New Era Pump Systems Multi-Phaser NE-1000 Series Programming manual

Publication #1200-03 Revision 16 V3.74 2/10/09
New Era
Pump Systems Inc.
WWW.SYRINGEPUMP.COM
Multi-Phaser™
Model: NE-500 / NE-501
NE-1000 Family of Programmable Syringe Pumps
0 10 20 30 40 50 60 70 80 90
0
10
20
30
40
50
60
Time
InfusionRate
“WHAT’S YOUR APPLICATION?”™
WARNING
WARNINGWARNING
WARNING
NOT FOR CLINICAL
NOT FOR CLINICALNOT FOR CLINICAL
NOT FOR CLINICAL
USE ON HUMANS
USE ON HUMANSUSE ON HUMANS
USE ON HUMANS

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Quick Start Instructions
Assumes that the pump was not previous programmed with a multiple Phase Pumping Program.
•Plug in the pump.
•Attach the computer cable to the "Computer" connection on the RJ-11 connector
•Start the terminal emulator program on the computer (downloadable from the web site).
Baud Rate: 19,200
Data frame: 8N1 (Default settings for the downloadable terminal emulators)
•Note: Each command sent to the pump must have a carriage return (0x0D) at the end, but space characters are
optional and will be ignored. (Sent automatically by terminal emulator).
•Note: Wait for the response from each command before sending the next command
Setup Pumping Parameters
Set the Syringe Inside Diameter:
•Where "nn.nn" is the syringe inside diameter in mm: Send the command: DIA nn.nn
Set the Pumping Rate.
•Where "nn.nn" is the pumping rate and "xx" is the rate's units: Send the command: RAT nn.nn xx
Rate units "xx" value
mL/hr MH
µL/hr UH
mL/mn MM
µL/mn UM
•If the pumping rate is out of range the response message to the computer will include "?OOR"
Set the Volume to be Dispensed or Continuous Pumping
•Where "nn.nn" is the volume to be dispensed, send the command: VOL nn.nn
If the volume to be dispensed is 0, then the pump will pump continuously.
•Note: The default units for the volume to be dispensed will be mL or µL, depending on the syringe size.
At or below 14.00 mm syringe diameter, volume units are µL.
Above 14.00 mm syringe diameter, volume units are mL. The default volume units can be overriden.
Set the Pumping Direction
•For infusion, send the command: DIR INF
For Withdrawal, send the command: DIR WDR
Load the Syringe
•Press in the white drive-nut button to move the pusher block.
•Insert the syringe plunger in the pusher block slot.
•Insert the syringe barrel flange in the flange brackets with the syringe barrel holder on the syringe. Tighten the
flange brackets onto the syringe flange. Tighten the pusher block screw.
Operating the Pump
Start the Pump: Send the command: RUN
Stop the Pump: Send the command: STP
Query the Pump Status
•Send just a carriage return to the pump. The pump will respond with a status prompt
When Pumping
•The pumping rate can be changed.
•With continuous pumping, the pumping direction can be changed.
PUMP RESET:
PUMP RESET:PUMP RESET:
PUMP RESET: Send the command: *RESET

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Table of Contents
1. GENERAL INFORMATION........................................................................................... 1
1.1 W ARNINGS
!
AND C AUTIONS
!
.............................................................................. 1
1.2 D ISCLAIMER .................................................................................................................... 1
1.3 W ARRANTY ..................................................................................................................... 1
1.4 P ACKING L IST ................................................................................................................. 2
2. OVERVIEW ...................................................................................................................... 2
2.1 G LOSSARY OF T ERMINOLOGY AND C ONCEPTS ............................................................ 3
3. SETUP ................................................................................................................................ 4
4. LOADING SYRINGES..................................................................................................... 5
5. OPERATION..................................................................................................................... 5
5.1 S YRINGE I NSIDE D IAMETER ........................................................................................... 6
5.1.1 D EFAULT V OLUME U NITS ......................................................................................... 6
5.1.2 C HANGING V OLUME U NITS ...................................................................................... 6
5.2 S TART /S TOP T RIGGERS .................................................................................................. 6
5.3 O PERATING THE P UMP ................................................................................................... 6
5.4 P UMPING R ATE ............................................................................................................... 7
5.4.1 C HANGING THE P UMPING R ATE AND D IRECTION W HILE P UMPING ......................... 7
5.5 P UMPING D IRECTION ..................................................................................................... 7
5.6 D ISPENSE V OLUME T ARGET AND C ONTINUOUS P UMPING .......................................... 7
5.7 V OLUME D ISPENSED ....................................................................................................... 7
5.8 R ESUMING W HEN P AUSED ............................................................................................. 7
5.9 P UMP S TALLED ............................................................................................................... 8
6. SETUP CONFIGURATION ............................................................................................ 8
6.1 RS-232 P UMP N ETWORK C ONFIGURATION .................................................................. 8
6.2 P OWER F AILURE M ODE ................................................................................................. 9
6.3 L OW N OISE M ODE .......................................................................................................... 9
6.4 A UDIBLE A LARM E NABLE .............................................................................................. 9
6.5 TTL I/O O PERATIONAL T RIGGER D EFAULT C ONFIGURATION ................................ 10
6.6 TTL I/O D IRECTIONAL C ONTROL I NPUT C ONFIGURATION ..................................... 10
6.7 P UMP M OTOR O PERATING TTL O UTPUT C ONFIGURATION ..................................... 10
6.8 L OCKOUT M ODE ........................................................................................................... 11

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7. PUMPING PROGRAM.................................................................................................. 11
7.1 H OW TO E NTER P UMPING P ROGRAMS ....................................................................... 11
7.2 C LEARING THE P UMPING P ROGRAM .......................................................................... 12
7.3 P ROGRAM F UNCTION D ESCRIPTIONS ......................................................................... 12
7.3.1 R ATE F UNCTION ..................................................................................................... 12
7.3.2 I NCREMENT R ATE F UNCTION ................................................................................. 12
7.3.3 D ECREMENT R ATE F UNCTION ............................................................................... 12
7.3.4 S TOP P UMPING O PERATION AND E ND THE P ROGRAM ........................................... 12
7.3.5 J UMP TO P HASE ...................................................................................................... 12
7.3.6 S UB -P ROGRAM S ELECTION I NPUT ......................................................................... 13
7.3.7 S UB -P ROGRAM S TART L ABEL ................................................................................ 13
7.3.8 D EFINE S TARTING P HASE OF L OOP ....................................................................... 14
7.3.9 D EFINE C ONTINUOUS L OOP E ND ........................................................................... 14
7.3.10 D EFINE L OOP E ND AND L OOP R EPETITIONS .......................................................... 14
7.3.11 P AUSE P UMPING .................................................................................................... 14
7.3.12 J UMP TO P HASE I F E XTERNAL T RIGGER ................................................................ 15
7.3.13 S ETUP E VENT T RIGGER J UMP P HASE .................................................................... 15
7.3.14 S ETUP E VENT S QUARE W AVE T RIGGER J UMP P HASE ........................................... 15
7.3.15 S ETUP E XPANSION P ORT E VENT T RIGGER J UMP P HASE ....................................... 15
7.3.16 S ETUP E XPANSION P ORT P AUSE AND T RIGGER L EVEL W AIT P HASE .................... 16
7.3.17 S ETUP E XPANSION P ORT P AUSE AND T RIGGER E DGE W AIT P HASE ..................... 16
7.3.18 E VENT R ESET ......................................................................................................... 16
7.3.19 O VERRIDE O PERATIONAL T RIGGER D EFAULT C ONFIGURATION ........................... 16
7.3.20 S ET TTL O UTPUT P IN ............................................................................................ 16
7.3.21 S ET TTL E XPANSION P ORT O UTPUT P IN L OW ...................................................... 16
7.3.22 S ET TTL E XPANSION P ORT O UTPUT P IN H IGH ..................................................... 17
7.3.23 B EEP ....................................................................................................................... 17
7.4 P UMPING P ROGRAM E XAMPLES ................................................................................. 18
7.4.1 E XAMPLE 1: 2 S TEP R ATE ...................................................................................... 18
7.4.2 E XAMPLE 2: R EPEATED D ISPENSES WITH S UCK B ACK .......................................... 18
7.4.3 E XAMPLE 3: R AMPING THE F LOW R ATE ................................................................ 19
7.4.4 E XAMPLE 4: C OMPLEX D ISPENSES WITH E XTERNAL S YNCHRONIZATION ............ 20
7.4.5 E XAMPLE 5: C ONTROL FROM A H IGH -L OW P RESSURE S ENSOR ............................ 22
7.4.6 E XAMPLE 6: A UTOMATED D ISPENSING WITH S YNCHRONIZATION ........................ 23
7.4.7 E XAMPLE 7: D ISPENSING WITH C OMPLEX S YNCHRONIZATION ............................. 24
8. RS-232 COMMUNICATIONS....................................................................................... 25
8.1 C ONNECTION AND N ETWORKING ................................................................................ 25
8.2 RS-232 P UMP N ETWORK C ONFIGURATION ................................................................ 26
8.3 RS-232 P ROTOCOL ....................................................................................................... 26
8.3.1 RS-232 G ENERAL S YNTAX L EGEND ....................................................................... 27
8.3.2 RS-232 P ROTOCOL : B ASIC M ODE .......................................................................... 27
8.3.3 RS-232 P ROTOCOL : S AFE M ODE ............................................................................ 28
8.3.4 RS-232 P ROTOCOL : B ASIC AND S AFE M ODE C OMMON S YNTAX .......................... 29
8.3.5 N ETWORK C OMMAND B URST ................................................................................. 29
8.4 C OMMAND E RRORS AND A LARMS .............................................................................. 30
8.5 RS-232 C OMMAND S ET ................................................................................................ 30

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8.5.1 P ROGRAM F UNCTION C OMMANDS .......................................................................... 30
8.5.2 P UMP O PERATIONAL C OMMANDS .......................................................................... 33
8.5.3 C ONFIGURATION AND S ETUP C OMMANDS .............................................................. 33
8.5.4 G ENERAL C ONTROL AND S TATUS C OMMANDS ...................................................... 34
8.5.5 S YSTEM C OMMANDS .............................................................................................. 35
8.6 G ETTING S TARTED W ITH RS-232 ............................................................................... 36
9. LOGIC INTERFACE: TTL INPUT AND OUTPUT ................................................. 37
9.1 TTL I/O O PERATIONAL C ONTROLS ............................................................................ 38
9.2 TTL I/O C ONTROL FROM THE P UMPING P ROGRAM ................................................. 40
9.3 TTL I/O C ONTROL FROM RS-232............................................................................... 40
10. APPENDIX ...................................................................................................................... 41
10.1 RS-232 C OMMAND S UMMARY ..................................................................................... 41
10.2 RS-232 P UMP N ETWORK C ONNECTOR W IRING ........................................................ 42
10.3 A CCESSORIES ................................................................................................................ 42
10.3.1 ANA-BOX .......................................................................................................... 42
10.3.2 S YRINGE H EATER ................................................................................................ 42
10.3.3 RS-232 N ETWORK C ABLES ................................................................................. 42
10.3.4 A UTOMATION C ABLE : R ECIPROCATING /D UAL P UMPS C ONTROL C ABLE .......... 43
10.3.5 V ALVE C ONTROLLER .......................................................................................... 43
10.3.6 F OOT S WITCH ...................................................................................................... 43
10.3.7 F IRMWARE U PGRADE .......................................................................................... 43
10.4 T ROUBLESHOOTING AND M AINTENANCE ................................................................... 43
10.5 S PECIFICATIONS ........................................................................................................... 44
10.5.1 M ECHANICAL & E LECTRICAL ............................................................................. 44
10.5.2 O PERATIONAL ..................................................................................................... 44
10.6 C USTOM A PPLICATIONS .............................................................................................. 44
10.7 S YRINGE D IAMETERS AND R ATE L IMITS ................................................................... 45

New Era Pump Systems Inc. www.SyringePump.com Model NE-500/NE-501 Multi-Phaser™
Publication #1200-02 102/10/09
1. General Information
Thank you for purchasing the NE-500 / NE-501 OEM Programmable Syringe Pump. With the NE-500 /
NE-501 syringe pump, you will be able to perform simple infusions or implement a complex automated
dispensing system.
Please familiarize yourself with the NE-500/501’s operation by reading this user's manual. For future
reference, record the serial number, located on the rear of the pump, and the date of purchase.
New Era Pump Systems Inc., located in Farmingdale, NY USA, can be contacted at:
Phone: [phone] FAX: [phone] Email: [e-mail]
WWW.SYRINGEPUMP.COM
This Operating Manual, and the NE-500/NE-501’s hardware, electronics and firmware are copyrighted.
Copyright 2004-2008, all rights reserved.
1.1 Warnings
!
and Cautions
!
!
Read the user’s manual
!
No user serviceable parts are inside.
!
Disconnect power from the pump when connecting or disconnecting cables.
!
Do not immerse the pump in liquid
!
Install on a stable surface.
!
Keep hands and loose clothing away from the pumps moving parts.
!
The pump can automatically start when the Pumping Program is operating or when attached to an
external control device.
!
Prevent liquids from entering openings of the pump.
!
Do not push objects of any kind into the chassis openings, except for appropriate cables and
connectors.
!
If the pump becomes damaged, do not use unless certified safe by a qualified technician. Damage
includes, but is not excluded to, frayed cords and deterioration in performance.
!
Discharge static from control cables before connecting by touching the cable to ground.
!
Before touching the pump, discharge static by touching ground.
1.2 Disclaimer
New Era Pump Systems Inc. makes no representations or warranties, expressed, statutory or implied,
regarding the fitness or merchantability of this product for any particular purpose. Further, New Era Pump
Systems Inc. is not liable for any damages, including but not limited to, lost profits, lost savings, or other
incidental or consequential damages arising from ownership or use of this product, or for any delay in the
performance of its obligations under the warranty due to causes beyond its control. New Era Pump
Systems Inc. also reserves the right to make any improvements or modifications to the product described in
this manual at any time, without notice of these changes.
New Era Pump Systems Inc. products are not designed, intended, or authorized for use in applications or
as system components intended to support or sustain human life, as a clinical medical device for humans,
or for any application in which the failure of the product could create a situation where personal injury or
death may occur.
All brand and product names used in this manual are the trademarks of their respective owners.
1.3 Warranty
New Era Pump Systems Inc. warranties this product and accessories for a period of two years, parts and
labor, from the date of purchase. The repaired unit will be covered for the period of the remainder of the
original warranty or 90 days, whichever is greater.
A return authorization number must be obtained from New Era Pump Systems Inc. before returning a unit

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for repair. Warranty covered repairs will not be performed without a return authorization number. At the
option of New Era Pump Systems Inc., a defective unit will be either repaired or replaced.
This warranty does not cover damage by any cause including, but not limited to, any malfunction, defect or
failure caused by or resulting from unauthorized service or parts, improper maintenance, operation
contrary to furnished instructions, shipping or transit accidents, modifications or repair by the user, harsh
environments, misuse, neglect, abuse, accident, incorrect line voltage, fire, flood, other natural disasters, or
normal wear and tear. Changes or modifications not approved by New Era Pump Systems Inc. could void
the warranty.
The foregoing is in lieu of all other expressed warranties and New Era Pump Systems Inc. does not assume
or authorize any party to assume for it any other obligation or liability.
1.4 Packing List
The OEM Starter Kit for the NE-500 / NE-501 Multi-Phaser™ Programmable Syringe Pump includes the
following items:
•One of the following external unregulated power supply adapters:
Input: One of: 120V AC 60 Hz, 220V AC 50 Hz, 240V AC 50 HZ,
or other custom specified power supply
Output: 12V DC @ 800 mA
•Primary Computer RS-232 interface cable: CBL-PC-PUMP-7
•This Operating Manual
2. Overview
The NE-500/NE-501 is a general purpose single syringe pump capable of infusion and withdrawal. It is
controlled from a microcontroller based system which drives a step motor, allowing a large range of
pumping rates calibrated to the inside diameter of the loaded syringe. The syringe is driven from a drive-
screw and drive-nut mechanism.
Features:
♦Infusion and withdrawal pumping of syringes up
to 60 cc.
♦Pumping rates from 0.73 µl/hr with a 1 cc
syringe to 2120 ml/hr with a 60 cc syringe.
♦NE-501 only: Stall detection: Automatically
stops pump when pumping is impeded
♦Infusion and withdrawal volumes separately
accumulated.
♦Programmable dispense volumes.
♦Programmable Phases allowing complex
pumping applications and interaction with
external devices.
♦Program Sub-Programs, selectable by the user.
♦Non-volatile memory of all operating
parameters and Pumping Program
♦RS-232 bi-directional control from a computer.
♦Built-in pump network driver. Pump network
supports up to 100 pumps and other devices.
♦Two modes of RS-232 control, Basic and Safe.
Safe mode provides communication error
detection, loss of communication detection, and
automatic transmitting of alarm conditions.
♦TTL logic I/O with firmware filtered control
inputs to eliminated glitches and ringing on the
control inputs.
♦Configurable and programmable TTL
operational trigger for flexible logic control.
♦Power Failure Mode: Restarts the Pumping
Program after a power interruption.
♦Audible Alarm
♦Many more features!

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2.1 Glossary of Terminology and Concepts
When a device has as many features as the NE-500/NE-501, understanding its operation could be a
daunting task at first. By understanding the key concepts and terminology used in this manual, the
operation of the NE-500/NE-501 will become quite intuitive. Every effort has been made to design the
NE-500/NE-501 with a consistent and intuitive user interface.
To facilitate and enhance your understanding of the NE-500/NE-501's operation, please take the time to
familiarize yourself with the basic concepts below:
Parts of the Pump
Parts of the PumpParts of the Pump
Parts of the Pump
1) End Plate
2) Anti-Siphon Plate Adjustment Knob
3) Drive-Nut Button
4) Anti-Siphon Plate
5) Drive-Screw
6) Syringe Retainer Thumbscrew (2, one on
each side)
7) Syringe Retainer Bracket
8) ‘V’ Slot (on Syringe Holder Block)
9) Syringe Holder Block
10) Syringe Holder Clamp
11) Guide Rod (2 guide rods)
12) Pusher Block
Parts of a Syringe
Plunger
Flange
Plunger
Barrel
Barrel Flange
3
4
5
6
7
8
1
2
9
10
11
12

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Terminology
TerminologyTerminology
Terminology
Pumping Program: The sequence of automated operations entered into the pump. This could be
as simple as a single function to pump at a single infusion rate continuously.
Pumping Program
Operating:
When the pump is started with the RUN command, or any other source, the
pump begins performing the operations in the Pumping Program until the
Pumping Program either stops automatically or the pumped is stopped with
the STP command, or from any other source. While performing the
operations defined in the Pumping Program, the Pumping Program is referred
to as operating.
While Operating, the motor can be pumping or stopped, according to the
Pumping Program.
Pumping Program
Stopped:
The motor is stopped and the pump is not operating the Pumping Program.
Pumping Program
Paused:
The Pumping Program has been stopped, but can be resumed at the point
where it was stopped.
Pumping Program
Resumed:
Continuing a Pumping Program that was Paused before the completion of the
Pumping Program. The Pumping Program continues at the point where the
Pumping Program was stopped.
Executed: The pump has performed a single operational Phase as defined in the Pumping
Program.
Program Phase: A single defined operation in the Pumping Program.
Phase Number: A Program Phase’s numerical sequence location in the Pumping Program.
Currently Selected
Function:
Each Pumping Program Phase instructs the pump to perform a particular
operation. Only one Program Phase is selected at any one time. This is the
current Phase. Each Phase is set to one function. The set function of the
current Phase is the currently selected function.
Pumping Rate
Function:
Each Pumping Program function instructs the pump to perform a particular
operation. If the Phase’s operation instructs the NE-500/501 to pump, then
associated with that Phase is the Phases’ pumping information. When a
Program Phase is selected, the ‘Rate’, ‘Volume’, and pumping direction refer
to the currently selected Program Phase’s function. The Program functions
that are associated with pumping information are referred to as Pumping Rate
functions.
Function
Parameter:
Certain functions, which do not instruct the NE-500/501 to pump, require
additional data. This additional data, sent with the function command, is the
function’s parameter.
Start Trigger: The Pumping Program may be started, or stopped, from multiple sources.
These are the TTL I/O ‘Operational Trigger’ input, or from a command
received through the RS-232 connection.
3. Setup
♦Place the pump on a stable surface.
♦Plug the connector end of the power supply into the power plug located on the PCB on the side of the
pump. See section 9, Logic Interface: TTL Input and Output, for a diagram of the connectors on the
pump. After power is applied to the pump, the buzzer will beep.

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♦Attach the pump’s serial port to the computer’s serial port using the cable CBL-PC-PUMP-7. Attach the
RJ-11 (Telephone connector) to the pump’s “Computer” connector. Attach the DB-9 connector to the
proper connector on the computer. Note: Cable is compatible with USB to RS-232 converter cables.
♦Note on USB: If an RS-232 port is not available on your PC, the pump may be operated through a USB to
RS-232 converter cable (available as an accessory, part #CBL-USB232). This cable attaches to the
standard RS-232 to PC cable (part #CBL-PC-PUMP-7). The PC will create a virtual RS-232
communications port that communicates through the USB to RS-232 converter cable. The terminal
emulator can communicate through this virtual port.
♦Start the PUMPTERM emulator communications program. (Downloadable from web site).
♦Next the Pumping Program can be entered. Before the Pumping Program can be operated, the pump needs
the measurement of the inside diameter, in millimeters, of the syringe that will be loaded. The syringe
diameter is entered using the "DIA" command.
♦Finally, the syringe can be loaded and the pump started.
4. Loading Syringes
The syringe is loaded by securing the barrel and the pusher flange as follows:
1: Loosen the 2 thumbscrews on the syringe retainer bracket.
2: Press in fully the white drive-nut button on the pusher block, releasing the block. Taking care not to
drag the drive-nut on the drive-screw, slide the block away from the syringe holder, providing sufficient
space for the loaded syringe. Then release the white button.
3: Lift the syringe holder above the syringe holder block. Turn it 1/4 turn and then lower it onto the
syringe holder block. The syringe holder should be out of the ‘V’ slot.
4: Load the syringe with the barrel over the syringe holder and the syringe plunger towards the middle of
the pump. Place the barrel on the syringe holder, in the ‘V’ slot, with the barrel flange inserted between
the syringe holder block and syringe retainer bracket.
5: On the pusher block, turn the thumbscrew to make the slot large enough for the plunger flange. Press in
fully the white drive-nut button on the pusher block, releasing the pusher block. Then slide the block
towards the syringe plunger. Place the syringe plunger flange into the slot and against the anti-siphon
plate. When the flange is positioned in the slot, release the white drive-nut button.
6: Lift the syringe holder to slightly above the height of the syringe barrel and turn the syringe holder 1/4 turn
back to its original position and then lower it onto the syringe barrel.
7: Firmly push in the syringe retainer bracket against the syringe barrel flange and tighten the 2 thumbscrews
on the syringe retainer bracket. On the pusher block, turn the thumbscrew to tighten the plate against the
plunger flange.
⇒To unload the syringe, reverse the instructions for syringe loading.
5. Operation
Before the pump can be operated, the pumping data must be setup. At minimum, the syringe
inside diameter and a non-zero pumping rate needs to be set. The operation of the pump can
then be started from the TTL I/O connector, or from RS-232 computer control.
All data that is set will be stored in the pump’s non-volatile memory, meaning that the new
value will not be lost the next time that power is applied to the pump. The only exception is when the pumping
rate is changed while the Pumping Program is operating. In this case the new pumping rate will not be stored
in non-volatile memory.
In general, numeric data are floating point data consisting of up to 4 digits plus 1 decimal point, and is referred
to in the command descriptions as <float> data. For example 123.4, 12.3 and 12.34 are acceptable formats for
floating point data.

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5.1 Syringe Inside Diameter
Set with command: DIA <float>
The syringe inside diameter can only be set while the Pumping Program is stopped.
Valid syringe diameters are from 0.1 mm to 50.0 mm. If the diameter is out of this range, the response will be
‘?OOR’. Changing the syringe diameter will not zero any current settings. Section 10.7, “Syringe Diameters
and Rate Limits”, is a representative list, for reference, of syringe diameters for various syringe manufacturers
and syringe sizes.
5.1.1 Default Volume Units
The units of the accumulated infusion and withdrawal volumes and the “Volume to be Dispensed” are set
according to the diameter setting. NOTE: A change in the volume units will affect all “Volume to be
Dispensed” settings in the Pumping Program. If the default volume units are changed (see next section), the
selected volume units will remain in affect until a reset function is performed.
From 0.1 to 14.0 mm Syringes smaller than 10 mL: Volume units are ‘µL’
From 14.01 to 50.0 mm Syringes greater than or equal to 10 mL: Volume units are ‘mL’
5.1.2 Changing Volume Units
The volume units used for accumulated volumes and the “Volume to be Dispensed” settings can be changed to
either ‘mL’ or ‘µL’. A change in the volume units will affect all “Volume to be Dispensed” settings in the
Pumping Program.
Set the volume units command:
VOL {ML | UL}
The selected volume units will remain in affect and override the default volume units. Changing the diameter
will no longer change the volume units. Performming a system reset (*RESET) will cancel the override and
allow the volume units to change to the default volume units when setting the syringe diameter.
5.2 Start/Stop Triggers
Start Command: RUN
Stop Command: STP
The Pumping Program can be started or stopped from different sources. The “RUN” and “STP” commands, or
the TTL I/O Operational Trigger input. Each can control the Pumping Program’s operation.
5.3 Operating the Pump
When a start trigger is given, the Pumping Program begins to operate, starting with Phase 1. If the current
Program Phase specifies a pumping rate, the pump will begin pumping. The pumping direction will also
depend on the Program Phase setup. The volume dispensed can be queried with the 'DIS' command.
While pumping, the pump will pump continuously in the current Program Phase, unless a “Volume to be
Dispensed” is set, or an Event trigger is set. If a “Volume to be Dispensed” is set, the Program Phase will be
complete after the set volume has been infused or withdrawn, measured from the start of the Phase.

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5.4 Pumping Rate
Command: "RAT [<float> [<units>]]"
Sets/queries the pumping rate and rate units.
5.4.1 Changing the Pumping Rate and Direction While Pumping
Command: "RAT <float>"
Except with some complex Pumping Programs, the pumping rate can be changed while the pump is operating.
To change the pumping rate while pumping, use the 'RAT' command to send a new rate. A new rate set while
pumping will not be stored in non-volatile memory. Rate units cannot be changed while pumping.
If the new rate is valid, it will take affect immediately.
The pumping direction can be changed while pumping if the “Volume to be Dispensed” is 0.0. The direction
command "DIR" will immediately change the pumping direction and store the pumping direction in the current
Program Phase. Also changing the pumping direction changes the accumulated “Volume Dispensed”
according to the new pumping direction.
5.5 Pumping Direction
Command: "DIR [INF | WDR]
Sets/queries the direction of pumping to Infuse (INF) or Withdraw (WDR)
5.6 Dispense Volume Target and Continuous Pumping
Command: "VOL [<float>]
To set a dispense volume, use the "VOL" command. The units of volume are set according to the syringe
diameter to either µL or mL. The current Pumping Phase will continue to pump, infusing, or withdrawing, until
the set volume has been pumped.
For continuous pumping, set the volume target to 0: Command "VOL 0".
5.7 Volume Dispensed
Volume is computed based upon the syringe inside diameter setting. The volume is accumulated separately
for infusion and withdrawal. The volume dispensed for infusion and withdrawal can be queried with the "DIS"
command.
The “Volume Dispensed” accumulations, for infusion and withdrawal, are reset to 0 when:
A) The pump is powered on.
B) The syringe diameter is changed.
C) From the RS-232 clear “Volume Dispensed” command (CLD).
D) The accumulated Volume Dispensed rolls over from 9999 to 0.
E) A sub-program is selected when the Pumping Program executes a Sub-Program Selection
function.
5.8 Resuming When Paused
If the Pumping Program is stopped before the completion of the Pumping Program, the response prompt will
change to 'P', indicating that the Pumping Program is paused. While paused, starting the pump again will
resume the Pumping Program where it was stopped. This means that the Pumping Program will continue at the
point in the Phase where it was stopped and the ‘Volume to be Dispensed’ will still be referenced from when
the Program Phase first started.

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Pause is exited by sending a stop command "STP", or changing any setting. When the Pumping Program is
started again, it will start from the beginning (Phase 1).
5.9 Pump Stalled
Model: NE-501 only
When the operation of the motor is impeded due to excessive force needed to drive the syringe, the pump will
stop, pausing the Pumping Program, and a stall alarm will occur.
The buzzer will sound continuously if alarms are enabled. Also, if the RS-232 Safe Mode is enabled, an auto-
alarm message will be sent to an attached computer.
Sending any command will stop the buzzer and clear the alarm. When the problem causing the pump motor to
stall has been corrected, the Pumping Program can be resumed from any start trigger source: TTL input, or RS-
232.
6. Setup Configuration
The following configuration parameters can be set or queried:
The values for "n" are "0" for off, "1" for on. See relevant sections for command definitions.
Command Description
ADR nn Set the pump network address or mode of communications.
PF n Power Failure mode, where ‘n’ is the current setting.
LN n Low noise mode to minimize the motor’s micro-stepping resonance
AL n Alarm mode, where ‘n’ is the current setting.
TRG aa Operational Trigger default configuration, where ‘aa’ is the current configuration.
DIN n Directional control TTL logic input setup
ROM n Pump motor operating TTL logic output configuration
LOC n Lock out mode. Sets Auto-Run mode with Sub-Programs
6.1 RS-232 Pump Network Configuration
Command: ADR [ <nn> | <RECP> | <DUAL> ]
The pump can be configured to communicate either with a computer or another pump. Communications with a
computer (Address Mode) is the default setting and will be indicated by setting an address from 0 to 99.
When in the default Address Mode, up to 100 pumps can be attached to a computer in a single pump network.
The network address is defined by the 2 digits to the parameter <nn>. If only one pump is attached to the
computer, set the network address to 0 (ADR 0), which is the factory default.
Additionally, the baud rate can be set with this command. For details see the detailed RS-232 command
section.
Special communications modes:
ADR RECP Reciprocating Pumps. Sends Start/Stop, Pumping rate and reverse
pumping direction to an attached secondary pump.
ADR DUAL Reciprocating Pumps. Sends Start/Stop, Pumping rate and same
pumping direction to an attached secondary pump.
Note: Communications with a computer requires the accessory cable: CBL-PC-PUMP-7.
Communications with a secondary pump requires the accessory cable: CBL-DUAL

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In Reciprocating or Dual Pumps Modes, the secondary pump must be left in its default communications setting
of Address Mode, Address 0, and 19,200 baud rate. See the documentation with CBL-DUAL cable for more
detailed information.
6.2 Power Failure Mode
Command: "PF <n>", <n> Setting: ‘0’ = Disabled, ‘1’ = Enabled.
When enabled, if the Pumping Program was operating when power to the pump was disrupted, the Pumping
Program will automatically start operating when power is reconnected to the pump.


 CAUTION: The Pumping Program will start operating from the beginning of the Pumping Program
(Phase 1), regardless of what part of the Pumping Program was operating when the power was disrupted.
When the NE-500/501 syringe pump is used as a component in an automated infusion/withdrawal dispensing
system, a Pumping Program can be designed to automatically synchronize the pusher block at the start of the
Pumping Program. This would be accomplished using attached sensors that send signals to the Pumping
Program.
6.3 Low Noise Mode
Command: “LN <n>”, <n> Setting: ‘0’ = Disabled, ‘1’ = Enabled.
A side affect of the NE-1000’s high precision micro-stepped motor driver is a high frequency resonance sound
at very low pumping speeds. This mode minimizes this sound by reducing the micro-stepping, increasing
pulsations.
6.4 Audible Alarm Enable
Command: "AL <n>", <n> Setting: ‘0’ = Disabled, ‘1’ = Enabled.
When alarms are enabled, the buzzer will be sounded as follows:
Condition Buzzer Action
Pumping Program ended Continuous beeping
Pumping Program paused for start trigger Continuous beeping
Alarm condition, such as pump motor stalled Steady alarm
Any command sent to the pump will stop the alarm.

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6.5 TTL I/O Operational Trigger Default Configuration
Command: "TRG <aa>", <aa> as below in Settings.
Configures the default mode of the TTL I/O ‘Operational Trigger’ (pin 2) that will control the Pumping
Program’s operation. This default setting can be overridden within a Pumping Program. (See sec. 9.1, TTL I/O
Operational Controls). The 2 letter configuration Setting parameter to the right of the colon (:) is defined in
column ‘Setting’ as follows:
Setting
<aa>
RS-232 Command Setting
FUN TRG <n>
<n> =>
Name Function
FT 0 Foot Switch Falling edge starts or stops the Pumping Program
FH 1 Foot Switch Hold Falling edge starts the Pumping Program
Rising edge stops the Pumping Program
F2 2 Foot Switch
Reversed
Rising edge starts or stops the Pumping Program
LE 3 Level Control Falling edge stops the Pumping Program
Rising edge starts the Pumping Program
ST 4 Start Only Falling edge starts the Pumping Program
T2 5 Start Only Reversed Rising edge starts the Pumping Program
SP 6 Stop Only Falling edge stops the Pumping Program
P2 7 Stop Only Reversed Rising edge stops the Pumping Program
6.6 TTL I/O Directional Control Input Configuration
Command: "DIN <n>"
Configures how the TTL input ‘Pumping Direction’ (pin 3) will control the pumping direction. (See sec. 9.1,
TTL I/O Operational Controls).
Setting <n> Mode Function
0 Reciprocating Pumps Falling edge: Infuse; Rising edge: Withdraw
- Used for 2 pumps operating in opposite directions
1 Dual Pump Falling edge: Withdraw; Rising edge: Infuse
- Used with 2 pumps operating in the same direction
6.7 Pump Motor Operating TTL Output Configuration
Command: "ROM <n>"
Configures the functionality of the 'Pump Motor Operating' TTL output pin (TTL pin 7).
Setting <
n>
Mode
0
Sets the output to logic high only when the motor is operating (pumping).
Sets the output to logic low when the motor is not operating or when the Pumping Program is executing
a pause timer or is stopped
1 Sets the output to logic high when the motor is operating (pumping) or when the Pumping Program is
executing a pause timer.
Sets the output to logic low when the Pumping Program is stopped

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6.8 Lockout Mode
Command: “LOC <n>”, <n> Setting: '0' = Disabled, '1' = Enabled.
Lockout mode is useful for implementing Sub-Programs in a production setup.
Auto-Run Mode: When used in conjuction with the Sub-Program Select programming function (FUN PRI) as
Phase 1, the pump will enter Auto-Run Mode. In this mode, on power up, the Pumping Program will
immediately begin to execute and upon receiving an Operation Trigger input, the Pumping Program will
immediately begin executing the Sub-Program selected with the Expansion Port.
7. Pumping Program
A Pumping Program is simply a pre-defined sequence of actions, or functions, which guarantees consistent and
precise operation of the pump, automatically, and with or without any user intervention. A Pumping Program
can be as simple as continuous pumping at a fixed infusion rate. Or a Pumping Programs could consist of a
pumping rate and direction of pumping for a specified volume, then switch to another pumping rate. Also a
Program can interact with external devices through the TTL I/O connector, make decisions, or stop pumping
for a period of time.
Programs are broken into individual operations called Phases. Each Phase consists of a function that can be a
control function or pumping function. A pumping function, such as ‘RATE’, consists of a pumping rate,
optional “Volume to be Dispensed”, and the pumping direction.
Complex dispensing systems can be designed, involving multiple liquids, each dispensed from a different
pump, plus other equipment and sensors. Pumping Programs can be designed for each pump which enables
multiple pumps to synchronize with each other, and the other equipment and sensors, using a cable connected
to the TTL I/O connectors of each pump.
When the Pumping Program is started, either from the keypad, TTL I/O connector, or from RS-232, the
Pumping Program will begin with Phase 1 of the Program. After the completion of each Phase, the pump will
immediately start the next consecutive Phase. This linear sequence of Phases can be altered by certain
functions that direct the Pumping Program to continue operation with a different Phase number. Some
functions can change the order of operation conditionally based on external events.
7.1 How to Enter Pumping Programs
A Pumping Program can be uploaded from a computer using PUMPTERM software. A Pumping Program can
be stored in a text file and edited with any word processor, which facilitates development and maintenance of
the program. A Pumping Program generator spreadsheet is available to assist in developing a Pumping
Program and to create the text file for uploading to a pump.
Start by organizing your pumping requirements into specific actions and conditions that can then be
programmed into Phases. For more advance programming methods, common groups of Phases can be grouped
together and repeated multiple times using looping and jump functions.
The current values of the pumping rate, optional “Volume to be Dispensed”, and pumping direction, all refer to
the currently selected Phase. Use the command "PHN" to set the current phase.
To change the “Program Function” of the currently selected program phase, use the "FUN" command. When
finished sending the data for the current Phase, use the "PHN" command to select the next program phase to be
set. Now all pumping data will refer to next currently selected Phase. Data for the current Phase can now be
entered.
Continue selecting Phase numbers and entering the infusion or control setup for each Phase of the Pumping
Program. The entire Pumping Program will be stored in non-volatile memory.
Use the ‘STOP’ function (FUN STP) to stop the pump and end the Pumping Program. If the Pumping Program
does not operate the pump continuously, the last Phase of the Pumping Program must be a ‘STOP’ function
(unless the last Phase number is the maximum Phase number).

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When the Pumping Program is started, with the Operational Trigger TTL I/O input, or RS-232 “RUN”
command, the Pumping Program will begin operating from Phase 1.
Very complex dispensing Programs can be created with the Program functions available. Section 7.3 contains
a detailed description of all the functions.
7.2 Clearing the Pumping Program
It is not necessary to clear out a previous Pumping Program before entering a new program. New commands
will overwrite previous ones, and unused Program Phases will just be ignored. To clear out the pumping
program memory and reset communication parameters, send the command: *RESET
7.3 Program Function Descriptions
- Each program function must be in a separate Program Phase -
7.3.1 Rate Function
Command: "FUN RAT"
This is the most basic function to simply command the pump to dispense at a specific rate.
Defines a pumping function with a fixed pumping rate. This function defines a pumping setup consisting of the
pumping rate, optional “Volume to be Dispensed”, and pumping direction. Use the ‘RAT’, ‘VOL’, and ‘DIR’
commands to set or review the Rate Function's setup. For continuous pumping, set the “Volume to be
Dispensed” to 0.0.
7.3.2 Increment Rate Function
Command: "FUN INC"
The increment and decrement functions operate the same as the ‘RATE’ function, except that the specified rate
is added (‘INC’)or subtracted (‘DEC’) from the current pumping rate. The current pumping rate when the
function is executed is the base pumping rate for the function. If no base pumping rate exists, such as when
executing a pause function or when the Pumping Program is first started, a Program error will occur and the
Program will stop.
The pumping rate units will be the same as the base pumping rate, and therefore cannot be set, or queried, with
the pumping rate increment or decrement value. As with the ‘RATE’ function, a “Volume to be Dispensed”
and pumping direction can be specified for the increment and decrement functions.
When used within a Program loop, the pumping rate can be incremented or decremented in small step intervals.
7.3.3 Decrement Rate Function
Command: "FUN DEC"
The decrement function subtracts the specified rate from the current pumping rate. For a full description, see
section 7.3.2, Increment Rate Function
7.3.4 Stop Pumping Operation and End the Program
Command: "FUN STP"
Stops the pumping operation and stops the Pumping Program. The Pumping Program will begin at Phase 1
when started again. An implicit ‘Stop’ function is executed when the Program exceeds the maximum Phase
number during operation.
If alarms are enabled, the buzzer will beep continuously when the Pumping Program stops.
7.3.5 Jump to Phase
Command: "FUN JMP nn"
The ‘Jump’ function alters the consecutive operation of Program Phases. When executed, the Pumping

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Program will continue operation with Phase ‘nn’.
7.3.6 Sub-Program Selection Input
Command: “FUN PRI”
The Pumping Program can be broken into sections which can be selected by the user. Sub-Programs are
selected using the Expansion Connector. A rotary selector swich can be attached to the Exapnsion Connector
to create a stand-alone user interface. See Sec. 0, “Note on USB: If an RS-232 port is not available on your
PC, the pump may be operated through a USB to RS-232 converter cable (available as an accessory, part
#CBL-USB232). This cable attaches to the standard RS-232 to PC cable (part #CBL-PC-PUMP-7). The PC
will create a virtual RS-232 communications port that communicates through the USB to RS-232 converter
cable. The terminal emulator can communicate through this virtual port.
Logic Interface: TTL Input and Output” for more information about the Expansion connector and attaching a
switch.
Auto-Run Production Mode: When Phase 1 is set to Sub-Program Selection Input, and Lockout Mode is
selected, the pump will enter Auto-Run Mode. When the pump is powered on, it will immediately begin
executing the Pumping Program. When a start trigger, such as from a foot switch, the Pumping Program will
read the selected Sub-Program number from the Expansion Connecotr and begin executing the Sub-Program.
This permits a set of production dispenses to be programmed into the pump. The user then would only be able
to select from one of these production dispenses and not make any changes.
Foot Switch Trigger Feature: If the Pumping Program was Paused, and the Paused Phase was a continuous
pumping Rate function, then starting the pump from the Operational Trigger (pin 2), such as with a foot switch,
will cancel the Pause and restart the Pumping Program from Phase 1, causing the current program selection to
be re-executed.
When the Sub-Program Selection Input function is executed, the Pumping Program pauses and waits for a start
trigger. When a start trigger is received, the Sub-Program number is read from the Expansion Connector and
the selected Sub-Program begins execution. Also, the accumulated infusion and withdrawal dispensed volumes
are set to zero.
The pump searches for the selected Pumping Program Selection Label starting with the current Phase and
continuing to the end of the Pumping Program memory, then from Phase 1 until the current Phase is reached
again. If the selected Sub-Program Start Label is not found, the “OOR” (out of range) error message is send in
response to the current or next command.
If more than one Phase is defined with the same Sub-Program Start Label, then execution continues with the
first matching label encountered. More than one Program Selection Input function can be defined and placed
at any Program Phase needed.
If alarms are enabled, the buzzer will beep continuously while waiting for the start trigger.
7.3.7 Sub-Program Start Label
Command: “FUN PRL nn”
The Sub-Program Start Label function defines the start of a Pumping Program sub-program that can be selected
with a switch attached to the Expansion Connector.
After selecting the function, change '00', if needed, to a unique Program Label, from 0 to 99. See sec: 7.3.6,
“Sub-Program Selection Input”, for a full description. Place a Sub-Program Start Label, with a unique number
for each Sub-Program, from 0 to 99, at the starting Phase of each sub-program section.
When the Pumping Program encounters a Sub-Program Start Label function in normal execution, it will
interpret the label as a Stop function, stopping the pump and ending the Pumping Program.

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7.3.8 Define Starting Phase of Loop
Command: "FUN LPS"
Defines the start of a Program loop. For a full description of Program looping, see sec 7.3.10, Define Loop
End and Loop Repetitions
7.3.9 Define Continuous Loop End
Command: "FUN LPE"
Loops to the most recently executed, unpaired, ‘loop start’ Phase, or Phase 1 if none. This function allows a
section of the Program to be repeated continuously. For a full description of Program looping, see sec.7.3.10
Define Loop End and Loop Repetitions.
7.3.10 Define Loop End and Loop Repetitions
Command: "FUN LOP nn"
Repeats execution of the defined loop ‘nn’ times.
Loop starts and loop ends are uniquely paired during looping. When an unpaired ‘loop end’ function is
executed, it is paired with the most recent unpaired ‘loop start’ function executed (‘FUN LPS’). If no unpaired
‘loop start’ function exists, Phase 1 is used as an implied unpaired ‘loop start’. This pairing defines the loop
and the range of Phase numbers between the paired loop functions defines the scope of the loop.
When a ‘loop end’ function is executed, Program operation continues with the ‘loop start’ function paired with
the loop end function. There are 2 ‘loop end’ functions: Loop continuous (‘FUN LPE’) and Loop for a preset
number of iterations (‘FUN LOP nn’), indicated by ‘nn’. Each time a paired ‘loop end’ function is executed,
an iteration of the loop is complete. With the ‘FUN LOP nn’ function, after ‘nn’ number of loop iterations, the
defined loop is complete and Program execution continues with the next Program Phase after the ‘loop end’
function. The loop is then no longer defined or paired.
While executing Phases within the scope of a defined loop, another ‘loop start’ and ‘loop end’ can be paired
and become a defined loop within the scope of the first loop, which is referred to as the outer loop. The new
loop being referred to as the inner loop. The pairing of a loop within a paired loop is referred to as nesting of
loops, with each loop being one nested layer for the duration of the loops pairing. Loops can be nested for a
total of 3 layers deep. Loops can only be nested within the scope of an outer loop.
7.3.11 Pause Pumping
Command: "FUN PAS <nn | n.n>"
If ‘nn’ is non-zero, the Pumping Program will pause pumping (stops pumping) for ‘nn’ seconds. After the
pause interval, the next Program Phase will be executed. 'nn' can range from 00 to 99 or 0.1 to 9.9 seconds.
For pauses longer than the ‘99’ second maximum pause for this function, put the pause function within a
Program loop. A Program section with the following functions in consecutive Phases:
[FUN LPS] [FUN LPS] [FUN PAS 60] [FUN LOP 60] [FUN LOP 24],
will pause the Pumping Program for 24 hours.
If ‘nn’ is ‘00’ then the Pumping Program pauses and waits for a start trigger to resume the Program
After the start trigger, the Program will resume with the next Phase. The start trigger can be from any source,
the TTL I/O Operational Trigger, or from RS-232.
If alarms are enabled, the buzzer will beep continuously while waiting for the start trigger.

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7.3.12 Jump to Phase If External Trigger
Command: "FUN IF nn"
The ‘IF’ function conditionally alters the Pumping Program’s execution based on an external signal.
When executed, if the TTL I/O Program Input pin (pin 6) is low level, then the Pumping Program continues
operation with Phase number ‘nn’. Otherwise, the Pumping Program continues operation with the next Phase.
7.3.13 Setup Event Trigger Jump Phase
Command: "FUN EVN nn"
The ‘Event’ function sets a background event trap that is triggered by an external signal.
This one time background trap, or interrupt, stays set during the Pumping Program’s entire execution until it is
triggered or reset. This function has no other affect on the operation of the pump until it is triggered.
The event is triggered when either:
1) A falling edge (high to low TTL transition) occurs on the TTL ‘Event Trigger’ input (pin 4) or
2) A low level on the ‘Event Trigger’ input pin at the time when the function is executed.
When triggered, the current operation of the pump and the Pumping Program is interrupted, and the Pumping
Program immediately continues operation (jumps to) with Phase number ‘nn’.
After being triggered, the event trigger is reset. If an event trigger function is executed while another event trap
is still set, the new event trigger will replace the previous event trap.
7.3.14 Setup Event Square Wave Trigger Jump Phase
Command: "FUN EVS nn"
The 'Event Square Wave' function operates the same as the 'EVN' 'Event' function, with the exception of the
triggering conditions.
This function will trigger on either the rising or the falling edge of the TTL 'Event Trigger' input (pin 4).
Therefore, a square wave function on the inupt pin can be used to toggle the pump between 2 sections of a
Pumping Program. An example of this would be a Pumping Program that switched between a slow and fast
pumping rate, controlled by a square wave input.
7.3.15 Setup Expansion Port Event Trigger Jump Phase
Command: "FUN EVE <expansion pin>"
The ‘Event’ function sets a background event trap that is triggered by an external signal on a specific
Expansion Port connector pin. See sec. 0, “Note on USB: If an RS-232 port is not available on your PC, the
pump may be operated through a USB to RS-232 converter cable (available as an accessory, part #CBL-
USB232). This cable attaches to the standard RS-232 to PC cable (part #CBL-PC-PUMP-7). The PC will
create a virtual RS-232 communications port that communicates through the USB to RS-232 converter cable.
The terminal emulator can communicate through this virtual port.