Genevac EZ-2 Elite User manual

Genevac EZ-2 Elite
Personal Evaporator
User Manual
Original instructions
Part number 04-6921 Issue 3-3 August 2014

Contents
Overview ......................................................2
Safety............................................................4
Warnings and cautions ...................................4
Risk assessment ............................................4
Precautions.....................................................4
Combustible solvents .....................................5
Electrical earthing ...........................................5
Emergency shutdown .....................................5
Strong acids....................................................6
Low auto-ignition point solvents .....................6
Basics...........................................................7
Power up.........................................................7
Standby mode ................................................7
Controls ..........................................................8
LCD Screen ....................................................9
Opening the Lid ............................................10
Getting started ........................................... 11
Selecting a method.......................................12
Sample temperature .....................................12
Loading Samples..........................................13
Use of correct accessories ...........................13
Sample holders.............................................13
Mass limit......................................................14
Loading the rotor ..........................................14
Starting the evaporator .................................15
Stopping........................................................15
Evaporation in progress................................15
Pausing.........................................................15
Pump warm up .............................................16
Pump purge ..................................................16
Condenser..................................................17
Waste solvent ...............................................17
Automatic defrost and drain..........................17
Manual defrost and drain..............................18
Condenser back-light....................................18
Waste solvent container ...............................18
Method Guide.............................................19
Solvent groups..............................................19
Acceptable solvents......................................20
Choosing the right method ...........................21
Solvent mixtures ...........................................21
Low solvent volumes ....................................21
Custom methods ..........................................21
Advanced Features....................................22
File transfer...................................................22
Adjustable method parameters ....................23
Time to final stage ........................................24
Final stage time ............................................25
Reduce odour ...............................................25
Set max temp ...............................................26
System information.......................................26
Method ID .....................................................26
View method .................................................26
Dri-Pure®.......................................................26
Wait to cool ...................................................27
Wait to heat...................................................27
Inert Gas Purge ..........................................28
Inert gases ................................................... 28
Set Password............................................... 28
Inert gas purge enable................................. 28
Inert gas blanket .......................................... 29
Disconnection .............................................. 29
Maintenance ...............................................30
Recommended practice............................... 30
System test .................................................. 31
Periodic maintenance .................................. 31
General cleaning.......................................... 32
Sample holders............................................ 32
IR sensor...................................................... 32
Inert gas purge systems .............................. 33
Planned maintenance and servicing............ 33
IR lamp assembly ........................................ 34
Lid seal......................................................... 36
Software upgrade ........................................ 37
Unpacking and Setting Up.........................38
Packaging .................................................... 38
Lifting ........................................................... 38
Installation site ............................................. 38
Service connections..................................... 39
Setting up..................................................... 42
Installing systems with inert gas purge ........ 44
Final checks ................................................. 44
Troubleshooting.........................................45
Audible and visual alerts.............................. 45
Faults and errors.......................................... 45
Rectifying faults or errors............................. 46
Clearing faults and errors ............................ 48
IR lamp failure.............................................. 48
Opening lid without power ........................... 48
Fuses ........................................................... 49
Other troubleshooting .................................. 50
Options .......................................................52
Standard equipment .................................... 52
Infinity trolley ................................................ 52
HCl resistance ............................................. 52
Inert gas purge............................................. 52
Sample Holders ........................................... 53
Technical Data............................................54
Specifications............................................... 54
EC Declaration of Conformity ...................... 55
Warranty statement...................................... 56
Consumable items ....................................... 56
Returning equipment to Genevac ................ 56
Patents......................................................... 57
Amendment control...................................... 58
Useful information........................................ 58

Genevac EZ-2 Elite Personal Evaporator
User Manual
204-6921 Issue 3-3 August 2014
Overview
1
5
6
2
3
4
11
10
9
8
7

User Manual
Genevac EZ-2 Elite Personal Evaporator
04-6921 Issue 3-3 August 2014 3
Item
Feature
Function
1
USB port
(On side panel.) Data log files may be transferred
to, method files may be transferred to or from a
USB data key.
2
LCD screen
Menus allow quick access to everyday functions
and intuitive guidance to advanced features.
3
SET MAX TEMP °C
Easily set the maximum sample temperature.
4
SELECT control
Twist to highlight menu options on the LCD screen,
press to select.
5
Start, stop, pause buttons
Control the evaporation process using the selected
method.
6
Vacuum pump
Low maintenance, super high vacuum pump
provides wide solvent compatibility from low to high
BP.
7
Rotor
Compatible with a wide range of Genevac sample
holders which can accommodate most common
sample formats: round bottom flasks (up to 500 ml
in volume), tubes (up to 160 mm in length), custom
reaction blocks and shallow or deepwell
microplates. EZ-2 is also compatible with the
revolutionary Genevac Sample Genie™ system.
8
Lid
Unlocks only when safet to open. Press the LID
button and manually lift the lid to when a beep
sounds.
9
IR sensor access panel
Easy access to the IR lamp when replacement is
required.
10
Auto drain condenser
Highly efficient cold trap captures all solvent as
liquid (even water) and drains automatically.
11
Quick reference guide
At-a-glance guidance to basic operation.

Genevac EZ-2 Elite Personal Evaporator
User Manual
404-6921 Issue 3-3 August 2014
Safety
Warnings and cautions
This symbol is used within this manual to highlight Warnings and Cautions.
Warning: highlights a risk of personal injury or material damage.
Caution: highlights a risk of material damage.
Risk assessment
Caution: Carry out a risk assessment for each solvent before use. Consider the
possible consequences of applying vacuum to solvent mixtures, especially
organic solvents. For example, H2O2gives off O2under vacuum, creating an
oxygen rich atmosphere.
Precautions
Observe the following safety precautions when using the evaporation system:
Only operate the system in a ventilated space.
Make sure exhaust / ventilation hoses are securely fitted and take solvent vapours away
from the equipment and from the operator.
Only use sample holders and / or swings that are approved for use by Genevac.
Do not leave the system unevenly loaded, someone may start it.
Do not start or restart a system without checking it is evenly loaded.
Do not place objects on top of the evaporator.
Check the condenser has been drained before starting the evaporator.
Take care when removing the condenser jar. It is heavy when full of solvent.
Inspect the condenser jar frequently. Do not use it if it is cracked or damaged in any way.
Do not allow the condenser jar to fill above the MAX line.
Do not leave samples in the evaporator before or after evaporation.
Refer to the Genevac published list of acceptable solvents for solvent compatibility
information.
Clean up any solvent spillage immediately.
Dispose of waste solvents in accordance with local environmental regulations.
Caution: Incorrect loading may result in damage to samples and to the system.
Only allow users who are familiar with all the issues outlined in this User Manual to operate
the equipment. If personnel lack the training or experience to comprehend the hazards that
can arise when operating the system, do not allow them to use it; personnel without such
training require thorough instruction. The information contained within this User Manual may
form the basis of such training.

User Manual
Genevac EZ-2 Elite Personal Evaporator
04-6921 Issue 3-3 August 2014 5
Combustible solvents
Warning: Risk of vapour ignition. Only operate the system in a well-ventilated
environment and consider safety when evaporating any combustible solvents.
Genevac's position regarding the evaporation of such solvents, particularly with
respect to the European ATEX directive, is available on our website, at
www.genevac.com, or from your local Genevac representative.
Electrical earthing
Warning: Risk of electric shock. This equipment must be earthed. The
evaporation system is a safety class I product according to IEC classification. It
must never be used with any interruption to the safety earth conductor. For
systems with multiple power leads, each lead requires a separate earth
connection and must be plugged into a separate power socket which is
connected directly into the laboratory electrical supply; do not use extension
leads.
This equipment is an installation class II product and is intended to operate from a normal
single-phase supply.
Emergency shutdown
All Genevac evaporators are designed to be safe with respect to samples when they are
switched off in an emergency. The system will automatically vent and the rotor will spin
down slowly when power is removed. To shutdown in an emergency, switch off the power
sockets at the wall and unplug (or simply unplug if there are no switches). Users should
familiarise themselves with the location of the connections to the mains supply and make
sure the power sockets and plugs are always accessible.

Genevac EZ-2 Elite Personal Evaporator
User Manual
604-6921 Issue 3-3 August 2014
Strong acids
Caution: Do not attempt to evaporate, HCl,
thionyl chloride, or other acid chlorides, in
non-HCl compatible evaporators.
The solvent compatibility of the EZ-2 Elite evaporator
is indicated on the lid. If the lid is marked
NOT HCl COMPATIBLE do not attempt to evaporate
this class of solvent. If the lid is marked
HCl COMPATIBLE the system is compatible with
HCl.
Note: HCl COMPATIBLE implies only resistance to
HCl vapours. Any liquid spillage should be cleaned
up immediately and in compliance with your local
solvent spillage procedures.
Low auto-ignition point solvents
Warning: Risk of vapour ignition. Do not attempt to evaporate diethyl ether,
pentane or other low auto-ignition solvents unless the evaporator is fitted with an
inert gas purge system.

User Manual
Genevac EZ-2 Elite Personal Evaporator
04-6921 Issue 3-3 August 2014 7
Basics
Power up
Before switching on, check that power is connected to the EZ-2 Elite via two power cables,
as shown in: Service connections.
Switch on the EZ-2 Elite using the power switch on the front.
The display shows: UPDATE MANAGER –LOOK FOR USB DATAKEY while the EZ-2
performs a short software self-test. The software version is then shown briefly before the
EZ-2 Elite powers up fully.
Standby mode
While the system is idle, press the STOP button to enter standby mode.
In standby mode:
The backlight LCD screen shows the software version.
The LCD screen back-light is switched off.
The condenser is powered down.
The lid cannot be opened.
Methods cannot be viewed.
Settings cannot be adjusted.
To restart the system, press any button.
The evaporator automatically switches to standby mode if inactive for more than one hour.
This feature allows the system to be left unattended; it will evaporate the samples safely and
turn itself off when done.
Power switch

Genevac EZ-2 Elite Personal Evaporator
User Manual
804-6921 Issue 3-3 August 2014
Controls
1. Display: LCD screen with back-light, provides icon, text and numeric information for
programming methods and monitoring evaporation progress
2. SET MAX TEMP °C control: Turn to adjust sample temperature setting.
3. SELECT control: Turn and press to browse and select method options.
4. Start / Stop buttons: Press the appropriate button once to start or stop an evaporation.
5. Pause button: Press once to pause and again to restart an evaporation.
6. Lid LED: Green LED lights up when it is safe to open the lid.
7. LID unlock button: Press the button once to unlock the lid.
8. Dri-Pure LED: Yellow LED lights up when selected method includes a Dri-Pure stage.
4
3
2
1
5
8
7
6

User Manual
Genevac EZ-2 Elite Personal Evaporator
04-6921 Issue 3-3 August 2014 9
LCD Screen
The LCD screen provides information relating to method selection, method progress or
evaporator status.
Lower screen section
Displayed text relates to
evaporator status,
methods or errors.
1. Temperature: The sample holder temperature during evaporation.
2. Pressure: The chamber pressure, from atmospheric to ultimate system vacuum.
3. Time: The time remaining (when known) or the time elapsed. An arrow next to the
displayed time indicates whether the timer is counting down (time remaining) or counting
up (time elapsed). Note: Time remaining is unknown throughout most of the
evaporation when the method setting: Run to AutoStop is selected.
4. Method description: Available methods and method parameters, interacts with the
SELECT control to allow the user to browse and select settings.
5. Status: The current status of the evaporator. If a fault or error is encountered, a
reference number and a brief description of the problem are shown.
6. System busy indicator: Animates to show when the system is waiting for a process to
complete.
7. Progress indicator: Shows sample drying progress from 80% to 20% completion.
Segments within the test tube icon extinguish sequentially, use this for approximate
guidance only. A sample pellet icon, in the bottom of the test tube, flashes to indicate
when the evaporation is complete.
3
4
2
1
5
6
7

Genevac EZ-2 Elite Personal Evaporator
User Manual
10 04-6921 Issue 3-3 August 2014
Opening the Lid
The lid cannot be opened when the rotor is moving or when the chamber pressure is below
atmospheric pressure. When the evaporator stops, a countdown timer appears on the
display. If the LID button is pressed while the evaporator is operating, or during the
countdown, a double beep sounds and the lid remains locked. The countdown also appears
when the evaporator is first powered up.
When the countdown reaches 0, a short beep sounds and the lid LED lights up to indicate
that the lid can be opened.
The lid also remains locked when the system is switched off or when the mains power is
disconnected.
Lid LED
LID button

User Manual
Genevac EZ-2 Elite Personal Evaporator
04-6921 Issue 3-3 August 2014 11
Getting started
The following guide is a quick reference reminder for getting started. For more detailed
operating instructions and descriptions of features, refer to the relevant sections within this
user manual.
Start-up procedure
1. Switch on the power.
2. Press the START button and wait for the
safety countdown to complete.
3. Make sure the condenser has been
drained.
4. When the lid LED lights up, press the
LID button.
5. Open the lid.
6. Load the balanced sample holders onto
the rotor.
7. Close the lid.
Using the SET MAX TEMP °C control, set
the desired maximum temperature.
8. Using the SELECT control, select the
required method.
9. Press the START button.
7
9
8
4
1
3
2

Genevac EZ-2 Elite Personal Evaporator
User Manual
12 04-6921 Issue 3-3 August 2014
Selecting a method
In idle mode, turn the SELECT control. The available
methods appear in the lower section of the LCD
screen.
When the required method is shown, press the control
to select it.
Sample temperature
Turn the SET MAX TEMP °C control to set the desired
sample control temperature. The control temperature
appears in the lower section of the LCD screen and
increments up or down as the menu control is turned.
Stop turning when the desired control temperature is
displayed, the new setting is accepted after a short
time and the display reverts to showing the method
status.
Once evaporation commences, the EZ-2 controls the
temperature of the sample holders; the sample control
temperature may be adjusted at any time during
operation or when the EZ-2 is in idle mode.
Note: The evaporator controls the sample holder temperature. Due to evaporative cooling
the actual sample temperature during evaporation may be considerably lower than the
sample holder temperature. The sample control temperature should be considered the
maximum temperature (or cut-off point) that the EZ-2 will allow the samples to reach.
Some methods specify a sample control temperature and will not allow adjustment by the
user. For these methods, sample temperature control is locked out and the display shows a
padlock icon if the user attempts to adjust the setting.

User Manual
Genevac EZ-2 Elite Personal Evaporator
04-6921 Issue 3-3 August 2014 13
Loading Samples
Use of correct accessories
Samples in the evaporator are subjected to
accelerations of up to 500 g (a force equal to
500 times gravity) during evaporation. It is
therefore important for the operator to
understand the safety requirements and to
gain familiarity with the safe loading
procedure outlined below.
Sample holders
A range of sample holders is available to
suite a wide variety of plates, vials, tubes,
beakers, flasks, etc, as well as the
revolutionary Genevac SampleGenie™
system. Sample holders are
interchangeable, lifting straight out of the
rotor to allow samples to be loaded quickly
and easily. Some sample holders are a one-
piece integral holder and swing, others
consist of a sample holder block, which fits
into a separate swing.
Integral swing / tube holder
Tube holder and side bridge swing
To see the range of sample holders
available, contact your local Genevac
representative and request a Genevac
Accessories Brochure.
Take care to make sure that the correct
sample holders are used, follow this
guidance at all times:
Only use swings and sample holders that
are approved for the system (it is
particularly important to check this if
more than one Genevac system is in
use)
Only use the tubes or vials that are
approved for the sample holder
(incompatible tubes may appear to fit a
holder they could be too long when
loaded onto the rotor)
Use matched pairs of sample holders on
opposing positions of the rotor
Do not use sample holders intended for
side-bridge swings in open swings (they
may appear to fit but could be too tall
when loaded onto the rotor).
If using heat transfer plates, insert each
plate into the swing with the flat metal
base plate facing downwards so it
contacts the swing (heat transfer plates
are for use with microtitre plates only).
The Genevac Accessories Brochure
indicates the maximum tube length that each
holder can take and includes notes such as:
For use in side-bridge swing only.
Note: Some Bohdan Miniblock system
sample holders exceed the mass limitations
of Genevac systems. If you plan to use
Bohdan Miniblocks in a Genevac system,
please contact Genevac for a list of the
relevant weights.
Caution: With the exception of
microtitre plates, do not load any
non-Genevac holders into a
system without gaining approval
from Genevac.

Genevac EZ-2 Elite Personal Evaporator
User Manual
14 04-6921 Issue 3-3 August 2014
Mass limit
The mass limit for Genevac EZ-2 evaporators is 1.5 kg per swing. This is the total mass that
can be loaded onto each position of a swung rotor, and includes:
The swing
The sample holder
The tubes or vials
The sample solutions.
Do not exceed the mass limit under any circumstances. In most cases, with normal solvent
volumes, loaded Genevac sample holders will fall within this limit. If in any doubt, load up a
swing and weigh it.
Loading the rotor
Check the condition of sample holders and (where
applicable) swings before loading them onto the rotor.
Refer to Maintenance –Sample holders.
When preparing sample holders to be loaded onto the
rotor, do not exceed the maximum load capacity of 1.5
kg per swing. This is important as samples in the
evaporation chamber can be subject to a centrifugal
force approximately 500 times greater than gravity.
Balance the sample holders to within 10 g.
Distribute tubes in balanced configurations in the
sample holders.
Load the sample holders carefully, making sure they
are located correctly on the rotor pins.
Place both sample holder swings onto the rotor.
Rotate the rotor by hand. Check that all tubes, holders,
plates, etc are correctly located and swings are able to
swing freely. Only use the sample holders that were
supplied with the system or are specified for use with
the system; never use non-Genevac approved sample
holders.
Note: Genevac Ltd cannot accept responsibility for any
loss of samples or damage to the EZ-2 Elite incurred as
a result of improperly or excessively loaded rotors.
Caution: Failure to load a system correctly can lead to unrecoverable sample loss
or damage to the system. As with all centrifuges, Genevac centrifugal evaporators
must be loaded correctly to remove the risk of damage.

User Manual
Genevac EZ-2 Elite Personal Evaporator
04-6921 Issue 3-3 August 2014 15
Starting the evaporator
Stopping
Press the START button to begin
evaporating using the selected method.
Note: Once the evaporator starts, the
method may not be changed.
Evaporation in progress
On the LCD screen, a test tube graphic
animates to show that evaporation is in
progress and the time elapsed or (when
known) time remaining is shown.
The Dri-Pure LED indicator lights up during
Dri-Pure enabled methods. During the Dri-
Pure part of a method, the display shows the
message: Ramping Vacuum.
The message: Defrosting may be displayed
periodically during certain methods, this
indicates the automatic defrost operation of
the SpeedTrap.
A sample pellet icon, in the bottom of the test
tube, flashes to indicate when the
evaporation is complete.
Automatic stop
The evaporator stops automatically when all
stages of the selected method are complete.
Automatic stop can be set to trigger when the
Heatflow detection system senses a slow-
down in the evaporation rate, or can be set to
occur after a preset time. For heatflow to
function adequately, 50% of the holder must
be filled with samples, each tube must be at
least 50% full and the sample temperature
selector must not be set to LAMP OFF.
The Heatflow method is inappropriate in
situations where little or no heat is required
from the IR lamp (such as when small
volumes of volatiles are evaporated at low
temperature). In this instance, the system
runs for a pre-programmed time instead.
The time required is mapped by the software
and depends on the run selected and the
control temperature. If user experience
shows that the default time is longer than
necessary, a shorter time can be set.
Manual stop
To stop the evaporator manually, press the
STOP button. The evaporator vents to
atmospheric pressure and the rotor slows
down and stops. Defrost starts
automatically.
Pausing
Press the PAUSE button to stop the EZ-2
Elite temporarily in order to examine the
samples. To resume evaporation, close the
lid and press the START button. If the STOP
button is pressed, the EZ-2 Elite will reset
and the evaporation cannot be resumed.
Dri-Pure is not applied when resuming from
paused, inert gas purge enabled evaporators
will re-purge when the method resumes.
A defrost may be initiated manually while the
EZ-2 Elite is paused.

Genevac EZ-2 Elite Personal Evaporator
User Manual
16 04-6921 Issue 3-3 August 2014
Pump warm up
Pump purge
The following information only applies to
methods that are specifically set up for HCl
or other acids, and therefore normally only
applies to HCl compatible systems.
In order to protect the vacuum pump from
potentially harmful effects of acid solvent
condensation, the system warms the pump
to a safe operating temperature before
allowing an evaporation to start. Pump
warming begins when the evaporator is
switched on and takes 30 minutes. If an HCl
or acid specific method is selected and the
START button is pressed during the warm-
up period, the EZ-2 Elite spins the rotor to
perform a balance test, then stops to wait for
the pump to warm-up before beginning the
evaporation, the warm-up time remaining
time is shown on the LCD screen.
The system also warms the pump when
restarted from standby; the warm up time is
reduced proportionately if the system has
only been in standby mode for a short time.
At the end of evaporation the vacuum pump
continues to operate for a period to purge
any potentially harmful solvents from the
pump.
Caution: Do not switch off the
EZ-2 Elite during the pump
purge period; this may damage
to the vacuum pump.
When the purge cycle is complete, the EZ-2
Elite automatically switches to standby
mode.

User Manual
Genevac EZ-2 Elite Personal Evaporator
04-6921 Issue 3-3 August 2014 17
Condenser
The condenser powers up when the
evaporator is switched on and is deactivated
if it enters standby mode. When powered,
the condenser jar is illuminated by blue
LEDs. The EZ-2 Elite does not allow
evaporation to commence until the
condenser has cooled down to operating
temperature.
A section around the base of the glass jar
illuminates green to indicate when the
condenser is draining.
Caution: Do not attempt to
remove the condenser jar
assembly. The auto-drain
condenser of the EZ-2 Elite
operates differently from other
EZ-2 systems. If removal of the
condenser jar is necessary,
contact Genevac Service.
Waste solvent
Waste solvents collect as liquid in the
Condenser jar. Under certain conditions, ice
may form in the jar. If this happens, select
defrost. Waste solvent boiling and reflux in
the condenser jar, during evaporation, is
normal.
Automatic defrost and drain
Condenser defrosting and draining is fully
automatic and occurs at the end of every
evaporation, for some methods it also occurs
periodically during evaporation. Once an
automatic defrost and drain cycle
commences it should be allowed to
complete. If the START button is pressed to
initiate a new evaporation during and
automatic defrost and drain cycle, this takes
precedence and the system starts
immediately using the selected method.
The condenser drains automatically before
starting evaporation. The opaque ring
around the base of the condenser jar
illuminates green to show when the
condenser drain valve is open. Whilst
defrosting, the display shows the defrost time
remaining.
Defrosting of the condenser coil is
independent from the condenser jar defrost,
and occurs periodically during evaporation.

Genevac EZ-2 Elite Personal Evaporator
User Manual
18 04-6921 Issue 3-3 August 2014
Manual defrost and drain
In addition to automated defrosting, an
extended defrost cycle can be initiated
manually while the evaporator is paused or
in idle mode.
To activate manual defrost, turn the
SELECT control. When the display shows:
Manual Defrost, press the SELECT control.
The condenser jar illumination flashes to
show the defrost cycle is active. The defrost
lasts for 30 minutes.
To cancel defrost, press the SELECT control
or select a method and press the START
button. The extended defrost cycle cannot
be activated when the EZ-2 is evaporating,
in standby mode, or reporting an error
message.
Condenser back-light
The condenser back light provides
illumination so that the solvent level in the
condenser jar can be seen, in addition the
blue condenser backlight flashes to alert the
user in various modes of operation. For
more information, refer to: Troubleshooting -
Visual alerts.
Waste solvent container
Check the condition of the waste solvent
container, waste solvent drain hose and
exhaust hoses regularly. Drainage of waste
solvent into the waste solvent container by
gravity must be unimpeded by kinks, sharp
bends, uphill sections or other constrictions.
The waste solvent container must be below
the level of the drain outlet and the ends of
the hoses must not be below the maximum fill
level for the waste solvent.
Always empty the waste solvent container
and reconnect it to the system before starting
the evaporator.
Note: The evaporator is supplied with a
polypropylene waste solvent container. It is
advisable to replace this with a glass
container (2.5 litre with GL45 cap) before
evaporating solvents that may attack
polypropylene.
Caution: Waste solvent may
drain periodically whilst the
evaporator is operating. Do not
start the evaporator with the waste
solvent container disconnected,
and keep the waste solvent
container connected while the
evaporator is operating.