Merck Spectroquant prove 100 User manual

0QFSBUJOH.BOVBM

4QFDUSPRVBOUn1SPWF

4QFDUSPRVBOUn1SPWF4QFDUSPQIPUPNFUFS]]

7FSTJPO֮

7FSTJPO֮ 

$POUFOUT

 4QFDUSPQIPUPNFUFST

 1IPUPNFUSZ

 5IF4QFDUSPQIPUPNFUFST

 1IPUPNFUSJD5FTU,JUT 

 #BTJD1SJODJQMF

 4QFDUSPRVBOUn$FMM5FTUT

 4QFDUSPRVBOUn3FBHFOU5FTUT

 /PUFTGPS1SBDUJDBM6TF

 .FBTVSJOH3BOHF 

 *OꯏVFODFPGQ)

 *OꯏVFODFPG5FNQFSBUVSF

 5JNF4UBCJMJUZ 

 *OꯏVFODFPG'PSFJHO4VCTUBODFT

 %PTJOHUIF3FBHFOUT

 4IFMGMJGFPGUIF3FBHFOUT

 4BNQMF1SFQBSBUJPO 

 5BLJOH4BNQMFT

 1SFMJNJOBSZ5FTUT

 %JMVUJPO

 'JMUSBUJPO 

 )PNPHFOJ[BUJPO 

 %FDPNQPTJUJPO

 1JQFUUJOH4ZTUFN 

 "OBMZUJDBM2VBMJUZ"TTVSBODF"2" 

 2VBMJUZ$POUSPMBUUIF.BOVGBDUVSFS

 2VBMJUZ$POUSPMGPSUIF6TFS 

 $IFDLJOHUIF4QFDUSPQIPUPNFUFS

 $IFDLJOHUIF0WFSBMM4ZTUFN

 $IFDLJOHUIF1JQFUUFT

 $IFDLJOH5IFSNPSFBDUPST

 5FTUJOHGPS)BOEMJOH&SSPST

 %FUFSNJOBUJPOPG4BNQMF*OꯏVFODFT.BUSJY&GGFDUT

 %FꯎOJUJPOPG&SSPST

 0WFSWJFX 

 4DPQFPG%FMJWFSZ

 0WFSWJFXPGUIF*OTUSVNFOU

 %JTQMBZBOE6TFS*OUFSGBDF

 4BGFUZ 

 *OUFOEFE6TF

 (FOFSBM4BGFUZ*OTUSVDUJPOT

 'VODUJPOBOE0QFSBUJPOBM4BGFUZ

 5BSHFU(SPVQBOE6TFS2VBMJꯎDBUJPO

 )BOEMJOH)B[BSEPVT4VCTUBODFT

7FSTJPO֮

 (FUUJOH4UBSUFE 

 (FOFSBM/PUFTPO)BOEMJOH

 *OJUJBM4FUVQ

 $POOFDUJOHUIF1PXFS4VQQMZ

 'JSTU1PXFSPO 

 -BOHVBHF4FUVQ 

 %BUF5JNFBOE$PVOUSZTQFDJꯎD4FUUJOHT

 4FMGUFTU

 $POOFDUJOH0QUJPOBM1FSJQIFSBM%FWJDFT 

 $PNNVOJDBUJPO1PSUT 

 1SJOUFS 

 64#.FNPSZ%FWJDF

 #BSDPEF3FBEFS

 0QFSBUJPO 

 4XJUDIJOHUIF4QFDUSPQIPUPNFUFS0OPS0GG

 4ZTUFN4FUVQ

 *OGPSNBUJPO 

 *OUFSGBDF4FUVQ

 3FHJPO4FUVQ

 2VBMJUZ4FUVQ

 "VUPNBUJPO4FUVQ

 6TFS.BOBHFNFOU

 4FSWJDF

 6QEBUFT

 .FBTVSFNFOUT

 1FSGPSNJOHB.FBTVSFNFOU

 ;FSP"EKVTUNFOU

 /PUFTPO;FSP"EKVTUNFOU

 8IFOUP3FQFBUUIF;FSP"EKVTUNFOU 

 ;FSP"EKVTUNFOUGPS6TFSEFꯎOFE$PODFOUSBUJPO.FUIPET

 ;FSP"EKVTUNFOU1SPDFEVSFGPS1SFQSPHSBNNFE$PODFOUSBUJPO.FUIPET

 ;FSP"EKVTUNFOUGPS"EIPD"CTPSCBODF5SBOTNJTTJPO.FBTVSFNFOUT

 ;FSP"EKVTUNFOU1SPDFEVSFGPS"EIPD"CTPSCBODF5SBOTNJTTJPO.FBTVSFNFOUT

 ;FSP"EKVTUNFOUGPS"EIPD,JOFUJD.FBTVSFNFOUT

 ;FSP"EKVTUNFOU1SPDFEVSFGPS"EIPD,JOFUJD.FBTVSFNFOUT

 ;FSP"EKVTUNFOUGPS"EIPD4QFDUSB

 ;FSP"EKVTUNFOU#BTFMJOF1SPDFEVSFGPS"EIPD4QFDUSVN.FBTVSFNFOU

 ;FSP"EKVTUNFOUGPS6TFSEFꯎOFE,JOFUJD.FUIPET

 ;FSP"EKVTUNFOU1SPDFEVSFGPS6TFSEFꯎOFE,JOFUJD.FUIPET

 ;FSP"EKVTUNFOUGPS6TFSEFꯎOFE4QFDUSVN.FUIPET

 ;FSP"EKVTUNFOU#BTFMJOF1SPDFEVSFGPS6TFSEFꯎOFE4QFDUSVN.FUIPET

7FSTJPO֮ 

 .FUIPE-JTU

 4FMFDUJOHB.FUIPE.BOVBMMZ

 4FBSDIJOHBOE'JMUFSJOHUIF.FUIPE-JTU

 1SPHSBNNJOHB6TFSEFꯎOFE.FUIPE 

 6TFSEFꯎOFE$PODFOUSBUJPO.FUIPET

 $BMJCSBUJPO%BUBBOE$BMJCSBUJPO'VODUJPOGPS4JOHMFXBWFMFOHUI.FUIPET

 1SPHSBNNJOH.PEJGZJOH6TFSEFꯎOFE.FUIPET4JOHMF8BWFMFOHUI

 $BMJCSBUJPO%BUBBOE$BMJCSBUJPO'VODUJPOGPS.VMUJXBWFMFOHUI.FUIPET

 1SPHSBNNJOH.PEJGZJOH6TFSEFꯎOFE.FUIPET.VMUJXBWFMFOHUI

 1SPHSBNNJOHB6TFSEFꯎOFE4QFDUSVN.FUIPE

 1SPHSBNNJOHB6TFSEFꯎOFE,JOFUJD.FUIPE

 $PQZJOH%VQMJDBUJOHB6TFSEFꯎOFE.FUIPE

 .PEJGZJOHB6TFSEFꯎOFE.FUIPEGSPNUIF.FUIPE-JTU

 %FMFUJOHB6TFSEFꯎOFE.FUIPEGSPNUIF.FUIPE-JTU

 &YQPSU6TFSEFꯎOFE.FUIPETUPB64#.FNPSZ%FWJDF

 .FBTVSJOHJO$PODFOUSBUJPO.PEF

 .FBTVSJOH$FMM5FTUTXJUIB#BSDPEF

 .FBTVSJOH3FBHFOU5FTUTXJUI"VUP4FMFDUPS

 .FBTVSJOH3FBHFOUGSFF5FTUTBOE6TFSEFꯎOFE.FUIPET

 &YDFFEJOHUIF6QQFSPS-PXFS-JNJUTPGUIF.FBTVSJOH3BOHF

 .FUIPETQFDJꯎD4FUUJOHTGPS$PODFOUSBUJPO.PEF

 .FBTVSJOH%JMVUFE4BNQMFT

 4BNQMF#MBOL7BMVF

 3FBHFOU#MBOL7BMVF

 "VUPNBUJD5VSCJEJUZ$PSSFDUJPO

 6TFS3FDBMJCSBUJPO4UBOEBSE"EKVTUNFOU

 .BUSJY$IFDL

 "EIPD.FBTVSFNFOU 

 "EIPD"#453"/4.FBTVSFNFOU

 "EIPD4QFDUSVN.FBTVSFNFOU 

 "EIPD,JOFUJD.FBTVSFNFOU 

 4QFDUSVN 

 (FOFSBM*OGPSNBUJPO 

 3FDPSEJOHUIF4QFDUSVN 

 &WBMVBUJOHB4QFDUSVN 

 ,JOFUJDT 

 (FOFSBM*OGPSNBUJPO 

 3FDPSEJOH,JOFUJDT 

 &WBMVBUJOHB,JOFUJD 

7FSTJPO֮

 "2""OBMZUJDBM2VBMJUZ"TTVSBODF 

 4QFDUSPQIPUPNFUFS.POJUPSJOH"2" 

 5PUBM4ZTUFN.POJUPSJOH"2" 

 "2"0WFSWJFX 

 1FSGPSN"2"4UBUVT$IFDL 

 1FSGPSN"2"4UBUVT$IFDL 

 "2"4FMFDUJPO-JTU 

 "DUJWBUJOHBOE%FBDUJWBUJOHBO"2"5FTU 

 &EJUJOHBO"2"5FTU 

 1FSGPSNJOHBO"2"5FTU 

 $SFBUJOHB6TFSEFꯎOFE"2"5FTU 

 "2"4FMFDUJPO-JTU 

 "DUJWBUJOHBOE%FBDUJWBUJOHBO"2"5FTU 

 &EJUJOHBO"2"5FTU 

 1FSGPSNJOHBO"2"5FTU 

 1FSGPSN1JQF$IFDL 

 5JNFS 

 3FTVMUTBOE.FBTVSFNFOU%BUBTFUT

 %JTQMBZJOHUIF3FTVMUT 

 4IPX%FUBJMTPG0OF3FTVMU 

 'JMUFSJOH3FTVMUTUP'VSUIFS1SPDFTT.FBTVSFNFOU%BUBTFUT 

 1BOPSBNB֮7BMVF$POUSPM$BSE 

 1SJOU3FTVMUTBOE.FBTVSFNFOU%BUBTFUT 

 &YQPSU3FTVMUTBOE.FBTVSFNFOU%BUBTFUT 

 6TFS.BOBHFNFOU 

 "DUJWBUJOH%FBDUJWBUJOHUIF6TFS.BOBHFNFOU'VODUJPO 

 $SFBUFBO"ENJOJTUSBUPS6TFS"DDPVOU 

 &EJUPS%FMFUFB6TFS 

 -PHJO-PHPVU 

 $IBOHF1BTTXPSEXJUI6TFS3JHIUT0OMZ 

.BJOUFOBODFBOE$MFBOJOH 

 &YDIBOHJOHUIF#VGGFS#BUUFSZ 

 &YDIBOHJOHUIF)BMPHFO-BNQ1SPWF 

 $MFBOJOH 

 $MFBOJOHUIF)PVTJOHBOE%JTQMBZ 

 $MFBOJOHUIF$FMM$PNQBSUNFOU 

 $MFBOJOHUIF$FMM$PNQBSUNFOU$PWFSBOEUIF3FBS$BWJUZ 

 $MFBOJOHUIF%FUFDUPS-FOT 

7FSTJPO֮ 

&SSPS$BVTFTBOE5SPVCMFTIPPUJOH 

5FDIOJDBM%BUB 

 4QFDUSPRVBOUn1SPWF 

 4QFDUSPRVBOUn1SPWF 

 4QFDUSPRVBOUn1SPWF 

"DDFTTPSJFTBOE5FTU.FEJB 

 "DDFTTPSJFT 

 0QUJPOBM&RVJQNFOU$POOFDUJPO$BCMFT 

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"QQFOEJY 

-JTUPG4NBSU*DPOTPO%JTQMBZ 

 -JTUPG5SBEFNBSLT 

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BOBMZTJTTZTUFNEJGGFSGSPNDPOWFOUJPOBMTQFDUSPQIPUPNFUFSTJO

UIFGPMMPXJOHJNQPSUBOUBTQFDUT

ֽ 5IFDBMJCSBUJPOGVODUJPOTPGBMMUFTULJUTBSFFMFDUSPOJDBMMZTUPSFE

ֽ 5IFNFBTVSFNFOUWBMVFDBOCFJNNFEJBUFMZSFBEPGGGSPNUIF

EJTQMBZJOUIFEFTJSFEGPSN

ֽ 5IFNFUIPEGPSUIFUFTULJUT$FMM5FTUTBOESFBHFOUUFTUT

CFMPOHJOHUPUIF4QFDUSPRVBOUnBOBMZTJTTZTUFNJTBVUPNBUJ

DBMMZTFMFDUFEWJBUIFTDBOOJOHPGUIFCBSDPEF

ֽ "MMDFMMTGPSNBUTVTFEBSFBVUPNBUJDBMMZJEFOUJꯎFEBOEUIF

DPSSFDUNFBTVSJOHSBOHFJTTFMFDUFEBVUPNBUJDBMMZ

ֽ *OTUSVNFOUTVQQPSUFE"2"FOTVSFTUIBUNFBTVSFNFOUSFTVMUT

DBOCFVTFEBTTFDVSFSFQSPEVDJCMFBOESFDPHOJ[FEBOBMZUJDBM

SFTVMUT

ֽ /FXNFUIPETDBOCFEPXOMPBEFEGSPNUIFJOUFSOFUTJUF

XXXTFSWJDFUFTULJUTDPNBOEQFSNBOFOUMZTUPSFEJOUIF

JOTUSVNFOU

'PSUFDIOJDBMEBUBBOEJOTUSVDUJPOTGPSVTFQMFBTFSFGFSUP

4FDUJPOBOEUIF"OBMZUJDBM1SPDFEVSFTBOE"QQFOEJDFT

5IFZDBOBMTPCFGPVOEJOUIFJOUFSOFU

4QFDUSPQIPUPNFUFST֗5IF4QFDUSPQIPUPNFUFST

7FSTJPO֮

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#ZNFBOTPGSFBHFOUTUIFDPNQPOFOUPGBTBNQMFUPCFBOBMZ[FE

JTDPOWFSUFEJOUPBDPMPSFEDPNQPVOEJOBTQFDJꯎDSFBDUJPO5IF

SFBHFOUTPSSFBHFOUNJYUVSFTDPOUBJO֮JOBEEJUJPOUPUIFSFBHFOU

TFMFDUJWFGPSBQBSBNFUFSUPCFEFUFSNJOFE֮BOVNCFSPGBVYJM

JBSZTVCTUBODFTUIBUBSFFTTFOUJBMGPSUIFDPVSTFPGUIFSFBDUJPO

5IFTFJODMVEFGPSFYBNQMFCVGGFSTGPSBEKVTUJOHUIFQ)UPUIF

PQUJNBMWBMVFGPSUIFSFBDUJPOBOENBTLJOHBHFOUTUIBUTVQQSFTT

PSNJOJNJ[FUIFJOꯏVFODFPGJOUFSGFSJOHJPOT

5IFDPMPSSFBDUJPOTBSFJONPTUDBTFTCBTFEPOTUBOEBSEJ[FE

BOBMZUJDBMNFUIPETTQFDJꯎDBMMZPQUJNJ[FEJOUFSNTPGFBTFPGVTF

BMPXXPSLJOHFGGPSUBOETIPSUFSSFBDUJPOUJNFT'VSUIFSNPSF

NFUIPETDJUFEJOUIFMJUFSBUVSFPSEFWFMPQFECZPVSTFMWFTBSFBMTP

VTFE%FUBJMTPOUIFSFTQFDUJWFSFGFSFODFQSPDFEVSFTBSFTUBUFEJO

UIFQBDLBHFJOTFSUPSFMTFJOUIFQBSBNFUFSPWFSWJFX

 4QFDUSPRVBOUn$FMM5FTUT

*EFOUJꯎDBUJPONBSLGPSUIFDPSSFDUJOTFSUJPO

JOUPUIFDFMMDPNQBSUNFOUPGUIFTQFDUSPQIPUPNFUFS

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%FTJHOBUJPOPGUFTULJU

3JTLQISBTFT

4QFDJBMDFMMJOPQUJDBMRVBMJUZ

-FBLQSPPGDBQ

#BSDPEFGPSJEFOUJꯎDBUJPOJOUIF/07"BOE1IBSP

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%FUBJMTSFHBSEJOHDPOUFOUT

)JHIMZQSFDJTFEPTBHFPGUIFSFBHFOU

 %CBSDPEFGPSJEFOUJꯎDBUJPOJOUIF1SPWFTQFDUSPQIPUP

NFUFST

"EEJUJPOBMSFBHFOUT

$FSUBJODFMMUFTUTFH$0%PSOJUSJUFBMSFBEZDPOUBJOBMMOFDFT

TBSZSFBHFOUTJOUIFDFMMTBOEUIFTBNQMFNVTUNFSFMZCFBEEFE

XJUIBQJQFUUF*OPUIFSUFTUTIPXFWFSGPSSFBTPOTPGDIFNJDBM

DPNQBUJCJMJUZJUJTOFDFTTBSZUPTFQBSBUFUIFUFTUJOUPUXPPSUISFF

EJGGFSFOUSFBHFOUNJYUVSFT*OTVDIDBTFTCFTJEFTUIFTBNQMFB

NFUFSFESFBHFOUNVTUBMTPCFBEEFE

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 4QFDUSPRVBOUn3FBHFOU5FTUT

5IFQSJODJQMFCFIJOEUIFSFBHFOUUFTUTJTUIBUUIFSFBHFOUTOFD

FTTBSZGPSUIFDPMPSSFBDUJPOBSFDPNCJOFEJOUIFGPSNPGMJRVJE

DPODFOUSBUFTPSTPMJETVCTUBODFNJYUVSFT"GFXESPQTPGUIF

SFBHFOUDPODFOUSBUFBSFBEEFEUPUIFTBNQMF5IJTNFBOTUIBU

UIFSFJTOPOFFEUPEJMVUFUIFTBNQMFXIJDIJOUVSOFOIBODFTUIF

TFOTJUJWJUZPGUIFEFUFDUJPO5IFQSPDFEVSFHFOFSBMMZVTFEJODMBT

TJDBMQIPUPNFUSZCZXIJDIUIFTBNQMFJTNBEFVQUPBEFꯎOFE

WPMVNFJOBWPMVNFUSJDꯏBTLJTEJTQFOTFEXJUI

5IFNFUIPEJTTFMFDUFEBVUPNBUJDBMMZCZNFBOTPGUIFTDBOOJOH

PGUIFCBSDPEFCZUIF"VUP4FMFDUPS"MMDFMMTGPSNBUTVTFEBSF

BVUPNBUJDBMMZJEFOUJꯎFEBOEUIFDPSSFDUNFBTVSJOHSBOHFJT

TFMFDUFEBVUPNBUJDBMMZ4VCTFRVFOUMZUIFSFTVMUJTBVUPNBUJDBMMZ

TIPXOPOUIFEJTQMBZ

1IPUPNFUSJD5FTU,JUT֗#BTJD1SJODJQMF

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1IPUPNFUSJD5FTU,JUT֗/PUFTGPS1SBDUJBM6TF

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 .FBTVSJOH3BOHF

Concentration

Absorbance

5IFJOUFOTJUZPGUIFDPMPSPGBTPMVUJPONFBTVSFEBTUIFBCTPSC

BODFJTQSPQPSUJPOBMUPUIFDPODFOUSBUJPOPGUIFSFTQFDUJWFBOB

MZUFPOMZXJUIJOBTQFDJꯎDSBOHF5IJTNFBTVSJOHSBOHFFGGFDUJWF

SBOHFJTFMFDUSPOJDBMMZTUPSFEJOUIFTQFDUSPQIPUPNFUFSTGPSFBDI

JOEJWJEVBMUFTULJU

#FMPXUIFTQFDJꯎFENFBTVSJOHSBOHFFJUIFSBEJGGFSFOUDFMMPS

FMTFBOPUIFSQSPDFEVSFNVTUCFVTFE5IFMPXFSMJNJUPGUIF

NFBTVSJOHSBOHFFJUIFSUBLFTUIFGPSNPGOPOMJOFBSJUZPGUIF

DBMJCSBUJPODVSWFBTTIPXOJOUIFꯎHVSFPSFMTFJTHJWFOCZ

UIFNFUIPEEFUFDUJPOMJNJU5IFNFUIPEEFUFDUJPOMJNJUPGBO

BOBMZUJDBMNFUIPEJTUIFMPXFTUDPODFOUSBUJPOPGUIFBOBMZUF

JORVFTUJPOUIBUDBOCFNFBTVSFERVBOUJUBUJWFMZXJUIBEFꯎOFE

EFHSFFPGQSPCBCJMJUZFH5IFVQQFSMJNJUPGUIFNFBT

VSJOHSBOHFJTUIFQPJOUBUXIJDIUIFMJOFBSDPSSFMBUJPOCFUXFFO

UIFDPODFOUSBUJPOBOEUIFBCTPSCBODFFOET*OTVDIBDBTFUIF

TBNQMFNVTUCFEJMVUFEBDDPSEJOHMZTPUIBUJUMJFTJEFBMMZJOUIF

NJEEMFPGUIFFGGFDUJWFSBOHFMFBTUFSSPSNFBTVSFNFOU

*OQIPUPNFUSZJUJTDPOWFOUJPOBMQSBDUJDFUPNFBTVSFBHBJOTU

UIFSFBHFOUCMBOLWBMVF)FSFUIFBOBMZTJTJTDBSSJFEPVUַCMJOEָ

JFXJUIPVUBOZBOBMZUFBEEFE*OTUFBEPGUIFTBNQMFWPMVNF

UIFDPSSFTQPOEJOHRVBOUJUZPGEJTUJMMFEPS%*XBUFSJTVTFE

5IJTSFBHFOUCMBOLWBMVFJTQSFTUPSFEJOUIFTQFDUSPQIPUP

NFUFSTCFMPOHJOHUPUIF4QFDUSPRVBOUnBOBMZTJTTZTUFNXIJDI

NFBOTUIBU֮EVFUPUIFIJHICBUDISFQSPEVDJCJMJUZ֮JUJTQPTTJCMF

UPEJTQFOTFXJUIBTFQBSBUFNFBTVSFNFOUPGUIFSFBHFOUCMBOL

"UUIFMPXFSMJNJUPGUIFNFBTVSJOHSBOHFUIFBDDVSBDZPGUIF

EFUFSNJOBUJPODBOCFFOIBODFECZQFSGPSNJOHUIFNFBTVSFNFOU

BHBJOTUBTFQBSBUFMZQSFQBSFESFBHFOUCMBOL*OTPNFDBTFTUIF

JOUFOTJUZPGUIFDPMPSPGUIFTPMVUJPOBOEUIVTUIFBCTPSCBODF

DBOESPQBHBJOXIFOWFSZIJHIDPODFOUSBUJPOTPGUIFBOBMZUFBSF

QSFTFOUTFFQBDLBHFJOTFSUPGUFTULJU

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Q)SBOHF5IFSFBHFOUTDPOUBJOFEJOUIFUFTULJUTQSPEVDFBO

BEFRVBUFCVGGFSJOHPGUIFTBNQMFTPMVUJPOTBOEFOTVSFUIBUUIF

Q)PQUJNBMGPSUIFSFBDUJPOJORVFTUJPOJTPCUBJOFE

4USPOHMZBDJEJDQ)BOETUSPOHMZBMLBMJOFQ)TBNQMF

TPMVUJPOTDBOQSFWFOUUIFQ)GSPNCFJOHBEKVTUFEUPBOPQUJNBM

SBOHFTJODFVOEFSDFSUBJODJSDVNTUBODFTUIFCVGGFSJOHDBQBDJUZ

PGUIFUFTULJUSFBHFOUTNBZOPUCFTVGꯎDJFOU"OZOFDFTTBSZDPS

SFDUJPOJTNBEFCZUIFESPQXJTFBEEJUJPOPGEJMVUFEBDJESFEVDFT

UIFQ)PSEJMVUFEMZFSBJTFTUIFQ)UFTUJOHUIFQ)XJUITVJUBCMF

JOEJDBUPSTUSJQTBGUFSFBDIESPQJTBEEFE5IFBEEJUJPOPGUIFBDJE

PSMZFSFTVMUTJOBEJMVUJPOPGUIFUFTUTPMVUJPO8IFOVQUPꯎWF

ESPQTBSFBEEFEUPNMPGTBNQMFUIFDIBOHFJOUIFWPMVNFDBO

CFOFHMFDUFETJODFUIFSFTVMUBOUFSSPSJTMPXFSUIBO

5IFBEEJUJPOPGMBSHFSRVBOUJUJFTTIPVMECFEVMZDPOTJEFSFECZ

BEKVTUJOHUIFTBNQMFWPMVNFBDDPSEJOHMZ

5IFTQFDJꯎFEQ)WBMVFTGPSUIFTBNQMFTPMVUJPOBOEXIFSFWFS

BQQMJDBCMFGPSUIFNFBTVSFNFOUTPMVUJPOBSFEFꯎOFEJOUIF

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Temperature (°C)

Absorbance

20 40

10 30

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5IFUFNQFSBUVSFPGUIFTBNQMFTPMVUJPOBOEUIFSFBHFOUTNBZ

IBWFBOFGGFDUPOUIFDPMPSSFBDUJPOBOEUIVTPOUIFNFBTVSF

NFOUSFTVMU5IFUZQJDBMUFNQFSBUVSFDPVSTFJTJMMVTUSBUFEJOUIF

ꯎHVSF

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NVTUCFSFDLPOFEXJUI5FNQFSBUVSFTFYDFFEJOHp$HFOFSBMMZ

JOꯏVFODFUIFTUBCJMJUZPGUIFDPNQPVOEUIBUJTGPSNFEJOUIF

SFBDUJPO5IFPQUJNBMUFNQFSBUVSFGPSUIFDPMPSSFBDUJPOJTTUBUFE

JOUIFQBDLBHFJOTFSUTPGUIFSFTQFDUJWF4QFDUSPRVBOUnUFTULJUT

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Reaction time (minutes)

Absorbance

30 60

.PTUPGUIFDPMPSSFBDUJPOTSFRVJSFBDFSUBJOUJNFUPSFBDIUIF

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SJHIUHJWFTBTDIFNBUJDJNQSFTTJPOPGBUZQJDBMUJNFDPVSTF5IF

CFIBWJPSPGSFMBUJWFMZJOTUBCMFDPMPSSFBDUJPOTXJUIUJNFJTTIPXO

CZUIFEPUUFEDVSWF5IFSFBDUJPOUJNFTQFDJꯎFEJOUIFXPSLJOH

JOTUSVDUJPOTSFGFSTUPUIFQFSJPEPGUJNFGSPNUIFBEEJUJPOPG

UIFMBTUSFBHFOUVOUJMUIFBDUVBMNFBTVSFNFOU*OBEEJUJPOUIF

QBDLBHFJOTFSUTGPSUIFJOEJWJEVBMUFTULJUTBMTPTUBUFUIFUJNF

JOUFSWBMJOXIJDIUIFNFBTVSFNFOUWBMVFEPFTOPUDIBOHF5IF

NBYJNVNUJNFJOUFSWBMJTNJOVUFTUIJTUJNFTIPVMEOPUCF

FYDFFEFEFWFOJOUIFDBTFPGTUBCMFDPMPSSFBDUJPOT

 *OꗝVFODFPG'PSFJHO4VCTUBODFT

'PSFJHOTVCTUBODFTJOUIFTBNQMFTPMVUJPODBO

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UIFSFBDUJPO

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SFBDUJPO

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SFTQFDUJWFQBDLBHFJOTFSUTGPSUIFNPTUJNQPSUBOUGPSFJHOJPOT

5IFUPMFSBODFMJNJUTIBWFCFFOEFUFSNJOFEGPSUIFJOEJWJEVBM

JPOTUIFZNBZOPUCFFWBMVBUFEDVNVMBUJWFMZ

4VJUBCJMJUZGPSVTFJOTFBXBUFS

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TVJUBCJMJUZPGUIFUFTUTJODPOOFDUJPOXJUITFBXBUFSBOEBMTPPO

UIFUPMFSBODFTGPSTBMUDPODFOUSBUJPOT

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SFBHFOUTPSSFBHFOUNJYUVSFTUIBUBSFGSFFꯏPXJOH*OBMMPUIFS

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SFBHFOUCPUUMF

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CPUUMFCFIFMEWFSUJDBMMZBOEUIBUUIFESPQTCFBEEFETMPXMZBQ

QSPYESPQQFSTFDPOE*GUIJTJTOPUPCTFSWFEUIFDPSSFDUESPQ

TJ[FBOEUIVTUIFDPSSFDUBNPVOUPGSFBHFOUBSFOPUBDIJFWFE

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RVBOUJUJFT*OUIFTFDBTFTUIFSFBHFOUCPUUMFTBSFOPUꯎUUFE

XJUIBESPQQFSJOTFSU

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CZUIFEPTFNFUFSJOHDBQ)PMEUIFSFBHFOUCPUUMFWFSUJDBMMZBOE

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PGUIFNFBTVSFNFOUTFSJFTTJODFUIFGVODUJPOPGUIFSFBHFOUJT

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5IF4QFDUSPRVBOUnUFTULJUTBSFJONPTUDBTFTTUBCMFGPSUISFFZFBST

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MJGFPGPSNPOUITPSNVTUFMTFCFTUPSFEJOBSFGSJHFSBUPS

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PGUIFQBDLBHFVOJUJTQSJOUFEPOUIFPVUFSMBCFM5IFTIFMGMJGFNBZ

CFDPNFSFEVDFEXIFOUIFSFBHFOUCPUUMFTBSFOPUSFDMPTFEUJHIUMZ

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UIPTFTQFDJꯎFE

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TBNQMFNBUSJY

5IFSFBEZUPVTFTBNQMFEFDPNQPTJUJPOQSPEVDUT4QFDUSP

RVBOUn$SBDL4FUBOEBSFTVJUFEGPSUIFQSFQBSBUJPOPG

UIFTBNQMFNBUFSJBMTGPSUIFEFUFSNJOBUJPOTTUBUFEJOUIFUBCMF

Decomposition

Measurement

Result A

A and B

identical?

No decomposition

necessary

Yes

Procedure

Measurement

Result B

Procedure

Decomposition

necessary

5IFEFDPNQPTJUJPOSFBHFOUTBSFBMSFBEZDPOUBJOFEJOUIFQBDLTPGUIF

SFTQFDUJWFDFMMUFTUT

%FDPNQPTJUJPODFMMTBSFJODMVEFEJOUIFQBDLFNQUZDFMMTBSFSFRVJSFEGPSUIF

EFDPNQPTJUJPOGPS$SBDL4FUTBOE

5IFEFDPNQPTJUJPOQSPDFTTFTBSFDBSSJFEPVUJOUIF4QFDUSP

RVBOUnUIFSNPSFBDUPSDBQBDJUZPSEFDPNQPTJUJPODFMMT

BUp$PSSFTQFDUJWFMZp$%FUBJMTSFHBSEJOHUIFIFBUJOH

UJNFTBOEGVSUIFSUSFBUNFOUDBOCFGPVOEJOUIFQBDLBHFJOTFSUT

DPOUBJOFEJOUIF4QFDUSPRVBOUn$SBDL4FUQBDLT

*OUIFFWFOUUIBUUIFTBNQMFUPCFBOBMZ[FEJTBIJHIMZDPOUBN

JOBUFENBUFSJBMIJHIQSPQPSUJPOPGPSHBOJDTVCTUBODFTPSXB

UFSJOTPMVCMFTBNQMFTEFDPNQPTJUJPOVTJOHDPODFOUSBUFEBDJET

BOEPUIFSBHFOUTJTJOEJTQFOTJCMF$PSSFTQPOEJOHFYBNQMFTGSPN

UIFDPMMFDUJPOPGBQQMJDBUJPOTGPSSFBMTBNQMFTBSFBWBJMBCMFPO

SFRVFTU

5IFOFDFTTJUZGPSEFDPNQPTJUJPODBOCFDIFDLFEBDDPSEJOHUPUIF

GPMMPXJOHEJBHSBN

'PSXBTUFXBUFSXJUIBDPOTJTUFOUDPNQPTJUJPOUIJTDIFDLBTB

SVMFOFFECFDBSSJFEPVUPOMZPODF*UJTIPXFWFSBEWJTBCMFUP

DIFDLUIFSFTVMUQFSJPEJDBMMZ



7FSTJPO֮































1PTJUJWFEJTQMBDFNFOUQJQFUUFTQFSNJU

ֽ BOFYBDUEPTBHFPGUIFTBNQMFWPMVNF

ֽ BQSFDJTFNFBTVSFNFOUPGTBNQMFBOESFBHFOUWPMVNFTBOE

PGUIFWPMVNFTPGXBUFSGPSEJMVUJPOQVSQPTFT

1JQFUUFTPGWBSZJOHWPMVNFTBOEBMTPPOFTXJUIBꯎYFEWPMVNFBSF

BWBJMBCMF

4PVSDFTPGFSSPSBOEIJOUTPOIPXUPBWPJEUIFN

$MPTFMZGPMMPXUIFJOTUSVDUJPOTGPSVTFDPOUBJOFEXJUIUIFQJQFUUF

JORVFTUJPO

ֽ $IFDLUIFQJQFUUFEWPMVNFT

B

CZXFJHIJOHVTJOHBOBMZUJDBMTDBMFTXFJHIJOHBDDVSBDZ

q NH NMPGXBUFSBUp$Hq NH

CVTJOH4QFDUSPRVBOUn1JQF$IFDLUIJTJTBQIPUPNFUSJDDIFDL

PGUIFQJQFUUFBOETDBMFTBSFOPUOFDFTTBSZTFF4FDUJPO

ֽ 

"WPJEBODFPGTQSFBEFGGFDUTCZSJOTJOHUIFQJQFUUFTFWFSBM

UJNFTXJUIUIFTPMVUJPOUPCFQJQFUUFE

ֽ "MXBZTFYDIBOHFUIFQJQFUUFUJQ

ֽ %SBXVQUIFMJRVJETMPXMZBOEEFQSFTTQJTUPODPNQMFUFMZUP

EJTDIBSHFUIFMJRVJE

1JQFUUJOH4ZTUFN

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