Olympus CIX100 User manual

Instructions
Sample Holders
OLYMPUS CIX100 Cleanliness Inspector System
Optical Microscope Accessory English

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Version 5UM_EVIDENT_CIX100_SampleHolders_en_01_08082022
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Contents
1 Sample holders for the OLYMPUS CIX100 Cleanliness Inspector System 4
1.1 Introduction 4
1.2 Intended use 4
1.3 About these instructions 4
1.4 Available sample holders and sample types 5
2 Safety 9
2.1 Symbols and safety instructions 9
3 Setup of OLYMPUS CIX100 Cleanliness Inspector System at time of delivery 12
4 Adding new sample types to your CIX100 system 13
5 Calibrating Shading Correction for a new sample type 15
6 Calibrating White Balance for a new sample type 24
7 Performing an inspection with different sample types with a CIX100 system 33
8 Best Practices for the versatile sample holders for your CIX100 system 36
8.1 Mounting and inspection of filter membranes in the CIX100 system 36
8.2 Mounting and inspection of tape lift samples in the CIX100 system 38
8.3 Mounting and inspection of particle trap samples in the CIX100 system 41
9 Exchanging the stage insert of a CIX100 system for metallurgical applications 45
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OLYMPUS CIX100 Cleanliness Inspector System
1 Sample holders for the OLYMPUS CIX100 Cleanliness
Inspector System
1.1 Introduction
The OLYMPUS CIX100 Cleanliness Inspector System includes a sample holder for
the inspection of particles on filter membranes with a diameter of 47mm. With
optional additional accessories, filter membranes of different diameters (25mm,
47mm and 55mm) with either white or black background, particle traps and tape
lift samples can be analyzed in your CIX100 system. This manual describes how to
integrate each of these optional sample holders and sample types to your CIX100
system for optimal usability.
1.2 Intended use
All sample holders and accessories mentioned in this manual are intended for the
inspection of technical cleanliness. It is mandatory to follow the sample preparation
procedure for each type of sample, which is described according to the
applications and the standard used. Follow the mounting instructions for each
sample type as outlined in this manual. All sample holders are designed for use
under normal environmental conditions. Use under oxidative or acidic
environmental conditions as well as high temperatures should be avoided.
1.3 About these instructions
These instructions are part of the documentation for the OLYMPUS CIX100
Cleanliness Inspector System. Further information about the operation of the
OLYMPUS CIX100 Cleanliness Inspector System can be found in the CIX100 System
Setup Guide and in the CIX ASW software documentation. Read all of the other
instructions for the components of the system carefully.
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Sample Holders
1.4 Available sample holders and sample types
E9701689 HW-CIX-FH-25B
E9701690 HW-CIX-FH-47B
E9701691 HW-CIX-FH-55B
E9701692 HW-CIX-FH-25W
E9701591 HW-CIX-FH-47W
E9701592 HW-CIX-FH-55W
Sample type name in CIX ASW software:
uncovered membrane
(1) clamp
(2) desk (white or black)
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OLYMPUS CIX100 Cleanliness Inspector System
E9701695 HW-CIX-HTL
Sample type name in CIX ASW software: tape lift
(1)metallic frame with marks for tape
widths 50mm and 19mm
(2) black base with white surface
(3) back side marked with dots
E9701696 HW-CIX-HPT
Sample type name in CIX ASW software: particle trap
E9701688 HW-CIX-PT-10
Particle traps
(1)Black base with a green rubber as
sample stabilizer
(1)Open particle trap with sticky
surface
(2) Plastic container
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Sample Holders
The following table gives an initial indication of which sample holder is
recommended for which application in the CIX100 system.
Article
number Article code Short article description Examples of application fields
E9701689 HW-CIX-FH-25B
Holder with black
desk for filter
membranes with
25mm diameter
Holders with black background can be used to
inspect all opaque membranes- for example
cellulose nitrate.
The main advantage of the holders with black
background is that they do not have any coating
making them resistant when aggressive solvents
are used.
E9701690 HW-CIX-FH-47B
Holder with black
desk for filter
membranes with
47mm diameter
E9701691 HW-CIX-FH-55B
Holder with black
desk for filter
membranes with
55mm diameter
E9701692 HW-CIX-FH-25W
Holder with white
desk for filter
membranes with
25mm diameter
Holders with white background can be used to
inspect all opaque membranes.
The white coating can be damaged by
aggressive solvents.
The main advantage of the white background
holders is the inspection of woven membranes
without showing artifacts from the holder when
looking through the mesh.
E9701591 HW-CIX-FH-47W
Holder with white
desk for filter
membranes with
47mm diameter
E9701592 HW-CIX-FH-55W
Holder with white
desk for filter
membranes with
55mm diameter
E9701695 HW-CIX-HTL Holder for tape lift
inspection
Investigation of cleanliness in which the particles
are lifted from the surface with an adhesive tape..
E9701696 HW-CIX-HPT Holder for particle
trap inspection
Environment analysis by monitoring particles
depositing on a particle trap in a defined time.
The particle traps compatible with CIX100 are not
included.
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OLYMPUS CIX100 Cleanliness Inspector System
E9701688 HW-CIX-PT-10 Particle traps
Particle traps can be used to monitor and
collect particle contaminations in the working
environment with a defined exposure time. The
particle traps can be ordered as a box of ten
traps.
E9701694 HW-CIX-SIFP
CIX100 alternative
stage insert (not
compatible with
sample holders listed
above)
This stage insert is a flat plate which can be
mounted to the CIX100 stage. This is useful
when CIX100 is to be used for metallurgy. The
samples can be placed on top of this flat plate.
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Sample Holders
2 Safety
For all samples used in the CIX100 system, make sure to follow all the instructions
concerning size and weight specified in the System Setup Guide. Please read the
CIX100 System Setup Guide carefully before using your CIX100 system or installing
new hardware to your system.
2.1 Symbols and safety instructions
The exclamation mark and the word ATTENTION indicate
situations where irreparable damage to the product can occur if
ignored.
The warning sign and the word CAUTION indicate dangerous
situations that can lead to light injuries if ignored.
ATTENTION
Device damage through overloading the stage
The maximum permitted load of the stage must not exceed 2 kg. Overloading the
stage can hinder the movement of the stage or damage the microscope. Make sure
that the weight of the stage insert (e.g. multi-sample holder), samples and sample
holders (e.g. filter holder) in total does not exceed the maximum permitted weight
of 2 kg.
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OLYMPUS CIX100 Cleanliness Inspector System
ATTENTION
Device damage from oversize or unsuitable samples
Samples that exceed the maximum permitted height can damage the lenses or the
stage. Make sure that the height of the sample doesn't impair the movement of the
objectives. Do not use any samples that prevent the free movement of the
nosepiece and the installed objectives. Pay attention to the different lengths of the
objectives when moving the nosepiece. Make sure that the sample does not
protrude the sides of the microscope stage.
ATTENTION
When the CIX100 stage is moved or the objective is changed, make sure that the
objectives do not touch the sample or sample holder at all. This is particularly
important when a new sample type has been mounted.
ATTENTION
When the stage is moved, either actively by the user or automatically by the system,
make sure that the current sample and sample holder do not impede the
movement of the stage. When the CIX100 software is used or the joystick is used to
control the stage movement, make sure that nothing hinders the movement of the
system.
ATTENTION
When mounting or unmounting a sample, please make sure that the stage is
moved such that the distance between objectives and sample position you are
working on is maximized. Before returning control of your system to the software
again after changing a sample, make sure that the distance between stage Z-
position and range of motion for the objectives is sufficient for all XY-positions of
the stage.
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Sample Holders
ATTENTION
Make sure that the filter desk of the filter holders and the white surface of the
sample holder for tape lift samples are clean and free of dust. Do not touch these
surfaces directly to avoid fat transmission. If necessary, clean the surfaces with
compressed air or with a dust free lens paper and a few drops of alcohol. Please
pay attention to the safety instructions for the substances applied.
CAUTION
Pinching hazard!
When the stage moves, gaps appear. This creates a pinching hazard. Make sure
that you are not within the stage’s range of movement when it is moving. Try never
to put your hands or fingers into any gaps.
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OLYMPUS CIX100 Cleanliness Inspector System
3 Setup of OLYMPUS CIX100 Cleanliness Inspector System at
time of delivery
The CIX100 system is delivered in a state ready for the inspection of dark and
reflective particles on white, matt filter membranes.
The standard filter holder for membranes of 47mm diameter is included and can
be used directly with a filter membrane. Also, all other filter holders where the filter
is not covered can be directly used in your CIX100 system without recalibration. The
sample type is referred to as uncovered membrane. Additional filter holders can be
ordered. Two filter holders can be mounted to your CIX100 system simultaneously.
For convenient use of samples with different reflectivity than a matt white filter
membrane, e.g. made of cellulose nitrate, it is sensible to redo the white balance
calibration. Whether a white balance recalibration is recommended can be seen
when the filter membrane is inspected in the regular inspection workflow. If the
surface of the filter membrane has a bluish cast, the white balance calibration
should be redone on a clean section of the filter membrane.
No other change in the software settings is to be done for the inspection of
uncovered membranes.
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Sample Holders
4 Adding new sample types to your CIX100 system
For adding new sample types (available with CIX ASW software version number
1.5.2 or higher), please make sure to log into CIX ASW software as administrator.
Then you have full access to the system configuration on the start page of your CIX
ASW software.
1. On the start page, click the [Hardware] button.
lThe [Device List] dialog box opens.
2. Click the [OK] button.
lThe [Device Settings] dialog box opens.
3. In the [Device Settings] dialog box, select the [Sample Type] entry.
lOn the right, you find a list of possible samples. [Uncovered
membrane] refers to the standard precalibrated filter membrane
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OLYMPUS CIX100 Cleanliness Inspector System
sample, which is always activated in the CIX100 system.
4. Select the appropriate other sample type or types you want to activate. If
you are unsure, please compare with the list of sample types and holders
you find on page 4 of this manual and click [OK].
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Sample Holders
5 Calibrating Shading Correction for a new sample type
Please make sure to log into the CIX ASW software as administrator. Then you have
full access to the system configuration on the start page of your CIX ASW software.
Insert a clean cellulose membrane to your system with one of the sample holders.
Calibrate the stage limits with this sample as described in the CIX ASW software’s
help.
1. On the start page, click the [Calibration] button.
2. In the [Calibrations] dialog box, select [Shading Correction] and click
[Calibrate].
lIn the following dialog box, you find a list of observation methods. In
your CIX100 system, each observation method represents a list of
software settings optimized for the analysis under specific optical
conditions. Each sample type requires its own observation method. If
your system is equipped with a real color slider, two observation
methods will have to be calibrated for each sample type for optimal
results. The observation method with real color slider is named after
the particle type appended with “in Real Color Mode” and is always
listed below the observation method for the respective particle type
without real color slider.
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OLYMPUS CIX100 Cleanliness Inspector System
lEach observation method is marked with a tick if the shading
correction has been performed already and marked with a yellow
exclamation mark if the shading correction is still missing.
3. Select an observation method which is still undefined. If you start on top,
you will start with the new sample type without real color slider selected.
Click [Next>] to continue.
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Sample Holders
4. Skip the acquisition of the dark current correction image. The dark current
calibration does not need to be repeated here as it was already calibrated
during installation (i.e. keep the check box checked) and click [Next>].
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OLYMPUS CIX100 Cleanliness Inspector System
5. Select all check boxes in the following dialog except [White Balance] and
click [Next >].
lAll objectives your CIX100 system is equipped with are listed in this
dialog and must be calibrated. Standard objectives are 1.25x, 5x and
10x.
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Sample Holders
6. Go to an empty part of the sample and defocus such that no structures are
visible any more on the display area on the left side.
Usually, this can be done by moving the stage a low position of the Z-
range. A Z-movement can either be performed by joystick or by the buttons
in the “Calibration” dialog box. The relative position of the sample in the
calibrated Z-range is displayed on the right side.
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OLYMPUS CIX100 Cleanliness Inspector System
7. Click [Next>] to allow the system to acquire the correction images for all
objectives in your system.
lA green bar indicates the progress. Do not change any settings until
the acquisition has been finished.
8. Follow the software instructions in the dialog box and click [Next >] to
continue with the following objective.
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