Leica DM5500 B User manual

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Leica DM5500 B
Leica DM6000 B
Leica DM6000 M
Operating Manual
Bedienungsanleitung
Manuel d’utilisation

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Published September 2007 by:
Herausgegeben September 2007 von:
Publié en septembre 2007 par :
Leica Microsystems CMS GmbH
Ernst-Leitz-Straße
D-35578 Wetzlar (Germany)
Responsible for contents:
Verantwortlich für den Inhalt:
Responsable du contenu :
Dr. Jasna Roeth, Stefan Motyka
(Product manager „Life Science Research“)
(Produktmanager „Life Science Research“)
(Chef de produit „Life Science Research“)
Holger Grasse
(Safety Officer according to MPG §30)
(Sicherheitsbeauftragter nach MPG §30)
(Officier de sécurité selon MPG §30)
In case of questions, please contact the hotline:
Bei Fragen wenden Sie sich bitte an die Hotline:
Pour toute question, contacter l’assistance en ligne: Phone [phone]
Fax [phone]
E-Mail: [email protected]

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Leica DM5500 B
Leica DM6000 B
Leica DM6000 M
Operating Manual

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Copyrights
Copyrights
All rights to this documentation are held by
Leica Microsystems CMS GmbH. Reproduction
of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method (in-
cluding electronic systems) is not allowed with-
out express written permission from Leica
Microsystems CMS GmbH.
The term "Windows" can be used in the follow-
ing text without further identification. It is a reg-
istered trademark of the Microsoft Corporation.
Otherwise, no inference with regard to the free
usability of product names may be drawn from
the use of those names.
The instructions contained in the following
documentation reflect state-of-the-art technol-
ogy and knowledge standards. We have com-
piled the texts and illustrations as accurately as
possible. Nevertheless, no liability of any kind
may be assumed for the accuracy of this manu-
al’s contents. Still, we are always grateful for
comments and suggestions regarding potential
mistakes within this documentation.
The information in this manual is subject to modifi-
cation at any time and without notification.

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Contents
7. Startup ......................................................... 39
7.1 Functional Principle .................................. 39
7.2 Switching on............................................... 46
7.3 The Leica SmartTouch .............................. 47
7.4 The Function Keys at the stand .............. 48
7.5 The Remote Control Element
SmartMove ................................................. 49
7.6 Köhler Illumination .................................... 49
7.6.1 Transmitted Light ............................ 49
7.6.2 Incident Light................................... 51
7.7 Checking Phase Contrast Rings ............. 52
7.8 Adjusting Motorized Polarizer ................ 54
7.9 Adjusting the Light Sources .................... 54
8. Operation .................................................... 60
8.1 Switching on............................................... 60
8.2 Stages and Specimen Displacement .... 62
8.2.1 Manual stage (DM5500 B) ............ 62
8.2.2 Motorized stage (DM6000 B/M)... 63
8.3 Focusing ...................................................... 64
8.4 Tubes ........................................................... 65
8.5 Eyepieces.................................................... 67
8.6 Objectives ................................................... 67
8.7 Magnification Changer............................. 70
8.8 Light Sources ............................................. 70
8.9 Aperture Diaphragm and
Field Diaphragm ......................................... 71
Contents
1. Important Notes about this Manual ...... 7
2. Intended Purpose of Microscopes ........ 8
3. Safety Notes ............................................... 9
3.1 General Safety Notes ............................... 9
3.2 Electrical Safety ........................................ 10
3.3 Disposal....................................................... 11
4. Overview of the Instrument .................... 12
5. Unpacking the Microscope .................... 18
6. Assembling the Microscope .................. 20
6.1 Stage ............................................................ 21
6.2 Condenser ................................................... 22
6.3 Tube and Eyepieces.................................. 23
6.4 Objectives ................................................... 24
6.5 Light Sources for the
Transmitted Light Axis.............................. 24
6.6 Light Sources for the
Incident Light Axis..................................... 26
6.6.1 106 z lamp housing ......................... 26
6.6.2 External light source EL6000 ........ 31
6.7 Equipping the
Incident Light Turret Disc ........................ 32
6.8 Polarizer and Analyzer ............................. 33
6.9 DIC Prisms .................................................. 34
6.10 Optional Accessories ............................... 35
6.11 Connecting the Leica CTR5500/CTR6000
Electronics Box.......................................... 37
6.12 Connecting the Computer ........................ 38
6.13 Connection to the Power Supply............ 38

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Contents
9. Contrasting Methods for
Leica DM5500 B and DM6000 B ............. 72
9.1 Transmitted Light....................................... 72
9.1.1 Bright Field ....................................... 72
9.1.2 Phase Contrast................................ 73
9.1.3 Dark Field ......................................... 73
9.1.4 Polarization ...................................... 74
9.1.5 Differential Interference Contrast .. 75
9.2 Fluorescence.............................................. 76
9.3 Combi Mode ............................................... 77
10. Contrasting Methods for
Leica DM6000 M ........................................ 78
10.1 Incident Light ............................................. 78
10.1.1Bright Field ....................................... 78
10.1.2Dark Field ......................................... 78
10.1.3Polarization ...................................... 79
10.1.4Interference Contrast .................... 80
10.1.5Fluorescence ................................... 80
10.2 Transmitted Light....................................... 81
10.2.1Bright Field ....................................... 81
10.2.2Polarization ...................................... 81
11. Trouble Shooting ....................................... 82
12. Care of the Microscope ........................... 85
12.1 Dust Cover .................................................. 85
12.2 Cleaning....................................................... 85
12.3 Handling Acids and Bases ...................... 86
13. Essential Wear and Spare Parts............ 87
14. Abbreviations and Pictograms .............. 88
15. Index ............................................................ 90
16. EU Declaration of Conformity................. 91

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1. Important Notes about this Manual
(1.2)
→ p.20
!
*
Numbers in parentheses, such as "(1.2)", corre-
spond to illustrations (in the example, Figure 1,
Item 2).
Numbers with pointer arrows (for example
→p.20), point to a certain page of this manual.
Caution!
Special safety instructions within this
manual are indicated with the triangle sym-
bol shown here, and have a gray back-
ground.
Caution! The microscope and accessories can
be damaged when operated incorrectly.
Notes on how to dispose of the microscope,
its components and expendables.
Explanatory note.
Item not contained in all configurations
(optional).
Text symbols, pictograms and their meanings:
Caution!
This operating manual is an essential com-
ponent of the microscope, and must be read
carefully before the microscope is assem-
bled, put into operation or used.
1. Important Notes about this Manual
This operating manual contains important in-
structions and information for the operational
safety and maintenance of the microscope and
accessories. Therefore, it must be kept and
taken care of.
For the operation of the LeicaScreen and the
Leica Application Suite (LAS), please see
separate operating manual.

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2. Intended Purpose of Microscopes
2. Intended Purpose of Microscopes
The Leica DM5500 B and DM6000 B micro-
scopes, to which this user manual belongs, are
designed for biological routine and research
applications. This includes the examination of
samples taken from the human body with a view
to providing information on physiological or
pathological states or congenital abnormalities,
or to determining the safety and compatibility
with potential recipients, or to monitoring thera-
peutic measures.
The Leica DM6000 M microscope is designed for
examinations in the field of materials research.
All the above-named microscopes comply with
the Council Directive 98/79/EEC concerning in
vitro diagnostics. They also conform to the
Council Directives 73/23/EEC concerning electri-
cal apparatus and 89/336/EEC concerning elec-
tromagnetic compatibility for use in an industrial
environment.
Caution!
The manufacturer assumes no liability for
damage caused by, or any risks arising from
using the microscopes for other purposes
than those for which they are intended or
not using them within the specifications of
Leica Microsystems CMS GmbH.
In such cases the conformity declaration
shall cease to be valid.
Caution!
These (IVD) devices are not intended for use
in the patient environment defined by DIN
VDE 0100-710. Neither are they intended for
combining with medical devices according
to EN 60601-1. If a microscope is electrically
connected to a medical device according to
EN 60601-1, the requirements defined in
EN 60601-1-1 shall apply.

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3. Safety Notes
3. Safety Notes
3.1 General Safety Notes
This safety class 1 device is constructed and
tested in accordance with
EN 61010-2-101:2002,
EN 61010-1:2001,
IEC 1010-1:2001,
safety regulations for electrical measuring, con-
trol, and laboratory devices.
Caution!
In order to maintain this condition and to en-
sure safe operation, the user must follow the
instructions and warnings contained in this
operating manual.
Caution!
The devices and accessories described in
this operating manual have been tested for
safety and potential hazards.
The responsible Leica affiliate or the main
plant in Wetzlar must be consulted when-
ever the device is altered, modified or used
in conjunction with non-Leica components
that are outside of the scope of this manual.
Unauthorized alterations to the device or
noncompliant use shall void all rights to any
warranty claims!

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3. Safety Notes
Leica EL6000
For indoor use only.
Supply voltage:
Frequency:
Power consumption:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Contamination class:
(see enclosed manual)
Caution!
The power plug may only be plugged into an
outlet equipped with a grounding contact.
Do not interfere with the grounding function
by using an extension cord without a ground
wire. Any interruption of the ground wire in-
side or outside of the device, or release of
the ground wire connection, can cause the
device to become hazardous. Intentional
ground interruption is not permitted!
3.2 Electrical Safety
General specifications
Leica CTR5500 and CTR6000 electronics box
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
Microscope
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
Supply unit ebq 100*
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
(see enclosed manual)
90-250 V~
50-60 Hz
max. 290 VA
T6,3 A
(IEC 60127-2/3)
15-35°C
max. 80% to 30°C
II
2
90-250 V~
50-60 Hz
See CTR5500/6000
See CTR5500/6000
15-35°C
max. 80% to 30°C
II
2
90-250 V~
50-60 Hz
max. 155 VA
2xT2A (IEC 127)
10-36°C
max. 80% to 30°C
II
2
100-240 V~ (±10%)
50-60 Hz
max. 210 VA
5x20, 2.5 A, slow,
breaking capacity H
→EL6000 manual
0°-40°C
10-90%
non-condensing
II
2