Omegawave OMEGAZONE User manual

191030
Laser Speckle Blood Flow Imager
OMEGAZONE
OZ-2 mini
User’s MANUAL
( LSI : ver. U4.2, LIA : ver4.3)

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TABLE OF CONTENTS
Page
■ CAUTION 2
■ SUMMARY 3
■ HARDWARE 4
1. Parts name and the function
2. Setup of the stand
4. Lens adjustment
5. Polarization adjustment
6. Block Diagram
■ LSI software 13
1. Controls and display
2. Project Configuration File
3. LSI Front Panel
4. LSI operation
■ LIA software 26
1. Controls and display
2. Analysis Configuration File
4. LIA Front Panel
5. LIA operation
■ Handling in use 42
■ Appendix 44
1. Lens, WD and measurement area(Working Distance)
2. Time for saving data and capacity
3. User name and organization change
4. License certification of OS
■ Specification 46
■ Guarantee 48

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CAUTION
1. Do not turn on the power switch of the power supply for the laser
unit and CCD camera before connecting the cables of the laser unit
and the CCD camera.
2. Do not remove or insert the removable HDD in the state that the
computer is switched on. Also, do not lock or unlock the key of the
removable HDD in the state that the computer is switched on. The
HDD and data may be destroyed.
3. Check that there is enough capacity in the HDD for storing data
before measuring. Data cannot be stored if there is not enough
capacity.
4. Use gloves when the lens and CCD camera are installed or
uninstalled. It is hard to get oil sticks on them.
5. Do not see the laser light directly or through a lens or an optical
instrument.
＿＿＿＿＿＿＿＿＿＿＿＿＿＿
This manual is changed without a preliminary notice.
There is a copyright of this manual in OMEGAWAVE, INC.
A reproduction without permission is prohibited in any kind of form.
Microsoft and Windows are registered trademarks of Microsoft Corporation.
Intel and Pentium are registered trademarks of Intel Corporation.
OMEGAWAVE, INC. has the copyright of LSI and LIA software.

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SUMMARY
The laser speckle blood flow imager, OMEGAZONE, is the instrument to obtain
the colored blood flow image of living tissue non-invasively and continuously.
OMEGAZONE shows more than 1 images/sec with high resolution (639 - 480),
or 15 images/sec with low resolution (212 - 160).
Every point is captured by the pixel of the CCD camera simultaneously,
therefore, there is no time lug between the all points and the each blood flow
value can be compared.
This OMEGAZONE OZ-2 mini consists of the laser unit, the CCD camera, the
lens + hybrid filter, the computer-based image processor and the stand. The AC
power is just needed for the computer-based image processor, and it is easy to
handle and operate.
OZ-2 mini is very compact and it can be used in the small space in a laboratory.
The lens can be changed to adapt the measurement area. The measurement
area is about from 7 mm x 9 mm to 60 mm x 80 mm, and it is suitable for
cerebral blood flow measurement and limb blood flow measurement for small
animals.
OZ-2 mini includes two kinds of software. One is for measurement, LSI, and the
other is for analysis, LIA. Separating the software makes the operation easier.
This instrument was developed based on the study of Dr. Kevin Forrester in
University of Calgary.

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1.Computer-based
Image Processor
HARDWARE
1. Parts name and Function
1) Computer-based imager processor
This computer-based imager processor shows tissue blood flow images
calculated by the installed software. The images captured by the CCD camera
are processed to obtain the blood flow images. The operating system (the OS) is
Windows 7. Turn the power switch (I) on the keyboard after all cables are
connected.
The blood flow software and other application software are installed in the C
drive. Save the data in the E drive. Initial setting shows the E drive for saving.
One hard disk drive is installed in this computer-based image processor, but it is
separated to the C and E.
1.Computer-based
Image Processor
4.Laser Unit
3. CCD camera
+ Lens
2. Power supply
5. Pole Stand
6. USB key

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2) Power supply
The power supply contains the laser drive circuit, and the intermediate
connector for the CCD camera. The power of this power supply is from USB of
the computer-based image processor, and it does not have the power switch
itself.
Turn on the laser switch after the laser unit was connected. Connection after the
power being ON may destroy the laser diodes.
Front Rear
The Laser SW is for ON/OFF of the laser light. The 6-pin laser cable is
connected between the Laser unit and the Connector for laser unit.
The USB connectors have IN and OUT. Connect the CCD camera cable from
the CCD camera to the IN, and connect the male-male USB cable from the OUT
to the computer-based image processor. The IN and OUT is connected inside
the power supply, and the CCD camera is not damaged by misconnection.
Connect the USB power cable to the Power connector.
USB power cable 6-pin laser cable CCD camera cable
Connector for
Laser unit
USB connectors for
CCD camera and the
computer
Power connector
Laser SW

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3) CCD camera and Lens
The images are captured with the CCD
camera. The camera is held on the hold
plate.
The hybrid filter is attached on the lens.
This is the specialized filter combined of
the polarizer and the color filter not to
detect the direct reflection from tissue
surface and the light from outside.
The filter has the direction of
polarization, and it is shown as the white
dot.
Make the white dot showing the front.
50 mm lens
25 mm lens
The standard iris for 50 mm is “ 4 “, and “ 5.6 “ for 25 mm lens. The iris of 5.6 is
the white dot between 4 and 8.
Two space adjusters (spacer) are included. One is the 10 mm and the other is
the 5 mm. Insert the spacer between the CCD camera and the lens. The
measuring area matrix by the set of the lens and the spacer is shown in
Appendix.
10 mm 5 mm
Hold plate
CCD
camera
Iris
・
White dot
Hybrid filter

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4) Laser unit
The laser diode (wavelength : 780 nm) is installed in the unit.
The shutter is attached on the bottom.
Pull the shutter knob to open.
※ Shut the shutter when the
measurement is over to not to
collect dust on the lens inside.
5) Pole stand
The Hold plate with the CCD camera, the lens and the laser unit is fixed.
Adjust the working distance (WD), distance between the lens and the tissue
under study, according to the size of the tissue.
Use the SUS board under the Magnet base and make the magnet ON so that
the Stand does not fall down.
Spread the black sponge sheet under the animal when the tissue does not
occupy the display to obtain clear blood flow images.
6) USB key
This key allows the blood flow imager software, LSI and LIA, to work.
Shutter
knob
Magnet base
Cramp
2 Poles
Stopper
Hold plate

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2. Set up of the Pole stand
The Pole stand is composed of the magnet base, the cramp, the stopper and 2
poles.
(1) Insert the longer pole to the
magnet base. Fix the pole by the
screw on the upper position.
(2) Put the cramp through the longer
pole. Insert the shorter pole to the
cramp.
(3) Attach the Hold plate to the
shorter pole by screwing.

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(4) Install the Laser unit on the Hold
plate by the exclusive screw.
(5) Turning the knurling part can be used
for fine adjustment of the working distance.
The range is 10 mm.
(6) Connect the CCD camera cable and
the laser cable to the CCD camera and the
Laser unit.
アーム
Exclusive screw

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3. Wiring
1) Connection for the
Computer-based image
processor
(1) Connect the AC power cable to
the rear side.
(2) Connect the USB key to the
USB connector on the right
side.
(3) Connect the USB cable from
the CCD camera to the USB
connector on the left side.
(4) Connect the USB power cable
for the power supply to the USB
connector on the left side.
AC power
USB key

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4. Adjustment of Lens
The mono-focal lens has iris and a focus adjustment function.
(1) Select the lens and the space adjuster for the desired measurement area,
and decide the WD (working distance). Set the Hold plate for the WD by
using the Cramp.
(2) Set the iris to “ 4 “ for MF50 lens, and “5.6” (white dot between 4 and 8), for
MF25 . The iris number may be different for the different lens.
(3) Adjust the pint to get clear image.
5. Adjustment of polarization
Not to detect the reflection from tissue surface, Laser Unit and the hybrid filter
of lens have the polarization function. The each white mark can be used for
setting this function. To make the setting exact, the reflector can be used. Turn
the ring of the hybrid filter at the point the reflector is shown as blackest.
Iris
Set the iris at the white line.
Pint
Reflector

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Computer-based imageprocessor
CCDcamera
Lens
Hybrid Filter
Laserunit
780nmLaser
drivecircuit
AC100-220V ACAdaptor
780nm
Laserdiode
Inlet
6PConnector
Shutter
5V
USB
Lens
USBConnector
6. Block Diagram

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LSI Software
（LSI ver.U4.2）
1. Summary
The LSI software is for measuring and saving blood flow images.
This software consists of two views.
■Project Configuration File
■LSI Front Panel
2. Project Configuration File
The display is shown after the LSI worked.
1) Working Directory and File name
The display shows the directory where images will be stored.
The file name is the name that will be the prefix for all image files to be saved.
During the image capture process, the program saves image files under names
composed of the file name, the blood flow mode, the image type and the image
number representing its order in the captured sequence.
For example, the folder name of “ Test “ is made in the volume E in the hard
disk drive. There is one hard disk drive in the computer-based image processor,
but it is separated and assigned as the C and E.

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The folder " Test " is opened after clicking " OK ".
Assign the file name in the box. Without assigning a specific name, “Image” will
be the file name automatically.
Do not use “BF” for the original file name because it is recognized as the “Blood
Flow Image” file.
Actual fine name is created as” Image HR BF R3T~3500No001”.
HR : Kind of mode
BF : Kind of image
R3 : Round
T~3500 : Time
No001 : Number o the image

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The specific file name that a user can assign is the place for “Image”. Do not
change the name after the specific name. The software cannot recognize as the
sequence data images when the file name after the specific name are changed.
When the file specific names have to be changed, change the all-specific
names of the sequence data images.
" Project Configuration File " is displayed after clocking " OK ".
Project Configuration File
2) Information
The information about the measurement can be filled in this file.
" New experiment " is entered in this case.
3) Date and Time
The system date and time are shown here.
4) Total Disk Size
Displays, in bytes, the total size of the disk containing the working directory.
5) Free Disk Space
Displays, in bytes, the free space remaining on the disk containing the working
directory. (When the total free disk space is below 30 Mega-bytes the user is
warned.)
6) Used Disk Space
Displays, in bytes, the used space of the disk containing the working directory.

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7) Measurement
Press the OK button to go to the LSI Front Panel or the Cancel button to exit
the LSI program.

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18
15
6
5
12
7
8
11
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3. LSI Front Panel
LSI Front Panel
Base display
Example of measurement
3
4
2
1
9
13
10
17
16
19

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1) Video Controls
These are controls to display real and real-time images of blood flow images.
Click the switch to show the images. Click again to stop showing the images.
In the Real Video mode, only real images are displayed at 30 frames per
second.
In the Real Video mode, the user can refer to the Real Image Display window
to focus and adjust the camera lens, and to align the tissue and appropriate
region of interest. The gain of the brightness can be adjusted by using the slide
bar controller next to the display. When the brightness is saturated, the controller
shows the red signal. Lower the gain not to show the red signal.
In the BF Video mode, the blood flow and real images are displayed
simultaneously. The blood flow video rate depends on the blood flow mode.
The user can set up blood flow analysis region in the Blood Flow Image
Display window using the Tool Palette and observing the Mean Blood Flow
Plot, Blood Flow Histogram and Mean Blood Flow Value outputs as they are
displayed in real-time.
The color variation is assigned by the color bar next to the Blood Flow Image
Display. There are two kinds of color bars. One is Standard, and the other is
Enhanced. Choose the proper one for the blood flow images. The color bar can
be selected from the Color Bar in the Tool on the Top menu.
2) Blood Flow Mode
There are three modes for the LSI.
The time for saving the image data depends on the color distribution of the
blood flow image and the vacancy of the hard disk.
(1) HR : High Resolution
This mode provides blood flow images with the highest resolution. It is done
by capturing a sequence of 20 real images and later analyzing the speckle
fluctuations at each pixel in the sequence of 20 images.
Resolution : 639 - 480
Name of the saved file : HRBF No
Maximum display speed : about 0.7 sec / image
(2) HS : High Speed
This mode provides blood flow image capture with the highest speed. It
analyzes the spatial fluctuations of speckle in a single real image.
Resolution : 212 - 160
Name of the saved file : HSBF No
Maximum display speed : about 0.07 sec / image
(3) HS AVG : High Speed AVG
This mode provides an average of “ X “ High Speed images, where “ X “ is

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an integer value that can be controlled by adjusting the “ Multi “ value in the
HS AVG box.
Resolution : 212 - 160
Name of the saved file : HSABF No
3) Blood Flow Capture Controls and Parameters
The function allows image(s) to be captured and saved. Both real and blood
flow images are saved to the selected directory entered in the folder assigned at
Working Directory and File name.
Capturing the Blood flow and Real images is activated automatically by pressing
the Auto Save during measurement. The number of images is beforehand set
by the N of Save.
Capturing the Blood flow and Real images is activated manually by pressing the
Manual Save during measurement. Press the button to stop the capturing
images.
The interval time between the images can be set by the Interval function.
The number of the saved images is shown in the box of Current F, and the total
number of the saved images is shown in the box of Saved F.
The number of the Run is renewed by each run of BF Video.
4) File Management Controls
There are functions for loading images and saving images.
Pressing the Load Image button allows to showing the saved images. Select the
image files from Blood Flow Image or Real Image folders in the folder saved
images are stored.
Pressing the Save Image button allows to saving one image displayed on the
screen during measurement.
5) MAX, MIN
This function is to change the color effects by setting the maximum and blood
flow value of the color bar.
(1) MAX : Sets the maximum value in the box.
(2) MIN: Sets the minimum value in the box.
6) Information Display
The information entered in the Project Configuration File is shown here.