MRC PCR-300 User manual

PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION
3, Hagavish st. Israel 58817 Tel: [phone], Fax: [phone] [email protected]
MRC.VER.01-9.11
Operation Manual for
Thermal Cycler 0.2ml x 64
PCR-300

PERFACE
Thankyouforpurchasing PCR-300ThermalCycler.Thismanualdescribesfunctionand
operationprocedureoftheinstrument.Tousetheinstrumentcorrectly,readthismanual
carefullybeforeoperatingtheinstrument.Pleasekeepthismanualforreferencein
caseoffuturetrouble.
UNPACKINGCHECK
Onceitisopened,thepackageshouldbecheckedaccordingtothepackinglist.If
thebuyerfindsanyitemstobemissingordamaged,donothesitatetocontactthe
distributor.

Important
Important
Important
ImportantNote
No te
Note
Note
★
★
★
★This
This
This
Thisinstrument
in strument
instrument
instrumentonly
on ly
only
onlyuse
us e
use
usein
in
i n
inscientific
sc ientific
scientific
scientificresearch,
re search,
research,
research,strictly
st rictly
strictly
strictlyprohibit
pr ohibit
prohibit
prohibitusing
us ing
using
usingin
in
i n
inclinic
cl inic
clinic
clinic
1.
1.
1.
1.Important
Im portant
Important
ImportantInformation
In formation
Information
Informationof
of
o f
ofSafe
Sa fe
Safe
SafeOperation
Op eration
Operation
Operation
Safeoperationoftheinstrumentwithacompleteunderstandingofhowtheinstrument
works.Pleasereviewthewholeoperationmanualbeforeattemptingtooperatethe
instrument.
Donotallowanyonewhohasnotreviewedthemanualtooperatethe
instrument.Ifoperationisnotaccordingtotheinstructionofthismanual,the
heatproducedbyinstrumentcancauseseriousburns,andmaycauseelectric
shock.Pleasepayattentiontothefollowingsafetipsandinstructions,andallof
thesafetymeasuresshouldbetaken.
2.
2.
2.
2.Safety
Sa fety
Safety
Safety
Duringtheoperation,maintenance,orrepairofthethermalcycler,thefollowingsafety
measuresshouldbetaken.Otherwise,theguardprovidedbytheinstrumentislikelytobe
damaged,theratedsafetyleveltobereduced,andtheratedoperationconditionstobe
affected.
Thethermalcycler,complyingwiththeNationalStandardGB9706.1,isa
generalinstrumentofTypeB,ClassⅠ.Itisintendedforindooruse.
Beforeoperatingtheinstrument,readtheinformationinthismanual,ormay
causepersonalinjuryaccident.Ensurethatanyoneoperatetheinstrumenthas
receivedinstructionsinbothgeneralsafetypracticesforlaboratoriesand
specificsafetypracticesfortheinstrument.
Theoperatorisnotallowedtoopentheinstrument,otherwisewillvoidthe
warranty,maycauseelectricshock.Ifthedeviceneedsrepair,isunder
obligationtorepair.
A.C.power’sgroundingshouldbereliableforfearofanelectricshock.The
3-pinplugwiththisinstrument’spowerlineisasafetydevicethatshouldbe
matchedwithagroundedsocket.
Beforeturnonthepower,alwayscheckifthemainsvoltageisconsistent
withtheinstrumentrequired.Ensuretheratedloadofsocketisnotlowerthan
required.
IftheA.C.powerlineisdamaged,itmustbereplacedwithanewone.The

powerlineshouldbefreeofheavyobjectsduringtheinstrument’soperation.
Keepthepowerlineawayfromtheplacewherepeoplegatherregularly.
Whileconnectordisconnectthepowerline,youshouldholdthe3-pinplug
withyourhand.Inserttheplugthoroughlytoensuregoodcontactbetweenthe
plugandsocket.Pulltheplug,nottheline,whenyouneedtodisconnecttothe
mains.
Duringinstrumentoperation,themetalpartandthehotlidcanreachhigh
temperature,maycauseburnsandliquidboilover，isnotallowedtobetouched
byanypartofthebodyforfearofthebodyburn,duringthewholeoperation.
Warning
War ning
Warning
WarningSign
Sig n
Sign
Sign
Theinstrumentshouldbeplacedinalow-humidity,dust-free,and
good-ventilationroomwithoutcausticgasorpowerfulmagneticinterference.In
addition,watersources,suchaspoolsandwaterpipes,shouldbeseparateda
distancefromtheinstrument.Donotplacethedeviceinadampanddusty
place.
Nevercoverorobstructtheopeningsoftheinstrument,whicharedesigned
forventilationandtopreventthedevice’sinteriorfrombeingtoohot.
Iftheinstrumentissetasideforalongtime,itisrecommendedtodisconnect
thepowerlinetomainsandcoverthedevicewithapieceofsoftclothorplastic
topreventdustfromentering.
Onceoneofthefollowingeventsoccurs,youaresuggestedtodisconnect
thepowerlinewithmains,andcontactthedistributororaskacertificated
maintenanceworkerforhelp：
●Liquidintothedevice;
●Thedevicesprinkledordrenched;
●Thedevicemalfunctioning,givingoffabnormalsoundorodor,orthe
screendisplaysError!
●Thedevicefallingontothefloororitsshelldamaged;
●Significantchangesinthedevice’sperformance.
HOT
HOT
HOT
HOTSURFACE!
SU RFACE!
SURFACE!
SURFACE!

3.
3.
3.
3.Maintenance
Ma intenance
Maintenance
Maintenance
Theconicalholesovertheblockandthehotlidshouldbecleanedtermlywithsoaked
cottonswabwithalittleabsolutealcohol.Thatensuressufficientcontactandthusgoodheat
conductionbetweeneachconicalholeandthetubeinsideit.
Incaseitissmeared,thesurfaceoftheinstrumentmaybescrubbedwithapieceofsoftcloth
withsuitabledetergent.
Duringsurfacecleaning,thepowermustbeoff.
Strictlyprohibitdroppingdetergentintotheconicalholesovertheblock.
Corrosivescourisnotallowedtocleanthesurface.
4.
4.
4.
4.Service
Se rvice
Service
ServiceInformation
In formation
Information
Information
a)
a)
a)
a)Warranty
War ranty
Warranty
Warranty
Theinstrumentiswarrantedforaperiodofonemonth,fromdateofshipmentfromthe
company. To befreefromdefectsinmaterialandworkmanship,ourcompanyshallbe
obligated,toexchangetheinstrumentwhichprovestobedefectiveasdescribedherein.
Theinstrumentisalsowarrantedforaperiodoftwelvemonths,fromdateofshipment
fromthecompany. To befreefromdefectsinmaterialandworkmanship,ourcompany ’ s
obligationshallbelimitedtorepairorexchange(atourcompany ’ soption)oftheinstrument
whichprovestobedefectiveasdescribedherein.
Thebuyerisresponsibleforfreighttothemaintenanceshopdesignatedbyusonall
warrantyclaims.Thebuyerisalsoresponsibleforthetransportationexpensesofthefreightto
themaintenanceshop.Ourcompanyshallberesponsibleforthetransportationexpensesof
thefreighttothebuyer.
Afterthewarrantycomesdue,ourcompanyporoperlychargecostpriceformaintenance
ofadefectivedevice.
b)
b)
b)
b)Warranty
War ranty
Warranty
WarrantyTerms
Ter ms
Terms
Terms
Theabovewarrantyisnotapplicabletodefectivedeviceswithincorrectuse,abnormal
operatingconditions,improperapplication,andunauthorizedmaintenanceoralteration.

CONTENTS
CONTENTS
CONTENTS
CONTENTS
CHAPTER
CHAPTER
CHAPTER
CHAPTER1
1
1
1INTRODUCTION
IN TRODUCTION
INTRODUCTION
INTRODUCTION……………………………………………………………………………
…… ………………………………………………………………………
……………………………………………………………………………
……………………………………………………………………………
1
1
1
1
1
1
1
1PCR
PCR
PCR
PCRTechnique
Te chnique
Technique
Technique………………………………………………………………………………………
…… …………………………………………………………………………………
………………………………………………………………………………………
………………………………………………………………………………………
1
1
1
1
2
2
2
2PCR
PCR
PCR
PCRApplication
Ap plication
Application
Applicationand
an d
and
andApply
Ap ply
Apply
ApplyRange
Ra nge
Range
Range………………………………………………………………
…… …………………………………………………………
………………………………………………………………
………………………………………………………………
1
1
1
1
3
3
3
3Features
Features
Features
Features……………………………………………………………………………………………
…… ………………………………………………………………………………………
……………………………………………………………………………………………
……………………………………………………………………………………………
1
1
1
1
CHAPTER
CHAPTER
CHAPTER
CHAPTER2
2
2
2SPECIFICATIONS
SP ECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS…………………………………………………………………………
…… ……………………………………………………………………
…………………………………………………………………………
…………………………………………………………………………
2
2
2
2
1
1
1
1Normal
Normal
Normal
NormalOperating
Op erating
Operating
OperatingConditions
Co nditions
Conditions
Conditions…………………………………………………………………
…… ……………………………………………………………
…………………………………………………………………
…………………………………………………………………
2
2
2
2
2
2
2
2Transportation
Transportation
Transportation
Transportationand
an d
and
andStorage
St orage
Storage
StorageCondition
Co ndition
Condition
Condition………………………………………………………
…… …………………………………………………
………………………………………………………
………………………………………………………
2
2
2
2
3
3
3
3Basic
Basic
Basic
BasicParameters
Pa rameters
Parameters
Parameters…………………………………………………………………………………
…… ……………………………………………………………………………
…………………………………………………………………………………
…………………………………………………………………………………
2
2
2
2
4
4
4
4Software
Software
Software
SoftwareFunction
Fu nction
Function
Function…………………………………………………………………………………
…… ……………………………………………………………………………
…………………………………………………………………………………
…………………………………………………………………………………
2
2
2
2
5
5
5
5Structure
Structure
Structure
Structureand
an d
and
andOperation
Op eration
Operation
OperationPanel
Pa nel
Panel
Panel…………………………………………………………………
…… ……………………………………………………………
…………………………………………………………………
…………………………………………………………………
3
3
3
3
6.Replacement
6.Replacement
6.Replacement
6.Replacementof
of
o f
ofthe
th e
the
themodule
mo dule
module
module………………………………………………………………………
……… ………………………………………………………………
………………………………………………………………………
………………………………………………………………………6
6
6
6
CHAPTER
CHAPTER
CHAPTER
CHAPTER3
3
3
3PREPARATIONS
PR EPARATIONS
PREPARATIONS
PREPARATIONS……………………………………………………………………………
…… ………………………………………………………………………
……………………………………………………………………………
……………………………………………………………………………
8
8
8
8
1
1
1
1Inspection
Inspection
Inspection
InspectionBefore
Be fore
Before
BeforePower
Po wer
Power
PowerOn
On
O n
On……………………………………………………………………
…… ………………………………………………………………
……………………………………………………………………
……………………………………………………………………
8
8
8
8
2
2
2
2Power-On
Power-On
Power-On
Power-On……………………………………………………………………………………………
…… ………………………………………………………………………………………
……………………………………………………………………………………………
……………………………………………………………………………………………
8
8
8
8
3
3
3
3Hotlid
Hotlid
Hotlid
HotlidInspection
In spection
Inspection
Inspection…………………………………………………………………………………
…… ……………………………………………………………………………
…………………………………………………………………………………
…………………………………………………………………………………
8
8
8
8
CHAPTER
CHAPTER
CHAPTER
CHAPTER4
4
4
4OPERATION
OP ERATION
OPERATION
OPERATIONGUIDE
GU IDE
GUIDE
GUIDE…………………………………………………………………………
…… ……………………………………………………………………
…………………………………………………………………………
…………………………………………………………………………
9
9
9
9
1
1
1
1Edit
Edit
Edit
Edita
a
a
aNew
Ne w
New
NewPCR
PC R
PCR
PCRAmplification
Am plification
Amplification
AmplificationFile
Fi le
File
File……………………………………………………………
…… ………………………………………………………
……………………………………………………………
……………………………………………………………
9
9
9
9

2
2
2
2Access
Ac cess
Access
Accessan
an
a n
anPCR
PC R
PCR
PCRFile
Fi le
File
File………………………………………………………………………………
……… ………………………………………………………………………
………………………………………………………………………………
………………………………………………………………………………15
15
1 5
15
3
3
3
3Modify
Modify
Modify
Modifyan
an
a n
anPCR
PC R
PCR
PCRFile
Fi le
File
File………………………………………………………………………………
……… ………………………………………………………………………
………………………………………………………………………………
………………………………………………………………………………16
16
1 6
16
4
4
4
4Delete
Delete
Delete
Deletea
a
a
aFile
Fi le
File
File……………………………………………………………………………………
……… ……………………………………………………………………………
……………………………………………………………………………………
……………………………………………………………………………………17
17
1 7
17
5
5
5
5Run
Run
Run
Runa
a
a
aPCR
PC R
PCR
PCRProgram
Pr ogram
Program
Program………………………………………………………………………………
……… ………………………………………………………………………
………………………………………………………………………………
………………………………………………………………………………18
18
1 8
18
6
6
6
6Translate
Translate
Translate
Translatethe
th e
the
theDisplay
Di splay
Display
DisplayDuring
Du ring
During
DuringProgram
Pr ogram
Program
ProgramExecution
Ex ecution
Execution
Execution………………………………………
……… ………………………………
………………………………………
………………………………………21
21
2 1
21
7
7
7
7Access
Access
Access
Accessand
an d
and
andCalibrate
Ca librate
Calibrate
Calibratethe
th e
the
theInternal
In ternal
Internal
InternalClock
Cl ock
Clock
Clock…………………………………………………
……… …………………………………………
…………………………………………………
…………………………………………………22
22
2 2
22
CHAPTER
CHAPTER
CHAPTER
CHAPTER5
5
5
5MAINTENANCE
MA INTENANCE
MAINTENANCE
MAINTENANCEAND
AN D
AND
ANDFAILURE
FA ILURE
FAILURE
FAILUREANALYSIS
AN ALYSIS
ANALYSIS
ANALYSIS…………………………………………………
……… …………………………………………
…………………………………………………
…………………………………………………23
23
2 3
23
1.
1.
1.
1.Maintenance
Ma intenance
Maintenance
Maintenance……………………………………………………………………………………
……… ……………………………………………………………………………
……………………………………………………………………………………
……………………………………………………………………………………23
23
2 3
23
2.
2.
2.
2.Possible
Po ssible
Possible
PossibleCause
Ca use
Cause
Causeof
of
o f
ofFailure
Fa ilure
Failure
Failureand
an d
and
andProcessing
Pr ocessing
Processing
ProcessingProcedure
Pr ocedure
Procedure
Procedure…………………………………
……… …………………………
…………………………………
…………………………………24
24
2 4
24
3.
3.
3.
3.Phenomenon
Ph enomenon
Phenomenon
Phenomenonof
of
o f
ofFailure
Fa ilure
Failure
Failureand
an d
and
andIts
It s
Its
ItsFailure
Fa ilure
Failure
FailureNo.
No .
No.
No.………………………………………………
……… ………………………………………
………………………………………………
………………………………………………25
25
2 5
25
APPENDIX
APPENDIX
APPENDIX
APPENDIX1
1
1
1Wiring
Wi ring
Wiring
WiringDiagram
Di agram
Diagram
Diagram………………………………………………………………………
……… ………………………………………………………………
………………………………………………………………………
………………………………………………………………………26
26
2 6
26

1
CHAPTER
CHAPTER
CHAPTER
CHAPTER1
1
1
1INTRODUCTION
IN TRODUCTION
INTRODUCTION
INTRODUCTION
1.
1.
1.
1.PCR
PCR
P CR
PCRTechnique
Tec hnique
Technique
Technique
PolymeraseChainReaction(PCR)isatechniquewhichisusedtoamplifythenumberof
copiesofaspecificregionofDNAdevelopedinmiddleof80s.Therearemanydistinctmerits
inthePCRtechnology,suchasspecialization,sensitivity,highproductivity,celerity,
convenience,goodreproductively,automatizationandsoon.Inareactiontube,targetgene
orsegmentofDNA,inamatterofhours,willbeamplifiedamillionfold,andcanbeseenby
nakedeyes;fromahair,adropofblood,evenacellcanbeamplifiedtoenoughnumberof
DNAforanalyzingandidentifying.Inthepast,theproceduremaytakedaysandweeks,but
nowitonlytakeafewhours.PCRisarevolutionaryinnovationandlandmarkinthefieldof
biologicalmedicine.
2.
2.
2.
2.Application
App lication
Application
Applicationand
and
a nd
andApply
App ly
Apply
ApplyRange
Ran ge
Range
Range
PCRhasbeenwidelyappliedinimmunology,humangenomeproject,forensics,oncology,
populationbiology,paleontology，zoology，botanyresearchfieldandclinicaldiagnosisofvirus,
tumor,hereditarydisease,etc.,byPCRdetection.
3.
3.
3.
3.Features
Fea tures
Features
Featuresof
of
o f
ofthe
the
t he
theGenius
Gen ius
Genius
GeniusThermal
The rmal
Thermal
ThermalCycler
Cyc ler
Cycler
Cycler
●Reliableandstableoperation,becausethethermoelectricmoduleismanufacturedviathe
ITI ’ sthermoelectriccoolingtechnique.
●Amplificationsectionisdesignedlefttotheoperationsection,thisleft-rightstructureprovide
userconvenientandsafe.
●Electricalconnectionsofmodulesaredesignedplug-to-plug，actuallymaketheexchange
wirelessly,theexchangeisconvenientandflexible.
●Highheating/cooling(heating/coolingrate≥2.5℃/sec)
●Automatictemperaturerampcontrol,whichallowsdifferentheating/coolingrampsettings.
●Unitizedprogramdesign,makeeditingacomplicatedPCRAmplificationprogramsimple.
●Thesoakmodeatatemperatureupto4℃afternormalcyclingprogramexecution.
●Instantinspectionoftotalexecutiontimeandremainingexecutiontime.
●Heatedlid,preventingthesampleinacentrifugaltubefromevaporation,whichmakes
paraffinoilunnecessary.
●Auto-restartincaseofpowerfailure,restoringthedatabeforeinterruptingandcontinuingto
performthepreviouslyinterruptedprogram.

2
CHAPTER
CHAPTER
CHAPTER
CHAPTER2
2
2
2SPECIFICATIONS
SPECI FICATIONS
SPECIFICATIONS
SPECIFICATIONS
1.
1.
1.
1.Normal
Normal
Normal
N ormalOperating
Operating
Oper ating
OperatingConditions
Conditions
C onditions
Conditions
Ambienttemperature:10℃～30℃
Relativehumidity:≤70%
Powersupply:AC220V±22V250VA50Hz±1Hz
2.
2.
2.
2.Transportation
Transportat ion
Transportation
Transportationand
and
and
andStorage
Sto rage
Sto rage
StorageConditions
Conditions
C onditions
Conditions
Ambienttemperature:-20℃～+55℃
Relativehumidity:≤80%
3.
3.
3.
3.Basic
Basic
Basic
B asicParameters
Parameters
P arameters
Parameters
Model
Parameters%ORFN[2SWLRQDO%ORFN[
PCR-3003&5
SampleNumber6436
TubeVolume(ml)0.20.5
Size(mm)
(L×W×H)
370×248.5×222
Weight(kg)4.8
Fuse250V2.5AΦ5×20
Temperaturerange:0℃～99℃
Heatingrate:≥2.5℃/s
Coolingrate:≥2.5℃/s
Temperaturedisplayaccuracy:0.1℃
Temperatureuniformity:95℃≤±0.5℃（after20sec.）
20℃～72℃≤±0.3℃（after20sec.）
Maximumnumberofprogramunits:9
Maximumprocedureswithinacycle:9
Maximumnumberofcycles:99
Maximumtemperaturekeepingtime:99min.99sec.
Maximumincubationandinsitutime:99hour99min.
Numberofprograms:100
4.
4.
4.
4.Software
Software
Sof tware
SoftwareFunctions
Functions
F unctions
Functions
Fileeditingandsaving
Fileaccessing,modifyinganddeleting
Heating/coolingrampsetting
Instantlydisplayingthedataateachphaseofprogramexecution

3
Pauseofprogramexecution
Stopofprogramexecution
Auto-restartincaseofpowerfailure
Soundalarm/indicatorlight
Estimatingtotalprogramexecutiontimeandremainingprogramexecutiontime
Dateandtimedisplayandcalibration
Protectingthedevicefromfurtherdamageandalarmingincaseoffailure
5.
5.
5.
5.Structure
Structure
S tructure
Structureand
and
and
andoperation
ope ration
o peration
operationpanel
panel
panel
p anel
Structure
Structure
Structure
Structurediagram
diagram
diag ram
diagram：
：
：
：
HeatedLid
LCDDisplay
OperationPanel
Module
Press-Button
PowerSwitch
Reserveddatacableinterface
Indicator
Fusesocket
PowerConnecter
Knob