Leica ASP300 User manual

Instruction manual
Leica ASP300
V1.3 English - 12/2003
Always keep this manual togethe with the inst ument.
Read this inst uction manual ca efully
befo e wo king with the inst ument.
Leica ASP300
Advanced Smart Processor
Automated vacuum
tissue processor

3
Leica ASP300
NOTE
The info mation, nume ical data, notes and
value judgments contained in this manual ep-
esent the cu ent state of scientific knowl-
edge and state-of-the-a t technology.
We a e unde no obligation to update the
p esent manual acco ding to the latest tech-
nical developments, no to p ovide ou cus-
tome s with additional copies, updates etc. of
this manual.
Fo e oneous statements, d awings, techni-
cal illust ations etc. contained in this manual
we exclude liability as fa as pe missible ac-
co ding to the national legal system applicable
in each individual case. In pa ticula , no liabili-
ty whatsoeve is accepted fo any financial
loss o consequential damage caused by o
elated to compliance with statements o othe
info mation in this manual.
Statements, d awings, illust ations and othe
info mation as ega ds contents o technical
details of the p esent manual a e not to be
conside ed as wa anted cha acte istics of
ou p oducts.
These a e dete mined only by the cont act
p ovisions ag eed between ou selves and ou
custome s.
Leica ese ves the ight to change technical
specifications as well as manufactu ing p o-
cesses without p io notice. Only in this way is
it possible to continuously imp ove the tech-
nology and manufactu ing techniques used in
ou p oducts.
This document is p otected unde copy ight
laws. Any copy ights of this document a e e-
tained by Leica Mic osystems Nussloch
GmbH.
Any ep oduction of text and illust ations (o of
any pa ts the eof) by means of p int, photo-
copy, mic ofiche, web cam o othe methods –
including any elect onic systems and media –
equi es exp ess p io pe mission in w iting by
Leica Mic osystems Nussloch GmbH.
© Leica Mic osystems Nussloch GmbH
Published by:
Leica Mic osystems Nussloch GmbH
Heidelbe ge St . 17 - 19
D-69226 Nussloch
Ge many
Telephone: [phone]
Fax: [phone]
Inte net: http://www.histo-solutions.com

4Instruction manual V 1.3 – 12/2003
Table of contents
1. Important notes............................................................................................................................. 6
1.1 Symbols used in this manual................................................................................................... 6
1.2 Designated use .......................................................................................................................... 6
2. Safety .............................................................................................................................................. 7
2.1 Safety inst uctions..................................................................................................................... 7
2.2 Wa nings...................................................................................................................................... 8
2.3 Integ ated safety devices...................................................................................................... 10
3. Instrument components and specifications .......................................................................... 11
3.1 Specific inst ument options................................................................................................... 11
3.2 Ove view - inst ument components .................................................................................... 12
3.3 Standa d delive y - packing list............................................................................................ 14
3.4 Technical data ......................................................................................................................... 15
3.5 Compatible eagents............................................................................................................... 18
4. Setting up the instrument .......................................................................................................... 19
4.1 Site equi ements..................................................................................................................... 19
4.2 Elect ical connection.............................................................................................................. 19
4.3 Installing the accesso ies ..................................................................................................... 21
4.4 Setting up the data connections.......................................................................................... 22
4.5 Ala m functions ........................................................................................................................ 23
4.6 Switching the inst ument on ................................................................................................. 24
4.7 Touch sc een functions......................................................................................................... 26
4.7.1 Use inte face - ove view ...................................................................................................... 26
4.7.2. Access cont ol ......................................................................................................................... 27
4.8 Check list fo fi st ope ation (at supe viso access level).............................................. 28
5. Operation...................................................................................................................................... 29
5.1 Reagent handling..................................................................................................................... 29
5.1.1 Fill/d ain eagents (othe than wax) .................................................................................... 29
5.1.2 Changing the wax .................................................................................................................... 31
5.2 Setting up the inst ument pa amete s................................................................................ 34
5.2.1 System setup............................................................................................................................. 34
5.2.2 Editing the eagent list ............................................................................................................ 37
5.2.3 Viewing the p og am list......................................................................................................... 40
5.2.4 Adding and/o modifying p og ams .................................................................................... 41
5.2.5 Favo ites (maximum of 5 p og ams) .................................................................................... 44

5
Leica ASP300
Table of contents
5.2.6 Editing the stations .................................................................................................................. 46
5.2.7 Reagent g oups........................................................................................................................ 47
5.3 P og ams.................................................................................................................................... 48
5.3.1 Editing a selected p og am ................................................................................................... 49
5.3.2 Sta ting a p og am................................................................................................................... 51
5.4 Reagent status ......................................................................................................................... 52
5.5 System Monito ......................................................................................................................... 53
5.6 On-line help ............................................................................................................................... 54
5.7 Configu ing a p inte (optional)............................................................................................. 55
6. Trou le shooting ........................................................................................................................ 56
6.1 Gene al ....................................................................................................................................... 56
6.2 Powe failu e............................................................................................................................. 56
6.3 T ouble shooting...................................................................................................................... 57
6.4 Typical fill o d ain p oblems ................................................................................................. 58
7. Cleaning and maintenance ......................................................................................................59
7.1 Clean p og ams ........................................................................................................................ 59
7.1.1 Reto t clean p og ams............................................................................................................ 59
7.1.2 Wax clean p og ams .............................................................................................................. 61
7.1.3 Sma t clean ............................................................................................................................... 62
7.2 Gene al cleaning steps .......................................................................................................... 63
7.3.1 Daily cleaning and maintenance ......................................................................................... 65
7.3.2 Pe iodic cleaning and maintenance................................................................................... 66
7.4 P eventative maintenance schedule ................................................................................. 67
8. Warranty and service ................................................................................................................ 69

6Instruction Manual V 1.3 – 12/2003
1.1 Sym ols used in this manual
Warnings
appear in a grey box and are marked
by a warning triangle .
Notes,
i.e. important user information appear
in a grey box and are marked by an
information symbol .
Flammable solvents and reagents are
marked with this symbol.
Instrument surfaces which become
hot during operation are marked with
this symbol.
Figures in brackets refer to item nos.
in drawings.
Function keys to be pressed on the
instrument touch screen are written
in bold-print capital letters.
1. Important notes
1.2 Designated use
The Leica ASP300 is an automated tissue p o-
cesso fo the following labo ato y applications:
• fixation
• dehyd ation
• pa affin wax infilt ation
of histological tissue specimens.
The Leica ASP300 must be ope ated exclu-
sively with the eagents listed in
Chapte 3.5 - “Compatible eagents“.
Any other use of the instrument
is considered improper!
(5)
ENTER
and their meaning

7
Leica ASP300
2. Safety
2.1 Safety instructions
This inst uction manual includes impo tant in-
fo mation elated to the ope ating safety and
maintenance of the inst ument and it is an im-
po tant pa t of the p oduct.
If additional re uirements on accident
prevention and environmental protec-
tion exist in the country of operation,
this instruction manual must be
supplemented by appropriate instruc-
tions to ensure compliance with such
re uirements.
Instrument type
All info mation p ovided in this manual applies
only to the inst ument type indicated on the title
page.
A name plate indicating the inst ument se ial
numbe is attached to the back of the inst u-
ment.
This inst ument has been built and tested in
acco dance with the following safety egula-
tions on elect ical measu ing, cont ol, egulat-
ing and labo ato y devices:
• DIN EN ISO 9001
• EN 61010-1
• EN 61010-2
• EN 61326-1 and EN 61326-2
• EN 61000-3-2 and EN 6100-3-3.
The protective devices on both in-
strument and accessories may neither
be removed nor modified.
Only authorized and ualified service
personnel may repair the instrument
and access the instrument’s internal
components.
Make sure to comply with the safety instructions and warnings in this chapter.
Make sure to read these instructions, even if you are already familiar with the operation and
use of other Leica products.

8Instruction manual V 1.3 – 12/2003
2. Safety
2.2 Warnings
The safety devices installed in this inst ument by the manufactu e only constitute the basis
fo accident p evention.
P ima ily esponsible fo accident-f ee ope ation is above all the institution which owns the
inst ument and, in addition, the designated pe sonnel who ope ates, se vices o epai s the
inst ument.
To ensu e t ouble-f ee ope ation of the inst ument, make su e to comply with the following
inst uctions and wa nings.
Transport and installation
Wa nings and inst uctions on t anspo t and installation of the inst ument can be found on a
sepa ate info mation sheet attached to the outside of the inst ument’s shipping c ate.
Warnings - reagent handling
To avoid damage to the instrument, only use the reagents listed in chapter 3.5!
When handling reagents, follow the instructions of the manufacturer and make sure all
laboratory regulations in force in your country are complied with.
Dispose of waste solvents with care according to local regulations and the waste manage-
ment policy of the company or institution.
Use caution when handling hot, molten paraffin.
Do not touch paraffin stations and retort walls – they can be very hot.
Do not clean the reagent bottles in an automatic dishwasher.
Material safety data sheets can be obtained from the supplier of the chemicals.
They are also available on the Internet:
http://www.msdsonline.com

9
Leica ASP300
Warnings - working with the instrument
The Leica ASP300 may only be operated by trained laboratory personnel and as per the
present instruction manual.
In case of emergency switch off mains (item no. 5 in Fig. 3) at the rear of the instrument and
unplug the power cord.
The instrument must be installed so that the mains switch at the rear of the instrument can
easily be accessed at any time.
Always press PAUSE before opening the retort lid during processing to allow the retort to
vent.
Do not remove the wax drain hose or the remote fill/drain hose until the fill or drain has been
entirely completed.
After refilling / replacing the reagent bottles ensure that the lids are tight.
The bottles must be properly pushed home into the connection manifolds at the rear inner
wall of the reagent module.
Fixatives containing mercuric salts, acetic or picric acid will corrode metallic components
in the instrument.
After each paraffin step a retort clean cycle must be run.
2. Safety
Warnings - cleaning and maintenance
Prior to each maintenance switch the instrument off and disconnect mains power.
Do not clean the instrument with solvents containing acetone or xylene. No li uid may be
spilled into the internal components of the instrument.
Empty the condensate bottle at regular intervals.

10 Instruction manual V 1.3 – 12/2003
2.3 Integrated safety devices
The inst ument is equipped with the following p otection featu es:
• ove p essu e p otection,
• p otection against excessive vacuum,
• ove cu ent p otection,
• ove heating p otection.
Overpressure protection
The design of the p essu e system ensu es that no dange ous levels of
p essu e can build up.
Protection against excessive vacuum
The vacuum system is not capable of gene ating a dange ous vacuum
condition.
Overcurrent protection
Ove cu ent conditions a e p otected against by both the mains fuse
and the sepa ate heating powe fuses.
Overheating protection
The design of the heating system ensu es that no dange ous ove heat-
ing conditions can occu .
2. Safety

11
Leica ASP300
3. Instrument components and specifications
3.1 Specific instrument options
•Reagent anagement System (RMS).
• The specimen cassette baskets may be used with o without cas-
sette divide s and sp ing sepa ato s designed to sepa ate cassettes
and imp ove fluid penet ation.
• In-p ocess fluid eci culation (‘tidal agitation’) fo continuous and ef-
ficient mixing of fluids.
• Remote fill/d ain system fo eagents.
• Remote fill/d ain system fo pa affin.
• Automatic optical fluid level senso .
• Active pa affin wax solvent emoval featu e.
• Magnetic sti e .
• P og ammable p og am sta t time delay.
• P og ammable finish time fo p ocessing p og ams.
• 3-step eto t d ain featu e.
• Specimen p ocessing unde p essu e, vacuum, alte nating p essu e
/ vacuum cycles o unde ambient p essu e.
• Use -p og ammable clean cycles.

12 Instruction manual V 1.3 – 12/2003
3.2 Overview - instrument components
3. Instrument components and specifications
2
34
5
13
6
7
14 15
16
17 18
19
20
21
22
12 11 8
10 9
1
Fig. 1
23

13
Leica ASP300
Overview - Instrument components
1 - Basic instrument - processor module
2 - Basic instrument - reagent module
3 - Wax bath lids
4 - Retort lid
5 - Touch screen
6 - Cover flap of instrument console
7 - Instrument console comprising:
8 - Printer port
9 - Local alarm connection
10 - Remote alarm connection
11 - Floppy disk drive
12 - Serial port
13 - Activated carbon filter
14 - Condensate bottle
15 - Reagent bottle (13 units)
16 - Drip tray
17 - Wax drain connection
18 - Remote drain connection
19 - Retort
20 - Wax baths
21 - Specimen basket
22 - Lid for specimen basket
23 - Splash protector
3. Instrument components and specifications
Instrument components and accessories
The p ocesso module comp ises th ee pa af-
fin wax baths and the p ocessing eto t.
Th ee baskets (21) contain the cassettes.
Each basket can hold up to 300 cassettes.
All p ocessing occu s in the stainless steel e-
to t unde the p essu e, vacuum and tempe a-
tu e conditions selected.
The eagent bottles a e sto ed in the eagent
cabinet.

14 Instruction manual V 1.3 – 12/2003
3. Instrument components and specifications
3.3 Standard delivery - packing list
Leica ASP300 basic standa d delive y:
1 Leica ASP300 - Basic inst ument 0476 34100
1 Accesso y kit consisting of: 0476 35929
- 1 Basket hook
0476 34713
- 3 Specimen baskets, assy., including lid, divide s, sp ings and handle 0476 34193
(accesso ies fo maximum and optimum usage of basket space)
- 1 Reagent bottle, plastic 0476 34274
- 2 Activated ca bon filte s, assy. 0476 34150
- 1 Remote fill/d ain hose, assy. with special connecting device
fo emote filling / d aining of p ocessing eto t 0476 34716
- 1 Pa affin d ain hose 0476 34721
- 1 Pa affin wax sc ape , plastic 0476 35923
- 1 Lub icant ‘Molykote 111’, fo valves and O- ings, 100 g 0336 35460
- 1 Jumpe cable - mains 0411 34604
- 1 Maintenance kit (2 spa e lids, 9 O- ings) 0476 35921
- 1 Sti e ba , assy. 0476 34202
- 2 Sets of labels fo eagent bottles, each set containing 15 labels 0476 35792
- 1 Splash p otecto 0476 34770
1 Set of powe co ds:
- 1 Powe co d D 0411 13558
- 1 Powe co d UK ST-BU F-5A 0411 27822
13 Reagent bottles, plastic (included in the inst ument) 0476 34274
1 Condensate bottle, plastic (included in the inst ument) 0476 34278
1 Se vice kit fo pump (included in the inst ument) 0476 35922
1 D ip t ay (included in the inst ument) 0476 34145
1 Cove sheet fo ove flow (included in the inst ument) 0476 34852
1 Console cove (mounted to the inst ument) 0476 34162
1 3,5 Diskette, empty (taped to the back of console cove )
1 Inst uction manual Leica ASP300 multilingual 0704 37102
1 Demo-P og am, CD-Rom

15
Leica ASP300
3.4 Technical data
Nominal voltage: Two facto y-p eset voltage settings
(not use -adjustable):
100 to 120 V + 10% o
230 to 240 V + 10%
Nominal f equency: 50 to 60 Hz
Main fuses: 2 fast-blowing melting fuses, 20 x 5 mm, UL-listed
O de no.
• 100 to 120 V; F 10 A 250 VAC 69411002
• 230 to 240 V; F 5 A 250 VAC 69411000
Nominal powe : 1,000 VA
Dimensions (W x D x H), in mm: 595 x 680 x 1,325
D y weight, unpacked: app ox. 140 kg
Weight, packed: 220 kg
Ope ating tempe atu e ange: 15 °C to 35 °C
Relative humidity: 10% to 80% - non-condensing
IEC 1010 classification: P otective Class 1
Pollution Deg ee 2
Ove voltage Installation Catego y II:
• 800 V impulse (120 V systems)
• 1500 V impulse (240 V systems)
Maximum ope ating altitude: maximum 2,500 m
Local / emote ala m elays: 30 V DC, maximum 2 A
2 connections:
both single voltage-f ee, change-ove contacts
(can be ope ated as both no mally-open and
no mally-closed connections)
Paraffin wax baths
Numbe of baths: 3
Volume (lite s): 4.3 l pe bath
Melt time: app ox. 10 h s
Tempe atu e: 40 to 65 °C
Tempe atu e accu acy: + 1 °C
3. Instrument components and specifications

16 Instruction manual V 1.3 – 12/2003
Retort
Capacity: max. 300 cassettes
Reagent volume (in lite s): 4.3
Tempe atu e (pa affin): 40 to 65 °C
Tempe atu e (p ocessing eagents): ambient o 35 to 55 oC
Tempe atu e (cleaning eagents): 50 to 65 °C
Tempe atu e accu acy: + 1 K
Filling time: app ox. 90 sec
D ain time : app ox. 80, 120, 140 sec (adjustable)
Imp egnation vacuum: -70 kPa (g)
Imp egnation p essu e: 35 kPa (g)
Fill vacuum: -70 kPa (g)
D ain p essu e: 35 kPa (g)
General
Reagent bottles 10
Cleaning solution bottles: 3
Maximum bottle volume: 4.3 l
P inte : Optional accesso y
P etest check: ON/OFF
Fluid level senso : ON/OFF
Reci culation (pump in/out): ON/OFF
(a) Time befo e 1st cycle: 12 min
(b) Time between cycles: 20 min
System setup
Passwo d status: Supe viso / Use
Type of passwo d: alphanume ic, f eely selectable
Reagent Management Status: ON/OFF
Softwa e access cont ol (‘Lock Mode’): ON/OFF
3. Instrument components and specifications

17
Leica ASP300
3. Instrument components and specifications
3.4 Technical Data (continued)
Hardware and Software:
• La ge colo liquid c ystal display (LCD) and touchsc een.
• Use -f iendly, intelligent softwa e.
• 3,5“ floppy d ive and p inte po t.
• Ala m system with two emote ala m sockets.
• Passwo d-p otected inst ument supe viso mode.
• Multiple specimen p otection system.
Capacities:
• 15 p og ams of up to 10 eagent and 3 pa affin wax steps each.
• Time pe p og am step: 0 to 99 hou s , 59 minutes.
• Delay time: max. 7 days.
• Up to 300 cassettes can be p ocessed simultaneously.
• Fou use -p og ammable eto t clean p og ams.
• Wax clean p og am.
• 10 Reagent bottles.
• 3 Wax baths.
• 3 Cleaning solution bottles.
• 1 Condensate bottle.
• Reagent tempe atu e adjustable f om 35°C to 55°C
o ambient.
• Pa affin tempe atu e adjustable f om 40°C to 65°C.
• Choice of th ee eto t d ain ates of 80, 120 and 140 seconds.
• Up to 100 eagent names in memo y.

18 Instruction manual V 1.3 – 12/2003
3.5 Compati le reagents
Fixatives
1. Fo malin, buffe ed o unbuffe ed
2. Fo malin saline
Dehydration
1. Ethanol 4. Butanol
2. Isop opanol 5. Indust ial Methylated Spi its
3. Methanol
Clearing
1. Xylen ( ecommended)
2. Toluol *
3. Chlo ofo m *
Paraffin wax
1. Pa affin
Caution:
Reagents other than those listed here may damage some com-
ponents of the instrument.
Do not use acetone, benzene or trichlorethane in the instru-
ment.
Fixatives containing mercuric salts, acetic or picric acid ill
corrode metallic components in the instrument and shorten in-
strument life.
If you choose to ork ith such fixatives, it is essential to per-
form a clean cycle hich contains multiple ater rinses each
time after use, to minimize damage.
In addition, e recommend frequent and regular preventive
maintenance by the Leica Technical Service.
3. Instrument components and specifications
* P io to using these eagents, contact
you Leica ep esentative o Leica di-
ectly fo info mation on necessa y p o-
tective measu es. Using these eagents
will equi e an inc eased numbe of main-
tenance inte ventions by Leica Techni-
cal Se vice.

19
Leica ASP300
Moving the instrument
The instrument must be installed such that the mains s itch at
the rear of the instrument (item no. 5 in Fig. 3) can easily be ac-
cessed at any time.
Afte the inst ument has been unpacked (see unpacking inst uctions
attached to outside of t anspo t c ate) it must be touched only at the
points ma ked with dots ‘ ‘ fo t anspo t to its final site of installation.
Once the inst ument has been installed the e, the b akes on the inst u-
ment olle s must be locked.
4.1 Site requirements
• The inst ument needs app ox. 650 x 700 mm floo space.
• P actically vib ation-f ee floo .
• Room tempe atu e constantly between +15 °C and +35 °C.
• Relative humidity maximum 80%, non-condensing.
• Avoid vib ations, di ect sunlight and heavy va iation in tempe atu e.
4. Setting up the instrument
Fig. 2
4.2 Electrical connection
• Check the voltage label (Fig. 2) on the ea of the inst ument to ensu e
that the inst ument is set to the co ect voltage ange.
The chemicals to be used in the Leica ASP300 are both flam-
mable and noxious. Therefore, the instrument must be set up in
a ell ventilated area, free from any ignition sources.
The room here the instrument is installed is not suitable for
people to stay in for longer periods of time, unless it is
equipped ith a fume extraction system.
Do not operate the instrument in rooms ith explosion hazard.
The po er supply voltage for the instrument is factory-preset
and CANNOT be altered by the user.

20 Instruction manual V 1.3 – 12/2003
4. Setting up the instrument
• Connect mains cable (6) to mains powe supply socket (1) (see Fig. 3).
• Jumpe cable (3) connects the mains powe supply output (2) to the
elect onics module input (4).
• Switch ON/OFF switch (5) ON.
• Switch ON/STOP switch at the ight of the inst ument ON.
Fig. 3
1
2
6
3
4
5
Electrical
connections at the
rear panel of the
instrument
Cables
connected
correctly
Important:!
Specification for connection (2) is as follo s:
100 - 120 V or 230 - 240 V, max. 200VA.
Connecting the instrument to mains
The instrument MUST be connected to an earthed mains power
outlet socket.
It is recommended that the ASP300 be plugged into a wall socket
that has Ground Fault Circuit Interruption (GFCI) protection - as
an additional electrical safeguard.
Make sure to use the appropriate mains cable for the local volt-
age supply (wall outlet).