Onda V-10 PLUS User manual

VISIBLE
SPECTROPHOTOMETER
V-10 PLUS
User’s Manual

I
Contents
Safety.........................................................................................................................................1
Package Contents ....................................................................................................................1
Unpacking .................................................................................................................................1
Installation.................................................................................................................................2
1. Environment Required................................................................................................ 2
2. Install Spectrophotometer .......................................................................................... 2
Overview....................................................................................................................................2
Symbols.....................................................................................................................................2
Main Specifications..................................................................................................................3
Description of Appearance and Keys.....................................................................................4
1. Appearance................................................................................................................ 4
2. Keypad ....................................................................................................................... 5
3. Description of Keys..................................................................................................... 5
Functions...................................................................................................................................6
Getting Started..........................................................................................................................6
Important Guidelines................................................................................................................7
General Operating ....................................................................................................................7
Measuring..................................................................................................................................8
1. Photometry ................................................................................................................. 8
2. Quantitation................................................................................................................ 9
3. Utility......................................................................................................................... 12
Troubleshooting .................................................................................................................... 14
Repair and Maintenance....................................................................................................... 15
1. Daily Maintain........................................................................................................... 15
2. Spare Parts Replacement ........................................................................................ 15

II
Warranty ................................................................................................................................. 19
Equipment Disposal.............................................................................................................. 19

1
Safety
Please follow the guidelines below, and read this manual in its entirety to ensure safe operation
of the unit.
We recommends against the use of UV-20 Spectrophotometer.
Do not open the device.
Disconnect the device from the mains supply before carrying out
maintenance work or changing the fuses.
The inside of the device is a high-voltage area Danger!
Do not use the device if it is damaged, especially if the main power cable
is in any way damaged or defective.
Repairs may only be carried out by the service technicians from us and
authorized contractual partners.
The device must be connected to a power outlet that has a protective
ground connection.
If the equipment is used in a manner not specified by the manufacturer,
the protection provided by the equipment may be impaired.
Do not allow any liquid to enter into the device.
Do not operate the device in a hazardous location or potentially explosive
environment.
Package Contents
Description
Quantity
Spectrophotometer
1PC
10mm Glass Cuvette
4PCS
Power Cord
1PC
User’s Manual
1PC
Dust Cover
1PC
Unpacking
Open the package, according to carefully check the packaging packing list items, if found inside
the packaging are missing or damaged items please contact us and authorized contractual
partners.

2
Installation
1. Environment Required
To ensure the best performance, the following conditions are required:
The best working temperature range is 16—35℃and the humidity is 45—80%.
Keep it as far as possible away from the strong magnetic or electrical fields or any
electrical device that may generate high-frequency fields.
Set the unit up in an area that is free of dust, corrosive gases and strong vibrations.
Remove any obstructions or materials that could hinder the flow of air under and
around the instrument.
The power requirement is 110±11V/60±1Hz or 220±22V/50±1Hz.
Use the appropriate power cord and plug into a grounded outlet.
If the local voltage is not stable, a voltage regulator is required.
Be away from direct sunlight.
2. Install Spectrophotometer
Placement
Place the instrument on the stable table carefully.
Install Printer (Printer is Optional Accessories)
Check to confirm instrument power switch is turned off, connect the printer’s data cable to the
instrument’s parallel port.
Link the Power Cord
Check to confirm instrument power switch is turned off, the power cord plug into two separate
power interface and power supply socket apparatus.
Overview
UV-20 Spectrophotometer is an electrical measure instrument which is widely used in the
laboratories.
Use Frequency: Intermittence
Excessive Voltage(Current): No
Pollution Class: Class 1
Symbols
The following chart is an illustrated glossary of the symbols that are used in this manual.

3
Caution, Danger!
Caution, High Voltage!
Caution, Hot!
Ground
Fuse
Recycle, this instrument will be called back by the appointed
Electrical Treatment Department or by the original Manufacturer
when wasted.
Main Specifications
Optical System Single beam
Wavelength Range 325—1000nm
Wavelength Accuracy ±2nm
Wavelength Repeatability 0.8nm
Photometric Range -0.3—3A, 0—200%T
Photometric Accuracy ±0.5%T
Photometric Repeatability 0.3%T
Spectral Bandwidth 4nm
Stray Light 0.3%T@360nm
Stability ±0.002A/h@500nm
Work Mode Photometry, Quantitation
Interface USB, Parallel(printer)
Power Requirement AC 110/220V, 50/60Hz
Dimensions 490x360x210
Weight 14kg
Work Environment 15—35℃, 15—70% relative humidity
Store Environment -10—50℃, 15—70% relative humidity

4
Description of Appearance and Keys
1. Appearance
Front View
1
2
3
4
Back View
510
9
8
7
6
11
1 LCD Display
2 Keypad
3 Lid of Sample Room
4 Rod
5 LCD ContrastAdjust
6 Printer port
7 USB port
8 Cover of Fan
9 Power Socket
10 Power Switch
11 Cover of Cooling Vents

5
2. Keypad
3. Description of Keys
SET
SET Key: Set Parameters
GOTO λ
GOTO ʎKey: Set Wavelength
ZERO
ZERO Key: Blank
PRINT
PRINT Key: Print measuring result

6
SELECT
Function Key: Functions according to the screen
▲
,
▲
UP, DOWN Keys: Scroll menu/data and set Y scale
Functions
Photometry
Display results as Abs, %T or Energy.
Quantitation
Use a Standard Sample to establish Standard Curve.
Getting Started
The following chart describes the basic operation of the instrument.
Turn On and Self-check
Switch on the power. Then the instrument
begins to self-check and 20 minutes’ warm up.
Self-check includes the following steps: Turn
on lamps → Check Sensor → Initialize AD →
System position → Get Dark Current → Warm
up.
Sel f - t est . . .
War m up 20 mi nut es,
Any key t o ski p
After warm up, instrument displays Main
Interface.
WL: 500. 0nm 0. 000A
100. 0%T
Basi c Quant i t at i ve

7
Important Guidelines
Reagents and dilution buffers can cause cauterization and other damage to health.
Samples (nucleic acids, proteins, bacteria cultures) can be infectious and cause serious
damage to health.
During sample preparation, measuring procedures and maintenance and cleaning work,
observe all local laboratory safety precautions (e.g. wear protective clothing and gloves,
use of disinfectant) regarding the handling of sample material.
Dispose of measuring solutions and cleaning and disinfectant materials in accordance with
the relevant local laboratory regulations.
General Operating
Select Application
Main interface, press the key
SELECT
(left) to enter into.
Set Wavelength
Test interface, press key
GOTO λ
to set wavelength,
▲
,
▲
to modify wavelength value,
then press key
SELECT
(left) to go to wavelength and blank.
WL: 500. 0nm
Pl ease ent er WL. :
500. 0 nm
OK
Set Parameters
Press
SET
enter into setup interface,
▲
,
▲
to select items or input parameters,
SELECT
(left) to confirm.
Delete the test result and stored data
Test Interface, press the key
PRINT
, then press key
▲
,
▲
to select “Clear Data, not
Print ”,
SELECT
(left) to delete.