N-BIOTEK NB-203QS User manual

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NB-203QS
Operation Manual
CO2 Incubator + 1 Shaker
Model: NB-203QS

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Table of Contents
NO.
CONTENTS
PAGE
1
SAFETY AND GENERAL PRECAUTION
3
2
TRANSPORTATION, STORAGE AND LOCATION OF INSTALLATION
5
3
PREREQUISIT AND CONFIGURATION
7
4
FEATURES AND SPECIFICATIONS OF THE UNIT
9
5
Control Panel
10
6
OPERATION
11
7
Safety Switch
16
8
Shaker Operation
16
9
How to detach the Shaker
18
10
Troubleshooting
19

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1. Safety and General Precaution
1. 1 General Information on Precaution
This equipment must be operated as described in this manual. if operational guideline is not
followed, equipment damage and personal injury can occur.
Please follow this mark to prevent equipment damage of personal injury.
If you don't keep this caution, you can get injured as well as a property loss.
1.1.1 Safety warning symbols
1.2 Precaution for using the power cable
Do not make the power plug be pressed by back of the product.
(A space between the product and the plug must be 30cm at least.
The power outlet must be only for this product.
(Using various products simultaneously can cause a fire)
Clean the power plug with a dry towel and connect it properly.
(Foreign substances or unsafe connection can cause a fire.)
Do not bend the power cable hardly and do not make it to be pressed by
heavy products. (When it is damaged, it can cause a fire.)
Do not touch the power code with wet hands. (It can cause an electric shock.)
Do not use the damaged power code and outlet.
(It can cause an electric shock and a fire.
When you see smoke coming from the product or smell burning,
you have to pull out the power code and stop using it.
(It can cause an electric shock and a fire.)

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1.3 Precaution for ground connection
Please ground before use the product, if you don't ground, you could get an electric shock
when malfunction or an electric leakage occurs.
At the place where you can't ground,
* Please buy the equipment to prevent any electrical leakage.
* An electric shock, electric leakage and firecan be occurred without electric leakage breaker.
Do not ground to these places; Gas Pipe, water pipe, pipe, lighting rod, telephone wire etc.
*Wrong ground connection can cause electrical leakage which eventually results in fire
If you don't have the outlet for AC 220V, then, bury it under the ground
after connecting the ground line to copper plate.
* No ground connection can bring an electrocution, an electric leakage and a Fire.
1.4 Precaution for use
You must not disassemble, fix and remodel the product by yourself.
(You can damage the product throughout a fire and malfunction
or get a property loss as well as experimental loss.)
Do not use the product for different purpose.
(It can cause malfunction or poor function. Consequently, you will get a wrong result.)
Do not use an inflammable spray near the product.
(The switch and other electric connection parts can cause a fire.)
When you use inflammable substances such as benzene, thinner, alcohol and LP gas.
Please be careful. (It can cause a fire and an explosion.)
To prevent water and experiment material from going into the control panel
during the experiment, make sure to clean the control panel with a dry cloth.
(It can cause an electric leakage and a fire.)
Do not wash the product with excessive quantity of water, thinner, benzene and Petroleum.
(It can cause an electric leakage, and malfunction or damage on the surface.)
When you don't use the product or clean it, please pull out the power plug.
(It is to prevent an eclectic leakage.)

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Open and close the door softly and please use a door knob.
(A heavy shock can damage the product and breakdown the operating part.
Also, your hands can be stuck between the door and body.)
Do not detach the built-in lamp and electrical devices.
(It can cause an electric shock and a fire.)
Please be sure to prevent foreign substances from getting into the sealing silicon of the door.
(The inflow of open air can cause the change of temperature in chamber and discoloration of
the packing part by a foreign substance.)
2. TRANSPORTATION, STORAGE AND LOCATION OF INSTALLATION
2.1 Transportation
DO NOT try to slide or tilt the unit
Lift the unit on each corner of bottom with the aid of 2 people.
Permissible ambient temperature range for transport: -10℃to 60℃.
2.2 Storage
Do not keep it at place in High Humidity. Permissible ambient humidity: max. 70%
storage in a cold location is the place you transfer the unit to the installation site for start-up,
condensation may form. In this case, Wait at least one hour until the CO2 incubator has
attained temperature and is completely dry.
Please check the voltage & Hertz written on serial label.
(Over-voltage, under-voltage can damage the product and poor performance.)
Do not install at a humid place.
(It causes an electric leakage accident and a corrosive of the product.)
Keep this product out of the direct ray of sun and do not install at a hot place or
a place that is near an electric heat.
(The proper indoor temperature is 20℃~ 30℃.)

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2.3 Location of installation and ambient conditions
Do not put flammable substances near the product. (It may cause a fire)
When you install the product, you are required to keep the distance at least 30cm
from the wall. To completely separate the unit from the power supply, power plug
must be disconnected. Install the unit in the way that the power plug is easily accessible
and can be easily pulled in case of danger.
Install the unit at a flat surface, free from vibration and in a well-ventilated location.
(If the ground is not flat, it may cause an excessive vibration of the product.)
When you move the product, do not lay down to its side or reverse the head
to bottom. (It may cause a malfunction.)
When you move the product, hold the door and other movable parts of
the product with a tape. (When the product is moved, the movable door can cause
injury of you and damage of the product.)
When you move the product, you must hold up the product.
(Pushing or pulling the product can damage the bottom part of the product.)

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3. PREREQUISIT AND CONFIGURATION
3.1 PREREQUISIT
Inspection of Boxes
When you have received the instrument, which is packed on pallet, inspectthe box carefully for any damages
that may have caused any damages to product during shipping.
Please report any damage to the carrier or to your local NBIOTEK distributor immediately.
LOCATION
The incubator is designed to operate at temperature 5℃above ambient, and recommended to operate
at minimum ambient (temperature in the place for use), 15℃. Maximum Room Temperature is 32℃.
To avoid place for use this incubator is as below.
1. Near Heater or Freezer (if it may generate heat and affect temperature control of incubator)
2. Near Equipment generating heat or cold air to incubator.
3. Directly Sunlight Exposed to incubator
4. Uneven ground or table head
5. The place where is being vibrated
Cleaning before use
Before conducting cell culture, it is recommended to clean up entire chamber and shelves,water trayby using
at least 70% Ethanol mixed of 30% distilled water and soft clothes.
Inserting shelves
Shelves are mounted onto the shelf racks in such a way that the edge of shelves which is slightly bent up
about 8mm goes to the back of the chamber until it is too close to the back wall. Basically, 3 shelve are
provided. Insert the shelves from top to bottom.
Level the incubator by adjusting feet. Place a small level on the second shelf of the incubator adjust the
levelling fee until the incubator is level and stable.
After inserting the shelves, place humidity tray in the bottom of chamber if humidification is required for your
application.

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3.2 CONFIGURATION.
Exterior Configuration of CO2 incubator.
<Figure #1>
Control panel
Air jacket
-6 side direct heating
Power switch
Ventilation fan
Glass door heater
-Prevent condensation Magnetic door seal
Stainless steel shelve Glass door
- Perforated to
improve uniformity
Inner door seal Door latch
- Airtight to prevent
CO2 leakage
Outer door
Shaker apparatus
Outer stainless door heater
-prevent condensation and decontamination.
CO2 inlet
Safety switch
Power cord
Fuse holder
NOTE
1. The figure 1 is to show you the name of each part of exterior incubator.
2. The diameter of blue PVC tube that we use for inflow of gas is 6mm. (total diameter 6mm, hole 4mm)
Connecting Power Cord.
1. Verify your supply voltage matches the voltage of your incubator
2. Insert the power cord into its receptacle.
3. Plug the cord into power supply outlet.

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4. FEATURES AND SPECIFICATIONS OF THE UNIT
4.1 Features
1. Excellent Uniformity of Temperature, CO2.
2. 6 Side Direct Heat for Temperature Uniformity and Fast Recovery
3. IR CO₂Sensor detects precise density of CO₂
4. Outer Heated Door ensures no condensation on glass door.
5. Microprocessor PID control for Temperature, CO₂
6. Natural Humidification System by water tray and circulation fan.
7. Simple operation for Shaker apparatus.
4.2 Specification
Items
NB-203QS
Temperature
Range
Ambient +5℃to 60℃
Accuracy
±0.25℃at 37℃
Controller
Microprocessor Digital PID
Humidity
70% ~ 80% at 37℃
CO2
Range
0% to 20%
Accuracy
±0.1% at 5% / 37℃
CO2 Increment
0.1%
CO2 Sensor
IR CO2 Sensor
Shaker
RPM
30 to 300 rpm
RPM Accuracy
±1rpm
Increment
1rpm
Orbital throw
22mm
Motor
BLDC (Plate Type Brushless DC Motor)
TIME Range
Continuous or up to 99hrs 59min
Platform
300 x 330(mm) with Silicon Rubber Pad
Outer door
Silicon Packing Magnet Door
Inner door
Tempered Safety Glass Door
Jacket type
Dry wall type (6 sides heat)
Chamber material
Stainless Steel (304)
Weight
70kg
Capacity / Shelve
179 liter / 1 shelf
Chamber dimension
473 x 528 x 710(H)mm
Overall dimension
560 x 665 x 945(H)mm
Power
110/220V, 50/60Hz, 550W

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5. Control Panel
1 2 3 4 5 6 7 8 9
17 16 15 14 13 12 11 10
1. Alarm Mute : Alarm mute switch
2. SAFETY ACTIVATION INDICATION LAMP: If Safety is activated, it will be ON
3. Heating Display : To show status of heating activation at 3 parts where is controlled by 3 each
sensors. Glass door means the heater around glass door.
4. Temperature display LED window
5. CO₂Gas % display window
6. Pilot Lamp for CO2 valve: Pilot lamp ON position means inflow of gas
7. Shaker activation lamp : this lamp blinks when shaker runs.
8. RPM display : RPM value display
9. Alarm button : Alarm ON OFF switch
10. Start/Stop button
11. Adjustment button
12. SET button
13. Mode key : RPM/TIME set key
14. Calibration button
15. CO₂Gas Setting Button
16. Adjustment button
17. Temperature set button

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6. OPERATION
6.1 POWER S/W ON
Press the POWER S/W on the right side of the product.
The CO2 Control Panel Display will be shown as follows
6.2 CO2 INCUBATOR
6.3 Connect CO2 Cylinder
Note : Prior to connect the power plug, make sure that the POWER S/W is off.
Before Switch ON, insert water tray filling with distilled water. Make sure connection of gas supply. And
Open CO2 gas cylinder or supply with the pressure of regulator set to 4.5Psi or 0.3 bar.
Note : The pressure gage may be difference according to its manufacturing companies respectively.
If Regulator's pressure is too high, it may cause malfunction of the CO2 control.

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6.4 Turn on the power switch
The digital readout of current temperature and density of CO2 & temp. in chamber will be displayed.
6.5 Temperature & CO2setup
6.5.1 SETTING TEMPERATURE:
a. Press the "TEMP/SET" key, Then, LED screen will flicker continually.
b. Input the desired temperature by pushing UP (▲) and DOWN (▼).
c. Press "TEMP/SET" key again after input. “SAVE” is shown up on LED screen as below
After set-up, LED screen will stop flickering.
* If you don't press "SET" key lastly after adjusting, the new set-up value will not be
saved at all.
* Set-up Temperature range is Ambient +5℃~ 60℃(Normal)
Note: The initial set point for temperature is at 37C from N-BIOTEK facility.
6.5.2 SETTING CO2 DENSITY:
a. Press "CO2SET" key. Then, LED screen will flicker continually.
b. Input the desired value of Co2 density by pushing UP (▲) and DOWN (▼) key
c. Press "SET" key again after input. “SAVE” is shown up on LED screen like below.
After set-up, LED screen will stop flickering.
* If you don't press "CO2SET" key again after set-up, the new set-up value will not be
saved at all.

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6.6 CALIBRATAION OF TEMPERATURE & CO₂:
Please follow up below procedure for calibration in case of discrepancy between actual
value (measured by reliable measurement device) in chamber and displayed value.
Measure CO₂density and Temperature after incubator is stabilized in which takes about more than
2 hours (you might want to perform this stabilization process at night before home)
Please note that low deviation range such as ±0.1 ~ 0.3% may not be corrected precisely by this
calibration.
No.
DISPLAY
FUNCTION
1
Chamber Temperature
2
Door Heater Temperature
3
Glass Door Heater Temperature
4
CO₂Deviation Calibration
5
Heating On Off Range Control
(Factory Setup Only, DO NOT CHANGE)
6
CO₂gas supply speed control
(Factory Setup Only, DO NOT CHANGE)
7
To apply a new value
a. Press and hold “CAL/SET”for 10 seconds. Then, LED will be flickering as below.
Channel 1 is at chamber’s Main Temp calibration stage.
Press UP (▲) as much as difference from measured value by precise analyzer if it is higher.
Press DOWN (▼) as much as difference from measured value by precise analyzer if it is lower.
Ex) If measured temperature is 38℃and Display shows 37℃, then press up 1℃.
Note
* Calibration range for temperature is ±5℃
* To go to next channel is to press “CAL/SET”button. After 5th channel, the LED is back to
temperature display.

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b. Second Click “CAL/SET” ☞ Outer door’s Temp calibration
Channel 2 is purposed to remove water condensing on glass door caused by high temperature
difference between chamber and outside. Recommend to use calibration at Channel 2 in case of
water condensing on glass door.
Note
Except water condensing on glass door, calibration of channel 2 and 3 is not recommendable.
Check if the water condensing is removed in 3 Hours after calibration of CH2 is done.
c. Third Click “CAL SET” ☞ Door Frame Heater calibration
Channel 3 is purposed to remove excessive water condensing on glass door caused by high
temperature difference between chamber and outside. When failed to remove water condensing by
calibrating CH2, try to calibrate
d. Fourth Click “CAL SET” ☞ CO2 density calibration
Channel 4 is at CO₂density calibration stage.
Press UP (▲) as much as difference from measured value by precise analyzer if it is higher.
Press DOWN (▼) as much as difference from measured value by precise analyzer if it is lower.
Ex) If measured CO₂value is 5% and Display shows 4%, then press up 1%.
e. Fifth Click "CAL SET" ☞ Heating control
* This is pre-programmed mode before releasing from manufacturer.
Push UP (▲) and DOWN (▼) to set the value

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NOTE
Channel 5 is to set heating control point.
If it is set at 3, then the heating control works from 34’C against setting value i.e. 37’C.
This is in order to minimize overshooting and faster reaching time to setting value.
Therefore, user is kindly required not to change this value.
f. Sixth Click "CAL SET" ☞ CO2 gas supply control.
* This is pre-programmed mode before releasing from manufacturer.
Push UP (▲) and DOWN (▼) to set the value
NOTE
Channel 6 is to set starting point of solenoid control for CO₂ supply.
Factory pre-programmed set point is at 2 (2 for NB203XL, 3 for NB203) to optimize set-up at 5% and it means
that CO₂control valve is in open position until the CO₂density reaches to 3%. From 3% of CO₂ to setting
value, solenoid valve takes control of CO₂supply until it gets to setting value.
When above stage is cleared, please press the button to save the new value.

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6.7 Alarm
3 minutes after both temperature and CO2 density reach to set point, all alarm systems are activated as
programmed. If the temperature or CO2 is not reached to set point, its alarm system is not stated at all.
When turning off alarm switch {Location “l”}, all alarm system is not worked
Alarm Activation
Temperature: ±2℃ from set point
CO2: ± 1% from set point
Door Open: 1 minute after door opening.
*When pressing the alarm mute button on the control panel will stop the alarm from activating.
If the alarm was stopped by mute button, the audible alarm (except alarm for the door) will be re-activated
every 10minutes from the initial alarm if either or both CO2 and Temperature is still in alarm range.
This is to remind user of the error which continued and not corrected.
7. Safety Switch
It is the safety device to prevent the heater from overheating when the
temperature controller is malfunctioning.
■ Set the Safety S/W higher than setting point.
■The Safety S/W has wide deviation
■ Safety S/W is the safety device for preventing the heater to overheat
when TEMP. CONTROL is malfunctioning.
This is the safety device for preventing temperature from rising caused by any circumstances.
So set the temperature at 5℃higher than operating temperature or desired point.
■ You can identify its activation by checking lighting of pilot lamp.
■It is set at 40℃~ 45℃when released. but it may be changed during transportation.
Therefore, please check and set desired point.

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8. Shaker Operation
8.1 Connecting the Shaker
After cleaning the surface of chamber or separating the shaker, please connect it as below.
(The shaker is already well fitted when released. it must be followed to turn off power when disconnect
or connect the shaker as below)
8.2 RPM, TIME, PARAMETER Set up
8.2.1 TIME set up
a. Power S/W ON : Below is when turning on the main switch.
b. When you press the “RPM/TIME” button, t 00.00 is displayed
and you need to press the “SET” button and check the display flickers at this moment
c. During the display flickers, you can set the hour through pressing “(UP)▲(DOWN)▼” keys
and after setting the hour, you can set the minute through the same way with setting the hour.
d. Press the “SET” key after setting the time (hour and minute),
then the screen displays “t SAVE” as below.
8.2.2 RPM set up
a. After the time setting is completed, press the “RPM/TIME” button again,
b. then the display will show the original RPM value.
c. Press the “SET” button, then RPM display will flicker.
d. You can set the RPM through pressing “▲,▼” keys during the display is flickering.
e. After setting the desired value, press the “SET” key again then,
the screen displays “r SAVE” as below
After Setting the RPM is also completed, press the “START/STOP button”,
then the product will operate as saved time and RPM.

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* NOTE
RPM MODE
➢To set RPM is possible when not running.
➢RPM range is from at from 30 to 300 RPM.
➢Setting RPM below 30 can cause error.
➢Press set button after changing setting value for saving new value.
TIME MODE
➢To set TIME is possible when not running.
➢TIME range is from t 00.00 or up to 99hrs 59min.
➢In order to save the new value, press set button after changing value.
➢Operation will stop when TIME ends. and -END- will be displayed.
➢At this stage, to press START/STOP Button is to resume the TIME function same as before.
If you need another TIME setup, re-arrange the setting.
➢The remaining TIME will be on display when press RPM/TIME Button during RPM mode
9. How to detach the Shaker
When the product is needed for cleaning, it is hard to wipe everywhere in the chamber without
detaching shakers and shelves. shaker and shelves in chamber are able to be detached easily.
Note: Make sure to turn off the product first prior to disconnect the roller from the product.

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10. Troubleshooting
Fault description
Possible cause
Recommended fix
Heating
Chamber heating permanently,
set point not held
SSR relay defective
Replace SSR relay
Control panel display defective
Replace display screen
Temperature sensor defective
Replace temperature sensor
Chamber does not heat up.
SSR relay defective
Replace SSR relay
Power not supplied to heating
circuit
Reconnect the power plug on
the panel.
Contact N-BIOTEK service
Unit does not switch on
(main switch is in position “l”)
The miniature fuse has blown.
Replace the fuse with type
5x20mm, 220V(5A),110V(7A).
If the newly inserted fuse
triggers again, there is short
circuit: contact N-BIOTEK
service.
Switch defective
Replace the switch
Gas
CO2 concentration in chamber
is too high/ too low.
Defective function of the CO2
controller
Reset the alarm.
CO2 sensor system defective
Contact N-BIOTEK service.
The concentration of CO2 or
O2 does not reach the adjusted
set value.
Gas inlet defective
Replace the gas tube
Gas leaking from inner tube
connecting region.
Replace the inner tube.
Solenoid valve defective.
Replace solenoid valve
Humidity
Condensations
inside the chamber
Fan defective
Replace the fan.
(contact N-BIOTEK service)
Condensation on the door
Improper temperature
distribution b/w the door and
the chamber
Increase the value of door
heating temperature. (Ch.2)
No or too low humidity inside
Water pan empty
Fill the water pan with distilled,
sterile water.
Roller
Not running roller
Main board defective possibly
Replace main board
Not proper engaged with axis
and shaft
Make sure to fix both axis and
shaft in.
Manufactured by : N-BIOTEK, Inc
Techno park 402-803 Yakdae-Dong, Wonmi-Gu, Bucheon-si Gyeongi province Korea, Republic of
Tel : [phone] E-mail : [email protected]