Omegawave OMEGAZONE User manual

191030
Laser Speckle Blood Flow Imager
OMEGAZONE
OZ-2 mini
User’s MANUAL
( LSI : ver. U4.2, LIA : ver4.3)

1
TABLE OF CONTENTS
Page
■ CAUTION 2
■ SUMMARY 3
■ HARDWARE 4
1. Parts name and the function
2. Setup of the stand
4. Lens adjustment
5. Polarization adjustment
6. Block Diagram
■ LSI software 13
1. Controls and display
2. Project Configuration File
3. LSI Front Panel
4. LSI operation
■ LIA software 26
1. Controls and display
2. Analysis Configuration File
4. LIA Front Panel
5. LIA operation
■ Handling in use 42
■ Appendix 44
1. Lens, WD and measurement area(Working Distance)
2. Time for saving data and capacity
3. User name and organization change
4. License certification of OS
■ Specification 46
■ Guarantee 48

2
CAUTION
1. Do not turn on the power switch of the power supply for the laser
unit and CCD camera before connecting the cables of the laser unit
and the CCD camera.
2. Do not remove or insert the removable HDD in the state that the
computer is switched on. Also, do not lock or unlock the key of the
removable HDD in the state that the computer is switched on. The
HDD and data may be destroyed.
3. Check that there is enough capacity in the HDD for storing data
before measuring. Data cannot be stored if there is not enough
capacity.
4. Use gloves when the lens and CCD camera are installed or
uninstalled. It is hard to get oil sticks on them.
5. Do not see the laser light directly or through a lens or an optical
instrument.
＿＿＿＿＿＿＿＿＿＿＿＿＿＿
This manual is changed without a preliminary notice.
There is a copyright of this manual in OMEGAWAVE, INC.
A reproduction without permission is prohibited in any kind of form.
Microsoft and Windows are registered trademarks of Microsoft Corporation.
Intel and Pentium are registered trademarks of Intel Corporation.
OMEGAWAVE, INC. has the copyright of LSI and LIA software.

3
SUMMARY
The laser speckle blood flow imager, OMEGAZONE, is the instrument to obtain
the colored blood flow image of living tissue non-invasively and continuously.
OMEGAZONE shows more than 1 images/sec with high resolution (639 - 480),
or 15 images/sec with low resolution (212 - 160).
Every point is captured by the pixel of the CCD camera simultaneously,
therefore, there is no time lug between the all points and the each blood flow
value can be compared.
This OMEGAZONE OZ-2 mini consists of the laser unit, the CCD camera, the
lens + hybrid filter, the computer-based image processor and the stand. The AC
power is just needed for the computer-based image processor, and it is easy to
handle and operate.
OZ-2 mini is very compact and it can be used in the small space in a laboratory.
The lens can be changed to adapt the measurement area. The measurement
area is about from 7 mm x 9 mm to 60 mm x 80 mm, and it is suitable for
cerebral blood flow measurement and limb blood flow measurement for small
animals.
OZ-2 mini includes two kinds of software. One is for measurement, LSI, and the
other is for analysis, LIA. Separating the software makes the operation easier.
This instrument was developed based on the study of Dr. Kevin Forrester in
University of Calgary.

4
1.Computer-based
Image Processor
HARDWARE
1. Parts name and Function
1) Computer-based imager processor
This computer-based imager processor shows tissue blood flow images
calculated by the installed software. The images captured by the CCD camera
are processed to obtain the blood flow images. The operating system (the OS) is
Windows 7. Turn the power switch (I) on the keyboard after all cables are
connected.
The blood flow software and other application software are installed in the C
drive. Save the data in the E drive. Initial setting shows the E drive for saving.
One hard disk drive is installed in this computer-based image processor, but it is
separated to the C and E.
1.Computer-based
Image Processor
4.Laser Unit
3. CCD camera
+ Lens
2. Power supply
5. Pole Stand
6. USB key

5
2) Power supply
The power supply contains the laser drive circuit, and the intermediate
connector for the CCD camera. The power of this power supply is from USB of
the computer-based image processor, and it does not have the power switch
itself.
Turn on the laser switch after the laser unit was connected. Connection after the
power being ON may destroy the laser diodes.
Front Rear
The Laser SW is for ON/OFF of the laser light. The 6-pin laser cable is
connected between the Laser unit and the Connector for laser unit.
The USB connectors have IN and OUT. Connect the CCD camera cable from
the CCD camera to the IN, and connect the male-male USB cable from the OUT
to the computer-based image processor. The IN and OUT is connected inside
the power supply, and the CCD camera is not damaged by misconnection.
Connect the USB power cable to the Power connector.
USB power cable 6-pin laser cable CCD camera cable
Connector for
Laser unit
USB connectors for
CCD camera and the
computer
Power connector
Laser SW

6
3) CCD camera and Lens
The images are captured with the CCD
camera. The camera is held on the hold
plate.
The hybrid filter is attached on the lens.
This is the specialized filter combined of
the polarizer and the color filter not to
detect the direct reflection from tissue
surface and the light from outside.
The filter has the direction of
polarization, and it is shown as the white
dot.
Make the white dot showing the front.
50 mm lens
25 mm lens
The standard iris for 50 mm is “ 4 “, and “ 5.6 “ for 25 mm lens. The iris of 5.6 is
the white dot between 4 and 8.
Two space adjusters (spacer) are included. One is the 10 mm and the other is
the 5 mm. Insert the spacer between the CCD camera and the lens. The
measuring area matrix by the set of the lens and the spacer is shown in
Appendix.
10 mm 5 mm
Hold plate
CCD
camera
Iris
・
White dot
Hybrid filter

7
4) Laser unit
The laser diode (wavelength : 780 nm) is installed in the unit.
The shutter is attached on the bottom.
Pull the shutter knob to open.
※ Shut the shutter when the
measurement is over to not to
collect dust on the lens inside.
5) Pole stand
The Hold plate with the CCD camera, the lens and the laser unit is fixed.
Adjust the working distance (WD), distance between the lens and the tissue
under study, according to the size of the tissue.
Use the SUS board under the Magnet base and make the magnet ON so that
the Stand does not fall down.
Spread the black sponge sheet under the animal when the tissue does not
occupy the display to obtain clear blood flow images.
6) USB key
This key allows the blood flow imager software, LSI and LIA, to work.
Shutter
knob
Magnet base
Cramp
2 Poles
Stopper
Hold plate

8
2. Set up of the Pole stand
The Pole stand is composed of the magnet base, the cramp, the stopper and 2
poles.
(1) Insert the longer pole to the
magnet base. Fix the pole by the
screw on the upper position.
(2) Put the cramp through the longer
pole. Insert the shorter pole to the
cramp.
(3) Attach the Hold plate to the
shorter pole by screwing.

9
(4) Install the Laser unit on the Hold
plate by the exclusive screw.
(5) Turning the knurling part can be used
for fine adjustment of the working distance.
The range is 10 mm.
(6) Connect the CCD camera cable and
the laser cable to the CCD camera and the
Laser unit.
アーム
Exclusive screw