MGI Complete Genomics DNBSEQ-G800RS User manual

For Research Use Only.
Complete Genomics, Inc.
DNBSEQ-G800RS
User Manual

About this manual
This manual is applicable to Genetic Sequencer (DNBSEQ-G800RS). The manual
version is 1.0 and the software version is V1.
This manual and the information contained herein are proprietary to Complete
Genomics, Inc. (hereinafter referred to as CG), and are intended solely for the
contractual use of its customers in the use of the products described herein
and for no other purpose. This manual and its content shall not be reprinted,
reproduced, modified, distributed, or disclosed to others, in whole or in part,
without prior written consent from CG.
CG makes no commitment to this manual, including (but not limited to) any
special purpose commercial and any reasonable implied warranties. CG has
taken measures to ensure the correctness of this manual. However, CG is not
responsible for any missing parts of the manual, and reserves the right to revise
the manual and modify the device, so as to improve the reliability, performance
or design.
Figures in this manual are for illustrative purpose only. The content might be
slightly dierent from the device. For the most up to date details, refer to the
device purchased.
DNBSEQTM is a trademark of CG or its affiliates in U.S. and/or other
countries. Intel® and Xeon® are trademarks of Intel Corporation or its
subsidiaries in the U.S. and/or other countries. QubitTM is the trademark of
Thermo Fisher Scientific, or its subsidiaries. ProClinTM is a trademark of the
Dow Chemical Company (Dow) or an aliated company of Dow. Other names
and trademarks mentioned in this manual are the property of their respective
companies and subsidiaries.
©2023 Complete Genomics, Inc. All rights reserved.

Revision history
Date Version
Initial release March 15,2023 1.0

Contents
I
Contents
Safety 1
Conventions used in this guide 2
General safety 2
Electrical safety 4
Mechanical safety 4
Components safety 4
Biological safety 5
Symbols 5
Packaging 5
Device 6
Label 7
User manual 8
Device overview 9
Intended use 10
Working principle 10
Sequencer overview 11
Structural composition 11
Basic components 12
Control software 20
Laboratory requirements 27
Site requirements 28
Network requirements 29
Device requirements 30
Power supply requirements 31
Peripheral device requirements 32
Sequencing 33

Contents Manual
II
Workflow 34
Preparing DNBs 34
Preparing the reagent cartridge and flow cell 35
Reagent cartridge and sequencing flow cell 35
Cleaning cartridge and washing flow cell 35
Preparing the device 35
Powering the device on 35
Logging in to the control software 36
Performing pre-run checks 36
Performing an auto wash 37
Loading the flow cell 37
Cleaning the flow cell satge 37
Loding new flow cell 37
Loading the sequencing reagent cartridge 39
Preparing the reagent cartridge compartment 39
Loding the reagent cartridge 40
Loading DNBs 40
Reviewing parameters 42
Performing sequencing run 42
Performing a regular wash 44
Disposing of the sequencing reagent cartridge and
flow cell 44
(Optional) Powering the device o 45
Device maintenance 47
Service plan 48
Daily maintenance 48
Maintaining the power cord 48
Checking and cleaning the cooling fan 48

Contents Manual
III
Flow cell stage and flow cell maintenance 49
Introduction of flow cell stage and flow cell 49
Cleaning the flow cell stage and flow cell 50
Cleaning sealing gasket 51
Sequencer maintenance 51
Wash 51
Weekly maintenance 56
Monthly maintenance 57
Annual maintenance 58
Maintaining the software 58
FAQs 58
Storage and transportation 60
Disposal of the device 60
Device specifications 61
Compliance information 63
Manufacturer information 65
Order information 67
Acronyms and abbreviations 69
Index 71

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1
01
Safety
This chapter describes basic safety information about the device.
Carefully read and understand the information before use to ensure
correct operations, best performance, and personnel safety. Keep this
guide at hand for reference at any time.

Safety Conventions used in this guide
2
Conventions used in this guide
The following table describes conventions that are used in this guide:
Item Description
shall Means compliance with a requirement or it is mandatory for
compliance with this document
should Means compliance with a requirement but it is not mandatory for
compliance with this document
may Used to describe possibility or probability
can Used to describe permission and capability
must Used to express a constraint
Boldface Indicates the printings and on-screen characters on the device
General safety
DANGER
yEnsure that the device is operated under the conditions specified in this guide. Otherwise,
it may cause altered experimental results, device malfunction, or even personal injury.
yEnsure that the components of the device are completely installed before operation.
Otherwise, it may cause in personal injury.
yA laser is installed in the device. Laser radiation can cause eye injury and skin burns.
Before performing a sequencing run, ensure that the flow cell compartment door of the
device is closed. Use of controls or adjustments or performance of procedures other than
those specified herein may result in hazardous radiation exposure.
yMaintain the device by following the instructions described in this manual to ensure best
performance. Otherwise, it might result in device malfunction or even personal injury.
yDo not operate the device in the presence of flammable or explosive liquids, vapors, or
gases. Otherwise, it might result in device damage, or even personal injury.
yDo not operate the device during maintenance or transportation.

Safety General safety
3
WARNING
yOnly CG Technical Support or qualified and trained personnel can unpack, install, move,
debug and maintain the device. Incorrect operations can cause altered experimental
results or damage to the device.
yDo not move the device after CG Technical Support have installed and debugged the
device. Unauthorized moves of the device can cause altered experimental results. If you
require to move the device, contact CG Technical Support.
yOnly trained personnel can operate the device.
yDo not disconnect the power cord when the device is on. Otherwise, it may result in
device malfunction.
yOnly the components provided by the manufacturer can be used for device maintenance.
Unapproved components may degrade device performance or result in device
malfunction.
yDo not reuse disposable items, except where noted in this manual.
yDo not place tubes or reagent kits on the device. Liquids seeping into the device may
damage it.
CAUTION
yOnly the peripheral devices and consumables specified by the manufacturer can be used.
yIf you have maintenance questions that are not mentioned in this manual, contact CG
Technical Support.
yThe device has been inspected and validated before delivery. If serious deviation occurs
during use, contact CG Technical Support for troubleshooting and calibration.
yEnsure that you are familiar with the operation of all the laboratory apparatus to be used.
yThis sequencing reagent kit is for one sequencing run only and cannot be reused.
yThe components and packages are batched separately. Keep the components in the
packages until use and do not remove them. Mixed use of reagent components from
dierent batches of kits is not recommended.