MMM CO2 Series User manual

Instructions for use
CO2 INCUBATOR
CO2Cell 190 Comfort
CO2Cell 190S Comfort
(with sterilization 200 °C)
CO2CELL Comfort 190_np_en 1301_mmm_V1.02

CONTENTS:
24. 1. 2013
1SAFETY ......................................................................................................................................................3
1.1 LEGAL CONSIDERATIONS ............................................................................................................................3
1.2 INTENDED USE .........................................................................................................................................3
1.3 SYMBOLS USED IN THIS MANUAL..................................................................................................................3
1.4 GENERAL SAFETY INSTRUCTIONS.................................................................................................................3
2DESCRIPTION ............................................................................................................................................. 3
2.1 CONSTRUCTION .......................................................................................................................................4
2.2 DOORS ..................................................................................................................................................4
2.3 CONTROL SYSTEM....................................................................................................................................4
2.4 CO2SENSOR ...........................................................................................................................................4
2.5 DIAGRAMS ..............................................................................................................................................4
2.5.1 OUTER VIEW ...................................................................................................................................... 4
2.5.2 INNER VIEW ....................................................................................................................................... 4
2.5.3 REAR CONNECTIONS ............................................................................................................................ 4
3DELIVERY, TRANSPORTATION AND STORAGE...................................................................................... 5
3.1 UNPACKING.............................................................................................................................................5
3.2 LIFTING AND MOVING ................................................................................................................................5
3.3 STORAGE ...............................................................................................................................................5
4INSTALLATION............................................................................................................................................5
4.1 LOCATION AND AMBIENT CONDITIONS............................................................................................................ 5
4.2 SHELVES AND USABLE SPACE .....................................................................................................................6
4.3 WATER TRAY...........................................................................................................................................6
4.4 GAS CONNECTION ....................................................................................................................................6
4.5 ELECTRICAL CONNECTION ..........................................................................................................................6
5GETTING STARTED....................................................................................................................................7
5.1 START UP ..............................................................................................................................................7
5.2 SETTING TIME AND DATE............................................................................................................................7
5.3 FACTORY PRESETS ...................................................................................................................................7
5.4 CHANGING TEMPERATURE SET POINTS AND HIGH / LOW ALARMS ........................................................................7
5.5 CHANGING CO2SET POINT AND ALARM THRESHOLDS .......................................................................................8
5.6 ALARM STATUS SCREEN.............................................................................................................................8
6OTHER FEATURES.....................................................................................................................................9
6.1 GRAPHICAL DISPLAY .................................................................................................................................9
6.2 UTILITY MENU..........................................................................................................................................9
6.3 DOOR ALARM MENU................................................................................................................................10
6.4 INCUBATOR INFORMATION .........................................................................................................................10
6.5 HISTORICAL EVENT LOG...........................................................................................................................10
6.6 USER SECURITY..................................................................................................................................... 10
6.6.1 ENTERING AUSER NAME AND PASSWORD ............................................................................................... 10
6.6.2 ENABLING SECURITY........................................................................................................................... 10
6.6.3 LOGGING OUT ....................................................................................................................................11
6.6.4 DISABLING SECURITY ...........................................................................................................................11
7SD CARD DATA LOGGING....................................................................................................................... 11

7.1 START LOGGING..................................................................................................................................... 11
7.2 CHANGING FILENAME ..............................................................................................................................12
7.3 REMOVING THE SD CARD .........................................................................................................................12
8HIGH TEMPERATURE STERILISATION .................................................................................................. 13
8.1 PREPARATION........................................................................................................................................ 13
8.2 STARTING THE CYCLE..............................................................................................................................13
8.3 STERILISE CYCLE ...................................................................................................................................13
8.4 COOLING PHASE ....................................................................................................................................14
8.5 ABORTING THE STERILISE CYCLE ...............................................................................................................14
9ERROR MESSAGE SCREENS ................................................................................................................. 14
10 OPTIONS ..................................................................................................................................................15
10.1 VOLT FREE ALARM RELAY (BMS)................................................................................................................15
10.2 4-20 MA RETRANSMISSION (RETX)..............................................................................................................15
10.3 RS232 SERIAL COMMUNICATION (232)........................................................................................................15
10.4 ETHERNET COMMUNICATION (ETH).............................................................................................................15
10.5 MULTI-DOOR (8ID)...................................................................................................................................15
11 ACCESSORIES .........................................................................................................................................15
12 CLEANING.................................................................................................................................................16
12.1 OUTER SURFACES ..................................................................................................................................16
12.2 INNER SURFACES....................................................................................................................................16
12.3 OXIDATION AT HIGH TEMPERATURE (COMFORT 190SONLY).............................................................................. 16
13 SERVICE....................................................................................................................................................16
13.1 WARRANTY...........................................................................................................................................16
13.2 PREVENTATIVE MAINTENANCE ...................................................................................................................16
13.3 RETURNING AUNIT .................................................................................................................................16
14 DISPOSAL .................................................................................................................................................17
15 TECHNICAL INFORMATION......................................................................................................................17
15.1 TECHNICAL DATA ....................................................................................................................................17
15.2 DIMENSIONAL DRAWINGS .........................................................................................................................17

Instructions for use
CO2CELL Comfort 190_np_en 1301_mmm_V1.02 3
1 SAFETY
1.1 LEGAL CONSIDERATIONS
It is important that you read this manual
completely and observe all instructions.
Failure to follow these instructions may result
in personal injury, equipment damage and
incorrect performance of the incubator.
This manual contains information for installing, operation
and maintenance of the incubator. It cannot cover all
possible uses and applications. Please contact your dealer
or MMM directly if you have a special application or the
manual does not describe your problem in enough detail.
The contents of this manual and the incubator are subject to
change without notice.
1.2 INTENDED USE
The CO2Cell Comfort 190 incubators are intended for
mammalian cell cultivation under typical conditions of
37.0 °C. The pH of the cell media can be controlled by
altering the CO2concentration within the incubator.
Other applications are not approved by MMM. Please
contact your dealer or MMM directly, for advice if you intend
to use this incubator for other applications.
1.3 SYMBOLS USED IN THIS MANUAL
The following symbols are used throughout this manual:
Risk of injury Electrical
hazard
Hot surface Lifting
hazard
Stability
hazard
Compressed
gas hazard
Asphyxiation
hazard
Mandatory
regulation
Read
operating
manual
Lift with
several
persons
Disconnect
from
electrical
supply
Important
information
for correct
operation
1.4 GENERAL SAFETY INSTRUCTIONS
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laboratory personnel who are familiar with all precautions
and standard safety practices for working in a laboratory
environment.
These safety instructions are intended to be used alongside
any local safety guidelines, rules and regulations. If in
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organisation.
Only skilled electricians or engineers authorised by MMM
can perform repairs or maintenance on this incubator. Only
original spare parts from MMM can be used. There are no
user serviceable parts within the incubator.
MMM takes no responsibility for 3rd party accessories or
equipment used with this incubator.
DANGER
Electrical hazard
Danger of death
Do not remove any panels from incubator.
Do not allow the incubator to become wet.
HOT
SURFACES
Danger of burning
Risk of injury
The glass door and inner chamber reach
200 °C during sterilisation.
Do not force the door open during
sterilisation.
STABILITY
HAZARD
Stability hazard
Risk of injury
Damage to unit
Do not place the unit too close to the edge
of a bench.
Do not lean on the door when open.
LIFTING
HAZARD
Lifting hazard
Risk of injury
Damage to unit
Lift with 4 people
95 kg
Observe safe handling regulations
2 DESCRIPTION
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generation of programmable controller, ensuring precise
control of temperature and CO2levels.
Accurate and stable heating throughout the entire chamber
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heating element. In addition a separate independently
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door is free from condensation.
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convection of the air within the chamber, ensuring even
temperature and gas distribution throughout the chamber.
CO2Cell Comfort incubator has extra heaters that allow
high temperature 200 °C dry sterilisation of the incubator
chamber.

Instructions for use
4CO2CELL Comfort 190_np_en 1301_mmm_V1.02
2.1 CONSTRUCTION
The inner chamber is made from 316 grade stainless steel.
It is deep drawn from one piece with no welds and is
electro-polished, meaning that the inner surfaces are
smooth and easily cleaned.
The removable shelves, racking and water tray are also
made from stainless steel.
The outer housing is made from stainless steel and powder
coated with anti-bacterial biocide paint.
2.2 DOORS
The outer door has a heater on its inner face. This door is
an integral part of the temperature control system and must
be closed for the incubator to function normally.
The door is hinged on the right hand side. Left hand opening
is only available as an option at the point of ordering, retro-
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When this door is open, the CO2valve is closed and
heater power is reduced to prevent over shoot of operating
conditions. The door is linked to the controller alarm system.
DSection 6.3
On the Comfort 190S incubator, 2 magnetic solenoid
locks prevent the outer door from being opened while the
incubator is in a high temperature sterilise cycle.
An inner glass door seals against a thermoplastic elastomer
gasket. This allows viewing of the samples within the
chamber without disturbing the temperature or atmosphere
of the chamber.
The incubator can be optionally equipped with a multi-door.
These multi-doors improve temperature and CO2recovery
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DSection 10.
2.3 CONTROL SYSTEM
The colour LCD touch screen display allows the user to
easily see operating conditions and to alter settings through
icon based menus.
Software controlled PID loops constantly monitor
temperature and CO2values, making automatic adjustments
to keep the chamber atmosphere within limits.
The controller is equipped with a built in feature that logs
incubator data directly to a removable SD card. This
data is in Excel format, making it easy to view with a PC.
DSection 7.
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to connect the incubator to a PC so that you can remotely
access the SD card data. PC software is required to do this.
Contact your dealer or MMM directly for advice.
Other optional communication options are Ethernet PC
interface and 4-20 mA retransmit. DSection 10.
2.4 CO2SENSOR
CO2is measured and controlled using a highly accurate
infra-red sensor. This sensor remains practically drift free
between calibration periods.
The sensor is not mounted within the chamber; instead a
small pump passes the chamber atmosphere through the
sensor.
The sensor does not have to be removed during high
temperature sterilisation.
2.5 DIAGRAMS
2.5.1 OUTER VIEW
2.5.2 INNER VIEW
2.5.3 REAR CONNECTIONS

Instructions for use
CO2CELL Comfort 190_np_en 1301_mmm_V1.02 5
3 DELIVERY,
TRANSPORTATION
AND STORAGE
3.1 UNPACKING
Inspect the packaging for signs of transportation damage.
Carefully undo the top of the cardboard packaging and
gently slide the packaging over the incubator.
Inspect the incubator and inform the carrier immediately if
there are any signs of transportation damage.
Remove any packaging material from within the chamber.
Remove any protective coverings from the surfaces of the
inner chamber.
Remove the operating manual and accessory kit from within
the chamber. Keep these safe.
Please check that the accessory pack contains the following
items. Contact your dealer with details of any shortages.
Comfort 190 accessory pack Qty
Stainless steel perforated shelves 4
Shelf racking (left and right hand) 1 set
Racking support bar 1
Stainless steel water tray 1
Service port bungs 2
Formatted 2Gb SD card 1
Instructions for use 1
3.5 meter length of CO2tubing with HEPA
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1
Hose clips 2
Test report 1
IEC mains power lead 1
3.2 LIFTING AND MOVING
These incubators are heavy. The weights are as follows:
Comfort 190 is 94,5 Kg
Comfort 190S is 104 Kg
Please refer to Manual Handling Policy of your organisation
before attempting to lift the incubators.
Risk of unit sliding or tipping.
Risk of injury by lifting heavy loads.
Risk of damage to the unit.
Always lift using the handles on the outside
of the incubator.
Lift with 4 people.
Do not lift by using the door.
3.3 STORAGE
If the incubator has been in a cold location for a
period then it needs to acclimatise to room
temperature.
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before start up. This will allow any condensation that may
have formed to dry. It will also stop the incubator from
overshooting temperature set point.
4 INSTALLATION
4.1 LOCATION AND AMBIENT
CONDITIONS
The incubator can be placed on a table or worktop.
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enough to carry the weight of the incubator.
MMM do not recommend placing the incubator directly on
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Wheeled platform forms are available that lift the incubator
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using the stacking kit. DSection 10.
Correct location of an incubator is very important. The
incubator is designed to work in a typical laboratory
environment.
For good performance the ambient conditions should be at
least 6 °C below the working temperature.
E.g. for a set point of 37.0 °C the ambient temperature must
not exceed 31.0 °C.
If the difference between the set point and ambient
temperatures is less than 6.0 °C then the set point can be
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Our performance data was produced at the ideal ambient
condition of 25.0 °C. For best performance the ambient
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Maximum ambient humidity is 70 % rH, non-condensing.
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exceeded.
A poorly located incubator can experience temperature
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cultures. These guidelines will ensure best performance:
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xIt should be in a well ventilated and dry area, but not
sited directly in the path of ventilation ducts.
xIt should be free from vibration.
xIt should be kept away from heat sources e.g. radiators,
heating pipes, refrigeration plant.
xIt should not be in direct sunlight.
xTo avoid contamination it should not be placed directly
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xThere should not be big swings in ambient temperature.
Leave a clearance of 50 mm from each side and 100 m from
the rear.
Level the incubator by adjusting the two front feet. Place a
small spirit level on the centre position of the middle shelf as
a guide.

Instructions for use
6CO2CELL Comfort 190_np_en 1301_mmm_V1.02
When it is level, secure the feet by tightening the lock nuts
with a 10 mm spanner.
4.2 SHELVES AND USABLE SPACE
Each incubator is supplied with 4 perforated stainless steel
shelves. The shelves locate into removable stainless steel
racking. The shelves incorporate an anti-tilt mechanism,
stopping the shelves from being pulled out too far.
Each shelf measures 690 mm wide by 390 mm deep. The
minimum spacing between shelves is 80 mm. There are
8 possible positions for the shelves.
The shelves and racking can be left in place during sterilisation.
The usable space within the incubator is the volume
in which the conditions are within our published
data.
A, B, C = Internal dimensions
a = distance from left and right wall = 70 mm
b = distance from top = 70 mm
c = distance from front and back wall = 70 mm
d = distance from base = 70 mm
For practical purposes this is the perforated area on each
shelf.
xDo not place any samples outside of this space.
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xDo not use the top shelf for very deep samples.
4.3 WATER TRAY
A stainless steel water tray is supplied which allows the
incubator to provide 90 %rH.
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the side racking.
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water.
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week.
Any spillages within the chamber should be removed and
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cause condensation to form.
Under no circumstances should any equipment be placed in
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Remove the water tray before moving the incubator.
Empty the water tray if the incubator is switched off,
otherwise condensation will occur.
4.4 GAS CONNECTION
Risk of injury. Compressed gas.
Risk of damage to unit.
Observe relevant regulations for
handling CO2
Risk of asphyxiation. Site must have
adequate ventilation.
Risk of CO2poisoning. Site must have
adequate ventilation.
The incubator is designed for use with CO2of
99.5 % purity. Input pressure greater than 1.0 bar
(15 psi) will damage the incubator so a pressure
reducing valve (PRV) must be used.
The CO2inlet to the incubator is via a 4 mm barbed push
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can be supplied by MMM. DSection 10.
For correct connection of CO2, follow these steps in order:
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supply.
2) Fully close the PRV.
3) Open the main valve on the gas bottle or central supply.
4) Adjust the PRV pressure of the gas supply to between
0.3 bar and 0.7 bar (4.5 to 10 psi).
5) Close the main valve on the gas bottle / central supply.
6) Connect the other end of the tubing to the incubator CO2
inlet. Secure with the supplied clip.
Repeat these steps whenever you change the CO2bottle or
supply.
Turn off the main valve and disconnect from the CO2supply
if the incubator is to be unused for extended periods.
4.5 ELECTRICAL CONNECTION
Risk of injury.
Risk of damage to unit. Observe correct
mains supply voltage.
Risk of electrical shock. Do not connect
with wet hands.
No user serviceable parts. Do not
remove service panels.
Disconnect unit in an emergency.
Before connecting the incubator, check that the electrical
supply corresponds with the values on the incubator data
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The incubator is designed to operate from a single phase
electrical supply of: 230 V AC ±10 % 50/60 Hz.
This incubator MUST be connected to a protective earthed
supply.
Further details can be found in the technical data.
DSection 15.1.

MAIN DISPLAY
The main screen shows chamber set points (SP) and
process values (PV). It also shows the status of any
alarm and has touch screen buttons for accessing further
functions.
5.4 CHANGING TEMPERATURE SET
POINTS AND HIGH / LOW ALARMS
To change the temperature set point, touch this area of the
main screen:
The high and low alarm limits are relative to the chamber set
point.
For instance if the set point is 37.0 °C and the high alarm
limit is + 1.0 °C then the alarm will trigger at 38.0 °C.
If the set point is changed to 36.5 °C then the high alarm will
trigger at 37.5 °C.
Instructions for use
CO2CELL Comfort 190_np_en 1301_mmm_V1.02 7
Plug the supplied power lead into the IEC connector on the
rear of the incubator.
Plug the other end of the IEC lead into the electrical supply.
In an emergency you may need to isolate
the incubator from the electrical supply by
disconnecting the mains supply plug. Make sure
that the mains supply plug is easily accessible.
5 GETTING STARTED
5.1 START UP
Insert the pre-formatted SD card (supplied) into the slot to
the right of the controller.
Turn on the unit using the power switch on the rear panel.
The controller will boot up and perform a self-check.
5.2 SETTING TIME AND DATE
After the controller has booted up, the time and date screen
will be shown. Time and date are shown in the format
hh:mm:ss and dd/mm/yy.
If the time and date are incorrect press the time display. This
screen will be shown:
5.3 FACTORY PRESETS
The following values are preset to allow you to work straight
away:
Parameter Value
Set Point, Temperature 37.0 °C
Set Point, CO25.0 %
High temperature alarm +1.0 °C
relative to set point
Low temperature alarm -1.0 °C
relative to set point
High CO2alarm +1.0 °C
relative to set point
Low CO2alarm -1.0 °C
relative to set point
Door open alarm timer 30 seconds
Touch here to alter set point
Touch here to alter high alarm limit
Touch here to alter low alarm limit
If the time and date is correct
press:
Proceed to step 5.3
Use the touch screen to enter
the correct time.
Press WRFRQ¿UP
selection.
Repeat these steps for setting
the date.
Temperatureprocess value Chamber temperature set point
CO2process value Chamber CO2set point
Sterilise cycle start Menu escape
Touch here to alter set point
Touch here to alter high
alarm limit
Touch here to alter low
alarm limit
Use the keypad to
change the set point.
Maximum set point is
limited to 60.0 °C
Minimum set point is
limited to 25 °C
Press WRFRQ¿UP
selection.
Use the keypad to
change the high alarm
threshold.
Press WRFRQ¿UP
selection.

Instructions for use
8 CO2CELL Comfort 190_np_en 1301_mmm_V1.02
5.5 CHANGING CO2SET POINTS AND
ALARM THRESHOLDS
To change the temperature set point, touch this area of the
main screen:
Use the keypad to
change the set point.
Maximum set point is
limited to 20.0 %
Minimum set point is
limited to 0.0 %
Press
to
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If the CO2set point is 0.0 % then CO2control is
disabled.
Touch here to alter set
point
Touch here to alter
high alarm limit
Touch here to alter low
alarm limit
5.6 ALARM STATUS SCREEN
The alarm icon gives a visual indication of alarm
status.
Pressing the alarm icon opens the alarm status screen:
High temperature alarm Low CO2alarm
Low temperature alarm Door open alarm
High CO2alarm SD card alarm
Low gas pressure alarm
In an alarm condition an audible buzzer sounds and the
alarm icon changes to red.
Press this red alarm icon and the alarm status screen will
show the corresponding alarm will in red.
For instance this shows high temperature alarm condition:
Pressing this will mute the alarm.
The alarm is now acknowledged and the icons show this by
changing to yellow:
The icons remain yellow for as long as the incubator is in an
alarm condition.
This process is the same for all alarms.
Use the keypad to
change the low alarm
threshold.
This must be negative
VRUHPHPEHUWR¿UVW
press
Press WRFRQ¿UP
selection.
Use the keypad to
change the low alarm
threshold.
This must be negative
VRUHPHPEHUWR¿UVW
press
Press WRFRQ¿UP
selection.
Use the keypad to
change the high alarm
threshold.
Press WRFRQ¿UP
selection.

Instructions for use
CO2CELL Comfort 190_np_en 1301_mmm_V1.02 9
As an additional warning the colours of the process values
change during alarm condition.
The temperature process
value has changed
colour from black to red.
The CO2process value
will change colour from
blue to red when it
enters alarm conditions.
6 OTHER FEATURES
6.1 GRAPHICAL DISPLAY
The controller has a built in feature that allows the user
to view graphs of both temperature and CO2. It stores a
minimum of 3 days of graphical data. When the internal
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Graphs are only stored while the unit is powered.
This feature is independent of the SD data logging function.
DSection 7.
On the main screen press the graph symbol:
In this instance we are viewing the temperature graph:
Press the
y-axis limits.
The values can be
changed using the
keypad.
Press
Use to view the temperature
stability against time.
To return to the Main Screen press the escape button
The CO2graph is viewed in the same manner.
6.2 UTILITY MENU
On the main screen press the utility menu symbol:
The utility menu combines all of the incubator settings
menus in one place. You will already be familiar with some
of these.
Clock menu. DSection 5.2
Temperature menu. DSection 5.5
CO2menu. DSection 5.6
User security menu. DSection 6.6
SD card menu. DSection 7
Door alarm menu. DSection 6.3
Alarm status menu. DSection 5.7
Historical event log. DSection 6.5
Incubator information. DSection 6.4
User log out. DSection 6.6
Ö
The graph menu
opens:
Choose which graph
you wish to view

Instructions for use
10 CO2CELL Comfort 190_np_en 1301_mmm_V1.02
6.3 DOOR ALARM MENU
Press the door icon:
The door has a
magnetic switch that
senses when the door
is opened.
The door icon turns red as soon as the door is opened. After
a set time the alarm will sound. This can be muted in the
alarm status menu. DSection 5.7
This delay can be changed by pressing here.
The format is mm:ss.
6.4 INCUBATOR INFORMATION
Press the info icon:
The information screen shows the incubator Serial Number
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is using. This information is important should you require
any assistance from MMM.
6.5 HISTORICAL EVENT LOG
The controller has a built in feature that displays the time
and date of 100 historical events.
When 100 events have been logged the oldest event is
over-written.
Events include: alarms, alarm acknowledgment, door open/
closed, power on/off, SD card insertion/removal,
XVHUORJRQORJRIIVWHULOLVHF\FOHVWDUW¿QLVKDERUW
Press the event log icon:
Press to scroll
though the events.
6.6 USER SECURITY
This incubator allows for a user name and password
to be programmed.
This stops any unauthorised changes to incubator settings.
6.6.1 ENTERING AUSER NAME AND
PASSWORD
To enter a user name and password press the User icon:
The user name and password are now saved.
6.6.2 ENABLING SECURITY
Press the security status icon:
The icon changes state to:
Security feature is enabled and you are now logged
on as shown by the open padlock symbol:
Press here on Line 1 to
enter user name.
Press: to
save.
Then press here to
enter password.
Press:
to save

Instructions for use
CO2CELL Comfort 190_np_en 1301_mmm_V1.02 11
6.6.3 LOGGING OUT
On the utility menu screen, press and hold the log out icon:
You are logged out and the padlock icon is now locked:
No changes to the incubator can be made without logging
back in.
For the Comfort 190S incubator only:
At this point the magnetic door locks are activated, stopping
any unauthorised access to the incubator.
6.6.4 DISABLING SECURITY
Press the utility icon:
When prompted, enter the user name and password.
To disable security press:
Icon changes to:
Security is now disabled.
7 SD CARD DATA
LOGGING
The incubator is supplied with a 2 GB SD card. This
SD card is pre-formatted to work with the
controller’s operating system.
Only cards supplied by MMM can be used. Using
any other type of card will not work with the
incubator and will cause the controller system to
crash.
The controller can log to a removable SD card located on
the front of the incubator.
Every 30 seconds the controller records the following data
to the SD card:
Date & Time
Chamber temperature set point (SP);
Chamber temperature process value (PV)
Door set point (SP);
Door process value (PV)
Door set point (SP);
Door process value (PV)
CO2set point (SP);
CO2process value (PV)
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The data is stored in Excel format making it very easy to
read and use the data with a PC.
Data is only logged when the incubator is powered; however
data is not lost when power is removed.
7.1 START LOGGING
If the card is already inserted when the incubator is powered
up then logging will start automatically.
If the SD symbol is grey then the incubator is
logging. If it is red then it is not logging.
Press the red SD icon in the Utility Menu. The Status
screen will state that the card is removed.
Insert the SD card into the slot to the right of the controller
(label side closest to controller).
The SD Card status screen shows that the controller is
locating the data and preparing to log. This can take from a
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Please be patient.
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shows that it is logging data:
Press to return
to the main screen

7.3 REMOVING THE SD CARD
Always perform the following steps before
removing the SD Card. Failure to follow
these steps will results in corrupted data.
On the SD Card status screen press the SD card tools icon:
It is now safe to
remove the SD card.
When the card is removed then the screen will automatically
change to this:
Instructions for use
12 CO2CELL Comfort 190_np_en 1301_mmm_V1.02
7.2 CHANGING FILENAME
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3UHVVWKH¿OHQDPHRQWKHVFUHHQLQWKLVFDVH12345. Enter
DQHZ¿OHQDPHLQWKLVFDVH
Press
to store selection.
Press WRFRQ¿UPWKHQHZ¿OHQDPH
7KHFRQWUROOHUZLOOWKHQORFDWHWKHQHZ¿OHDQGVWDUWORJJLQJ
Press the remove card
icon:
3UHVVWKHFRQ¿UP
icon:

This shows that the door locks have activated and that
Operator 6 is logged on.
Press to
proceed.
8.3 STERILISE CYCLE
The sterilise cycle has now started.
A temperature graph is plotted to show
the duration of the cycle. The y-axis
automatically changes its scale to 205 °C
This shows that
the heaters are
operational
Abort cycle icon
DSection 8.5
The sterilise cycle consists of 3 distinct phases. They are:
xHeat up.
During the heat up phase, maximum power is applied to
the heating elements. CO2control is disabled to protect the
sensor and the door locks are activated.
x2 hour dwell.
The dwell phase starts once the chamber temperature
reaches 200 °C.
Heater power is automatically reduced to regulate the
temperature at 200 °C.
xCool down.
After 2 hours at 200 °C power is removed from the heaters
and the cooling phase begins. The chamber is allowed to
cool naturally. When the chamber temperature reaches
38 °C the door locks are deactivated and CO2control is
enabled.
The time taken for a full sterilisation cycle is
dependent on the cool down phase.
As cooling relies on the heat loss through the chamber walls
the time taken is affected by the location of the incubator
and the ambient temperature.
So, an incubator placed under a worktop will take longer to
cool than a freestanding incubator on a worktop.
We recommend that you carry out the sterilise cycle at the
end of a working day. That way, when you return to work in
the morning the incubator will be ready for you to use.
Instructions for use
CO2CELL Comfort 190_np_en 1301_mmm_V1.02 13
8 HIGH TEMPERATURE
STERILISATION
Risk of damage to unit.
The Comfort 190 is not intended to be used as
an autoclave and must not be used for sterilising
any other equipment.
Only the shelves, racking and water tray can be
left inside the incubator during sterilisation.
Risk of injury. Risk of burning. Internal surfaces
reach 200 °C
The Comfort 190S incubator is equipped with a high
temperature sterilisation function. This raises the chamber
temperature to 200 °C for 2 hours.
Independent evaluation has shown this to be effective
against all spores and bacteria commonly associated with
laboratory incubator contamination.
8.1 PREPARATION
Make sure that you have entered a user name and
password as shown in DSection 6.6.1
Empty and dry the water tray. This can be placed back
inside the incubator.
Clear any samples or equipment from the shelves.
The shelves and racking can be left in place during the
sterilisation cycle.
8.2 STARTING THE CYCLE
Press and hold the sterilise button for 5 seconds:
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Automatically carried
out by controller.
When you have emptied the water tray, place it back inside
the chamber and close the doors.
Press the grey tick to acknowledge this:
All checks are now
complete.
Press to
proceed.

Instructions for use
14 CO2CELL Comfort 190_np_en 1301_mmm_V1.02
7KLVVWHULOLVHF\FOHJUDSKVKRZVDW\SLFDOVWDUWWR¿QLVK
duration of 8 hours:
8.4 COOLING PHASE
After the 2 hour dwell is completed the incubator goes into
the cooling phase of the sterilisation cycle.
The doors are locked during this phase. CO2control is
disabled.
The chamber
temperature is
displayed
The cooling phase ends once the temperature falls below
38.0 °C
Press
to continue
to main screen.
8.5 ABORTING THE STERILISE CYCLE
Should you need to stop the sterilise cycle for any reason
then press the abort icon:
Press The incubator now enters the cooling phase.
DSection 8.4.
9 ERROR MESSAGE
SCREENS
In the event of a fault condition the control system may warn
you with various error messages.
Chamber probe failure
Chamber probe is out of
limits and has failed.
Contact MMM Service
immediately
Base probe failure
Base probe is out of limits
and has failed.
Contact MMM Service
immediately
Door probe failure
Door probe is out of limits
and has failed.
Contact MMM Service
immediately

SD card critical error
SD has not been safely
removed.
Press and hold
Switch
power off, insert card and
switch power on again.
If problem persists contact
MMM Service
SD card removal error
Repeat the steps above to
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Boot screen error
Take a picture of the
screen. Turn power off for
1 minute.
If problem persists contact
MMM Service with details
of picture.
10 OPTIONS
10.1 VOLT FREE ALARM RELAY (BMS)
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volt free changeover relay for connection to a building
maintenance system (BMS).
This relay changes state whenever the incubator is in an
alarm condition. The relay changes back to its original state
once the alarm condition clears.
The connections are as shown below:
10.2 4 - 20 mARETRANSMISSION (RETX)
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ordering.
This feature allows the chamber temperature and CO2
process values to be retransmitted as a 4 - 20 mA signal.
This 4 - 20 mA signal can be connected to external
equipment for independent validation of incubator
performance.
Instructions for use
CO2CELL Comfort 190_np_en 1301_mmm_V1.02 15
The incubator will be supplied with a pre-wired 3 metre
cable socket.
The temperature signal is scaled as: 4 mA = 0 °C;
20 mA = 250 °C
The CO2 signal is scaled as: 0 mA = 0 % CO2;
20 mA = 20 % CO2
10.3 RS 232 SERIAL COMMUNICATION
(232)
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the SD card data to be remotely accessed and downloaded.
A PC with a serial (COM) port is required. Software is
required. Please contact MMM limited for further details.
10.4 ETHERNET COMMUNICATION (ETH)
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ordering.
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network. This allows the SD card to be remotely accessed
and for the incubator screen to be displayed on a PC.
Connection to a PC network is required. Contact your IT
manager to see if this possible.
Instructions will be sent separately when this option is
ordered.
10.5 MULTI-DOOR (8ID)
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The inner glass door can be replaced with a multi-door. This
has 8 smaller glass doors in a stainless steel frame.
By only opening one small door to access the sample you
require, less heat, humidity and CO2are lost.
This improves recovery of the chamber back to ideal
conditions and reduces the amount of CO2used.
11 ACCESSORIES
This is the list of some of the accessories currently
available.
Please contact MMM if the part you require is not listed.
PTSK Stacking kit for 2 x Comfort 190
PTST1 Wheeled platform for Comfort 190
PRV Single stage CO2pressure reducing valve
PRV2 Two stage CO2pressure reducing valve

Instructions for use
16 CO2CELL Comfort 190_np_en 1301_mmm_V1.02
RO6 In line CO2reducing valve with gauge
(0.1 to 2 bar)
PNEU Automatic change over unit for two CO2
cylinders
TYGON Tygon gas tubing, per meter
12 CLEANING
Electrical hazard. Do not spill water or
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surfaces.
Disconnect before cleaning.
Completely dry the unit before
reconnecting the power.
12.1 OUTER SURFACES
Always disconnect the incubator before cleaning.
It is recommended that the exterior of the incubator regularly
wiped clean. Use a non-abrasive soft cloth dampened with a
warm soapy water solution.
Make sure the exterior is thoroughly dry after cleaning.
12.2 INNER SURFACES
It is advisable to clean the chamber regularly.
Use a 70 % isopropanol 30 % distilled water solution.
Apply using a sterile cloth so as not to introduce any
contamination.
Never use any of the following:
xAny chlorine based detergents or bleaches.
xAny acidic cleaning agents.
xSodium Azide
xIodine
xFerric Chloride
Wipe over the inner surfaces of the stainless chamber, all
the shelves including the underside of the shelves, the shelf
racks and the water tray.
The shelves, racking and water tray can be sterilised in an
autoclave if required.
Clean the glass door and the inner gasket seal.
Clean the inner stainless steel surfaces of the outer door
and the outer door gasket.
12.3 OXIDATION AT HIGH TEMPERATURE
(COMFORT 190SONLY)
When operated at 200 °C the action of atmospheric oxygen
may cause discolouration of the stainless steel surfaces.
This natural oxidation is not detrimental to the quality of the
incubator and is completely harmless.
13 SERVICE
13.1 WARRANTY
All MMM Comfort 190 incubators are supplied with a two
year manufacturer’s warranty from the date of installation.
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Registration Card and post to MMM Limited or to your local
distributor.
13.2 PREVENTATIVE MAINTENANCE
This Culture Safe CO2Incubator is built to last for many
years, but to ensure that the unit continues to perform
to the highest level we recommend that you purchase a
preventative maintenance contract from MMM.
The incubator should be calibrated annually.
13.3 RETURNING AUNIT
Should your incubator require repair or maintenance then
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Returns to MMM will only be accepted with prior
authorisation from the Service Department.
We recommend that you keep all packaging.
When returning a unit please include the following
information:
xIncubator model and serial number
xDate of purchase
xAuthorisation number
xName of local distributor if not purchased from MMM
xA detailed description of the fault (we can never have too
much information)
xWhere was the incubator located
xContact details of the person who reported the fault
x $FRPSOHWHGGHFRQWDPLQDWLRQFHUWL¿FDWHSOXVDFRS\
faxed through in advance)
MMM cannot accept any returns without a completed
GHFRQWDPLQDWLRQFHUWL¿FDWH

Instructions for use
CO2CELL Comfort 190_np_en 1301_mmm_V1.02 17
14 DISPOSAL
The incubator must not be disposed of at public waste
collection points. It must only be disposed of by licensed
waste recycling companies.
Contact MMM for further information regarding WEEE
regulations.
15 TECHNICAL
INFORMATION
15.1 TECHNICAL DATA
TEMPERATURE MANAGEMENT
Range °C 5 °C above ambient to
60 °C
Control °C 0.1 °C
Stability °C ±0.1 °C @ 37.0 °C
Uniformity °C ±0.25 °C @ 37.0 °C
Door recovery,30 s
opening
minutes 13
CO2MANAGEMENT
Range % CO20-20 %
Control % CO2±0.1 %
Stability % CO2±0.2 %
Uniformity % CO2±0.2 %
Door recovery, 30 s
opening
minutes 6
CO2sensor type NDIR
CO2inlet pressure Bar 0.3 to 0.7
CO2inlet connection type PPSXVK¿WEDUEHGW\SH
HUMIDITY MANAGEMENT
Humidity control type Passive
Range % rH 90 % ± 5 %
Door recovery, 30 s
opening
minutes 50
ELECTRICAL DATA
Nominal voltage 50/60 Hz V 230 1N ~PE
Power; Comfort 190 W 380
Power ; Comfort 190S
sterilise cycle
W 1800
Power consumption kWh 0.058
Main fuse F1; Comfort 190 6.3 A / 6.3 x 32mm / 250 V / TT
Main fuse F1;
Comfort 190S
10 A / 6.3 x 32mm / 250 V / TT
EXTERIOR DIMENSIONS
Width mm (inches) 765 (30.1)
Height mm (inches) 862 (34)
Depth mm (inches) 734 (28.9)
Wall clearance, rear mm (inches) 100 (3.94)
Wall clearance, side mm (inches) 50 (1.97)
INTERIOR DIMENSIONS
Width mm (inches) 632 (24.9)
Height mm (inches) 686 (27)
Depth mm (inches) 440 (17.3)
Volume Litres (cu. ft.) 190 (6.7)
Number of shelves 4 shelves. Maximum of 8
SHIPPING DIMENSIONS
Width mm (inches) 925 (36.4)
Height mm (inches) 1080 (42.5)
Depth mm (inches) 850 (33.5)
WEIGHT
Comfort 190, Net kg (lbs) 94.5 (208)
Comfort 190S, Net kg (lbs) 104 (229)
Comfort 190, Shipping kg (lbs) 109 (240)
Comfort 190S, Shipping kg (lbs) 117.5 (259)
15.2 DIMENSIONAL DRAWINGS

Instructions for use
18 CO2CELL Comfort 190_np_en 1301_mmm_V1.02