LW Scientific Revelation III LED User manual

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P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30045P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30045
Instruction Manual
A Single Diopter Adjustment
B Binocular Head
C Head Retention Screw (side)
D Stage Stop Lever
E Friction Adjustment
F Coarse Focus
G Fine Focus
H X/Y Axis Stage Controls
I Brightness Control
J On Switch
K Base Condenser
L Flip-out Filter Holder
M Substage Iris Diaphragm
N Substage Abbe Condenser
O Stage
P Slide Holder
Q Objectives
R Nose Piece
S Eyepieces
Model pictured:
Maintenance
Ask your
authorized LWS
dealer about
additional
accessories
Upgrades:
Recommended
Revelation III
Revelation III LED
Carry Cases Camera Attachments
Phase and Dark Field
Warranty
Specifications
The Revelation III is covered by a limited lifetime warranty on materials and workmanship and
1-year warranty on electronics from date of purchase. If there is any indication of a problem,
contact LW Scientific. Operating the unit after noticing a problem could compound a simple
problem and cause an unneccessary expense to the owner. LW Scientific support staff will
troubleshoot problems over the phone and attempt to solve problems in the most expedient manner. This may include
sending parts that can easily be installed by the user, or directing the user through a simple adjustment to the unit.
Making repairs to the unit without authorization from LW Scientific will void the warranty.
If the unit must be shipped in for repair, LW Scientific will issue a Return Merchandise Authorization (RMA) number. You will
need to provide the serial number and have either a warranty card on file at LW Scientific or proof of purchase.
Introduction
The LW Scientific Revelation III microscope is our best-selling medical-grade compound microscope, popular in physician
and veterinary offices as well as universities and medical schools. Its crisp optics, sturdy design and proven track record
make the Revelation III the best choice for superior performance at an economical price. The Revelation 3 microscope is
intended for use as a biological microscope in a professional environment in accordance with the guidelines set forth in
this operations manual.
Not all features available on all models -
see back page for model specifications.
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1Always cover your microscope with the dust cover when not in use.
When cleaning the lenses, use lens paper or a cotton swab dipped in lens cleaning solution.
Excess oil should be cleaned off your 100x objective and stage at once. An alcohol pad is best for removing oil from
the stage and on the other metal parts, but is not recommended for use on the lenses. Use lens cleaning solution and
lens paper to clean off your objectives.
Dust in the nosepiece or ocular tubes should be blown out using filtered air. Canned air dusters work well for this job.
Whenever you remove an objective, we recommend that you place it back into the original plastic shipping vial until
ready to be placed back on the microscope. SCREW THE OBJECTIVE SECURELY INTO THE CAP OF THE HOLDER - DO NOT
DROP OBJECTIVE LOOSELY INTO CONTAINER.
To keep your microscope in top condition for years, LW Scientific recommends that you have the microscope
professionally serviced once a year. Warning: The 40x objective is not sealed for oil immersion. Damage to the 40x
objective due to oil immersion is not covered under warranty.
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Construction
Rugged alloy
Nosepiece
Quadruple hole multiple ball bearing
Head
Binocular (Seidentopf)
Inclined 30°, rotates 360°
Single diopter adjustment
10X/18 wide-field eyepieces
Monocular or Trinocular available
Illumination
Moveable Abbe Condenser (NA 1.25)
Iris Diaphragm
Swing-out filter holder with blue and green filters
LED light
Objectives
DIN Achromatic or DIN Plan
4X N.A. 0.10
10X N.A. 0.25
40XR N.A. 0.65
100XR N.A. 1.25 (oil immersion)
20X, 50X oil Plan, and 60XR available
Adjustment Controls
Eyepiece: Interpupillary distance adjustment 55-75 mm
Stage Controls: Knobs allow movement of slide on X-Y axis
Etched vernier scales
Coarse Adjustment: Range of 30 mm
Fine Adjustment: Graduation of 2µm
Variable Light Adjustment
Size N.A. Mag. Field of View
4X 0.1040X4.5 mm
10X 0.25 100X 1.8 mm
20X 0.40 200X 0.9 mm
40XR0.65400X 0.45 mm
50XR0.95500X 0.36 mm
60XR 0.85 600X 0.3 mm
100XR1.251000X 0.18 mm
Objectives: The following numbers are
based on use with 10x/18 eyepieces.
Dimensions and Weight
Weight: 14 lbs / 6.4 kg
Height: 15" / 380 mm
Length: 9" / 230 mm
Width: 7" / 178 mm
Shipping weight: 17 lbs/ 8kg
Stage
Mechanical stage
(140 mm x 140 mm) – coaxial drive controls
Range of traverse is 73 mm x 43 mm
Acid and reagent resistant finish
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R3M-B04A-DAL3
Operating Environment
Indoor Use Only
Ambient temperature: 5˚ to 40˚C (41˚ to 104˚F)

P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30045
Unpacking and Setup
Assembly
Operation
LW Scientific packs each Revelation III Microscope with utmost care.
Examine the outer and inner containers for any visual damage. Retain all of
the packing material until you have examined and tested your new
microscope. If there is damage, please contact the shipping company, as
our warranty does not cover shipping damage. If you are uncertain who the shipper is, contact the distributor from whom
you purchased the microscope. Please retain all packaging material for future use. Carefully unpack** your Revelation III
Microscope using the following checklist for all the parts and accessories:
1 - Microscope body with Abbe condenser
2 - 10x eyepieces
1 - Binocular head (Seidentopf style)
1 - Mirror and attachment device for field use
2 - Filters (blue & green)
4 - Objectives 4X, 10XR, 40XR, 100XR (oil)
1 - Power Adapter
1 - Warranty Card
1 - Immersion oil
1 - Dust cover
**Note: Some parts may be packed in the
outer recesses of the Styrofoam blocks
Power
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1Remove the body of the microscope and place it on a sturdy, dust-free surface. Remove the plastic plugs in the nose
piece. Install the objectives in such a way that when you turn the nosepiece clockwise, you are moving from the 4x,
Insert the 10x eyepieces.
Attach the power adapter.
4For protection during shipping your Rev III is shipped with the stage locked in low position. To disengage the lock,
pull the STAGE LOCK LEVER towards the rear and downward. The stage should now move upward freely.
Remove the microscope head from the Styrofoam carton and pull off the protective covers from the
eyepiece tubes and head mount. Insert the head mount into the upper arm of the body. Using the
head retention screw, secure the head in place. Note: Do not over-tighten.
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Once you have assembled all the parts and allowed your microscope to come to room temperature, plug the
power adapter into the microscope and then into the appopriate AC outlet. Note: Excess cold can fog lenses
and cause lamp to fail.
Turn the light on using the black on/off switch on the right side of the base. Next adhust the light intensity using
the brightness control wheel located on the right side just behind the on/off switch.
In order to become acquainted with the controls, choose a specimen slide with which you are familiar. For
example, an old hematology slide or a commercially prepared slide. Place the slide into the slide holder by
pushing back on the thumb guard to open the slide finger. The slide finger closes slowly to eliminate the
possibility of chipping the corner of your slide when it closes.
Move the slide to the center of the stage, by turning the stage control knobs, located just below the stage.
These knobs allow yo to move the slide on the X-Y axis (forward/backward and left/right).
The sub-stage iris should then be set to match the aperture of the objective for maximum resolution under each
objective power. You should begin with the 4x or 10x objective.
4x objective 1.0 N.A. (nearly closed) 40x objective - 0.65 N.A. (halfway closed)
10x objective - 0.25 N.A. (1/4 closed) 100x objective - 1.25 N.A. (wide open)
Insert the filter of your choice into the swing-out filter holder beneath the Abbe condenser. Note that many
customers prefer to use the blue filter for routine use.
Once you are comfortably seated, look into the oculars and move the eyepiece tubes together or apart until
you see only one complete circle of light. You have now adjusted your interpupillary distance.
Using the 4x or 10x objectives and the coarse and fine adjustment knobs, bring the specimen into focus. Now,
move the 40x objective into place. You will feel a “clicking” action when the objective is seated properly.
Again, adjust focus for best image. You should also adjust the iris diaphragm (as listed above) for the best
contrast and resolution.
Diopter Adjustment: Since you are using a binocular microscope, you have to adjust the normal difference in
vision between your two eyes. This is a simple but critical adjustment! Close your left eye and look into the right
ocular with your right eye. Adjust the focus to give you the best image. Now look at the ocular tube on the left.
You will see that the left ocular tube has a built-in adjustment ring. Now close your right eye and look with your
left eye into the left ocular. Using the diopter adjustment ring on the left ocular tube, adjust the focus until you
see a clear, focused field.
Friction Adjustment: With repeated use and wear, the stage may drift out of focus. If this happens, you need
only to tighten the tension control ring (located on the right side of the microscope between the coarse
adjustment and the body of the microscope). If the coarse focus is hard to turn, you may choose to loosen the
friction adjustment.
Stage Stop Lever: To help prevent the stage from hitting the objectives, the Revelation III Microscope is
equipped with an adjustable stage stop. Rotate the 100x oil objective into place, and put a slide into the slide
holder. Slowly raise the stage, stopping when the slide makes contact with the objective. Now, turn the stage
stop lever in a clockwise direction toward you. The stage stop lever is located on the left side of the micro-
scope between the coarse adjustment and the body of the microscope.
If you suspect faulty electronics, call LW Scientific’s technical service department at 800-726-7345.
USE ONLY SUPPLIED POWER ADAPTER
Power Adapter:
Input: 90-240V / 50-60 Hz Auto-Switching
Output: 12V 1A
Microscope:
Input: 12V 1A