Merck Milliflex Oasis User manual

SigmaAldrich.com
User Guide
Milliflex Oasis®pump
for the EZ-Fit®filtration unit
The life science business of
Merck operates as MilliporeSigma
in the US and Canada.

Page 2 of 24
Notice
The information provided in this document (or any other literature) is subject to change without prior notice
and shall not constitute a commitment of Merck KGaA, Darmstadt, Germany or any of it’s Aliates. Merck
KGaA, Darmstadt, Germany or any of it’s Aliates expressly decline all warranties and shall not assume any
responsibility for any errors that may appear in this document. This manual is believed to be complete and
accurate at the time of publication. In no event shall Merck KGaA, Darmstadt, Germany or any of its Aliates be
liable for all damages (including but not limited to incidental or consequential damages) in connection with, or
arising from, the use of this document or associated literature.
Table of Contents
Introduction 3
1. Components 4
1.1 Required items 4
1.2 Description of items 5
1.3 Touch switch 6
2. Hardware installation 7
3. Testing 9
3.1 Filtration units 9
3.2 Media plates 9
3.3 Testing 9
3.4 Waste 10
4. Cleaning & maintenance 11
4.1 Cleaning & maintenance table 11
4.2 Surface sanitizers and internal ow
sanitizers table 11
4.3 Filtration head cleaning 12
4.4 Pump surface cleaning 12
4.5 Pump rinsing 12
4.6 Filtration head external gasket cleaning 12
4.7 Pump internal ow sanitization 13
4.8 Filtration head complete cleaning 14
4.9 Filtration head gaskets and check valve
(spare parts) replacement 15
4.10 Sanitization kit gaskets and valve
(spare parts) replacement 16
5. Performance verication 17
6. Use of multiple pumps 18
6.1 Material needed 18
6.1.1 Two pumps connected 18
6.1.2 Three pumps connected 18
6.2 Installation 18
7. System specications 20
8. Ordering information 21
9. Troubleshooting 23
10. Operator & equipment safety instructions 23
11. Technical assistance 23

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Introduction
The Milliex Oasis®pump for the EZ-Fit® ltration unit is a solution for the microbial testing
of aqueous uids.
The system is composed of the Milliex Oasis®pump, the EZ-Fit® ltration unit and culture media.
Please refer to the ordering information section for details on the dierent items.
EZ-Fit® ltration unit:
The EZ-Fit® ltration unit is a disposable ltration device for bioburden testing of liquid
samples including water, in-process samples, and nal products.
The EZ-Fit® ltration units have been designed to ensure optimal microbial recovery. The
sterilized, disposable ltration devices for bioburden testing streamline your workow,
save time and give you highly reliable microbial enumeration results. The blue and pink
drain support and the funnel-to-base assembly have been optimized so that the membrane
remains perfectly at after the sample ltration so that when transferred to the culture
medium, the whole ltration surface of the membrane is in perfect contact with the culture
media. The shape of the funnel and the hydrophobic material minimize sample residue
ensuring that the complete sample volume reaches the membrane surface. The innovative
funnel assembly design prevents any leaks or sample by-pass of the membrane, meaning
that the whole sample volume is ltered. EZ-Fit® ltration units conform to international
standards (EP/USP) and comply with water testing regulations.

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1. Components
1.1 Required items
Three hardware item numbers are necessary to run the system:
1. Milliex Oasis®pump for EZ-Fit® ltration unit (item MMSYSTEZ1)
2. Milliex Oasis® power supply for one to three ltration pumps, specic to
dened regions:
• Australia: item MMPWRSPAU
• Brazil: item MMPWRSPBR
• China: item MMPWRSPCN
• Denmark: item MMPWRSPDK
• Europe: item MMPWRSPEU
• Switzerland: item MMPWRSPSZ
• India: item MMPWRSPIN
• UK: item MMPWRSPUK
• Japan: item MMPWRSPJP
• South Africa: item MMPWRSPZA
3. Milliex Oasis® internal ow sanitization kit (item MMSANKIT1)
Optional: For higher throughput capability, it is possible to connect two or
three pumps together without additional power supplies. In this case, the
Milliex Oasis®electrical cable for connecting two pumps (item MMCABLEMM)
is required per additional pump.
Consumables: please refer to the ordering information section for details on
the dierent items.
1. Filtration units
2. Culture media
3. Consumables for the Milliex Oasis® internal ow sanitization kit

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1.2 Item description
The Milliex Oasis®pump for
EZ-Fit® ltration unit package
(item MMSYSTEZ1) contains
1. Pump body
2. Two ltration heads with their covers
3. Tubing for discarding the liquids
4. Accessory kit
5. Spare parts kit
The Milliex Oasis®power supply
package contains
1. Power supply
2. Power cable (region specic)
3. Two rubber fasteners
The Milliex Oasis® internal ow
sanitization kit (item MMSANKIT1)
package contains:
1. Stainless steel sanitization kit with
plug and gasket removal tool
2. Vacuum gauge
The EZ-Fit® ltration unit package
contains 48 units.
Culture media: please refer to the
ordering information section to
select your preferred media.
The Milliex Oasis®consumable for
internal ow sanitization kit (item
MMSANSYFU) package contains:
1. Twenty-four plastic syringes for
dispensing the sanitizer
2. Twenty-four funnel assemblies for
rinsing with puried water
Figure 1: Milliex Oasis®pump for EZ-Fit® ltration
unit (MMSYSTEZ1) package components
Figure 2: Milliex Oasis® power supply package
components
Figure 3: Milliex Oasis® internal ow sanitization
kit package components

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1.3 Touch switch
Color Meaning
White Steady: pump is ready for next handling step
Blinking: membrane dry out on-going
Blue Vacuum on
Green The sanitization kit is ready to use
Red No ltration head or ltration head is not locked
Orange Blinking: synchronizing multiple pumps, wait
O No tension or standby
The switch is touch-sensitive. Use your
ngertip to activate it.
Touch the center of the switch without
touching the outer ring.
When the Milliex Oasis®pump is in standby
mode, it can be reactivated by touching the
space between the two switches.
Figure 4: INCORRECT—no activation if the nger
touches the outer ring
Figure 5: CORRECT nger position to start or stop
the pump
✓
✗
Note: If unused for thirty minutes, the
pump switches o. Touch the switch
once to power up.

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2. Hardware installation
The pump must be installed on a horizontal
surface. Please make sure that your working
area is level to ensure good sample volume
readings.
• Take the pump body, pump heads and
tubing out of the package with care
• Take out the accessory bag and spare
part bag
Optional: the accessory bag contains four
stickers; select your preferred sticker and
stick it carefully into the specific groove on
the pump.
Figure 6: Pump sticker set up
• Clean the exterior of the pump and the
tubing using a wipe moistened with a
surface sanitizer listed in the cleaning &
maintenance section
• Place the pump in the working area
Under a horizontal laminar flow hood (LFH),
the front of the pump should be placed at
least at 160 mm (6.3 in.) from the front
edge of the LFH (see picture).
Figure 7: The front of the pump should be placed
at least at 160 mm (6.3 in.) from the front edge of
a horizontal LFH
• On the back of the pump, press the metal
latch and remove the white plug, then
connect the tubing
Figure 8: The metal latch must be pressed to
remove the white plug
• Place the other end of the tubing in a
liquid waste container
• Take out the power supply out of its
package
• Sanitize the power supply in the same
way as the pump
• Plug the DC power cord to one power port
situated at the back of the pump. Push
the connectors into the power port until
a click is heard
• Connect the free power cord to the
power supply
• Connect the other end of the power cord
to a properly grounded power outlet
Optional: attach the power supply outside
the working area with the two rubber
fasteners.
Figure 9: The power supply can be attached
outside the working area with the rubbers
fasteners

Page 8 of 24
Optional: to arrange the tubing and
electrical cable in your working area, use
the Milliflex Oasis®guides for electrical
cable and tubing (item MMTUGUIDE).
Figure 10: Use of Milliflex Oasis®guides for
electrical cable and tubing (item MMTUGUIDE)
• Sanitize the internal flow of the pump
referring to the cleaning & maintenance
section
• Once the internal flow path sanitization
has been performed, take the two pump
heads out of their package and remove
the yellow covers
• Clean the pump heads using a wipe
moistened with a surface sanitizer listed
in the cleaning & maintenance section
• Install the pump heads on the pump body
• Lock the pump heads by turning
clockwise until the sign on the head is
aligned with the one on the pump body
Figure 11: The sign on a locked head is aligned
with the one on the pump body
The pump is ready for testing.

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3. Testing
The following components are needed
before starting your sample processing:
• Milliflex Oasis®pump for EZ-Fit®filtration
unit installed following the installation
section
• EZ-Fit®filtration units
• Culture media
• Samples to test
Ensure that the internal pump flow was
sanitized according to the cleaning &
maintenance section.
• Clean the exterior of the pump using a
wipe moistened with a surface sanitizer
listed in the cleaning & maintenance
section
3.1 Filtration units
Different types of filtration units are
available (see ordering information section)
Note: for testing samples with
antimicrobial activity, select filtration
units with low binding Durapore®
membrane.
Note: Isopropyl myristate (IPM) is not
chemically compatible, therefore sample
containing IPM should not be used.
• Open a box of filtration units and remove
the desired numbers of bags or filtration
units
• Repeat the operation if more than one
box of filtration units is needed.
• Clean each bag or each unit using a wipe
moistened with a surface sanitizer
3.2 Media plates
Follow the instructions of use for your
selected culture media
3.3 Testing
When the pump is on standby mode,
the switch is not lit up. To power up the
Milliex Oasis®pump, touch the front of the
pump between the two switches with your
ngertip.
Note: If starting a filtration without a
filtration unit placed on the filtration
head, you will hear a whistle noise, this
is due to a check valve to be found in
each pump head and is perfectly normal.
If the pump is started after having
placed a filtration unit on the pump head,
this noise will not be heard.
• Remove the device from the packaging by
grasping the funnel
Figure 12: Removing the unit from its packaging
• Remove the plug from the bottom of
the base (blue unit bulk packaging
versions only)
Figure 13: Removing the plug from the unit
(blue unit bulk packaging versions only)

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• Place the EZ-Fit®filtration unit on the
pump head
Figure 14: Filtration unit placement on the
pump head
• Remove the funnel’s cover and pour
the sample into the unit
• Place the cover back in place
• Touch the switch to start the filtration
Note: touch the switch three times within
one second to launch the ltration on
two ltration heads simultaneously.
• Open a culture media plate.
• When the liquid is totally filtered, touch
the switch once more to stop the filtration
• After a few seconds, the pump stops,
and the switch light stops blinking then
remove the cover and slightly pinch the
funnel and remove it as shown here:
Figure 15: Funnel removal
• Remove the membrane using forceps.
Figure 16: Membrane removal
• Place the membrane onto the culture
media plate, close the plate, it is ready
for incubation
Figure 17: Membrane transfer onto culture media
• Remove the base from the filtration head
as shown here:
Figure 18: Filtration unit base removal
Note: If some ltered liquid remains in
the pump head, it is possible to remove
it with a dry wipe. Regardless of the
amount of liquid, it does not impact the
test results.
3.4 Waste
After usage, the funnels can be stacked to
reduce waste volume.
Please refer to the waste management
document available on our website to
recycle the materials.

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4. Cleaning & Maintenance
4.1 Cleaning & Maintenance table
Component Action
First
installation
Before each
testing shift
After each
testing shift Monthly Yearly
Filtration head Cleaning x x x
Pump Surface cleaning xxx
Pump Internal ow
sanitization x x
Pump Rinsing If testing non-
water samples
Filtration head External gasket
cleaning
If testing non-
water samples
Filtration head Complete
cleaning x
Filtration head Spare parts
replacement x
Sanitization kit Spare parts
replacement x
Note: Even if not mandatory, the filtration head including its components can be
autoclaved at 121 °C for 15 minutes or at 134 °C for 5 minutes.
4.2 Surface sanitizers and internal flow sanitizers tables
Surface sanitizer tested: Active ingredients Suppliers Dilution
Ethanol (N°CAS 64-17-5 : 226 mg/g)
Chlorhexidine Digluconate (N°CAS 18472-51-0 : 0.64 mg/g)
Didecyldimethylammonium chloride (N°CAS 7173-51-5 : 0.53 mg/g), Excipients.
Consult our local
technical support Ready to use
Propan-2-ol (70%), deionised water solution (30%) Consult our local
technical support Ready to use
Ethanol (70%) N/A Ready to use
Quaternary ammonium: Benzalkonium chloride (between 0.25–0.5%)
Poly (hexamethylene biguanide) hydrochloride (between 0.1–0.25%)
Consult our local
technical support Ready to use
Sodium Hypochlorite (2.6% active chloride) N/A 250 ppm
hydrogen peroxide (6%) Consult our local
technical support Ready to use
Isopropyl alcohol (N°CAS 67-63-0 10%)
Didecyldimethylammonium chloride (N°CAS 7173-51-5: 0.15936%)
Consult our local
technical support Ready to use
hydrogen peroxide (1%) and acetic acid (5.2%) Consult our local
technical support Ready to use
Peracetic acid (0.08%), hydrogen peroxide (1%), and acetic acid (<10%) Consult our local
technical support Ready to use
Quaternary ammonium: Ethanol<25%, Didecyldimethylammonium chloride <0.5%
N-(3-aminopropyl)-N-dodecylpropane-1,3diamine <0.5%
Consult our local
technical support Ready to use
Phenolic Disinfectant: Biphenyl-2-ol (CAS No 90-43-7: 7.7%)
clorophene (CAS No 120-32-1: 7.7%)
Consult our local
technical support 8 mL/L
Alkaline disinfectant: Potassium hydroxide (CAS 1310-58-3)
Tetrasodium ethylenediaminetetraacetate (CAS 64-02-8)
Consult our local
technical support 47 mL/L

Page 12 of 24
Internal flow sanitizers: active ingredientsInternal flow sanitizers: active ingredients SuppliersSuppliers DilutionDilution
Quaternary ammonium: Benzalkonium chloride (between 0.25–0.5%)
Poly (hexamethylene biguanide) hydrochloride (between 0.1–0.25%)
Consult our local
technical service Ready to use
Sodium Hypochlorite (2.6% active chloride) N/A 250 ppm
hydrogen peroxide (6%) Consult our local
technical support Ready to use
Isopropyl alcohol (N°CAS 67-63-0 10%)
Didecyldimethylammonium chloride (N°CAS 7173-51-5: 0.15936%)
Consult our local
technical support Ready to use
Note: Sanitizers containing acetic acid at 0.1% or higher should not be used for
internal flow sanitization because of chemical incompatibility.
4.3 Filtration head
cleaning
Remove the filtration heads and clean
them using a wipe moistened with a
surface sanitizer listed in the cleaning &
maintenance section, then put them back
in place.
4.4 Pump surface cleaning
Clean the exterior of the pump, the tubing
and the cable using a wipe moistened a
surface sanitizer listed in the cleaning &
maintenance section.
4.5 Pump rinsing
If samples other than water have been
processed, filter 300 mL of purified water at
the end of each day. This is recommended
to ensure the functionality of internal parts.
Use either EZ-Fit®Filtration Unit or
the internal flow sanitization kit with a
sanitization funnel assembly to perform
the rinsing.
4.6 Filtration head external
gasket cleaning
• If testing non-water samples, remove the
external gasket, then clean it including its
groove
Figure 19: External gasket removal

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4.7 Pump internal flow
sanitization
Material needed:
1. Sanitization kit (item MMSANKIT1)
2. Consumables for sanitization kit
(item MMSANSYFU)
3. Sanitizer (see table)
4. Purified water
• Remove the pump heads
• Place a sanitization kit on one pump head
support and lock it
• Lock the sanitization kit plug on the
other support
Optional: For faster sanitization, it is
possible to replace the plug by a second
sanitization kit and sanitize the complete
pump at once.
• Rotate the trigger counter clockwise,
the switch turns green
Figure 20: Trigger is rotated counter clockwise
• Take one sanitization funnel assembly
(sanitization kit consumable) and
remove its support, then place the
funnel on the kit
• Take a syringe (sanitization kit
consumable) and remove its plunger
• Place and lock the syringe on the kit
• Pour 60 mL of sanitizer in the syringe
• Introduce the plunger into the syringe
Note: An air pocket is visible over the
liquid.
Figure 21: Visible air pocket over the liquid in the
sanitization syringe
• Push the plunger to bring the liquid level
to the 50 mL level indicator
Figure 22: Plunger pushed to bring the liquid
to the 50 mL level indication of the sanitization
syringe
• Open the funnel lid
• Pour puried water up to the 250 mL
indication level
• Put back the funnel lid
• Touch the switch to start the pump
Note: the switch starts blinking for
15 minutes while the following
operations occur:
1. The plunger is partially pulled down
and the sanitizer fills the pump internal
flow.
2. About 10 mL of sanitizer remains into
the syringe
3. The sanitizer remains in contact with
the internal flow for 15 minutes

Page 14 of 24
4. The plunger is pulled down completely
5. The water in the funnel is pulled to rinse
the pump
6. The switch stops blinking
7. The pump switches off if not in use for
thirty minutes
• When the light is steady or if the pump
is switched off, repeat the operation for
the second flow path
• Discard the sanitization kit consumables
• Remove the sanitization kit and clean
it using a wipe moistened with a
surface sanitizer listed in the cleaning &
maintenance section
Note: the sanitization kit can be auto-
claved at 121 °C for 15 min or at 134 °C
for 5 min.
4.8 Filtration head
complete cleaning
Materials:
1. Pump head
2. Gasket removal tool (to be found in
the pump spare part kit)
3. Sanitizer (see table)
• Remove the filtration head from the pump
• Remove the external silicone gasket at
the exterior of the head
Figure 23: External gasket removal
• Remove the black check valve with the
metal tool provided in the gasket kit
Figure 24: Check valve removal with tool—part 1
Figure 25: Check valve removal with tool—part 2
• Remove the internal gasket using the
gasket removal tool
• Clean the stainless-steel pump head
carefully including the grooves using a wipe
moistened with a surface sanitizer listed
in the cleaning & maintenance section

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• Clean the gaskets and the check valve
carefully using a wipe moistened with
the sanitizer
• Put the gaskets and check valve back in
place using the gasket removal tool
Figure 26: Check valve installation with tool—part 1
Figure 27: Check valve installation with tool—part 2
4.9 Filtration head gaskets
and check valve (spare
parts) replacement
Materials:
1. Pump head
2. Spare part kit to be found in the pump
package or in the “filtration head for
EZ-Fit®filtration unit gasket kit” item
MMGASKTEZ
Figure 28: Filtration head for EZ-Fit®filtration
unit gasket kit, item MMGASKTEZ
• Remove the filtration head
• Remove the gaskets and the check
valve and discard them (details in
previous chapter)
• Take out a set of gaskets and one
(black) check valve out of the gasket
kit bag and clean them using a wipe
moistened with a sanitizer
• Put the new gaskets and the check
valve in place using the gasket removal
tool (details in previous chapter)

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4.10 Sanitization kit
gaskets (spare parts)
replacement
Materials:
1. Sanitization kit (item MMSANKIT1)
2. Sanitization kit gasket kit (item
MMGASKTSK) containing:
• Five O-rings for securing funnel to
kit tightness
• Five O-rings for securing kit to pump
tightness
• Remove the gaskets (using the metal
tool provided with the gasket kit) from
the sanitization kit and discard them
• Take out a set of gaskets out of the
gasket kit bag and clean them using a
wipe moistened with a surface sanitizer
listed in the cleaning & maintenance
section
• Put the new gaskets in place

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5. Performance verification
The accuracy of the vacuum level provided
by the pump can be easily checked at
any time, however this verification is
not mandatory.
Materials:
1. Sanitization kit (item MMSANKIT1)
2. Consumables for sanitization kit
(item MMSANSYFU)
3. Milliflex Oasis®vacuum gauge for pump
verification testing (item MMSANSYFU)
• This item is provided in the sani-
tization kit or can be purchased
separately (it comes with a certif-
icate of calibration).
4. Stopwatch
5. Purified water
• Remove the pump heads
• Place the sanitization kit and lock it on
the pump head support Lock the sani-
tization kit plug on the other support
• Rotate the trigger clockwise, the switch
turns white
• Place a sanitization funnel assembly
(sanitization kit consumables) on the
sanitization kit
• Connect the Milliflex Oasis®vacuum
gauge to the syringe port of the kit
Figure 29: Set up for performance verification
Internal flow prewetting step:
1. Pour purified water up to the 250 mL
indication level
2. Touch the switch to start the filtration
3. Stop the filtration when it is complete
4. Disconnect the vacuum gauge to reset
it and connect it back to the syringe
port of the kit
Verification of the vacuum level and
verification of the flow time:
5. Pour again purified water in the funnel
up to the 250 mL indication level
6. Start the stop watch and at the same
time the filtration by touching the switch
7. Record the vacuum level on the gauge
and the time needed to complete the
filtration
8. Stop the filtration when there is no
more water
9. Disconnect the vacuum gauge to reset
it and connect it back to the syringe
port of the kit
10. Repeat steps 4 to 9 twice more
Check that the average values are within
the following specifications.
Pressure Filtration time
< –0.4 bars ≤ 25 seconds for 250 mL water
• Remove the sanitization kit and clean
it using a wipe moistened with a
surface sanitizer listed in the cleaning &
maintenance section

Page 18 of 24
6. Use of multiple pumps
Up to three pumps can be connected
providing the following benefits:
• Higher throughput
• Use of single power supply
• Daisy chain start: up to six samples
filtered with a single push of a button
6.1 Material needed
6.1.1 Two pumps connected:
• Two Milliflex Oasis®pumps for EZ-Fit®
filtration unit (item MMSYSTEZ1)
• Milliflex Oasis®electrical cable
for connecting two pumps
(item MMCABLEMM)
• Milliflex Oasis®power supply, the item
number is specific to defined regions:
- Australia: item MMPWRSPAU
- Brazil: item MMPWRSPBR
- China: item MMPWRSPCN
- Denmark: item MMPWRSPDK
- Europe: item MMPWRSPEU
- Switzerland: item MMPWRSPSZ
- India: item MMPWRSPIN
- UK: item MMPWRSPUK
- Japan: item MMPWRSPJP
- South Africa: item MMPWRSPZA
Optional: Milliflex Oasis®T connectors for
tubing (item MMTCNNECT)
6.1.2 Three pumps connected:
• Three Milliflex Oasis®pumps for EZ-Fit®
filtration unit (item MMSYSTEZ1)
• Two Milliflex Oasis®electrical cables
for connecting two pumps (item
MMCABLEMM)
• Milliflex Oasis®power supply, the item
number is specific to defined regions
Optional: Milliflex Oasis®T connectors for
tubing (item MMTCNNECT)
6.2 Installation
The pumps must be installed on a level
surface to ensure good sample volume
readings.
• Take the pump bodies and the tubing
out of the package with care
• Take out the accessory bags
Optional: the accessory bag contains four
stickers, select your preferred sticker and
stick it carefully to the specific groove on
each pump.
Figure 30: Pump sticker set up
• Clean the exterior of the pumps and
the tubing using a wipe moistened with
a surface sanitizer listed in the cleaning
& maintenance section
• Place the pumps in the working area
Under a horizontal laminar flow hood, the
front of the pumps must be placed at least
at 160 mm (6.3 in.) from the front edge of
the LFH (see picture).
Figure 31: The front of the pump must be placed
at least at 160 mm (6.3 in.) from the front edge
of a horizontal LFH

Page 19 of 24
• At the back each pump, press the metal
latch and remove the white plug, then
connect the tubing
• Place the other end of the tubing in a
liquid waste container
Optional: to save space, it is possible
to connect the tubing together using
the Milliflex Oasis®T connectors (item
MMTCNNECT). In this case cut the tubing
to the desired length and connect them.
• Take out the power supply out of its
package
• Clean the power supply as described
previously
• Use the Milliflex Oasis®electrical cable for
connecting two pumps (item MMCABLEMM)
to connect one power port of pump A to
one power port of pump B. Repeat for the
third pump if applicable
• Push the connectors into the power ports
until a click is heard
• Plug the DC power cord in the free power
port, at the back of the Milliflex Oasis®
pump A
• Plug the region-specific power cord to the
appropriate electrical power source; the
Milliflex Oasis® pumps power-up: all the
touch switches light up
Optional: to arrange the tubing and the
electrical cable in your working area,
it is possible to use the Milliflex Oasis®
guides for electrical cable and tubing
(item MMTUGUIDE)
Optional: attach the power supply outside
the working area with the two rubber
fasteners
Figure 32: The power supply can be attached
outside the working area with the rubber fasteners
• For each pump, remove the two pump
heads from their package and remove
the yellow covers
Optional: From the accessory bags, select
colored rings, clean them using a wipe
and one of the sanitizers recommended in
the cleaning & maintenance section, then
install them on the pump heads.
• Clean the pump heads using a wipe
moistened with a surface sanitizer listed
in the cleaning & maintenance section
• Install the pump heads on the pump
bodies
• Lock the pump heads by turning
clockwise until the sign on the head is
aligned with the one on the pump body
Figure 33: The sign on a locked head is aligned
with the one on the pump body
• Sanitize the internal flow path of the
pumps, please refer to the sanitization
described in the cleaning & maintenance
section
The pumps are ready for testing.
To start multiple filtrations simultaneously
(daisy chain), touch the switch three times
within one second. Please refer to the
testing section of this manual for the other
steps of the testing.

Page 20 of 24
7. System specifications
Milliflex Oasis®pump
Frame Acrylonitrile Styrene Acrylate and Polycarbonate, compatible with standard UV sterilization
Filtration heads Two per pump, stainless steel, silicone, rubber gaskets
Can be autoclaved at 121 °C for 15 minutes or at 134 °C for 5 minutes
Pump dimensions
Height 90 mm (3.5 in.)
Width 230 mm (9.0 in.)
Depth 310 mm (12.2 in.)
Filtration support height 75 mm (2.8 in.)
Weight with filtration heads 3.4 kg (7.5 lb)
Power supply 100V–240V 50/60Hz—One set can be used for up to three pumps
EZ-Fit®Filtration Unit
Material of construction
Cover Polystyrene
Funnel Styrene butadiene copolymer (SBC)
Membrane Mixed cellulose esters, PVDF
Support pad (blue units only) Cellulose
Base Acrylonitrile butadiene styrene
Plug (Bulk blue units only) Low-density polyethylene
Dimensions
Height 100 mL: 66.5 mm (2.6 in.)
250 mL: 108.5 mm (4.3 in.)
Largest diameter 75.8 mm (3.0 in.)
Filtration surface 12.56 cm²
Sterilization method EO for blue units, e-beam irradiation for pink units
Milliflex Oasis® pump: materials in contact with the liquid filtered
Part Material
Filtration head Stainless steel ASI 316 L
Silicone (gaskets & check valve)
Inner tubing Low-density polyethylene (LDPE)
Tubing fittings Polyphenylsulfone (PPSU)
Polypropylene (PP)
Acetal
Buna N rubber
Core pump module Polypropylene (PP)
FKM (Viton®)
PTFE (Teflon®)
Drain tubing Silicone