LEEC Culture Safe Touch 190 User manual

Jan 2012 T190 Iss.2 Page 1 of 38
Operating Manual
Culture Safe CO2 Incubators
Touch 190
Touch 190S
Address: LEEC Ltd
Private Road No. 7
Colwick Industrial Estate
Nottingham NG4 2AJ
UK
Tel: [phone]
Fax: [phone]
Enquiries E-mail: [email protected]
Sales E-mail: [email protected]
After Sales E-mail: [e-mail]
Service E-mail: [e-mail]

Jan 2012 T190 Iss.2 Page 2 of 38
CONTENTS
1. SAFETY
1.1. LEGAL CONSIDERATIONS
1.2. INTENDED USE
1.3. SYMBOLS USED IN THIS MANUAL
1.4. GENERAL SAFETY INSTRUCTIONS
2. DESCRIPTION
2.1. CONSTRUCTION
2.2. DOORS
2.3. CONTROL SYSTEM
2.4. CO2 SENSOR
2.5. DIAGRAMS
3. DELIVERY, TRANSPORTATION AND STORAGE
3.1. UNPACKING
3.2. LIFTING AND MOVING
3.3. STORAGE
4. INSTALLATION AND SET-UP
4.1. LOCATION AND AMBIENT CONDITIONS
4.2. SHELVES AND USABLE SPACE
4.3. WATER TRAY
4.4. GAS CONNECTION
4.5. ELECTRICAL CONNECTION
5. GETTING STARTED
5.1. START UP
5.2. SETTING TIME AND DATE
5.3. FACTORY PRESETS
5.4. MAIN DISPLAY
5.5. CHANGING TEMPERATURE SET POINT AND ALARM THRESHOLDS
5.6. CHANGING CO 2 SET POINT AND ALARM THRESHOLDS
5.7. ALARM STATUS SCREEN
6. UTILITY MENU
6.1. GRAPHICAL DISPLAY
6.2. UTILITY MENU
6.3. DOOR ALARM
6.4. INCUBATOR INFO
6.5. HISTORICAL EVENT LOG
6.6. USER SECURITY

Jan 2012 T190 Iss.2 Page 3 of 38
7. SD CARD DATA LOGGING
7.1. START LOGGING
7.2. CHANGING FILENAME
7.3. REMOVING THE SD CARD
8. HIGH TEMPERATURE 200 °C STERILISATION
8.1. PREPARATION
8.2. STARTING THE CYCLE
8.3. STERILISE PHASE
8.4. COOLING PHASE
8.5. ABORTING THE CYCLE
9. ERROR MESSAGE SCREENS
10.OPTIONS
10.1. RS 232 SERIAL INTERFACE
10.2. BMS VOLT FREE ALARM RELAY.
10.3. ETHERNET INTERFACE
10.4. 4-20mA RETRANSMIT
10.5. MULTI-DOORS
11. ACCESSORIES
12.CLEANING
12.1. OUTER SURFACES
12.2. INNER SURACES
12.3. OXIDATION AT HIGH TEMPERATURE
13.SERVICE
13.1. WARRANTY
13.2. PREVENTATIVE MAINTENANCE
13.3. RETURNING A UNIT
14.DISPOSAL
15.TECHNICAL INFORMATION
15.1. TECHNICAL DATA
15.2. DIMENSIONAL DRAWINGS
16.EU DECLARATION OF CONFORMITY

Jan 2012 T190 Iss.2 Page 4 of 38
1. Safety
1.1. Legal considerations
It is important that you read this manual completely and observe all instructions. Failure to follow
these instructions may result in personal injury, equipment damage and incorrect performance of
the incubator.
This manual contains information for installing, operation and maintenance of the incubator. It cannot
cover all possible uses and applications. Please contact your dealer or LEEC directly if you have a
special application or the manual does not describe your problem in enough detail.
The contents of this manual and the incubator are subject to change without notice.
1.2. Intended use
Culture Safe Touch CO 2 incubators are intended for mammalian cell cultivation under typical conditions
of 37.0 °C. The pH of the cell media can be controlled by altering the CO 2 concentration within the
incubator.
Other applications are not approved by LEEC. Please contact your dealer or LEEC directly, for advice if
you intend to use this incubator for other applications.
1.3. Symbols used in this manual
The following symbols are used throughout this manual:
Risk of injury Electrical
hazard
Hot surface Lifting
hazard
Stability
hazard
Compressed
gas hazard
Asphyxiation
hazard
Mandatory
regulation
Read
operating
manual
Lift with
several
persons
Disconnect
from
electrical
supply
Important
information
for correct
operation

Jan 2012 T190 Iss.2 Page 5 of 38
1.4. General safety instructions
This incubator is intended for use by suitably qualified laboratory personnel who are familiar with all
precautions and standard safety practices for working in a laboratory environment.
These safety instructions are intended to be used alongside any local safety guidelines, rules and
regulations. If in doubt please consult the Health and Safety Officer of your organisation.
Only skilled electricians or engineers authorised by LEEC can perform repairs or maintenance on this
incubator. Only original spare parts from LEEC can be used. There are no user serviceable parts within
the incubator.
LEEC takes no responsibility for 3
rd
party accessories or equipment used with this incubator.
DANGER
Electrical hazard
Danger of death
Do not remove any panels from incubator.
Do not allow the incubator to become wet.
HOT
SURFACES
Danger of burning
Risk of injury
The glass door and inner chamber reach 200°C during sterilisation.
Do not force the door open during sterilisation.
STABILITY
HAZARD
Stability hazard
Risk of injury
Damage to unit
Do not place the unit too close to the edge of a bench.
Do not lean on the door when open.
LIFTING
HAZARD
Lifting hazard
Risk of injury
Damage to unit
Lift with 4 people
95kg
Observe safe handling regulations

Jan 2012 T190 Iss.2 Page 6 of 38
2. Description
Culture Safe Touch CO2 incubators are fitted with the latest generation of programmable controller,
ensuring precise control of temperature and CO2 levels.
Accurate and stable heating throughout the entire chamber is ensured by the unique LEEC designed
five sided direct heating element. In addition a separate independently controlled door heater is fitted to
ensure that the inner glass door is free from condensation.
The design of these element profiles promote natural convection of the air within the chamber, ensuring
even temperature and gas distribution throughout the chamber.
The Touch 190S incubator has extra heaters that allow high temperature 200°C dry sterilisation of the
incubator chamber.
2.1. Construction
The inner chamber is made from 316 grade stainless steel. It is deep drawn from one piece with no
welds and is electro-polished, meaning that the inner surfaces are smooth and easily cleaned.
The removable shelves, racking and water tray are also made from stainless steel.
The outer housing is made from stainless steel and powder coated with anti-bacterial biocide paint.
2.2. Doors
The outer door has a heater on its inner face. This door is an integral part of the temperature control
system and must be closed for the incubator to function normally.
The door is hinged on the right hand side. Left hand opening is only available as an option at the point
of ordering, retro-fitting is not possible.
When this door is open, the CO 2 valve is closed and heater power is reduced to prevent over shoot of
operating conditions. The door is linked to the controller alarm system. 

Section 6.3
On the Touch 190S incubator, 2 magnetic solenoid locks prevent the outer door from being opened
while the incubator is in a high temperature sterilise cycle.
An inner glass door seals against a thermoplastic elastomer gasket. This allows viewing of the samples
within the chamber without disturbing the temperature or atmosphere of the chamber.
The incubator can be optionally equipped with a multi-door. These multi-doors improve temperature and
CO 2 recovery after a door opening. Multi-doors can be retro fitted. 

Section 10

Jan 2012 T190 Iss.2 Page 7 of 38
2.3. Control system
The colour LCD touch screen display allows the user to easily see operating conditions and to alter
settings through icon based menus.
Software controlled PID loops constantly monitor temperature and CO2 values, making automatic
adjustments to keep the chamber atmosphere within limits.
The controller is equipped with a built in feature that logs incubator data directly to a removable SD
card. This data is in Excel format, making it easy to view with a PC. 

Section 7
A RS232 serial port is fitted as standard. This allows you to connect the incubator to a PC so that you
can remotely access the SD card data. PC software is required to do this. Contact your dealer or LEEC
directly for advice.
Other optional communication options are Ethernet PC interface and 4-20mA retransmit. 

Section 10
2.4. CO 2 Sensor
CO 2 is measured and controlled using a highly accurate infra-red sensor. This sensor remains
practically drift free between calibration periods.
The sensor is not mounted within the chamber; instead a small pump passes the chamber atmosphere
through the sensor.
The sensor does not have to be removed during high temperature sterilisation.
2.5. Diagrams
2.5.1. Outer view
Outer door
Door latch
Magnetic locks
Adjustable feet Inner glass door

Jan 2012 T190 Iss.2 Page 8 of 38
2.5.2. Inner view
Sampling port CO 2 inlet CO 2 sensor return
Chamber PT100 Shelves
Left hand racking Right hand racking
Service port CO 2 sensor intake
Inner door gasket
Water tray
2.5.3. Rear connections
Ethernet CO 2 Inlet
BMS relay Sampling port
4-20mA re-transmit
RS232 serial
Mains inlet Inlet fuse F1
Power switch

Jan 2012 T190 Iss.2 Page 9 of 38
3. Delivery, transportation and storage.
3.1. Unpacking
Inspect the packaging for signs of transportation damage.
Carefully undo the top of the cardboard packaging and gently slide the packaging over the incubator.
Inspect the incubator and inform the carrier immediately if there are any signs of transportation damage.
Remove any packaging material from within the chamber. Remove any protective coverings from the
surfaces of the inner chamber.
Remove the operating manual and accessory kit from within the chamber. Keep these safe.
Please check that the accessory pack contains the following items. Contact your dealer with details of
any shortages.
Standard T190 accessory pack
Qty
Stainless steel perforated shelves 4
Shelf racking (left and right hand) 1 set
Racking support bar 1
Stainless steel water tray 1
Service port bungs 2
Formatted 2Gb SD card 1
Operating manual 1
3.5 meter length of CO2 tubing with HEPA filter 1
Hose clips 2
Test report 1
IEC mains power lead 1
3.2. Lifting and moving.
These incubators are heavy. The weights are as follows:
Touch 190 is 94.5 Kg
Touch 190S is 104 Kg
Please refer to Manual Handling Policy of your organisation before attempting to lift the
incubators.
Risk of unit sliding or tipping.
Risk of injury by lifting heavy loads.
Risk of damage to the unit.
Always lift using the handles on the outside of the incubator.
Lift with 4 people.
Do not lift by using the door.

Jan 2012 T190 Iss.2 Page 10 of 38
3.3. Storage.
If the incubator has been in a cold location for a period then it needs to acclimatise to room
temperature.
Move the incubator to its final location and wait for 4 hours before start up. This will allow any condensation
that may have formed to dry. It will also stop the incubator from overshooting temperature set point.
4. Installation.
4.1. Location and ambient conditions
The incubator can be placed on a table or worktop. Please ensure that the site is sufficiently strong
enough to carry the weight of the incubator.
LEEC do not recommend placing the incubator directly on the floor as this increases the risk of
contamination.
Wheeled platform forms are available that lift the incubator off the floor. Alternatively two incubators can be
stacked using the stacking kit. 

Section 10
Correct location of an incubator is very important. The incubator is designed to work in a typical laboratory
environment.
For good performance the ambient conditions should be at least 6°C below the working temperature.
E.g. for a set point of 37.0°C the ambient temperature must not exceed 31.0°C.
If the difference between the set point and ambient temperatures is less than 6.0°C then the set point can
be exceeded and temperature fluctuation will occur.
Our performance data was produced at the ideal ambient condition of 25.0°C. For best performance the
ambient temperature should not drastically exceed this figure.
Maximum ambient humidity is 70% rH, non-condensing. Condensation can occur within the chamber if this
figure is exceeded.
A poorly located incubator can experience temperature fluctuations, poor cell growth and contamination of
cell cultures. These guidelines will ensure best performance:
•The incubator should be sited on a flat, even surface.
•It should be in a well ventilated and dry area, but not sited directly in the path of ventilation
ducts.
•It should be free from vibration.
•It should be kept away from heat sources e.g. radiators, heating pipes, refrigeration plant.
•It should not be in direct sunlight.
•To avoid contamination it should not be placed directly on the floor.
•Allow sufficient distances between adjacent equipment.
•There should not be big swings in ambient temperature.
Leave a clearance of 50mm from each side and 100m from the rear.
Level the incubator by adjusting the two front feet. Place a small spirit level on the centre position of the
middle shelf as a guide.
When it is level, secure the feet by tightening the lock nuts with a 10mm spanner.

Jan 2012 T190 Iss.2 Page 11 of 38
4.2. Shelves and usable space
Each incubator is supplied with 4 perforated stainless steel shelves. The shelves locate into removable
stainless steel racking. The shelves incorporate an anti-tilt mechanism, stopping the shelves from being
pulled out too far.
Each shelf measures 690mm wide by 390mm deep. The minimum spacing between shelves is 80mm.
There are 8 possible positions for the shelves.
The shelves and racking can be left in place during sterilisation.
The usable space within the incubator is the volume in which the conditions are within our published
data.
A, B, C = Internal dimensions
a = distance from left and right wall = 70mm
b = distance from top = 70mm
c = distance from front and back wall = 70mm
d = distance from base = 70mm
For practical purposes this is the perforated area on each shelf.
•Do not place any samples outside of this space.
•Do not over fill each shelf.
•Allow sufficient room between samples to allow for airflow.
•Do not use the top shelf for very deep samples.
4.3. Water tray
A stainless steel water tray is supplied which allows the incubator to provide 90%rH.
Place the tray is on the floor of the incubator, locating it with the side racking.
Carefully fill the tray with 1.5 litres of distilled, sterilised water.
LEEC recommend that you clean and refill the tray every week.
Any spillages within the chamber should be removed and cleaned immediately. Water left on the chamber
floor will cause condensation to form.
Under no circumstances should any equipment be placed in the water tray or on the chamber floor.
Remove the water tray before moving the incubator.
Empty the water tray if the incubator is switched off, otherwise condensation will occur.

Jan 2012 T190 Iss.2 Page 12 of 38
4.4. Gas connection
Risk of injury. Compressed gas.
Risk of damage to unit.
Observe relevant regulations for handling CO 2
Risk of asphyxiation. Site must have adequate ventilation.
Risk of CO 2 poisoning. Site must have adequate ventilation.
The incubator is designed for use with CO2 of 99.5% purity. Input pressure greater than 1.0 bar (15
psi) will damage the incubator so a pressure reducing valve (PRV) must be used.
The CO 2 inlet to the incubator is via a 4mm barbed push fitting.
PRV’s with the correct fittings and other gas accessories can be supplied by LEEC. 

Section 10
For correct connection of CO 2, follow these steps in order:
1) Connect the supplied tubing and HEPA filter to the gas supply.
2) Fully close the PRV.
3) Open the main valve on the gas bottle or central supply.
4) Adjust the PRV pressure of the gas supply to between 0.3 bar and 0.7 bar (4.5 to 10 psi).
5) Close the main valve on the gas bottle / central supply.
6) Connect the other end of the tubing to the incubator CO 2 inlet. Secure with the supplied clip.
Repeat these steps whenever you change the CO 2 bottle or supply.
Turn off the main valve and disconnect from the CO 2 supply if the incubator is to be unused for extended
periods.

Jan 2012 T190 Iss.2 Page 13 of 38
4.5. Electrical connection
Risk of injury.
Risk of damage to unit. Observe correct mains supply voltage.
Risk of electrical shock. Do not connect with wet hands.
No user serviceable parts. Do not remove service panels.
Disconnect unit in an emergency.
Before connecting the incubator, check that the electrical supply corresponds with the values on the
incubator data plate. If in doubt consult a qualified electrician.
The incubator is designed to operate from a single phase electrical supply of: 230V AC ±10% 50/60Hz.
This incubator MUST be connected to a protective earthed supply.
Further details can be found in the technical data. Section 15.1
Plug the supplied power lead into the IEC connector on the rear of the incubator.
Plug the other end of the IEC lead into the electrical supply.
In an emergency you may need to isolate the incubator from the electrical supply by disconnecting the
mains supply plug. Make sure that the mains supply plug is easily accessible.
5. Getting started
5.1. Start up
Insert the pre-formatted SD card (supplied) into the slot to the right of the controller.
Turn on the unit using the power switch on the rear panel.
The controller will boot up and perform a self-check.

Jan 2012 T190 Iss.2 Page 14 of 38
5.2. Setting time and date
After the controller has booted up, the time and date screen will be shown. Time and date are shown in the
format hh:mm:ss and dd/mm/yy.
If the time and date is correct press:
Proceed to step 5.3
If the time and date are incorrect press the time display. This screen will be shown:
Use the touch screen to enter the correct time.
Press to confirm selection.
Repeat these steps for setting the date.
5.3. Factory presets
The following values are preset to allow you to work straight away:
Parameter
Value
Set Point, Temperature 37.0°C
Set Point, CO 2 5.0%
High temperature alarm +1.0°C
relative to set point
Low temperature alarm -1.0°C
relative to set point
High CO 2 alarm +1.0%
relative to set point
Low CO 2 alarm -1.0%
relative to set point
Door open alarm timer 30 seconds

Jan 2012 T190 Iss.2 Page 15 of 38
5.4. Main display
The main screen shows chamber set points (SP) and process values (PV). It also shows the status of any
alarm and has touch screen buttons for accessing further functions.
Temperature process value Chamber temperature set point
CO 2 process value Chamber CO 2 set point
Sterilise cycle start Menu escape
5.5. Changing temperature set points and high / low alarms.
To change the temperature set point, touch this area of the main screen:
Touch here to alter set point
Touch here to alter high alarm limit
Touch here to alter low alarm limit
Use the key pad to change the set point.
Maximum set point is limited to 60.0 °C
Minimum set point is limited to 25 °C
Press to confirm selection.

Jan 2012 T190 Iss.2 Page 16 of 38
The high and low alarm limits are relative to the chamber set point.
For instance if the set point is 37.0 °C and the high alarm limit is + 1.0 °C then the alarm will trigger at
38.0°C.
If the set point is changed to 36.5 °C then the high alarm will trigger at 37.5°C.
Use the keypad to change the high alarm threshold.
Press to confirm selection.
Use the keypad to change the low alarm threshold.
This must be negative so remember to first press.
Press to confirm selection.
5.6. Changing CO2 set points and alarm thresholds
To change the temperature set point, touch this area of the main screen:
Touch here to alter set point
Touch here to alter high alarm limit
Touch here to alter low alarm limit

Jan 2012 T190 Iss.2 Page 17 of 38
Use the key pad to change the set point.
Maximum set point is limited to 20.0%
Minimum set point is limited to 0.0%
Press to confirm selection.
If the CO 2 set point is 0.0% then CO 2 control is disabled.
Use the keypad to change the high alarm threshold.
Press to confirm selection
Use the keypad to change the low alarm threshold.
This must be negative so remember to first press.
Press to confirm selection.

Jan 2012 T190 Iss.2 Page 18 of 38
5.7. Alarm status screen
The alarm icon gives a visual indication of alarm status.
Pressing the alarm icon opens the alarm status screen:
High temperature alarm
Low temperature alarm
High CO 2 alarm
Low CO 2 alarm
Door open alarm
SD card alarm
Low gas pressure alarm
In an alarm condition an audible buzzer sounds and the alarm icon changes to red.
Press this red alarm icon and the alarm status screen will show the corresponding alarm will in red.
For instance this shows high temperature alarm condition: Pressing this will mute the alarm.
The alarm is now acknowledged and the icons show this by changing to yellow:
The icons remain yellow for as long as the incubator is in an alarm condition.
This process is the same for all alarms.
As an additional warning the colours of the process values change during alarm condition.
The temperature process value has changed colour from black
to red.
The CO2 process value will change colour from blue to red
when it enters alarm conditions.

Jan 2012 T190 Iss.2 Page 19 of 38
6. Other features
6.1. Graphical display
The controller has a built in feature that allows the user to view graphs of both temperature and CO 2. It
stores a minimum of 3 days of graphical data. When the internal memory is full it will over-write the oldest
data first.
Graphs are only stored while the unit is powered.
This feature is independent of the SD data logging function. 

Section 7
On the main screen press the graph symbol:
The graph menu opens:
Choose which graph you wish to view
In this instance we are viewing the temperature graph:
Press the y-axis limits.
The values can be changed using the keypad.
Press Use to view the temperature stability against time.
To return to the Main Screen press the escape button
The CO 2 graph is viewed in the same manner.

Jan 2012 T190 Iss.2 Page 20 of 38
6.2. Utility Menu
On the main screen press the utility menu symbol:
The utility menu combines all of the incubator settings menus in one place. You will already be familiar with
some of these.
6.3. Door alarm menu
Press the door icon:
The door has a magnetic switch that senses when the door
is opened.
The door icon turns red as soon as the door is opened. After
a set time the alarm will sound. This can be muted in the
alarm status menu. Section 5.7
This delay can be changed by pressing here.
The format is mm:ss
Clock menu. 


Section 5.2
Temperature menu. 


Section 5.5
CO 2 menu. 


Section 5.6
User security menu. 


Section 6.
6
SD card menu. 


Section
7
Door alarm menu. 


Section 6.3
Alarm status menu. 


Section 5.7
Historical event log. 


Section 6.5
Incubator information. 


Section 6.4
User log out. 


Section 6.
6