Origio MARS 900 CLASS II User manual

Mars Workstation
User Manual

Version 4.2: Aug 26, 2015

Version 4.2: Aug 26, 2015 3
Dear User,
Congratulations on your purchase of a MARS workstation. This device has been tested and certified to protect your
samples from particles.
The user’s manual is meant to provide you with instruction on how to use the MARS workstation safely and
effectively.
The MARS workstation provides you with unique features that will enable you to perform your IVF processes safely
and effectively.
For any further questions on using this product or need further explanation of any of its features, please contact
ORIGIO A/S, or your local distributor.

4 Version 4.2: Aug 26, 2015
INDEX
I.
HOW TO USE THIS MANUAL..............................................................................................................................7
a.
Standard and safety regulations.......................................................................................................................8
b.
General Notes...................................................................................................................................................8
c.
Intended use.....................................................................................................................................................8
d.
Symbols used in the user manual.....................................................................................................................9
e.
Warranty and Liability .....................................................................................................................................10
f.
Safety Instructions ..........................................................................................................................................11
g.
Symbols on the equipment .............................................................................................................................13
II.
DELIVERY...........................................................................................................................................................14
a.
Standard Components....................................................................................................................................14
b.
Inspection and acceptance.............................................................................................................................14
c.
Transport.........................................................................................................................................................15
III.
INSTALLATION ..............................................................................................................................................16
a.
Ambient Locations ..........................................................................................................................................16
Location requirements:............................................................................................................................................16
b.
Room Ventilation:............................................................................................................................................17
c.
Installation test................................................................................................................................................17
h.
Accessories.....................................................................................................................................................17
•
Light source TLB 7000:...................................................................................................................................18
•
Heated Glass HG37:.......................................................................................................................................18
•
Humidifier:.......................................................................................................................................................19
•
LED / LCD Monitor:.........................................................................................................................................20
Integrated microscope(s):...............................................................................................................................20
•...............................................................................................................................................................................20
•
Camera(s) for the stereomicroscope(s):.........................................................................................................25
•
Liquid Bath Heating Controller:.......................................................................................................................26
•
Anti-vibration Table:........................................................................................................................................27
IV.
DEVICE DESCRIPTION.................................................................................................................................28
a.
Overall view ....................................................................................................................................................29
b.
Inner overview.................................................................................................................................................31
c.
Air Flow Principle............................................................................................................................................32
d.
Device interfaces ............................................................................................................................................33
e.
Chamber Illumination......................................................................................................................................33
f.
Working Area..................................................................................................................................................33
g.
UV Light ..........................................................................................................................................................33
h.
Front Window..................................................................................................................................................34
V.
CONTROL PANEL..........................................................................................................................................35
A.
DISPLAY.........................................................................................................................................................35

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Switching fans ON / OFF at normal speed .............................................................................................................35
Switching fans ON / OFF at reduced speed ...........................................................................................................35
Alarm...............................................................................................................................................................36
a.
Internal light ....................................................................................................................................................36
b.
UV light ...........................................................................................................................................................36
c.
Heated surface, ORIGIO light source, and heated glass stage .....................................................................37
B.
CONTROL & PROGRAMMING......................................................................................................................37
I.
Overview of the Control Panel Menu..............................................................................................................38
II.
General air flow information & counter ...........................................................................................................38
III.
Adjusting the level intensity of the internal light..............................................................................................39
IV.
Programming and Controlling the UV light timer........................................................................................40
UV Autostart Option Selected: “TODAY”.............................................................................................................41
UV Autostart Option Selected: “AUTO”...............................................................................................................41
V.
Programming Time and Date..........................................................................................................................43
VI.
Programming the Air Flow to auto start (Weekly or Daily)..........................................................................44
Option selected: TODAY.....................................................................................................................................45
Option selected: AUTO........................................................................................................................................45
VII.
Setting up The timer function to control heating, light source TLB7000 and Heated glass HG37.............47
VIII.
Display Mode Functions .............................................................................................................................49
V.
OPERATION...................................................................................................................................................51
a.
Preparation for the sample chamber ..............................................................................................................51
b.
Working in the sample chamber.....................................................................................................................51
VI.
CLEANING and DECONTAMINATION..........................................................................................................52
a.
Decontamination procedure:...........................................................................................................................52
b.
Wipe/spray disinfection...................................................................................................................................52
Pre-disinfection: ......................................................................................................................................................52
Cleaning:.................................................................................................................................................................53
Final disinfection: ....................................................................................................................................................53
c.
UV disinfection:...............................................................................................................................................53
d.
Cleaning of the exterior surfaces....................................................................................................................53
e.
Cleaning of the floor pan.................................................................................................................................53
f.
Cleaning of the humidifier...............................................................................................................................54
VII.
SHUT-DOWN..................................................................................................................................................55
a.
Interrupting an operation.................................................................................................................................55
b.
Shutting the unit down ....................................................................................................................................55
IX.
DECOMMISSIONING.....................................................................................................................................56
X.
TROUBLESHOOTING Your AIR FLOW ........................................................................................................57
a.
Flow 1 alarm ...................................................................................................................................................57
XI.
HEATED SURFACE.......................................................................................................................................59
Operational Characteristics.....................................................................................................................................59

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a.
Temperature Controller...................................................................................................................................60
b.
User Setup......................................................................................................................................................62
Accessing the parameters and information menu...............................................................................................62
Resetting the THI and TLO recordings................................................................................................................62
Channel 1 set point..............................................................................................................................................62
Standby................................................................................................................................................................63
Keypad lock.........................................................................................................................................................63
c.
Operating the heated areas............................................................................................................................63
Normal Operation ................................................................................................................................................63
Checking the temperature...................................................................................................................................63
XI.
TECHNICAL SPECIFICATIONS ....................................................................................................................64
a.
MARS 900 IVF or LAF....................................................................................................................................64
b.
MARS 1200 IVF or LAF..................................................................................................................................65
c.
MARS 1500 IVF or LAF..................................................................................................................................66
d.
MARS 1800 IVF or LAF..................................................................................................................................67
e.
MARS 1800 Dual............................................................................................................................................68
f.
MARS 1800MP...............................................................................................................................................69
XII.
MAINTENANCE..............................................................................................................................................70
a.
Field Certification............................................................................................................................................70
b.
Service............................................................................................................................................................70
HEPA Filter replacement:........................................................................................................................................70
Sample chamber illumination tube..........................................................................................................................70
UV Lamp .................................................................................................................................................................71
Retrofitting and repairs............................................................................................................................................71
XIII.
ROUTINE MAINTENANCE SCHEDULE........................................................................................................72
a.
Weekly............................................................................................................................................................72
b.
Monthly (or more often as required) ...............................................................................................................72
c.
Quarterly or Semiannually ..............................................................................................................................72
d.
Annually..........................................................................................................................................................72
XIV.
CERTIFICATING TESTING............................................................................................................................73
a.
Test Terms......................................................................................................................................................73
b.
Test.................................................................................................................................................................73
c.
Test equipment...............................................................................................................................................73
XV.
SPARE PARTS...............................................................................................................................................74

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I. HOW TO USE THIS MANUAL
This manual is only meant as a guide on the operation of the workstation for the user, who uses the workstation as
part of their regular procedures in the laboratory.
It does not contain information with regard to the technical service, maintenance, and details of design, production,
or every possible situation which may arise during installation. This work or actions are to be completed by a
certificated or technically trained engineer approved by ORIGIO or our authorized distribution network.
To reduce the risk of fire or electric shock, do not disassemble or remove any covers as there are no user
serviceable parts inside.
Repair should be done by authorized service personnel only.
Correct Use
The following procedures must always be followed:
•Prior to the initial operation of the workstation, the operator must perform an installation test. The test result
must be documented by a test report. The installation test must be performed by a qualified technician or a
certified company.
•The use of the product will be confined to trained and expert users
•Keep these operating instructions close to the unit so that safety instructions and important information are
always accessible.
•The operator must prepare clear standard operating instructions in the language of the operating and
cleaning personnel based on these operating instructions, applicable safety data sheets, plant hygiene
guidelines, and technical regulations, in particular:
oWhich decontamination measures are to be applied to the MARS workstation and accessories,
oWhich protective measures apply while specific agents are used or while specific processes are
conducted,
oWhich measures are to be taken in the case of an accident.
•Should you encounter problems that are not properly detailed adequately in these operating instructions,
please contact ORIGIO immediately for your own safety.
•If this manual is translated into other foreign languages, the English version of these operating instructions
is a biding lease.
•After any changes to the installation conditions or after any modification to the technical system, a repeat
test must be performed and the test result must be documented by a test report.
Incorrect Use
The MARS workstation must not be used as a biosafety cabinet in laboratories that do not comply with the
requirements of safety levels 1, 2, 3 as described in Bio-safety in Microbiological and Biomedical Laboratories.
(The reference is Department of Health and Human Services, National Institute of Health, 6705 Rockledge Drive,
Suite 750, MSC 7985, Bethesda, MD 20892-7985 www.nih.gov).
•The workstation must not be operated if no repeat test is performed at the installation or after changes
in the installation conditions or modifications to the technical system, as the protection provided by the
equipment may be impaired.
•The workstation must not be operated if the alarm system of the device has issued a failure message
and the cause for the failure has not been repaired, as the protection provided by the equipment may
be impaired.
•The alarm system must not be tampered with or disabled. If alarm system components have been
removed or disabled for service or repairs, the unit must only be released for operation if all alarm
system components are functioning again properly.
•The filters installed in the workstation are not capable of separating gaseous substances. Do not work
with or store substances in the device:
oWhich in quantity or concentration are toxic,
oIf a reaction with other substances may result in hazardous toxic concentrations or formation of
toxic gases, which may form combustible or explosive mixtures in combination with air.

8 Version 4.2: Aug 26, 2015
a. Standard and safety regulations
The device complies with the safety requirements of the following standards and guidelines:
USA/Canada
• IEC 61010-2-010
• IEC 61010 3’rd edition
• NSF/ANSI Standard 49/2009
Europe / Middle East / Africa
• IEC 61010-2-010
• IEC 61010 3’rd edition
• EN 12469 / 2000
• Low Voltage Directive
• EMC Directive
Australia /Asia / Pacific
• IEC 61010-2-010
• IEC 61010 3’rd edition
b. General Notes
The address of ORIGIO Headquarter is:
ORIGIO A/S
Knardrupvej 2
2760 Måløv Denmark
For inquiries call Customer Service: +45 46 79 02 02 or by email customer@origio.com
c. Intended use
The MARS workstation is a microprocessor controlled laminar airflow Class II biological safety cabinet type A1, for
installation and operation in IVF laboratories of biosafety levels 1, 2 and 3 as described in Biosafety in
Microbiological and Biomedical laboratories (The reference is Department of Health and Human Services, National
Institute of Health, 6705 Rockledge Drive, Suite 750, MSC 7985, Bethesda, MD 20892-7985 www.nih.gov). The
MARS workstation meets EN12469 (The European Standard for microbiological safety cabinets).
The MARS workstation is available as a laminar airflow biosafety cabinet and as a Class II biosafety IVF
workstation. Depending on the model the IVF workstations is available with the option of one or two warmed
surfaces in the table plate with processor controlled heated glass, one or two gas humidified devices, an LCD or
LED monitor, UV Light, and preparation for the integration of microscopes with an integrated light source.

Version 4.2: Aug 26, 2015 9
d. Symbols used in the user manual
Throughout this manual, blocks of text may be accompanied with a pictogram. These blocks are WARNINGS,
CAUTIONS, and IMPORTANT NOTES and they are used as follows:
NOTE
Used to direct attention to a special item or for
useful information
WARNING
DANGER
UV light radiation hazard. Use only with shielding in
place. Protect eyes and skin from exposure to UV light.
FURHTER INFORMATION
Further information is provided in other sections or
manuals.

10 Version 4.2: Aug 26, 2015
e. Warranty and Liability
Limited Warranty
ORIGIO Equipment warrants to the purchasers of all devices and products solely manufactured by ORIGIO.
In the event of product failure under normal use, due to defects in material or workmanship, within a period of
twenty four months (24) months from the date of invoice of the Product and from the point of origin, the product
will be repaired, or at ORIGIO option, replaced, at no charge. ORIGIO assumes that the Purchaser is
experienced in the use of this device and is able to judge from his/her own expertise the suitability or otherwise
of the product for the intended use. This limited warranty does not apply to products subjected to abnormal use
or conditions, improper storage, damaged by accident, misuse or abuse, improper line voltage, products whose
serial number has been altered, to products not shipped in accordance with the recommendations of ORIGIO,
and/or to products altered or serviced by anyone other than ORIGIO authorized distributors. Distributor is
responsible for the labor and travel costs during this period.
This limited warranty is exclusive and in lieu of all other warranties whether written, oral, expressed or implied.
In particular, ORIGIO does not warrant that the product is suitable for the needs of the purchaser and there are
no warranties given as to merchantability or fitness for a particular purpose other than the one specified in
ORIGIO literature that accompanies every specific product.
ORIGIO reserves the right to change or discontinue this product without prior notice.
Liability:
Because ORIGIO EQUIPMENT has no control or influence over the conditions under which this device is used,
over its method of use or administration, or on handling of the product after it leaves its possession ORIGIO
takes no responsibility for the results, use and/or performance of the product. ORIGIO expects that use of the
product will be confined to trained and expert users.
In no event shall ORIGIO be liable for any direct or indirect damages including incidental, consequential or
special damages, arising out of or in connection with the use or performance of the product.
If ORIGIO provides you with technical documentation, this does not authorize you to perform repairs,
adjustments or alterations on the device or accessories.
No representative of ORIGIO and no vendor of the product is authorized to change any of the foregoing terms
and conditions, and the purchaser accepts the product subject to all terms and conditions herein, subject
always to any contrary provisions which are necessarily implied by stature or law notwithstanding the within
terms and conditions.
Replacement:
As mentioned in the Limited Warranty, The decision whether to provide any remedy or whether to refund any
portion of the purchase price shall be at the discretion of ORIGIO.
Before returning a product for any reason, please contact your nearest ORIGIO distributor for assistance and
instructions.

Version 4.2: Aug 26, 2015 11
f. Safety Instructions
To avoid unintended or improper operation of the workstation, please read this manual
carefully
Warning:
The workstation should be certified by a qualified technician or a certified company before its
initial use. The workstation should be recertified whenever it is relocated.
The workstation has to be serviced annually thereafter.
Do not assume that filter integrity and airflow performance have not been compromised during
shipment.
.
To ensure that the working area is clean and sterile the workstation fan must be run at
normal speed for at least 30 minutes prior to working inside the Workstation.
Objects and instruments must be carefully cleaned and/or disinfected before bringing them into
the work chamber.
Necessary instruments for use during work must be placed within reach to avoid unnecessary
movement inside the Workstation.
For reliable operation it is important that the air-flow conditions are as unobstructed as much as
possible. Therefore, never overload the work chamber.
Make sure appropriate personal protective equipment (PPE) is worn, including lab coats and
gloves. Eye protection and face shields can also be worn, as needed. Special attention should
be given to hands and lower parts of the arms, as these are the parts of the operator most likely
to emit particles near the product.
All work in the workstation must be performed with slow movements. Rapid arm movements in
the chamber may cause slipstreams, which will draw contaminated air into the work chamber.
Transport of possibly contaminated material may create airflows which can contaminate the
product.
The device must not be used for working with materials which can cause allergic reactions, or
any harm to the health of the operator or the personnel. Attention is drawn to the risk
assessment requirements of the Control of Substances Hazardous to Health (COSHH)
Regulations 1999. (UK)
The workstation is not suitable for HIGH-RISK biological agents. HIGH RISK biological agents
include all etiologic agents designated Class 4 by CDC, and oncogenic viruses classed high risk
by NCI. (USA)
The workstation will not provide any protection for operator or environment against harmful
gases or vapors.
There are no user-serviceable parts within the housing. Repairs to this equipment should only be
performed by a qualified technician or certified company. For service information, please contact
your nearest ORIGIO distributor for assistance.

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Some internal components of the workstation may become contaminated during normal use of
the unit. Only experienced personnel competent in decontamination procedures or certified
companies should decontaminate the workstation before servicing these components.
Ensure that the workstation is connected to electrical service in accordance with local and
national electrical codes. Failure to do so may create a fire or electrical hazard.
Do not remove or service any electrical components without first disconnecting the workstation
from electrical service
Avoid the use of flammable gases or solvents in the workstation. Care must be taken to ensure
against the concentration of flammable or explosive gases or vapors.
An open flame should NOT be used in the workstation. Open flames disrupt airflow patterns in
the workstation and may burn the HEPA filter and/or damage the filter’s adhesive.
Gases under high pressure should not be used in the workstation, as they may disrupt its airflow
patterns
HEPA filters only remove particulate matter. Operations generating volatile toxic chemicals or
radionuclides must be evaluated carefully and when approved by an appropriate safety offer may
require connection to a dedicated external exhaust through a thimble connection.
The HEPA filter is fragile and should not be touched. Avoid puncturing either HEPA filter during
installation or normal use. If you suspect that a HEPA filter has been damaged, DO NOT use the
workstation; contact a local certification provider.
Proper operation of the cabinet depends largely upon its location and the operator’s work habits.
Consult the Installation and Operation sections of this manual for further details.
Customer materials which are not UV-resistant should not be used during UV-disinfection inside
the work chamber.
The HEPA filter in the workstation will gradually accumulate airborne particulate matter from the
room and from work performed in the workstation. The rate of accumulation will depend upon the
cleanliness of the room air. The airflow sensors will alarm when the HEPA filters will need to be
checked.
The VOC filter placed in the contained box on top of the workstation (optional) has to be
exchanged every 3 to 6 month. The rate of exchange of the VOC filter will depend upon the
cleanliness of the room air. Only experienced personnel competent in decontamination
procedures or certified companies should replace and decontaminate the workstation before
use.
Fuses must be exchanged by a qualified technician or a certified company. Please contact your
nearest ORIGIO EQUIPMENT distributor for assistance.

Version 4.2: Aug 26, 2015 13
g. Symbols on the equipment
Caution – Consult this manual for safety precautions
Biohazard - Observe operating instructions
Caution - High voltage. Possibility of electric shock.
DANGER
UV light radiation hazard. Protect eyes and skin from exposure to UV light.
Protective Earth Symbol
Label indicating lifting points to lift the device for installation
Caution – Pinch point finger
Label Power outlet(s), maximum 200 W per outlet ( 2 outlets per workstation is the
maximum)

14 Version 4.2: Aug 26, 2015
II. DELIVERY
a. Standard Components
Model MARS CLASS II LAF:
• LAF cabinet
• 2x Electrical outlets in the back wall
• Support frame (Legs Assembly and fittings)
Model MARS CLASS II IVF:
• LAF Cabinet with Heated surface and heated surface controller
• Heated Glass and controller (HG37model )
• Support frame (Legs Assembly and fittings)
• Humidifier with one glass bottle per humidifier
• An integrated 21” monitor
• A USB connection
• A shelf for mounting for the Heated Glass
All models:
• Device documentation:
— User manual,
— Factory test report,
— Cleaning and repair kit.
Optional components and accessories will be listed as separate items in the delivery document.
b. Inspection and acceptance
After the device has been delivered, immediately check:
• The device is complete and according to order,
• For possible damage.
Please refer to the “Transport” section for further information about anti-shock and anti-
tilt sensor activation.

Version 4.2: Aug 26, 2015 15
c. Transport
To prevent tilting, always transport the device using a suitable carrier, even for a transport within a building, and
separate it from the stand.
CAUTION:
Do not transport the device over large distances without transport lock and original
device packaging
The device is packed in a wooden like crate mounted and strapped to a wooden pallet. The
workstation is also fixed to the pallet with 2 screws in the back.
For the safety of the device and making sure that the device has been properly handled, anti-
chock and anti-tilt sensors are placed on the exterior of the crate. In case of activation of the
sensors, please notify and report it immediately to your transporter and your local ORIGIO
distributor and inspect the device accordingly for damages.
CAUTION:
Danger of tipping over
For transport, lift the device only using the lift points shown in the illustration. These lift
points are highlighted with a “Lift Here” sticker.
CAUTION: Contusion hazard
When lifting the workstation, do not put hands or fingers between floor pan and frame!
To protect hands from injury, it is optionally recommended to use suitable gloves.
Handling
Indicator
Handling
Indicator

16 Version 4.2: Aug 26, 2015
III. INSTALLATION
CAUTION:
The installation of the workstation must be completed by a certificated or technically
trained engineer approved by ORIGIO or our authorized distribution network
a. Ambient Locations
The operational safety and proper function of the device depend on the location where it is to be used. The device
must be operated only at locations that meet the ambient conditions listed below.
Location requirements:
oThe electrical system of the device has been designed for an operating height of up to 2000 m (6500 ft.)
above sea level.
oThe mains power supply outlets should be out of normal reach to prevent accidental shut-off, but within
easy access for disconnection.
oThe flooring of the location must be adequately strong.
oThe room in which the device is installed must be of adequately height. The distance between the exhaust
air opening and the room ceiling must be at least 200 mm (8 in).
oThe location must be equipped with an appropriate ventilation system.
oThe temperature within the room must be between 15 °C and 35 °C (68 °F and 95 °F)
oThe relative humidity in the vicinity of the device must not exceed 80%.
oChoose a draft-free location where the workstation does not interfere with the room traffic.
oThe device should be placed on a level secure surface, away from heaters, coolers and air-conditioning
outlets.
Temporary Storage: If the device is stored only temporarily (up to four weeks), the
ambient temperature may be between +5°C and 40°C (4 1°F and 104°F). For longer storage
periods, the location requirements apply.
Installation in Line: When several devices are to be installed in line, make sure that
vibrations cannot be transferred between adjacent units. Give 254 mm (10 in) clearance
between the workstations or side proximity to enable cleaning and disinfection.

Version 4.2: Aug 26, 2015 17
b. Room Ventilation:
The room ventilation of the laboratory should preferably be a ventilation system that complies with the national
requirements for the application.
•The inlet air and exhaust air openings of the room ventilation must be located so that drafts are prevented
from impairing the function of the MARS air system.
c. Installation test
Do not operate the equipment before the installation test has been completed.
The installation inspection of the workstation must be conducted by a qualified technician or a certified company. It
is important to validate if the workstation functions listed below were checked and if the results are within the safety
value tolerances specified in the manufacturing test report included in the folder.
Down flow velocity test
Inflow velocity test
HEPA Filter Leak Test
Particle count test
Temperature calibration of the heat surface and the heated glass. (MARS IVF only)
A repeat test must also be performed after repairs to the device or after moving the device
more than 10 cm (4.9 inch).
The operator must request a written test report from the authorized test service.
Safety warranty: The operational safety of the device, particularly the samples, is guaranteed
only if all safety functions of the device have been tested and approved. ORIGIO will not warrant
the operational safety if the device is operated without performance of the required installation
test or if the installation test and repeat test are not performed by adequately trained and
authorized personnel.
Device Hygiene: The installation test does not include any decontamination measures. For
operation in the sample chamber of the workstation, the sample chamber and the accessories
required for the work process must be disinfected and cleaned in accordance with the hygiene
guidelines set forth for the application.
h. Accessories
CAUTION:
The installation of the workstation must be completed by a certificated or technically
trained engineer approved by ORIGIO or our authorized distribution network
There are several accessories that can be either integrated or added to the MARS workstation. These concerns:
Light source: TLB 7000
Heated glass stage: HG37
Humidifier
Integrated Stereomicroscope(s)
Camera(s) for the stereomicroscope(s)

18 Version 4.2: Aug 26, 2015
LED or LCD monitor
Liquid bath circulator
Anti-vibration table
It is important to refer to the installation instructions recommended by the manufacturer.
•Light source TLB 7000:
Depending on the customer order and on the configuration selected, a light source TLB 7000 can be attached to
the bottom of the workstation table plate.
To operate the light source, please refer to the TLB 7000 Manual included in the
provided instruction manual folder.
•Heated Glass HG37:
As standard every MARS IVF workstation has a heated glass integrated in the table plate. The controller of the
heated glass has to be mounted and connected to the heated glass.
To Operate the Heated Glass refer to the HG37 included in the provided
instruction manual folder.

Version 4.2: Aug 26, 2015 19
•Humidifier:
The MARS IVF workstation is fitted with one humidifier with a single glass bottle as standard
Model variation:
- The MARS 1800 DUAL, has two humidifiers with a single glass bottle as standard each.
1. The nozzle for the output of gas is fitted on the back wall of the work area,
next to the humidifier glass bottle.
2. The glass bottle is placed in a aluminum block which is heated via the
table plate heating system
3. To use the humidifier, connect the gas tube located at the back of the
workstation to the laboratory premixed carbon dioxide gas source.
4. It is recommended that the gas inlet pressure to the workstation does not
exceed 0.5bar.
5. Fill the humidifier glass 4/5 up with distilled water. The water will only
need to be replaced occasionally.
Do not use 100% CO2; this will be lethal to your samples. Use only
premixed 5% or 6% CO2 gas concentration.
6. Use a glass hood (option, not provided as standard) and connect the
elbow with a silicon tube and connect the other end of the silicon tube to
the outlet of the gas located next to the humidifier glass bottle.
7. A standard setting on the gas valve is 4-5 l/hour, but this varies with the
user preferences.
8. Once satisfied with the gas flow into the glass hood, place it over the
specimens/petri dish on your heated table plate.
If not in use, remember to turn the gas valve to the minimum. For a
complete stop of the gas, the connection to the laboratory gas has to be
disconnected.
The glass bottle and the glass hoods can be disconnected and autoclaved.

20 Version 4.2: Aug 26, 2015
•LED / LCD Monitor:
The MARS IVF has an integrated monitor as standard. To adjust the contrast or the light intensity, follow the
instructions below.
1. The monitor comes integrated from the factory and is secured with a
frame and a cover glass.
2. Remove the frame around the monitor by unscrewing the screws.
Remove the frame and the cover glass.
3. Use a window suction tool (not provided) to pull the monitor out of the
back wall.
4. Place delicately the monitor on the table plate that has been covered to
avoid scratches. Adjust the contrast or brilliance of the monitor.
Place the monitor in the back wall and screw the frame with the cover
glass back in place.
Please refer to the manual of the monitor manufacturer for the operating
instructions of the monitor.
•Integrated microscope(s):
The stereomicroscope preparation is standard in the MARS IVF workstation.
The workstation will be delivered with a kit with different adaptors and bolts to fit most of the microscopes
This manual suits for next models
9
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