NuAire LabGard NU-581-400E Manual

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LabGard® ES HD Energy Saver
Class II, Laminar Flow
Biosafety Cabinet
Models
NU-581-400E/500E/600E
Console
Operation & Maintenance Manual
July, 2019
Revision 4
Series 1
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: [phone]
(400E & 600E ONLY)

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Congratulations!
You have just purchased one of the finest Laminar Flow Biosafety Cabinets available. With proper care, maintenance
(certification), and laboratory procedure, this cabinet will give you years of product and personnel protection from particulate
contaminants as prescribed in NSF/ANSI, EN 12469 and DIN 12980: 2016-10.Please read this manual carefully to familiarize yourself
with proper installation, maintenance and operation of the cabinet. Other reference and guideline materials are available through
the following web sites.
www.hc-sc.gc.ca
www.cdc.gov/od/ohs/
www.absa.org
www.absa-canada.org
www.ebsa.be
www.inspection.gc.ca
www.who.int
www.biosafety.be
www.hse.gov.uk
www.nsf.org
www.cetainternational.org
www.nuaire.com

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ABOUT THIS OPERATION & MAINTENANCE MANUAL
The information contained in this manual is intended to reflect our current production standard configuration model along with
the more frequently purchased options. Any unique additions/modifications/shop drawings are appended in the back flap of this
manual, along with any modifications and/or additions to procedures as outlined in this manual. A copy of the original factory test
report is also appended to this manual. In case this manual and/or test report is lost or misplaced, NuAire retains a copy in our files.
A replacement copy can be obtained by calling or writing NuAire, Inc. stating the model number and serial number and a brief
description of the information desired.

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LabGard® ES HD Energy Saver Class II, Laminar Flow Biosafety Cabinet
Models NU-581-400E/500E/600E Operation & Maintenance Manual
TABLE OF CONTENTS
Section No. 1.............................................................................. General Information
Section No. 2.............................................................................. Models & Features
Section No. 3.............................................................................. Warranty Disclaimer
Section No. 4.............................................................................. Shipments
Section No. 5.............................................................................. Installation Instructions
5.1..................................................................................... Location
5.2..................................................................................... Set-up Instructions
5.3..................................................................................... Testing Methods and Equipment
Section No. 6.............................................................................. Operating the NU-581E
6.1..................................................................................... Biosafety Cabinet Control
6.2..................................................................................... Operating Guidelines
6.3..................................................................................... Operating Sequence
6.4..................................................................................... Ergonomics
6.5..................................................................................... Cleaning Procedures
Section No. 7.............................................................................. General Maintenance
7.1..................................................................................... Decontamination
7.2..................................................................................... Fluorescent Lamp Bulb Replacement
7.3..................................................................................... Primary HEPA Prefilter Replacement
7.4..................................................................................... Secondary HEPA Filter/Motor Replacement
7.5..................................................................................... Sliding Window Replacement & Manual Adjustment
7.6..................................................................................... Airflow Control System Setup & Calibration
7.7..................................................................................... HEPA Filter Leak Tests
7.8..................................................................................... Airflow Smoke Pattern Test
7.9..................................................................................... Cleanliness Classification Test for Pharmacy Application
7.10................................................................................... Main Control Board Description & Replacement
7.11................................................................................... Digital Airflow Sensor Description & Replacement
Section No. 8..................................................................................... Error Messages, Troubleshooting, Option-Diagnostics &
Airflow Sensor Performance Verification
Section No. 9.............................................................................. Remote Contacts
Section No. 10............................................................................ Optional Equipment
10.1................................................................................... Ultraviolet Light
Section No. 11............................................................................ Electrical/Environmental Requirements
Section No. 12............................................................................ Disposal and Recycle
Insert.......................................................................................... Replacement Parts
MANUAL DRAWINGS
ACD-19205................................................................................. NU-581E Airflow Schematic
BCD-19206 ................................................................................. NU-581-400E Specification Drawing
BCD-19207 ................................................................................. NU-581-500E Specification Drawing
BCD-19208 ................................................................................. NU-581-600E Specification Drawing
BCD-19209 ................................................................................. NU-581E Security Drain Valve Installation
BCD-16509 ................................................................................. Auto Decontamination Setup
ASSEMBLY DRAWINGS
BCD-11817 ................................................................................. Control Center & Front Decorative Panel Assembly
BCD-11686 ................................................................................. Sliding Window Assembly & Adjustment
BCD-13923 ................................................................................. NU-581 Manual Base Adjustment
BCD-19210 ................................................................................. NU-581 Window Assembly Hinge Operation
BCD-12281 ................................................................................. Auto Window Switch/Cable Adjustment and Maintenance
BCD-12282 ................................................................................. Auto Window Cable and Window Assembly
ELECTRICAL SCHEMATICS
BCD-19211 ................................................................................. NU-581-400E/500E/600E Electrical Schematic

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LabGard® ES HD Energy Saver
Class II, Laminar Flow
Biosafety Cabinet
Models
NU-581-400E/500E/600E
MANUFACTURED BY:
NuAire, Inc. - Plymouth, Minnesota, U.S.A.
1.0 General Information
1.1 Description
The LABGARD® HD Model NU-581E Laminar Flow Biosafety Cabinet (LFBSC) is a console model, designed for the
preparation of Hazardous Drugs meeting the requirements of DIN 12980: 2016-10.The LABGARD® ES Model NU-581E
utilizes an Energy Saver DC ECM Motor optimally determined forward curved fan for each model size/width to maximize
both energy efficiency and filter loading capacity. The Energy Saver ECM Motor is controlled to airflow setpoints via a solid-
state DC motor controller with digital dual thermistor airflow sensors that provide an automatic compensation (constant
volume control) for both filter loading and line voltage variances.
The Laminar Flow Biosafety Cabinet, (LFBSC) is a product resulting from the development of the "laminar flow" principle
(see Airflow Schematic) and the application of environmental controls as required in the field of biological research or
chemical (hazardous drugs)containment. The LFBSC, when used with proper technique, is an effective primary engineering
control in obtaining the optimum product quality while reducing the potential for exposure of both product and personnel
to airborne biological or particulate chemical (hazardous drugs) agents in low to moderate risk-hazard research and drug
preparation or product operations, as prescribed by the Center for Disease Control (CDC) Atlanta, Georgia.
The NU-581E Console LFBSC meets the requirements of a Class II, since the cabinet conforms to the following requirements:
1. Maintains a minimum inflow velocity of 100 LFPM (.51mps) through the work access opening
2. Has HEPA filtered downflow air that is mixed with the inflow air from a common exhaust plenum
3. Discharges a percentage of air to the outside atmosphere after HEPA filtration
4. Has all biologically contaminated ducts and plenums under negative pressure or surrounded by negative pressure
Cabinets used for work with minute quantities of non-flammable or explosive volatile toxic chemicals and tracer amounts of
radionuclides required as an adjunct to microbiological studies must be exhausted through properly functioning exhaust
canopies.

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1.2 Safety Instructions
These safety instructions describe the safety features of the LABGARD® HD Model NU-581E LFBSC.
The safety cabinet has been manufactured using the latest technological developments and has been thoroughly tested
before delivery. However, the cabinet may present potential hazards if it is not installed and used as instructed for its
intended purpose or outside of operating parameters. Therefore, the following procedures must always be observed:
•The safety cabinet must be operated only by trained and authorized personnel.
•For any operation of this cabinet, the operator must prepare clear and concise written instructions for operating
and cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical regulations, in
particular.
owhich decontamination measures are to be applied for the cabinet and accessories
owhich protective measures apply while specific agents are used
owhich measures are to be taken in the case of an accident
•Repairs to the device must be carried out only by trained and authorized expert personnel.
•Keep these operating instructions close to the cabinet so that safety instructions and important information are
always accessible.
•Should you encounter problems that are not detailed adequately in the operating instructions, please contact your
NuAire Representative of NuAire technical Services.
1.3 Explanation of Symbols
CAUTION used without the safety alert symbol indicates a potentially
hazardous situation which, if not avoided, may result in property damage.
Potential electrical hazard, only qualified person to
Note: Used for important information.
Biohazard
Ground, Earth
CAUTION

Chemical Hazard
Flammable Hazard
Hazardous Gases!
Personal Protection Equipment Required.
Lead Free
Hazardous Drug
Safety alert symbol indicates a potentially hazardous situation
which, if not avoided, could result in death of serious injury.
Safety alert symbol indicates a potentially hazardous situation
which, if not avoided, may result in minor or moderate injury.
WARNING
!
CAUTION
!

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2.0 Models & Features
The model NU-581E, LABGARD® ES HD Class II, Laminar Flow Biosafety Cabinet is manufactured in three sizes:
4ft. (1.2m), 5 ft. (1.5m), and 6 ft. (1.8m).

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