Rayto RT-7200 User manual

RT-7200
Auto Hematology Analyzer
User’s Manual
Copyright: Rayto Life and Analytical Sciences Co., Ltd.
Address: C&D/4F, 7th Xinghua Industrial Bldg, Nanhai Rd, Nanshan,
Shenzhen 518067, P.R. China
Rev: 1.0e

RT-7200 Auto Hematology Analyzer User’s Manual
How to use this manual
Thank you very much for being the user of RT-7200 Auto Hematology Analyzer.
In order to get best test result, before doing clinical test, you shall be familiar with this
analyzer and its performance. This user’s manual is the using guidline of RT-7200 Auto
Hematology Analyzer from Rayto, including installation, daily test, QC and daily
maintenance.
The functions of instruments with difference versions or configurations may be
different. The content of user’s manual may be different because of upgrade, we will
not give notice seperately. If you have any question, please contact the distributor.
Please reserve all packing materials for storage, transportation and return to fctory for
maitenance.
If any problems come into existence, please contact the distributors
N
No ot te e Prompts, advice or suggestion, using italic.
W
Wa ar rn ni in ng g Warnings must be followed strictly to make sure the normal
performance of instrument and the accuracy of test result.
Declaration
Rayto reserves the Power of Interpretation to this user’s manual.
The figures in the manual is only for demostration, they may be different form the actual
display, please according to the actual display. Unless been authorised by Rayto, No person
or orgnization has the right to copy, modify or translate this user’s manual.
Only that all the following requirements are met, Rayto will be responsible for the safety,
reliability and performance of the product.
zInstall, adjust, update and repaire are done by Rayto authorised person.
zOperation of instrument followed the user’s manual strictly.
zElectric environmentmeet the requirements.
Note
zThis instrument shall be operated by prefessional of medical inspection or trained
doctor, nurse and experimentalist.
Warning
zIf the orgnization which are using this instrument can not finish a satisfactory
maintenance/ repair plan for the instrument, it may cause abnomal fault to the anlyzer
even endanger the health of operator.
zThe analyzer must be operated under the stated conditions, if not, it may cause
abnormal running of instrument, the test results may be not reliable, also it can break
the parts of analyzer even endanger the health of operator.
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RT-7200 Auto Hematology Analyzer User’s Manual
Main graphical representations used on this instrument
Temperature limitation
Fragile articles
The fragile articles are contained in the transport package, so it
should be handled with care when being transported
Upwards
It indicates that the transport package shall be kept vertically
upward
To be protected from rain
The packaging piece is extensive to the rain
Limit of the number of stacking tiers
It indicates the maximum number of the stacking tiers of the
same package. 3 indicate the limit of the stacking tiers.
Biological risks
This means that the labeled item could lead to personal
injury and/or damage to the analyzer. The symbol is labeled
beside the power outlet and some external interface.
Serial number
In vitro diagnostic medical device.
Manufacturer
Authorised representative in the European community
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RT-7200 Auto Hematology Analyzer User’s Manual
Warnings and security attentions
This instrument is only provided for in vitro diagnosis, please carefully read the following
warnings before use. They are required to be strictly followed.
Warning: please carefully read the following security attentions before use this
instrument.
zIf the smoke, peculiar smell and sound appears while using, the power supply
must be immediately cut off. Meanwhile, the inspection apply shall be
immediately presented towards the distributors or agents of Rayto company. If
the instrument continues to be used under this circumstance, the misfire, electric
shock or casualties of personnel might be caused.
zThe bloods, reagents and metal pieces shall be avoided from entering the interior
of this instrument, or the short circuit or misfire with smoke generation will be
caused. If abnormality happens, the power supply shall be immediately cut off
and the plug of the power supply is extracted from the power socket. Meanwhile,
the inspection apply shall be immediately presented towards the distributors and
the agents of this company.
zThe operator shall not touch the electronic lines in the instrument; especially
there will be a danger of electric shock when they are touched by a wet hand.
zThe rubber gloves are required to be worn and the prescriptive tools and parts
must be used when the instrument is maintained and checked. When the
operation ends, please wash your hands using disinfection solutions, or the
infection, electric shock or scald might be caused to the part of the skin which
contacts with the bloods.
zWhen the samples are handled, the great care shall be paid and the rubber gloves
must be worn, or the infections might be caused. If the samples enter the eyes or
wounds, they shall be immediately washed using a large amount of clear water
and inspected by the doctor.
Usage of reagents
zThe reagents shall be avoided from contacting with the skins and clothes when
the operation is carried out.
zIf the reagents enter the eyes immodestly, they should be immediately washed to
be clean using a large amount of clear water and inspected by the doctor.
zIf the reagents are wrongly drunk, the help shall be immediately obtained from the
doctors and simultaneously a large amount of water shall be drunk so as to vomit
the reagents.
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RT-7200 Auto Hematology Analyzer User’s Manual
zIf the hands or skins are touched with the reagents, they shall be immediately
washed to be clean using a large amount of clear water.
zThe waste products such as used test tubes, other instruments and consumables,
etc shall be appropriately handled as the medical waste products or infectious
waste products. If the pollution is caused by bloods, the infection might be
generated by pathogens.
zTake proper protection while replacing reagent
Voltage, connection and grounding of power supply
zThe power supply and grounding environment of this instrument are assured to
be good and stable.
zThe plug of the power supply shall not be inserted into the power socket which is
not available for the voltage requirement stated in the backplate of instrument, or
the misfire or electric shock might be caused.
zWhen the instrument is installed, the power supply cable and adapter as
accessories attached to the machine must be used and the good grounding shall
be assured, or the misfire or electric shock might be caused.
zDO NOT damage the insulation protection skins of the power cord. DO NOT pull
the power cord with efforts or hang heavy articles on it, or the short circuit or
open circuit might be caused.
zWhen the peripherals are connected, the power supply must be first cut off, or
the short circuit or open circuit might be caused.
zDo not open the side cover or panel while instrument is on, all it may cause
damage to some sensitive parts.
For safety purpose, users are not allowed to refit the instrument.
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RT-7200 Auto Hematology Analyzer User’s Manual
Contents
How to use this manual ......................................................................2
Main graphical representations used on this instrument ....................3
Warnings and security attentions .......................................................4
1 Instrument Introduction....................................................................9
1.1 Product introduction.................................................................................... 9
1.1.1 Product name: Auto Hematology Analyzer .......................................................... 9
1.1.2 Model: RT-7200 ................................................................................................... 9
1.1.3 Test items............................................................................................................... 9
1.2 Product technical parameters ..................................................................... 9
1.3 Instrument composition and structure......................................................... 9
1.3.1 Front view ............................................................................................................ 10
1.3.2 Rear view ............................................................................................................. 10
2 Installation .....................................................................................12
2.1 Instrument unpacking................................................................................ 12
2.2 Installation environment ............................................................................ 12
2.3 Power requirement ................................................................................... 12
2.4 Reagent .................................................................................................... 13
2.4.1 Connection of Lyse and cleanser ......................................................................... 13
2.4.2 Connection of diluent ........................................................................................... 13
2.4.3 Connection of waste ............................................................................................ 13
2.5 Connection of keyboard, mouse ............................................................... 13
2.6 Connection of external printer................................................................... 13
3 Sample test ...................................................................................14
3.1 Preparation before startup ........................................................................ 14
3.2 Startup ...................................................................................................... 14
3.3 Blood sample collection ............................................................................ 15
3.3.1 Venous blood collection ....................................................................................... 15
3.3.2 Peripheral blood collection ................................................................................... 15
3.3.3 Blood sample mixing............................................................................................ 15
3.4 Blood sample analysis .............................................................................. 15
3.4.1 Sample data edit .................................................................................................. 16
3.4.2 Blood sample counting......................................................................................... 17
3.4.3 Parameter prompt message................................................................................. 18
3.4.4 Histogram prompt message ................................................................................. 19
3.4.5 Adjust histogram .................................................................................................. 19
3.4.6 Print ..................................................................................................................... 20
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RT-7200 Auto Hematology Analyzer User’s Manual
4 Quality Control...............................................................................21
4.1 L-J QC ...................................................................................................... 21
4.1.1 L-J QC setting ...................................................................................................... 21
4.1.2 L-J QC run ........................................................................................................... 22
4.1.3 L-J QC list ............................................................................................................ 22
4.1.4 L-J QC chart......................................................................................................... 23
4.2 X-B QC ..................................................................................................... 24
4.2.1 X-B QC setting ..................................................................................................... 24
4.2.2 X-B QC list ........................................................................................................... 24
4.2.3 X-B QC chart........................................................................................................ 25
4.3 X-R QC ..................................................................................................... 26
4.3.1 X-R QC setting..................................................................................................... 26
4.3.2 X-R QC run .......................................................................................................... 26
4.3.3 X-R QC list ........................................................................................................... 27
4.3.4 X-R QC chart ....................................................................................................... 27
5 Calibration .....................................................................................29
5.1 Preparation before calibration................................................................... 29
5.2 Manual calibration..................................................................................... 29
5.3 Auto-calibration ......................................................................................... 30
6 History Data...................................................................................33
7 System Setting ..............................................................................36
7.1 General setting ......................................................................................... 36
7.2 Other......................................................................................................... 37
7.2.1 User setting.......................................................................................................... 37
7.2.2 Normal limits ........................................................................................................ 38
7.2.3 Panic limits........................................................................................................... 38
7.2.4 Units..................................................................................................................... 39
7.2.5 Communication .................................................................................................... 39
7.2.6 Department information........................................................................................ 40
7.2.7 Doctor information................................................................................................ 40
8 System Information........................................................................42
8.1 System status ........................................................................................... 42
8.2 System log ................................................................................................ 42
8.3 Statistic information................................................................................... 42
9 Shutdown.......................................................................................44
10 Service ........................................................................................45
10.1 Routine maintenance .............................................................................. 45
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RT-7200 Auto Hematology Analyzer User’s Manual
10.1.1 Startup and shutdown process ........................................................................... 45
10.1.2 Automatic rinse .................................................................................................. 45
10.1.3 Clean instrument surface ................................................................................... 45
10.2 Maintenance program............................................................................. 45
10.2.1 Back flush .......................................................................................................... 45
10.2.2 High voltage pulse.............................................................................................. 45
10.2.3 Drain chambers.................................................................................................. 45
10.2.4 Drain pipeline ..................................................................................................... 45
10.2.5 Remove Blockage.............................................................................................. 45
10.2.6 Prime.................................................................................................................. 46
10.2.7 Cleaning chambers ............................................................................................ 46
10.2.8 Concentrated cleanser soaking.......................................................................... 46
10.2.9 Check mechanics............................................................................................... 46
10.2.10 Debug .............................................................................................................. 47
10.2.11 Engineering ...................................................................................................... 47
10.2.12 Machine reset .................................................................................................. 47
10.2.13 Stop use........................................................................................................... 47
10.2.14 Replace reagent............................................................................................... 48
11 Troubleshooting ...........................................................................49
Appendix Name and concentration of toxic and hazardous
substance or element in product ......................................................50
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RT-7200 Auto Hematology Analyzer User’s Manual
1 Instrument Introduction
1.1 Product introduction
1.1.1 Product name: Auto Hematology Analyzer
1.1.2 Model: RT-7200
1.1.3 Test items
This analyzer is using impedance principle (colorimetry for hemoglobin measurement) to
categorize and count blood cells in blood. Test items are shown in Table 1-1.
Table 1-1
Full English name English abbreviation Unit(Default)
White blood cell count WBC 109/L
Lymphocyte count LYM# 109/L
Intermediate cell count MID# 109/L
Granulocyte Cell count GRA# 109/L
Lymphocyte percentage LYM% %
Intermediate cells percentage MID% %
Granulocyte Cells percentage GRA% %
Red blood cell count RBC 1012/L
Hemoglobin content HGB g/L
Hematocrit HCT %
Mean corpuscular volume MCV fL
Mean corpuscular hemoglobin MCH pg
Mean corpuscular hemoglobin
concentration
MCHC g/L
Red cell distribution width SD RDW-SD fL
Red cell distribution width CV RDW-CV %
Platelet count PLT 109/L
Mean platelet volume MPV fL
Platelet distribution width PDW %
Plateletcrit PCT %
Platelet–large cell ratio P-LCR %
White blood cell histogram WBC Histogram
Red blood cell histogram RBC Histogram
Platelet histogram PLT Histogram
1.2 Product technical parameters
Test principle: WBC/RBC/PLT: Impedance method; HGB: colorimetry
Aspiration volume: 9.8μL(Whole Blood), 9.8μL(Anticoagulant Peripheral
Blood), 20μL(Pre-diluted Peripheral Blood)
Test rate: About 2 min /ea.
Working environment: 15°C～35°C, relative humidity ≤80%
Store environment: 0°C～40°C, relative humidity ≤85%
Power supply: a.c.110V-220V, 50/60Hz
Input power: 96WATTS
1.3 Instrument composition and structure
The analyzer is composed of chamber, tubing system, computer control system, and
software.
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RT-7200 Auto Hematology Analyzer User’s Manual
1.3.1 Front view
Fig.1-1 Front view
①Display screen: Display the software interface
②Indicator light: green at startup, turn red when starting sample test, turn green after test.
③Feed key: built-in printer feeds paper outward
④Aspiration key: in sample analysis, press this key, the instrument will aspirate sample
⑤Printer cover: print paperinstalling position for built-in printer
⑥Sampling needle: use sampling needle to aspirate sample into chamber
⑦Aspiration key: in sample analysis, press this key, the instrument will aspirate sample,
function the same as key ④
1.3.2 Rear view
Fig.1-2 Rear view
1) Parallel port: printer interface
2) USB port 1
3) USB port 2
4) Network interface 1
5) Network interface 2
6) RS-232 serial port: to connect with data receiving devices
7) Keyboard interface: PS/2 keyboard interface
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RT-7200 Auto Hematology Analyzer User’s Manual
8) Mouse interface: PS/2 mouse interface
9) VGA interface
10) Power interface: to connect with external power supply
11) Waste port
12) Diluent port
13) Power switch: switch instrument power
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RT-7200 Auto Hematology Analyzer User’s Manual
2 Installation
2.1 Instrument unpacking
1. Unpack the instrument’s package and remove material for transportation. Please
keep original packing carton and packing material, in case you need to repack the
instrument in the future.
2. Take out the instrument from plastic package.
3. In accordance with packing list, make sure the packing carton content includes:
zRT-7200 Auto hematology analyzer
zUser’s manual
zPacking list
zPower adapter
zProduct COA
zPS/2 mouse
Note: Product packing feature is as per packing list, in case of
inconformity, please contact seller.
2.2 Installation environment
In order to ensure instrument work normally, please choose working place compliant with
following condition to place RT-7200 Auto Hematology Analyzer:
zNo direct sunlight;
zNo massive dust or powder;
zNo strong electromagnetic radiation;
zSufficiently large flat and solid desktop.
Note: Instrument working environment: temperature 15 ℃～ 35 ℃ , relative
humidity 80%.
2.3 Power requirement
za.c.110V-220V
z50/60Hz
z96WATTS
Warning: (1) AC power must be well earthed.
(2) AC power shall be stable, sharing with heavy-duty power
appliance is forbidden, rectified power supply is better
equipped.
(3) If there is smoke, smell or noise in instrument, immediately
shut off power, and contact distributor.
(4) When plugging power line, one must catch the plug itself,
instead of power line.
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RT-7200 Auto Hematology Analyzer User’s Manual
2.4 Reagent
The instrument needs to use Lyse, cleanser, diluents for measurement and maintenance. To
ensure accuracy of test result, please use reagents accompanied with instrument.
2.4.1 Connection of Lyse and cleanser
1) From reagent packing carton, take out lyse and cleanser respectively, open the
bottle lids, and place them in instrument reagent booth horizontally.
2) Insert plastic tube marked Lyse into the Lyse bottle and tighten the bottle cap.
3) Insert plastic tube marked cleanser into the cleanser bottle and tighten the bottle
cap.
2.4.2 Connection of diluent
1) Take out diluent catheter from the accessories bag.
2) Connect diluent catheter end to “Diluent” route adapter on rear panel of instrument.
3) Insert the other end of catheter into diluent bottle, and tighten the bottle cap.
2.4.3 Connection of waste
1) Take out waste catheter from accessories bag.
2) Connect waste catheter end to “Waste” route adapter on rear panel of instrument.
3) Clockwise rotate the bottle cap with waste catheter to tighten it on the waste bottle.
Note: (1) Reagent tubing must not be twisted, folded or rotated by
force.
(2) Please do not use expired reagent.
(3) The waste must be treated in accordance with related
national regulations, wear rubber gloves when disposing of
waste.
2.5 Connection of keyboard, mouse
1) Carefully take out keyboard and mouse from packing carton.
2) Carefully insert keyboard cable plug into socket marked “Keyboard” on instrument
rear panel.
3) Carefully insert mouse cable plug into socket marked “Mouse” on instrument rear
panel.
2.6 Connection of external printer
1) Insert one end of printer cable into printer USB or parallel interface socket.
2) Insert another end of printer cable into instrument USB or parallel interface socket.
3) Connect printer and AC power supply with power line accompanied to printer.
4) Switch on printer and the instrument.
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RT-7200 Auto Hematology Analyzer User’s Manual
3 Sample test
3.1 Preparation before startup
Before startup, the operator shall check by following steps to make sure system is ready:
1. Check if the diluent, Lyse and cleanser meet the need of current test. Check if the waste
bottle is full, tubing system is ok and without folded, all the connection is ok.
2. Whether instrument power adapter is safely and stably connected to power socket.
3. Check whether built-in or external printer paper is sufficiently installed in right position.
3.2 Startup
Switch on the RT-7200, indicator light is on, system begins to check component status,
transfer parameter needed by front end, run blank tests, as shown in figure below:
Fig.3-1 Startup program
After startup self-checking, go to sample test screen, as shown below:
Fig.3-2 Blank test result
The operator can run blank test at any time required. In sample test menu, press “Aspiration
Key” to start blank test. During test, the indicator light turns red, status column on top of
screen will display test status.
Blank test acceptable range is as follows:
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RT-7200 Auto Hematology Analyzer User’s Manual
Parameter Reference R ngea
WBC ≤0.2 × 109/ L
RBC ≤0.03 × 1012/ L
HGB ≤2 g / L
HCT ≤0.5 %
PLT ≤10 × 109/ L
If blank test result exceeds this range, operator has to repeat above test steps until test
result is acceptable. If after 5 runs, test result still cannot meet specification, please check
reagent and tubing connection, and use “Back Flush” function in “Service” module to
eliminate block hole.
3.3 Blood sample collection
Blood sample collection is classified as venous blood and peripheral blood.
Warning: Do not touch blood sample, QC sample and calibration sample
directly. Handling of these objects shall comply with related
operation instruction.
3.3.1 Venous blood collection
Venous blood can be collected by using vacuum negative pressure tube or ordinary method
under atmospheric pressure, anticoagulant must be dropped to all venous blood collection
containers in advance, usually EDTA.K2.2H2O is adopted as anticoagulant, with content of
1.5-2.2mg/ml.
3.3.2 Peripheral blood collection
zBlood sampling position:
For adults, the inner side of middle finger or ring finger tips of left hand is better; for
children above half a year old, middle finger is better; for infant below half a year old,
blood is usually sampled from thumb or outer side of foot bottom.
zBlood sampling method:
It shall be carried out in accordance with peripheral blood collection standard of health
authority. Peripheral blood collection normally adopts local centesis; the typical
collection method is to pierce through finger end. Blood tube uses 20μL constant
volume blood tube or bullet blood tube. It is recommended to collect no less than 30μL
blood to facilitate double check.
During blood sampling, if blood flow stagnates, one may slightly press farther end of
wound, never force around piercing hole. Avoid tissue fluid mixed with blood, hampering
test analysis result accuracy.
3.3.3 Blood sample mixing
Before test, blood sample must be thoroughly shaken and mixed, the recommended method
is: shake up and down, rotate test tube 3-5min, do not shake too violently.
Blood sample to be tested can only stored at room temperature, test shall be done in 4h,
prolonged storage, or inferior mixing will influence test result accuracy.
3.4 Blood sample analysis
Prior to sample analysis, it is recommended to take one QC test of instrument, see detail
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RT-7200 Auto Hematology Analyzer User’s Manual
operation in Chapter 4 “Quality Control”.
Warning: Instrument test object is blood sample; aspiration of other
substance may lead to instrument malfunction.
3.4.1 Sample data edit
In sample analysis, you can modify sample no. or input sample data. Modification after test
has to be done in “History Data” module, see detail operation in Chapter 6 “History Data”.
Click “Profile” button, Data Edit menu pops out as follows:
Fig.3-3 Sample data entry
¾Sample No.: to modify sample no., please input number within 8-digit length in sample
no. box. If this sample No. has existed in system, then the user will be asked to use new
sample no. Otherwise, the system will cover the existed test results. The system will
create sample number from 1 everyday.
¾Name: maximal 20 letters.
¾Sex: male or female may be selected, if no chosen, the system default is blank.
¾Age: for Year, Month and Day, just input one number. If multiple items are input, then if
year is available, then month and day will not be saved, if month is available, the days
will not be saved. Age range is 1-150, month range is 1-30, and day range is 1-90.
¾Sample type: anti-coagulating whole blood, anti-coagulating peripheral blood and
pre-diluted peripheral blood can be chosen.
¾Reference: general, male adult, male female, 14-18 years, 6-13 years, 3-5 years, 2
months-2 years, 8 days-1 months, the 1st week and user-define value can be selected.
The default is general.
¾Medical No.: patient medical record no.
¾Bed No.: number of sick bed of patient.
¾Department, sender, analyst and checker: directly input or select from dropdown box,
please see detail setting in Chapter 7 “system setting”.
Click “ ” to open soft keyboard, as shown below:
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RT-7200 Auto Hematology Analyzer User’s Manual
Fig.3-4 System soft keyboard
When soft keyboard is used, the cursor has to be moved into input box, then click to input.
“Caps” is to toggle between uppercase and lowercase, click “Close” to close soft keyboard.
Soft keyboards in other frames are used likely.
Click “OK” button, system will update or save sample data, click “Cancel” button, return to
sample test screen. Click “Last”,”Next” and “Save” button to realize the batch input.
3.4.2 Blood sample counting
You can select blood type by “Mode” button or select form the box at sample data edit menu.
There are three kind of blood type for selection, which are whole blood, anticoagulant
peripheral blood and pre-diluted peripheral blood.
If select pre-diluted peripheral blood mode, first add 20μl peripheral blood at the diluting cup,
then click “Diluent” button, a message box as following will appear:
Fig.3-5 Add diluent
Press the aspiration key will add 700μl diluent to the diluting cup to complete the dilution out
of instrument, the analyzer will aspirate 300μl pre-diluted sample for counting.
The test procedures are as following:
1. Put sample cup under sampling needle, press aspiration key, the instrument aspirate
blood sample, wait until sampling needle rises inside instrument, remove sample cup.
2. Instrument begins to analyze sample, frame top message box shows “Testing…”, after
test, parameter, test result and histogram will be shown as follows:
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RT-7200 Auto Hematology Analyzer User’s Manual
Fig.3-6 Blood sample counting screen
If “Print instantly” setting is selected, the instrument will print analysis result automatically
after test ends.
If ”Transmit instantly” setting is selected, the instrument will transfer analysis result to the
computer by RS-232 serial port after test ends.
If counting and analysis operation environment is below 15℃or over 35℃, the analysis
result of the blood sample will be unreliable; the “Temperature Low” or “Temperature high”
alarm will appear in message field of screen.
If block hole or bubble appears during counting and analysis, “Block hole” or “Bubble” alarm
will appear in message field of screen.
If the remain volume of reagent is not enough, the message field will appear “Reagent
volume not enough”, it will also indicate which reagent is not enough such as diluent,
cleanser and Lyse.
If test result exceeds system output range, parameter result will be shown as “---”, then
check validity of test sample.
Note: (1) If sample splashes out of sample cup, please make
necessary protection when cleaning, never touch sample
directly.
(2) Please check remaining reagent amount periodically, if
aspiration tube fails to aspirate thoroughly, please replace
reagent.
(3) Sampling needle movement may cause body injury, watch
out during aspiration.
3.4.3 Parameter prompt message
H+: indicates that parameter test result is higher than maximum of predetermined
pathogenic value.
L-: indicates that parameter test result is lower than minimum of predetermined pathogenic
value.
H: indicates that parameter test result is less or equal to maximum pathogenic value,
greater than maximum of normal value.
L: indicates that parameter test result is greater or equal to minimum of pathogenic value,
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RT-7200 Auto Hematology Analyzer User’s Manual
less than minimum of normal value.
3.4.4 Histogram prompt message
LF1: prompts that region on the left side of lymphocyte peak is abnormal, possibly because
of: platelet coagulation, giant platelet, plasmodium, nucleated red blood cell, non-lysed
red blood cell, abnormal lymphocyte, cryoglobulin.
LF2: prompts that lymphocyte peak and intermediate cell region are abnormal, possibly
because of: heteromorphic lymphocyte, plasma cell, atypical cell, initial cell, eosinophils
and basophils population.
LF3:prompts that the region between intermediate cell area and neutrophil peak is abnormal,
possibly because of: immature granulocyte, abnormal cell and eosinophils.
LF4: the region on the right side of neutrophil is abnormal, possibly because of:
granulocytosis.
PF1:the region on the right side of platelet is abnormal, indicating probable existence of:
large platelet, platelet aggregate, small red blood cell, cell fragment and fibrous protein.
PF2:the region on the left side of platelet is abnormal, indicating probable existence of: small
platelet cell fragment, red blood cell inclusion body and electronic noise interference.
3.4.5 Adjust histogram
When WBC, RBC, PLT automatic classification result fails to meet requirement, the operator
may adjust histogram. Procedure is as follows:
1. In sample test menu, click “Adjust” button, histogram selection frame pops up, as shown
below:
Fig.3-7 Histogram selection
Select histogram to adjust, click “OK” to return; click Cancel, then no histogram will be
chosen. To adjust histogram without external keyboard, click “ ” to open soft
keyboard.
2. Use mouse to choose type line, press “←” to move left, “→” to move right. Meanwhile
current volume is shown on left-move type line. WBC diagram has only 2 sign lines to
adjust, both sign lines of RBC and PLT can be adjusted.
3. After adjustment, click “OK” button, confirmation frame will pop up as below:
Fig.3-8 Adjust confirmation
Click “OK” button to save adjust result, click “Cancel” button to cancel this adjustment
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RT-7200 Auto Hematology Analyzer User’s Manual
result.
3.4.6 Print
Before sample test, please set up printer and print format in system setting module, see
detail in Chapter 7 “System Setting”. Click “Print” to print this test result.
Warning: During test, please do not replace reagent.
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