Leica S8 APO B User manual

Leica S Series User Manual 1
Living up to Life
Leica S8 APO B
User Manual

Leica S Series User Manual 2
Copyright
Revision 1.0, published March 14, 2013 by:
Leica Microsystems Wetzlar GmbH
Ernst Leitz-Straße 17-37
D-35578 Wetzlar (Germany)
http://www.leica-microsystems.com
Responsible for contents:
Marketing CMS
All rights to this documentation are held by
Leica Microsystems CMS GmbH. Reproduction
of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method
(including electronic systems) is not allowed
without express written permission from Leica
Microsystems CMS GmbH.
The instructions contained in the following
documentation reflect state-of-the-art tech-
nology. We have compiled the texts and illus-
trations as accurately as possible. Still, we are
always grateful for comments and suggestions
regarding potential mistakes within this docu-
mentation.
The information included in this manual may
be changed without prior notice.

Leica S Series User Manual 3
Function of the Microscopes
The Leica S8 APO B microscope, for which
this User Manual has been written, is used for
routine examinations of cell and tissue cultures,
liquids and sediments. This includes examining
specimens taken from the human body for the
purpose of gaining information about physi-
ological or pathological conditions or inborn
anomalies, or testing for safety and compat-
ibility for potential recipients, or for monitoring
therapeutic measures.
IVD
The above-named microscopes comply with
the Council Directive 98/79/EC concerning in
vitro diagnostics.
The manufacturer assumes no liabil-
ity for damage caused by, or any
risks arising from, using the microscope for
other purposes than those for which they are
intended or not using them within the speci-
fications of Leica Microsystems CMS GmbH.
In such cases, the Declaration of Conformity
shall be invalid.
Leica S8 APO B identification label
These (IVD) instruments are not
intended for use in the patient environ-
ment defined by DIN VDE 0100-710. Nor are
they designed to be combined with medical
instruments in accordance with EN 60601-1. If a
microscopeiselectricallyconnectedtoamedical
instrument in accordance with EN 60601-1, the
requirements listed in EN 60601-1-1 shall apply.
Not suitable for examining potentially infec-
tious specimens. This type of instrument may
be operated by trained laboratory personnel
only.

Leica S Series User Manual 4
General Notes
Use in clean rooms
The Leica S series can be used in clean rooms
without any problems.
Cleaning
▶Do not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
▶Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces,
and products could be contaminated by
abraded particles.
▶In most cases, we can provide special solu-
tions on request. Some products can be
modified, and we can offer other accesso-
ries for use in clean rooms.
▶The cleaning of glass surfaces and objec-
tives in particular should be carried out
exclusively as outlined in the brochure
"Cleaning of Microscope Optics". The infor-
mation can be downloaded at:
http://www.leica-microsystems.com/prod-
ucts/
. Select your product and go to the "Down-
load" page.
▶For additional information, refer to page
51.
Servicing
▶Repairs may only be carried out by Leica
Microsystems-trained service technicians.
Only original Leica Microsystems spare
parts may be used.
Responsibilities of person in charge of
instrument
▶Ensure that the Leica stereomicroscope
is operated, maintained and repaired by
authorized and trained personnel only.

Leica S Series User Manual 5
Important Safety Notes
User Manual
This User Manual describes the special func-
tions of the Leica StereoZoom® stereomicro-
scopes (S series) and contains important
instructions for their operational safety, main-
tenance, and accessories.
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the User Manual
and the safety instructions from the supplier.
Before installing, operating or using the instru-
ments, read the user manuals listed above. In
particular, please follow all safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
We guarantee the quality of our products.
Our guarantee covers all faults in materials
and manufacture. It does not, however, cover
damage resulting from careless or improper
handling.

Leica S Series User Manual 6
Symbols Used
Warning! Safety hazard!
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
▶Hazards to personnel
▶Functional disturbances or damaged
instruments
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
▶Hazards to personnel
▶Functional disturbances or damaged
instruments
Danger due to hot surface.
This symbol warns against touching hot
surfaces, e.g. those of light bulbs.
Important information
This symbol indicates additional infor-
mation or explanations that are intended
to provide clarity.
Explanatory notes
▶This symbol within the text stands for addi-
tional information and explanations.
Figures
(1) Numbers in parentheses within the
descriptions relate to the figures and the
items within those figures.
Disposal.
Notes on how to dispose of the micro-
scope, its components and consum-
ables.
China RoHS 50 year EFUP (Environ-
mentally friendly use period)
IVD labeling
IVD Instrument for in vitro diagnostics.
MM/YYYY IVD manufacturing date,
for example 11 / 2011 for
November 2011.

Leica S Series User Manual 7
Safety Instructions
Description
▶The individual modules fulfill the highest
requirements for observation and docu-
mentation of Leica stereomicroscopes of
the M series.
Intended use of instrument
▶Leica Microsystems microscopes are opti-
cal instruments for improving the visibility
of objects or specimens through magnifi-
cation. Accessories such as optical acces-
sories, stands, illumination, cameras etc.
supplement the equipment configuration.
Non-intended use
▶Using the instrument in any way contrary
to the specifications in the User Manual can
lead to bodily harm and damage to objects.
Never use microscopes for in vivo examina-
tions or eye surgery if they are not expressly
intended for such use. Never install any
other plug or unscrew optical systems
and mechanical parts unless expressly
instructed to do so in the instructions.
The instruments and accessories described in
this User Manual have been tested for safety and
potential hazards. The responsible Leica affiliate
must be consulted whenever the instrument is
altered, modified or used in conjunction with
non-Leica components that are outside of the
scope of this manual!
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims as well as product liability and
the Declaration of Conformity.
Place of use
▶Only use the instruments in enclosed,
dust-free rooms and between +10 °C and
+40 °C. Protect the devices from oil, chemi-
cals and extreme humidity. If using the
devices outdoors, protect them from dust
and moisture. Never use electrical devices
outdoors.
▶Electrical components must be placed at
least 10 cm away from the wall and away
from flammable substances.
▶Avoid large temperature fluctuations,
direct sunlight and vibrations. These condi-
tions can distort micrographic images, for
example.
▶In warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the build-up of
fungus.

Leica S Series User Manual 8
Safety Instructions (continued)
Responsibilities of person in charge of
instrument
▶These Safety Instructions must be available
at the workplace.
Ensure that:
▶The M series stereomicroscopes and
accessories are operated, maintained and
repaired by authorized and trained person-
nel only.
▶All operators have read, understood and
observe this User Manual, and particularly
the safety regulations.
Repairs, service work
▶Repairs may only be carried out by Leica
Microsystems-trained service technicians.
▶Only original Leica Microsystems spare
parts may be used.
▶Before opening the instruments, switch off
the power and unplug the power cable.
▶Avoid contact with powered electrical
circuits, which can lead to injury.
Transport
▶Use the original packaging for shipping or
transporting the individual modules of the
Leica M stereomicroscopy series and the
accessory components.
▶In order to prevent damage from vibrations,
disassemble all moving parts that (accord-
ing to the user manual) can be assembled
and disassembled by the customer and
pack them separately.
Integration in third-party products
▶When installing Leica products into third-
party products, the manufacturer of the
complete system or its dealer is responsible
for following all applicable safety instruc-
tions, laws and guidelines.
Disposal
▶Once the product has reached the end of its
service life, please contact Leica Service or
Sales about disposal.
▶Please observe and comply with the
national and federal laws and regulations
that are equivalent to EC directives such as
WEEE.
Like all electronic devices, the micro-
scope, its accessory components and
consumables must never be disposed of with
general household waste.

Leica S Series User Manual 9
Safety Instructions (continued)
Legal regulations
▶Observe the generally applicable statu-
tory and country-specific regulations for
accident prevention and environmental
protection.
EC Declaration of Conformity
▶Electrically operated accessories are
constructed based on the state of the art
of technology and are provided with an EC
Declaration of Conformity. See page 50.
Health risks
Workplaces with stereomicroscopes facilitate
and improve the viewing task, but they also
impose high demands on the eyes and holding
muscles of the user. Depending on the dura-
tion of uninterrupted work, asthenopia and
musculoskeletal problems may occur. For this
reason, appropriate measures for reduction of
the workload must be taken:
Optimal arrangement of workplace, work
assignments and work flow (changing tasks
frequently). Thorough training of the person-
nel, giving consideration to ergonomic and
organizational aspects.
The ergonomic design and construction of the
Leica M stereomicroscopy series are intended to
reduce the exertion of the user to a minimum.
Direct contact with eyepieces can be a potential
transmission path for bacterial and viral infec-
tions of the eye.
The risk can be kept to a minimum by using
personal eyepieces for each individual or
detachable eyecups.

Leica S Series User Manual 10
Safety Instructions (continued)
Light sources: safety regulations
▶Light sources pose a potential irradia-
tion risk (glare, UV radiation, IR radiation).
Therefore, lamps have to be operated in
closed housings and in installed condition.
▶Never look directly into the beam path
(blinding hazard).
▶Do not select a white, strongly reflective
background for the specimen.
Careful handling
▶Exercise particular care when setting up
the instruments. If it is specified that two or
more people are required for setup, compli-
ance with this is mandatory.
▶Never spill any liquids on electrical instru-
ments. This could cause the stereomicro-
scope and other equipment to become
electrically live and damage people and
instruments.
▶Never clean instruments using corro-
sive cleaning agents or those containing
acetone. For detailed information about
care, refer to the User Manual for the instru-
ment.
▶Check the power cables regularly. Defective
power cables can cause injuries.
▶Wait for bulbs to cool off before changing
them. Touching hot bulbs can cause burns.

Leica S Series User Manual 11
Copyright 2
Function of the Microscopes 3
General Notes 4
Important Safety Notes 5
Symbols Used 6
Safety Instructions 7
Contents 11
Leica S8 APO B
Congratulations! 14
What Your Stereomicroscope Offers You 15
On We Go 16
Assembly
TL ST Transmitted Light Base 18
Optics Carrier and Additional Objective 19
Available Graticules 20
Inserting the Graticules 21
Eyepieces 22
Mounting a Camera 23
Quick Start Guide
Overview of the Leica S8 APO B 25
Tips for Working Ergonomically 26
Using the Eyepieces 27
The Correct Interpupillary Distance 28
Focusing 29
Changing Magnification (Zooming) 30
Limiting Zoom Range 31
Limiting Zoom Range (continued) 32
Regulating the resistance of the focusing drive 33
Changing the position of the optics carrier 34
Dioptric Correction with Two Adjustable Eyepieces 35
Leica TL ST Transmitted Light Base: Controls 38
Leica TL ST Transmitted Light Base: Operation 39
Leica TL ST Transmitted Light Base: Lamp Replacement 40
Photography with the Leica S8 APO B 41
Dimensional Drawings in mm
Leica S8 APO B with Transmitted Light Illumination 43
Contents

Leica S Series User Manual 12
Technical Data
Technical Data 45
Leica TL ST Transmitted Light Base 46
Appendix
Calculating the Total Magnification/Field of View Diameter 48
Troubleshooting 49
Care, Maintenance, Contact Persons 50

Leica S Series User Manual 13
Leica S8 APO B

Leica S Series User Manual 14
Congratulations!
Congratulations on obtaining your new Leica StereoZoom® line stereo-
microscope (S series). We are convinced that it will more than meet your
expectations. This instrument embodies all the qualities you associate
with the name Leica Microsystems: excellent objectives, high-quality
engineering and reliability. Furthermore, the modular design ensures that
the Leica stereomicroscope adapts perfectly to your needs – no matter
which accessories you require for your tasks.
Thanks to the parfocal system, you can always obtain a precise, sharp
display of your microscopic specimens at both large working distances
and large fields of view – from the complete image down to the finest
enlarged detail.
Though the reliability and robustness of Leica stereomicroscopes is world-
renowned, like any high-tech product, the Leica S series requires a certain
degree of care and attention. Therefore, we recommend that you read
this manual. It contains all the information you need regarding operation,
safety and maintenance. Simply observing a few guidelines will ensure
that even after years of intensive use, your stereomicroscope will continue
to work as smoothly and reliably as on the very first day.
We wish you the best of success in your work— after all, you are now
equipped with the best tool!

Leica S Series User Manual 15
What Your Stereomicroscope Offers You
The optical system of the Leica StereoZoom®
line consists of two beam paths converging at
12°. The objective pairs of each optical path are
positioned close together, so the stereomicro-
scopes can be of very "slender" design, espe-
cially towards the base of the instrument. The
advantages of this design are that it has a small
space requirement for use on bonders and in
machine applications, unobstructed access
to specimens, plenty of space for tools and a
completely clear view of the object field.
The Greenough system enables cost-effective
correction of aberrations such as chromasia,
image field curvature, and distortion with mini-
mal effort. In the new Leica StereoZoom® line,
the optimum corrected center of the objec-
tive is used for the image. This provides supe-
rior optical performance with large, level and
undistorted fields of view and chromatically
optimized, high-contrast images.
Photography
The StereoZoom® Leica S8 APO B model is
equipped with an integrated video/phototube,
allowing for easy, quick digital camera assem-
bly (Leica MC170 HD).
Apochromatic correction
The Leica S8 APO B is a completely apochro-
matically corrected Greenough system. The
apochromatic optics correct chromatic aberra-
tion perfectly, remove bothersome color seams
and display pin-sharp images of even the finest
enlarged detail. The contrast, brilliance, sharp-
ness, resolution, color fidelity and image preci-
sion are unsurpassed. The benefit of apochro-
matic correction is best seen in specimens that
have a fine, low-contrast structure such as large
animal cells, cilia plants or metallic microelec-
tronic structures.
The technical features are found on page 44.

Leica S Series User Manual 16
On We Go
If your new Leica stereomicroscope has already
been assembled and commissioned by your
Leica consultant, click here to skip through the
installation instructions and go directly to the
Quick Start Guide on page 24.
If, on the other hand, you are assembling the
stereomicroscope yourself, continue with the
"Assembly" chapter, which begins on page
17.

Leica S Series User Manual 17
Assembly

Leica S Series User Manual 18
TL ST Transmitted Light Base
Unpacking the base
The base is delivered with the adapter plate
installed. Make sure the instruments are
unpacked on a flat, sufficiently dimensioned
and non-slip surface.
Refer also to the separately provided
User Manual for the TL ST transmitted
light base.
Focusing drive and column
Never unscrew the 3 screws
on the right side of the
focusing column.
1. Unscrew the extension plate from the base
using the Allen key provided.
2. Attach your focusing drive column to the
bottom using the 4 Allen screws.
3. Reattach the adapter plate to its original
position using the 6 Allen screws.

Leica S Series User Manual 19
Optics carrier
1. Insert the optics carrier carefully in the
microscope carrier and fasten it in the
desired position with the clamping screw.
Additional objective (optional)
1. Screw the desired objective counterclock-
wise into the optics carrier.
Protective objective glass (optional)
1. Screw the objective protective glass directly
onto the StereoZoom® or on the additional
objective.
Optics Carrier and Additional Objective

Leica S Series User Manual 20
The optional graticules enable measure-
ment and, in addition, provide valuable
information when comparing and capturing
still images of the specimens. Insert the grati-
cule before you set the eyepiece in place.
Available graticules
The following graticules and stage micrometers
for calibrating may be ordered:
▶Graticule, 10 mm/0.1 mm
▶Graticule, 5 mm/0.1 mm
▶Graticule, 5 mm/0.05 mm
▶Graticule, 100 div./0.002"
▶Graticule, 100 div./0.001"
▶Graticule, 150 div./0.0005"
▶Crosshair
▶Stage micrometer, 50 mm, 0.1/0.01 mm
graduation
▶Stage micrometer, 1", 0.001" graduation
Available Graticules