MultiChannel uPA16 User manual

Micro Preamplifier Manual
μPA16
μPA32

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© 2016 Multi Channel Systems MCS GmbH. All rights reserved.
Printed: 27. 07. 2016
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Table of Contents
1Important Information and Instruction 1
1.1 Operator's Obligations 1
1.2 Guarantee and Liability 1
1.3 Important Safety Advice 2
2Welcome to the μPA 3
3Setting Up and Connecting the μPA 4
3.1 General Setup Recommendations 4
3.2 Multi Channel Experimenter  6
3.3 Setting up MC_Rack 9
3.3.1 Noise Level Test 9
3.3.2 ME/W-SG Test 10
3.4 Service and Maintenance 11
3.4.1 Cleaning the Connectors 11
3.4.2 Sterilization 11
4μPA16 12
4.1 Pin Layout μPA16 13
4.1.1 Input Connector 13
4.1.2 Output Connector 14
4.2 Technical Specifications μPA16 15
5μPA32 16
5.1 Pin Layout μPA32 17
5.1.1 Input Connector 17
5.1.2 Output Connector 18
5.2 Technical Specifications μPA32 19
6Contact Information 20

Important Information and Instruction
1
1 Important Information and Instruction
1.1 Operator's Obligations
The operator is obliged to allow only persons to work on the device, who
 are familiar with the safety at work and accident prevention regulations and have been instructed
how to use the device;
 are professionally qualified or have specialist knowledge and training and have received instruction
in the use of the device;
 have read and understood the chapter on safety and the warning instructions in this manual and
confirmed this with their signature.
It must be monitored at regular intervals that the operating personnel are working safely.
Personnel still undergoing training may only work on the device under the supervision
of an experienced person.
1.2 Guarantee and Liability
The General conditions of sale and delivery of Multi Channel Systems MCS GmbH always apply.
The operator will receive these no later than on conclusion of the contract.
Multi Channel Systems MCS GmbH makes no Guarantee as to the accuracy of any and all tests and
data generated by the use of the device or the software. It is up to the user to use good laboratory
practice to establish the validity of his findings.
Guarantee and liability claims in the event of injury or material damage are excluded when they are
the result of one of the following.
 Improper use of the device.
 Improper installation, commissioning, operation or maintenance of the device.
 Operating the device when the safety and protective devices are defective and / or inoperable.
 Non-observance of the instructions in the manual with regard to transport, storage, installation,
commissioning, operation or maintenance of the device.
 Unauthorized structural alterations to the device.
 Unauthorized modifications to the system settings.
 Inadequate monitoring of device components subject to wear.
 Improperly executed and unauthorized repairs.
 Unauthorized opening of the device or its components.
 Catastrophic events due to the effect of foreign bodies or acts of God.

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1.3 Important Safety Advice
Warning: Make sure to read the following advices prior to install or to use the device and the software.
If you do not fulfill all requirements stated below, this may lead to malfunctions or breakage of
connected hardware, or even fatal injuries.
Warning: Obey always the rules of local regulations and laws. Only qualified personnel should be
allowed to perform laboratory work. Work according to good laboratory practice to obtain best
results and to minimize risks.
The product has been built to the state of the art and in accordance with recognized safety engineering
rules. The device may only
 be used for its intended purpose;
 be used when in a perfect condition.
 Improper use could lead to serious, even fatal injuries to the user or third parties and damage to the
device itself or other material damage.
Warning: The device and the software are not intended for medical uses and must not be used
on humans.
 Malfunctions which could impair safety should be rectified immediately.
 Any physical damage of the cable, such as a broken cable, causes a physical damage of the micro
preamplifier that cannot be repaired.
 Protect the device from heat. Do not autoclave!
 You can clean the connectors with distilled water in an ultrasonic bath, but keep the cable away
from the fluid. Dry the device with compressed air.
 Use and keep the device always in a dry environment. Do not expose it to fluids or vapor for a longer
period of time.

3
2 Welcome to the μPA
The μPA micro preamplifier with 16 or 32 electrode inputs is connected to the microelectrode probes
for providing the initial tenfold amplification stage. Gain might be different from default value
according to customer specification. The use of a headstage preamplifier unit optimizes the signal to
noise ratio.
The micro preamplifier is equipped with an Omnetics socket for NeuroNexus probes. It has an additional
common ground and a reference electrode input. The reference electrode is ideally identical to the
recording electrodes and placed into a comparable but inactive area or tissue. Background or noise
signals that are picked up by both the reference electrode and the recording electrodes are removed.
The metal case provides electrical shielding. The very high input impedance ensures stable long-term
recordings.
Ideally, the input impedance would be infinite to minimize electrical current. As low voltages are
generally recorded, a high current would flow if the input impedance were low. As a result, the
amplifier would not be able to deliver the current, and the voltage would break down.
The micro preamplifier has a high input impedance to avoid this problem. Adapters for all standard
microelectrodes such as NeuroNexus probes (from NeuroNexus ®) for acute and chronic
implantations ensure a close proximity to the probe to minimize signal loss and cross talk. Adapters are
not included in the standard scope of delivery and have to be ordered separately. For custom adapters,
please ask your local retailer or contact Multi Channel Systems MCS GmbH.
To start working with the μPA and to learn how to handle the system the ME/W Signal Generator
is a convenient tool. The ME/W Signal Generator can produce analogue signals identical to those from
a variety of biological samples and is therefore a convenient method to test the functionality of the
recording system. The ME/W-SG is not included in the standard scope of delivery and has to be ordered
separately. Please read the ME/W-SG data sheet for more information.

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3 Setting Up and Connecting the μPA
3.1 General Setup Recommendations
In the following, you find general recommendations for the installation.
Important: It is important that the complete setup refers to a single common ground. The reference
input has always to be connected. It is recommended to use a reference electrode. However, if you
are not using a reference electrode, connect the reference input to ground (GND). Otherwise, noise
picked up by the reference input will be subtracted from the recording signals. This will either lead to
signal loss or to a very high noise level.
1. Ground the animal with a ground electrode of large surface area, for example, a liquid gel adhesive
electrode, that is connected to the ground of the setup, for example, a large metal table or a Faraday
cage, to avoid pickup of noise from the environment. The ground electrode is best positioned in an
electrically inactive region not near muscle, nor heart, for example, at the belly.
2. Connect the GND input of the micro preamplifier to the common ground of the setup. The ground
input is internally connected to the metal case of the preamplifier.

5
3. Connect the reference electrode REF to the reference input of the micro preamplifier. Generally,
a reference electrode is inserted into non-active tissue of the experimental model. The reference
electrode should be identical to the recording electrode so that both electrodes see the same
background noise. This is necessary because despite the grounding, the animal’s body often has
not exactly a potential of zero, due to the electrode impedance, for example. The background noise
is then subtracted from the recording signal, increasing the signal to noise ratio. Please note that this
may not work if the complete setup is not properly grounded.
4. (Optional) If two reference electrodes are used, connect the second electrode to the free reference
input. Otherwise, leave it free. It is generally not necessary to use a second reference electrode. It can
be used to enlarge the surface area of the reference, though.
5. Connect all unused recording channels to the GND input or to the reference electrode, if ground and
reference are short circuited, to avoid picking up noise. As the total amplifier gain generally lies in the
range of 1000, even very small noise signals may generate high noise signals.
6. If you are not using a Faraday cage, it might be necessary to shield the complete setup with aluminum
foil or similar to prevent electrical interference from the outside. Connect the aluminum foil to the
ground of the setup, for example, the metal table.
Troubleshooting: If you observe problems with noise, check that the ground of the setup is connected
to exactly the same ground as the data acquisition computer. For example, connect the metal table
to the ground / earth wire of a free power outlet (of the same electrical system), as the computer is
generally connected to ground / earth via the power plug, too. Also, connect the data acquisition
computer’s metal case with a thick ground wire to the ground of the setup, for example the metal
table, preferably with a 4 mm plug.

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3.2 Multi Channel Experimenter 
Double-click the Multi Channel Experimenter icon or select Multi Channel Experimenter from the "Start"
menu. The program starts. The main window opens automatically: "Multi Channel Experimenter
(Instance: 1)". It is possible to open up to four instances in parallel. Operation is independent for each
instance.
This is your virtual rack configuration in the "Experiment Setup" window, which is blank after program
start. On the left side five classes of instruments are provided: "Data Sources", "Recorder" and
"Stimulators" are hardware components. The virtual instruments available up to now are divided in
"General" and application specific "Neuro".
The header of the dialog contains five buttons: "Start DAQ", "Start Recording", "Load Experiment" and
"Save Experiment" in the main toolbar on the left and the "Menu" button on the right. In the bar down
under the "Recording Time" and the "DAQ Time" are registrated.

7
Building a Rack
It is very easy to build a rack: Please click onto the button of the desired instrument and move it via drag
and drop from the left to the right side of the main window!
It is also easy to connect the instruments: Please click onto one of the symbols on the lower part of
an instrument button to connect this output port via drag and drop with the input port (symbol on
the upper side) of the target instrument. Please read the next chapter.

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Conventions for Building a Rack
Please consider the rules for connecting the instruments. The Multi Channel Experimenter program allows
only connections between output ports at the lower side of the device to the input port on the upper side
of the target device. The input and output ports of the devices are color coded for a better assignment of
the instruments. The blue symbol represents the port for the "Electrode Raw Data". The green symbol
represents the port for the "Auxiliary Analog Data". The red symbol represents the port for the "Digital
Data".
Three color coded output ports of the MEA2100 data acquisition device.
Example: Click on the blue symbol output port of the data source and connect it by drag and drop with
the blue symbol input port of the filter. The electrode raw data stream will flow from the data source to
the filter. Connect the blue symbol output port from the filter with the black symbol input port of the
recorder.
The electrode raw data stream will flow from the filter to the recorder. The black input port of the
recorder accepts all types of input data streams and you can connect more than one data stream.
The Multi Channel Experimenter Rack
Move as many instruments as you need via drag and drop into the “Experiment Setup” window.
It is possible to use the virtual tools more than one time.

9
3.3 Setting up MC_Rack
Please refer to the MC_Rack Manual for more information.
1. Start MC_Rack. Click Data Source Setup on the Edit menu. Select a 1-dimensional layout. Select the
number of electrode channels, 16 channels when using a μPA16, 32 channels when using a μPA32.
Deselect the check box for the digital channel if you do not need it.
2. Add the data acquisition to your virtual rack.
3. In the tree view pane of the virtual rack, select the data acquisition, and click the Hardware tab. Enter
the total amplifier gain according to the specifications of the instruments. For example, for a micro
preamplifier with a gain of 10 and a following filter amplifier with a gain of 100, the total gain is 1000.
4. On the Edit menu, click Add Data Display to add a raw data display to your virtual rack.
5. In the virtual rack tree view pane, select the Display 1 and click the Layout tabbed page. Set up a
channel map with channels 1 to 16 or 1 to 32 or select “Default Map”.
6. Adjust the display ranges to 1000 ms and +/–50 μV.
3.3.1 Noise Level Test
Typical results of a noise level test are shown in the following screen shots. The Portable ME32-System
used for this test had a total gain of 1000 and a bandwidth of 1 to 5000 Hz. If you use a filter amplifier
with a narrower bandwidth, you can generally expect a slightly lower noise level.
To test the noise level, please connect the μPA32 to a Portable ME32-System, for example, but without
electrode probe. The noise level is 60 μV peak to peak. The window shows electrode raw data.

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3.3.2 ME/W-SG Test
The following screen shots show the results of a test with a ME/W-SG signal generator. Please read
the data sheet for more information.
Connect the ME/W-SG to the μPA32 via Omnetics connector on the bottom of the signal generator.
The Omnetics connector of the μPA is lettered in black and the Omnetics connector of the ME/W-SG
respectively. Please connect the devices in this way that the letters are on one side, facing to the bottom
of the ME/W-SG as shown on the picture.
Switch the dip switch into the desired position and press the control button to start the device.
The example below shows heart signals (ECG ventricle). The Portable ME32-System used for this
test had a total gain of 1000 and a bandwidth of 1 to 5000 Hz.

11
3.4 Service and Maintenance
3.4.1 Cleaning the Connectors
Warning: It is recommended to avoid the use of cleaning solutions to avoid corrosion. If a wet
cleaning is required, use distilled water. Make sure that only the connectors touch the liquid; do not
submerge the micro preamplifier or the cable. Otherwise, you can fatally damage the electronics.
 Clean the connectors with 70 % alcohol and cotton swabs from time to time.
 If this does not provide satisfying results, insert only the connectors into a small beaker with acetone
or alcohol and treat them in an ultrasonic bath for 10 to 20 s. Air-dry the micro preamplifier for about
5 min before use.
 You can also clean the connectors with distilled water in an ultrasonic bath. Dry the connectors with
compressed air immediately after sonication and let the preamplifier air dry for at least 6 h before use.
3.4.2 Sterilization
Warning: Do not autoclave or sterilize micro preamplifiers by high heat (above 70 °C) or vapor.
The material is not heat-stable and may deform under heat. Vapor can lead to a corrosion of the
electronics.
Micro preamplifiers can be sterilized with standard methods that are not based on high heat or vapor,
for example, with 70 % alcohol, UV-light, or by thermal sterilization in an oven at 56 °C with an
incubation time of 8 hours.

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4 μPA16
The micro preamplifier μPA16 is equipped with an Omnetics socket. You can directly connect different
types of NeuroNexus probes (from NeuroNexus ®, www.neuronexustech.com) without the need of an
adapter. Please see below. Please find the connectors and corresponding mating connectors listed in
chapter “Technical Specifications”.
NeuroNexus Electrode Probes, for example:
 NeuroNexus AXIAL
 NeuroNexus C16
 NeuroNexus CM16
 NeuroNexus F16
 NeuroNexus H16
 NeuroNexus HP16
 NeuroNexus MRC16
 NeuroNexus MRCM16
To connect the following NeuroNexus probes with 32 electrodes you need two μPA16.
 NeuroNexus H32
 NeuroNexus HC32
Adapters for all standard microelectrodes such as NeuroNexus probes for acute and chronic
implantations ensure a close proximity to the probe to minimize signal loss and cross talk. Adapters are
not included in the standard scope of delivery and have to be ordered separately. For custom adapters,
please ask your local retailer or contact Multi Channel Systems MCS GmbH.

13
4.1 Pin Layout μPA16
4.1.1 Input Connector
two guide posts
This illustration shows the pin layout of the Omnetics connector A79039-001 (NSD-18-DD-GS
with 2 guide posts, female), viewed from the front, with the metal housing of the μPA16 above.
The reference input is used for connecting a reference electrode.
Pin Channel Pin Channel
Ground GND Reference REF
Pin 1 Channel 1 Pin 9 Channel 9
Pin 2 Channel 2 Pin 10 Channel 10
Pin 3 Channel 3 Pin 11 Channel 11
Pin 4 Channel 4 Pin 12 Channel 12
Pin 5 Channel 5 Pin 13 Channel 13
Pin 6 Channel 6 Pin 14 Channel 14
Pin 7 Channel 7 Pin 15 Channel 15
Pin 8 Channel 8 Pin 16 Channel 16

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4.1.2 Output Connector
The signal ground is connected to the ground of the amplifier. The signal ground
is used as the reference for the following filter amplifier.
Pin Channel Pin Channel
Pin 2 Channel 1 Pin 11 Channel 2
Pin 3 Channel 3 Pin 12 Channel 4
Pin 4 Channel 5 Pin 13 Channel 6
Pin 5 Channel 7 Pin 14 Channel 8
Pin 6 Channel 9 Pin 15 Channel 10
Pin 7 Channel 11 Pin 16 Channel 12
Pin 8 Channel 13 Pin 17 Channel 14
Pin 9 Channel 15 Pin 18 Channel 16
1, 10, 19, 23 Ground GND
20, 21, 22 Positive supply voltage
24, 25, 26 Negative supply voltage

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4.2 Technical Specifications μPA16
Operating temperature 0° C to 50° C
Storage temperature 0° C to 50° C
Relative humidity 10 % to 85 % non-condensing
Dimensions (W x D x H) ca. 17 mm x 17 mm x 2.5 mm w/o connector
ca. 21 mm x 17 mm x 2.5 mm with connector
Length of cable 1.5 m
Weight 1.5 g without cable
Maximum tensile strength
of cable
20 N
Input connector type 18-pin dual-row Omnetics NSD series A79039-001,
female
Mating connector type Omnetics NPD series
(NPD-18-xx-GS with 2 guide posts, male)
Output connector type 26-pin HD D-Sub, male (Harting TB 09 56 200 5615)
Number of amplifier channels 16
Supply voltage range ± 2.5 V
Supply current range < 15 mA, typically ± 14 mA
Gain 10
Bandwidth DC to 50 kHz
Input voltage range ± 250 mV (with respect to a supply voltage of 2.5 V)
Input impedance 1G@ 1 kHz
Input capacitance 13 pF
Input noise < 1.2 μVRMS (0.1 Hz to 10 Hz, inputs shortened)
Noise density en= 10 nV / Hz @ 1 kHz
Output voltage range ± 2.5 V (supply voltage range)

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5 μPA32
The micro preamplifier μPA32 is equipped with an Omnetics socket. You can directly connect different
types of NeuroNexus probes (from NeuroNexus ®, www.neuronexustech.com) without the need of an
adapter. Please see the list below. Please find the connectors and corresponding mating connectors
listed in chapter “Technical Specifications”.
NeuroNexus Electrode Probes, for example:
 NeuroNexus CM32
 NeuroNexus F32
 NeuroNexus H32
 NeuroNexus MRCM32
 NeuroNexus HP32
To connect the following NeuroNexus probes with 64 electrodes you need two μPA32.
 NeuroNexus H64
 NeuroNexus HC64
Adapters for all standard microelectrodes such as NeuroNexus probes for acute and chronic
implantations ensure a close proximity to the probe to minimize signal loss and cross talk. Adapters are
not included in the standard scope of delivery and have to be ordered separately. For custom adapters,
please ask your local retailer or contact Multi Channel Systems MCS GmbH.

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5.1 Pin Layout μPA32
5.1.1 Input Connector
four guide posts
This illustration shows the pin layout of the Omnetics connector A79023-001 (NSD-36-DD-GS
with 4 guide posts, female), viewed from the front, with the metal housing of the μPA32 above.
The reference input is used for connecting a reference electrode.
Pin Channel Pin Channel
Pin 1 Ground GND Pin 19 Channel 17
Pin 2 Reference REF Pin 20 Channel 18
Pin 3 Channel 1 Pin 21 Channel 19
Pin 4 Channel 2 Pin 22 Channel 20
Pin 5 Channel 3 Pin 23 Channel 21
Pin 6 Channel 4 Pin 24 Channel 22
Pin 7 Channel 5 Pin 25 Channel 23
Pin 8 Channel 6 Pin 26 Channel 24
Pin 9 Channel 7 Pin 27 Channel 25
Pin 10 Channel 8 Pin 28 Channel 26
Pin 11 Channel 9 Pin 29 Channel 27
Pin 12 Channel 10 Pin 30 Channel 28
Pin 13 Channel 11 Pin 31 Channel 29
Pin 14 Channel 12 Pin 32 Channel 30
Pin 15 Channel 13 Pin 33 Channel 31
Pin 16 Channel 14 Pin 34 Channel 32
Pin 17 Channel 15 Pin 35 Ground GND
Pin 18 Channel 16 Pin 36 Ground GND