Microtec CUT4060E User manual
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R
OTARY
M
ICROTOME
CUT4060E
Operating Manual
MADE IN GERMANY
microTec Laborgeräte GmbH
Rudolf-Diesel-Str. 47
69190 Walldorf, Germany
Phone: + 49 (0) 6227 8222-0
Fax: + 49 (0) 6227 8222-30
www.micro-tec.de
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Table of Contents
1. General Notes ………………………………………………………………… 4
1.1 Significance of the Operating Instructions ……………………………………4
1.2 Usage According to its Specification ………………………………………… 5
1.3 Improper Use ............................................................................................. 5
1.4 Warranty and Liability …………………………………………………………. 6
1.5 Warranty ………………………………………………………………………… 6
1.6 Brief Discription of Use, Function and Sequence ………………………….. 7
1.7 Edition, Date of Issue of the Operating Manual ……………………………. 8
1.8 Copyright ……………………………………………………………………….. 8
1.9 Address of Manufacturer ……………………………………………………… 8
2. Basic Safety Instructions …………………………………………………… 9
2.1 Heed the Notes and Instructions Given in this Operating Manual ……….. 9
2.2 Obligation on the Part of the Operator ………………………………………. 9
2.3 Obligations on the Part of the Operating Staff ……………………………… 9
2.4 Hazards and Dangers in Handling and Using the Instrument ……………. 10
2.5 Interpretation of Hazard Symbols ……………………………………………. 11
2.6 Informal Instructions Issued by the Manufacturer ………………………….. 12
2.7 Particular Zones of Danger …………………………………………………… 12
2.8 Servicing and Fault Clearance ……………………………………………….. 13
2.9 Structural Modifications and Replacements to the Instrument …………….13
2.10 Cleaning and Associated Care ………………………………………………..14
3. Transportation and Storage …………………………………………………15
3.1 Dimensions and Weights ………………………………………………………15
3.2 Instructions for Transportation …………………………………………………15
3.3 Packaging ………………………………………………………………………..15
3.4 Inspection upon Acceptance by the Recipient ……………………………….15
3.5 Reporting and Documenting Transportation Damage ………………………15
3.6 Storage temperature ……………………………………………………………15
4. Data and Information Concerning the Instrument ………………………16
4.1 Designation ………………………………………………………………………16
4.2 Scope of Supply …………………………………………………………………16
4.3 Packaging ………………………………………………………………………..17
4.4 Ambient Temperatures during Operation …………………………………….18
4.5 Performance …………………………………………………………………….18
5. Installation, Assembly, Commissioning …………………………………..19
5.1 Installation ……………………………………………………………………….19
5.2 Transportation Locking Pieces and Securements …………………………..19
5.2.1 Rearview ………………………………………………………………………...19
5.3 Controls, Indicators and Displays ……………………………………………. 20
5.3.1 Operating panel …………………………………………………………………21
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6. Operation ………………………………………………………………………. 22
6.1 Preparation, Orientation and Alignment of Specimen ………………………22
6.2 Commencing the Cutting Operation …………………………………………. 22
6.3 Instructions for Producing Sliced Microtomic Sections of the Specimen ... 22
6.4 Cutting Action …………………………………………………………………... 23
6.5 Handwheel ……………………………………………………………………… 23
6.6 Coarse Advanced Drive ………………………………………………………..23
6.7 Friction Clutch on the Coarse Advanced Drive Mechanism ………………. 24
6.8 The Handwheel of the Coarse Advanced Drive Mechanism (11) …………24
6.9 Collision Protection Device ……………………………………………………24
6.9.1 Emergency shutdown ………………………………………………………….24
7. Setting Up and Changing the Setup …………………………………….. 25
7.1 Specimen Fixture ………………………………………………………………. 25
7.2 Standard Specimen Clamping Fixtures ……………………………………… 25
7.3 Orientatable Adapter ……………………………………………………………26
7.4 Fixed Adapter …………………………………………………………………… 26
7.5 Cylindrical Specimen Insert and Prism ………………………………………. 27
7.6 Film (Thin Material) Clamping Fixture ……………………………………….. 28
7.7 Universal Cassette Clamping Fixture ………………………………………... 28
7.8 Specimen Orientation Device …………………………………………………. 29
7.9 Exchanging the Specimen Clamping Systems ………………………………30
8. Setting up the Knife …………………………………………………………...31
8.1 Knife Holder …………………………………………………………………….. 31
8.2 Knife Holder Base ……………………………………………………………… 31
8.3 The “R“ Type Knife Holder ……………………………………………………. 32
8.4 The “RS“ Type Knife Holder ……………………………………………………33
8.5 Holder for Throw-away Blades ……………………………………………….. 34
8.6 Clearance Angle Adjustment …………………………………………………. 35
9. Optional Accessories …………………………………………………………35
10. Emergency Service, Customer Service ……………………………………35
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1.
General Notes
1.1 Significance of the Operating Instructions
Dear Customer,
Please read through the operating instructions carefully before using the equipment
and familiarize yourself with its functions and controls. This is imperative to ensure
proper use of the equipment.
Please check the production serial number against the number given on the microTec
identification plate of your unit. It will facilitate faster service and response to queries by
quoting the production serial number.
microTec Production Serial-Number: 60-_____
microTec Laborgeräte GmbH
Rudolf-Diesel-Str. 47
69190 Walldorf, Germany
Phone: + 49 6227 8222-0
Fax: + 49 6227 8222-30
www.micro-tec.de
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1.2 Usage According to its Specification
The CUT 4060E rotary microtomes are manually operated instruments for use in all
work involving paraffin wax and solid cut microtomology within the disciplines of
biology and medicine, and within industry at large.
The specimen can be moved back and forth by means of the coarse wheel mechanism
so to attain rapid advance of the specimen in relation to the knife during cutting.
Owing to the design of its drive, the CUT 4060E rotary microtome enables the user, to
carry out the cutting action manually by means of the handwheel, with locked handle,
and semi -automatically by means of the hand control panel.
The micro-drive mechanism enables feed settings to be made within the range of 0,5
to 60 microns.
The user friendly throw-away blades and universal knife holder with its integrated knife
guard enables trouble-free clamping and setting of the knife blade. The integrated
safety device to prevent any collisions occurring, which is incorporated in the
micrometre mechanism, disables any adjustment to the feed setting being made upon
reaching its maximum setting.
The CUT 4060E rotary microtome is equipped with an electronic cutting counter, which
indicates the total number of cuts made.
To prevent damage occurring to the knife or the specimen, the rotary CUT 4060E
microtome imparts an action of slight retraction of the specimen by approx. 80 microns
during the motion of advancement.
The following kinds of drive may be carried out by means of the hand control panel:
Inching service, single stroke, and continuous stroke. A foot switch is optionally
available.
1.3 Improper Use
The rotary microtome is designed and manufactured to the latest state-of-the-art
technology and to recognized principles of technical and engineering safety.
Nevertheless, dangers can arise during its use to endanger life and limb of the
operator or third persons or damage to the instrument or to other property. The
instrument is only to be used
•for use according to its specification of intended use
•in a proper technical state of repair, free of any defects
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1.4 Warranty and Liability
The company – microTec GmbH – certifies that this instrument has been carefully
tested and inspected. Verification to ensure adherence to the technical specification
data published has been carried out prior to shipping out the instrument from the
factory.
No liability is assumed for the correctness of technical data and other data or drawings.
Reserve technical changes.
The instrument is assigned to Class 4 of the MedGV (legislation governing medical
supplies). Observance of existing legislation and regulation is assured.
1.5 Warranty
Warranty is provided for this microTec product in the event of any deficiencies or
defects in material or workmanship for a period of 12 months. Defective parts will be
repaired or replaced free of charge during this period of time. Any additional claims
made under the warranty of whatsoever nature are excluded. Neither does the
warranty provide cover for any claims arising from services and supplies provided by
third parties.
In principle, our “General Conditions of Sale and Supply“ shall apply. These
conditions are made available to the prospective operator of the instrument, by, at the
latest, the signing of the contract. Warranty and liability claims for personal injury and
material damage are excluded if attributable to one or more of the following causes:
•Improper use of the instrument!
•Incorrect installation and assembly, commissioning, operation and servicing of
the instrument!
•Operating the instrument with the safety devices defective or safety
mechanisms and guards inappropriately fitted!
•Disregarding instructions given in the operating manual concerning
transportation, storage, installation, commissioning, servicing and equipping of
the instrument!
•Unauthorized tampering and structural modification to pneumatic, electrical and
mechanical components!
•Ineffective or incorrect monitoring of machine parts which are subject to wear!
•Improperly effected repairs!
•Catastrophes caused by external influences and force majeure!
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1.6 Brief Description of Use, Function, and Sequence
Use
The CUT 4060E rotary microtomes are manually operated instruments for use in all
work involving paraffin wax and solid cut microtomology within the disciplines of
biology and medicine, and within industry at large.
Function and Sequence
Manually rotating the handwheel on the rotary microtome translates into vertical
movement of the fixture holding the specimen. To produce the cut, resharpenable
knives or throw-away blades are used, which are adapted and attached to the various
knife holders.
A microtomic cut is made during the downward motion of the specimen (cutting action).
By continuing to rotate the handwheel further in a clockwise direction the specimen
starts to rise again in its return motion.
Owing to the different types of operation, cuts may be carried out manually by means
of the handwheel, semi-automatically by means of the hand control panel, and by
means of the foot switch, that will have to be separately ordered.
The selected feed for the thickness of cut is instigated at top dead center.
Rapid advance of the specimen towards the knife is achieved by means of the coarse
feed mechanism.
A universal clamping fixture for holding a variety of specimens is provided.
As a result, simple alignment to the plane of cut is possible using the facility for
orientating the specimen.
The various knife holders enable trouble-free use of a variety of knife types and
thereby provide facility for making adjustment to suit the application in question.
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1.7 Edition, Date of Issue of the Operating Manual
Edition: Version 004.96
Date of issue: 13. February 1996
1.8 Copyright
Copyright : microTec Laborgeräte GmbH
Copyright to this operating manual remains with microTec GmbH. This operating
manual is only intended for use by the operator and his staff.
It contains specifications and instructions, which may not be
-
copied, distributed or otherwise divulged to others in their entirety or in part.
Infringements can lead to legal proceedings being brought against the perpetrators.
1.9 Address of the Manufacturer
microTec Laborgeräte GmbH
Rudolf-Diesel-Straße 47
69190 Walldorf, Germany
Phone: + 49 (0) 6227 82 22-0
Fax: + 49 (0) 6227 82 22-30
www.micro-tec.de
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2. Basic Safety Instructions
2.1 Heed the Notes and Instructions Given in this Operating
Manual!
A prime prerequisite for safe handling and malfunction-free operation of this instrument
is the awareness and recognition of basic safety instructions and regulations.
This operating manual contains the most important instructions for operating the
instrument in a safe manner.
This operating manual, especially the instructions on safety, are to be heeded by all
personnel who operate the instrument.
In addition, compliance with the regulations and specifications on accident prevention
applicable for the site of installation and use is to be maintained.
2.2 Obligation on the Part of the Operator
The operator is obliged to only allow personnel to operate the instrument, who
•are familiar with basic regulations on safety at work and accident prevention,
and are trained in the use and operation of the instrument.
•have read the chapter on safety and the warning notes and instructions given in
this operating manual.
•have been taught to have due regard to aspect of safety during the execution of
their work.
2.3 Obligations on the Part of the Operating Staff
All personnel who are delegated with the task of operating this instrument are obliged
before starting work
•to heed the basic regulations on safety at work and accident prevention.
•to read and to comprehend the chapter on safety and the warning notes and
instructions given in this operating manual.
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2.4 Hazards and Dangers in Handling and Using the Instrument
Important!
The following precautionary measures must be undertaken during any
operation carried out using the instrument. Non-compliance with these
precautionary measures will infringe recognized principles and rules of
technology and the intended and proper use of the instrument. The
manufacturer – microTec GmbH – accepts no
liability in the event of such infringement.
Risk of Injury
The finger guard must be used when setting the knife and specimen,
in order to reduce the risk of injury on the knife/blade.
Always insert the specimen first.
Then insert the knife into the knife holder.
Before exchanging the clamping device, always remove the knife or
blade. Knives, which are not being used, must
always be stored in the knife box. Never attempt to
catch a falling knife. Take care when cutting or
removing a sliced section.
Risk of Infection
Appropriate measures regarding protection and disinfection are to be
taken when working with infected material.
Danger due to Radio Activity
The current applicable regulations for the protection against radiation
must be observed at all times when working with radio active
materials or specimens.
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2.5 Interpretation of Hazard Symbols
Interpretation
Symbol
•Danger
•Warning
•Caution
•Note
•Important !
•Information
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2.6 Informal Instructions Issued by the Manufacturer
Preparation of the Specimen
Due attention should be paid in the preparation of specimens with regard to making the
correct choice of the medium used for embedding and encapsulation of the specimen
and its curing and infiltration time.
Temperature of the Specimen
Microtomic sectioning is generally carried out at room temperature (except in the case
of frozen specimens). Increases in temperature will soften the paraffin wax. The
heating up of paraffin wax specimens due to direct exposure to sunlight or their
proximity to any other source of heat is to be avoided.
Choosing the Knife
Depending upon the ground facet in question, a clearance setting at an angle of 18 to
20 degrees is to be selected. In the case of re-sharpened knives, use the knife height
adjustment facility to set the height of the knife.
Levers and Clamping Screws
Securely tighten all levers and clamping screws on the knife holder and the specimen
orientation device. > Overtightening could result in damage of knife holder!!
Cutting Speed
Rule: “The harder the material, the slower the cutting speed“.
Starting the Cut
Take care when approaching the knife or the specimen.
2.7 Particular Zones of Danger
Important!
Knives are sharp!
Always set the knife guard in its proper position after completing the operation – even
if it’s for a brief moment of time – and make sure it is properly seated.
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2.8 Servicing and Fault Clearance
Servicing
To secure the quality of the microtomic section and operational reliability of the rotary
microtome, a routine service should be carried out once a year. Servicing should only
be carried out by a trained service engineer.
Service Contract
microTec offers a service contract which guarantees that your instrument will remain in
a perfect operational condition.
Fault Clearance
Malfunctions, which can adversely affect safety, are to be rectified immediately.
2.9 Structural Modifications and Replacements to the Instrument
•No modifications, conversions or enhancements may be made to the instrument
without the approval being given by the manufacturer.
•All conversions require written confirmation issued by microTec.
•Replace components which are not in proper working order immediately.
•Only use original replacement and consumable parts and items.
•It cannot be guaranteed that parts sourced from other suppliers are
designed and manufactured to meet safety and reliability conditions.
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2.10. Cleaning and Associated Care
Cleaning
Depending upon the level of dirt and contamination and upon the
frequency of use, regular cleaning and care of the rotary microtome is
recommended. The instrument is best cleaned using a mild domestic
cleaning agent. Do not use harsh solvents or cleaning compounds,
because paintwork and plastic components can be damaged by their
use.
Always remove the knife and stow in the knife box
prior to cleaning the instrument.
Important!
Never place a knife on a table or surface with the cutting edge
facing upwards. Thoroughly clean the bearing surfaces and contact
faces and the guide of the knife holder.
Associated Care
When the instrument is not in use, turn back the cylinder by means of
the coarse drive mechanism and lock the handwheel. Cover the instrument
with the cover supplied.
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3. Transportation and Storage
3.1 Dimensions and Weights
Dimensions
Width: .…….…… ……………. 460 mm
Depth: ….……...…………….… 460 mm
Height: ..…………………..…….. 280 mm
Weight
Weight (without accessories) ……..48 kg
3.2 Instructions for Transportation
Lock the handwheel.
Remove all knives, knife holders, specimen
fixtures and specimens from the rotary
microtome.
3.3 Packaging
The rotary microtome has been supplied in a
special packaging designed for transportation.
Do not commission (start-up) the instrument
until al least 24 hours have elapsed following transportation.
Please ensure the specified procedures are strictly!
Important!
The unit is heavy – do not lift or carry the instrument by means of
the handwheel.
3.4 Inspection upon Acceptance by the Recipient
Firstly, check that all items ordered are supplied in the transportation crate. Inspect the
transportation crate and the rotary microtome for any damage incurred during transit.
3.5 Reporting and Documenting Transportation Damage
In the event of damage caused in transit, the manufacturer and the forwarding agent
are to be advised immediately in writing.
3.6 Storage temperature
Storage temperature range +5°C to + 55°C.
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4. Data and Information Concerning the
Instrument
4.1 Designation
Rotary Microtome, model CUT 4060E
4.2 Scope of Supply, i.e.
According to order confirmation.
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4.3 Packaging
1
st
Step
Remove all side and top sitting
screws sides and take the complete
cover of the transportation crate.
2
nd
Step
Remove all hexagon headed
screws around and the packaging
frame.
3
nd
Step
With both hands lift the instrument
out from the transportation crate.
Important!
The unit is heavy – do not lift or
carry the instrument by means of
the hand wheel.
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4.4 Ambient Temperatures during Operation
+ 10°C to +35°C
Contamination level 2
Overvoltage categorie II (CUT 4055E & CUT 4060E)
4.5 Performance
Range of section thickness: 0.5 - 60 µm
Increments: 0.5 µm from 0.5 µm to 2 µm
1 µm from 2 µm to 10 µm
2 µm from 10 µm to 20 µm
5 µm from 20 µm to 60 µm
Horizontal object advance Max. 40 mm
Vertical stroke Max. 70 mm
Specimen size: standard: 55 x 50 mm
Specimen orientation: X- and Y-Axes 8°, Z-Axis till 360°
Cutter drive: motorized manual switchover
Power consumption: P max. 150 Watt
Max. speed 150 rpm
*according to contractual agreement
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5. Installation, Assembly, Commissioning
5.1 Installation
Protect the instrument from:
•sources of heat such as fan heaters or hot air outlets ;
•extremely low temperatures;
•direct sunlight;
•excessive dust;
•moisture or rain;
•mechanical vibration or impacts;
•equipment which contain magnets or generate magnetic fields.
5.2 Transportation Locking Pieces and Securements
Remove all locking pieces and adhesive tape used for securement during transportation.
•Place the instrument on a rigid, vibration-free table or bench.
•Align the instrument on the table. Lift up the instrument a little at the front.
•Put the mains plug into the socket provided for this purpose.
•See to correct voltage of your unit and of the mains.
Important! Allways use a 3-conductor, grounded wall outlet.
•Connect the hand control panel to the socket provided for this purpose at the
rotary microtome (see figure).
•Connect the optionally available foot switch.
•Connect the optionally available flush-mounted illumination.
5.2.1 Rearview of the rotary microtome
Picture 3
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5.3 Controls, Indicators, and Displays
Item Description
1 Handwheel for cutting operation
2 Handwheel handle for handwheel 1
3 Handwheel locking device
4 Handwheel control panel
5 Rotary knob for setting the thickness of slice
6 Inspection window for setting and inspecting slice
thickness
7 Locking lever for knife holder
8 Lever for trimming adjustment
9 LCD-display (cutting counter)
10 Reset for LCD-display
11 Handwheel for coarse drive
12 Clamping mechanism for knife holder
13 Guide bars for knife holder
14 Foot switch
15 Step Operation
16 Single stroke
Continuous stroke
17 High Speed Key Foot switch
20 Emergency shutdown
LCD-display
6
15
7
5
2
3
1
8
9
11
4
12
13
20
16 17
10
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Table of contents
