Olympus LC35 User manual

Instruction manual
OLYMPUS LC35
Digital Microscope Camera
Optical Microscope Accessory English

Contents
1 Safety 4
1.1 Intended use 4
1.2 Avoiding personal injury and device damage 4
1.3 Explanation of the symbols used 6
2 The OLYMPUS LC35 camera 7
2.1 Scope of supply 7
2.2 System diagram 8
2.3 LED status indication 9
2.4 Specifications 10
2.5 Operating and storage conditions 11
3 System environment 12
3.1 Microscope and camera adapter 12
3.2 Recommended computer and monitor configuration 13
3.3 Restrictions on use 13
4 Assembly 15
4.1 Attaching the camera 15
4.2 Connecting the USB camera cable 16
4.3 Installing the software 17
5 Acquiring images 18
6 Cleaning the camera 19
7 Troubleshooting 20
7.1 Possible problems 20
7.2 Contacting Customer Service 24
8 Declarations of conformity and notes on disposal 25
AdressSticker_08042022 29

About the camera
The OLYMPUS LC35 is a CMOS color camera with a high frame rate.
It was developed for acquisitions using light microscopy. The camera
can be connected to all common types of light microscopes using a
C-mount.
About this instruction manual
This instruction manual is for the OLYMPUS LC35 microscope digital
camera.
Information about the operation of the microscope, computer or
software can be found in the instruction manuals for the
corresponding device or software.
Trademark
Microsoft and Windows are registered trademarks of Microsoft
Corporation, USA. All other brand names and product names
mentioned in this manual are registered trademarks or trademarks
of their respective owners.
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1 Safety
The camera has been developed and produced in conformity with
recognized safety regulations. However, it is not possible to entirely
rule out possible hazards for the user, damage to the camera or
microscope, or impairments of the camera's functionality. For this
reason, always read and follow the general safety instructions and
warnings before and during installation.
1.1 Intended use
This camera is intended to be used for the acquisition of digital
images, but not for medical diagnostic purposes.
1.2 Avoiding personal injury and device damage
WARNING - Avoiding personal injury
Electric shock due to damaged cable
When a cable is frayed or damaged, a person touching it risks
receiving an electric shock. Shut off the hardware and replace the
cable immediately.
Poisoning due to toxic gases
In the event of a fire, the material of the camera can give off toxic
gases. When these are inhaled, it can cause irritation or damage to
the respiratory tract. Wear respiratory protection when fighting the
fire.
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CAUTION - Avoiding personal injury
Tripping hazard
Cables laid across a room can be a dangerous tripping hazard. This
can cause injuries to people and damage to equipment. Wherever
possible, lay cables along the wall or behind furniture. Fix and label
cables that run across the room.
ATTENTION - Avoiding device damage
Never open the camera
The camera's CMOS chip is extremely sensitive. Electrostatic
discharge can permanently damage the camera. Therefore, never
open the camera housing.
Use only the supplied USB camera cable
Operate the camera only with the supplied USB camera cable. Only
then will the functionality of the camera and compliance with the
EMC basic standards be ensured.
Don't touch the protective glass
The protective glass over the CMOS chip is extremely sensitive and
must not come into contact with bare hands or any other objects,
otherwise the acquired images will be impaired by fingerprints or
scratches.
Avoid vibration shocks
Mechanical shocks and strong vibrations can damage the camera.
Don't drop the camera during assembly. Make sure the camera is
securely mounted on the microscope. When the camera is not in
use, store it in a safe place.
1 Safety
5

Comply with operating and storage conditions
Exposure to high temperatures or humidity can damage component
parts of the camera. Always observe the operating and storage
conditions (see page 11).
1.3 Explanation of the symbols used
WARNING
Indicates a potentially hazardous situation which, if not avoided, may
result in major injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may
result in minor or moderate injury.
ATTENTION
Indicates a situation which, if not avoided, may damage the
equipment or other property.
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2 The OLYMPUS LC35 camera
2.1 Scope of supply
Camera
USB camera cable
Safety and warning
information
A printed copy of the safety and warning information are supplied
with the camera.
2 The OLYMPUS LC35 camera
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The complete instruction manual is available for download. You can
download the manual from the following web pages
https://www.olympus-lifescience.com/support/instruction-manual-
downloads/
https://www.olympus-ims.com/user-manuals/
2.2 System diagram
To use the OLYMPUS LC35 with a light microscope, you need
suitable software and a computer as well as a monitor. For the
recommended computer and monitor configuration, refer to page
13.
You can use the following Olympus software applications:
Software application Minimum version
cellSens* Life science image analysis
software 3.2
OLYMPUSStream* Image analysis software 2.5
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Software application Minimum version
PRECiV* Image and measurement
software 1.1
DP2-TWAIN** Free image acquisition software 10.2
LCmicro** Free image acquisition software 2.3
* Perform the available service updates.
** This software is available only as a download from the following
web pages.
https://www.olympus-lifescience.com/support/downloads
https://www.olympus-ims.com/service-and-support/downloads
2.3 LED status indication
There is an LED on the camera which indicates the following
statuses.
LED Status
The LED flashes green. The camera is being initialized.
The LED lights up
continuously green.
The camera is successfully connected to the
USB 3.1 port.
The LED flashes red/orange. There is a camera error. Contact Customer
Service (see page 24).
The LED lights up orange.
The camera is not connected to the USB 3.1
port. If this is the case, you will receive a
corresponding error message.
2 The OLYMPUS LC35 camera
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2.4 Specifications
The OLYMPUS LC35 is a CMOS color camera with a high frame rate.
It was developed for acquisitions using light microscopy. The camera
can be connected to all common types of light microscopes using a
C-mount.
Chip type CMOS color sensor
Chip size 1/2.5 Inch
Recording area 5.702 mm x 4.277 mm
7.13 mm diagonal
Maximum resolution 2160 x 1620 pixels
3.5 million pixels
Camera port of the microscope C-Mount
Input / output On the camera:USB 3.1 type C
On the computer: USB 3.1 type A
Resolution Frame rate* Exposure
time
2160 x 1620 Full resolution 19 fps
25 µs - 1534
ms
1296 x 972 Fast mode 40 fps
1920 x 1080 Full HD 25 fps
* The maximum achievable frame rate is listed. The frame rate depends,
among other things, on the computer being used.
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2.5 Operating and storage conditions
Always meet the operating and storage conditions.
Operating environment
Ambient temperature +5 °C - +40°C
Relative humidity 5% - 85% (without condensation)
Altitude Up to 2000 m
Storage conditions
Ambient temperature -20°C - +60°C
Relative humidity 10% - 90%
Camera storage
When not using the camera, attach the cover to the C-mount and
store the camera in a safe place.
2 The OLYMPUS LC35 camera
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3 System environment
3.1 Microscope and camera adapter
Supported microscopes
Upright microscopes
CX23 / CX33 / CX43 / BX43 / BX46 / BX53/ BX53M
Inverted microscopes
CKX53
Stereo microscopes
SZX7 / SZX10 / SZX16 / SZ61TR
Camera adapter connected to OLYMPUS LC35
U-TV1XC / U-TV1X+U-CMAD3 O
U-TV0.63XC O
U-TV0.5XC-3 O
U-TV0.35XC-2 (-)*
0.5XC (integrated in SZ61TR) O
O Compatible
(-)* Limited compatible: Depending on the microscope used, this
camera adapter can lead to a point-like brightening in the center
of the image and shadowing at the edge of the image
You can find more information in chapter "Restrictions on use" on
the facing page.
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3.2 Recommended computer and monitor
configuration
When you connect the OLYMPUS LC35 to a computer, the following
system requirements must be met.
CPU Intel® Core i5, Intel® Core i7, Intel® Core i9
Intel® Xeon (or equivalent)
CPU cores Recommendation: 4 or more physical CPU
cores
RAM Recommendation: 8 GB or more (dual
channel)
Monitor Resolution 1920 x 1080 or higher
Communication port USB 3.1 type A
Removable media drive Optical drive (double-layer capable)
Computer input device
Recommendation: 3-button mouse with
wheel.
Keyboard
Operating system Microsoft® Windows® 10 (64 Bit)
3.3 Restrictions on use
OLYMPUS LC35 With other microscopes
The camera can be used in combination with many available
microscopes. However, the performance of the camera can only be
guaranteed for the microscopes listed in the"Microscope and
camera adapter" chapter on page 12.
The camera’s performance in combination with other microscopes
has not been tested.
3 System environment
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Brightness fluctuations in the camera image
With very high illumination intensities and short exposure times at
the same time, the following phenomena can occur:
nFlickering in the image displayed
nUneven exposure
If possible, reduce the illumination light level or use an attenuation
filter to increase the exposure time to 20 ms or longer.
Clouding at the edges of the field of view caused by an intermediate
tube that is too long
If the intermediate tube is too long (see following example), clouding
can occur at the edges of the field of view.
Examples of an intermediate tube that is too long:
nTwo or more intermediate adapters are being used
together.
nBX3 series: The vertical illuminator and the intermediate
adapter are being used together.
Flare when the aperture stop is small
Spot flare can occur when the aperture stop is closed. This flare can
be decreased by opening the aperture stop.
No support of simultaneous image acquisition using several
cameras
You can mount several OLYMPUS LC35 cameras to one microscope.
However, it isn’t possible to simultaneously operate several cameras
with one computer.
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4 Assembly
4.1 Attaching the camera
1. Remove the cap of the C-mount port (a).
2. Screw the C-mount camera adapter (b) into the C-mount
thread (c) at the bottom of the camera head.
3. Attach the C-mount camera adapter to the camera port of
the microscope.
Checking the camera’s orientation
If the camera is not attached in the correct orientation, the camera
image does not match the image visible through the eyepieces.
1. After attaching the camera, compare the eyepiece image
with the live image on the monitor.
2. Rotate the camera adapter so that the alignment of these
images matches.
4 Assembly
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4.2 Connecting the USB camera cable
ATTENTION
Use only the supplied USB camera cable
Operate the camera only with the supplied USB
camera cable. Only then will the functionality of the
camera and compliance with the EMC basic standards
be ensured.
1. Connect the USB type C plug (a) to the camera’s USB type C
socket (b).
2. Connect the USB camera cable's USB type A plug (c) to the
USB 3.1 port (d) of the computer.
Depending on the computer, the USB 3.1 port is either
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colored blue or labeled "SS".
The camera can’t be operated using a USB 2.0 port.
4.3 Installing the software
cellSens, OLYMPUS Stream, PRECiV
Read the installation manual before installing the software.
TWAIN driver DP2-TWAIN, LCmicro
This software is available only as a download from the Olympus
Internet site.
https://www.olympus-lifescience.com/support/downloads
https://www.olympus-ims.com/service-and-support/downloads
4 Assembly
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5 Acquiring images
You can find detailed instructions in the instruction manual or in the
Help of the software you are using.
Example of the acquisition process
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6 Cleaning the camera
When correctly handled, the camera has a long life span and is to a
large extent maintenance-free.
Cleaning the protective glass
To clean the camera's protective glass, use a standard rubber air
blower to remove the dust and clean it gently with a clean lens cloth.
If there are fingerprints or oil smudges on the protective glass,
carefully clean it with a clean lens cloth moistened with pure alcohol.
Cleaning the housing
Parts other than the glass components should be cleaned with a
clean cloth. Do not use organic solvents to remove major stains. Use
a soft cloth moistened with a neutral detergent solution.
ATTENTION
Never open the camera
The camera's CMOS chip is extremely sensitive.
Electrostatic discharge can permanently damage the
camera. Therefore, never open the camera housing.
6 Cleaning the camera
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7 Troubleshooting
7.1 Possible problems
Problem: The camera is not working
Possible cause Remedy
The USB camera cable is not
connected properly.
Connect the USB camera cable properly.
When the USB camera cable is
connected properly, the LED lights up
continuously green before the software
starts.
The USB camera cable is
connected to a USB 2.0 port. Connect the camera to an USB 3.1 port.
Problem: Live image is not displayed
Possible cause Remedy
The illumination of the
microscope is not turned on. Switch on the microscope illumination.
The light path selector of the
microscope is not set to the
camera.
Set the light path selector of the
microscope to the camera output.
The illumination intensity of the
microscope is not adjusted
properly.
Set the illumination intensity of the
microscope correctly.
Live image is very dark Increase the exposure time.
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