Reichert Univar User manual

Reichert Univar
Manual

Translated from the 11/1975 German language edition,
with slight modifications.
William R. Porter
San Marcos CA USA
2017
v 1.4

Notes
This is a very slightly-modified, new (2017) American English translation of the 1975
German-language version of the Reichert Univar microscope operating manual.
In a very few places, the text has been rewritten slightly for clarity. Any mistakes are
probably mine. No other Univar manuals or literature were used (nor indeed available) in the
production of this one. This manual was translated purely for the use of myself; it is being
made available to the public, free of charge, for their own use and enjoyment, with all the
usual disclaimers and the understanding that they do so at their own risk, etc., etc.
It was composed with the help of SoftMaker Presentations, Google Translate, and an
assortment of utilities, all on Ubuntu Linux.
The typeface is DejaVu Sans Condensed.

Table of contents
A) Setup and assembly.................………………..................................................................... A1
B) Univar transmitted-light setup
Transmitted-light brightfield microscopy……................................................................. B1
Transmitted-light darkfield microscopy…………............................................................. B3
Transmitted-light phase and anoptral contrast……....................................................…..B5
Transmitted-light microscope with polarized light …....................................................…B7
Transmitted-light interference contrast……………....……….………………….......................B9
Transmitted-light brightfield fluorescence…………………………………..................……….. B11
Transmitted-light darkfield fluorescence……………………………….……............…………… B13
Simultaneous contrast fluorescence with transmitted-light...……………………….....……. B15
Simultaneous darkfield fluorescence with transmitted-light ………......………............…. B17
C) Univar epifluorescence attachment
Immunofluorescence using epifluorescence ............……………….………………………...… C1
Conventional fluorescence and quinacrine using epifluorescence ......................…..….. C3
Detection of aromatic amines using epifluorescence ..........………………………..……...... C5
Mixed illumination: epifluorescence + transmitted-light darkfield, &
epifluorescence + transmitted-light darkfield fluorescence ...………….........…… C7
Mixed illumination: epifluorescence + transmitted-light phase and anoptral contrast .. C9
Mixed illumination: epifluorescence + transmitted-light interference contrast ..……..... C11
Mixed illumination: epifluorescence + transmitted-light polarization …………...……...... C13
D) Mirror & lamp houses and connections
Mirror house 4 ........…...…………………………………………………………………………..........D1
Mirror house 2 ........……...………………………………………………………………………..........D3
Lamp house 10 .....………………………………………………………………………………........... D5
Lamp house 25 .....………………………………………………………………………………........... D7
Lamp house 50 .....………………………………………………………………………………........... D9
Power supply housing .………………………………………………………….……………….......... D11
Power supply for 100W 12-volt halogen lamp .......…………………………….…………....…..D11
Power supply for HBO 200W/4 or CS 200W/4 mercury-vapor high-pressure lamp ......... D12
Power supply for CSI 250W/1 metal-halide short-arc lamp ....………………………….….... D12
Intermediate transformer...…………………………………………………………………………..... D13
Power supply for HBO 200W/2 mercury-vapor high-pressure lamp .….......……….……... D13
Power supply for XBO 450W xenon lamp .……………….....……………………...…………..... D14
Remote control device for the control unit to the XBO 450W xenon lamp .....…........…..D14

(E) Trimatic camera system ………………………………………………………………………............ E1
Installation and connection of the Trimatic camera system ………………………………….. E3
Operation ………………………………………………………………………………...………............ E5
Special cases:
Point measurement ..……………………………………………………………………..........E7
Fluor button ….....……..…………………...…………………………………………….......... E7
Double exposure .……………………………………………………………………...............E7
Half-frame photograph ...…………….…………………………………………………......... E7
Extension factors …………..........……………………………………………………………............ E8
Interruption of exposure ..……………………………..…………………………………………........E8
Lock Gate ………………………………………………………………………………………............... E8
Magnification in the film plane ..……………………………………………………………...…...... E9
Remote control ...………………………………………………………………………………............. E9
Micro Flash Terminal ...………………………………………………………………………...….........E9
Camera with lnternational camera back, format 4” x 5”..…….…………………………........ E10
Camera with Polaroid Pack film cassette, style 3 1/4” x 4 1/4" ..……………………………..E10
Automatic camera for the 24 x 36mm format ..……………………………………………….... E11
F) General notes
Observation tube ...…………………………………….………………………………………............ F1
Rotating stage No. 28 ............…………………………………………………………………..........F1
Coarse drive stop ....……………………………………………………………...……………............ F1
Condenser, coarse and fine adjustments ....…………………………………………………....... F1
Transmitted-light objectives ....…………………………………………………………………........ F2
Wide-field plan compensation eyepieces.....……………………………………………….…...... F2
Relay system .....……………………………………………………………………………….............. F3
Magnification changer................................…………...………………………………………...... F3
Slider slot........…………………………………………………………………………………...............F3
Measuring micrometers.................................................................................................. F3
Counting grids................................................................................................................ F3
Half-frame panels........................................................................................................... F3
Projection attachment ..…………………………………………………………………………..........F4
Automatic zoom & magnification indicator ……….……………………………………………... F4
Care of the microscope ………………………………………………………………………............. F5
Please note:
The mirror house 4 was used in the illustrations of the different working methods. If the mirror house 2 is
installed, the workflow remains the same, but references to non-existent knobs should be ignored. There
are left and right and upper and lower knobs, as well as the coaxial knobs for filters.

Erecting and assembling
Setting up:
Three handles are screwed into the base plate, and are used to lift the
microscope stand. The tripod weighs approx. 55 kg (121 lb) and should
be lifted by 2 people. The desk on which the Univar is positioned must
be stable, since the microscope with all of its associated gear can
weigh up to 120 kg (264 lb).The countertop must be hard and flat, so
that an 8mm air gap on the underside of the large lamps houses is
ensured for proper air circulation.
Remove the protective transport pieces. The necessary hex wrench to
unscrew the pieces is included.
a) Remove the red plate covering the projection prism by unscrewing
the two allen screws.
b) Remove the red protector in the relay housing.
c) Remove the red locking screw for the beamsplitter prism and
replace with the supplied 32mm long screw.
d) Remove the red protector on the focus rack, after unscrewing the
hex bolt and the slotted screw. Withdraw the protector after the rack is
slightly raised. Replace the slotted screw.
e) Do not move the coarse feed before the assembly of the stage
support.
A1

Assembly of the stage:
The stand has a dovetail mount and the stage support has a mating dovetail.
There are cutouts on each dovetail that allow the two to be fastened together.
First, unscrew the large screw with a coin slot, ➂, near the top of the stage
dovetail. Then, push the stage support onto the stand dovetail (you may have
to move the stage up and down to accomplish this using the recesses). Once
together, lower the stage support until the focusing lever (the end of which is
visible through the opening ➀) is centered behind the threaded hole for screw
➂. The table support is held in this position. Run the screw into the threaded
hole in the release lever.
Lower the stage support until the upper edge of the index ➁ is level with the
upper surface of the stand dovetail mount. Clamp the locking lever ➃. This is
the correct setting for the coarse-drive stop for regular transmitted-light
preparations on 1mm thick microscope slides.
Assembly of the condenser holder:
Turn the condenser turret to the illustrated position; the screw-in condensers
should be oriented in a front-back direction. Run the condenser mount with the
condenser coarse-feed to the lower stop position. Hang the condenser turret
transverse pin into the v-shaped notch on top of the stand's condenser mount
by tilting it back slightly, hanging by the pin, and letting it down gently. Then
clamp the condenser carrier to the mount by tightening the knurled control
knob ➄ in a counter-clockwise direction a partial turn.
Assembling the nosepiece carrier and the objectives:
Screw in objectives into the optics carrier (the 10x lens is screwed into the
threaded hole marked with a dot.) The other lenses are installed so that the
objectives are higher magnification when the rotation of the objective
nosepiece is in a clockwise direction. With the stage in its lowest position (with
coarse focus), insert the optics carrier into the guide on the microscope stand
up to the stop and clamp with clamping screw ➅.
Magnification changer (if available):
Remove the cap from the opening on the stand. Turn the knob of the
magnification changer position to 2.5 (arrow). In this position you can slide in
the the magnification changer on its dovetail until it stops.
Phase-ring knob (if available):
Remove the cap from the opening of the stand. Insert the phase-ring assembly
by pressing on the adjustment knob to slide it onto the dovetail, and clamp
with the captive cap-head screw.
A2

A3
Attaching the mirror housing:
Remove the back of the mirror housing, after loosening the four
corner fasteners by turning each fastener counterclockwise 1/4
turn.
Fasten the housing to the microscope stand with the captive
allen screw inside the housing (about a third of the way down in
the center) using a long allen wrench or other hex key tool. Do
this with care so no prism or filter is damaged.
Remove the protective covers and foam blocks for the sliding
mirror, rotating prism and oscillating mirror. Do not touch optical
parts with your fingers.
Insert the filter holder with IR-blocking filters in the filter holder
on the rear. The filter holder top is marked either X or H .
The filter holder X is used for working with the XBO 450W Xenon
lamp; for the HBO 200W mercury-vapor pressure lamp, use filter
holder H.
Replace the back panel of the mirror housing on the orientation
pin located above, and secure it with the four captive corner
fasteners by tightening them about 1/4-turn clockwise.
Be careful that the filters are not damaged.
Note! If the rear panel heat-blocking filters are not in place, do
not operate the xenon or mercury-vapor lamps, since the prisms
will be damaged by the heat from the lamp.
Mounting the lamp housings:
Remove the protective caps on the mirror housing and attach the
lamp housings with the screws at the back. See the description
of the lamp housing and the electrical connection on the lighting
equipment.
Zoom lighting:
The connection cable for the automatic zoom lighting is
connected between the microscope base and the power supply
for the 100W low-voltage halogen lamps.

A4
Projection attachment:
The projection tower is placed on the microscope
stand, with the cylindrical light inlet over the opening
of the stand.
Carrying handles:
The three carrying handles can be unscrewed from the
base of the microscope stand. The threaded holes are
provided with screw plugs.
Hand pads:
The magnetic hand rests are pushed on to the stand.
Eyepieces:
The eyepieces are inserted into the eyepiece tubes so
that the orientation pins engage in the grooves.
Photo Setup: The assembly of the photo setup is
described elsewhere.

B1
Transmitted-light brightfield microscopy
A) with halogen lamp:
When doing transmitted-light brightfield microscopy with the
halogen lamp, the controls of the Univar are set at the red-
dot positions.
⚪ Turn on halogen lamp with control transformer
⚫ Turn on relay (red dot)
⚫ Switch off Bertrand lens (pull out pin)
⚫ Turn off neutral density filters in the body (black dot not
visible)
⚫ Deactivate blocking-filter slide or rotatable analyzer (red
dot)
⚫ Switch off the compensator
⚫ Pull out interference-contrast main prism until it stops
(loosen the clamping screw on the left side first)
⚫ Place into position the 10x objective
⚫ Turn condenser turret to brightfield wide-field
⚫ Swing out the transmitted-light polarizer.
⚫ Set condenser fine-feed in the central position and move
condenser up to the stop with the coarse feed
⚫ Turn lower condenser turret to the empty opening (red
dot)
⚫ Switch off excitation filter for transmitted-light (red dot)
⚫ Switch off neutral density filters for transmitted-light (red
dot)
⚫ Switch off the auxiliary optics for wide-field condenser (red
dot)
⚫ Adjust collector for transmitted-light halogen lamp set (red
dot)
⚫ Adjust the photo system magnification changer to low
magnification (L, red dot)
⚫ Set the beam path to the camera (red dot, CAM)
⚫ Set the beam to 20% to the tube (red dot, CAM/PRO)
⚫ Switch the phase ring knob to empty (red dot)
⚫ Set the magnification changer to 1x (red dot)
⚫ On mirror housing 2, set the rotary prism on transmitted-
light with halogen lamp
⚫ Switch off color filters for contrast fluorescence (red dot)
⚫ On mirror housing 2 set excitation filter on red dot
⚫ Set sliding mirror for halogen lamp (red dot)
⚫ Turn on automatic zoom lighting (red dot)
⚫ Focus on a specimen with coarse and fine adjustment
⚫ Slightly close field iris diaphragm and focus on the image
with condenser fine adjustment in the specimen plane.
⚫ Center field iris diaphragm with centering screws on the
condenser turret. Then open field iris diaphragm slightly
larger than field of view
⚫ Close the condenser iris diaphragm so that the
microscopic image appears clear and high contrast. This is
usually the case when the oculars' back focal plane has
about 2/3 of their diameters brightly illuminated (check with
Bertrand lens)
⚪ When changing objectives, check the openings of the field
and the condenser iris diaphragms and correct if necessary.

B2
B) with high-performance lamp:
Adjustment of the microscope and the microscopic
image is done in the same way described for the
halogen lamp.
⚪ Turn on the desired high-performance lamp.
⚫ Turn the rotary prism for light from the HBO lamp
⚫ When working with mirror housing 4, switch exciter
filter to white dot. With mirror housing 2, red dot
⚫ Turn on collector for high-performance lamp
⚪ Use neutral density filters for transmitted-light
⚫ With the appropriate high-performance lamp
collector, evenly illuminate the field
Brightfield immersion condenser:
N.A.: 1.30
Free working distance: 0.42mm
Use: for all lenses from 4x to 100x.
When working with the 4x objective, the condenser
turret is set on 4. Suitable for all contrast methods.
Brightfield dry condenser:
N.A.: 0.90
Free working distance: 0.29mm
Use: for all lenses from 4x to 100x.
When working with the 4x objective, the condenser
turret is set on 4
Recommended for all investigation methods where the
immersion condenser NA=1.30 cannot be used and
immersion is not desired. Suitable for all contrast
methods except for contrast fluorescence.
Large field condenser:
Aperture: 0.12
Free working distance: 43.7mm
Use: for all objectives from 2.5x to 10x.
The condenser is permanently mounted in the turret.
When working with this condenser and with objectives
below 4x, the supplementary optics lever (if available)
in the microscope base plate is set to LP. To sharpen the
field diaphragm, the condenser is lowered first with
coarse feed to the lowest position, then lowered with
fine adjustment. The upper condenser turret is rotated
so that the mark LP is toward the observer, and for no
filtering, the lower condenser turret is rotated so the
white edge mark is under the LP

B3
Transmitted-light darkfield microscopy
A) with halogen lamp:
⚪ Switch to the halogen lamp with control transformer
⚪ Relay switch: red dot
⚪ Turn off the Bertrand lens (pull out pin)
⚪ Switch out the eyepiece neutral density filters (black dot not
visible)
⚫ With the immersion darkfield condenser, use the 40x objective
⚫ With the dry darkfield condenser, use the 25x objective
⚪ Switch off exciter filter for transmitted-light (red dot)
⚪ Switch off neutral density filters for transmitted-light (red dot)
⚪ Use darkfield condenser: apply immersion oil to the immersion
darkfield condenser
⚫ When working with the dry darkfield condenser and the Apo 25x
and Plan 40x objectives, the aperture insert is inserted into the
condenser.
⚪ Swing out the transmitted-light polarizer
⚫ Set condenser fine drive in the central position and move
condenser, with the coarse drive, up to the stop. With the
immersion darkfield condenser, use oil between condenser and the
specimen slide
⚪ Turn to an empty opening of the lower condenser turret (red dot)
⚪ Switch off the auxiliary optics (if available) for wide field
condenser (red dot)
⚪ Adjust the collector for transmitted-light halogen lamp (red dot)
⚪ Set the magnification lever in the photo system to low
magnification (red dot, L)
⚪ Redirect the beam path to the camera (red dot, CAM)
⚪ Set eyepiece light to 20% (red dot, CAM/ pro)
⚪ Set the phase ring knob to empty (red dot)
⚪ Set magnification changer to 1x (red dot)
⚪ Disengage blocking-filter slide or rotatable analyzer (red dot)
⚪ When using the mirror house 2, set the rotary prism on
transmitted-light with halogen lamp
⚪ Disengage the compensator
⚪ Interference contrast main prism: loosen the clamping screw on
the left side and pull out to the stop (red dot)
⚪ Switch off color filters for contrast fluorescence (red dot)
⚪ On mirror house 2, set excitation filter to red dot
⚪ Switch sliding mirror to halogen lamp (red dot)
⚫ Switch off the automatic zoom lighting (lever to the side)
⚫ Set manual zoom lighting to DF
⚫ Fully open condenser iris diaphragm. Focus on the specimen
⚫ Slightly close field iris aperture and focus on the diaphragm
image with the condenser fine adjustment. Frame the field iris
diaphragm in the middle of the field using the centering screws.
Then open the field iris diaphragm just past the field of view
⚪ When moving a different objective into position, the iris of the
objective can be closed to avoid glare by turning the the knurled
ring. This objective iris can be opened again for brightfield work.

B4
B) With high-performance lamp
The adjustment of the microscope and the microscopic image is
done in the same way as described for the halogen lamp
⚪ Switch to the high-performance lamp: switch on the desired
high-performance lamp with corresponding power supply
⚫ Redirect the light with rotating prism into the microscope for
transmitted-light
⚫ On mirror house 4, turn the exciter filter to the white dot. On
mirror house 2, set the exciter filter to red dot
⚫ Set sliding mirror for high-performance lamp. Engage the
neutral density filters for transmitted-light when needed
⚫ Evenly illuminate the field with the high-performance lamp
collector
Immersion darkfield condenser:
N.A.: 1.18 -1.42 oil
Free working distance: 0.38mm
slide thickness: 1.1 ± 0.1mm
Use: for all objectives from 40x to 100x
A cardioid condenser, fully suitable for fluorescence studies
Dry darkfield condenser:
N.A.: 0.7-0.9
Free working distance: 4.8mm
Slide thickness: 1.1 ± 0.1mm
Use: In combination with the condenser turret, can work with 25x
and 40x objectives
An aperture insert is used in the condenser when working with
the Apo 25x and Plan 40x objectives
The condenser is fully suitable for fluorescence studies

B5
Transmitted-light phase- and anoptral-contrast
A) with halogen lamp:
⚪ Turn on halogen lamp with control transformer. Turn on
relay (red dot).
⚪ Set Bertrand lens off (pull out the pin)
⚪ Turn off neutral density filters in the body (no black dot)
⚪ Disengage blocking-filter slide or rotating analyzer (red dot)
⚪ Disengage compensator
⚪ Pull out the interference contrast main prism to the stop,
after loosening the clamping screw
⚫ Turn to the 10x objective. All transmitted-light objectives
from 10x are suitable for phase- or anoptral-contrast
⚪ Switch out exciter filters for transmitted-light (red dot)
⚪ Turn off neutral density filters for transmitted-light (red dot)
⚫ Turn to the brightfield condenser. The following condensers
can be used (p. B2): Immersion condenser NA=1.30 and dry
condenser NA=0.90
⚪ Swing out the polarizer filter
⚫ On lower condenser turret, rotate to the phase ring 10Ph ,
corresponding to the 10x objective
⚫ Set the condenser fine feed in the central position and with
condenser coarse feed, raise the condenser up to the stop
⚪ Switch off supplementary optics for widefield condenser
(red dot)
⚪ Adjust the transmitted-light halogen lamp collector for even
illumination (red dot).
⚪ Set the photo magnification changer to L (red dot)
⚪ Redirect beam to the camera (red dot, CAM)
⚪ Set light for 20% to the ocular body (red dot, CAM/PRO)
⚪ Turn the phase ring knob (for the objective of 10x) either to
phase-contrast ring 10, or for the anoptral-contrast ring 10A
⚪ Set the relay magnification changer to 1x (red dot)
⚪ On mirror house 2, set the rotary prism for transmitted-light
with halogen lamp. Switch off color filters for contrast
fluorescence (red dot)
⚪ Set exciter filter on mirror house 2 to red dot
⚪ Set the sliding mirror switch to halogen lamp (red dot)
⚪ Turn on automatic zoom lighting (red dot)
⚪ Focus on a specimen with coarse and fine adjustment
⚫ Slightly close field iris aperture and focus on the image with
condenser fine focus.
⚫ Frame the field iris diaphragm in the middle of the field
using the centering screws. Then open field iris diaphragm just
past the field of view
⚫ Open the condenser iris wide open
⚪ When moving a different objective into position, it is
necessary to set the relay phase knob to the appropriate
phase or anoptral ring to match the new objective.

B6
B) with high-performance lamp
The adjustment of the microscope and the microscopic
image is done in the same way as described for the halogen
lamp
⚪ Switch to the high-performance lamp with corresponding
power supply
⚫ Set rotary prism for transmitted-light
⚫ When working with mirror house 4, set the white-coded
exciter filter. Working with mirror house 2, set exciter filter
to red dot
⚫ Set the sliding mirror for high-performance lamp
⚪ Engage the neutral density filters for transmitted-light as
needed.
⚫ Adjust the collector of the high-performance lamp so that
the field is just moderately bright
Phase ring knob with phase and anoptral annuli
The internal turret contains the phase and anoptral rings
(i.e., the diffraction or phase plates, which are built into the
objective lenses in ordinary microscopes) that the relay
system places into the optical path. For the 10x objective,
the phase ring with 10 and anoptral ring with 10A are used.
The rings of the other lenses are also marked with the
objective magnification. The position 0 of the knob, the red
dot position, provides an empty hole in the beam path.
Phase ring selection
The phase annuli (annular diaphragms) are in the lower
turret of the condenser. The correct centering of each phase
or anoptral annulus to the image of the corresponding ring
phase plate should be done after changing objectives. For
the ring aperture to be centered:
First as in A) described, set the illumination with 10x
objective. Set the lower condenser turret to 10Ph , and the
phase ring knob to 10. Carefully center the field iris
diaphragm.
The Bertrand lens pin is then pushed in so that the
superimposed images of the phase annulus and the
diffraction plate are visible.
By turning the knurled head of the Bertrand lens pin, the
lens is focused on the plate and the annulus. Using the two
centering wrenches ➀ on the underside of the condenser,
press the wrenches to engage, and then turn them
individually to shift the images and make them concentric.
The centering screws ➁ on the condenser carrier, which
serve to center the field iris diaphragm, must not be
adjusted during the above procedure.

B7
Transmitted-light microscopy with polarized light:
(A) with halogen lamp
⚪ Turn on halogen lamp with control transformer
⚪ Turn on relay (red dot)
⚪ Turn off Bertrand lens (pull out pin)
⚪ Turn off neutral density filter in the ocular body (black
dot not visible)
⚫ Insert analyzer and set to 0
⚪ Disengage the compensator
⚪ Pull out interference contrast main prism until it stops,
after loosening the clamping screw
⚫ Rotate to the 10x objective. Stress-free lenses must be
used for polarization-optical investigations
⚪ Switch off the exciter filter for transmitted-light (red
dot)
⚪ Switch off the neutral density filters for transmitted-
light (red dot)
⚫ Line up the brightfield condenser. The brightfield
condensers described on page B2 are available in strain-
free versions (P). Turn lower condenser turret to the empty
opening (red dot)
⚫ Turn condenser fine feed to the central position and
raise condenser with coarse feed up to the stop
⚪ Set the collector for transmitted-light halogen lamp (red
dot)
⚪ Switch off the auxiliary optics for wide-field condenser
(red dot)
⚪ Set the photo system magnification changer to low
magnification (red dot, L)
⚪ Direct beam path to the camera (red dot, CAM)
⚪ Set beam at 20% to the body (red dot, CAM/PRO)
⚪ Set the phase ring knob to empty position (red dot)
⚪ Set the relay magnification changer to 1x (red dot)
⚪ On mirror housing 2, set rotary prism for transmitted-
light with halogen lamp
⚪ Switch off color filters for contrast fluorescence (red
dot)
⚪ On mirror housing 2, set excitation filter to red dot
⚪ Set sliding mirror to halogen lamp (red dot)
⚪ Turn on automatic zoom lighting (red dot)
⚪ Focus on a specimen with coarse and fine adjustment
⚫ Close field iris diaphragm, then focus its image with
condenser fine adjustment
⚫ Center field iris diaphragm in the middle of the field
with centering screws. Then open field iris diaphragm just
larger than the field of view
⚫ Swivel the polarizer into place. The interference colors
are visible when observing a birefringent specimen
⚫ Close the condenser iris diaphragm to the usual
2/3-open position, highlighting the interference colors
⚪ For comparative observations with normal light you can
switch out the analyzer or the polarizer

B8
B) with high-performance lamp
The adjustment of the microscope and the microscopic
image is done in the same way as described for the
halogen lamp
⚪ Switch on the desired high-performance lamp with
corresponding power supply.
⚫ Redirect this light with rotating prism into the
microscope for transmitted-light
⚫ When working with mirror house 4, use white-dot
exciter filter. Working with mirror house 2, set exciter
filter to the red dot
⚫ Set sliding mirror for high-performance lamp
⚪ Use neutral density filters for transmitted-light when
needed.
⚫ Evenly illuminate the field with the respective high-
performance lamp collector
Rotating analyzer filter for transmitted-light
⚫ The analyzer filter can be used in the optics carrier
slide cavity (after removing the blocking-filter slide, if
that is in place)
⚫ Pushed in up to the stop (red dot showing), the
analyzer is out of the light path; pulled out to the stop, it
is in place
⚫ The analyzer filter can be rotated through 360°, by 10°
increments on the inner scale. The outer scale indicates
degrees by 1/10°
⚫ The polarization direction is north-south at the 0
position
A readjustment of the analyzer can be carried out as
follows: move the polarizer into place. Hold the
measuring drum of the analyzer at the 0 degree position,
and using a coin, turn the screw at the front of the
measuring drum until the field is quite dark
Transmitted-light filter polarizer
⚫ The swivel-out polarizer filter is located on the
underside of the condenser. The east-west polarization
direction is when swiveled into place
Bertrand lens
The Bertrand lens is used to view the rear focal plane of
the objective lens in the binocular tube or the projection
screen. It is used for polarization-optical investigations
(axis imaging observations) or phase-contrast
examinations (the phase plate to phase ring alignment).
Also can be used to check the front of some objectives for
damage or dirt.
To use the Bertrand lens, push in the pin until it stops;
turn the knurled end of the pin to focus on the objective
back focal plane. To replace the Bertrand lens to its
storage position, pull out the pin until it stops.
Compensators
Standard cross-section 6 x 20mm compensators can be
used in the compensator slide cavity.

B9
Transmitted-Light Interference Contrast
(A) with halogen lamp
⚪ Turn on halogen lamp power supply
⚪ Turn on relay (red dot)
⚪ Disengage Bertrand lens (pull out pin)
⚪ Turn off neutral density filters in the ocular body (no
black dot)
⚪ Remove blocking-filter slide or rotating analyzer (the
white marked blocking-filter can be left in the light path)
⚪ Remove the compensator
⚫ Slide the interference contrast main prism up to the stop
and clamp with the screw located on the left side
⚫ Rotate in the 10x objective; interference contrast studies
can be carried out with this magnification. Only IK low-
power lenses may be used
⚪ Remove exciter or color filter for transmitted-light (red
dot)
⚪ Engage neutral density filters for transmitted-light
⚫ Rotate to brightfield condenser on upper turret; the
following strain-free condensers (P) can be used: dry
condenser, NA=0.90; immersion condenser, NA=1.30
⚪ Swing out the polarizer filter
⚫ On lower condenser turret, rotate to the compensation
prism 10 IC
⚫ Set condenser fine feed in the central position and raise
condenser with coarse feed to the stop
⚪ Switch off the auxiliary optics for wide-field condenser
(red dot)
⚪ Adjust collector for halogen lamp (red dot)
⚪ Adjust the photo system magnification changer to low
magnification (red dot, L)
⚪ Redirect beam path to the camera (red dot, CAM)
⚪ Set ocular body light to 20% (red dot, CAM/PRO)
⚪ Switch off the phase ring knob (red dot)
⚪ Set the relay magnification changer to 1x (red dot)
⚪ For mirror house 2, set rotary prism for transmitted-light
with halogen lamp
⚪ Switch off color filters for contrast fluorescence (red dot)
⚪ On mirror house 2, set excitation filter to red dot
⚪ Set sliding mirror for halogen lamp (red dot)
⚪ Turn on automatic zoom lighting (red dot)
⚪ Focus on a specimen with coarse and fine adjustment
⚫ Close down field iris aperture and focus on the image
with condenser fine feed
⚫ Center field iris diaphragm with centering screws; then
open field iris diaphragm just larger than the field-of-view
⚫ Close condenser iris diaphragm so far that the
microscopic image appears clear and full of contrast
⚫ Adjust the interference contrast main prism by turning
the knurled screw, until the desired contrast, black and
white or colored, appears
⚫ When moving to stronger objectives, the similarly
marked IC prism is rotated in with the condenser hub

B10
B) with high-performance lamp
The adjustment of the microscope and the microscopic
image is done in the same way as described for the halogen
lamp.
⚪ Switch on the desired high-performance lamp with
corresponding power supply.
⚫ Set the rotating prism for transmitted-light.
⚫ When working with mirror house 4, use the white dot
exciter filter; with mirror house 2, red dot exciter filter
⚫ Move sliding mirror to high-performance lamp position.
⚫ Use neutral density filters as needed.
⚫ Adjust the collector for the high-performance lamp to
evenly illuminate the field.
Interference contrast prism for transmitted-light:
The interference contrast main prism is inserted into the
opening just above the front of the objective nosepiece, and
secured with the knurled screw at the side of the nosepiece.
To remove, loosen the knurled screw and withdraw the
prism.
An analyzer is built-in to the prism, so with the knurled stem
at the front of the main prism you can turn on black and
white or colored.
Interference contrast prisms:
The IC prisms are installed into the lower turret of the
condenser. They are marked with the objective
magnification and with IC. A dedicated prism is required for
each objective. A polarizer is built into each prism.
The lower condenser turret contains the prisms and phase
contrast rings; you can adjust only the ring aperture
position with the centering screws on the underside of the
condenser. A mistaken adjustment of the prisms is not
possible.