original instructions
CHAPTER 2
PREREQUISITES
Before you install your Stoppering Tray Dryer, you need to prepare your site for
installation. The Stoppering Tray Dryer will mount on top of a FreeZone 6, 12 or
18 liter Console Freeze Dryer. Carefully examine the location where you intend
to install your Stoppering Tray Dryer. You must be certain that the area is level
and of solid construction. An electrical source must be located near the
installation site.
Carefully read this chapter to learn:
•Electrical supply requirements.
•Vacuum pump requirements.
Refer to Appendix C: Specifications for complete Stoppering Tray Dryer electrical
and environmental conditions, specifications and requirements.
Refer to the User’s Manual for the FreeZone Console Freeze Dryers for complete
electrical and environmental conditions, specifications and requirements.
Electrical Requirements
•115V models
o20 Amp dedicated single phase circuit.
oProperly grounded receptacle for NEMA
5-20P plug.
•230V models
o15 Amp dedicated single phase circuit.
oProperly grounded receptacle that matches the plug supplied with
the unit, (230V models are supplied with one of the following
plugs):
US
NEMA 5-20P

Chapter 2: Prerequisites
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Original instructions
US EU China UK India
(NEMA 6-15P)
oIf the plug does not match the available receptacle, remove the
plug and replace it with an approved plug of the suitable style.
Do not use any detachable power cord that is not adequately
rated for the unit.
Frequency must agree with the serial tag rating.
Improper frequency will damage the compressor.
ADDITIONAL APPLIANCES ON THE SAME CIRCUIT MAY
CAUSE THE CURRENT TO EXCEED THE RATING OF THE
CIRCUIT BREAKER OR FUSE.
Location Requirements
The Freeze Dryer should be located in an area that provides an unobstructed flow
of air around the cabinet. This air cools the refrigeration system. The
refrigeration system draws air in through the front and exhausts it through the
rear. A minimum of 3" must be allowed between the rear and both sides of the
Freeze Dryer and adjacent wall surfaces. Restriction of airflow during operation
could adversely affect performance. Refer to Appendix B: Dimensions for
dimensional drawings of the Stoppering Tray Dryer.
Vacuum Pump Requirements
A vacuum pump must be provided by the user. A vacuum pump with a
displacement of 163 liters per minute and 0.007 mbar ultimate pressure is
adequate for most samples. The inlet fitting on the vacuum pump must be
suitable for 3/4" ID vacuum hose, which is provided. It is recommended that the
vacuum pump is equipped with an exhaust filter to minimize oil mist exhausting
from the vacuum pump. The operating vacuum level may be set on the Freeze
Dryer. The higher the vacuum set point, the more likely it is that oil mist will be
exhausted.
Vacuum pumps used with 115V models should be equipped with a 115V, 15 Amp
NEMA 5-15P plug. Vacuum pumps used with 230V models should be equipped
with a reverse IEC plug (included with 230V pumps purchased from Labconco).
This will allow the vacuum pump to be plugged into the receptacle on the rear
electrical panel of the Console Freeze Fryer.

!
!

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Original instructions
CHAPTER 3
GETTING STARTED
Now that the site for your Stoppering Tray Dryer is properly prepared, you are
ready to unpack, inspect, install and test your Stoppering Tray Dryer. Read this
chapter to learn how to:
•Unpack and move your Stoppering Tray Dryer.
•Set up your Stoppering Tray Dryer.
•Connect the electrical supply source to your Stoppering Tray Dryer.
•Safely use solvents with your Stoppering Tray Dryer.
The Stoppering Tray Dryer weighs over 400 lbs. (181 Kg). The
carton allows for lifting with a mechanical lift truck or hand
truck. If you must lift the Stoppering Tray Dryer manually,
use at least four (4) persons and follow safe lifting guidelines.
Unpacking Your Stoppering Tray Dryer
Carefully unpack your Stoppering Tray Dryer and inspect it for damage that may
have occurred in transit. If your Stoppering Tray Dryer is damaged, notify the
delivery carrier immediately and retain the entire shipment intact for inspection
by the carrier.
DO NOT RETURN GOODS WITHOUT THE PRIOR
AUTHORIZATION OF LABCONCO. UNAUTHORIZED
RETURNS WILL NOT BE ACCEPTED.
!
The United
States Interstate
Commerce
Commission
rules require
that claims be
filed with the
delivery carrier
within fifteen
(15) days of
delivery.
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Chapter 3: Getting Started
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Original instructions
IF YOUR STOPPERING TRAY DRYER WAS DAMAGED IN
TRANSIT, YOU MUST FILE A CLAIM DIRECTLY WITH THE
FREIGHT CARRIER. LABCONCO CORPORATION AND ITS
DEALERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGE.
DO NOT DISCARD THE CARTON OR PACKING MATERIAL
FOR YOUR STOPPERING TRAY DRYER UNTIL YOU HAVE
CHECKED ALL OF THE COMPONENTS AND INSTALLED
AND TESTED THE STOPPERING TRAY DRYER.
Stoppering Tray Dryer Components
Verify that the components listed below are present and undamaged.
Qty
Part #
Description
Comments
1
794801***
Stoppering Tray Dryer
1
1089005
User’s Manual
USB Flash Drive
1
Power Cord
See parts list (Appendix A)
1
7364601
Communication Cable
3
7365800
Sample Temp Sensor
8
1911416
Washer, ¼”
4
1905621
Nut, ¼ - 20
4
1880712
Screw, ¼ - 20 x .75 long
If you did not receive one or more of the components listed for your Stoppering
Tray Dryer, or if any of the components are damaged, contact Labconco
Corporation immediately for further instructions.
Setting Up Your Stoppering Tray Dryer
After you verify receipt of the proper components, move your Stoppering Tray
Dryer to the location where you want to install it. Then, follow the steps listed
below.
Installing the Stoppering Tray Dryer on a Console
Freeze Dryer
The following tools are required to install the Stoppering Tray Dryer onto a
FreeZone Console Freeze Dry: Flat blade screwdriver or 5/16" socket, and a
7/16" wrench or socket.
1. Remove the four plastic hole plugs from the top of the Freeze Dryer on
which the Stoppering Tray Dryer is to be mounted.
2. If the Freeze Dryer has a flat gasket around the 3.0 inch vacuum port,
remove it.
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Chapter 3: Getting Started
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3. Loosen both clamps on the lower rubber coupling and slide the coupling
upward so the bottom edge of the coupling is above the lower edge of the
stainless steel tube. Snug both clamps on the coupling to hold them in
place.
4. Lift the Stoppering Tray Dryer into place on your Freeze Dryer while
centering the vacuum coupling over the vacuum connection port. Align
the four mounting holes in the Stoppering Tray Dryer support stand with
the four holes in the top of the Freeze Dryer. Install the four bolts, nuts,
and washers provided.
NOTE: Do not lift the Stoppering Tray Dryer by the acrylic door.
5. Loosen the clamps and slide the lower rubber coupling down over the
vacuum connection port. Orient the clamps to gain access and tighten the
clamps to provide a leak-free connection.
Electrical Connection
Plug the power cord into the receptacle on the back of the Stoppering Tray Dryer
and plug the other end into a suitable power receptacle.
DO NOT ATTEMPT TO PLUG THE STOPPERING TRAY
DRYER INTO THE FREEZONE FREEZE DRYER.
CAUTION: When the appropriate power cord is plugged into
the power receptacle on the rear panel of the Stoppering Tray
Dryer, the clamp on the receptacle should be tightened to
prevent the cord from accidentally being switched with the
Freeze Dryer cord located underneath the Tray Dryer. (The
Freeze Dryer may require different mains A.C. voltage and
switching these power cords may cause damage).
Freeze dryer
Stainless steel tube
Stoppering tray dryer 1/4-20 x 3/4 hex head
screw (4) places
1/4" flat washer
(4) places
1/4" flat washer
(4) places
1/4-20 nut w/ integral
lockwasher (4) places
Do not remove this panel
Rubber coupling
Plastic plug
(4) places
Remove flat gasket
if in place on vacuum
connection port
Clamp
!
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Original instructions
Communication Cable
The Freeze Dryer power cord should be unplugged before connecting the
communication cable. Connect one end of the communication cable (7364601) to
the port on the back of the Stoppering Tray Dryer labeled “OUTPUT TO
FREEZE DRYER”. Connect the other end to the port on the back of the Freeze
Dryer labeled “DRYING ACCESSORY”. Plug the Freeze Dryer power cord
back into a suitable power receptacle.
Ground Strap
Connect the Ground Strap by removing one of the screws from the Freeze Dryer
back panel and attaching the free end of the Ground Strap.
Sample Probes
If desired, connect the sample probes into the receptacles inside the chamber.
Testing System Vacuum Integrity
The Stoppering Tray Dryer is now installed and must be tested to make certain the
system is free of leaks. To test, turn on the Freeze Dryer refrigeration
(COLLECTOR) and allow the temperature to reach –40° or lower. Close the
door of the Tray Dryer and make sure the Stoppering control is in the “LOWER”
position and the Vac Release valve is in the “CLOSE” position. Start the vacuum
pump and monitor the vacuum reading on the Freeze Dryer display. The vacuum
should reach 0.133 mbar within 30 minutes and should achieve an ultimate
vacuum of 0.040 mbar or lower within 18 hours. If 0.040 mbar cannot be
achieved, consult the troubleshooting section of this manual and of the manual
supplied with the Freeze Dryer.
Sample
Probes
Connector
clamp
Communication
cable
Ground
Strap

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Original instructions
Venting the Vacuum Pump
If any materials will be placed in the Stoppering Tray Dryer
that can liberate hazardous gases when heated, the vacuum
pump exhaust must be vented to a fume hood or other
ventilation device.
Chemical Resistance of Freeze Dryer
Components
The FreeZone Freeze Dryer and Stoppering Tray Dryer are designed to be
chemically resistant to most compounds that are commonly used in freeze drying
processes. However, by necessity, the Freeze Dryer is comprised of a number of
different materials, some of which may be attacked and degraded by certain
chemicals. The degree of degradation is dependent on the concentration and
exposure duration. Some of the major components of the FreeZone Freeze Dry
System that are susceptible to degradation are as follows:
Acids
Buffers
Solvents
Component
Material
Acetic Acid 20%
Formic Acid
Trifluoroacetic Acid (TFA)
Calcium Chloride
Sodium Phosphate
Acetone
Acetonittirle
Carbon Tetrochloride
Cyclohexane
Dioxane
Methyl t-Butyl Ether (BTBE)
Pyridine
Valve Stem
Acetal
C
D
D
D
D
Collector*
Lid & Door
Acrylic
D
D
D
D
Hoses,
Gaskets &
Valve
Bodies
Neoprene
C
D
D
C
C
D
D
D
C
D
Flask Top
Silicon
Rubber
C
D
D
D
D
D
C
D
Chamber &
Fittings
Stainless
Steel
C
* An accessory glass lid is available for the Freeze Dry Systems.
C –Moderate degradation; Limited use.
D –Severe degradation; infrequent use recommended; immediate
thorough cleaning required.
•Most common compounds used in freeze drying processes, if allowed to enter the
vacuum pump, will degrade the oil and cause damage to the vacuum pump.
!

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Original instructions
•Sugars and proteins typically will have minimal negative effect on any of the
materials of construction.
When using compounds in the Freeze Dryer that are hostile to the materials of
construction, it is imperative the equipment is thoroughly cleaned after use.
•Rubber and plastic components that have been exposed to damaging
compounds should be removed and flushed with water.
•The oil in the vacuum pump should be checked often. It must be changed if it
is cloudy, shows particles or is discolored. The useful life of vacuum pump
oil can be extended if the vacuum pump is operated for an extended period of
time after a freeze dry run. This allows contaminants to be purged from the
hot oil. This must be done with the inlet to the pump blocked off to prevent
air from free flowing through the pump. This is accomplished by closing all
sample valves on a clean, dry freeze dry system and turning on the vacuum
pump. If the pump is operated at an elevated vacuum level (> 10mbar), oil
may be expelled from the pump and damage could occur.
Another way to extend the life of the vacuum pump is to install an optional
secondary trap in the line between the Freeze Dry System and the vacuum pump.
Contact Labconco for ordering information.
With prudent maintenance the FreeZone Freeze Dry System will provide years of
service. Warranty on the affected parts will be voided if maintenance has been
obviously neglected. If you have questions about using specific compounds in the
Freeze Dry System, contact Labconco Technical Service at 1-800-821-5525 or
816-333-8811 or e-mail: [email protected].
Solvent Safety Precautions
Solvents used in the Stoppering Tray Dryer may be flammable
or hazardous to your health. Use extreme caution and keep
sources of ignition away from the solvents. When using
flammable or hazardous solvents, the vacuum pump must be
vented to a fume hood.
Hazardous materials such as strong acids or bases, radioactive
substances and volatile organics must be handled carefully and
promptly cleaned up if spilled. If a sample is spilled in the
collector chamber it must immediately be cleaned up.
WARNING: The disposal of substances used in connection with
this equipment may be governed by various Federal, State or
local regulations. All users of this equipment are urged to
become familiar with any regulations that apply in the user’s
area concerning the dumping of waste materials in or upon
water, land or air and to comply with such regulations.
!

Product Service 1-800-522-7658
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Original instructions
CHAPTER 4
USING YOUR STOPPERING
TRAY DRYER
After your Stoppering Tray Dryer has been installed as detailed in Chapter 3:
Getting Started, you are ready to begin using your Stoppering Tray Dryer. Read
this chapter to learn how to:
•Operate the controls.
•Understand the display.
•Connect samples.
Do not use the Stoppering Tray Dryer in a manner not
specified by the manufacturer (refer to Appendix C:
Specifications). The electrical protection properties of the
Stoppering Tray Dryer may be impaired if the Stoppering
Tray Dryer is used inappropriately.
!

Chapter 4: Using Your Stoppering Tray Dryer
Product Service 1-800-522-7658
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Original instructions
Component Identification
Stoppering
Control Valve
POWER / STANDBY
LED Indicator
IN PROCESS
LED Indicator
Vacuum Release
Valve
Power Switch
(3) Sample Probe Ports
(3) Shelves
Stoppering Bladder
6 Port Manifold (optional)
Backfill Port
Door Latch / Handle

Chapter 4: Using Your Stoppering Tray Dryer
Product Service 1-800-522-7658
16
Original instructions
Power Switch –Turns all AC power to the Tray Dryer ON or OFF.
POWER / STANDBY LED Indicator –Indicates if the power switch is ON or OFF.
IN PROCESS LED Indicator –This light will be ON if the Stoppering Tray Dryer is
running in either MANUAL or PROGRAM mode.
Stoppering Control Valve –Controls the operation of the stoppering bladder. The white
dot on the valve knob indicates the position of the valve. Turning the valve to the
RAISE positon will inflate the bladder to perform the stoppering operation. Turning
the valve to the LOWER position will cause the bladder to deflate.
Vacuum Release Valve - Vents the chamber to allow the chamber door to be opened.
Backfill Port –A regulated gas cylinder may be connected to this port to allow the
introduction of a desired gas into the chamber after freeze drying. The port accepts
1/8” ID tubing.
Ethylene Oxide is not recommended for use in this Tray Dryer for
decontamination because of its hazardous and corrosive properties.
Sample Probe Ports –(3) sample probes may be plugged into the Stoppering Tray Dryer
to monitor the sample temperature during the freeze drying process.
Stoppering Bladder –Can be inflated to perform vial stoppering
Operation Checklist
The following checklist should be followed prior to each use of your Stoppering
Tray Dryer:
1. Using a soft, lint-free cloth, wipe the Stoppering Tray Dryer chamber to
remove any moisture or debris. Wipe door gasket to remove any debris or
other contaminants that could cause a vacuum leak. Vacuum grease is NOT
required on the door gasket to obtain a proper vacuum seal.
2. Remove the collector chamber lid from the Freeze Dryer and ensure that the
collector chamber and drain line are free of water. Place the drain hose in a
suitable container to collect the condensate from the collector chamber and
insert the quick connect drain fitting into the quick connect drain coupling
located on the left hand side of the Freeze Dryer. Freeze Dryer (Collector or
Vacuum) will not start if moisture is detected in the drain line.
3. After completely draining the system, disconnect the quick connect drain
fitting from the quick connect drain coupling.
!