Optika Italy B-1000 Series User manual

Model
B-1000
B-1000BF
B-1000PH
B-1000TI-2
B-1000TI-3
B-1000TI-5
B-1000TI-10
B-1000 Series
INSTRUCTION MANUAL
Ver. 3.1 2019

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 Manual version 4
5.2 Motorised version 6
5.3 B-1000TI-2 / 3 / 5 / 10 8
6. Unpacking 9
7. Assembling 9
7.1 Manual version 9
7.2 Motorised version 10
7.3 B-1000TI-2/3/5/10 11
7.4 Assembling the microscope 12
7.4.1 Manual version 12
7.4.2 Motorised version 14
7.4.3 B-1000TI-2 / 3 / 5 / 10 15
8. Summaryofbrighteldobservationprocedures 18
9. Use of the microscope 19
9.1 General switch on 19
9.2 Control keyboard 19
9.3 Brightness adjustment 19
9.4 Adjust the observation head 20
9.5 Adjust the interpupillary distance 20
9.6 Diopter adjustment 20
9.7 Use of eyeshields 20
9.8 Light path selection 21
9.9 Coarse focus tension adjustment 21
9.10 Focus stop lever 22
9.11 Stage 22
9.12 Centering the condenser 23
9.13 Effectofelddiaphragm 23
9.14 Aperture diaphragm 23
9.15 Use of oil immersion objective 24
9.16 Use of ALC system (optional) 24
9.17 Only for motorised version 25
9.17.1 Nosepiece rotation 25
9.17.2 Focusing 25
9.17.3 Stage 25
9.18 Use of the pointer (B-1000TI-2 / 3 / 5 / 10) 25
10. UseofuniversalcondenserforBrighteld/Darkeld/Phasecontrast 27
10.1 Brighteldobservation(BF) 27
10.2 Darkeldobservation(DF) 27
10.3 PhaseContrastobservation(PH) 28
10.4 Useofgreenlter 29
11. DIC observation 30
11.1 Koehler DIC transmitted light 30
11.2 Nomarski DIC transmitted light 31
12. Microphotography 33
12.1 Use of C-mount cameras 33
12.2 Useofreexcamera 33
13. Maintenance 34
14. Troubleshooting 35
Equipment disposal 37
Summary

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICALSHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
EYEPIECE
PHOTO / TV
PORT
OBSERVATION
HEAD
ALC SYSTEM
DIOPTER
ADJUSTMENT
RING
DIC PRISM
SLOT
CONTROL
KEYBOARD
MAIN
SWITCH
FOCUS LOCK
LEVER INTENSITY
ADJUSTMENT
DIAL
FIELD
DIAPHRAGM
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
5.1 Manual version

Page 5
FILTER
HOLDER
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
TENSION
ADJUSTMENT
RING
APERTURE
DIAPHRAGM
LIGHT PATH
SELECTOR
LEVER
STAGE
SLIDE
HOLDER
X-Y STAGE
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
ALC SOCKET
Opposite side

Page 6
5.2 Motorised version
Only the parts related to the motorised version are highlighted.
NOSEPIECE
FOCUS
MOTOR
X-AXIS MOVEMENT
KNOB
(MANUAL MOVEMENT)
STAGE

Page 7
STAGE
CONNECTION
CABLE
NOSEPIECE
ROTATION
BUTTONS
Y-AXIS MOVEMENT
KNOB
(MANUAL MOVEMENT)
Opposite side

Page 8
5.3 B-1000TI-2 / 3 / 5 / 10
Only the multi-head parts are highlighted.
POINTER BRIGHTNESS
ADJUSTMENT / POINTER
MOVEMENT
SIDE
OBSERVATION
HEADS
POINTER COLOR
SELECTOR

Page 9
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
7.1 Manual version
① Frame
② Objectives
③ Stage
④ Condenser
⑤ Observation head
⑥ Eyepieces
⑦ ALC system (M-1030) (Optional)
⑧ Power supply
⑨ Dust cover
⑩ Allen wrench
⑪ Immersion oil
③
④
⑤
⑥
⑦
⑧
①
②
⑪
⑩
⑨

Page 10
⑪⑩
⑨
① Frame
② Objectives
③ Stage
④ Condenser
⑤ Observation head
⑥ Eyepieces
⑦ ALC system (M-1030) (Optional)
⑧ Microscope power supply
⑨ Motorised parts power supply
⑩ Serial cable
⑪ PS/2 mouse
⑫ Dust cover
⑬ Allen wrench
⑭ Immersion oil
①②
③
④
⑤
⑥
⑦
⑧
7.2 Motorised version
⑫⑬⑭

Page 11
7.3 B-1000TI-2/3/5/10
① Frame
② Objectives
③ Stage
④ Condenser
⑤ Main observation head
⑥ Side observation heads
• one for B-1000TI-2
• two for B-1000TI-3
• four for B-1000TI-5
• nine for B-1000TI-10
⑦ Eyepieces
• 10x/22 (one pair for main head)
• 10x/20 (one pair for B-1000TI-2)
• 10x/20 (two pairs for B-1000TI-3)
• 10x/20 (four pairs for B-1000TI-5)
• 10x/20 (nine pairs for B-1000TI-10)
⑧ Power supply
• one for microscope (6V dc)
• one for multi-head attachment (5Vdc)
⑨ Dust cover
⑩ Allen wrench
⑪ Immersion oil
①
②
③
④
⑤
⑥
⑦
⑪
⑨⑩⑧

Page 12
7.4 Assembling the microscope
7.4.1 Manual version
1. Put the microscope stand on a solid table. Insert
M-1030 attachment (if provided) above the stand,
using the 2mm Allen wrench to tighten the screw.
(Fig. 1)
2. Connect the cable of the ALC (Automatic Light
Control) system to the socket placed on the right
side of the frame. (Fig. 2)
3. Insert the optical head above the attachment,
using the 2mm Allen wrench to tighten the screw.
(Fig. 3)
4. Insert eyepieces into the empty eyepiece slee-
ves. (Fig. 4)
Fig. 1
Fig. 2
Fig. 4
Fig. 3

Page 13
5. Insert the condenser under the stage: position
until it is well inserted into its holder (under the
condenser there is a pin that must fully enter the
holder guide). (Fig. 5)
6. Lock the condenser xing knob ①.
7. Mount the stage: lower the support using the co-
arse focus knob, then place the stage and rmly
tighten the lock screw ②. (Fig. 6)
8. Screw each objective into the thread of the no-
sepiece, clockwise with increasing magnication.
(Fig. 7)
9. Insert the power supply jack in the socket placed
in the rear side of the of the microscope. (Fig. 8)
①
Fig. 5
Fig. 6
Fig. 8
Fig. 7
②

Page 14
7.4.2 Motorised version
1. Assemble the stage in the same way as the ma-
nual version. Check the perfect alignment of the
rear part of the stage with the rear arm of the fra-
me. An imperfect alignment could lead to an in-
correct functioning of the system. (Fig. 9)
2. Connect the cable ① from the stage to the frame
and tight the locking screws of the connectors ②.
(Fig. 10)
3. Connect the provided cables: ③ 12V power sup-
ply for the motorised parts; ④6V microscope po-
wer supply; ⑤ serial cable; ⑥ PS/2 mouse. Con-
nect power cables as the last step. (Fig. 11)
Fig. 9
Fig. 10
Fig. 11
②
①
③ ④ ⑤⑥

Page 15
7.4.3 B-1000TI-2 / 3 / 5 / 10
1. Place the splitter attachment of the multidiscussion
system and tighten the lock screw ① on the right
side of the frame. (Fig. 12)
2. Connect the 5Vdc power supply to the rear socket
of the splitter attachment. (Fig. 6).
3. Connect the rst part of the extension tube to the
optical splitter. Insert the tube into the splitter all
the way down and screw on the black sealing ring
completely. (Fig. 14-15).
• Each connection is labelled with a letter
printed on both sides of the connection. Make
sure to match the letters in order to correctly
assemble the microscope.
①
Fig. 12
Fig. 13
Fig. 15
Fig. 14
①

Page 16
4. Insert the second part of the extension tube. (Fig.
16).
5. Fully insert the second extension tube in the
right position. Using the provided Allen wrench
(small one) lock the xing screws ① to block the
extension tube.
• Attheendoftherstextensiontubethereis
a lens (Fig. 17). Make sure it is free from dirt,
dust or other contaminants before to proceed
withtheassemblingofthesecondextension
tube.
6. Adjust the height of the multi-head holder. Loosen
the base xing knob ②, unscrew the base ③in
order to reach the desired height, then lock the
knob. (Fig. 18). Make sure that each extension
tube is perfectly horizontal.
7. Insert the binocular heads, matching the reference
letter. (Fig. 19).
Fig. 16
①
②
③
Fig. 17
Fig. 19
Fig. 18

Page 17
8. Insert the provided eyepieces (WF10X/20) into
binocular heads. (Fig. 20)
9. Repeat all the above operations for each
observation point.
10. Install the trinocular head over the splitter. (Fig.
21)
11. Continue with the installation of all other
components as described in the paragraph 7.4.1.
①
Fig. 20
Fig. 21

Page 18
Main switch
(Used commands) (Chapter)
Slide holder
Revolving nosepiece
Observation head
Diopter adjustment ring
Condenser centering
Aperture diaphragm
Field diaphragm
Revolving nosepiece
Light adjustment dial
Coarse and fine focusing knobs
Coarse and fine focusing knobs
Bring on “1” the main switch
Put a specimen on the stage
Insert 10X objective into the light path
Focus the specimen
Begin observation
Adjust field and aperture iris diaphragms
Insert desired objective for the observation and focus the
specimen
Adjust interpupillary distance
Adjust diopters
Center optical axis
Adjust light intensity
9
9
5
5
5
5
9
9
9
9
9
9
ON-OFF key on the keyboard
Light adjustment dial
Turn on the microscope and adjust light intensity 9
9
ALC system 9
8. Summaryofbrighteldobservationprocedures

Page 19
9.1 General switch on
To activate the transmitted light illuminator, put the
main switch ①, located on the left side of the stand,
to the position “1”. (Fig. 22)
9.2 Control keyboard
B-1000 illumination can be managed through the
keyboard placed on the left of the stand. (Fig. 23)
• ON-OFF (②): press this key (after switching the
main switch on “1”) to turn ON or OFF the micro-
scope LED.
• BOOST (③): press this button in order to in-
crease the brightness (useful for high-magni-
cation objectives or very opaque specimens).
Do not enable boost mode while observing
with low magnication objectives (4x, 10x)
with fully open diaphragm: the high bright-
ness may hurt user’s eyes.
• AUTO OFF (④): if you want the illuminator to
switch off automatically, press this button until 15,
30 or 60 minutes delay is set. After this period of
time, the light will turn off. You have to press the
ON-OFF button to turn it on again.
9.3 Brightness adjustment
Use the brightness adjustment dial ⑤ on the left side
of the microscope to increase or decrease the light
intensity on the specimen. (Fig. 24)
9. Use of the microscope
③
④
②
Fig. 23
Fig. 24
⑤
①
Fig. 22
①

Page 20
9.4 Adjust the observation head
Loosen the locking screw ①, turn the observation
head to a comfortable position for observation, and
then lock the locking screw again. (Fig. 25)
9.5 Adjust the interpupillary distance
Observing with both eyes, hold the two eyepiece
prism assemblies. Rotate them around their common
axis until the elds of view coincide.
• The graduation on the interpupillary distance
indicator ②, pointed by the spot “.” on the
eyepiece holder, shows the distance between
the operator’s eyes. (Fig. 26)
The range of the interpupillary distance is 48-75 mm.
9.6 Diopter adjustment
1. Look into the right eyepiece with your right eye
only, and focus on the specimen.
2. Look into the left eyepiece with your left eye
only. If the image is not sharp, use the dioptric
adjustment ring ③ to compensate. (Fig. 27)
• The adjustment range is ±5 diopter. The
number indicated on the adjustment ring
graduation should correspond to the
operator’s dioptric correction.
9.7 Use of eyeshields
• Use with eyeglasses
Fold rubber eyeshields with both hands. Folded
eyeshields avoid scratching the lenses of eyeglas-
ses. (Fig. 28)
Fig. 25
Fig. 26
Fig. 28
Fig. 27
①
③
②