Mj research Ptc-0200 User manual

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

@@@@@



ÀÀÀÀÀ

;;;;;

QQQQQ

¢¢¢¢¢

PTC-0200 DNA Engine &

PTC-0225 DNA Engine Tetrad

Operations Manual

Version 4.0

MJR

ESEARCH

PTC-200

PeltierThermalCycler

DNAE

ngine

Power

Instant Incubate

BlockDisplay

B

A

Block Status

Heating

Cooling

CAUTION: Sample block(s) may be very hot !

ATTENTION: Les blocs peuvent erte tres chauds !

Pause

Stop

Cancel

Proceed

Select

DNA Engine T

etrad

CAUTION:Sampleblocksmaybeveryhot!

ATTENTION:Lesblocspeuventêtretrèschauds!

Proceed

Cancel

Select

Power

PTC-225

Peltier Thermal Cycler

MJ

R

ESEARCH

Block Status

B

2

A

B

1

A

4

B

A

3

B

Heating Cooling

Instant

Block

1

4

7

2

5

8

3

6

90

Pause

Stop

M J R e s e a r c h

05434-05

PTC-0200 DNA Engine &

PTC-0225 DNA Engine Tetrad

Operations Manual

Version 4.0Version 4.0

Version 4.0

MJ Research, Inc.

Waltham, Massachusetts

electronic, is prohi ited without written permission of MJ Research, Inc.

DNA Engine, DNA Engine Tetrad, Alpha, Hot Bonnet, Power Bonnet, Microseal, Chill-Out, Self-

Seal and Twin Tower are trademarks of MJ Research, Inc.

Thermal cyclers can e used for a num er of purposes, including the polymerase chain reaction

(PCR). PCR is covered y patents owned y Hoffmann-La Roche, Inc., and F. Hoffmann-La Roche,

Ltd., who have granted exclusive and nonexclusive licenses for some types of applications. Roche

and its licensees provide end-user licenses within their respective fields. These licenses have dif-

ferent terms depending on the particular application of PCR, and different rules may apply in

different countries. Anyone who intends to use MJ Research equipment to do PCR is encouraged

to contact Hoffmann-La Roche for more information, at one of the addresses elow:

In the United States: In other nations:

Kathe Kiehn Dr. Agnieszka Junosza-Jankowski

Licensing Manager PCR Licensing Manager

Roche Molecular Systems, Inc. F. Hoffmann-La Roche Ltd.

1145 Atlantic Avenue Building 222/350

Alameda, CA 94501 USA CH-4002 Basel, Switzerland

(510) 814-2970 41-61-687-3031

Fax: (510) 814-2977 Fax: 41-61-687-2113

05434-05

Documentation Conventions ............................................................................................... iv

Part I: The DNA Engine

1. Introduction ................................................................................................................. 1-1

2. Layout and Specifications .......................................................................................... 2-1

3. Installation.................................................................................................................... 3-1

4. Operation...................................................................................................................... 4-1

5. Running Protocols ....................................................................................................... 5-1

6. Creating Programs ...................................................................................................... 6-1

7. Editing Programs ........................................................................................................ 7-1

8. Using the Utilities........................................................................................................ 8-1

9. Networking ..................................................................................................................9-1

10. Maintenance ................................................................................................................. 10-1

11. Trou leshooting ........................................................................................................... 11-1

Part II: The DNA Engine Tetrad

12. Information Specific to the DNA Engine Tetrad..................................................... 12-1

Part III: Accessories

13. The Remote Alpha Dock System .............................................................................. 13-1

Appendix A: Safety Warnings and Guidelines ............................................................. A-1

Appendix B: How a Peltier Heat Pump Works ............................................................. B-1

Appendix C: How a Switching Power Supply Works ................................................. C-1

Appendix D: Shipping Instructions for US Residents.................................................. D-1

Appendix E: Warranties .................................................................................................... E-1

Appendix F: Factory-Installed Protocols........................................................................ F-1

Index ..................................................................................................................................... In-1

Contents

iviv

iv

Documentation Conventions

T pographic Conventions

The names of key oard keys are set in sans serif type and encased in

dou le angle rackets:

ExampleExample

Example «Proceed»

Items in programming menus are italicized:

ExampleExample

Example Select Edit from the Main Menu.

Graphic Conventions

The programming screens displayed in the LCD window are represented

y a ox containing four lines of text:

ExampleExample

Example

Terminolog

A programming option is termed “selected” when the cursor is positioned

in front of it. Use the «Select» keys (see fig. 2-2) to move the cursor. In

some screens selected items are also displayed in all-capital letters.

Run: 2-STEP

1= 92.0 for 0:05

Cycle: 1

Calc: 65.0

Part I

The DNA Engine

1-11-1

1-1

Introduction

Meet the DNA Engine, 1-2

Using This Manual, 1-2

Important Safety Information, 1-3

1

1-21-2

1-2

DNA Engine & DNA Engine Tetrad Operations Manual

Meet the DNA Engine

Thank you for purchasing an MJ RESEAR H PT -200 DNA Engine. De-

signed by a team of molecular biologists and engineers, the DNA Engine

will meet your needs for a versatile, easy-to-use, reliable, and compact

programmable thermal cycler:

• Interchangeable sample blocks—the Alpha unit family— that accom-

modate many types of tubes, microplates, and slides

• Hot Bonnet heated lid (or its remote-controlled version, the Power Bon-

net) for oil-free cycling

• Intuitive software with easy-to-read interface for quick and painless

programming, editing, file management, password protection, and

much more

• hoice of calculated sample temperature control for highest speed

and accuracy, or block or probe control for compatibility with proto-

cols designed for a variety of instrument types

• Space-saving design for easy setup and transportation

• Instant Incubate feature for continuous-temperature incubations

• Networking for up to 15 machines, for convenient remote operation

and documentation of runs

• ustomizable factory-installed protocols

When multiple users or high-throughput operations must be accommo-

dated, the PT -225 DNA Engine Tetrad is available. Essentially consist-

ing of four DNA Engines placed in one chassis, the DNA Engine Tetrad

has the same features and ease of use of the stand-alone DNA Engine.

Using This Manual

This manual contains all the information you need to operate your DNA

Engine or DNA Engine Tetrad safely and productively:

• hapter 2 acquaints you with the physical characteristics of the DNA

Engine.

• hapters 3–5 present the basics of installing and operating the DNA

Engine.

• hapters 6 and 7 describe programming the DNA Engine.

• hapter 8 outlines the utilities available for the DNA Engine.

• hapter 9 describes how to network and remotely operate the DNA

Engine and DNA Engine Tetrad.

1-31-3

1-3

Introduction

• hapter 10 explains the proper maintenance of the DNA Engine.

• hapter 11 offers troubleshooting information for the DNA Engine.

• hapter 12 describes the operation of the DNA Engine Tetrad.

• hapter 13 describes the operation of the RAD–0200 Remote Alpha

Dock accessory.

Important Safety Information

Safe operation of the DNA Engine or DNA Engine Tetrad begins with a

complete understanding of how the machine works. Please read this en-

tire manual before attempting to operate the DNA Engine or DNA En-

gine Tetrad. Do not allow anyone who has not read this manual to oper-

ate the machine.

The DNA Engine and DNA Engine Tetrad can generate enough heat to

inflict serious burns and can deliver strong electrical shocks if not used

according to the instructions in this manual. Please read the safety warn-

ings and guidelines in appendix A, and exercise all precautions outlined

in them.

2-12-1

2-1

Layout and Specifications

Front View, 2-2

Control Panel, 2-2

Back View, 2-3

Bottom View, 2-3

Alpha Units, 2-4

Single-Block Models, 2-4

Dual-Block Models, 2-4

Slide Block, 2-4

Power Bonnet Accessor , 2-4

Specifications, 2-5

Gradient Specifications, 2-6

2

2-22-2

2-2

DNA Engine & DNA Engine Tetrad Operations Manual

Front View

(Figure 2-1)

Control Panel

(Figure 2-2)

Air exhaust vents

(also on other side)

MJ R

ESEARCH

PTC-200

Peltier Thermal Cycler

DNA E

ngine

Power

Instant Incubate

Block Display

B

A

Block Status

Heating

Cooling

CAUTION: Sample block(s) may be very hot !

ATTENTION: Les blocs peuvent erte tres chauds !

Pause

Stop

Cancel

Proceed

Select

Alpha unit, lid losed

Air intake vents

Control panel

Thumbwheel

DNA Engine

Select

3

12

456

789

.0–

Proceed

Cancel

Stop

Pause

Power

BlockInstant Incubate

AB

Block Status

Heating

Cooling

CAUTION:Sample blocks may be very hot !

ATTENTION: Les blocs peuvent etre tres chauds !

`

^

MJ RESEARCH

PTC-200

Peltier Thermal Cycler

Blo k status lights

Blo k key

LCD window

Left and right

sele tion keys

Instant in ubation key

Can el key

Pro eed key

Power light

2-32-3

2-3

Layout and Spe ifi ations

Back View

(Figure 2- )

Bottom View

(Figure 2-4)

Air intake vents

Power ord ja k

Alpha unit handle

Alpha unit Air exhaust vents

(also on other side)

IEEE-488 port

Parallel printer port

RS-232 port

Ba k Front

Air intake vents

2-42-4

2-4

DNA Engine & DNA Engine Tetrad Operations Manual

Alpha Units

Single-Block Models

60V Alpha: Holds 60 x 0.5ml tubes

96V Alpha: Holds 96 x 0.2ml tubes or one 96-well

microplate

192 Alpha: Holds one 192-well microplate

384 Alpha: Holds one 384-well microplate or

one 96-well microplate

Dual-Block Models

30/30 Dual Alpha: Holds 2 x 30 x 0.5ml tubes

30/48 Dual Alpha: Holds 1 x 30 x 0.5ml tubes

and 1 x 48 x 0.2ml tubes

48/48 Dual Alpha: Holds 2 x 48 x 0.2ml tubes or

half plates

Slide Block

Twin Tower: Holds 2 x 16 standard slides

Power Bonnet Accessory

Permits remote control of Alpha unit lid opening; avail-

able for Alpha models 96V and 384.

2-52-5

2-5

Layout and Spe ifi ations

Specifications

Thermal range: –5˚ to 105˚C, but no more than 30˚C below ambient

temperature

Accuracy: ±0.3˚C of programmed target @ 90˚C, NIST-traceable

Thermal homogeneity: ±0.4˚C well-to-well within 30 seconds of arrival at

90˚C (for most Alpha units; see specifications for in-

dividual Alpha units)

Ramping speed: Up to 3˚C/sec for all single- and dual-block Alpha

units; 1.2˚C/sec for the Twin Tower

Sample capacity: Varies with installed Alpha unit

Line voltage: 100–240VAC rms (no adjustment needed among volt-

ages within these ranges)

Frequency: 50–60Hz single phase

Power: 850W maximum

Fuses: Two 6.3A, 250V, 5 x 20mm

Displays: One 20 x 4 LCD alphanumeric displa

Ports: One 25-pin 8-bit parallel interface printer port

One 9-pin RS-232 serial port for printer or remote use

One IEEE-488 bidirectional general purpose interface

bus

Memory: 200 t pical programs in up to 12 individual folders

eight: 7.6–9.3kg, varies with installed Alpha unit (DNA

Engine Tetrad: 29kg, including Alpha units and

power suppl )

Size: 24 x 35 x 17cm (DNA Engine Tetrad: 37 x 55 x 25cm)

Gradient Specifications (96V Alpha module only)

Accurac : + 0.4°C of programmed target at end columns, 30

seconds after the timer starts for the gradient step,

NIST–traceable

Column uniformit : + 0.4°C , well–to–well within column, within 30 sec-

onds of reaching target temperature

Calculator accurac : + 0.4°C of actual well temperature

Lowest programmable 30°C

temperature

Highest programmable 105°C

temperature

Temperature differential 1–24°C

range

3-13-1

3-1

Installation

Packing Checklist, 3-2

Setting Up the DNA Engine, 3-2

Environmental Requirements, 3-2

Power Supply Requirements, 3-3

Air Supply Requirements, 3-3

Ensuring an A equate Air Supply, 3-3

Ensuring That Air Is Cool Enough, 3-4

Requirements for Robotics Installations, 3-5

3

3-23-2

3-2

DNA Engine & DNA Engine Tetrad Operations Manual

Packing Checklist

After unpacking the DNA Engine, check to see that you have receive

the following:

• One DNA Engine base

• One Alpha unit (more if a itional units were or ere )

• Two spare fuses

• One power cor

• One probe, if purchase

•PTC-200 DNA Engine & PTC-225 DNA Engine Tetrad Operations Manual

(this ocument)

• Pro uct registration car (US customers only)

• Exten e warranty application (US customers only)

• DNA Engine Driver remote software request form

If any of these components are missing or amage , contact MJ RESEARCH

or the authorize istributor from whom you purchase the DNA En-

gine to obtain a replacement. Please save the original packing materials

in case you nee to return the DNA Engine for service. See appen ix E

for shipping instructions.

Setting Up the DNA Engine

The DNA Engine requires only minimal assembly: plugging in the power

cor an inserting an Alpha unit. Insert the power cor plug into its jack

at the back of the machine (see fig. 2-3 for location of jack), then plug the

cor into an electrical outlet. With the machine turne off, insert an Alpha

unit (see “Installing an Alpha Unit,” chapter 4).

Caution:Caution:

Caution: Do not insert or remove an Alpha unit with the DNA

Engine turne on; electrical arcing can result. Rea the

safety warning in appen ix A regar ing electrical safety

when inserting or removing an Alpha unit.

Environmental Requirements

Ensure that the area where the DNA Engine is installe meets the follow-

ing con itions, for reasons of safety an performance:

• Nonexplosive environment

• Normal air pressure (altitu e below 4000m)

3-33-3

3-3

Installation

• Ambient temperature 5˚–31˚C

• Relative humi ity up to 90%

• Unobstructe access to air that is 31˚C or cooler (see below)

• Protection from excessive heat an acci ental spills (Do not place

the DNA Engine near such heat sources as ra iators, an protect it

from anger of having water or other flui s splashe on it, which

can cause shorting in its electrical circuits.)

Power Supply Requirements

The DNA Engine requires 100–240VAC, 50–60Hz, an a groun e out-

let. The DNA Engine can use current in the specifie range without

a justment, so there is no voltage-setting switch.

Power cor s for outlets other than the US 120V outlet may be purchase

from computer stores, since they are also use for most esktop computers

an printers an meet international stan ar IEC-320. The power cor must

be rate to carry at least 10A at 125V or 250V, epen ing on the voltage

available in your nation. The quality of the power cor can be further en-

sure by making certain it is inscribe with the tra emark of UL, CSA, TUV,

VDE, or another national testing agency.

NN

Note:ote:

ote:ote:

ote: Do not cut the supplie 120V power cor an attach a ifferent con-

nector. Use a one-piece mol e connector of the type specifie above.

Air Supply Requirements

The DNA Engine requires a constant supply of air that is 31˚C or cooler in

or er to remove heat from the Alpha unit’s heat sink. Air is taken in from

vents at the front, back, an bottom of the machine an exhauste from

vents on both si es (see figs. 2-1, 2-3, an 2-4). If the air supply is ina equate

or too hot, the machine can overheat, causing performance problems, soft-

ware error messages (particularly “HS Overheating” an “Slow Block Cy-

cling”), an even automatic shut owns. Special attention shoul be pai to

airflow an air temperature in robotics installations of DNA Engines.

Ensuring an A equate Air Supply

• Do not block the air intake vents.

Position the DNA Engine at least 10cm from vertical surfaces an

other thermal cyclers (greater istances may be require ; see be-

low). Do not put loose papers un er the machine; they can be sucke

into the air intake vents on the bottom of the machine.

MJ Research PTC-0200 User manual

Popular Engine manuals by other brands