Leica DM5500 B User manual

1
Leica DM5500 B
Leica DM6000 B
Leica DM6000 M
Operating Manual
Bedienungsanleitung
Manuel d’utilisation

2
Published September 2007 by:
Herausgegeben September 2007 von:
Publié en septembre 2007 par :
Leica Microsystems CMS GmbH
Ernst-Leitz-Straße
D-35578 Wetzlar (Germany)
Responsible for contents:
Verantwortlich für den Inhalt:
Responsable du contenu :
Dr. Jasna Roeth, Stefan Motyka
(Product manager „Life Science Research“)
(Produktmanager „Life Science Research“)
(Chef de produit „Life Science Research“)
Holger Grasse
(Safety Officer according to MPG §30)
(Sicherheitsbeauftragter nach MPG §30)
(Officier de sécurité selon MPG §30)
In case of questions, please contact the hotline:
Bei Fragen wenden Sie sich bitte an die Hotline:
Pour toute question, contacter l’assistance en ligne: Phone [phone]
Fax [phone]
E-Mail: [email protected]

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Leica DM5500 B
Leica DM6000 B
Leica DM6000 M
Operating Manual

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Copyrights
Copyrights
All rights to this documentation are held by
Leica Microsystems CMS GmbH. Reproduction
of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method (in-
cluding electronic systems) is not allowed with-
out express written permission from Leica
Microsystems CMS GmbH.
The term "Windows" can be used in the follow-
ing text without further identification. It is a reg-
istered trademark of the Microsoft Corporation.
Otherwise, no inference with regard to the free
usability of product names may be drawn from
the use of those names.
The instructions contained in the following
documentation reflect state-of-the-art technol-
ogy and knowledge standards. We have com-
piled the texts and illustrations as accurately as
possible. Nevertheless, no liability of any kind
may be assumed for the accuracy of this manu-
al’s contents. Still, we are always grateful for
comments and suggestions regarding potential
mistakes within this documentation.
The information in this manual is subject to modifi-
cation at any time and without notification.

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Contents
7. Startup ......................................................... 39
7.1 Functional Principle .................................. 39
7.2 Switching on............................................... 46
7.3 The Leica SmartTouch .............................. 47
7.4 The Function Keys at the stand .............. 48
7.5 The Remote Control Element
SmartMove ................................................. 49
7.6 Köhler Illumination .................................... 49
7.6.1 Transmitted Light ............................ 49
7.6.2 Incident Light................................... 51
7.7 Checking Phase Contrast Rings ............. 52
7.8 Adjusting Motorized Polarizer ................ 54
7.9 Adjusting the Light Sources .................... 54
8. Operation .................................................... 60
8.1 Switching on............................................... 60
8.2 Stages and Specimen Displacement .... 62
8.2.1 Manual stage (DM5500 B) ............ 62
8.2.2 Motorized stage (DM6000 B/M)... 63
8.3 Focusing ...................................................... 64
8.4 Tubes ........................................................... 65
8.5 Eyepieces.................................................... 67
8.6 Objectives ................................................... 67
8.7 Magnification Changer............................. 70
8.8 Light Sources ............................................. 70
8.9 Aperture Diaphragm and
Field Diaphragm ......................................... 71
Contents
1. Important Notes about this Manual ...... 7
2. Intended Purpose of Microscopes ........ 8
3. Safety Notes ............................................... 9
3.1 General Safety Notes ............................... 9
3.2 Electrical Safety ........................................ 10
3.3 Disposal....................................................... 11
4. Overview of the Instrument .................... 12
5. Unpacking the Microscope .................... 18
6. Assembling the Microscope .................. 20
6.1 Stage ............................................................ 21
6.2 Condenser ................................................... 22
6.3 Tube and Eyepieces.................................. 23
6.4 Objectives ................................................... 24
6.5 Light Sources for the
Transmitted Light Axis.............................. 24
6.6 Light Sources for the
Incident Light Axis..................................... 26
6.6.1 106 z lamp housing ......................... 26
6.6.2 External light source EL6000 ........ 31
6.7 Equipping the
Incident Light Turret Disc ........................ 32
6.8 Polarizer and Analyzer ............................. 33
6.9 DIC Prisms .................................................. 34
6.10 Optional Accessories ............................... 35
6.11 Connecting the Leica CTR5500/CTR6000
Electronics Box.......................................... 37
6.12 Connecting the Computer ........................ 38
6.13 Connection to the Power Supply............ 38

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Contents
9. Contrasting Methods for
Leica DM5500 B and DM6000 B ............. 72
9.1 Transmitted Light....................................... 72
9.1.1 Bright Field ....................................... 72
9.1.2 Phase Contrast................................ 73
9.1.3 Dark Field ......................................... 73
9.1.4 Polarization ...................................... 74
9.1.5 Differential Interference Contrast .. 75
9.2 Fluorescence.............................................. 76
9.3 Combi Mode ............................................... 77
10. Contrasting Methods for
Leica DM6000 M ........................................ 78
10.1 Incident Light ............................................. 78
10.1.1Bright Field ....................................... 78
10.1.2Dark Field ......................................... 78
10.1.3Polarization ...................................... 79
10.1.4Interference Contrast .................... 80
10.1.5Fluorescence ................................... 80
10.2 Transmitted Light....................................... 81
10.2.1Bright Field ....................................... 81
10.2.2Polarization ...................................... 81
11. Trouble Shooting ....................................... 82
12. Care of the Microscope ........................... 85
12.1 Dust Cover .................................................. 85
12.2 Cleaning....................................................... 85
12.3 Handling Acids and Bases ...................... 86
13. Essential Wear and Spare Parts............ 87
14. Abbreviations and Pictograms .............. 88
15. Index ............................................................ 90
16. EU Declaration of Conformity................. 91

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1. Important Notes about this Manual
(1.2)
→ p.20
!
*
Numbers in parentheses, such as "(1.2)", corre-
spond to illustrations (in the example, Figure 1,
Item 2).
Numbers with pointer arrows (for example
→p.20), point to a certain page of this manual.
Caution!
Special safety instructions within this
manual are indicated with the triangle sym-
bol shown here, and have a gray back-
ground.
Caution! The microscope and accessories can
be damaged when operated incorrectly.
Notes on how to dispose of the microscope,
its components and expendables.
Explanatory note.
Item not contained in all configurations
(optional).
Text symbols, pictograms and their meanings:
Caution!
This operating manual is an essential com-
ponent of the microscope, and must be read
carefully before the microscope is assem-
bled, put into operation or used.
1. Important Notes about this Manual
This operating manual contains important in-
structions and information for the operational
safety and maintenance of the microscope and
accessories. Therefore, it must be kept and
taken care of.
For the operation of the LeicaScreen and the
Leica Application Suite (LAS), please see
separate operating manual.

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2. Intended Purpose of Microscopes
2. Intended Purpose of Microscopes
The Leica DM5500 B and DM6000 B micro-
scopes, to which this user manual belongs, are
designed for biological routine and research
applications. This includes the examination of
samples taken from the human body with a view
to providing information on physiological or
pathological states or congenital abnormalities,
or to determining the safety and compatibility
with potential recipients, or to monitoring thera-
peutic measures.
The Leica DM6000 M microscope is designed for
examinations in the field of materials research.
All the above-named microscopes comply with
the Council Directive 98/79/EEC concerning in
vitro diagnostics. They also conform to the
Council Directives 73/23/EEC concerning electri-
cal apparatus and 89/336/EEC concerning elec-
tromagnetic compatibility for use in an industrial
environment.
Caution!
The manufacturer assumes no liability for
damage caused by, or any risks arising from
using the microscopes for other purposes
than those for which they are intended or
not using them within the specifications of
Leica Microsystems CMS GmbH.
In such cases the conformity declaration
shall cease to be valid.
Caution!
These (IVD) devices are not intended for use
in the patient environment defined by DIN
VDE 0100-710. Neither are they intended for
combining with medical devices according
to EN 60601-1. If a microscope is electrically
connected to a medical device according to
EN 60601-1, the requirements defined in
EN 60601-1-1 shall apply.

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3. Safety Notes
3. Safety Notes
3.1 General Safety Notes
This safety class 1 device is constructed and
tested in accordance with
EN 61010-2-101:2002,
EN 61010-1:2001,
IEC 1010-1:2001,
safety regulations for electrical measuring, con-
trol, and laboratory devices.
Caution!
In order to maintain this condition and to en-
sure safe operation, the user must follow the
instructions and warnings contained in this
operating manual.
Caution!
The devices and accessories described in
this operating manual have been tested for
safety and potential hazards.
The responsible Leica affiliate or the main
plant in Wetzlar must be consulted when-
ever the device is altered, modified or used
in conjunction with non-Leica components
that are outside of the scope of this manual.
Unauthorized alterations to the device or
noncompliant use shall void all rights to any
warranty claims!

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3. Safety Notes
Leica EL6000
For indoor use only.
Supply voltage:
Frequency:
Power consumption:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Contamination class:
(see enclosed manual)
Caution!
The power plug may only be plugged into an
outlet equipped with a grounding contact.
Do not interfere with the grounding function
by using an extension cord without a ground
wire. Any interruption of the ground wire in-
side or outside of the device, or release of
the ground wire connection, can cause the
device to become hazardous. Intentional
ground interruption is not permitted!
3.2 Electrical Safety
General specifications
Leica CTR5500 and CTR6000 electronics box
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
Microscope
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
Supply unit ebq 100*
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
(see enclosed manual)
90-250 V~
50-60 Hz
max. 290 VA
T6,3 A
(IEC 60127-2/3)
15-35°C
max. 80% to 30°C
II
2
90-250 V~
50-60 Hz
See CTR5500/6000
See CTR5500/6000
15-35°C
max. 80% to 30°C
II
2
90-250 V~
50-60 Hz
max. 155 VA
2xT2A (IEC 127)
10-36°C
max. 80% to 30°C
II
2
100-240 V~ (±10%)
50-60 Hz
max. 210 VA
5x20, 2.5 A, slow,
breaking capacity H
→EL6000 manual
0°-40°C
10-90%
non-condensing
II
2

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3. Safety Notes
Caution!
Through activating to the grounding connec-
tion (earth screw on the back of the Leica
CTR5500 and CTR6000 Electronics Box)
ancillary equipment with its own and/or
extra power supply may be brought to the
same ground wire potential. For connections
without a ground connector, Leica Service
must be consulted.
Caution!
Never use any fuses as replacements other
than those of the types and the current rat-
ings listed here. Using patched fuses or
bridging the fuse holder is not permitted.
Caution!
The microscope’s electrical accessory com-
ponents are not protected against water.
Water can cause electric shock.
Caution!
Protect the microscope from excessive tem-
perature fluctuations. Such fluctuations can
lead to the accumulation of condensation,
which can damage the electrical and optical
components.
Ambient temperature: 15-35°C.
Caution!
Before exchanging the fuses or lamps, be
absolutely certain to switch-off the main
power switch and remove the power cable.
Caution!
Touch the touchscreen using your finger
only. Never use a pen or other hard, sharp or
pointed objects.
3.3 Disposal
After the end of the product’s life, please
contact Leica Service or Leica Sales on how to
dispose of it.
Please observe the national laws and
ordinances which, for example, implement and
ensure compliance with EU directive WEEE.
n.b.:
Like all electronic instruments, the
microscope, its components and expend-
ables may not be disposed of as general
household waste!

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4. Overview of the Instrument
4. Overview of the Instrument
Specification
Contrasting Method
Transmitted Light Axis
Incident Light Axis
Tube
Leica DM5500 B/DM6000 B
• transmitted light:
BF, DF, PH, ICT (DIC), Pol
• incident light: Fluo
• integrated into the stand
• motorized 8x filter turret
( 5x optional)
• with FIM (Fluorescence In-
tensity Manager) for de-
creasing light intensity in 5
steps
• mechanical “Booster Lens“
for increasing fluorescence
intensity
• motorized Excitation Man-
ager to control the fluores-
cence emission when using
double and triple filter cubes
(optional).
• ultra-fast, internal filter
wheel (IFW), motorized
(optional)
• motorized shutter
(speed < 0.1 s)
Leica DM6000 M
• transmitted light: BF, Pol
• incident light:
BF, DF, ICR (DIC), Pol, Fluo
• integrated into the stand
• motorized 4x filter turret
(2 fixed positions, 2 variable
positions)
• automatic Illumination
Manager
• motorized shutter
(speed < 0.1 s)
• automatic Illumination Manager
(automatic aperture diaphragm and field diaphragm,
automatic intensity control)
• automatic Constant Color Intensity Control (CCIC)
• motorized shutter
• motorized or manual
• up to 3 beam splitting positions
• optionally with two camera outputs
(for one digital and e.g. one analog camera)

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4. Overview of the Instrument
Specification
Magnification Changer
(optional)
Objective Turret
X/Y Stage
Condenser
Z Drive
Leica DM5500 B/DM6000 B
• manual
• absolute coded
• magnification steps:
1x; 1.25x; 1.6x
• DM6000 B: motorized
DM5500 B: manual
• absolute coded
• 7x for objectives with M25
thread
• mot. DIC objective prism tur-
ret with 4 positions
(optional)
Leica DM6000 M
• manual
• absolute coded
• magnification steps:
1x; 1.5x; 2x
• motorized
• absolute coded
• 6x for objectives with M32
thread
• mot. DIC objective prism
turret with 4 positions
(optional)
• motorized condenser head
• automatic change between contrasting methods:
motorized condenser turret for light rings, DF stop,
DIC prisms
• optional polarizer integrated and motorized
• automatic Köhler Illumination
• motorized
• dovetail for exchanging stage
• travel range: 25 mm
• smallest increment: 0.015µm
• max. speed: 5 mm/s
• min. speed: 1 mm/s
• max. load: 4 kg
• DM6000 B/M: motorized, direct stepper motor drive
DM5500 B: manual
• replaceable specimen stage
• zero position defined by end switch
• outer dimensions: 234 mm x 157 mm
• travel range: 76 mm x 50 mm
• smallest increment: 0.3 µm

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4. Overview of the Instrument
Specification
Control Panels
Leica CTR5500
Leica CTR6000
Electronics Box
Computer Interface
Software Tools
Leica DM5500 B/DM6000 B Leica DM6000 M
• USB 2.0
• Leica Application Suite (LAS)
for WindowsTM 2000, XP, Vista
• for:
• microscope and camera configuration
• microscope and camera control
• image recording and image storing
• separate control unit to control
• z-drive
• xy-stage (CTR6000)
• objective turret (CTR6000)
with:
• supply voltage for 100W halogen lamp
• supply voltage for SmartMove
See →p.10 (electrical safety))
• Function keys at the stand
• additional variable function keys
• focusing knobs
• Leica SmartTouch: touch sensitive LC display
• SmartMove: ergonomic control element
for x,y,z control with additional variable function keys
• Leica STP6000: ergonomic control element for x,y,z
control with 11 additional variable function keys
and touch sensitive LC display

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4. Overview of the Instrument
Fig. 1 System overview DM6000 B
1Touchscreen
2Information key
3,4 Variable function keys, user-programmable
5Fine focus adjustment
6Coarse focus adjustment
7Movement in Y direction
8Movement in X direction
Abb. 1 Control elements of the Leica STP6000
2
356 7
81
4

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4. Overview of the Instrument
1Eyepiece
2Eyepiece tube
3Motorized tube MBDT
4Motorized objective nosepiece with objectives
5Motorized specimen stage with specimen holder
6Condenser
7Leica SmartTouch
8Function keys field diaphragm
9Transmitted light/incident light switch
10 Function keys aperture diaphragm
11 Function keys light intensity
12 Focus wheel
13 Variable function keys (factory pre-assigned)
14 Lamp adjustment window
Fig. 2 Left side of the stand Leica DM6000 B
n.b.: Illustrations for Leica DM5500 B similar, but with manual objective nosepiece and manual
specimen stage
1
2
3
4
5
6
7
9101112
13
14

17
4. Overview of the Instrument
15 Lamp housing for incident light
16 Lamp housing for transmitted light
17 Transmitted light filter, optional
18 Transmitted light filter, optional
19 Variable function keys (factory pre-assigned)
20 Focus wheel
21 DIC turret
22 Motorized filter turret
23 Magnification changer
Fig. 3 Right side of the stand Leica DM6000 B Fig. 4 Remote control element SmartMove
1Movement (x-direction)
2Movement (y-direction)
3Focus adjustment
4Variable function keys
(factory pre-assigned)
1
2
3
4
23
20 19 18 17
15
16
22
21

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5. Unpacking the Microscope
The device is delivered in several boxes.
The stand box contains the following compo-
nents:
• Stand with integrated incident light axis and
objective nosepiece
• Specimen stage with stage bracket
• Power cable and PC connecting cable
• CD with software package Leica Application
Suite (LAS)
• Instructions and list of microscope default
settings („Identification Sheet“)
The system box contains the microscope acces-
sories:
• Tube
• Eyepieces
• Objectives
• Condenser
• Lamp housings with accessories
• Fitting tool
• Depending on configuration, additional micro-
scope accessories such as filter cubes, etc.
The Leica CTR5500 or CTR6000 Electronics Box,
the remote control element SmartMove, the
external ebq 100 supply unit* and the external
EL6000 compact light source* are delivered in
separate packaging.
First, carefully remove all components from the
transportation and packaging materials.
Note:
If at all possible, avoid touching the lens sur-
faces of the objectives. If fingerprints do appear
on the glass surfaces, remove them with a soft
leather or linen cloth. Even small traces of finger
perspiration can damage the surfaces in a short
time. See the chapter, "Care of the Microscope"
→
p. 85, for additional instructions.
Caution!
Do not yet connect the microscope and pe-
ripherals to the power supply at this point!
5. Unpacking the Microscope

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5. Unpacking the Microscope
Installation location
Work with the microscope should be performed
in a dust-free room, which is free of oil vapors
and other chemical vapors, as well as extreme
humidity. At the workplace, large temperature
fluctuations, direct sunlight and vibrations
should be avoided. These conditions can distort
measurements and micrographic images.
Allowable ambient conditions
Temperature 15-35°C
Relative humidity maximum 80% up to 30°C
Microscopes in warm and warm-damp climatic
zones require special care in order to prevent
the build up of fungus.
See the chapter, "Care of the Microscope" →
p. 85,
for additional instructions.
Caution!
Electrical components must be placed at least
10 cm from the wall and away from flamma-
ble substances.
Transport
For shipping or transporting the microscope
and its accessory components, the original
packaging should be used.
As a precaution to prevent damage from vibra-
tions, the following components should be dis-
assembled and packaged separately:
• Unscrew the objectives.
• Remove the condenser.
• Remove the stage.
• Remove the lamp housings.
• Disassemble the burner of 106 z lamp housing.
• Remove all moving or loose parts.

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6. Assembly
6. Assembling the Microscope
The microscope components are logically as-
sembled in this order:
• Stage
• Condenser
• Tube
• Eyepieces
• Objectives
• Light sources
• Filter cubes/reflectors*
Only a few commonly used screwdrivers and
keys are necessary for assembly, which are in-
cluded in the delivery package.
When using intermediate systems and optical
accessories, the sequence may vary.
In this case, read Chapter
"6.10 Optional accessories" →p. 35.