Olympus DP70 User manual

This user’s manual is for the OLYMPUS DP70 Microscope Digital Camera in conjunc-
tion with the OLYMPUS DP2-BSW application software. To ensure safety, obtain opti-
mum performance and familiarize yourself fully with the use of this system, we
recommend that you study this manual thoroughly before operating the camera.
Retain this manual in an easily accessible place near a system for future reference.
This publication is printed on 100% recycled paper.
USER’S MANUAL
DP70 &
APPLICATION SOFTWARE
DP2-BSW

Any copyrights relating to this manual shall belong to Olympus Corporation. We at Olympus Corporation
have tried to make the information contained in this manual as accurate and reliable as possible.
Nevertheless, Olympus Corporation disclaims any warranty of any kind, whether expressed or implied, as
to any matter whatsoever relating to this manual, including without limitation the merchantability or fitness
for any particular purpose. Olympus Corporation will from time to time revise the software described in this
manual and reserves the right to make such changes without obligation to notify the purchaser. In no event
shall Olympus Corporation be liable for any indirect, special, incidental, or consequential damages arising
out of purchase or use of this manual or the information contained herein.
No part of this document may be reproduced or transmitted in any form or by any means,
electronic or mechanical, for any purpose, without the prior written permission of
Olympus Corporation.
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registered in various countries.
© Olympus Corporation
All rights reserved
Manual version September, 29th 2006
Printed in Germany

Table of Contents
Important information .......................................................2
About the contents of this manual ..................................................................2
Warning .............................................................................................................2
Maintenance and Storage ................................................................................3
DP70 - System Chart .........................................................4
The installation process ...................................................4
Installation of the DP70 camera with the DP2-BSW soft-
ware ....................................................................................6
Preparing your computer .................................................................................6
Installing the software ......................................................................................8
Starting the DP2-BSW software ....................................................................14
Acquiring your first images ...........................................14
Special camera functions ..............................................................................14
Super Fluorescence Mode (SFL) ..............................................................................15
Supersampling ..........................................................................................................16
Calibrations .....................................................................16
Camera settings ..............................................................16
General .....................................................................................................................17
Color .........................................................................................................................19
Adjustment ................................................................................................................20
Exposure ...................................................................................................................25
Information ................................................................................................................26
Help with problems .........................................................28

Important information
2
Important information
About the contents of this manual
This manual describes the installation of the DP2-BSW image analysis program, the
setting up of the DP70 camera for use with the DP2-BSW image analysis program,
and the way to operate the DP70 camera with the DP2-BSW image analysis
program.
Warning
Warning This manual does not describe the installation of the camera itself. For this you
should use the DP70 Digital Microscope Camera Instruction Manual that was
supplied with your camera.
See also You will find a detailed description of the camera controls, based on a sample
camera, in your image analysis program's manual. You will find a description of
the camera specific functions in this manual, starting on page 14.
Warning Do not disassemble the camera housing! CCD image sensors are easily damaged
by static discharge.
Warning To avoid damage to the cable and the risk of fire, make sure that the camera cables
do not come into contact with the light source or other sources of heat.
Warning In case of a fire toxic gases can be given off!
Warning Arrange the cables in such a way that nobody can stumble or trip over them.
Warning Do not touch cover slip (over the CCD chip) with your hand or have any object come
in contact with glass surface. Should dust stick to the cover slip, blow it off gently with
an air blower. (For dust stuck due to static electricity, ionized air is recommended.)
Warning Do not subject the camera to too much mechanical shock.
Warning If the cable is damaged or severed, you risk an electric shock if you come in direct
contact with it. Shut off the hardware and change the cable immediately!
Warning To prevent the microscope from toppling down, avoid using microscope attachments
that may make the total height of the microscope above 1 m when they are attached.

Important information
3
Operating Environ-
ment
•Do not expose to strong light (sunlight) for long periods.
•High temperatures (> 40°C) and humidity (> 80%) can influence the camera's
capabilities. Avoid storage or usage under such conditions.
Maintenance and Storage
1) To clean the lenses and other glass components, simply blow dirt away using a
commercially available blower and wipe gently using a piece of cleaning paper
(or clean gauze).
If a lens is stained with fingerprints or oil smudges, wipe it with gauze slightly
moistened with commercially available absolute alcohol.
2) Parts other than the glass components should be cleaned by wiping with a clean
cloth. Do not use organic solvents to remove major stains. Use a soft cloth
slightly moistened with a neutral detergent solution.
3) Do not disassemble any part of the camera as this could cause a malfunction or
reduced performance.
Warning Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electric sparks - for
example, electrical equipment that is being switched on or off.
Also remember to always use these chemicals only in a well-ventilated room.

DP70 - System Chart
4
DP70 - System Chart
The DP70 system chart belongs to the camera's scope of supply. Please go in the
DP70 user's manual to Chapter 1: (The System Chart).
The installation process
You have
purchased the
image analysis
program and
camera together
If you have purchased the DP70 camera together with the image analysis program,
you will need to carry out both the hardware installation for the camera and the soft-
ware installation for the image analysis program.
In order to avoid problems, you will have to follow the steps of the procedure in the
order given here.
Install the PCI board.
This step is described in the DP70 user's manual (Chapter 2: Hardware Instal-
lation, page 8).
Install the camera on your microscope.
This step is described in the DP70 user's manual (Chapter 2: Hardware Instal-
lation, page 9).
Connect the camera to your PC with the cable that was supplied with it.
This step is described in the DP70 user's manual (Chapter 2: Hardware Instal-
lation, page 10).
Prepare your computer for the rest of the installation.
This step is described in this manual, beginning on page 6.
Start the DP2-BSW image analysis program.
Install the DP2-BSW image analysis program.
This step is described in this manual, beginning on page 8.

The installation process
5
You have already
installed the DP70
on your system
If you purchased the image analysis program at a later date, you will not need to
install the camera again.
You can operate your camera by turns with DP2-BSW and another software that you
have already installed. Simultaneously running different software packages that work
with the DP70 is, however, not possible.
Prepare your computer for the installation.
This step is described in this manual, beginning on page 6. Start with step 3.
Start the DP2-BSW image analysis program.
Install the DP2-BSW image analysis program.
This step is described in this manual, beginning on page 8.

Installation of the DP70 camera with the DP2-BSW software
6
Installation of the DP70 camera with
the DP2-BSW software
System requirements
See the DP70 Instructions Manual, page 4, for the system requirements.
Preparing your computer
You can also install the DP2-BSW software on your PC when you have already
installed another software package from Olympus Soft Imaging Solutions on it.
Only if the camera
has not been
installed previously
1) Switch on the PC and start the operating system.
You need administrator rights in order to install the software.
The Found New Hardware dialog box will open. The operating system has
detected the new PCI board.
After starting the
computer this dialog box
will be opened.
Only if the camera
has not been
installed previously
2) Click the Cancel button to abort the installation.
The Found New Hardware dialog box will be closed. The correct drviers will
be installed in conjunction with the image analysis psystem.
3) Insert the software protection key (dongle) into a USB port of your PC.

Installation of the DP70 camera with the DP2-BSW software
7
Your DP2-BSW soft-
ware is protected by a
dongle. Insert it into a
USB port of your PC.
The Found New Hardware dialog box will open. The operating system will
then try to install a driver for the dongle.
When you have plugged
in the dongle, this dialog
box will appear.
4) Since the correct dongle driver can only be installed during the installation of the
image analysis program, you will then need to click the Cancel button.
The Found New Hardware dialog box will close. During the installation of
your image analysis program, the correct dongle driver will be automatically
installed.

Installation of the DP70 camera with the DP2-BSW software
8
Installing the software
1) Close any and all application programs.
2) Put the installation CD-ROM into your CD-ROM drive.
The installation program will open automatically - unless you have deacti-
vated the auto run function. If so, start the setup.exe file via Microsoft
Windows Explorer.
Read carefully through
the license agreement
in the License Agree-
ment dialog box.
Select the Yes, I accept
all terms of the License
Agreement option.
3) To make the installation, simply follow the installation wizard's instructions.
Each time you have made the necessary entry, click the Next > button to con-
tinue.

Installation of the DP70 camera with the DP2-BSW software
9
Select the language you
want in the Language
Selection dialog box.
Your image analysis
program's user interface
and online help will be
displayed in the
language you have
selected.
Enter your User Name
and Company Name in
the Customer Informa-
tion dialog box.
Select the Only for me
(user name) option, to
limit access to the
image analysis
program.
Select the Anyone who
uses this computer (all
users) option, to enable
any user of this PC to
work with the image
analysis system.

Installation of the DP70 camera with the DP2-BSW software
10
Select the directory into
which your image anal-
ysis program is to be
installed, in the Choose
Destination Location
dialog box.
Select the manufacturer
of your microscope's
controller in the Manu-
facturer list, and select
the model in the Micro-
scope list.
Should you not have
any of the devices that
are offered, leave these
check boxes empty and
click the Next > button.

Installation of the DP70 camera with the DP2-BSW software
11
Select the entry DP70 in
the Device list of the
Image Source Selection
dialog box.
Select an option for the
user interface's style
during the image acqui-
sition.
The Standard Style
option corresponds to
the user interface
described in the
manual.
The DP-Controller
option is similar to the
DPController software
that came with your
camera.

Installation of the DP70 camera with the DP2-BSW software
12
Using desktop icons
and shortcuts will give
you quick access to
your image analysis
program and the corre-
sponding documenta-
tion.
Now click the Next >
button to start the actual
copying and installation
procedure.

Installation of the DP70 camera with the DP2-BSW software
13
The progress bar in the
Setup Status dialog box
will provide you with
information about the
status of the installation.
When the installation
has been completed,
the InstallShield Wizard
Complete dialog box will
appear.

Acquiring your first images
14
Starting the DP2-BSW software
Start your image analysis program by doubleclicking the corresponding link. If you
did not set up a link when you made the software installation, you can start your
image analysis program via the Windows start menu.
Acquiring your first images
Prerequisites You have successfully installed your system (DP2-BSW software, camera, and
camera driver). You would like to acquire your first images.
1) Start the DP2-BSW software.
You will automatically be in the Acquisition layout. This means that all of the
software controls are optimally positioned on your monitor for acquiring im-
ages.
On the right-hand edge of your screen you will find the Camera Control tool
window. There, the Live and Snap buttons are active. You can now acquire
images and make changes in the camera settings.
Live
Click the Live button in the Camera Control tool window or use the Acquire > Live
command.
The acquired image is shown live in the document window.
Use the live-mode for focussing or when arranging the camera or the sample.
Snap
Click the Snap button in the Camera Control tool window or use the Acquire > Snap
command.
Use the Snap mode, to stop the live-image or to acquire a snapshot.
You can edit or save the image you have acquired. The way to make image acquisi-
tions is described in detail in your DP2-BSW software manual.
Special camera functions
In the following you will find an explanation of the special controls in your DP2-BSW's
Camera Control tool window. You will only find these controls when you work with
the DP70. All of the other functions you will find described in detail in your DP2-BSW
software's manual.
See also You will find a detailed description of the camera controls, based on a sample
camera, in your image analysis program's manual. You will find a description of
the camera specific functions in this manual, starting on page 14.

Acquiring your first images
15
The DP2-BSW
program's Camera
Control tool window.
Only the DP70 specific
functions SFL (Super
Fluorescence Mode)
and Supersampling will
be described here.
Super Fluorescence Mode (SFL)
The automatic determination of the exposure time will, in normal use, be set so that
a small proportion of the image's pixels will be overexposed. This makes an optimal
presentation of the image possible in the brightfield microscopy. With fluorescence
acquisitions, this setting can endanger the image quality, since it is precisely the
bright image areas that are to be reproduced. In this case, you should select the SFL
check box. In the SFL mode, image pixels will be prevented from ending up in the
overflow.
Super Fluorescence Mode (SFL), see
page 15
Supersampling, see page 16

Calibrations
16
Supersampling
Your DP70 camera can reach a higher resolution with separate images than the CCD
chip provides for. This acquisition procedure, called supersampling, is not available
for the live mode.
An acquisition using supersampling requires a relatively large amount of time. The
task bar will be displayed in the status bar.
While the acquisition is taking place you can't open any further camera functions. To
abort the acquisition, click the Cancel button.
Calibrations
To reach the highest possible acquisition quality, the system made up of microscope
and camera, must be correctly calibrated. You will find an explanation of how to do
this in the DP2-BSW manual.
SHADING CORRECTION You will find a description of the online shading correction, and the way to use it, in
your manual on page 21.
Camera settings
To open the Camera Settings dialog box, click the Settings button located in the
Camera Control tool window. In this dialog box, as well as making settings that are
general for cameras, you can also make a number of settings that are specifically for
your DP70 camera.

Camera settings
17
In the Camera Settings
dialog box, click the
Olympus DP70 entry, to
open the pages that are
specially laid-out for
your camera.
General
Use this dialog box in the Camera Settings to make a variety of general camera
settings. You can, for example, mirror the camera image, or select a pseudo color
table.

Camera settings
18
Mirror Select the Horizontal check box to mirror the image horizontally during acquisition.
Select the Vertical check box to mirror the image vertically during acquisition.
The two forms of mirroring can be combined with each other.
Use the mirroring function to match the presentation of the image on your monitor
with that in the eyepiece.
Image Type Select the default option "24 bit RGB Color", to acquire images of the "24-bit true-
color" type.
Select the default option "48 bit RGB Color", to acquire images of the "48-bit true-
color" type. Not every other application program will be able to open such images.
Select the "8 bit Gray Scale" option to acquire images of the "8-bit gray-value" type.
Select the "16 bit Gray Scale" option to acquire images of the "16-bit gray-value"
type. Not every other application program will be able to open such images.
Custom gray scale Select the Custom gray scale check box, to not acquire gray-value images over the
whole visible spectrum. When you have selected the Custom gray scale check box,
three more check boxes, R(red), G(green) and B(blue), will be available to you.
These check boxes stand for the camera's separate color channels. When you delete
one of these check boxes, the corresponding color channel will no longer contribute
to the gray-value image.
Use the possibility to fade out color channels, respectively use only one color
channel, for the acquisition of a gray-value image, to match the camera's spectral
sensitivity with fluorescence acquisitions.
Pseudo Color You can use pseudo color tables to have images displayed in color on your monitor.