Nippon Genetics FastGene NanoView Photometer User manual

Microvolume Spectrophotometer
FastGene®NanoView
Photometer
User Guide
Basic operaon guide
www.nippongenecseurope.eu

2021
Nippon Genecs Europe GmbH
FastGene® NanoView
User Guide
FastGene®NanoView
User Guide

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05
FastGene®NanoView User Guide Book
Introducon
Important note
Copyright
Thank you for purchasing FastGene® NanoView, an ultraviolet/visible ray spectrophotometer.
This user guide describes the details of installaon and operaon, precauons for use, accessories, and
other opons. Read this user guide carefully before using the equipment, and only use according to the
instrucons. Also, please keep this guide for future reference when using the equipment.
Please keep this user guide with the product.
Please read the safety instrucons before using the equipment, to operate the equipment safely and smoothly.
If you need to calibrate or install the product again, please contact the Nippon Genecs Europe customer
center.
If the user guide is lost or damaged, please contact the Nippon Genecs Europe customer center.
· FastGene® is the registered trademarks of Nippon Genecs Europe GmbH
· Any related material of this manual may not be modied or distributed in any form without
prior consent of Nippon Genecs Europe.
© 2021 Nippon Genecs Europe. All rights reserved.

06 FastGene®NanoView User Guide Book
Safety instrucons
Please read the safety instrucons carefully before using the equipment, to operate the equipment safely
· Comply with all warnings and cauons described in the user guide.
This user guide uses the following rules to describe warnings and cauons:
This mark indicates a potenally risky situaon, and failure to follow the
instrucon may lead to serious injury or even death.
This mark indicates a potenally risky situaon, and failure to follow the
instrucon may lead to a light injury or product damage.
This mark indicates addional informaon provided to ensure proper use
of this product.
Warning
Cauon
Note

07
FastGene®NanoView User Guide Book
Cauon
Warning
Precauons
Precauons about the installaon site
Make sure to install a venlaon system at the installaon site when using ammable and toxic samples.
· FastGene® NanoView weighs about 1.4 kg. This should be considered when installing.
· The laboratory table on which the equipment is installed should be able to support the
total weight of this device. In addion, use a stable table with a depth of at least 300 mm.
If dropped, the equipment may break.
· Avoid installaon sites exposed to corrosive gas or excessive dust. These adverse condions
can be detrimental to maintaining the performance of the equipment and can shorten the life
span.

08 FastGene®NanoView User Guide Book
Warning
Warning
Precauons
Installaon precauon
Installaon precauons
· Take acons to prevent equipment from falling in case of an earthquake or disaster.
· Make sure to check the power supply voltage, power consumpon, and frequency of the
equipment before supplying power.
· Grounding is essenal to prevent electric shock and ensure reliable operaon in case of a
sudden accident or discharge.
· Do not place heavy objects on the power cord. Keep away from hot objects.
· Do not modify the power cord in any way.
· Always wear safety gloves when using a sample that is harmful or biologically infecous.
· Do not use ammable spray near the equipment.

09
FastGene®NanoView User Guide Book
Product warranty
We provide a warranty on the product as specied below.
1. Product warranty period
2. Product warranty descripon
3. Excepons to the product warranty
For more informaon about the warranty period and range, please contact the Nippon Genecs
Europe service center.
If malfunction occurs during the warranty period due to a defect in the equipment (software,
hardware), the part will be replaced or repaired, free of charge. The same product, or free repair or
replacement, may not be possible for consumables or various accessories with operang life.
Product failure caused by the following will not be covered by the warranty, even during the
warranty period.
* If the product has documentaon such as a warranty, or a separate contract that includes terms
of the warranty, the provisions set forth in the document in queson shall be applied. For special
applicaons, the product warranty period will be set separately, if the product is manufactured
dierently from standard specicaons.
1) If the product is modified or used in an inappropriate way
2) If a service provider or person other than the company has repaired or modified the product
3) Damage to data or device, including basic software, that was caused by computer viruses
4) Damage to equipment, caused by electrical power failure or sudden voltage drop
5) Error caused by a problem other than the equipment itself
6) Failure caused by use in harsh environments such as high temperature, humidity, corrosive
gas, or strong vibration
7) Failure due to external shock including fire, earthquake, or contamination by harmful substances.

10 FastGene®NanoView User Guide Book
Contents
Ch. 1 Introducon
1-1 Introducon
1-1-1 Product introducon
1-1-2 Measurement principle
1-2 Screen descripon
1-2-1 Favorites screen
1-2-2 Adding/Removing favorites
1-3 Menu descripon
1-3-1 Measurement menu composion
Ch. 2 Basic Measurements
2-1 Pedestal Basic Use
2-2 Cuvee Basic Use
2-3 Baseline ON / OFF
Ch. 3 Nucleic acid measurement method
3-1 Measurement descripon
3-2 A260/A280 rao
3-2-1 Menu descripon
3-3 Usage
Ch. 4 Protein measurement method
4-1 Measurement descripon
4-1-1 Menu descripon
4-2 Usage
14
14
14
16
16
17
18
18
20
21
22
24
25
25
26
30
31
32

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FastGene®NanoView User Guide Book
Ch. 5 OD600 measurement method
5-1 Measurement descripon
5-1-1 Menu descripon
5-2 Usage
Ch. 6 Other
6-1 Data (View/Delete data)
6-2 Sengs
6-3 Product management
36
36
37
40
41
43

12 FastGene®NanoView User Guide Book
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13
FastGene®NanoView User Guide Book
Ch. 1
Introducon
1-1 Introducon
1-1-1 Product introducon
1-1-2 Measurement principle
1-2 Screen descripon
1-2-1 Favorites screen
1-2-2 Adding/Removing favorites
1-3 Menu descripon
1-3-1 Measurement menu composion
Ch.1

14 FastGene®NanoView User Guide Book
Note
1-1 Introducon
1-1-1 Product introducon
FastGene® NanoView is a compact, ultra-small sample analyzer with a simple yet rened design and
easy and convenient user interface (UI). This product can measure a wide range of absorbance, as it uses
a slope algorithm, an opcal path opmizaon technology. As three LEDs (260 nm, 280 nm, 600 nm) and
a single silicon photodiode are used, the product cost has been greatly reduced, while maintaining essenal
product funcons and performance. Baseline correcon can be performed using an addional LED (360 nm).
1-2-2 Measurement principle
① Lambert-Beer’s law
This law combines Lambert’s law and Beer's law related to the absorpon of light, and is used for
absorbance measurement. When the intensity of incident light projected onto the layer of material with
concentraon c in the thickness d is I0, and the intensity of light transmied through this layer is,
absorbance E is log(I0/I) and can be expressed as E=kcd. Beer's law states that absorbance is proporonal
to the thickness of the absorpon layer d, and Lambert's law states that absorbance is proporonal to
the concentraon of the absorbing material. The proporonal constant k varies according to the unit that
indicates density and length of the absorpon layer. The value of k when concentraon is indicated by w/
v % and the length of the absorpon layer is indicated by cm is called the non-absorbance or absorbance
coecient. When concentraon is indicated by mol concentraon and the length of the absorpon layer is
indicated by cm, it is called the mol absorbance coecient. In general, the concentraon c of the unknown
sample can be determined using this law, by measuring
absorbance when the absorpon spectrum is at its maximum.
② Slope Algorithm
FastGene NanoView automacally determines a sample concentraon range - low, middle, and high
concentraon - and sets the opmized opcal path, using the Slope Algorithm. "Slope Algorithm" is an
algorithm based on Beer's Law, which is performance improving technology using informaon such as
absorbance changes caused by the opcal path change. Using this algorithm, NanoView provides
high measurement performance over a wide concentraon range.
Small & Stand-Alone
No additional PC is required for this device, with its compact size of 145 x 190 mm (footprint),
1.4 kg, and main unit control system.
Ch.1

15
FastGene®NanoView User Guide Book
③ Low Sample Volume & High Sample Concentraon Range
Performs quantitative analysis using up to 2 µl sample volume. Performs quantitative analysis up to
10,000 ng/µl dsDNA. Accurate values can be automacally measured regardless of the volume error even
though path length is not separately adjusted, using the Slope Algorithm.
④ OD600
Refers to the measurement of opcal density at 600 nm. It is generally used as a test method to
describe the value of bacteria or other cells. OD600 can be measured using a standard Cuvee (10mm
path length cell).
Ch.1

16 FastGene®NanoView User Guide Book
Note
Fig. 1.1
1-2-1 Favorites screen
Preferred measurement modes can be added or deleted in Favorites for convenient use.
Up to 6 favorites can be added. * See 1-2-2 Adding/Removing favorites
[Favorites]: A measurement mode added by the user can be selected.
[Menu]: Any measurement modes supported by the equipment can be selected.
[Data]: Views, deletes, exports to USB, and prints the measured data.
[Seng]: Equipment language and me selecon, system, device check, and PC connecon
can be performed.
1-2 Screen descripon
Ch.1

17
FastGene®NanoView User Guide Book
Note
Fig. 1.2
Fig. 1.3
1-2-2 Add/Remove favorite
The icon is displayed in yellow, if the mode is added to Favorites. The icon is displayed in gray, if
the mode is not added to Favorites.
1. [Fig. 1.2] Press ① [Menu] on the screen.
2. Select a measurement mode to add to Favorites.
3. [Fig. 1.3] Press ② Click [Favorites] on the upper le of the measurement screen to add to Favorites.
4. [Fig. 1.3] Press ② [Favorites] in the corresponding measurement mode to delete a favorite.
Ch.1

18 FastGene®NanoView User Guide Book
Fig. 1.2
The factor designated for each mode is set as the default value. The input mode is addionally provided
so that the user can designate a factor value. It is designed in such a way to enable the measurement of
numerous sample types, besides protein, depending on the tester or experiment environment.
1-3-3 Measurement menu composion
Menu Factor
Nucleic Acid
ds DNA 50
ss DNA 33
RNA 40
Other Input
Protein
Protein 1
BSA 1.49
IgG 0.72
Lysozyme 0.37
Other Input
OD600 OD600 1
1-3 Menu descripon
Ch.1

19
FastGene®NanoView User Guide Book
Ch. 2
Basic Measurements
2-1 Pedestal Basic Use
2-2 Cuvee Basic Use
2-3 Baseline ON / OFF
Ch.2

20 FastGene®NanoView User Guide Book
Warning
2-1 Pedestal Basic Use
Press the [Measure] buon to conduct measurement and display the measured value on the screen.
Open the cell cover aer compleng the measurement. If the sample needs to be collected, extract the
sample carefully using a pipee. If sample collecon is not needed, soly wipe the sampling pedestal and
cover quartz window using a laboratory ssue. For addional washing, drop dislled water onto the
sampling pedestal and cover quartz window, soly wipe it o with a laboratory ssue, and repeat washing
if contaminaon is serious.
Take a 1-2 µl (minimum volume - 0.5 µl) sample using a pipee. Place the pipee used to take the
sample on the sampling pedestal and carefully drop the sample, keeping the shape so that the drop does
not burst. Close the cell cover.
Fig. 2.1
Fig. 2.3Fig. 2.2
If the volume of the sample is less than 1.0 µl or more than 2.0 µl, the sample may not be
posioned correctly on the sampling pedestal and the measured value may not be correct. If
sample concentraon is very low (less than 2 ng/µl, based on dsDNA) or very high (greater than
10,000 ng/µl, dsDNA), the accuracy of the measured value can decrease. Therefore, measure the
sample aer concentraon or diluon according to the range in queson.
Ch.2