Onda V-10 PLUS User manual

VISIBLE
SPECTROPHOTOMETER
V-10 PLUS
User’s Manual

I
Contents
Safety.........................................................................................................................................1
Package Contents ....................................................................................................................1
Unpacking .................................................................................................................................1
Installation.................................................................................................................................2
1. Environment Required................................................................................................ 2
2. Install Spectrophotometer .......................................................................................... 2
Overview....................................................................................................................................2
Symbols.....................................................................................................................................2
Main Specifications..................................................................................................................3
Description of Appearance and Keys.....................................................................................4
1. Appearance................................................................................................................ 4
2. Keypad ....................................................................................................................... 5
3. Description of Keys..................................................................................................... 5
Functions...................................................................................................................................6
Getting Started..........................................................................................................................6
Important Guidelines................................................................................................................7
General Operating ....................................................................................................................7
Measuring..................................................................................................................................8
1. Photometry ................................................................................................................. 8
2. Quantitation................................................................................................................ 9
3. Utility......................................................................................................................... 12
Troubleshooting .................................................................................................................... 14
Repair and Maintenance....................................................................................................... 15
1. Daily Maintain........................................................................................................... 15
2. Spare Parts Replacement ........................................................................................ 15

II
Warranty ................................................................................................................................. 19
Equipment Disposal.............................................................................................................. 19

1
Safety
Please follow the guidelines below, and read this manual in its entirety to ensure safe operation
of the unit.
We recommends against the use of UV-20 Spectrophotometer.
Do not open the device.
Disconnect the device from the mains supply before carrying out
maintenance work or changing the fuses.
The inside of the device is a high-voltage area Danger!
Do not use the device if it is damaged, especially if the main power cable
is in any way damaged or defective.
Repairs may only be carried out by the service technicians from us and
authorized contractual partners.
The device must be connected to a power outlet that has a protective
ground connection.
If the equipment is used in a manner not specified by the manufacturer,
the protection provided by the equipment may be impaired.
Do not allow any liquid to enter into the device.
Do not operate the device in a hazardous location or potentially explosive
environment.
Package Contents
Description
Quantity
Spectrophotometer
1PC
10mm Glass Cuvette
4PCS
Power Cord
1PC
User’s Manual
1PC
Dust Cover
1PC
Unpacking
Open the package, according to carefully check the packaging packing list items, if found inside
the packaging are missing or damaged items please contact us and authorized contractual
partners.

2
Installation
1. Environment Required
To ensure the best performance, the following conditions are required:
The best working temperature range is 16—35℃and the humidity is 45—80%.
Keep it as far as possible away from the strong magnetic or electrical fields or any
electrical device that may generate high-frequency fields.
Set the unit up in an area that is free of dust, corrosive gases and strong vibrations.
Remove any obstructions or materials that could hinder the flow of air under and
around the instrument.
The power requirement is 110±11V/60±1Hz or 220±22V/50±1Hz.
Use the appropriate power cord and plug into a grounded outlet.
If the local voltage is not stable, a voltage regulator is required.
Be away from direct sunlight.
2. Install Spectrophotometer
Placement
Place the instrument on the stable table carefully.
Install Printer (Printer is Optional Accessories)
Check to confirm instrument power switch is turned off, connect the printer’s data cable to the
instrument’s parallel port.
Link the Power Cord
Check to confirm instrument power switch is turned off, the power cord plug into two separate
power interface and power supply socket apparatus.
Overview
UV-20 Spectrophotometer is an electrical measure instrument which is widely used in the
laboratories.
Use Frequency: Intermittence
Excessive Voltage(Current): No
Pollution Class: Class 1
Symbols
The following chart is an illustrated glossary of the symbols that are used in this manual.

3
Caution, Danger!
Caution, High Voltage!
Caution, Hot!
Ground
Fuse
Recycle, this instrument will be called back by the appointed
Electrical Treatment Department or by the original Manufacturer
when wasted.
Main Specifications
Optical System Single beam
Wavelength Range 325—1000nm
Wavelength Accuracy ±2nm
Wavelength Repeatability 0.8nm
Photometric Range -0.3—3A, 0—200%T
Photometric Accuracy ±0.5%T
Photometric Repeatability 0.3%T
Spectral Bandwidth 4nm
Stray Light 0.3%T@360nm
Stability ±0.002A/h@500nm
Work Mode Photometry, Quantitation
Interface USB, Parallel(printer)
Power Requirement AC 110/220V, 50/60Hz
Dimensions 490x360x210
Weight 14kg
Work Environment 15—35℃, 15—70% relative humidity
Store Environment -10—50℃, 15—70% relative humidity

4
Description of Appearance and Keys
1. Appearance
Front View
1
2
3
4
Back View
510
9
8
7
6
11
1 LCD Display
2 Keypad
3 Lid of Sample Room
4 Rod
5 LCD ContrastAdjust
6 Printer port
7 USB port
8 Cover of Fan
9 Power Socket
10 Power Switch
11 Cover of Cooling Vents

5
2. Keypad
3. Description of Keys
SET
SET Key: Set Parameters
GOTO λ
GOTO ʎKey: Set Wavelength
ZERO
ZERO Key: Blank
PRINT
PRINT Key: Print measuring result

6
SELECT
Function Key: Functions according to the screen
▲
,
▲
UP, DOWN Keys: Scroll menu/data and set Y scale
Functions
Photometry
Display results as Abs, %T or Energy.
Quantitation
Use a Standard Sample to establish Standard Curve.
Getting Started
The following chart describes the basic operation of the instrument.
Turn On and Self-check
Switch on the power. Then the instrument
begins to self-check and 20 minutes’ warm up.
Self-check includes the following steps: Turn
on lamps → Check Sensor → Initialize AD →
System position → Get Dark Current → Warm
up.
Sel f - t est . . .
War m up 20 mi nut es,
Any key t o ski p
After warm up, instrument displays Main
Interface.
WL: 500. 0nm 0. 000A
100. 0%T
Basi c Quant i t at i ve

7
Important Guidelines
Reagents and dilution buffers can cause cauterization and other damage to health.
Samples (nucleic acids, proteins, bacteria cultures) can be infectious and cause serious
damage to health.
During sample preparation, measuring procedures and maintenance and cleaning work,
observe all local laboratory safety precautions (e.g. wear protective clothing and gloves,
use of disinfectant) regarding the handling of sample material.
Dispose of measuring solutions and cleaning and disinfectant materials in accordance with
the relevant local laboratory regulations.
General Operating
Select Application
Main interface, press the key
SELECT
(left) to enter into.
Set Wavelength
Test interface, press key
GOTO λ
to set wavelength,
▲
,
▲
to modify wavelength value,
then press key
SELECT
(left) to go to wavelength and blank.
WL: 500. 0nm
Pl ease ent er WL. :
500. 0 nm
OK
Set Parameters
Press
SET
enter into setup interface,
▲
,
▲
to select items or input parameters,
SELECT
(left) to confirm.
Delete the test result and stored data
Test Interface, press the key
PRINT
, then press key
▲
,
▲
to select “Clear Data, not
Print ”,
SELECT
(left) to delete.

8
Blank
Put the Reference in the light path, press
ZERO
to do blank.
Measure Samples
Put the samples in the light path, press
SELECT
(left) to measure.
Print the test results
Test Interface, press the key
PRINT
,
▲
,
▲
select “Print, clear data”, press the key
SELECT
(left) to print.
Store the Standard Curve
After got the Standard Curve by Marked, press
▲
,
▲
input the file name and press
SELECT
(left) to save.
Load the Standard Curve
“Quantitative”interface, press
▲
,
▲
to select “Load Curve”, press
▲
,
▲
to
choose the curve you want, press
SELECT
(left) to open.
Measuring
1. Photometry
Step 1. Start Photometry
Main Interface, press key
SELECT
(left) to choose “Basic”.
WL: 500. 0nm 0. 000A
100. 0%T
Basi c Quant i t at i ve
WL: 500. 0nm 0. 000A
No. WL %T Abs.
Test Cancel
Step 2. Set Wavelength

9
Press
GOTO λ
to set wavelength, press
▲
,
▲
to input wavelength value, press
SELECT
(left) to go to wavelength.
Step 3. Blank
Put the Reference in the light path and press
ZERO
to do blank.
Step 4. Measure samples
Put the sample in the light path, and then the result displays on the screen automatically,
press
SELECT
(left) to record.
WL: 500. 0nm 0. 000A
No. WL %T Abs.
1 500. 0 100. 0 0. 000
2 500. 0 100. 0 0. 000
Test Cancel
2. Quantitation
Step 1. Start Quantitation
Main Interface, press key
SELECT
(left) to choose “Quantitative”.
Cr eat e Cur ve
Load Cur ve
Cl ear Cur ve
OK Cancel
WL: 500. 0nm 0. 000A
100. 0%T
Basi c Quant i t at i ve
Step 2. Establish or call Standard Curve
Cr eat e Cur ve
Load Cur ve
Cl ear Cur ve
OK Cancel
Coef f i ci ent
St andar d Cur ve
OK Cancel
WL: 500. 0nm
2 methods to establish Standard Curve:

10
Method 1: Establish Standard Curve by inputting coefficients
1) Starts establish. Press
▲
,
▲
to select “Coefficient” , then press
SELECT
(left) to confirm.
Coef f i ci ent
St andar d Cur ve
OK Cancel
WL: 500. 0nm WL: 500. 0nm
Wor k WL. :
500. 0 nm
OK Cancel
2) Set wavelength. Press
▲
,
▲
to input test wavelength value, press
SELECT
(left) to confirm.
3) Set coefficient K and B. Press
▲
,
▲
to input coefficient K, press
SELECT
(left), same way set B.
WL: 500. 0nm
Wor k WL. :
500. 0 nm
OK Cancel
WL: 500. 0nm
Coef f i ci ent K:
00000
OK Cancel
Method 2: Establish Standard Curve by using Standard Samples
1) Starts establish. Press
▲
,
▲
to select “Standard Curve”, then press
SELECT
(left).
Coef f i ci ent
St andar d Cur ve
OK Cancel
WL: 500. 0nm WL: 500. 0nm 0. 000A
Pl ease i nser t Bl ank:
OK Cancel
2) Set Wavelength. Press
GOTO λ
to enter to set wavelength, Press
▲
,
▲
to
input wavelength value, press
SELECT
(left) to go the setting value.

11
3) Blank. Put the Reference in the light path, press
SELECT
(left) to do blank.
4) Setup number of Standard Samples. Press
▲
,
▲
to input the quantity of
standard sample(No more than 9 standard curve.), press
SELECT
(left) to confirm.
WL: 500. 0nm
Coef f i ci ent K:
00000
OK Cancel
WL: 500. 0nm
Number : 3
OK Cancel
5) Calibrate Standard Samples. Put the corresponding standard samples in the light
path as the screen indicates, press
▲
,
▲
to input the concentration, press
SELECT
(left) to confirm, to finish all the standard samples.
WL: 500. 0nm
Number : 3
OK Cancel
WL: 500. 0nm
I nser t 1# St andar d.
I nput 1# Conc: 00000
OK Cancel
Load the Stored Curve
Press
▲
,
▲
to choose “Load Curve”, press
▲
,
▲
to choose the curve,
press
SELECT
(left) to confirm.
Cr eat e Cur ve
Load Cur ve
Cl ear Cur ve
OK Cancel
C=1. 000xA+0. 2 500. 0 <
C=0. 02xA+0. 32 470. 0
OK Cancel
C=KxA+B WL.
Press
SELECT
(left) to enter the test mode after building or loading standard curve.

12
Step 3. Enter into Measuring Interface
Press
SELECT
(left) to enter into the Quantitation Measuring Interface.
r =0. 996, C=2. 267+1. 211
- 0. 100 0. 900
4. 000
0. 000
Test Cancel
+
+
+
WL: 500. 0nm 0. 000A
No. WL Abs. Conc.
Test Cancel
Step 4. Blank
Put the Reference in the light path, press
ZERO
to do blank.
Step 5. Measure Samples
Put the sample to be tested in the light path, press
SELECT
(left) to measure. Then the test
result will display in the data sheet. Repeat this step to finish measuring all the samples.
500. 0nm 0. 000A
No. WL Abs Conc.
1 500. 0 0. 039 0. 078
2 500. 0 0. 042 0. 084
3 500. 0 0. 041 0. 082
3. Utility
Main Interface, press
SET
to go into utility setting.
Test Mode
D2 Lamp On
W Lamp On
OK Cancel
WL: 500. 0nm 0. 000A
100. 0%T
Basi c Quant i t at i ve
Test Mode
Press
▲
,
▲
to choose “Test Mode”, press
SELECT
(left) to enter, press
▲
,
▲
to

13
choose “Abs”, “%T”, “Energy”, press
SELECT
(left) to confirm.
Test Mode
D2 Lamp On
W Lamp On
OK Cancel
Abs
%T
Ener gy
OK Cancel
Turn On/Off D2 Lamp
Press
▲
,
▲
to choose “D2 Lamp”, then press
SELECT
(left) to enter into. Press
▲
,
▲
to choose “On”or “Off”, press
SELECT
(left) to turn on/off.
√D2 Lamp On
On √
Of f
Test Mode
D2 Lamp On
W Lamp On
OK Cancel OK Cancel
Turn On/Off W Lamp
Press
▲
,
▲
to choose “W Lamp”, then press
SELECT
(left) to enter into. Press
▲
,
▲
to choose “On”or “Off”, press
SELECT
(left) to turn on/off.
√W Lamp On
On √
Of f
Test Mode
D2 Lamp On
W Lamp On
OK Cancel OK Cancel
Get Dark Current
Keep the light path without anything blocking, press
▲
,
▲
to choose “Dark Current”,
then press
SELECT
(left) to resample Dark Current.
Note: During the course, open the lid of the compartment is prohibited.

14
Get Dar k Cur r ent !
Dar k Cur r ent
Reset WL
Ver si on
OK Cancel
Reset Wavelength
Keep the light path without anything blocking, press
▲
,
▲
to choose “Reset WL”, then
press
SELECT
(left) to reset wavelength.
Cal i br at i ng WL!
Dar k Cur r ent
Reset WL
Ver si on
OK Cancel
About Version
Press
▲
,
▲
to choose “Version”, press
SELECT
(left) to view version information, press
any key to return.
Sof t war e: V2. 4. 2
Har dwar e: V1. 2
Dar k Cur r ent
Reset WL
Ver si on
OK Cancel
Troubleshooting
Review the information in the table below to troubleshoot operating problems.
Problem
Cause
Solution
Power on, no response
Power cord connection is not
reliable
Improve connectivity
Fuse burning
Replace fuse
Measurement uncertainty
Warm up is not enough
Warm up more time

15
Sample is not Stable
Improve the sample
The concentration of sample is
too high
Diluted sample
Power Supply Voltage Low or
not Stable
Improve the Power Supply
Lamp damage or lamp life
maturity
Replace lamp
Dark Current Error when self-
check
The lid of the compartment is
open during self-check
Close the lid, restart
System Calibrate Failed
Something block the Light path
Remove it, calibrate again
Power on, back light is OK, but
nothing display on the screen or
display is not clear
Display Contrast problem
Adjust the contrast
potentiometer
Measurements inaccurate
Cuvettes were contaminated
Clean cuvettes
Samples were contaminated
Improve samples
Worse matching of the cuvettes
Improve the matching of the
cuvettes
Dark current error
Resample dark current
Repair and Maintenance
1. Daily Maintain
Check the Compartment
After measurement, the cuvettes with sample solutions should be taken out of the compartment
in time. Or the volatilization of the solution would make the mirror go moldy. Users must pay
more attention to the corrosive sample and liquid easy to volatilize. Any solution remains in the
compartment should be wiped off immediately.
Surface Clean
The cover of the instrument is with paint. Please use wet towel to wipe off the drips on the
surface immediately. Organic solution is forbidden to be used to clean the cover. Please wipe
off the dirt on the cover timely.
Clean the Cuvettes
After every test or after a solution change, the cuvettes should be cleaned carefully, or the
remains on the surface would cause measuring error.
2. Spare Parts Replacement
Replace the Fuse

16
Danger! Be sure to switch off the power and unplug the socket before
replacement!
Step 1. Tools preparation
Prepare a 3×75 Flat Blade screwdriver.
Step 2. Switch Off the power supply
Switch off the power supply, and unplug the socket.
Step 3. Take out the Fuse Seat
Take out the fuse seat by the screwdriver.
Step 4. Replace a new fuse
Pick out the spare fuse (3.15A/250V) and replace it to the working position.
1
2
Step 5. Reset the fuse seat
Replace the fuse seat in the power socket.
Step 6. Switch on the power
Plug the socket and switch on the power.

17
Replace Lamps
Hot! Wait 20 minutes before open the lamp chamber after power off to
avoid scald!
Step 1. Tools preparation
Prepare a 6×150mm Cross Blade screwdriver and a pair of glove.
Step 2. Power Off
Switch off the power supply and unplug the socket.
Step 3. Open the cover
Unscrew the 4 screws indicated(Each side with 2 screws) and remove the cover.
Rod
Step 4. Open the cover of the light chamber
Unscrew the 2 screws on the light chamber cover and remove it.
Light
chamber
Step 5. Replace W lamp
Pull out the defected W lamp and draw on the cotton glove. Insert the new W lamp as deep
as possible on the lamp seat. Be sure to keep the filament in the same direction as the old
one face.