MGI Complete Genomics DNBSEQ-G800RS User manual

For Research Use Only.
Complete Genomics, Inc.
DNBSEQ-G800RS
User Manual

About this manual
This manual is applicable to Genetic Sequencer (DNBSEQ-G800RS). The manual
version is 1.0 and the software version is V1.
This manual and the information contained herein are proprietary to Complete
Genomics, Inc. (hereinafter referred to as CG), and are intended solely for the
contractual use of its customers in the use of the products described herein
and for no other purpose. This manual and its content shall not be reprinted,
reproduced, modified, distributed, or disclosed to others, in whole or in part,
without prior written consent from CG.
CG makes no commitment to this manual, including (but not limited to) any
special purpose commercial and any reasonable implied warranties. CG has
taken measures to ensure the correctness of this manual. However, CG is not
responsible for any missing parts of the manual, and reserves the right to revise
the manual and modify the device, so as to improve the reliability, performance
or design.
Figures in this manual are for illustrative purpose only. The content might be
slightly dierent from the device. For the most up to date details, refer to the
device purchased.
DNBSEQTM is a trademark of CG or its affiliates in U.S. and/or other
countries. Intel® and Xeon® are trademarks of Intel Corporation or its
subsidiaries in the U.S. and/or other countries. QubitTM is the trademark of
Thermo Fisher Scientific, or its subsidiaries. ProClinTM is a trademark of the
Dow Chemical Company (Dow) or an aliated company of Dow. Other names
and trademarks mentioned in this manual are the property of their respective
companies and subsidiaries.
©2023 Complete Genomics, Inc. All rights reserved.

Revision history
Date Version
Initial release March 15,2023 1.0

Contents
I
Contents
Safety 1
Conventions used in this guide 2
General safety 2
Electrical safety 4
Mechanical safety 4
Components safety 4
Biological safety 5
Symbols 5
Packaging 5
Device 6
Label 7
User manual 8
Device overview 9
Intended use 10
Working principle 10
Sequencer overview 11
Structural composition 11
Basic components 12
Control software 20
Laboratory requirements 27
Site requirements 28
Network requirements 29
Device requirements 30
Power supply requirements 31
Peripheral device requirements 32
Sequencing 33

Contents Manual
II
Workflow 34
Preparing DNBs 34
Preparing the reagent cartridge and flow cell 35
Reagent cartridge and sequencing flow cell 35
Cleaning cartridge and washing flow cell 35
Preparing the device 35
Powering the device on 35
Logging in to the control software 36
Performing pre-run checks 36
Performing an auto wash 37
Loading the flow cell 37
Cleaning the flow cell satge 37
Loding new flow cell 37
Loading the sequencing reagent cartridge 39
Preparing the reagent cartridge compartment 39
Loding the reagent cartridge 40
Loading DNBs 40
Reviewing parameters 42
Performing sequencing run 42
Performing a regular wash 44
Disposing of the sequencing reagent cartridge and
flow cell 44
(Optional) Powering the device o 45
Device maintenance 47
Service plan 48
Daily maintenance 48
Maintaining the power cord 48
Checking and cleaning the cooling fan 48

Contents Manual
III
Flow cell stage and flow cell maintenance 49
Introduction of flow cell stage and flow cell 49
Cleaning the flow cell stage and flow cell 50
Cleaning sealing gasket 51
Sequencer maintenance 51
Wash 51
Weekly maintenance 56
Monthly maintenance 57
Annual maintenance 58
Maintaining the software 58
FAQs 58
Storage and transportation 60
Disposal of the device 60
Device specifications 61
Compliance information 63
Manufacturer information 65
Order information 67
Acronyms and abbreviations 69
Index 71

---This page is intentionally left blank.---

1
01
Safety
This chapter describes basic safety information about the device.
Carefully read and understand the information before use to ensure
correct operations, best performance, and personnel safety. Keep this
guide at hand for reference at any time.

Safety Conventions used in this guide
2
Conventions used in this guide
The following table describes conventions that are used in this guide:
Item Description
shall Means compliance with a requirement or it is mandatory for
compliance with this document
should Means compliance with a requirement but it is not mandatory for
compliance with this document
may Used to describe possibility or probability
can Used to describe permission and capability
must Used to express a constraint
Boldface Indicates the printings and on-screen characters on the device
General safety
DANGER
yEnsure that the device is operated under the conditions specified in this guide. Otherwise,
it may cause altered experimental results, device malfunction, or even personal injury.
yEnsure that the components of the device are completely installed before operation.
Otherwise, it may cause in personal injury.
yA laser is installed in the device. Laser radiation can cause eye injury and skin burns.
Before performing a sequencing run, ensure that the flow cell compartment door of the
device is closed. Use of controls or adjustments or performance of procedures other than
those specified herein may result in hazardous radiation exposure.
yMaintain the device by following the instructions described in this manual to ensure best
performance. Otherwise, it might result in device malfunction or even personal injury.
yDo not operate the device in the presence of flammable or explosive liquids, vapors, or
gases. Otherwise, it might result in device damage, or even personal injury.
yDo not operate the device during maintenance or transportation.

Safety General safety
3
WARNING
yOnly CG Technical Support or qualified and trained personnel can unpack, install, move,
debug and maintain the device. Incorrect operations can cause altered experimental
results or damage to the device.
yDo not move the device after CG Technical Support have installed and debugged the
device. Unauthorized moves of the device can cause altered experimental results. If you
require to move the device, contact CG Technical Support.
yOnly trained personnel can operate the device.
yDo not disconnect the power cord when the device is on. Otherwise, it may result in
device malfunction.
yOnly the components provided by the manufacturer can be used for device maintenance.
Unapproved components may degrade device performance or result in device
malfunction.
yDo not reuse disposable items, except where noted in this manual.
yDo not place tubes or reagent kits on the device. Liquids seeping into the device may
damage it.
CAUTION
yOnly the peripheral devices and consumables specified by the manufacturer can be used.
yIf you have maintenance questions that are not mentioned in this manual, contact CG
Technical Support.
yThe device has been inspected and validated before delivery. If serious deviation occurs
during use, contact CG Technical Support for troubleshooting and calibration.
yEnsure that you are familiar with the operation of all the laboratory apparatus to be used.
yThis sequencing reagent kit is for one sequencing run only and cannot be reused.
yThe components and packages are batched separately. Keep the components in the
packages until use and do not remove them. Mixed use of reagent components from
dierent batches of kits is not recommended.

Safety Electrical safety
4
Electrical safety
DANGER
yEnsure that the device is properly grounded, and the grounding resistance meets the
requirements. Failure to do so may result in altered experimental results, electrical
leakage, or even electrical shock. If you have concerns about proper device grounding,
please contact CG Technical Support.
yDo not remove the device cover and expose the inner components. Otherwise, electrical
shock may be caused.
WARNING
Do not use the device in close proximity to sources of strong electromagnetic fields, such
as unshielded sources of radiated emissions. Radiated signals might reduce the accuracy of
the results.
CAUTION
yBefore initial use of the device, assess the electromagnetic environment in which
the device will be used. The electromagnetic environment should meet Federal
Communications Commission-Part15A. For details, contact CG Technical Support.
yEnsure that the input voltage meets the device requirements.
yEnsure that the voltage of the power outlet in your laboratory or the UPS (uninterruptible
power supply) (if any) meets the voltage requirements before using the device. Failure to
do so might damage the electrical components.
yPrepare the laboratory and power supply according to the instructions described in this
manual.
Mechanical safety
DANGER
To avoid device damage and personal injury, place the device on a level surface that meets
the load-bearing requirements and ensure that the device cannot be easily moved.
Components safety
WARNING
yOnly the software that has been provided by the manufacturer can be installed and used
on the device. Other software may interfere with normal device functions, or even cause
data loss.
yDo not uninstall the control software by yourself. If any problem occurs during software
operation, contact CG Technical Support.
yIf the fuse blew, replace the fuse with the specified type. For details, contact CG Technical
Support.
CAUTION
Ensure that peripheral devices meet the requirements of IEC/EN 62368-1.

Safety Biological safety
5
Biological safety
yReagents and waste chemicals may cause personal injury through skin, eye, or mucosal
contact. Follow the safety standards of your laboratory and wear protective equipment
(such as a laboratory coat, protective glasses, mask, gloves, and shoe covers) when using
the device.
yIf you accidentally splash reagents or waste liquids on your skin or into your eyes,
immediately flush the affected area with large amounts of water and seek medical aid
immediately.
yWhen disposing of expired reagents, waste liquids, waste samples, and consumables,
comply with local regulations.
WARNING
yUse and store the reagents according to the guide. Failure to do so may negatively impact
performance.
yCheck the expiration date of all reagents before use. Using expired reagents may cause
inaccurate results.
Symbols
Packaging
The following table describes symbols on the packaging or on the label of the
packaging:
Symbol Name Description
This way up Indicates the correct upright position of the crated
until for transport and/or storage
Fragile, handle
with care
Indicates a device that can be broken or damaged
if not handled carefully
Keep dry Indicates a device that needs to be protected from
moisture

Safety Symbols
6
Symbol Name Description
Do not stack
Indicates that stacking of the crated is not allowed
and no item shall be placed on top during transport
or storage
Do not roll
Indicates that the crated shall not be rolled or
turned over. It shall remain in the upright position
at all times
Temperature
limit
Indicates the temperature limits to which the
device can be safely exposed
Humidity
limitation
Indicates the range of humidity to which the device
can be safely exposed
Atmospheric
pressure
limitation
Indicates the range of atmospheric pressure to
which the device can be safely exposed
Device
The following table describes symbols on the device:
Symbol Name Description
General warning sign Signifies a general warning
Warning; biological
hazard Biological hazard warning
Caution; hot surface
Indicates that the marked item can
be hot and should not be touched
without taking proper safety
precautions
Warning; dangerous
voltage
Indicates hazards arising from
dangerous voltages

Safety Symbols
7
Symbol Name Description
Protective earth Indicates the terminal of a protective
earth (ground) electrode
"ON" (power) Indicates the main power supply is on
"OFF" (power) Indicates the main power supply is o
T16AH250VFuse specification Indicates the fuse specification
USB 3.0 port USB connection
Network port Ethernet connection
Warning; laser beam Indicates a laser beam hazard.
Label
The following table describes symbols on the labels of the device or reagent kit:
Symbol Name Description
Research Use
Only
/Indicates a device that is for research use only,
and cannot be used for clinical diagnosis
Model number Indicates the model number or type number
of a product
Manufacturer Indicates the name and address of the device
manufacturer
Date of
manufacture
Indicates the date when the device was
manufactured
Consult
instructions
for use
Indicates the need for the user to consult the
instructions for use

Safety Symbols
8
Symbol Name Description
WEEE symbol
Indicates that waste electrical and electronic
equipment must not be disposed of as
unsorted muni c ipal waste and must
be collected separately. Please contact
an authorized representative of the
manufacturer for information concerning the
decommissioning of your equipment.
User manual
The following table describes symbols that are used in this user manual:
Symbol Description
DANGER
Indicates that the operator should operate the device according to
the instructions in this manual Otherwise, it will result in death or
serious injury
WARNING
Indicates that the operator should operate the device by following
the instructions. Otherwise, it might result in death or serious injury
CAUTION
Indicates that the operator should operate the device by following
the instructions. Otherwise, it might result in minor or moderate
injury
Indicates that the operator should pay special attention to the note
information, and operate the device by following the instructions
Indicates biological risk. The operator should operate the device by
following the instructions

9
02
Device overview
This chapter describes the intended use, working principle, and
structural composition of the device.

Device overview Intended use
10
Intended use
This device is a sequencing instrument that measures optical and electronic
signals of the reporting molecules, which decode the sequence information of
a DNA or RNA fragment. This is accomplished through the use of instrument
specific reagents, flow cells, imaging hardware, and data analysis software. The
sequencing input is intended to be prepared as DNA Nanoball (DNB) libraries,
which can be used for whole genome, whole exosome and de novo sequencing.
WARNING
This device is intended only for scientific research and should not be used
for clinical diagnosis.
Working principle
The device adopts the advanced DNA Nanoball (DNB) and the core technology
of combinatorial probe-anchor synthesis (cPAS). It uses a regular, arrayed flow
cell with special surface sites. Each of the sites contains a single DNB, which are
evenly arrayed across the flow cell, ensuring that the optical signals of nearby
Nanoballs cannot be interrupted by each other. This improves the accuracy of
signal processing.
The following figure demonstrates how to make DNBs:
Ad-inserted
ss DNA circle
Replicate
DNA Nanoball (DNB)
N×
3×
2×
1× DNBs
Figure 1 Making DNBs

Device overview Sequencer overview
11
The following figure demonstrates how to load DNBs:
DNBs
Flow cell
Decorated sites evenly spread on the flow cell
Each site contains a single DNB
Figure 2 Loading DNBs
The DNBs and sequencing reagents are pumped into the sequencing flow
cell through the device’s liquid delivery system. Each DNB combines with the
respective fluorescence group. The laser excites the fluorescence group to emit
light, and the optical signals are acquired by the camera. The optical signals are
converted to digital intensities and processed by the computer to determine the
nucleotide sequence of the DNB.
Sequencer overview
Structural composition
The device consists of the main unit and pre-installed control software (software
version: V1). The main unit includes the main structure, host, optical system,
XYZT-stage, flow cell stage, gas-liquid system, electric control system, reagent
storage system, power supply system and display system.
The following table describes the function of each component:
Component Description
Main structure Provides stable support for the main unit.
Host Controls the device, collects, analyzes, and stores data.
Optical system Images the fluorescence signal on the flow cell.
XYZT-stage Moves the flow cell and focuses automatically.

Device overview Sequencer overview
12
Component Description
Flow cell stage Connects the flow cell to fluidics lines and controls the
temperature of the flow cell.
Gas-liquid system Provides the gas-liquid support that is required for the
biochemical reaction.
Electric control system Controls the electric system.
Reagent storage system Provides the reagent storage environment.
Power supply system Provides the power supply for the device.
Display system Provides the human-computer interaction interface.
Basic components
Front view
1
2 2
3
4
5
6
Figure 3 Front view

Device overview Sequencer overview
13
No. Name Description
1Status indicator
Displays the current status of the device:
yGreen: the device is running.
yBlue: the device is in standby status.
yYellow: a warning appears, but the
device keeps running.
yRed: an error occurred.
2Supporting feet Supports the main unit to ensure stability.
3Latch of the reagent compartment
Press the button on the latch, and pull
the pop-up ring to open the reagent
compartment door.
4Reagent compartment Holds the reagent cartridge and tube at
appropriate temperatures.
5Buzzer Alerts when warnings appear,or errors
occur.
6Touch screen monitor Facilitates on-screen operation and
displays information.