LW Scientific Revelation III LED User manual

Introduction
The LW Scientific Revelation III Microscope is our best-selling medical-grade compound microscope, popular in physician and
veterinary offices as well as universities and medical schools. Its crisp optics, sturdy design and proven track record make the
Revelation III the best choice for superior performance at an economical price. The Revelation lll Microscope is intended for
use as a biological microscope in a professional environment in accordance with the guidelines set forth in this operations
manual.
Instruction Manual
Revelation III LED
Model pictured:
Revelation III LED
Not all features available on all models -
see back page for model specifications.
A
C
D
E
H
M
K
J
I
Q
PO
N
S
G
L
F
A Eyepieces
B Single Diopter Adjustment
CNose Piece
D Objectives
E Slide Holder & Slide Finger
F Substage Abbe Condenser
G Flip-out Filter Holder
H Base Condenser
I On/Off Switch
J Brightness Control
K X/Y Axis Stage Controls
L Substage Iris Diaphragm
M Stage
N Head Retention Screw
O Friction Adjustment Ring
P Coarse Focus
Q Fine Focus
R Stage Stop Lever
SMicroscope Head
(Binocular model shown)
R
B
Portable, Rechargeable Model Available
R3M-BN4A-DALP: Portable Revelation III, Binoc with 5V DC Base, USB Cord, & Battery
R3M-TN4A-DALP: Portable Revelation III, Trinoc with 5V DC Base, USB Cord, & Battery
Powered by USB Power Adapter Powered by Laptop USB Port Powered by Rechargeable Battery

Remove the body of the microscope and place it on a sturdy, dust-free surface. Remove the plastic caps in the nose piece.
Install the Objectives in such a way that when you turn the Nose Piece clockwise, you are moving from the 4x, to 10x to 40x
and finally to the 100x objective.
Remove the Microscope Head from the Styrofoam carton and pull off the protective covers from the Eyepieces and head
mount. Insert the head mount into the upper arm of the body. Using the Head Retention Screw, secure the Microscope
Head in place. Note: Do not over-tighten.
Insert the 10x eyepieces.
For protection during shipping your Revelation III is shipped with the stage locked in low position. To disengage the lock,
pull the Stage Lock Lever towards the rear and downward. The Stage should now move upward freely.
Attach the power cord.
Unpacking and Setup
Assembly
LW Scientific packs each Revelation III Microscope with utmost care. Examine
the outer and inner containers for any visual damage. Retain all of the packing
material until you have examined and tested your new microscope. If there is
damage, please contact the shipping company, as our warranty does not
cover shipping damage. If you are uncertain who the shipper is, contact the
distributor from whom you purchased the microscope. Please retain all packaging material for future use. Carefully unpack**
your Revelation III Microscope using the following checklist for all the parts and accessories:
1 - Microscope body with Abbe condenser
2 - 10x eyepieces
1 - Binocular or Trinocular head (Seidentopf style)
1 - Mirror and attachment device for field use
2 - Filters (blue & green)
4 - Objectives 4X, 10X, 40XR, 100XR (oil)
1 - 3-Prong power cord (For portable model: USB cord with powerblock, plus rechargeable battery)
1 - Immersion oil
1 - Dust cover
1 - Spare Fuse
**Note: Some parts may be packed in the outer recesses of the
Styrofoam blocks
Power
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5
3
1
4
If you suspect faulty electronics, call LW Scientific’s technical service department at 800-726-7345.
Input: Auto-switching 100V-240V AC / 50-60Hz (For portable model: 5 volt DC power from USB port or battery)
Bulb: 3w LED
Fuse: T1A
When purchased from LW Scientific or from an authorized distributor, LW Scientific Professional Stereoscopes and Laboratory
Microscopes have a life time warranty against defects in materials and workmanship and one (1) year warranty on electronic
components. This warranty is not valid on normal wear and tear, cosmetic damages caused by chemicals, solvents, and/or
cleaning solutions, as well as acts of God.
Please register your product online at: www.lwscientific.com/warranty_form.
Important: Warranty information must be completed within 30 days of purchase.
P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30046

Operation
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Once you have assembled all the parts and allowed your microscope to come to room temperature, then plug in the
power cord. Note: Excess cold can fog lenses and cause lamp to fail.
Turn the light on using the green On/Off Switch on the right side of the base. Next, adjust the light intensity using the Bright-
ness Control located on the right side just behind the On/Off Switch.
In order to become acquainted with the controls, choose a specimen slide with which you are familiar. For example, an
old hematology slide or a commercially prepared slide. Place the slide into the Slide Holder by pushing back on the thumb
guard to open the Slide Finger. The Slide Finger closes slowly to eliminate the possibility of chipping the corner of your slide
when it closes.
Move the slide to the center of the Stage, by turning the X/Y Axis Stage Control, located just below the Stage. The X/Y
Axis Stage Control allows you to move the slide on the X-Y axis (forward/backward and left/right).
The Substage Iris Diaphragm should be set to match the aperture of the objective for maximum resolution under each
objective power. You should begin with the 4x or 10x objective.
4x objective 1.0 N.A. (nearly closed) 40x objective - 0.65 N.A. (halfway closed)
10x objective - 0.25 N.A. (1/4 closed) 100x objective - 1.25 N.A. (wide open)
Once you are comfortably seated, look into the oculars and move the eyepiece tubes together or apart until you see only
one complete circle of light. You have now adjusted your interpupillary distance.
Using the 4x or 10x objectives and the Coarse and Fine Focus Adjustment knobs, bring the specimen into focus. Now,
move the 40x objective into place. You will feel a “clicking” action when the Objective is seated properly. Again, adjust
focus for best image. You should also adjust the Substage Iris Diaphragm (as listed above) for the best contrast and resolu-
tion.
Diopter Adjustment:If you are using a binocular microscope, it is necessary to adjust the normal difference in vision
between your two eyes. This is a simple, but critical adjustment! Close your left eye and look into the right ocular with your
right eye. Adjust the focus to give you the best image. Now look at the ocular tube on the left. You will see the left ocular
tube has a built-in Single Diopter Adjustment. Now close your right eye and look with your left eye into the left ocular. Using
the Single Diopter Adjustment on the left ocular tube, adjust the focus until you see a clear, focused field.
Friction Adjustment: With repeated use and wear, the Stage may drift out of focus. If this happens, tighten the Friction
Adjustment Ring (located on the right side of the microscope between the Coarse Focus and the body of the micro-
scope). If the Coarse Focus is hard to turn, you may choose to loosen the Friction Adjustment Ring.
Stage Stop Lever:To help prevent the Stage from making contact with the Objectives, the Revelation III Microscope is
equipped with an adjustable Stage Stop Lever. Rotate the 100x oil objective into place and put a slide into the Slide
Holder. Slowly raise the Stage, stopping when the slide makes contact with the Objective. Now, turn the Stage Stop Lever
in a clockwise direction toward you. The Stage Stop Lever is located on the left side of the microscope between the
Coarse Ajustment and the body of the microscope.
P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30046
8

Maintenance
Specifications
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1Always cover your microscope with the dust cover when not in use.
When cleaning the lenses, use lens paper or a cotton swab dipped in lens cleaning solution.
Excess oil should be cleaned off your 100x objective and stage at once. An alcohol pad is best for removing oil from the
stage and on the other metal parts, but is not recommended for use on the lenses. Use lens cleaning solution and lens
paper to clean off your objectives.
Dust in the nosepiece or ocular tubes should be blown out using filtered air. Canned air dusters work well for this job.
Whenever you remove an objective, we recommend that you place it back into the original plastic shipping vial until
ready to be placed back on the microscope. SCREW THE OBJECTIVE SECURELY INTO THE CAP OF THE HOLDER - DO NOT
DROP OBJECTIVE LOOSELY INTO CONTAINER.
To keep your microscope in top condition for years, LW Scientific recommends that you have the microscope
professionally serviced once a year. Warning: The 40x objective is not sealed for oil immersion. Damage to the 40x
objective due to oil immersion is not covered under warranty.
Nosepiece
Quadruple hole multiple ball bearing
Head
Binocular (Seidentopf)
Inclined 30°, rotates 360°
Single diopter adjustment
10X/18 wide-field eyepieces
Monocular or Trinocular available
Illumination
Moveable Abbe Condenser (NA 1.25)
Iris Diaphragm
Swing-out filter holder with blue and green filters
LED light
Objectives
DIN Achromatic or DIN Plan
4X N.A. 0.10
10X N.A. 0.25
40XR N.A. 0.65
100XR N.A. 1.25 (oil immersion)
20X, 50X oil Plan, and 60XR available
Adjustment Controls
Dimensions and Weight
Weight: 14 lbs / 6.4 kg
Height: 15" / 380 mm
Length: 9" / 230 mm
Width: 7" / 178 mm
Shipping weight: 17 lbs/ 8kg
Stage
Mechanical stage
(140 mm x 130 mm) – coaxial drive controls
Range of traverse is 78 mm x 50 mm
Acid and reagent resistant finish
Operating Environment
Indoor Use Only
Ambient temperature: 5˚ to 40˚C (41˚ to 104˚F)
MKT-7.5.3-L-060 | Rev 4
P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30046
Size N.A. Mag. Field of View
4X 0.10 40X 4.5 mm
10X 0.25 100X 1.8 mm
20X 0.40 200X 0.9 mm
40XR 0.65 400X 0.45 mm
50XR 0.95 500X 0.36 mm
60XR 0.85 600X 0.3 mm
100XR 1.25 1000X 0.18 mm
Objectives: The following numbers are
based on use with 10x/18 eyepieces.
Construction
Rugged alloy
Eyepiece: Interpupillary distance adjustment
Binocular: 48-75mm
Trinocular: 55-75 mm
Stage Controls: Knobs allow movement of slide on X-Y axis
Etched vernier scales
Coarse Adjustment: Range of 30 mm
Fine Adjustment: Graduation of 2µm
Variable Light Adjustment